LOCUS       DAZKCC010000001       122852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000001 DAZKCC010000000
VERSION     DAZKCC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 122852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 122852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..122852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.001"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..976)
                     /locus_tag="ACG8VH_000001"
     CDS             complement(<1..976)
                     /locus_tag="ACG8VH_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9108603.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
     gene            complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG8VH_000002"
     CDS             complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG8VH_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9108604.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG8VH_000003"
     CDS             complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG8VH_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9108605.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2753..3391)
                     /locus_tag="ACG8VH_000004"
     CDS             complement(2753..3391)
                     /locus_tag="ACG8VH_000004"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP9108606.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG8VH_000005"
     CDS             complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG8VH_000005"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9108607.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG8VH_000006"
     CDS             complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG8VH_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP9108608.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG8VH_000007"
     CDS             complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG8VH_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP9108609.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7185..7925)
                     /locus_tag="ACG8VH_000008"
     CDS             complement(7185..7925)
                     /locus_tag="ACG8VH_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP9108610.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG8VH_000009"
     CDS             complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG8VH_000009"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9108611.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG8VH_000010"
     CDS             complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG8VH_000010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9108612.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG8VH_000011"
     CDS             complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG8VH_000011"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9108613.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG8VH_000012"
     CDS             complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG8VH_000012"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9108614.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG8VH_000013"
     CDS             complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG8VH_000013"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9108615.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG8VH_000014"
     CDS             complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG8VH_000014"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9108616.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14805..15089)
                     /locus_tag="ACG8VH_000015"
     CDS             complement(14805..15089)
                     /locus_tag="ACG8VH_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108617.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15107..15997)
                     /locus_tag="ACG8VH_000016"
     CDS             complement(15107..15997)
                     /locus_tag="ACG8VH_000016"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9108618.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16017..16235)
                     /locus_tag="ACG8VH_000017"
     CDS             complement(16017..16235)
                     /locus_tag="ACG8VH_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP9108619.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16205..17905)
                     /locus_tag="ACG8VH_000018"
     CDS             complement(16205..17905)
                     /locus_tag="ACG8VH_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9108620.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18172..18945
                     /locus_tag="ACG8VH_000019"
     CDS             18172..18945
                     /locus_tag="ACG8VH_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108621.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG8VH_000020"
     CDS             complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG8VH_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP9108622.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19661..19763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19769..20740)
                     /locus_tag="ACG8VH_000021"
     CDS             complement(19769..20740)
                     /locus_tag="ACG8VH_000021"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9108623.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG8VH_000022"
     CDS             complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG8VH_000022"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9108624.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG8VH_000023"
     CDS             complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG8VH_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP9108625.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23695..24519)
                     /locus_tag="ACG8VH_000024"
     CDS             complement(23695..24519)
                     /locus_tag="ACG8VH_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP9108626.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24492..25172)
                     /locus_tag="ACG8VH_000025"
     CDS             complement(24492..25172)
                     /locus_tag="ACG8VH_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9108627.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25195..26133)
                     /locus_tag="ACG8VH_000026"
     CDS             complement(25195..26133)
                     /locus_tag="ACG8VH_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9108628.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG8VH_000027"
     CDS             complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG8VH_000027"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9108629.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG8VH_000028"
     CDS             complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG8VH_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9108630.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30097..30903
                     /gene="proC"
                     /locus_tag="ACG8VH_000029"
     CDS             30097..30903
                     /gene="proC"
                     /locus_tag="ACG8VH_000029"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9108631.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG8VH_000030"
     CDS             complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG8VH_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP9108632.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG8VH_000031"
     CDS             complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG8VH_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9108633.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34107..35348)
                     /locus_tag="ACG8VH_000032"
     CDS             complement(34107..35348)
                     /locus_tag="ACG8VH_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP9108634.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG8VH_000033"
     CDS             complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG8VH_000033"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9108635.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36375..37298)
                     /locus_tag="ACG8VH_000034"
     CDS             complement(36375..37298)
                     /locus_tag="ACG8VH_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP9108636.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38037..39323)
                     /locus_tag="ACG8VH_000035"
     CDS             complement(38037..39323)
                     /locus_tag="ACG8VH_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9108637.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39316..40584)
                     /locus_tag="ACG8VH_000036"
     CDS             complement(39316..40584)
                     /locus_tag="ACG8VH_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9108638.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40836..41297
                     /gene="mscL"
                     /locus_tag="ACG8VH_000037"
     CDS             40836..41297
                     /gene="mscL"
                     /locus_tag="ACG8VH_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9108639.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41356..41802)
                     /locus_tag="ACG8VH_000038"
     CDS             complement(41356..41802)
                     /locus_tag="ACG8VH_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9108640.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41895..42140)
                     /locus_tag="ACG8VH_000039"
     CDS             complement(41895..42140)
                     /locus_tag="ACG8VH_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP9108641.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42255..42833)
                     /locus_tag="ACG8VH_000040"
     CDS             complement(42255..42833)
                     /locus_tag="ACG8VH_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9108642.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42910..43440)
                     /locus_tag="ACG8VH_000041"
     CDS             complement(42910..43440)
                     /locus_tag="ACG8VH_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP9108643.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG8VH_000042"
     CDS             complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG8VH_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9108644.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44297..44692)
                     /locus_tag="ACG8VH_000043"
     CDS             complement(44297..44692)
                     /locus_tag="ACG8VH_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP9108645.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG8VH_000044"
     CDS             complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG8VH_000044"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9108646.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG8VH_000045"
     CDS             complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG8VH_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP9108647.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(46720..46959)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG8VH_000046"
     CDS             complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG8VH_000046"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9108648.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48586..49743)
                     /locus_tag="ACG8VH_000047"
     CDS             complement(48586..49743)
                     /locus_tag="ACG8VH_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP9108649.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG8VH_000048"
     CDS             complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG8VH_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9108650.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51210..51737)
                     /locus_tag="ACG8VH_000049"
     CDS             complement(51210..51737)
                     /locus_tag="ACG8VH_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9108651.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51967..52773
                     /gene="proB"
                     /locus_tag="ACG8VH_000050"
     CDS             51967..52773
                     /gene="proB"
                     /locus_tag="ACG8VH_000050"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9108652.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52770..54017
                     /locus_tag="ACG8VH_000051"
     CDS             52770..54017
                     /locus_tag="ACG8VH_000051"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9108653.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54093..54437
                     /locus_tag="ACG8VH_000052"
     CDS             54093..54437
                     /locus_tag="ACG8VH_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108654.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54554..54880)
                     /locus_tag="ACG8VH_000053"
     CDS             complement(54554..54880)
                     /locus_tag="ACG8VH_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108655.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55495..56697)
                     /locus_tag="ACG8VH_000054"
     CDS             complement(55495..56697)
                     /locus_tag="ACG8VH_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9108656.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56814..57329
                     /locus_tag="ACG8VH_000055"
     CDS             56814..57329
                     /locus_tag="ACG8VH_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9108657.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57528..57974)
                     /locus_tag="ACG8VH_000056"
     CDS             complement(57528..57974)
                     /locus_tag="ACG8VH_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9108658.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACG8VH_000057"
     CDS             complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACG8VH_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9108659.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59853..60482)
                     /locus_tag="ACG8VH_000058"
     CDS             complement(59853..60482)
                     /locus_tag="ACG8VH_000058"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9108660.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60466..60930)
                     /locus_tag="ACG8VH_000059"
     CDS             complement(60466..60930)
                     /locus_tag="ACG8VH_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9108661.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61417..62685)
                     /locus_tag="ACG8VH_000060"
     CDS             complement(61417..62685)
                     /locus_tag="ACG8VH_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9108662.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(62916..63653)
                     /locus_tag="ACG8VH_000061"
     CDS             complement(62916..63653)
                     /locus_tag="ACG8VH_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9108663.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(63720..64289)
                     /locus_tag="ACG8VH_000062"
     CDS             complement(63720..64289)
                     /locus_tag="ACG8VH_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9108664.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            64417..65289
                     /locus_tag="ACG8VH_000063"
     CDS             64417..65289
                     /locus_tag="ACG8VH_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9108665.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            65631..66503
                     /locus_tag="ACG8VH_000064"
     CDS             65631..66503
                     /locus_tag="ACG8VH_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9108666.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            66500..68458
                     /gene="nagE"
                     /locus_tag="ACG8VH_000065"
     CDS             66500..68458
                     /gene="nagE"
                     /locus_tag="ACG8VH_000065"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP9108667.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(68790..69641)
                     /locus_tag="ACG8VH_000066"
     CDS             complement(68790..69641)
                     /locus_tag="ACG8VH_000066"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9108668.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            69764..70231
                     /locus_tag="ACG8VH_000067"
     CDS             69764..70231
                     /locus_tag="ACG8VH_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP9108669.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(70494..70976)
                     /locus_tag="ACG8VH_000068"
     CDS             complement(70494..70976)
                     /locus_tag="ACG8VH_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9108670.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71076..71939)
                     /locus_tag="ACG8VH_000069"
     CDS             complement(71076..71939)
                     /locus_tag="ACG8VH_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9108671.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            72537..73412
                     /locus_tag="ACG8VH_000070"
     CDS             72537..73412
                     /locus_tag="ACG8VH_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9108672.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(73557..73709)
                     /locus_tag="ACG8VH_000071"
                     /pseudo
     CDS             complement(73557..73709)
                     /locus_tag="ACG8VH_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(73977..75308)
                     /locus_tag="ACG8VH_000072"
     CDS             complement(73977..75308)
                     /locus_tag="ACG8VH_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9108673.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(75457..76170)
                     /locus_tag="ACG8VH_000073"
     CDS             complement(75457..76170)
                     /locus_tag="ACG8VH_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9108674.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            76345..77769
                     /locus_tag="ACG8VH_000074"
     CDS             76345..77769
                     /locus_tag="ACG8VH_000074"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9108675.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            78068..78775
                     /locus_tag="ACG8VH_000075"
     CDS             78068..78775
                     /locus_tag="ACG8VH_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9108676.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            78762..79085
                     /locus_tag="ACG8VH_000076"
     CDS             78762..79085
                     /locus_tag="ACG8VH_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9108677.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(79174..80211)
                     /locus_tag="ACG8VH_000077"
     CDS             complement(79174..80211)
                     /locus_tag="ACG8VH_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9108678.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(80532..81515)
                     /locus_tag="ACG8VH_000078"
     CDS             complement(80532..81515)
                     /locus_tag="ACG8VH_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP9108679.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(81930..82700)
                     /locus_tag="ACG8VH_000079"
     CDS             complement(81930..82700)
                     /locus_tag="ACG8VH_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9108680.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(82693..83628)
                     /locus_tag="ACG8VH_000080"
     CDS             complement(82693..83628)
                     /locus_tag="ACG8VH_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9108681.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(83944..85593)
                     /locus_tag="ACG8VH_000081"
     CDS             complement(83944..85593)
                     /locus_tag="ACG8VH_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9108682.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(85710..88313)
                     /locus_tag="ACG8VH_000082"
     CDS             complement(85710..88313)
                     /locus_tag="ACG8VH_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9108683.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(88325..89374)
                     /locus_tag="ACG8VH_000083"
     CDS             complement(88325..89374)
                     /locus_tag="ACG8VH_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9108684.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(89400..89714)
                     /locus_tag="ACG8VH_000084"
     CDS             complement(89400..89714)
                     /locus_tag="ACG8VH_000084"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9108685.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(89730..90176)
                     /locus_tag="ACG8VH_000085"
     CDS             complement(89730..90176)
                     /locus_tag="ACG8VH_000085"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9108686.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(90177..92099)
                     /locus_tag="ACG8VH_000086"
     CDS             complement(90177..92099)
                     /locus_tag="ACG8VH_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9108687.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(92311..93207)
                     /locus_tag="ACG8VH_000087"
     CDS             complement(92311..93207)
                     /locus_tag="ACG8VH_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9108688.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(93200..93880)
                     /locus_tag="ACG8VH_000088"
     CDS             complement(93200..93880)
                     /locus_tag="ACG8VH_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9108689.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(93881..94663)
                     /locus_tag="ACG8VH_000089"
     CDS             complement(93881..94663)
                     /locus_tag="ACG8VH_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9108690.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(94656..95573)
                     /locus_tag="ACG8VH_000090"
     CDS             complement(94656..95573)
                     /locus_tag="ACG8VH_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9108691.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(95737..95991)
                     /locus_tag="ACG8VH_000091"
     CDS             complement(95737..95991)
                     /locus_tag="ACG8VH_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108692.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            96848..100075
                     /gene="fss3"
                     /locus_tag="ACG8VH_000092"
     CDS             96848..100075
                     /gene="fss3"
                     /locus_tag="ACG8VH_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP9108693.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            100188..100502
                     /locus_tag="ACG8VH_000093"
     CDS             100188..100502
                     /locus_tag="ACG8VH_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9108694.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            100515..100889
                     /locus_tag="ACG8VH_000094"
     CDS             100515..100889
                     /locus_tag="ACG8VH_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9108695.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            100899..101234
                     /locus_tag="ACG8VH_000095"
     CDS             100899..101234
                     /locus_tag="ACG8VH_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFP9108696.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            101317..102657
                     /locus_tag="ACG8VH_000096"
     CDS             101317..102657
                     /locus_tag="ACG8VH_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9108697.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            102735..103409
                     /locus_tag="ACG8VH_000097"
     CDS             102735..103409
                     /locus_tag="ACG8VH_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9108698.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            103402..103566
                     /locus_tag="ACG8VH_000098"
     CDS             103402..103566
                     /locus_tag="ACG8VH_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108699.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            103642..104784
                     /gene="dcm"
                     /locus_tag="ACG8VH_000099"
                     /pseudo
     CDS             103642..104784
                     /gene="dcm"
                     /locus_tag="ACG8VH_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            104774..105064
                     /locus_tag="ACG8VH_000100"
     CDS             104774..105064
                     /locus_tag="ACG8VH_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108700.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            105323..106507
                     /gene="mobT"
                     /locus_tag="ACG8VH_000101"
     CDS             105323..106507
                     /gene="mobT"
                     /locus_tag="ACG8VH_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9108701.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            106504..106641
                     /locus_tag="ACG8VH_000102"
     CDS             106504..106641
                     /locus_tag="ACG8VH_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9108702.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            107387..109297
                     /gene="ltrA"
                     /locus_tag="ACG8VH_000103"
     CDS             107387..109297
                     /gene="ltrA"
                     /locus_tag="ACG8VH_000103"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9108703.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            109401..109625
                     /locus_tag="ACG8VH_000104"
     CDS             109401..109625
                     /locus_tag="ACG8VH_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108704.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            109638..110141
                     /locus_tag="ACG8VH_000105"
     CDS             109638..110141
                     /locus_tag="ACG8VH_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9108705.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            110201..110590
                     /locus_tag="ACG8VH_000106"
     CDS             110201..110590
                     /locus_tag="ACG8VH_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9108706.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            110577..113024
                     /locus_tag="ACG8VH_000107"
     CDS             110577..113024
                     /locus_tag="ACG8VH_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9108707.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            113029..115152
                     /locus_tag="ACG8VH_000108"
     CDS             113029..115152
                     /locus_tag="ACG8VH_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9108708.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            115149..116153
                     /locus_tag="ACG8VH_000109"
     CDS             115149..116153
                     /locus_tag="ACG8VH_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9108709.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            116172..117083
                     /locus_tag="ACG8VH_000110"
     CDS             116172..117083
                     /locus_tag="ACG8VH_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9108710.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            117270..117526
                     /locus_tag="ACG8VH_000111"
                     /pseudo
     CDS             117270..117526
                     /locus_tag="ACG8VH_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(117743..117958)
                     /locus_tag="ACG8VH_000112"
     CDS             complement(117743..117958)
                     /locus_tag="ACG8VH_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108711.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(118207..118425)
                     /locus_tag="ACG8VH_000113"
                     /pseudo
     CDS             complement(118207..118425)
                     /locus_tag="ACG8VH_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(118720..120159)
                     /locus_tag="ACG8VH_000114"
     CDS             complement(118720..120159)
                     /locus_tag="ACG8VH_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9108712.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            complement(120747..121379)
                     /locus_tag="ACG8VH_000115"
     CDS             complement(120747..121379)
                     /locus_tag="ACG8VH_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFP9108713.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            complement(121728..>122852)
                     /locus_tag="ACG8VH_000116"
     CDS             complement(121728..>122852)
                     /locus_tag="ACG8VH_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9108714.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT        36256 a        24997 c        22411 g        39188 t
ORIGIN      
        1 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
       61 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
      121 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
      181 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
      241 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
      301 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca gttgcttgtt
      361 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
      421 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
      481 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
      541 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
      601 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
      661 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
      721 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
      781 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
      841 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
      901 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
      961 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
     1021 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
     1081 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
     1141 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
     1201 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
     1261 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
     1321 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
     1381 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
     1441 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
     1501 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
     1561 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
     1621 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
     1681 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
     1741 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
     1801 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
     1861 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
     1921 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
     1981 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
     2041 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
     2101 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
     2161 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
     2221 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
     2281 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
     2341 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaatc
     2401 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
     2461 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
     2521 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
     2581 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
     2641 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
     2701 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
     2761 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
     2821 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
     2881 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
     2941 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
     3001 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
     3061 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
     3121 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
     3181 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
     3241 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
     3301 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
     3361 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
     3421 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
     3481 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
     3541 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
     3601 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
     3661 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
     3721 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
     3781 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
     3841 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
     3901 ttggccctaa tgtgatattt gggacaaatc ggccatccat tacatctacg tggatgtaat
     3961 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
     4021 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
     4081 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
     4141 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
     4201 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
     4261 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
     4321 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
     4381 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
     4441 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
     4501 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
     4561 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
     4621 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
     4681 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
     4741 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
     4801 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
     4861 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
     4921 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
     4981 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
     5041 tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg
     5101 atatatttta ttcagcactt attttgaact ttctgaggtg ctagctgatg aacttgtgga
     5161 gctggaagat tcttcttttg aagagttgct tgtgctactg gagctacttg agctgctaga
     5221 actgtctgaa gagtcttttt cagaactaga agacgctgga tctggtcctt tagagtaaat
     5281 tacgttcaca accattcctt tggtaattgt cgttcctgcc cctggtactg tacgaatcac
     5341 tttatcttta tccaccgtgt ttgaatattc ggaagtctca tgcccgatat attctgcacc
     5401 gacactagct aaataacttt gtgcttcgct ttttgtatag ccggagatat cattcaattt
     5461 aggctctgtt ccttggctta cgacaaaatt gatgtaagtt tcttctgcta caacttcacc
     5521 cgaggctgga tcctgactga tgatcgaacc tgatggtaca gtgtcgctat agtcataagt
     5581 agcactaata ttattgatat tgaatccttg tgtaacaagc caattacgaa cagtttcgta
     5641 gttttctcct acatgactac tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt
     5701 aatcgtgtct gttttaggat cgacttctgt atcttctgat ggagattgcg aaatgatcat
     5761 tccgctttct acctctgaac tgaattcctc ttctttttga atcaagtcag cagaaaaccc
     5821 taattctttc aaacttgctt cggcatcttc ataactttta ccactgtaat catctaattt
     5881 gatttttttc gtgccgctac taatatacag agtgacttcg cgtttttgct tgactgtcgt
     5941 tcccgctgcc gggtctgtct tcactacatt accttcttcg attttgtcat ctgcaatttc
     6001 ttctgttttt gttgctactt ctaatttcgc ttttgcgagt gtttctctag cagaagcttc
     6061 agaaagatca gaaacatcag gaacaacaac ttctcctcgc ccgccagaag caaagaatac
     6121 agctgttccg attcctaaaa cagcaagaat agctaatagg atcagccacc attttttctg
     6181 tcctttcttt ttaggatcag gcagaacttc ttcctcctct ggtaaggttt cgttttctgg
     6241 cttgtctttt ggcagaggca tagatttgaa tgaatcaggc attggtgcat cttccgtaag
     6301 gggtgtgata acttttgttt cattatccat cacatgaggc tgccattttt cttcgtttgc
     6361 acgagctggt gataatactg tagaaagatc atcagacatt tcctcggctg acttataacg
     6421 gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt
     6481 tggatcaagt gcttttatag atggaagatc atcttgaaaa tgttttaaag caattgttac
     6541 agcagattca ccatcaaacg gtacgctacc cgtcaacatc tcataaagga tgatccctaa
     6601 agcataaata tctgactgct tcgttgccat acttcctctg gcctgctcag gggacagata
     6661 atgaacagat cctagcatcg tattcgtctg cgtgatcgat gtttcagata aagcgatagc
     6721 aatcccgaaa tccgtgattt ttacaacacc ttcattatcg atcagtacgt tttggggttt
     6781 caaatctcgg tggatgattt gatggttatg agctaaagaa atagcagaaa ggatctgctg
     6841 cataatattt actgctgttt cataaggaac aggataatgt gtttggatat atcgtttcaa
     6901 gtccattcct ttgacgtatt ccatcaccag atactgcatg ttgtcttctt caccaacatc
     6961 gtaaacacta acaatgtttg gatgaacgag ttctgttgct gctaaagctt ctctctgaaa
     7021 tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt aagactttaa ctgctacatc
     7081 acgatccaag attaaatcgt gcgccaaaaa tacatttgcc ataccaccgc taccgatatt
     7141 tccagtgatt tgataacggc catttaattt tttgcccaac tcgatcatac actgtcctcc
     7201 ttatattcga taagaagaac ggtgatatta tccgctccgc ctgcttcatt ggcacgatca
     7261 atcagctcag ttactttatg agacaatggg ccttcttgat tgatgatcgt tcctatttct
     7321 tcttcagaca gcatgttcgt caagccatct gaacataaca gcagtcgatc ttttagttgc
     7381 caaagatgag tagaaacatc tacttcgact gttccaggca tgccgacaga acgtgttaaa
     7441 atatttttcc ttggatggtt cactgccatt tcttcgctga tttctcctga tttgactagt
     7501 tcattgacta aagaatgatc ctccgtcaat tgcatgattt tatcttcacg aatcaaataa
     7561 gctctgctat ccccaacatt ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg
     7621 attgttgttc ccataccgtt atattccggc tgttcttgtc cacgctgata aatcagcata
     7681 ttttcttctt gaatcgattg aatgaaccat tgggcgattt tttcatcgtc aacagcttct
     7741 tgtttttccc aagcttcgcc tagattcgtt accgccatct gactggcaat gtctccggca
     7801 cgatgaccac ccatcccgtc tgctaataca gcaagcttga gacctgaacg gctggtaaat
     7861 acactggcat agtcttgatt tgtgttacgt cgtcttccta catctgattg atattcaatc
     7921 tgcatttttt cacctcatta ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa
     7981 aactgatgcg gatacaaagt caacattttc tcttcgattg atgattgtag cataggattg
     8041 gtcaaaacat caatcctctc aaaatcaggg tgtctttcca agaaagcaga aacaacgtct
     8101 tggttttctt cttttaagat cgtacaagtg ctataagtca gaataccgga agactttaaa
     8161 gttggtgcgc agctttctaa aatttccaat tggatttttg gtaaattcgc taattcatcc
     8221 ggctgttttt tataacgaat atctggtttt cgccgaagca gcccaagacc tgaacaaggc
     8281 gcgtctacta aaatccgatc aaactgctct gcttcgaact gtttactgac ttctctagca
     8341 tccatctttt gagcgtagac agcagaagag acacctagac gtgctgcatt atcttcaatg
     8401 agtttgactt tgtgttcgtg tacatctaaa gcgaccactt ctccaccgca tgacggatct
     8461 aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg cacaggcatc taagacatga
     8521 tgttcaggct cgatttgcat agacggcgct acaagcatcg aactttcatc ttggatcgtc
     8581 atgacgccat cgtggaacaa tgagctagaa gctaaatgcc cttttttcgc tactataccg
     8641 tatggactga cttggctttt ttcagctgtg atctcttctt cttgcaaaac agtgatggcc
     8701 tgatctctgg aaagtcggcg tgtatctact cgggcactag catggctagg ctgatatagc
     8761 gataaaccga gcttttcagt ttcttcgtat ccgatttgat ccaccagtcg tttcgtcatc
     8821 catagaggca ggctgatttt gacagaaagt ctttcgatag gatcagcaat gccttgcaga
     8881 tctggtgctc cttgacgctg ataattcctc aagacaccat ttacaaattt ccctgtccca
     8941 ggatttcctt ttacttttgc gatttcgacc gcctcgttta aaatagcatg tgcagggact
     9001 ttatctaaaa attctaattg gtatacagaa agataaagaa gcatcttgac ccagtcatcc
     9061 actttttttg ctttagcgat caatggtgca acgtaaaatt ccaatagtcg ctgatggcta
     9121 atcgtaccat agatcaattc tgtaaataaa cccacatctt tttgatttag ctcgctttgt
     9181 ttgatcatct cattcaacaa aagattcgag taggcgccac ccttgttcac tcgctctaat
     9241 gtcagtaatg ccatatatcg tactgatttt ttcatttttt tagggatttt tttcattatt
     9301 tcacctgctg accaatctga acttgctgac cgcttccgtt aaggaaatca tgtacggcct
     9361 gttttccttt accagctggc tgtaattcat tgatggctaa gacggtctgt ttaccgcaag
     9421 caatccatag attttttttg tctttccgga tgatcgtacc aggtgctttg tctgtagttt
     9481 ctgccaatac ttcaaccgat aataatttcc agcgtatgcc ctcataggtc gtgaatgcaa
     9541 ttggccaagg acgcattccg cgtacttggt tgtcaatttc ttctgcagtt ttatgccaat
     9601 cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt tgcttctgac tcgtcttgag
     9661 gtactggttt ttgtccatcc aaaatattgg gcaatgtttc tagcaatagc tttcttccta
     9721 aaaggctaag cttttcaaac atcgtcccta catcatcttg tttagtgatt ggtatgcttt
     9781 cttgcgcata gattccgcct gcatccattt ttttgatcat ttccatgatc gttacaccag
     9841 tttctttctc cccattcatg atggcataat gaacaggcgc acctccccga tatttaggaa
     9901 gtaaagaagc atggacattg attgccccta attttggtac ttgcaataac ttttcaggca
     9961 agaattggcc aaatgccgca gtgacgatca catcaggcac caattcaatg atttcttcca
    10021 tttctggcga accggagatt ttctctggct gcagcacacg gataccgtgc ttcagagccg
    10081 ctttttttac cggtgtcggc gtaattactc gtttccgccc aactggacgg tcgggctgtg
    10141 ttaccactgc ctggatctcg taacctgatt ctatcagccc ttcaagaatc ggcacagaaa
    10201 aagcaggtgt gcccataaat actagcttag tcatcaagat gttcctccat atattcttca
    10261 agttcttctt ccgggatctg ctgtatcatt ttctcaatga acaagatacc gtctaagtga
    10321 tcgatttcat gctggaaggc acgggctaaa tagccaaaag ccgtcacttc tatctcttct
    10381 ccgtctcgat cataataacg cacggtcact tcatctgctc gcttgaccgt tccatagaca
    10441 tgcggaatac tgagacaacc ttctacgtca aggctttcac cttttgcttc gatgatttct
    10501 gggttaatca gttcaaattt ctcgccttca tctacttcga tcacagcaat tctcttgttt
    10561 tgtcctactt gaggagcagc gatccctatt ccgtcattcg caatcattgt ctcatataaa
    10621 ttgtctaata aagcgattgt ctcgtccgtg attacatcga ttggctgagc agttcttttt
    10681 aatttatcat tagggtggat caatatagga tagcgcataa tttggccctt tcttaaataa
    10741 agtttaatgg ttcgttgtca attgccacaa aaagaccttg tcgctgttct ttctggcttc
    10801 cattcaaaat ttcatccaaa acatgaggaa gattcggttc atgtttatat ttgatgatta
    10861 gttgataata atatcggttt ttcactctca aaatcatgct gggcgttggc cctaatagca
    10921 agctttgagg gcttaacact tcttttagct gattggcaat ctgaaagatt ttctttgccg
    10981 ctacttgttc ttcaggatgg ctggcggtta ttttcacagt aaagtaataa ggaggatagc
    11041 ctccacgatg ccgtacatgc atttcctgct gataaaactg ctcataattt tgttttttag
    11101 ccaattctat ggcgtaatgt tgaggattga atgtttggat cacgacttcg cctgcttttt
    11161 ccgctcgccc tgctcgtcct gaaacttggg taagcaattg gaatgtatgc tcactagaac
    11221 gaaaatccgg taaattcagg gaggtatcag cattcaacac cccaactaat gtcacttctg
    11281 gaaagtccaa acctttagca atcatttgtg tccctaataa tatatctgct tcttttgcac
    11341 cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg agtggtatct acatccatac
    11401 gcaaaatcct agcttgcgga taaagttctc ggagttcctc ttctactttt tgagtacctg
    11461 ttccatagta gcggatttta ttgccgccac aattaggaca gcgattggga ataggttctt
    11521 catgtccgca ataatggcag cgcattgaac gtgtatccat atgcagtgta agagagatat
    11581 cgcaattagg acatggaaga acaaatccac agtccctaca catcacaaaa gacgaatatc
    11641 ctcgacgatt gagcagtagg acagtttgtt cttttttgtc taatcgattt tgtatttttt
    11701 cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg aaactcttca cgcatatcta
    11761 cgatctcaat agctggaagc tgtgcagctg ctttggctcg gtggtttaac tgcaataact
    11821 gataaacgcc tttttgagct cgtgctcttg attctaaaga gggcgtcgcg cttcctagaa
    11881 ctattgggca atgatgatat ttactgcgcc aaattgctag atctctcgca tgataacgcg
    11941 gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc gataatgatc agcccgatat
    12001 ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac tacttgcgct tcttcccgct
    12061 caatcttgcg ccattcatca tatttttcac cttgggaaag tccgctatgt aagaccgcaa
    12121 ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg cggagtcaat gcgatttcag
    12181 gcactaacat aatagccgtt ttattttctg acaaaacatc agcgattgcc tgaagatata
    12241 cttctgtttt cccgctgcca gtgattccct ccagcaaaaa gacctgatct ttttgctgct
    12301 gacctgctgt gatgatttgt tcgacagcat ttttttgttc tgcattcagt tccaatgccg
    12361 tcgtttggtc aaatactcgg tctttataag ggtcgcgata acgttctgat tctataaact
    12421 ccagccagcc cttttctttt ccttgattca agacagcagt actgattcct ttatttttga
    12481 aataagcaat tggttcttct tggctcagtt cagataaaca caaaagaagt tctttttgtt
    12541 tcttggcatt ttgccgtaat tttgcccatt cttcttcgat ttgcttttca gttaacaaag
    12601 aacggaccaa acgaatcgtt tttactttat ttcgtgtatg gacttcatat ttgatatcta
    12661 ctaattgctc ttggcgccag cgaatcaatt gagaaaggct gccttcttct tctgcttcct
    12721 tccaggaaat cacttctcgc tgatcaaaca atacctgaac ttctggtgtg tctgccaaag
    12781 gatagatcaa tttatcatat tctgctttca tcacactcgg aagcatcgtt tgtagacaag
    12841 tgattttgaa tgcgtaagta atttttttca tataatcagc taatgaaagt aattcttcat
    12901 tgaccaccgg agcaaggtct agcaaacgaa taactgcctt caaagatgga ttagctgatt
    12961 cttctgattt ttggatagcc ataacaaatc cttggacatg acgattgcca ttaccaaaag
    13021 ggacttctac tcgcatcccg acttgcaaag ctgtttccat ttcagaggga accaaataag
    13081 tgaatggctg atcggtctgc atagtaggta cgtccacaat gacttcagct gttaatgtca
    13141 tggttcgttt cctccttttt ttgtcttttc ttatcaagcg acaaaaagtt ccttgcctat
    13201 tttactagag atcgatggat ttgtcttatc ttctgccaaa tcctaacaaa aaattaacag
    13261 cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca cagccttttc taccagacaa
    13321 taagtggcga atgtttgaaa aggaaacagc tgggtttgct tttcttttta gatgttcgct
    13381 ctatttttct ttattacatt ttttgctctt cacggatacg tgattccaat tcgtgttgtt
    13441 cacgctcttt ttgtgcttga cgttcctctt cttccatctt caaacgttca cgttttaatt
    13501 caggatgtgg atcattgact acatttcctg catcgatttc ttctaaagct tgaccgacgc
    13561 tttttactga atcaaaagaa tctagagtag gctgtgcact tgcatccaat tcatgcgcgc
    13621 gtttacttgc taaaatcact agagaatatt ttgaatttac acgatctaat agtgagtcaa
    13681 ttgaaggttt taacatcata agactacatc tcctttaaca ttttaatata tttaccgatt
    13741 acgcgatcta ctcgaaaatg ttcactggca ataatgtttt tgattcgttc aactgctagc
    13801 ggcacttcat catttacaac agcatagtcg taaagagcca tcatttcgat ttcttctttt
    13861 gcaactttca ttcgtttttc gattacttca tctgcatctg ttcctcggcc tacgatccgt
    13921 gattttaatt ccgctaaatc tggcggagtc aaaaagatga atacaccatc gggcactttt
    13981 tctttgactt gttttgctcc ttggacttca atctccaaga aaacatcttt tccttcatcc
    14041 aatgtcttgt tgacatatga caaaggagtt ccataataat tcccgacata ttctgcgtat
    14101 tccagcatct ctccagcttc gatcattgat tcaaactctt ctttgcttcg gaagaaataa
    14161 tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag tcattgagat cgaatactga
    14221 aaatcattat cctcactctc aaaaattgct tttcgtactg tgccttttcc tacaccggat
    14281 ggaccagaca acacgattaa taatccacgc tctgacatga cgacgtcctt tcaactagtc
    14341 tttttgtact tatcgtttat tttgcacttt ttttctgcaa ctttcaaggt tatctttaat
    14401 tatagctaga aattggtaaa aaagctaccc tgtaccaaga ggtcgtaaaa aagctgtcct
    14461 gcatcaagta ataagaacag ccaaacataa acagcttctc atgtttttcg tttggttggg
    14521 gttattatcg cagatgcaag cttttgaacg ccgtgtataa gaggtcgtga agaagccgat
    14581 tagtttcgag tatgaagaaa aattttgtga aaatagtttc tcacattttt gcataaattt
    14641 ggcttaatac cgagaaaatg gtttttgaac atcacttatt caaaataaaa aaagattgtg
    14701 acacgaattt tgtcacaacc tttaaatgta ttcctagagc aaagaagcta agcgaagaaa
    14761 tccaattctg tttatttatt gtatttcttt gttcagcatc cttgctaaaa gcccatattg
    14821 gacaaagtat tcactacacc catgatctca tcttttcgag aacgtgcttt ttctgtcgga
    14881 tcttctacct cttctgtgtc aattcgaaca ataaatttcc gattcatcgt cacgtaagca
    14941 ccgtcgttga tcaaaataaa cagttcgttc tcattatagt ctgcacaggc atctttccat
    15001 acgtcaaagc cgtttttttc agaagggata tcccgtttaa ccacagtttt tccacttaat
    15061 tcgtggaacg ttacgtcata atatttcatt atctctacct ctttcgtcat tcaatatttt
    15121 gtacttgttc tctgattttt tctaagatcg tcttagactg gacgacttga tttttaattt
    15181 cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg attcatttct tggatcagga
    15241 aatctaattc ccgacctaca ggacattgtg cttcgataag ttcatctagt ttttgtacat
    15301 ggatcgccag acgatccagt tcctcgtgaa tatctccgcg ttccaacaag atggctagct
    15361 ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa ttcctccagc tttttttgat
    15421 accttatttg aaattccttt tcatagattt ctacaaaact atcgagtttt tttagtactt
    15481 ctttgaattc ttcagtattc tttttcaaca ctaaagctaa agcttgacct tctttttctc
    15541 gactcgcctc aatagaatca agtgcgcttt tcaatgtatc tagagctaac tgtgccaatt
    15601 cttccatgct ctcttccggt ttttcttcta ccgttacgaa gttttcgtcc agcacgaagc
    15661 gttccgccat ccgactaatt gacacttgat tttgactacc gtatcgttgt tgaattccgt
    15721 ctgtcaattg agtgataagt gaatcaatca attcccagtt gatgacgatc tgtttttcgt
    15781 ttttgccaat ataagctaga ttcacaaata cttcgattcg accgcggctt aactgctgtt
    15841 taataagctg acgaaaatcg ttttcaacaa agttcaggat tttaggactt cgtatttgaa
    15901 catctagaaa acgctggttg acacttttta tttcaatatc taactgatac gcttttgttt
    15961 cgcggatggc tttgccaaaa ccagtcatac ttttcattcg acagggtcct tcctcattac
    16021 atattttcta ttgctttttg cagttgttca aactcttcgt tcgtcaatgt caggcctttc
    16081 cccatttttt catggtctgg cgcccaatct cgaagatcga attttggcgg gcgaccattc
    16141 cagctgacta ggttcagttc tttacgccac cccttgttgt tttctgacaa tatagcgatt
    16201 tcttctacga tttcatatga aaattcttgt gccatggttg ttcctccttg agataatata
    16261 aatataggat cagtaatgtg tcaactggtt gcttaagcaa ttcatgctgc aaacgatcct
    16321 tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt cgtgcgctca ttttgtattt
    16381 tttgctggta ttttcgatac cttcgccatt cttcagtact taattgacca atttgtgcca
    16441 ataaatcatc cggcatactt tttatatctt gttggcagat catttgaatc atcatgtcta
    16501 cttgatagat ttcattgggc agtaaaaaat gtttttttgc taaaagttct agcatcgcca
    16561 atccgcatgt ctctggtacg accctgttct tatataaatt acgcagtttt tgccaaaatt
    16621 ttatgctttt gaaaaaagat aattctaact gaatttcttt tgttaagtaa gacaaaaatc
    16681 ctcgccagta tgttcttgca ggtttaacag aatgaaatgt ataccagttc ccatagtaat
    16741 agcctaatcg ttttttcatt tccagatagc ctaattgtaa atttaattgg ttcctttggc
    16801 tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg acatttccaa tggataaaaa
    16861 tgtctggcat acgaattttt ttcgctgggc ctggcccttg cacatctacg acaaatgcct
    16921 ctgttgctcc tttgttaatc gcaatatcaa taggtatttt gttgcgatac ccaccgtctg
    16981 catatttatt ttttccgatc tttcgataag ccatagcggg ataaaatgaa gcagaggcga
    17041 gtatccaatc tggtatttct tctgtttttg tctgttggat atcagtcact acttccgcta
    17101 gttctggcag tttagtagac accgtatata aacgtatttt gggattatgt ttgagtaagg
    17161 acaaatccaa attgtctttg accatctttt ccaatggagc agaagaaacc ccgccttcaa
    17221 ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa gcgttcacga aggtcttcaa
    17281 ctgcagccat ttctggtaac gcaagcactt gtcttgtcga cagctttttc cataaattca
    17341 cagctttttt catatctcct tgtaaaatca atgcgccgtt caacgcacct accgaggtac
    17401 ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc ctgccacaca ccaatctgat
    17461 aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt atgatagacc agctttttat
    17521 ttaattgtgt ggttccttga tagccgtttt tatgaaaaat acgcaaccat tcttctgata
    17581 gaggagattg aaataaaaaa caacagtttt ctttaataaa aaaacgagcg caagcttcca
    17641 tataccgaaa aaattttttc cattcaaaag tttcaccttc aatcaacaga ttattaaaaa
    17701 taacatctcc tctactttta gcaatagtaa aatagacggt atattttttc gatactgctt
    17761 taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc tctactttct tgatgacttt
    17821 gaaaaaatgg aaggggttgt tttgcttgct ggataggcga atacagcgac attttctgtg
    17881 aaaagtaaac tttttttatc tccatcgcgc tctccttttt cttcaagtat accagtacgt
    17941 cataaaaagg tcatgagtct cgtattcgac tttgtagatt ccataagaaa acaattcata
    18001 aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat taattgaaaa tgagagtgtt
    18061 ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa aagaaacaag cttttttaat
    18121 gcaaatgttg taaaataaaa gagattaaga ggaaataaga ggaggaacat gatgactgag
    18181 tcagtttatg tacatattga taccacaagt aatgctgttt taacaaaagg gctgactgct
    18241 actgactttg caaacagtat cgtgcatttt cctcaaaact tgctcttgct tgatccatct
    18301 gcttctgctg gagaatatga atcccataca gggctgaaag taatccgtgg tacagaaaat
    18361 attcagcgtt tcttttcctc ttctcgtaac aggcgtggat tcgatgaatt gaagtggatc
    18421 gactttactg atttaacgat gctgaaagaa ttgacacctt tagaaatttc agaattatta
    18481 tattttggtc atatgaaaac acatcttcat tcgccgtttt tttataaact acagaataat
    18541 tttgtatact tcgatcttaa tgatcagctg aatcggattt attatcgcta tttagatgaa
    18601 ttttatagga tttttgctag taaattaagt tcgatcttgt cagataagat caatgtaaaa
    18661 aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca gtgtggaatt attgaaagaa
    18721 atgcgtaatt tgatgcagga agggatcatt ttttcttttg atcaagttgg tttgatcgat
    18781 ggtaaatatc gtatcccaat ctatgtgatt gaagattata tttggaaatt aaaaggcacc
    18841 cggtatcgag aagaggaaat catcgctact ttaatctact ctgtcaccga tcgacactgg
    18901 caattggtag aagatgccga tctttcttat ctgccattga agtagatcta tttcttatca
    18961 aaaaataaaa aggaaacaag aaactaggac atgattctgt ctcaagtttc ttgcttcttt
    19021 tttatttaca gctcctgtcg tttatctggc acaaaaatcg aaggagtcgt ttgttggatc
    19081 cctaataaaa cgatgatcgt tacaatagct gtactcaacc cgctggcacc attcattacg
    19141 aaagaaaaga gccaaggatt catcccccat agtgcatacg ctccccagaa aaccactcct
    19201 gcaataaaat gccaaaaata tctggaaagt gcgccaatca atgcaccaaa cacgatgctt
    19261 tttctaattt gtttcgtatt atcgtctaca tagccttttt ggacatttga agccatgagc
    19321 cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa tcaacacttg aacgacactt
    19381 aaataataaa cttgtcccaa cggaaaatgt aggatgcccc aaatgaaacc tgccagcatc
    19441 cctgcctttg tccctcttct taacgcaaaa agagtcaatg gaatctgccc taatgaaata
    19501 gaaaaactgc tgccgatctg gatcggaatc aacgacaaca ccatcgctaa cgctgcaaca
    19561 atcgttcctt ctacccaaac tctatttttt cccataaaaa aacttcctcc tttgaataaa
    19621 aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa cagctgttta ttcacaattc
    19681 ctacgctcgc ataaccgata caggtacgaa gggtttagaa atagattcaa tctcagccca
    19741 attggactcc cctttgtgag tttatttatt atttattttt tgtttcaaac aatggactaa
    19801 ctggtttgtt ttcatggata cgtttgattg catctcccat tagtccgcca acacttactt
    19861 catcgatttt gtcgatttta cgatcttctg gaagatagat cgagtctgta acaactagac
    19921 gctcgatagc tgaatcttct attctttgta aagcagggcc ggataatact ggatgtgtac
    19981 atgaagcata cacactagtt gcaccagctt ctttcaaagc attggcagcc aatgtgatcg
    20041 tacctgctgt atcgatcata tcatcgatca gtacacaagt tttgcctttg acttgtccaa
    20101 tgatattcat tacttctgca acatttgctt ttggacggcg tttgtctatg atcgcaatcg
    20161 gagatttcaa aaattccgct aatttacgtg cccgtgtgac accaccatgg tctggtgaaa
    20221 caacgacaac atcatcgcct ttgattcctt tttctaagaa ataattggca ataagtggcg
    20281 cacccatcaa atgatcgact ggaatatcaa agaaaccttg gatttggact gcatgaaggt
    20341 ccaaagtcag cattcttgtt gcaccagctt tttgaatcat gtttgctact aatttcgctg
    20401 tgattggttc ccgtgcacgt gctttacggt cttgacgcgc atagccataa taaggcatga
    20461 ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc aatcatgatc agcaactcca
    20521 tcaagttatc attgactggg ctgctcgttg attgaataac atagacatgc gatccacgaa
    20581 tactttcttc aatgtttact tgaatttctc catcactaaa ttgagtaaca gatgattttc
    20641 ctaactcgac gccgactgct gctgcaattt tctctgctaa tgggcgattg gaatttaacg
    20701 caaaaatttt taatcttggg tcaaagtaat gttttgacat ggggacctcc actttctttt
    20761 tcacaactct agaattctac ctaataaata gtagtcatat tttttaaata aatcaagtga
    20821 ttttgcaaaa gtcttagttt agataaggga gtttttttgc atatccttct ttattgactt
    20881 gtcttgctct cgcaattgcc agcgcatatt ctggaatatt gtctgtaatt gttgacccgg
    20941 cagcaatcga actgttttta gctacctcga caggtccgat gatattcgtg ctagaaccaa
    21001 taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt tccatcatag ttgacgaaga
    21061 caacaccaca tccgacattg atttcttctc ccagcgtagc atcaccaaca taagtcaagt
    21121 gaccgacttt agttcctttg ccgatttgag catttttcac ttccacaaag tttccgatat
    21181 ggacgttttc gccaatttca gcttttggtc gtaaatgtgc ataaggtccc acatctgcat
    21241 gtttcttgac atggctgctt tcaatgactg agttttcaat gacgacgtga tcctcaatcc
    21301 tactgtctac gatttttgaa tgtgacccga tgacacaatc agaaccaatg actgtatttc
    21361 cttggatttg tacacctgct tcgatcaatg tgtctggacc gatttctacg cctgcatcga
    21421 tgtatgtcgt tgctgaatcg acaaagctta cgccattgac catatgcatc tggttaattc
    21481 gctttctcat gatctcattt gctttagcta acgcgatccg atcattgact cccattgatt
    21541 cttcaaaatc atccgtttga taagcagcaa ctgtatggcc ttcttctttt aagatttcga
    21601 tgatatctgt tagatagtat tcaccttgag cattgtttgt tccaactttg cttaacgcat
    21661 cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt gatttcttgt acaagagctt
    21721 cttctggtgt tgcatctttt tgttccacga ttttttctac gatcccaata tggtcacgga
    21781 tgattcgtcc atatcctgtt gggttttctg cttgtgccgt caagatcgtt gcacttgcat
    21841 tttttccttg gtgatattca aaaagattgt tcagcgtttc agtagtcaat aatggtgtat
    21901 cgccactgat aactaacgtt gttccttttt ttccttccaa gaaagaagca gcttgcaaaa
    21961 cagcatgtcc tgttcccaat tgttctgctt gtaacgcata tttactgcgt tctcctagtt
    22021 gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat cacttcatct ggtttcgttt
    22081 cactgacccg attaatgata tgttctacca taggtttacc agaaacggga tgtaaaactt
    22141 tatataattt tgacttcatg cgggtacctt tccctgccgc caaaataatt gcataacgtg
    22201 cgtccaactg cgtcactcct aaatatagtt tgctgatttc agtttatccc agtataccca
    22261 ttttttcaac aaaacaagtg attttctttt atcccagcgt tttctggata aaaaaataga
    22321 tagaccataa cgggcctatc tatttggtaa ccggtgctct cctgccaact cctcggtcac
    22381 aagtcacact actcataaaa catgaaaagc tgttttttga aatcgtttct tgtgacttag
    22441 agttaaacgg taggcttaca acctcttcat taaacgtttt tcaatcagct gaccttcggt
    22501 attagctcaa taaacggcaa aatatgagga gcaatttttc cgtttattga gctaatactc
    22561 aggacaggac gctgatttca caacctctag ataaacggtg ttcaaaagcc agtttctcgg
    22621 tattaagcca aatttatgaa aaaatgtgga aagccatttt cacaaaattt ttcttactac
    22681 tcgaaactaa gcggcttttt cacaacctct tattctgaaa aataatttcc tggcacaacc
    22741 gtaatatttt tcgtttgagt atctacatct ttgacataaa gcagtgacgt ataatcatcg
    22801 atggcgcgat gaccgttgaa ttttgattcg gcaaatacag tgatgccaac aagttctgat
    22861 tcgaattctt caatcaagct cttcattccg ttgactgttc cgccgccttt catgaaatca
    22921 tccactacaa ggacacgtga accacgtttc aaacttctct tagacagttc catcttttca
    22981 attctttcag aagatccaga cacatagttg acacttactg ttgatccttc tgttattttt
    23041 gagtcccggc gtacaataac gaatggcgca ttcagataat aggacacagc ttgggcaata
    23101 ggtacgcctt ttgtggctac agtcattaca gcatcgattt tttcaccaag gtatttagaa
    23161 gcaatgattc ttccaacttg acgcagcagt tcaggttctc ctaacaaatc agagagataa
    23221 acatatcctc ctggaagcag acggtcttgt tctgatagac gttctgctag agattcaata
    23281 tattttttcg cctcttcata tgaaatttca gggatgaaca atacaccacc tgcagcaccc
    23341 gggattgttt ccaaggttcc ataccctcgt tctttaaatg ttttcttgat gattcccaaa
    23401 tcttcactga tagaagattt agcagattca taacgttcgg caaagcttgt taaggaaatc
    23461 aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa ctagacgttc actgcgacgt
    23521 actttcacct attccaactc cttttaagaa attttatcgt tttacattat ataagttttt
    23581 cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata gttcggtatt tattaaaata
    23641 aaaagaagcc tataaaaaat ttttcgtttt tataaggctt cttttcttta taatttagtt
    23701 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
    23761 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
    23821 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
    23881 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
    23941 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
    24001 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
    24061 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
    24121 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
    24181 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
    24241 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
    24301 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
    24361 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
    24421 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
    24481 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
    24541 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
    24601 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
    24661 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
    24721 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
    24781 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
    24841 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
    24901 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
    24961 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
    25021 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
    25081 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
    25141 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
    25201 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
    25261 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
    25321 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
    25381 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
    25441 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
    25501 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
    25561 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
    25621 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
    25681 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
    25741 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
    25801 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
    25861 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
    25921 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
    25981 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
    26041 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
    26101 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
    26161 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
    26221 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
    26281 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
    26341 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
    26401 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
    26461 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
    26521 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
    26581 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
    26641 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
    26701 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
    26761 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
    26821 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
    26881 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
    26941 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
    27001 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
    27061 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
    27121 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
    27181 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
    27241 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
    27301 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
    27361 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
    27421 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
    27481 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
    27541 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
    27601 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
    27661 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
    27721 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
    27781 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
    27841 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
    27901 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
    27961 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
    28021 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
    28081 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
    28141 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
    28201 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
    28261 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
    28321 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
    28381 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
    28441 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
    28501 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
    28561 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
    28621 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
    28681 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
    28741 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
    28801 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
    28861 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
    28921 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
    28981 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
    29041 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
    29101 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
    29161 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
    29221 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
    29281 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
    29341 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
    29401 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
    29461 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
    29521 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
    29581 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
    29641 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
    29701 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
    29761 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
    29821 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
    29881 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
    29941 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
    30001 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
    30061 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
    30121 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
    30181 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
    30241 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
    30301 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
    30361 ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat
    30421 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
    30481 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
    30541 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
    30601 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
    30661 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
    30721 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
    30781 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
    30841 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
    30901 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
    30961 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
    31021 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
    31081 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
    31141 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
    31201 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
    31261 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
    31321 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
    31381 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
    31441 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
    31501 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
    31561 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
    31621 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
    31681 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
    31741 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
    31801 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
    31861 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
    31921 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
    31981 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
    32041 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
    32101 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
    32161 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
    32221 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
    32281 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
    32341 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
    32401 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
    32461 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
    32521 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
    32581 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
    32641 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
    32701 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
    32761 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
    32821 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
    32881 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
    32941 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
    33001 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
    33061 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
    33121 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
    33181 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
    33241 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
    33301 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
    33361 gcgcgtcctc caggagtgat ttctggattc gcaaacatca cgcctacttt ttcacggatt
    33421 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
    33481 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
    33541 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
    33601 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
    33661 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    33721 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    33781 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    33841 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    33901 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    33961 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    34021 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    34081 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    34141 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    34201 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    34261 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    34321 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    34381 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    34441 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    34501 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    34561 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    34621 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    34681 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    34741 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    34801 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    34861 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    34921 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    34981 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    35041 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    35101 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    35161 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    35221 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    35281 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    35341 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    35401 aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca
    35461 gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt
    35521 tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa
    35581 acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg
    35641 aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca
    35701 ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct
    35761 tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca
    35821 agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc
    35881 cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg
    35941 caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt
    36001 ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg
    36061 tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg
    36121 ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt
    36181 tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa
    36241 tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga
    36301 ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa
    36361 actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga
    36421 ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt
    36481 gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt
    36541 tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat
    36601 acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact
    36661 gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat
    36721 tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag
    36781 gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca
    36841 gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat
    36901 cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc
    36961 attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc
    37021 aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca
    37081 gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca
    37141 ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa
    37201 gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact
    37261 cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt
    37321 tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt
    37381 tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt
    37441 tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg
    37501 tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt
    37561 ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac
    37621 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc
    37681 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt
    37741 acacggtgtt ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga
    37801 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt
    37861 tgattacttc gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt
    37921 tattttctca ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga
    37981 ggttcctact acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat
    38041 tttggtctca tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc
    38101 agtacgtctt tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg
    38161 ccaaataaac tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa
    38221 tattgtccaa gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca
    38281 tcagcgaatc caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta
    38341 tctccgctga agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg
    38401 tcatcgataa ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc
    38461 atttggaaca acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc
    38521 aatgtgtcta atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat
    38581 gcactttgtt tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt
    38641 ggagggaaat tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc
    38701 atcttaccaa ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta
    38761 tacaagtctt gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc
    38821 aatgggtgtg taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg
    38881 tcttcataca tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct
    38941 gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc
    39001 gtcgtagaaa acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct
    39061 tgtttcccaa ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa
    39121 aaatgggcaa ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg
    39181 ttatcgatcg aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta
    39241 ggcaacaaat acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt
    39301 tgctcatatt ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa
    39361 ttcgatttgc tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa
    39421 ccattcttca tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat
    39481 tgccgcttgc ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg
    39541 agacagttcc aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag
    39601 atgaagtact tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg
    39661 gaacgtatcc acgctactag aggcataata agctagactt tctttctctc tgacattcat
    39721 gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg
    39781 ttctggttca tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat
    39841 tggctcacgt acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc
    39901 tggataaact gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc
    39961 cggatcaaca tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata
    40021 gtaggacgct agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg
    40081 gatcttttgt tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt
    40141 ttgtttatct tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa
    40201 cgtcaactga tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa
    40261 atctaccgct tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat
    40321 ccctgcgttt agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc
    40381 ataaagctct gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt
    40441 ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt
    40501 aaaacggata aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac
    40561 gccttctgcc aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt
    40621 actcttttaa tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt
    40681 caggcaattc gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt
    40741 ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat
    40801 atactaaaga caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca
    40861 tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa
    40921 gcatcgtaaa ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa
    40981 tgatcacagg tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa
    41041 aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg
    41101 gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag
    41161 aagagcaagt ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg
    41221 atcttcttgc caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt
    41281 ccgatacttc taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt
    41341 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
    41401 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
    41461 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
    41521 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
    41581 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
    41641 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
    41701 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
    41761 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
    41821 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
    41881 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
    41941 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
    42001 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
    42061 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
    42121 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
    42181 aattttcaag tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt
    42241 aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt
    42301 actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct
    42361 acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg
    42421 atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc
    42481 aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact
    42541 tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga
    42601 ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt
    42661 ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt
    42721 cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca
    42781 gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa
    42841 ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag
    42901 aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa
    42961 ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc
    43021 ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg
    43081 taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac
    43141 ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc
    43201 gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg
    43261 aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga
    43321 taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac
    43381 aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat
    43441 ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact
    43501 ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt
    43561 tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt
    43621 ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt
    43681 ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc
    43741 gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg
    43801 tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg
    43861 tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg
    43921 ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg
    43981 atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac
    44041 agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg
    44101 aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa
    44161 gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt
    44221 ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg
    44281 tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca
    44341 attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc
    44401 ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat
    44461 ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt
    44521 gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct
    44581 tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg
    44641 catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct
    44701 tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc
    44761 acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat
    44821 caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga
    44881 aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg
    44941 gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc
    45001 attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat
    45061 tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc
    45121 aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta
    45181 gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac
    45241 gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc
    45301 gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt
    45361 tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc
    45421 accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg
    45481 ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg
    45541 tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg
    45601 acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa
    45661 tttacgggta cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa
    45721 cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc
    45781 aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac
    45841 ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt
    45901 gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc
    45961 gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca
    46021 gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa
    46081 tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact
    46141 tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct
    46201 gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag
    46261 agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct
    46321 aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg
    46381 attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca
    46441 acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc
    46501 tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa
    46561 acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg
    46621 gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc
    46681 ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg
    46741 gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct
    46801 tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact
    46861 cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta
    46921 atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt
    46981 gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt
    47041 tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct
    47101 ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct
    47161 agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt
    47221 gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct
    47281 gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc
    47341 ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca
    47401 gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc
    47461 gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct
    47521 tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg
    47581 aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat
    47641 tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg
    47701 aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca
    47761 atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca
    47821 gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta
    47881 tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga
    47941 ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca
    48001 ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct
    48061 tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca
    48121 agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg
    48181 tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc
    48241 aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata
    48301 ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt
    48361 ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc
    48421 ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct
    48481 caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta
    48541 aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt
    48601 agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc
    48661 aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc
    48721 attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac
    48781 tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat
    48841 gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa
    48901 ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc
    48961 caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc
    49021 gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg
    49081 cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt
    49141 agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc
    49201 aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc
    49261 taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc
    49321 aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa
    49381 gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag
    49441 aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag
    49501 aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat
    49561 atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt
    49621 aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa
    49681 aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg
    49741 catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt
    49801 ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa
    49861 gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg
    49921 ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc
    49981 cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc
    50041 aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg
    50101 aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc
    50161 aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat
    50221 ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc
    50281 gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc
    50341 atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa
    50401 aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc
    50461 taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc
    50521 tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac
    50581 acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac
    50641 ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta
    50701 tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc
    50761 gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac
    50821 ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc
    50881 tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg
    50941 ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact
    51001 tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt
    51061 tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac
    51121 aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca
    51181 ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga
    51241 cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg
    51301 atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg
    51361 atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt
    51421 gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca
    51481 tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca
    51541 ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct
    51601 gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca
    51661 tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca
    51721 aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc
    51781 gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac
    51841 cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg
    51901 actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg
    51961 tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt
    52021 acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc
    52081 ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc
    52141 gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct
    52201 gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat
    52261 aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt
    52321 aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac
    52381 gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat
    52441 cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat
    52501 atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat
    52561 atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga
    52621 acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca
    52681 atgattttag caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa
    52741 gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg
    52801 caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg
    52861 ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag
    52921 acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga
    52981 cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc
    53041 ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc
    53101 gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg
    53161 cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat
    53221 tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat
    53281 ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc
    53341 gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag
    53401 tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata
    53461 tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc
    53521 gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt
    53581 tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc
    53641 gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat
    53701 atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata
    53761 tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc
    53821 aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta
    53881 cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg
    53941 cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg
    54001 gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg
    54061 taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta
    54121 tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct
    54181 ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca
    54241 aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct
    54301 tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc
    54361 tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga
    54421 atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa
    54481 aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat
    54541 aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa
    54601 atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg
    54661 ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca
    54721 taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca
    54781 attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa
    54841 cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt
    54901 ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa
    54961 aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct
    55021 gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat
    55081 tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa
    55141 tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt
    55201 ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca
    55261 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
    55321 ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc
    55381 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg
    55441 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga tttcttattc
    55501 ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac
    55561 aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc
    55621 aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac
    55681 gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc
    55741 ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc
    55801 tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt
    55861 cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc
    55921 ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc
    55981 atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg
    56041 aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt
    56101 ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac
    56161 cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac
    56221 agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac
    56281 taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca
    56341 gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa
    56401 tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa
    56461 gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt
    56521 cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag
    56581 acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc
    56641 ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc
    56701 tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg
    56761 tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata
    56821 ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga
    56881 agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca
    56941 agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga
    57001 tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc
    57061 ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata
    57121 aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa
    57181 aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg
    57241 ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa
    57301 gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta
    57361 ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg
    57421 aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac
    57481 tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt
    57541 tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa
    57601 gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt
    57661 ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag
    57721 catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca
    57781 atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca
    57841 ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac
    57901 gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt
    57961 ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat
    58021 ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc
    58081 agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat
    58141 ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc
    58201 taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc
    58261 tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt
    58321 cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat
    58381 ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg
    58441 agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga
    58501 accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac
    58561 cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct
    58621 gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt
    58681 tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt
    58741 ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt
    58801 ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt
    58861 ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc
    58921 caattccttt tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc
    58981 ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa
    59041 aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc
    59101 tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat
    59161 caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct
    59221 taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa
    59281 ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc
    59341 accggctatt agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat
    59401 acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct
    59461 gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac tcctatttca
    59521 tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg
    59581 catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata
    59641 gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg
    59701 tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat
    59761 cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa
    59821 gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt
    59881 gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa aataataact
    59941 ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca
    60001 ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct
    60061 aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa
    60121 ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa
    60181 gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc
    60241 attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct
    60301 tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt
    60361 gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca
    60421 tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc
    60481 attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc
    60541 taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc
    60601 tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc
    60661 tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc
    60721 caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact
    60781 gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact
    60841 tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata
    60901 taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc
    60961 aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta
    61021 ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg
    61081 gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat
    61141 agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt
    61201 ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa
    61261 gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct
    61321 gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta
    61381 ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc
    61441 ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg
    61501 atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga
    61561 ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg
    61621 tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc
    61681 gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt
    61741 tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata
    61801 catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac
    61861 attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt
    61921 agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg
    61981 ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg
    62041 atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg
    62101 catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc
    62161 agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga
    62221 tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa
    62281 cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa
    62341 ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt
    62401 gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg
    62461 tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt
    62521 agctgactgc aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt
    62581 ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag
    62641 atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg
    62701 aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc
    62761 atatttaaat aacatttgtt tctttttaat aaatttgctt tttctgcata aaaagacgta
    62821 aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta
    62881 acaagtaatt ctctctggat attatccgtt atggattata cttcctgtgt cgtcggacgg
    62941 ataatcatgt tgctgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa
    63001 gctatatctt cagatgttaa tccccattct ttttgcgctt catgaagttc aagagcagcc
    63061 tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta
    63121 gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca
    63181 aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca
    63241 gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca
    63301 tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca
    63361 actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac
    63421 ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt
    63481 tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg
    63541 cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg
    63601 attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc
    63661 tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt
    63721 caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg
    63781 tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt
    63841 acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc
    63901 tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg
    63961 acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc
    64021 acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa
    64081 aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt
    64141 ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt
    64201 tccgcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg
    64261 acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa
    64321 cggacggctt atgatgtcta atacttataa aacatactta actattcctt ttaactatac
    64381 ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat
    64441 tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg
    64501 caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc
    64561 actcgaggct cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga
    64621 aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc
    64681 tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc
    64741 cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca
    64801 gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg
    64861 acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg
    64921 atacatcgga atcatccttt agcaaagcaa acagtcatcc atccgcagga actgaaaact
    64981 gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt
    65041 ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg
    65101 atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg
    65161 acggaaaaag tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg
    65221 aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa
    65281 tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa taagttaagc
    65341 aaaagatcca atgattaaat aacataagtt tcttaatttg ttccttcctt ttatttctga
    65401 taattccagt aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat
    65461 aaaattgatt acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa
    65521 atctctgagg aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa
    65581 tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca
    65641 aaaagatctt taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta
    65701 ttggaaaagg gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg
    65761 aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga
    65821 ttgatgaacg ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga
    65881 tggaaaagtt gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg
    65941 agaacattaa taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt
    66001 acagtgatga attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga
    66061 ttccatatac ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg
    66121 gtcggaacaa taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt
    66181 tagtggaatc agaattggaa acagatatcc attcagaacc attttcactg aattattcaa
    66241 gactggtgaa tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt
    66301 tagacacgga aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta
    66361 aaaaaatccg tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg
    66421 gttacttggc catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca
    66481 atcatgagga ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact
    66541 tccagttgcg gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc
    66601 ttatagtaac gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca
    66661 attacctttg ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc
    66721 tgctgccttg gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc
    66781 agtagctact ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag
    66841 taacgtattt attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca
    66901 cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt
    66961 ggctgccttt gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta
    67021 ctctgcttta gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct
    67081 ttatggcttc ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt
    67141 gttctggttt gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac
    67201 aaaaggcgta acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc
    67261 agcaggagca tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc
    67321 cttgatgtta gcagcagctt ttgcttcatt ctttactggt gtaacagaac cacttgaatt
    67381 ctcatttatg ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc
    67441 tttagcagtg agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt
    67501 cgattacttc ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca
    67561 aggattagta acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca
    67621 tctgatgact cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa
    67681 ttcagacaat aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga
    67741 caatgtaact tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg
    67801 cctgatcgat caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg
    67861 aaaaaatgcg caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa
    67921 actacatggt ggtgctgcaa cacgacctgc acaaacaaac accgtggtta aaacagaaac
    67981 atttgccggt ggaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc
    68041 aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt
    68101 attaccagaa aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc
    68161 aacgaaacat gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg
    68221 catcaacact gttgacttaa aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa
    68281 agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa
    68341 aaacacggat atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga
    68401 acccgcacgt gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc
    68461 catcgtcttc cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt
    68521 ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc
    68581 agccaaacgt aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag
    68641 aacagctttt ttacaacctc tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt
    68701 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttcat actcaaaact
    68761 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    68821 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    68881 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    68941 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    69001 tttttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    69061 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    69121 tattcacgtt tcaataccca acctgttttt ccatttcctg cagcataaac gaattgacca
    69181 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    69241 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    69301 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    69361 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    69421 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    69481 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    69541 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    69601 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    69661 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    69721 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    69781 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    69841 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    69901 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    69961 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    70021 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    70081 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    70141 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    70201 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    70261 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    70321 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    70381 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    70441 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    70501 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    70561 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    70621 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgattaca
    70681 acaagatacg ttccttttgg gaatgggatg attcgggtag tgtttggaac atcctgactc
    70741 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    70801 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    70861 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    70921 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    70981 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    71041 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    71101 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    71161 gcttcgacta actcataatc tgaagaagga agccattctg catagatttt cccccaagta
    71221 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    71281 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    71341 tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa atccataaga aacgttgatt
    71401 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    71461 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    71521 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    71581 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    71641 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    71701 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctaggag atcgcgatta
    71761 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    71821 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    71881 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    71941 tttatcttcc tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    72001 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    72061 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    72121 aataaatttt ggggaagaca cattctaagt aaacaaagcg gtaaaaatat cttttattcc
    72181 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    72241 gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt
    72301 atttatttct aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc
    72361 atctttaaaa cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga
    72421 atgaattttt gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt
    72481 tcttaaacgt ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga
    72541 ataattggct tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc
    72601 acggttttgt ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca
    72661 cggaagatta taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc
    72721 tatatgccat agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag
    72781 aacgtccaat aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg
    72841 ctagttcctc tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta
    72901 tcgaacgctt caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa
    72961 ctatcatcca taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt
    73021 tcatcatgga gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg
    73081 cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt
    73141 gggagcctga ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag
    73201 aattattagc agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc
    73261 aagtcgatca tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag
    73321 actttcggga aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa
    73381 cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat
    73441 ccacaagaaa tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa
    73501 gaagacttaa cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat
    73561 aagtcatgac aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta
    73621 tgatactttt ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc
    73681 tgacaacaac tttcaggtgt gtcgaaccac tctaatcttt aaagataaat attaaaataa
    73741 cgattaataa tattaacagt ctttattttt caccaccaaa aacctatctt tgcatatact
    73801 aaatagacgt atatacacag ataggtttgt cttgtgaaaa ataatgatta tccttaccaa
    73861 aaaatgtttg tttttctaca tgttcgtact tatcaaaaaa tatgatcctt attgaaatta
    73921 ctggctattt atattccaac gacttcttgt gttaattttt gttatctcat agtaaattat
    73981 gtttcatttt cacttaattt agttgtcacg tgttcgtcaa ttcgtaaaaa cggtaaatat
    74041 ataaacacac ttgcggctat aattaatatt tgtaaaacag ctgctcgcca gtctccggct
    74101 gttgctaaaa atcccgatat aactggtggc gtagtccaag gaatttgcac tactacatga
    74161 ttaattaatc ccacagcagt tgcaaaatat gctactgtca aagaaaaaat cggtgcaagc
    74221 acaaagggaa taatcagact gatattaaat acaataggca atccaaaaat aataggttcg
    74281 cttatattga atatagcggg agttaccgat aatttagcaa agtccttata atcttttcta
    74341 cgactgaaga taaatattgc aatcaataaa gcaatcgtat ttcccgatcc acccattacc
    74401 gcaaaggtat ctttaaaggc catattgata atatgtggaa tttgctcttt gtttgcataa
    74461 gctgccatat tttcttgcat attctgaatc aaaaacggat ctaacaatga acctgatatt
    74521 actgcttgat ggataccaaa tccaaaaaat agattagcaa tagatgtaat taacaaaaat
    74581 cctggtaaac cagttgtaat tttacttaat ggctctgtaa ctatcgtggt aattagctta
    74641 tttatatcca tattaaacaa ttgaatgatt ataaatgaca ataaagcaaa cgaggtaatc
    74701 gtaataatga ttgggattaa tacattaaaa gactttaaaa cagcgggagg aatatttccg
    74761 gatagctgaa tctgtagttt cttattatta gaaagtttaa taaataattc tgtagccgct
    74821 aatccaacaa atatacctac aaacattcct gaagctcctg tataggatat aggtatcaca
    74881 cccgtcactt caacagcttt atccgcattt tcaggagtaa acatcatctg cataggtgtt
    74941 aatacaacaa atgtcgaaat agcaactaat accgctgcaa tatgatttgt ataatttctg
    75001 cttgcacaca tatgataaga tactgctgct acaactaata atgtagttat gcttaatgta
    75061 ccattggcaa ttgaagtacc aatttgttgt aacgtggtca atgtactagt atcgacaatt
    75121 tcccccatga atcctgttgg ttctaagatc actgaattaa taagtgtcac aaatcctgct
    75181 aacatcataa atggcataaa tgtagcaaac gcatctcgca aagttcttaa atggatctga
    75241 ttccccaact tagtcgcaac tataccaaat ttttcaaata caatgtcctt cctgctactt
    75301 ttgcccatat tttttctcct ttatgcttat tgtaatcgat tactttttcc acccaaataa
    75361 tacatcactt atcaaaaaaa gtaaatactt ttaattaaat atatatatct tttttagtat
    75421 aaaaaaataa gctctgaaaa aataattttc ccgagcttat ttttcaacaa aaacagcttc
    75481 aaaaacaaag tcttgatata aatgtctcga taccgaatac tcgaaaggtt taccagacga
    75541 tagagcaaca actttgtcta tctcaatcat aaattcacct ggatctattt ttaatatttt
    75601 tgcgtcaaat tcattcgcag tatctccttt gatacgtatg tgcgaagtct ttatttccag
    75661 acccagttca tttgtgatat aagcatatac agatttcttt aaatgttttg gttccaagcc
    75721 tggtatgact gctaatggca tgaatgtttg ctcaatcgag tatggttgac aatcaataaa
    75781 acggcttcgt acaatattat atacatagtc ttttacactg atacttaatt tatcagctac
    75841 ttttttcgat ggaagttcaa ttgagaaagt atggatttct gaatctacgt gttcttcacc
    75901 aaccacgcta aacaaacctt cgtttggacc aagaactcgt gagttatcaa ttggtgttct
    75961 gacgacttca gtcccatacc cacttctact tcgcaaaact ccttcggaaa caagtaagtc
    76021 tacgcctttc tttactgtta acttgcttgt atgataaata actgccaatt catctcctgt
    76081 aggaagtttc tcaccaattt tatactctcc atttacaatt tttttcttta tatctaagta
    76141 aatttccata tacttcgcaa tttttttcaa tggtgaccct ccaatcaaaa acatatcttc
    76201 atctatactt taacataaat aaaccgataa aatccacatc aaaattatac acacaataaa
    76261 aaagatatat atatttaaac aaaactattt actttaagtt gtaagcggtg ttatcattaa
    76321 gtggaaacag gaggtaaaaa aattatgaca actatgaaaa agctaacttt tccagatgat
    76381 ttttggtggg gttccgcttg gtcagctgag caagcagaag gaaaaggaga aacaggaaaa
    76441 gcagagacta tctgggatcg ctggtttaaa gaacaacctt accggtttta tcaacgaatc
    76501 gattcctcta ttacaaccga tcacatccat caatataaag aagatataga actaatgaaa
    76561 aaaactgggc ataactcctt tcgcgtttct atttcttggg cacgaatgtt tccaaatggc
    76621 ggtaccggaa aagtaaatcc caaagccatt tctttctatc gaaaattatt ttccgaaatg
    76681 aataataacg gaatcaaagt atttgccaac ttatatcatt ttgacatgcc tgctactctt
    76741 caagataaag gaggatggga atcacgtgaa gtagttgatg cttatgttca ttacgctgaa
    76801 acttgtttta aagaatttgg tgacttagtt tgtcattggt ttaccttcaa tgaaccactt
    76861 gggcctattt taggtactta tcttgaagat tttcattatc caaacctcat tgactttaaa
    76921 cgcggagcgc aagcagcatt cttcactata cttgcacacg caaaagcaat acaagtattt
    76981 aaagaactag ctcttcctag taaaataggc gttattctaa atttgagtcc tacttttcct
    77041 cgtagtcaaa atcctgctga tgtgaaagca gcagaaatcg ctgatttatt ttatacgcgg
    77101 agctttttag atcctatggt taaagggaat ttttctaaaa agcttatcct gttattacaa
    77161 gaatataatc agcttcctga agattatacg aaagaagatc ttgtatgtat ctacgaaaat
    77221 accgctcaaa ttcttggact aaactattac gagccacgtc gcgtcaaatc tcgttcttct
    77281 atggtggata catctagtcc atttttacca gagtggtttt tcgaaccaca tatcatgcct
    77341 ggacgacgaa tgaacgaata ccggggatgg gaaatttacg aaaaaggaat ttatgatttg
    77401 tgcatggata tcaagaacca ttatggaaac atcgaagcat ttatctcgga gaacggaatg
    77461 ggcgttgcgg atgaagaacg tttcatggat gatacagggc aaataattga tgattatcgg
    77521 attgagtata tcaaagatca tcttgcttac ttatggaagg caataaatga aggatgtaac
    77581 attaaggggt atcatctttg gacatttatt gattgctggt catggatcaa cgcctataaa
    77641 aaccgatatg ggctggtttc actcgacctt actactcaaa aaagaacaat taaaaaaagt
    77701 ggcgaatttt acaagcaatt aagtgataca aatggattta gatacgatgt agacttgtta
    77761 gttgaataat attctaaaat tccacttgaa gtgaacacat tagaaaacaa gaataatgag
    77821 aaataccaat aacaaatact ttttcttcat acttcattta tacttgtcag tataagagac
    77881 tgtgacaaat gtagtgatcc tcagaagtta ggtctggaaa tctaacttga gaagattttt
    77941 ttaaactacg tccagtcttt tttatttgtt taaaattgaa gttgacaagt cttcattaaa
    78001 aatttagaat attcacaagt ttgacgacaa ttatgaaaat ttatgtcgtg ctaaaggagt
    78061 tttgttattg gatgaaaagt tagatataaa aactgcgatc aaagacacgt tacctaccgt
    78121 ttttggttat atcggtattg gacttgcatt tggtatcgtt gggaaagctg ccgggtttca
    78181 tccgctggtc gtcacgttga tgtccctgct gatctatgct ggttctgccc aatttatcac
    78241 ggtcagcatg cttgctagcc atagtccact gctctccatc gttctctcga cctttctagt
    78301 caattcccga atgattctga tgagtatgac gattgctcct tatttcaaga aaaatcggct
    78361 gcttcaaaat ctgctgattg gcacattgct gacagatgaa agtttcgcac tgggaatgaa
    78421 taaattgaac tatactggac aaaagttaaa ctttcgttgg atgaatacag ctaattggat
    78481 ttcttatttg acgtgggtgg gttcttctct tgttggcgca cttttaggga attttattac
    78541 tgatccgaaa aagtttggtt tggatttcgc catcgtcgct atgtttatcg ggttacttta
    78601 tcttcaagtc atttctgata gaaacacctc taaacgatta cagttgatcc tgattggtct
    78661 cactttgatt ctggtatatg tcggactgat ttttatccca agtaacttag tgattttagt
    78721 tgtaacactg attgggtgcg gattgggggt gtggatcaaa catgccttct tttgagttta
    78781 cattgctgac tattttaggt tgtactttag caacttggct ttctaggatc ctaccatttg
    78841 tcttactgaa aaaattcgat ttgccgcaac cgttgcttga gtatttaagt tttgtcccga
    78901 tcgtcattat gtctgcttta tggttcagca gtctatttac ccaaaatatc ggacatctgc
    78961 cgcagatcaa tctcgaaaat gctttggctt cagttcctac acttttagct gctattcttt
    79021 caaaaagctt attggtgatc gtcttggtag gaattctttc cctttcgttg attcgcttat
    79081 tataaataaa aaaacaagag cttatggcaa attactgtca tgcgctcttg tttttatcaa
    79141 agtcaaataa gaaaaaaatc ggcgtattct tgcttatccg ttgaacggac cactaacaac
    79201 gacttttcct aatatatgtc ctgtttctac gtctttggtt gcttgtttca agttcttcgc
    79261 attcatccca tctgaataga ccttgctcaa tgtactgcgg ataatccctt catttgctaa
    79321 agcagcaatg tgcgctaaag cttctccttg cgtttcgatt tcatagttat agtctgtttt
    79381 agcgaacata tactcccagt cgaagctgat agattggttt ttccaaacac ctatatctaa
    79441 cggctctttg atccctacga tcgttcccac atgtccaaat ggtcggacaa ggtgtttgat
    79501 ttgatcaaag tagcgggtaa tatcgaataa tacagcgaca tactctactt gatcgattcc
    79561 taattcacga aaacttttac ttaagtcgtg atgataatcg attggatagt ctacacctgt
    79621 ttttttcagc cattcaaaat tcttcggact tgcagtcgcg tagacttcta ggccagccca
    79681 atgcgctagt tgattcaaaa tcgaaccgac gcccccagct ccgttgatca ctaagatttt
    79741 tttcccgcgg ttggcatttt cttctggagt cagaccaaat ttttcaaata acaattcata
    79801 agcggtcaat gaggtcaacg gcaaggctgc ggcttcttca tctgaaagat tttttggtgc
    79861 aagagcgaca atacgttcgt caaccagttg gtattcttga ttgcttcctg ctcttgtcgt
    79921 tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt tgcacttcat cacctacagc
    79981 aacaactttc ccgactccat caaaccctaa gatacgtaaa gcatttctta tcccgttatc
    80041 ttggcgaagt ttcgtatcga caggattaac agatactgcg ttgactttta ccagaagatc
    80101 ccgtgaactg ggtacaggta tctgttcttt ttcatcaata aagctttttg gatcatcgat
    80161 cgacaaccct tcataaaaac cgactacctt cattaattcc ttttctgaca tacgaattcc
    80221 tccttagctt catcaagttg gagcatccat acagttttca tttcaatata tatagtatgc
    80281 tatttgttcc acttttattc atcataatta ttctcttact taaagtaaac tatttgcact
    80341 tttttggtta aaaaagaggt tgcgacaaga cttttgtcac aacctctttt aaacggtgtt
    80401 caatcaactg accctcggta ttcgctcaaa aaacgacaaa atatgaggag ctatttttgt
    80461 ttggctgttc ttattacttg atgcaggaca gctttttcac gacctctaat tcacggtgtt
    80521 caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtgtctaag
    80581 atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct
    80641 cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg
    80701 atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct
    80761 ttttctgtat gttccctcgg ttctttttca aatgcataag gattcttgaa aatcccccgg
    80821 ccaatcatga tgccatctac gccatatttt tcggccaatt cttctcctgt ctggcgatct
    80881 gggatatctc cgttgatcgt gatcagtgtt tgcggagcga tttggtcacg taaagcaatg
    80941 attttaggga tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa
    81001 tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca
    81061 gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagcc
    81121 gcttcaatca attgtgccgc tacttctggg cgaaggatca agccactgcc ttttccacgt
    81181 tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct
    81241 gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc
    81301 atcggctgtt catcttctgt aaaggtcaat cttcctcgta cactgtcttt tccatctgga
    81361 tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc
    81421 tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa
    81481 ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca
    81541 agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga
    81601 agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaagactg tgacaaaaat
    81661 cttatcacaa ttccttttcc ggaataaatg gtgttcaaaa gcttgcatct gcggtaataa
    81721 gtccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    81781 cagaacagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    81841 tagctcaaca aacgacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag
    81901 gtcaaaacgc tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc
    81961 aaataccaag taacggcgta caaaacaacg atgaatagca caagtacgat catcgatgtt
    82021 gctactggca catcgattgt tccaaggaat ccgtaacgga aaccggagat catataaacg
    82081 atcggattga ctttcgaaac agcttgccaa aatggcggca acatagagat ggcatagaaa
    82141 acgccaccta gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcgtca
    82201 taagactgag caaaaatccc gttgagcagt cctgctaacg aaaacaagat tgccgtcatc
    82261 aataatgtaa tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa
    82321 atgatcgtta cgagagagcc gacgagaata ctgcgaccca ctccgccaat cacaaagccc
    82381 caaatgatca catgcgtagg aacaggcgct accaagattt cttcgatgtt tttctggaat
    82441 ttttgagaga agaaggacga agaaacgttg gcatacgaac tagtgatagc agacatcata
    82501 atcaaaccag gcacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgacca
    82561 ccgatcattt ttccgaaaat aacaaagtac aaggatgtcg tgatcacagg cggaaccagt
    82621 gtttggaccc agatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca
    82681 gtaaaataaa gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa
    82741 aaacagttct tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct
    82801 tgtcagctga tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt
    82861 ctgttcatct tcaaatgtac aagcgtatcc ttcaatcaca ggtttggtct catatggcgc
    82921 taaatcaaag ataaatgttt caaattgcag tttggataga agctctttca tactagtatt
    82981 ctcgatcagt tcacctgatt ggatgatccc gatgttgcga caaagcattt cagcttcttc
    83041 taaataatgc gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc ggagaaactc
    83101 ccacatctcg cgtctcaatt caatatcgac tcctgctgtt ggttcatcca agatcagcaa
    83161 acgtggttca tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag
    83221 catacgtgca cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct
    83281 gcgtttcatc gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg
    83341 ctggaccgtt tcaaagggat tgaaattgaa ctcttgcggg actaatccaa tctgttgctt
    83401 tgctcggact agatccgtat ccagatcata accgaagaca ttcacttttc cagatgtttt
    83461 attgactaaa gaagtaatga ttccgatcgt tgtagacttc ccagctccat ttggtcctaa
    83521 tagcgcatag aaatctcctt ctttcacgga aagatcgatt cctcgcaatg cttcgacccc
    83581 acttccataa acctttttca aattatttat ttcaagcgca tttgccattt atttcctatc
    83641 tccttataga catgattctt tattgtatca tggaatacag cagatgaaaa aacaacttgg
    83701 ttccagcgaa aaatcaggtt gctgggctca ggaaaatctc ctaatgaaag acatcaatcg
    83761 gacgaagtca gacaaccttt tcagaaaaaa caaatcgaag ataaaggatt actattcata
    83821 tgtgcaacaa aaggagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca
    83881 cgtataaacg gcggaagcaa aagccaagat ttttgatcca ataaatactt tcgatcactt
    83941 cgcctatccc aatacatgcc actgccgctt ttcatcaaat aacgcggctt ccgtttttgc
    84001 ggtttctact aaagtctctg ggtaatccaa aactttcagc aaggcaatcg catttcgggt
    84061 ccttgaaggt ccttctttta atttgaaatc aaaactgatt ccttttcctt ctgttacttg
    84121 ttcttcaaag tggacattct cacaataatt ctttaaaatc tctgtcaatt cgatatcatg
    84181 tgtggcgaca aatgctaaag atggatactc cgcaagccac cgaacgactg aagcggaaga
    84241 agcaatccgt tcgatcgtat ttgtcccttt taaaatctca tctacgaagc agtagcagcg
    84301 ttctttggaa gccacttggt ccaacaaacg tttgatcgat ttgatttctg aaacaaaata
    84361 actgtcgcct tcaaataaat catcttcgat tgccatggaa gtcaacacat gtccaggttg
    84421 cagggtaaac tgttctgcaa tcgctgtttg gatcgtctgt gctagaatac agctgatggc
    84481 tatacttttg acatacgtag attttcctga tgcattggaa cccgtgacta atgtattctt
    84541 ctgccagttg accggattca ctaccgcttc ttttaacaat ggatgataga tatctttcgc
    84601 tgtcacaccg ccctcagtaa actctggtcg gcaagtgatg ggcatataag tacggtaatt
    84661 caatattgca gcggctactt ctaattctcc cagcaattcc cacagcttga tggcttcagt
    84721 tgaatgcttt tctaatttag tgagaacgaa atgataggag ataaatggca gcatgaacat
    84781 gatgttgaca tattcaaaaa tcaactctgc ttcactccca ctcttcatac gaaatgaaaa
    84841 acctaattta gggatgtctc ttaatggttt agtttgctgt ttcagctctt cttgaagtgg
    84901 tgtcggcagt tttgctagct gattggcggc tgaaatcgtt gaaaccaaat aactcatgct
    84961 attcagctct gtttctagtg tttgtttctt cactagataa tagaccgtat tgaatactaa
    85021 cccaccgatt gccaaataga ttcctagtac ttgtcctaaa agaagcaaac ccaaacccac
    85081 cagcggaagc aagccaagaa aaataaataa acctacgcgt ccaatctttt tactttttcc
    85141 atccgctaga tattgttttg caaaattatg atcttttttt cctaaacgag cgaattgata
    85201 ttggattttt tctctcagtt gaggattttc ctgataaaaa gcaatcagtt tttctaactg
    85261 ctgatcttca ccaaaatcaa agctgcgtaa acgttgatac aacgcttctg aacctacact
    85321 agaataggta ctgttgattc cttcgaatac ttcgaacata tccaggtcat accaagtaag
    85381 atcatcgatt tcgctatccc atgaacggaa cttcttctcc gtgagccatg cttccttcaa
    85441 gctttcttct ttatcaaatc tcggttgata aggaattttt tcccatttgc tacgaactgt
    85501 ttcctttaat ttcaaacgtg aaaaaatgtc taaagcaact aataaaataa cagctgcaat
    85561 aataattaaa acgataaatt ctctttgacc catatccctc tccgttcctt tatttgttag
    85621 ctttagtata gcactgttgc gctatttgaa aagagggtaa aaaaacacct ttcaaaataa
    85681 aaggtgtttt tttcttgtat attacatatt tatctctcat ttatcaaaat tgacaaataa
    85741 tcatagggag gaattgttgt taattcttgt ttattctcca gtgcattgac aactgtccca
    85801 agggaaagga tagcctcagg tactattttt tcttgttctg ttggattagc taagcgaaga
    85861 acaaatcctt gttcatgaaa ataagaagga taaatcgatg atacaattaa gtcttcaggt
    85921 aattcaatca atgaaaattt acgaggaaca tcaagttcat tttgtaatgg ccaaatcttg
    85981 ttatctaacc tgtgaataaa tacattcagt ttctgctttt gataagaaat agattgttct
    86041 aaccgttttt ctgcctgttt tgccactttg aactcattaa atcttccttc ttctacatta
    86101 aatccaaaag tgactttata ctcacctatc tcttgagcta gcggtgtttc catcatgaca
    86161 tgcccttcat ttgtcgtatc tcctgaagct cgcccaggtc gccaagcaag attaggtttt
    86221 ccaagttccc ctgttgttga aaataaggtt atgattaatt gattttctat acgttcatac
    86281 tcttttaaac catctgcata aaacgtaaaa taatgatcat tttctgccac agagacactc
    86341 ttatcgaata tttctaaatt aacaggtttc tctaccatag tttctgacca attcggagga
    86401 atgacatcag gggtattttc aataaatccg ttttgaattt gtgcaattga tttttcagat
    86461 ttatctaaaa cattaattgc taagcgcata cgatgcgagt aaacagtatt atgaattttg
    86521 agtgtgcctt caacgatttg actttctccc agactgaacg atatttcata agatatttct
    86581 tccatttcgg gtttttcaga taaacgatct tttaaatctt taggaagttg agcttttccg
    86641 taaacaacta atgtttcttg taaagaatct ttatatactt tagctgtttc taattttaga
    86701 gatagttctg tatctccttc taatggtgaa taatcatacg tatcgccatc atttgctgaa
    86761 tcgatcaaat gaacgaaatc aagatattgt ctatctccaa ctatcaaatt caattgccca
    86821 tctttaaaag aaagtttata tttattgtta cttatctcag ttttgtctat ctcgattgct
    86881 gtttctaatt tttcattatt tttctcaaat tcaacaacag tgtatcctag tgctggcaca
    86941 tttacattta tttttatgtc aagttcataa taagctggtt catcaaaata ttcgaaacct
    87001 gtagcaatca tccgataagc atttttccgt tctggaaaat aaacttcttt ttcaatgata
    87061 ttatagggac tacctttgaa gtgtattttc tttgaatcag ctactacatg cacgattttc
    87121 gatccactat aattctcagt cgttgtgttg aaaacaagga cttcattcga ttttagcttt
    87181 aatgcatcag ccatccgttt tacaataagg ttttcgattc cgtcagctat ctcattagct
    87241 tctttgattc gatggaaaat atcttccgca acattatctg aaacacagcc accaatacta
    87301 tcatgagcct ggttttgcaa aatctttttc cataaccgac gaagtaggcc tttactgata
    87361 tggataccat ttctttctgc aatcagcatg agcggctcaa ctcgttttag aattttttgt
    87421 tccaatttaa aattttctat tttcattttt gttcttactg agccaataga acgatgaaca
    87481 cgagagtaaa ccggctgacg aagttcacct ttatattcag gtaattcttt tttgtttctg
    87541 ataacttcta cataagaagg ataatctgaa ataatattgt ggaatttact gtatttattc
    87601 aacttctcta cgacttgatc aaaatttaaa atcattgact tttgatctac acccgaaggt
    87661 actaaaatat ctttgttttt tccttgcata tgaagcattt caactgcttc atccaacttt
    87721 tcttttgcaa attcaggaat atctttaatt gcgtctaagg tcatcattcc agtcatataa
    87781 ccaaatggaa aattcatggc ataaacaaat ttatctccca atcctttcca gataaaataa
    87841 ggagatggaa ccatcgtttc aaagtttaat cctcgccaac acatgaaatt atcgatttct
    87901 acttgatgca aaagtgtagg caaattcgca ttgaacccaa acgtgtcagg caaataccct
    87961 accatcattg gatctccata tttaaggatc gtatcactga ttccaatcat taagtttctt
    88021 aaaattgatt cagcatccac taataaggca tctgtttgtg cataccacgg tcccacgaat
    88081 agttgtcctg ttttcatcag tcgttggata accttaagct tttgcggatt gatctccaaa
    88141 tactcatcga cgattgaagt ttgtccatct aaacaaaagt ttactttagg gttcctttcc
    88201 aattcatcta gaacttcagt aaagacctga tcagacaata cgatggcatc ttcatttgta
    88261 aaataccatt ctctgtccca gtgcgtgtgt tgaattatat gtgcatttac catatcagat
    88321 tctcctactt tccttcttct actgaattta cttgttgttc ttttttatac ttgatggatt
    88381 ttagtgcatt gaccattaac gctgtaacca ctcccccaat aacaattgat actaaatata
    88441 aaggtatatt cgtaacaaaa ggaattgcca taaaggttga aagcattgtt tcttgagtaa
    88501 caccaaaaaa tccatttaga ccacatgcta ctgcagtgcc caccacgatg cttggtatta
    88561 ctcgtaaagg atctttcacg gcaaacggga tcgtcccttc agtaatcccc acaaggccca
    88621 taatcatagc tgtctttgca ttatctcgtt ccgattcatc ataaagtttt ttgtttaaaa
    88681 ttgttgctaa aaacattcca aatggcggaa tagcaattcc taataacatg gctgtactag
    88741 gtccaaagct tccgcttgaa aaagaagcaa ccccaaaagc atatgcgatt ttattgatag
    88801 gtcctcccat atcaaacgcc atcattcctc ctaaaatagc cgacatcaaa gcagcatttg
    88861 ctcctgacat gccttctagc caattagtca gtcctgttgt cattgcttga ataggcgtag
    88921 ctagtacata ccataatatg attccaacaa ctaatgtagt aacaaaagga atcagcataa
    88981 ttgattttag actaataact tgcatcggta cttttacttt tttcaatgca ttagctaaat
    89041 agccacaagc taatccagca accattcctc caataaatcc cataccaaga ttttgacaca
    89101 tcatcccagt gataaaagct ggtgctagac cgggacggtc agcaattgaa tatgcgatat
    89161 aggctgaaat catcggtacg ataaactgta atcccgtctg accaatcatt cgcaggactc
    89221 cccagaatgt atccgtctgg tcccaaacac ctgtgccacc aaaaatgact gcaatagaag
    89281 ttagtaaccc tgctgctacg acaagcggga tcatatagga aaccccactt tggaaatgat
    89341 ttagaatttc tcttccttgt ttcttgaatt ccattagtaa ctcccccacg atatattttt
    89401 tatttgttaa ttaatgcaat tgcttcatca atcgtctctt caccatcttt tactacctct
    89461 gaggtcccta ctgttaaaat cggtttaccg tcaaagcgac ctcgttctga aacatttgta
    89521 tctactgcaa atatgacaac atctgcctcg gaaacatccg aaattgaaag tttgttttct
    89581 atccccatag ccccctgtgt ttctacttta atatctaacc ctttcttctt cgcaaacttt
    89641 tctaaattag cttttgccat ataagtatgg gcaatccctg cgatacatgc tgttactgct
    89701 actatttttg tcatatgaaa ttcctccttt taaaatgata gagcttttac caattctttt
    89761 ttatttctcg aatcttttat tcttttaaca aactcgtcat ccatcaatct tccagataat
    89821 tctgctaaaa tacgtaaatg attagtacct ttatcagtgt cttttattgc taacaaaaag
    89881 acgagatcaa ctggttgatc atctaatgcg ccccattcaa cagaccgctt taacttggct
    89941 acagccactg ttgattcaat tactgctagg cttttgccat gaggaatggc gatatttttc
    90001 ccaattcctg tggtcatatg gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg
    90061 tcagacacaa acccattagt ctctaacaat ttcgataatt tttcgactac acttttttta
    90121 tcagatgcat ttaaatctat cactatatta tttatattga ttttttcact gatcatttat
    90181 aagccttctt tcaatatata aactaatttt tcaaacgacg aactcgtttt taaatttgct
    90241 aataactcat gattcgaagc gatttcattg aatgtttcat aaattttatt caattcaggt
    90301 tttacttttt cattaagtgc aaaaaagaaa acagaatgaa caagtgtccc atcccaatca
    90361 attccttctg gattaacgta gacacagatt actggctttt ttataaaatc tacttcgata
    90421 tgcggcatag caaaatcatt catcgctgtt gaagatagtt tttctctttt gattgctccc
    90481 tctagtatcc cttctttcgc gtaattttct tttaccaatt ttttggtaat tatatttaaa
    90541 acgttcactt gattatcctt tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa
    90601 tttagaaaag catcttctgt ttgttcattt aactgtagat actgttttac ttttctgaca
    90661 tctgccccta acatcaaagg actaacaaca ataacaggaa aatttgtatt ttcaatcgga
    90721 atcgtgctta taacaagttc atcagtgact tttctatctt gtagttcatt gatgcttagt
    90781 acatcttcta tctcaaccat atctgcaaaa ccgttattta ttctttgctc taataatttt
    90841 gatgttccat atccgctact acaaactaat atcactttcg ttttgtcagg tttgtaacga
    90901 tccaaaagtg attgaatatg gagtgttacg taagcgattt catcttcatt aaaatgtata
    90961 cagaatctct cttcgatttc ctctaataaa tctacactta tcataaatga ccttttaaaa
    91021 gaatatttga ttttgtccgt ataagggtta tagatattta ctcccaattt gagtctttta
    91081 actgctgcat tgagatgtaa tactagatta ttgatcaatt cttcatcagg ataaaattca
    91141 tttaatttaa ttcttactaa ctcttgtagc tcaacaccca tatctaaata ggaaagcttc
    91201 gtttgatcta agtcgtgatt catagcattt ttttcaatcg aagtaatgtg aatgtcaaca
    91261 taagcttgtt catctattgg cacaataatt gaaaatgctt tttcaatcat tttgattaaa
    91321 ataagtgtat tctttgagat ttcttcttca gaattattac tacgtgataa gaaattgctt
    91381 gtttcaatcc tttctatcgc aatagcaagg tggatagcta aagtttgaaa ttcataatct
    91441 gtaaatttca attgtgtctt attcatgtat cgattaagca attcggctac ttttttcaat
    91501 atgtccttat ttaagaaatt attcgcttca ccaatgaaat tgatattcaa gtctaagaga
    91561 tctgttttac tgttagatgc tgtaaagcca ccccaataat agttcacaac ttctgacatc
    91621 aaccttcttt tacttttttc atcagctttg atgcgcacgc catttcgatc tgaaactaag
    91681 gacatcccat gcttgtttag tctttttcta acatctttca ttgtgctttc taccgtcgac
    91741 ctgctcacgt ataattcgtc agcaagattt tgaatcttta gagaatcatt agtattcaat
    91801 aatttagcaa acacataagt tagacggtcc tccggagagt ctgcgatatt gattttatga
    91861 ctggataata aatttcgaag tgtttctttt tttccaataa gcgaaacgcc ttttttaggt
    91921 tgaaccaaca tttgtacatc tggcattttt tggattattt ctttcaaaat taaaatatct
    91981 ttgctaattg ttttcgtaga tacatataat tgttctgata attctttgta agttgtgtgc
    92041 tcattttcaa tcaaaaagta tagtattttc atttgccgtt cgctaaattc gatgcccatt
    92101 atcttcacct cctgaataca gcttaagtgt acaattatta tctctatttt catacagaag
    92161 aaagttcttg attaatgtag aagtttctaa tattcttgat tatttcaaat caataaaaaa
    92221 gcctgcaata tttatatcac aggctttttt ctctattaaa tatatataag ataatacatt
    92281 tttctactag ttctcactaa tcttgtataa ttaaaccctc cgcaacttga cggagatgga
    92341 gaagatgccc ccgtctaatc tagcggtcat ctctcctccc attccttcca ccaatttttt
    92401 tgacaggtaa agcccgattc cgctagattc cgtatttgtg cggctggtat tttcggtata
    92461 aaaacgctgg aagacttttt caacagaggt ttgcgttcct ggtttgatag gattggccac
    92521 tgttaatcga atgaatggat cctcttttga aaatgtcacc tttgcttgtc catctgcata
    92581 acgcaataaa ttctgtatga tattttgcag cactcgcagc aacatgagcg gatctgctaa
    92641 tacagttgta tttatcgagt gttcagcgaa cgctacctcg atatttttct cagaaaattg
    92701 ttcataaaaa gagaaaaata cttcttccac ctctttagaa aaagaaagcg tatggagatc
    92761 catttctttt tgcttcgtct ctattcgagc aagatcataa aactgatccg tcatactgag
    92821 taatcttgca acagattgtt tgatgatcgt taaatacttc tcccgtttct ccgtatcctc
    92881 tacctgttcc agtaattgta cataaccgtt aatagcagtc aaaggagttc tgagatcatg
    92941 agaaaggccg acgatcgata gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt
    93001 ttgttcattt tcatccaacc atgtattcag tgcatctact aggtccatta gattcttttc
    93061 tcgaaattcg actgaaagac gaccaccata ttcttgtcgt tcagaaagag tggcgatttt
    93121 cttagttaat ctcttgattt ccctttgcag gaagaaaata aaacttccca taaagataac
    93181 aacgagaagc aacagccatt taatcaaatc taaaccccac tccccacacc gtatgaatat
    93241 acacagcatg tgcctttgct agcttccctc gaagattgga aatatgcata ttcactgttt
    93301 tctcactatc aaaatatggt tcttgccaaa ctgtttcata aagatttgct cgcgaaaata
    93361 cttttttcgg atgttctaaa aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga
    93421 cagccacacc attcacattt acatccctcg actctttgtc cagtaagatt tccttgtaat
    93481 agatgcgttg cgacagatct tttacttgga cagaagtctg atgacgaagt tggatgccga
    93541 ttcgtaacaa aagttcttcg atatcaaatg gtttcgtcac atagtcatcc gctcctgctg
    93601 ccaacaagga aactttatct ctttgatcgc tttttgctga aagaacgatg atcggaacat
    93661 tcgatgtctg tcggatttct gctaacaatg cttcacctga aagccctgga agcatgagat
    93721 ctagaaggat caaatcgaca ggatatgtgc ttaacaataa ttttccttcc gtacctgaat
    93781 aagcatcaag aagtttgtat ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt
    93841 catcgtcttc gataatcaaa atagtttcca gcaggaccac ctacttaatg tcagattttt
    93901 ggaaaataaa cattcctaga gctgatgata ccacgattgt tactagactg accagaatca
    93961 ttttcggtgc ttgcgaataa gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt
    94021 ccgtaaacca ctccgttaga aattccaagc ctttgatttt tgcgaaaaca ccgggaatga
    94081 aggccgttcc catgatataa agaaaagaaa gaagcaaact gacactggta cttctagtga
    94141 tgaatgtcaa agccactgga acagataatg tccctaatgt cagcagaagc aatacaggaa
    94201 atacttttag aacataattt ccatagtttt tgattccctc aacttcacca aatccgaaag
    94261 caagtgtgta agcaagcatg gctagtcccg tgatcaaaac cacgccaagt gttgtgatca
    94321 aaagcgtaac gagaagtttc gacacataat aagtcattcg attccgtcct aaagataacg
    94381 tattgcgaat cgtaccgttt tgaaattctt ccccaatata aaagcacgca aatgctgcga
    94441 aagccagaag aatccatgca gccatttttt ctaccattaa aatccctgtt gcaccgttga
    94501 tgactgtatc cgttgcttgg tttaattcat tgccgtattt tgtatgcata taccattcgt
    94561 tccaagaata aaaaagcgga agtacgacca tcagtcctag tgagacccac attttacggc
    94621 tcttccatac ttttattttt tcagcagtta atagattaaa catgtggatt gcctccagtt
    94681 aattgtaaga aataatcttc cagtttttgc ccaactaggt gaataccatc cacaggaact
    94741 tttgcttcta ccaatgcttg attgatggct gctacttggt caattccttc aaaaagctgg
    94801 ataacacggc tagattgtac aaaataatcc ctataaccaa gagagtcgag aaccgttact
    94861 gcttgttccg tcgccgaagt gtcaattttt ataaattgac gagattcttg tgccaattct
    94921 tctttcgata attctttgat caagtgtcct tcatgcaaaa taccatagtg agtggcgatc
    94981 tgtgacaatt catctagcaa gtggctagaa agcaagacgg tgatgccccg ttcttttacc
    95041 aaacgttgga tgatttctcg catttctgcc atccctgctg gatccatccc attcgtcggt
    95101 tcatccaaaa tcaaaaattc tggattcgtg atcagtgcat ttgcaatagc taaacgctgg
    95161 cgcataccta aagagaaatt ctttgctttc tttttccctg tattttccaa tcgcatcatt
    95221 ttcagcaggt cttcgatttc tctatccgac acaccgccgt tcgctgcttg gatacgtagg
    95281 ttatctcttg cagttaattc tggataaaga gcaggggttt caatcgattg acccatacgt
    95341 cgtcttgctg cctgcaaatc cgtcgttcca aataattcga tcgaaccttc atcaatcgag
    95401 atcaaaccca taatagttct cataaaggta gacttacccg ctccattttc cccaatcaat
    95461 ccatagatca ttccacgttt gatttcgatt gatactttat ctaatgcttt ggcagcaccg
    95521 tattttttgg ttataccagt tgctttcaag accgtttcat ttccaaaatc catacttatc
    95581 cttctttctt attgatgagt gaagtatagt tgacgggtat aaagaaagca gccagaaatg
    95641 tataaagaaa gtgtaaattt tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt
    95701 ctttcctatt atcaggcagt ttgttatacg cttgtatcag tttcctgatt ctttcttttc
    95761 gaccaaagtc gtttctctgt attcctcttc actgaaaatt tttgcttctt tcagatcaag
    95821 cttgagtaat tttaccagac cggttataag gataactata gcaagactcc ctcccagaaa
    95881 aattagactg tctcgcagac gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag
    95941 tgctaatcca agtggactgg ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc
    96001 ttgtatatgc gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat
    96061 taatccttaa aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct
    96121 accaaataag aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt
    96181 tctcctgcca actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt
    96241 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa
    96301 ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta
    96361 tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt
    96421 gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt
    96481 acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga
    96541 agctttacgg gctttcttaa gacctggttt tttcttcgta tcattgaata agtttgaaaa
    96601 accttgattt ataaaggttt tcccgtaaat atattttatt atatttgatt aaatttgaaa
    96661 tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt ttagattaaa
    96721 tttttaggct cattcattct caaatccatt ctattcagtc aataaaaatt ttaaaaagac
    96781 tttataaaca aagatgttca agcgagcatc ttattttttt gtgcttaatt tgaaaggaga
    96841 acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt tgagtttggc
    96901 tgttcaagca attgttggag caattgctcc tacgattact tttgctgatg aaattaccca
    96961 tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg aaggaacttt
    97021 tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt acaacggctt
    97081 taaacaagat attttctgta ttgaaccagg agtcccgatt tacaatgagt tcactcctgg
    97141 ctatgagaaa aacccgttac cagatatgtc cgataaagcc aaattagttt ccgttttatg
    97201 gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga tctggcaaga
    97261 agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg taaatatcgc
    97321 agccattgag gcaaaaataa atcaagccat tgacgattat cagaagaaac caaccttcca
    97381 taatagtacg gctaaaacag tattagggca atcgactact gtgacggata cgaataatct
    97441 gaatttgtca gagttcgatg aggttgtgga aaatgccgca aacattgatt atcgtgtgaa
    97501 tggtaatcag ttgataatca ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct
    97561 taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc aacagacttt
    97621 aatggccggg gcgattgata aacctaatac ctacacaatc aaaattgatg tggaaacgga
    97681 gggttcttta aagatcaaaa aagtcgataa agaatcgggt gatgttgtac caggaacggt
    97741 tttccattta gactttggaa aaactttacc tgcaaaagac gtgactactg acaaagaagg
    97801 cattgctaca ttggatgaga ttcctcatgg tacaaaagta accattaccg aaaaatcagt
    97861 accggcgcct tatacgattg ataccacccc catgactacc accattaaag caggcgaaac
    97921 catttacgtg acttcaaaaa acgcacggga aaaagggcaa atcattctgg ataaaagcgg
    97981 agttgaaaca gggagtgatc tttggaatga caattattcc ttagctggta atacgtttgc
    98041 cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg
    98101 tcatgcggaa acaccaaaag agattgctaa tgcgttggaa ttgggaacct attatgtgac
    98161 cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa
    98221 gtatgccaat caaaccgtag cccctgttac cagtaacgta aaagggcaaa atcaagaagt
    98281 cactggcgaa accactttga cgaaagaaga caaagatact ggggataaag cacaaggtaa
    98341 ggcagtcttt gagggtgctg aatacacctt gtttactgct aaggatggaa aagctgttaa
    98401 atggagtgag gcttttaaac cagagttagc gaagggaacg aaaggttctg atgaaacggt
    98461 cactttgact ttagacgaaa agaaccaagt tgccgttaaa catttagcta tcaacgagta
    98521 ttattggcaa gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa agtatcctgt
    98581 atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg attacccgaa atgttacggc
    98641 aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat cagctgcggg
    98701 cactaccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg aagggaccaa
    98761 tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat tagggtttga
    98821 tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaagc
    98881 cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta aggaaaacaa
    98941 agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc aaaaacaacc
    99001 aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt ctgttagttt
    99061 gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt ctcttgcgac
    99121 ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag ttgataaatt
    99181 gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt
    99241 ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga
    99301 aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc agcataaatt
    99361 aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt atgaaaataa
    99421 ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga ataaccaagc
    99481 ccaaactgtc agttgtacag tggaacacca tgtttctatc caaacaaagg cccacctaga
    99541 aaacggttct cagaccttta ctcatggtga tgtggtggat atgtttgatg atgtgtcgat
    99601 tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt atgctttatt
    99661 accggatggt acaaacaaag aaatttggaa atctggcaag attgattatg aagtaaatga
    99721 taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat atccagaagg
    99781 aaccaagttt acttttgcgg aaatcaatta cgataaagat gggaatatca atggaaaaca
    99841 caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc caaccatact
    99901 aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag aatctagtcc
    99961 cacagtgaag accttccctc aaaccgggga gaaaaattcc aatgttttac tgttcattgg
   100021 ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga actaaggtga
   100081 tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt
   100141 cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg gaattgaaat
   100201 ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccggt gaaggagaaa
   100261 ttataacaga aggatatgga cggaatacta cggtgattgg tcgcagttat catttgtatt
   100321 ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg
   100381 attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc aaaggctatg
   100441 agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta gtgaaagcat
   100501 aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt ttaacatttg
   100561 ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacaa
   100621 ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa gggcgaatga
   100681 ttcaagttag tatcccagct aatgtaccct tacgcgagtt tgcttatgat gcagaggtag
   100741 agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa ggaactaccg
   100801 ttaattggtt cattaaggcg gatgatttag tcttgaagcg gcagccgaat caaggaaatc
   100861 ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat gttgaacata
   100921 ttttagattg tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag
   100981 cattaacctc tgatgaaaaa atgtttctga ttatacatca aggtttactt ggagaaaata
   101041 gcgacagtga agaagactct gatgtactag actggttagg agaagaagag tctttcttta
   101101 tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga atccattttg gaagaaattg
   101161 gtgtggagat gccggatgct attgaaggct ttttaaatca attggttgaa acgaaacaac
   101221 tcccctcctc gtagcagtaa atgactagca taactttttg aaattgccta atgaaatcct
   101281 attgggcttt ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc
   101341 gcaggagatc agcacttggt ttatcacttt gttcttggct ggctactcgc tctgttcatc
   101401 ggttggatga gcgtctttta ttttcaagag tttagacaat ttgatatttc taagctatca
   101461 ctttctacta ttgaaatcgt ttggtctata aaagatttag tatgtctgct tggaagtctc
   101521 gctttttcag gagctatgat attgctttat attcactttt ttcttgatca ttggcgaagc
   101581 ttgtggcatc gacaaaagct agctcggatg attctagaga accattggta tgaagtgaaa
   101641 caaactcaaa gtgaaggctt tttcaaagat ttgaatagta gccgaaccag agagactatc
   101701 agttacttcc ccaaaatctg ttatcgaatg aaagatggat tactgtctat tcgcgttcaa
   101761 atttcacttg ggaaatacca agatcagctt ttaaaattgg aaaagaagtt agaaagcgga
   101821 ctgtattgtg agttagtaga aaaggaactc aaagactcct atgtggagta tactttactg
   101881 tatgatatga tcgccaatcg aattgggata gacgaagtag tggcagaaaa tgggactctg
   101941 cgattgatga aaaatcaagt atgggcttat gattctttac cccatatgtt aatcgctggt
   102001 ggcacgggtg gagggaagac ctatttcctc ctcaccatta tcgaagcact attgaagtca
   102061 gatgcagaat tgtttatcct cgatcccaaa aatgcagatt tagctgattt aggtacggtg
   102121 atgcctcatg tttattcaca aaaggaagaa atttctgctt gtgtggaaga tttctatgaa
   102181 cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac aggagagaat
   102241 tatgcctatc ttggacttcc acccaacttt ttaatctttg atgaatacgt ggcttatatg
   102301 gagatgttaa cgacgaaaga aagtgcggtg attttgaata agctcaaaca aatcgtgatg
   102361 ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat
   102421 ttaggagacg gaatccgcga tcaattcaac tttcgcgtgg ctttgggtcg tatgagtgaa
   102481 ctcggatatt ccatgatgtt tggggaggtc gaaaaaaact tctttatgaa gcacatcaaa
   102541 ggtcgaggtt atgtggatac aggtggcagt gtgattagtg agttttatac gccacttgta
   102601 ccaaaagggt atgatttctt agattcaatt agtaaattag aaaagatgaa tgattgagtg
   102661 atcttcaata ctgactttat acttcaagta acgtataata cttttatata gtaggaagaa
   102721 aggggataat taatatggaa agtaatgatt cttatttaga gtatgcaaaa gcaaataagg
   102781 ataagtttat ttcttatatt atcaaaggga aatcccccga agttggacga gacgcagttt
   102841 ttatggcagg gagtcctggg gctggaaaat cagaggttgc cattggatta gctgagaatt
   102901 atgataacca tgtgattatt gatgcggatt actttcgcac ccagtttcct gattataatg
   102961 gaaaaaactc tagtctcttt caaaaagcat cttcttggtt ggtagagcag gcatttagat
   103021 atgtcttaga tcatggctat tcttttattc tggatgcaac ttttgcaata ttaagtgctg
   103081 aaaaaaatat ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc tatgtttatc
   103141 aagaccctaa aatcgcttgg aaatttacac aagaacgtga aattgcagag ggacgtcatg
   103201 tgccgaaaaa ggcatttatt aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa
   103261 agagtaaaca tccagatgta ttgcttcata tcataataaa agattatacg aataatattt
   103321 ccgaagttca ttatgctacg gataatctag aattattttt accactaggg tatagtagga
   103381 aagatttgga ggttcaatta catgactgag aaagaacaag taacgaaaat tgtaaagaaa
   103441 tacaataaga gcattgcgga tttatcggaa aatgctactg caaaagagtt caaaacggtg
   103501 attaaatatg tagccgatca ggccaatgaa aaacaacgga aattagttgg attaaataaa
   103561 aaatagtagg acaaattaat aatgtatgag ccaagagcaa aatgttcttg gcttttttct
   103621 ttgcaatgaa tgaggtaatg tatgagattt ttagatttat tcgccggtat tggtggcttt
   103681 cgtttaggta tggaacaggc gagccatcat tgtatcggtt tttgtgaaat tgataaattt
   103741 gctagacgta gttataaagc aattcatgat acaagtaagg aggtggaaat gcatgacatc
   103801 acaagtgtat cagatgagtt tattcaatcc ctcggaccag tggacatcct ttgcggcgga
   103861 tttccgtgcc aagctttttc aattgcggga aagcggcaaa gattttccga tactcgaggt
   103921 actttattct ttgaaatcgc tcggttcacc actcttctcc gacctaagct tttattcctt
   103981 gagaacgtcc gaggattgct caatcacgaa ggaggggcta cgttcgagac gatcctccga
   104041 acgctggatg gattggggta tgacgtggag tggtaggtgc ttaactcaaa ggcctacatt
   104101 ccccaaagcc gggagcggat attccttatc ggacattctc gagacgcatg taccgaacaa
   104161 gtatttccta tcattggacc gtcttcaaca tctgatcaaa acatcagaaa tttggtaaat
   104221 atcaatcctt ccaatcgagg aatgggtgga caagtctatg gttcagatgg cgtagcgcca
   104281 accttaacag gtgatgaagg aatcaaaatc gcactgccag caagggatgg aatctctgta
   104341 gcaggactat taccgggaaa gtttgaacaa ggtaatcgag tttatgaagt tactggaacc
   104401 gcccccacct tgtcaacgaa acaaggtggt acaaaaatca tgattcgaaa aaatgaaaca
   104461 gaatatcaag aggtgaagcc agaagacagt gtgaatctgg cttttcctaa ttccacaagt
   104521 cgccgaggtc gtctgggaaa acagagtgtt cataccttac taacaggaga ccaacaagcg
   104581 atcgtcacgg atcagtatca aattcggaag ctaacaccta gagagtgttg gcgacttcag
   104641 ggctttcccg attgggcttt tgatcgagat tctcaagtca attcagatag tcagttgtat
   104701 aagcaagccg gaaactctgt aacagttccg gtcatttttg atattgccag aagattaaaa
   104761 gagttggatg tacatggttt atgatgagtt gattggagaa atttattggg tgattggaaa
   104821 gattcagtca gacccagaat tagaagaaca attgcgacgg ttaaaatttg atattcgcaa
   104881 gaatggtgtc aaagtcgctg gcgatccata tttaatgaat gaggagacgg atgctcgaat
   104941 tgaaataaat caagtaattg cagaatttga gcggattgcg gaccaagcaa aagaaccaga
   105001 tattcgtcaa tacttgttag aaatgaaagc agagttggaa gtaaatggta ttacagatga
   105061 gtaacctaaa ccaacacaac tgactgttag gcgctgaccg cggaggattc gcgaagcaga
   105121 aatcctccgt ggtgctgtgc ctgttcatgg acggcgttag ccgaccatga tgtctcaccc
   105181 ccgtatctaa caggggggta caaaaacaca aaaaataagt aagcaaccat taagaatgct
   105241 tgttgtatca aagatagtaa gtaatttgta taggtgttaa ctcatttgaa aaagttaaca
   105301 ccttgaattt ggaggtgaga acttggcaca acggaattta gattatcggc tattaaaaga
   105361 ccgacgcaac gaatacggcg tttcacaaaa taaattggct acggcttgtg gacttagtcg
   105421 gccgtactta aatcagatag aaaatggtgg cgtaaccgca tctataaaaa ccatgaggaa
   105481 aatttttgac caactggaaa gctttaatcc cgatttgcct ttgacgttac tgtttgacta
   105541 tgtaaggatt cgctttccca caacggatgc acgaaaaatt attcaagaaa ttctccattt
   105601 gaaatttgag tatatgctcc atgaagatta tgccttttac tcctatcaag aacagtatgt
   105661 catgggtgat attgtggtga tgttgtctca tgaagaagat aaaggtgtgc ttttagaatt
   105721 aaaggggcga ggttgccgac aatttgaaac ttttttactc gctcaaaagc gtagctggta
   105781 cgactttttc gaggattgtc taaaggccgg tggcgtgatg aaacgcttgg acttggcaat
   105841 caatgatcga gtggggctat tggatatacc tgagctaacg aaaaaatgtc agaaggaaga
   105901 atgtatctcc ttgtttcgta cctttaaaag ttatcgttcc ggggaacttt taaaggctga
   105961 tgaaaaggac ggcatgggca ataccttata tatcggcagt ctcaagagtg aggtttattt
   106021 ctgtttgtat gaaaaagact atgaacaata tatcaagtta gggattccat tagaccaaac
   106081 tgagacgaaa aatcgttttg agattcgact gaaaaatgac cgtgcttttc atgcgattca
   106141 agatttgtta aaaggtcgta gcattgagag caccacgttt tccattatca atcgttactt
   106201 gcgatttgcg gataaagtag aaggtaaaag gcgaaccaac tggcccttga atgaacaatg
   106261 ggggcgtttt attggacgga atcggaaaga gattcaatta acttccgagc caaaaccgta
   106321 taccattgaa cgaaccttga attggctagg ccgacaagtc gcccctacgt ggaaaatggc
   106381 taaggagttg gatcgattga atcggacaac ctatatccag gatatggtac agaatgcacg
   106441 cttatcagac cgacataaaa agattttgga gcaacaaagt atggcaattg aaaatttgat
   106501 tatatgaaaa gaggaatcgt catgggaatt gtaaaagata tttttttggt atttggtggc
   106561 agtatgcttg gtgttactgt gatgtgcttg atgaatgcaa gtgcagcagc ggatcgagca
   106621 atggaaaaga aaggaaagtg acaagatgaa tttcggacaa aatttatgtg cgactcgttc
   106681 cttagtgaaa agctaaggca ctaataaaag aacgaaaaag tttggttatt aggagaactg
   106741 ataatctgac aggtggaatg atgaagtaac gctctgaaac gcctgccctg agcctccgac
   106801 aagcattgta tgaggaaact cagaatgttt gaagctcggt aaagacggct gaaagatacc
   106861 gtaatagttg atgaacatat atcttctatc gaaaactatc cagaggtggg tagcgtatcc
   106921 aacaggtcgg gggtctataa aatatctatg gttagaatgt tctataagga actgacgaaa
   106981 cagcgaatgt acgggtctaa atttgaactt tgtagaaatg caaaggtacg agaaatcgta
   107041 gctggtgtgg caaagtaaga atataggtta tgaaatgcca tataacgtta caggcggtat
   107101 caagccagca ggcttatagc tagtgaccta aggatatata tgtatagata agattatcgg
   107161 aacgaggaaa ggtattgggt tctcatcaag agaataaact gacgaagaac aataagcagt
   107221 tgaactaatg ctgaaaagca gagggcgaac ctaagaaatt cttgtaatga gaatggagga
   107281 atgccctcaa gtcgtgtata tattagaaaa gtattatgtt gtttcaataa ggattacgag
   107341 taagataaaa aagctcactc aatttcaagt aaaggagtga agccttatgc ctaaaaataa
   107401 aaatgaaaca ttgtgtgtag aagatttaag acacgctgaa tattacgaaa tgcaaaacac
   107461 ctttgatgat ttatatgcca aaagtaagaa tggagatatt ttcactcatc ttatggatat
   107521 tatcctatcg agagaaaata tattactggc atatcgaaat atcaaagcaa atgcaggcag
   107581 taaaacagca ggaacagacg gtacaataat taaagatatt ggcaaattgc cagcagaaac
   107641 agttgtcaaa aaagtaagat acattgttgc aggcagtcca catgggtatc gacctaagcc
   107701 agtgaggcga aaagaaatac caaaaccaaa tggtaaaacc agacctctag gtattccttg
   107761 tatgtgggac aggcttatac aacaatgtat aaaacaagta ttagaaccaa tctgcgaagc
   107821 taaatttagt gaaaacagct atggttttag acctaatagg tcagttgaaa atgcaataaa
   107881 agcaacctat aaccgcctac aaatcagcca gctacattat gtgattgaat ttgatattaa
   107941 gggtttcttt gacaacgtga accattccaa gctgataaaa cagatatggg ctatgggaat
   108001 acgggacaag cacttgatat tcattttaaa aaggatatta aaagctccaa tcaaaatgac
   108061 taacggtaca ataacatatc cagagaaagg aactccgcaa gggggaataa tatccccact
   108121 actagcaaac attgttctta atgagttaga ccattgggta gaaagccaat ggcaggaaaa
   108181 tcctgttaca aaaaactatg tggttcatat caataaaagc ggtagcccat gtaaaagcaa
   108241 cgcttacaaa gaaatgaaga aaacaaagtt aaaggaaatg tatatggttc gttatgcaga
   108301 tgattttaga gttttctgcc gctacaagga aagtgctgaa aaagccaaaa tagcaatcac
   108361 tcaatggatt gaacaacgat taaaattgga agtatcacaa gaaaaaacaa gaattgtaaa
   108421 tgttagaaaa agatactccg actttcttgg tttcaagatt aaaatgatac cgagaagaaa
   108481 gaagttagtt gttaaatcac atataagtga taaacaattc aaaaaccaaa aagacaagct
   108541 ggtatcacag gcaaagaaaa ttgcttctcc ctcaaaaggg aaaacggaat tagaagaaac
   108601 aagactttat aattcaatgg tattaggtat gcaaaattat tatcagattg caacgaatgt
   108661 aaatctggat tgtatgaaac tgaatagagc tgtaatggct atcttcacta acagattagg
   108721 tacaaacaaa aggggcagat tgagaaaaac aggcagacaa cttacgacaa aggaggcaac
   108781 aagatatggt aaatctaaaa tgcttcggta tgttgctggc gagggcgaac ctattttccc
   108841 tgttggatat atccaacaca gaaaaccaat ggcaaaggtg tatttagcga attgctacac
   108901 tctagaggga agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca
   108961 gttaatgaaa ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct
   109021 attttctgct caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga
   109081 aatacattgt catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct
   109141 tattttggta aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca
   109201 aaaatatata gctctattga agctaaataa agtccagctc ataagactaa ataaatatag
   109261 agtattagct ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca
   109321 atataaacac gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg
   109381 aaaagtttga gatatttata atatcaggaa cttacctatc catatatcaa tggtttttaa
   109441 ccaatgccca atccttagtc ttactggcaa ttgtggtcat tggtttgttt ttaggattta
   109501 agcgggagtt ttccaaactg attggctttt tggtgattgc tttgattgcg gtcggattgg
   109561 tctttaatgc ttctggggtg aaggatgtct tactcagttt gtttaaccga attgtcggtg
   109621 cgtaaggggg aagcacgatg gaacagatgc gtgtttatat tgcgaacctc gggaaatata
   109681 acgaaggtga attggtgggg gcgtggttta ccccacctgt cgattttgat gaagtgaaag
   109741 aacgaattgg gttaaatgac gaatacgagg aatatgcaat tcatgattat gaacttccct
   109801 ttgagattga tgagtatacg ccaattgagg aaattaatcg gttgtgtggc ttagcggagg
   109861 aacttgaagg aacgccagtt ggtgaagttg cttctgagat tcaacatgct ttcttcaatt
   109921 cttttgagga gatggtagag catgtggatg atattatcta ttatccagat tgcgatgata
   109981 tgagtgatgt agcatatttc ttgattgtag aagctggtga ttttggagaa gtaccagaac
   110041 acttgaagac gtatattgat tttgatgcgt gtggtagaga cttggagatt ggaggcaatt
   110101 atttggtaac gagtcgaggt gtttttgagt atccatatta acaaaattat tgaagggtag
   110161 ttgatttcta aactatcctt ctttttaaag gaatgatttg atgaagaaaa taaaaagcta
   110221 caccagtatt tgggcagttg aaaaggtcat ttatgcaatt aatgactttc aactgccttt
   110281 tcctgtgact tttaaccaga tggcttggtt tgttttatcc ttgctgtttg tcatcgtatt
   110341 tgcccatgtg cctccactat ctatgataga gggggccttt ttaaaatact taggtatacc
   110401 ggtggcagtt acttggttca tgtcgcaaaa aacgtttgac ggtaagaaac cattgggatt
   110461 tttacgatct tttattagct atcacttacg atttaaggtc actttcgcag gaaagaaagt
   110521 caaagaacag aagaaacgat gggacgaacc cattactctg gtaaggagtg tgaactatgt
   110581 acccgattaa gtatattgaa aataatcttg tctttaatca agagggagaa tgttttgcct
   110641 actatgagtt aataccttac aattactcct ttttgtcacc tgaacagaaa tatcaagtcc
   110701 acgataactt tcgtcaattg attgcgcaaa atcgtgaagg caagattcat gccttacaaa
   110761 ttgccacaga aagtagtatt cgtgccacgc aagaacgatc aaaaaaggaa attaccgggc
   110821 gattaacaga agtagcgaaa caacgaattg atttacagac agaggcatta gtatccatga
   110881 ttggcgatag tcagattgat tatcgctttt ttattggctt caaactgatt gcgacagatg
   110941 aagaggtcaa tctaaagaat ctcaagaaat ctttcttctc cggctttcaa gaatttgtct
   111001 atggagtgaa tcatcatttg atgggcgatt ttgtttcctt atccaatgaa gaaattcgcc
   111061 gatataccaa gcttgaaaaa ctaatggaaa gtaaattggc tcgaagattt aaagtcagac
   111121 gagtcacacc gagtgatctg atgtatttga ttgaacatat ctatggcgaa aagggtacac
   111181 catttgaaga atatgaattt cagctaccaa agaaaaagct gaagtcggaa actttggtga
   111241 aacgttacga cttattacgt cccagtcgtt gtttgattga agaaaagccc cggtacttat
   111301 gcatggaaca tgagaatcat gaatcatatg tagcgtactt aacaattaat acaattgtgg
   111361 gggaaatgga gtttccctcc tcagaacttt tctattacca gcaacaacaa tttacattcc
   111421 caattgacac tagtatgaat gtggagattg tgacgaacaa aaaggcactt gctaccgttc
   111481 gcaataagaa aaaggaatta aaagacttgg acaaccatgc ctatcaatct gacaatgaaa
   111541 caaattccaa tgtcttagat gcattggatt ccgtagatga attggaaact accttggatc
   111601 agtccaaaga atccatgtac aaactgagct atgtggttcg agtaagtgcc gaatcagtag
   111661 atgaactaaa gcgccggtgt gatgaagtgc tagattttta tgatgatacc aatgtaaaac
   111721 tagtccggcc ttttggcgat atgatggggc tacatgaaga attcttacct tctagtaagc
   111781 gatacatgaa tgattatatc cagtatgtta cgtctgattt tctcgccgga cttggttttg
   111841 gcgccactca gatgttaggg gagttggaag gcatttactt tggttataac gtagatacgg
   111901 ggcgcaatgt ttacctgaaa ccggcattgg cctcacaagg ggtcaaggat tcagttacca
   111961 atgcgctagc agcggcattc ctcggttcac tcggtggcgg aaagtccttt agtaacaatc
   112021 tcttagttta ttacgccgtc ctttttggtg gacaagctgt gattgtcgat ccaaaagggg
   112081 aacggggtgg ctggaaagaa accttgccag aaattgcgga tgaaatcaat attctcaact
   112141 taactagtaa gccaaaaaat caaggattac ttgatcctta tgtgattatg gagaaaaaga
   112201 aggattctga aagtctagca attgatattc tgaccttttt aacaggaatt tctagtcgag
   112261 atggggagaa gtttccagtg ttgcgtaagg ccattcggac ggttacccaa agcaatcaac
   112321 ggggattatt acaagtaatt aatgagcttc gaaaagaagg ctcaccagtt gcggaaaata
   112381 tcgcggatca catcgagtcc tttaccgatt atgattttgc ccatttgctt ttctcagatg
   112441 gttcgattac ccagtcgatt agtttagata ggcagttaaa tatcattcaa gtagcggatt
   112501 tggttttacc agatgcagaa accacttttg aagaatatac caccatggaa ttgcttagtg
   112561 tttccatgct aatcgtcatt tccacttttg ccttggactt tatccatagt aatcgagaaa
   112621 tcttcaaaat tgtagactta gatgaggcgt ggagttttct tcaagtagcg caagggaaaa
   112681 cattatcgaa taagctcgtt cgtgctgggc gggctatgaa tgccggggtt tactttgtca
   112741 cgcaaaatag tgatgattta ctggatgaac gcttgaagaa taatatcggg ttgaagtttg
   112801 cgtttcgcag taccgatatt catgaaatca agaaaacctt ggaattcttt ggcttggatc
   112861 gagaagatga aagcaatcaa aaacgcctac gagatttgga aaatggccaa tgcttgatgc
   112921 aggatttgta tggtcgggta ggcgtggttc aggtacatcc agtctttgaa gaactcttcc
   112981 atgcctttga tacccgtcca cctgttcaag agagaatgga gtgaggggat gaaaaagaaa
   113041 ctttttcgga ttgtgggaat cgtttttatt agcatactca ctctgatagt tctattgagt
   113101 ttagttggta cggtagccga ggcgacaggg ctagttgatg atacagttga agcaggaaat
   113161 ctatattcca aatattcact gaacaactat caactagatt tctttgtgga tagctcttgg
   113221 gattggttgc cttggaattg gggtgatggc ttagggaaaa gcgtgatgta tggtctttat
   113281 gccattacta atttcatttg gacggtgagt ttgtatttgt cgaacgccac gggttatgtg
   113341 gttcaagagg cttacaaatt agatttcatt tcggatacgg ctgaaagtat tggaaagaat
   113401 atccaaacat tggctggtat tactgaaaat ggactccaga cctccggatt ttattttggg
   113461 tttctattat tgatgatttt agctctgggg gtgtatgtgg cgtataccgg attattaaaa
   113521 cgagaaacca cgaaagcagt tcgagccgtg attaattttg tgatgatttt tctactatcg
   113581 ggttcgttca ttgcttatgc gcctacctat atcacaaaaa tcaatgactt tagctcagat
   113641 gttagtgaag cggcccttac ccttggaacc gagatcgttg tacccaattc agaaagccaa
   113701 ggaaaagata gtgtggattt gattcgggat agtttgtttt cgattcaagt ccaacaacct
   113761 tggttactac tgcaatttga tgattccaac atagaagaaa ttggtgaaga ccgggtcaac
   113821 aagattctat ctgtgagtcc agatgaaaac aaaggcaaag atcgggaaga agcggtcaag
   113881 gcagagattg aagacaatga caatgcgaat ctcagtatta ccaagaccat gaaccgttta
   113941 gggaccgtgg tctttttggt attgttcaat attggcattt ccttctttgt ttttcttcta
   114001 acagggatta tgttgttctc gcaaattctc ttcattatct ttgcgatgtt tctgccaatt
   114061 agtttcttat tgtccatgtt gccgacttat gaaagtttag gaaagaaggc gattattcgt
   114121 ttattcaata cgattatgat gcgagcgggt gttactttgg tgattacgac tgcatttagc
   114181 atctccacga tgtttttcaa tatttcggca acctatccat ttttcatggt tgcttttctg
   114241 caaattgtga cctttgccgg tatctatttc aaattgggcg acattatgag tatgttcaac
   114301 ctccagagta atgatagcca gtcaatggga agacgcgtca tgcgaaaacc gcaaatgtta
   114361 atgaatcgaa aattgcggca actcaatcga aatgtgggac gagctttggc ttttggtggt
   114421 ggtgcagcag tcggaaataa gttggcaaag gaacaatcaa aatccaaatt taagccatcc
   114481 ggttcttctc tacgaaagaa ttcacgatta ccgaacgatc acgaaatact atctgactta
   114541 actaaggaga atccagtttc aaataataag aaacaatccc gaatgaattt ggctgggaga
   114601 aaaatcggca aagttttgga tacccaagct ttggtgaaag ataagactaa acaagtaaag
   114661 gatcaggtaa ggaatacgcc aacgaatttg aaatacaatc tccataaagg aatcgaaaag
   114721 tcgaaaaaag caccagaaga attcaagcgc ggactgttcc aagaaaaagc ggatcgagtg
   114781 gatttgcgag aaaaacaacg acaacgaaga aatgcgaaaa tggacgaaaa acgcaagata
   114841 ctgagtgaag caacaaatcg tcagggaaaa agaagggcga atattccaat agataaagca
   114901 gctcaacctc agaaagatat gatgctgaaa aaagaagcac caaagcggat tgtgagagca
   114961 aatctgaatc ccgaaatcaa acgaaaacta actagtcaag aaatcatttc aaaagggaaa
   115021 agccaatcag caagtaagaa aaattctttc caacaagtga aacagaggtc cactctgcca
   115081 aagcagacca atcaaaaagt acaaaaaaga atgaaccatc gtccaaaatc aaggtcaggt
   115141 gagaaaaaat gaacttgaag aaagtgggtg ttatttcatt aacttttctg accatctctt
   115201 ttatgggtat tttgatgtgt gtgggtttgt tgtttggtga agacagtgag ggaaatggta
   115261 gttctggcac ttctcctggc ggaactagtg tctcagaaga agtattgaaa caccgtgcaa
   115321 tggtggaaaa gtatgccaaa gaatcggata taacagagta tattccaatt cttttggcga
   115381 ttattcaagt ggaatctggt ggcacaatgg aagacgttat gcaaagttct gagtcagccg
   115441 gattaccacc taattcatta agtaccgagg catccatcaa acaaggctgt ctctattttg
   115501 cgtctttggt taagcgttcc aaagaactag ggtgtgatca agatagtatt attcaggcct
   115561 ataactatgg tggcgggtat ctagatcatg tcgggaaaaa cgggaagaag catagttttg
   115621 ctttagctga atctttttca aaggaaaaat caggtggtca aaaagtcgat taccccaacc
   115681 caatcgcgat taaggaaaat ggtgggtggc ggtataatta tggcaatatg ttttactgtt
   115741 tattagtgaa gcaataccta actacaactc agtttgatga caaaatagtc caagaaattt
   115801 ttgatgaggc atttaagtat gaaggaacgg cctatgtgtt tggtggctca agtccagaaa
   115861 cggggtttga ttgtagtggc ttgacccaat ggtgttatgc caaagttgac ctgaaacttc
   115921 ctcgggtggc tcaggatcaa tatgatgcca tgactcatat tgatctaaaa gatgccaaac
   115981 caggtgattt agttttcttt cattcaacct atgatgccgg tacttatgtg acccatgtgg
   116041 gcatttacgc aggggagaat cgcatgtttc atgcaggaga tccagtgggg tggacgaatc
   116101 taacggaaaa ctattggcgg cagcatctta tcggagctgg ccgatacaaa taagagaaaa
   116161 gaggaaaaag aatgaagata aaaattgaac ggaatcaaaa ggaaaagagc ccaaaaaaga
   116221 aaaaagaacg agttgtatcc gtgggtaagc atcgaaaaat ggtgcttgcc ctttggcttt
   116281 tattgtcttg tagtcttgct tttggcgttt ataaaaattt caccgccatt gatcaacata
   116341 caactcatga aaaagtggtt ataaaagaga aggtagtgaa tacttctggc gttgaaagtt
   116401 ttacagagga ttttgcgaaa gaatattttt catggaaaaa taacaaagaa gcaattgaaa
   116461 agagaatgag taatctcgaa cagtacctta cagaaaacgg tctcgcttta atccaagata
   116521 tggttcgagc agatattccc acgagttccg aagttcaatc tgtaaaaatt ctagatgttg
   116581 aaaaacactc ggaagaattt gtcgtttcat ttttagtgga gcaacatatt acagagggaa
   116641 aaaagacgca atcaatttct tctgcatatc gagtgacaat ctttgaagat gaaaacagga
   116701 atcatttagt aactagtttg ccaacaatga ttggaaagcc agacaaggca aaatatgaag
   116761 caaaacaagt agaaaacgat tcagaaattg atgccaaaac aacggaagaa atcaccgatt
   116821 ttttagaaac attttttaaa ctttatccat cagcttctga aaaagaattg gagtattatg
   116881 ttgaaaatgg tgcgatgcga ccaatcaata ttgacttaaa gttttcggag ttagagaatc
   116941 cagtttatct acaaaacgga aaaaattttc tagtgagtac aaagataaaa taccttgata
   117001 atgtatcaaa aaccactagc tactttcagt acaaattagt attacaaaaa aatggaaact
   117061 ggaaaatcgt tgatgttaaa taataatgac tcaatctaat tttattagaa gttataagta
   117121 gtgtaactta tttcaggcag aagtcgccca tcctctatag gtgggtgatg aatgccattc
   117181 ggtaactcga aaaccgacaa ttaagacata gtgccttttt attttaatcc atgtcataat
   117241 cagtctatat aggaaatggt tgatatacca tggaattaga tacaaataat cattcagttt
   117301 ttcttcttgg ttaccccctt attttagtag tgaaacattg taaacatgtc attgatgatg
   117361 taatgtcagc ttatgcaaaa actgcctttg aaagaatttc agaatcacat cacattacct
   117421 tagatgaatg aaatcgtgat aaagaatact ttcatattat gctcaaagca caacctaaaa
   117481 ccgaattaac aaaattcagc aatgcctaag ctcacgtctg ataaaatgag actttctaag
   117541 ggtcaaaaat ttcctttgaa tacgattatt gcttagataa aagtaaaagc cttagaacta
   117601 cggggatagc ttggttaata agagatatcg ttgttagtaa agacataggc ttgcaagtat
   117661 gctctattcc caagaatctc ccccttcaag caccaaggat cgttaaattc agctaagtgg
   117721 caagtaattc actagacgat aattatctcc atatttcaat ctcagctttt ccatttttca
   117781 tagcaaattc cgttattttt atagcatcta aaagattcag cttttccact gatctttcac
   117841 cacttttcag cttttttatt gtgcttaaat tgataccagt accttttgat attgaattgg
   117901 aagtaaaatc attaagggtt ttttttatta aattcatatc cgcaattcca gcgttcataa
   117961 gaatgaagat tctcctctca tagactactt tgaaaatggt actacagtgg ctgtttatat
   118021 atttcagtag gttactgtag tggtcatctt attataatta gaaaaggaac ctaaatcaaa
   118081 aatgctaaag aaaattttca tagtattaag cctctcatga ctaaagccac ggaattcttg
   118141 ataaaagagt atacttgcaa gcctaaacag cggtatctct tattaaccaa gctatccccg
   118201 ttgttcctaa ggttcttgat gttttctaag gcaatgcttg tattcatagc ccttggtcaa
   118261 aatattgata ctatcgttga cgtttcgatt atgatgtata cggcaaatag gacaagtcca
   118321 ttctcgaatt gtgagtgact tcttaccatc tgtgtgtcta cattctgaac caatttgact
   118381 agacggaaat catttatcta tcttaatgat tttttgacag aagtatcgaa aatctttaca
   118441 taatctgggt gatgtattct tatgcagatg agagcgttgg ctccgtattc ataaccagta
   118501 gcctctaaaa cgtagtccat cggtgggata cctagcttgc ggttgccagc ggctttcgca
   118561 gtcttcttgt gatcaaatca agcatctcat tgagcactag tattttgaag ataatacttg
   118621 agtttttcgg cttttagaag ttcacttcat cttccatcat ccatggaaat cccactcacc
   118681 catacgcagg cctccaaaaa ttcttcaaca gtggtttttt cagacatttt tctgaatacc
   118741 tccttgtttt ttgatacgcc agatagtcag gaaaaaggca acaaaacaca aaacaaagct
   118801 gcctaaaaag gtaatcttca ttccatataa aaagacatcc ggccgatttg cgatatagtt
   118861 agttacttgc tggcccatct tagcagacat cgcttgatat aagaccgtcg tagaaagcga
   118921 aatcccgctg accatcccca aatttcgggc aaaggaattc atcgagcctg ccagtcccaa
   118981 gttttcgata gtgaccgagc tcataaccat cgtattattg ggggactgga acaaggcatt
   119041 gccggcaccc atgatggcag tagcgacaat gtatagccac aaaggagagt ccactgacaa
   119101 aaacatatac atgacctggg ttaccgacaa taagcccaag cctactaata ccaaaatctc
   119161 cgggccaacc cggtcggtca ggtagccgct taaaggcgcc ccgacgacca tcatcaaagg
   119221 aaagaccatc atcaacagcc cagcatagct aggactcaac ccgcgagcat tttgcagata
   119281 aaaaggaatc actacattga caaagaagtt ggaagaaaaa atcaacaagg ctgtaatcaa
   119341 gctcatggta aaaatcttgt tcttaaaaat accgaaagaa atcaatggag aggctgcttt
   119401 cttttcaact tttaaaaagc tgacaaaggc aatcactgaa attacgaaca gtacaagggg
   119461 gactggatgg aaaaagccac tttcttgccc caaaaagatt gctccaaaaa agctgataat
   119521 cgtagccgca atggcagcaa agccccctcc atcgattgta tggccgctca tggtgatatc
   119581 ttttggtaag aatttttcag caatcaacat cgttacgatt cccaccggta cattgatcca
   119641 aaaaatgtac gcccaagaaa attgggacaa aatcaagccg ccaagacctg gtccagcaat
   119701 cgagcctaat gaaacaaacg cacctattgc tcccaatgcc cgaccacgtt catgtatggg
   119761 aaagacttcc gtgatgattc cggcatttgt agccatagtc atactagcac caatggcttg
   119821 taccacccgt gcgaacaata aaatacctag cgaatgattg aacccacaca acagcgatcc
   119881 caagacaaag atcagcgtac caatccgaaa tactttgatt ttcccacggc tatcgccaat
   119941 tttaccaaac agcaacaaac aagaacagac aatcatcaga taaacggaca ctacccattc
   120001 cgcttgattc attggtatgc ccatatcatc tgagatggta ggtagtgcaa tattgacaat
   120061 actgccatcc aatgtggaca tgaaggtaaa catagatata gcaatcaaaa tccaccagcg
   120121 atttttttgg acttttgcat cttcttgata actatacata ataggatttc ctttctaatg
   120181 atcgtgtagc taagaacaat ataacctatg tctactgccc aacaacttct cgcgactcta
   120241 gccataggaa gcttgagtaa tttcgatttg atatcctcat gtaaccatgt aagttggaga
   120301 tgctttattt gaacttactc tatagtaact agtgataatt cgctttactt tcttctagca
   120361 atttctaaaa gtcgacttca ttgttcttca gattaatagt gtgagctatc tcgtgagacg
   120421 taagcaaatt tccattctac tttttcccaa ttttttcata tttatggtgt tgcacctatt
   120481 tgttttccaa tttgcttttc aaatacattg ctatgatatt caattccata ggattatact
   120541 aaatatcaat agtatgaata atatattata gatgtttata tatcactgtt aattattatc
   120601 cacaataatt aatgattacc tcgatatttg tcaatcaaag gttgaaacaa tttattttca
   120661 gcatcttttc tagcttgagc tgcactctta atagaatcat acgatcctaa atagtacctc
   120721 ttttttttta aagttatata cgcaatctat ttctctttag aacgataaac tcccttaaca
   120781 ccacttgtat tatttttcgg taattttgac gttagacact ctattactgt cccttcaaca
   120841 catgtccttg tttcttgcct ttttaaacat ccacaacttt gagtatgtcc aaaaaataag
   120901 ttactataac ttatctcaca catactgcca cactcacaat gacaaaccca ttttgttttc
   120961 catgaacagt tattgtccaa aggatacttt gcagtaagta atccgaattt tttattttca
   121021 atgtgatgct tttttgattt caaacatcca cagcttttta catcaccttt tagaagtcgc
   121081 tttgccgata ctaacttttt tctcccacac agacattgac attcccaaac aacagttcct
   121141 tcacttcgca gtttagttgg cttgattgca actaacttac caaaagtttt atttgtcaaa
   121201 tttttatatt tatgcctatt tttataattt ccacatccac aacttttggt tctacctgat
   121261 agtaagtttg atcgatatac aatcgtttca ttcccacagt cacataggca acgatatttt
   121321 ttatatccat tatgagtaat ctcctcaaca acagtaaggt gaccatatct ttccttcact
   121381 gcacctaccc tcccaagaaa gaagtaccat ataatttcaa ttaactttta ataaatcaga
   121441 aaaaaatatt tccaattttt cttagtctga ttttatgact aatttacaat acaagactga
   121501 acgatacgta cacgaaatcc tcaagataga taaattgttt tcatctagtg aaacctgctc
   121561 aaaatatgag cataaagacg gcaataatac actcgatatt cgataatgga cttgttttat
   121621 ttgtcaaacc catcgtaatc gagatatcaa ttccagtctc aatatcctga tcaaaggtct
   121681 aagaatccaa ccattttctt gttactttgt cagattgatt acctttttta gtctccgaat
   121741 aataaggttt tcggatcaac taacatgctc tgagaaagac tactatcttt gcaaatttcc
   121801 aaatgaaggt gtgggccagt actattccca gtacttccta catagccaat tatttgtcct
   121861 tgcaaaacat tatctccagt ttttactagg tattgttgct ggtgagcata taacgcggtt
   121921 gatccatctt cgtgttgtat caccacgtaa tttccccaag agtagtgaaa ctctgcttta
   121981 acaaccttac cagctttaac tgcatgaatc ggctcaccct gtgtagctgc catatcaagt
   122041 cctcggtgaa attcatttcc acgatttcca aacaaactgg ttattgaata attatgaata
   122101 ggcacttggt atccagtctt actcatttgt ggacctttaa cttctttatc atagttggtt
   122161 aaatcgtagg ttttaattaa ttcatttagc ttcaaatagt attgtgtatc tgttgcatac
   122221 cgtcctgtaa ggaatttagt tgcttctcga tatgttttac tattagcttt taatgctcct
   122281 gcataaaaat ctttatttcc actaattccc ttagttaata atttggcata atcttcaaag
   122341 ctttcctcat agttttcata cactctaaat tttgattggg tcgtataaag cgttccatta
   122401 cccaaatctt cgtttgttac cataatgaca aagtttccat tataagttcc ttttatccca
   122461 aataaattgt aattcggtgc ctgagctaac tggctttgac cactggcaga ttctaagatc
   122521 gcttgtgcaa tcataacaga agcatataaa tcatatttct gaccaatttt tctcgcagac
   122581 tcaccaattt tcataataaa attttctaca gattcatttt tgtcaaaatg aatttcttca
   122641 ttaccagata gtattggttg tgattggaaa gattctgttg attctatttg ctgtggtgtc
   122701 gaaaaagttt ttgtttgtcc ttccgttttt ggcgcttcct tttttgaaga ttctatatgg
   122761 tttggttttg tcactttcga agaagttatt tcacttgtac tacttgaagt tgttgaagat
   122821 tcggtacttg aaatcgtact actttcactt ga
//
LOCUS       DAZKCC010000002        87873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000002 DAZKCC010000000
VERSION     DAZKCC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.002"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1208
                     /locus_tag="ACG8VH_000117"
     CDS             207..1208
                     /locus_tag="ACG8VH_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP9108715.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1227..2246
                     /locus_tag="ACG8VH_000118"
     CDS             1227..2246
                     /locus_tag="ACG8VH_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFP9108716.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2348..3064)
                     /locus_tag="ACG8VH_000119"
     CDS             complement(2348..3064)
                     /locus_tag="ACG8VH_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9108717.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3033..3818)
                     /locus_tag="ACG8VH_000120"
     CDS             complement(3033..3818)
                     /locus_tag="ACG8VH_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9108718.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3809..4558)
                     /locus_tag="ACG8VH_000121"
     CDS             complement(3809..4558)
                     /locus_tag="ACG8VH_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9108719.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4856..5380
                     /locus_tag="ACG8VH_000122"
     CDS             4856..5380
                     /locus_tag="ACG8VH_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9108720.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5396..6895
                     /locus_tag="ACG8VH_000123"
     CDS             5396..6895
                     /locus_tag="ACG8VH_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9108721.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6983..7585)
                     /locus_tag="ACG8VH_000124"
     CDS             complement(6983..7585)
                     /locus_tag="ACG8VH_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9108722.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7689..8645
                     /locus_tag="ACG8VH_000125"
     CDS             7689..8645
                     /locus_tag="ACG8VH_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP9108723.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8950..9906)
                     /locus_tag="ACG8VH_000126"
     CDS             complement(8950..9906)
                     /locus_tag="ACG8VH_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9108724.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10080..10640
                     /locus_tag="ACG8VH_000127"
     CDS             10080..10640
                     /locus_tag="ACG8VH_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108725.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10633..11457
                     /locus_tag="ACG8VH_000128"
     CDS             10633..11457
                     /locus_tag="ACG8VH_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP9108726.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11467..12084
                     /locus_tag="ACG8VH_000129"
     CDS             11467..12084
                     /locus_tag="ACG8VH_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9108727.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12102..12785
                     /locus_tag="ACG8VH_000130"
     CDS             12102..12785
                     /locus_tag="ACG8VH_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9108728.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13468..14751
                     /locus_tag="ACG8VH_000131"
     CDS             13468..14751
                     /locus_tag="ACG8VH_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9108729.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14767..15654
                     /locus_tag="ACG8VH_000132"
     CDS             14767..15654
                     /locus_tag="ACG8VH_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9108730.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15671..16507
                     /locus_tag="ACG8VH_000133"
     CDS             15671..16507
                     /locus_tag="ACG8VH_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9108731.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16510..17133
                     /locus_tag="ACG8VH_000134"
     CDS             16510..17133
                     /locus_tag="ACG8VH_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108732.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17130..18422
                     /locus_tag="ACG8VH_000135"
     CDS             17130..18422
                     /locus_tag="ACG8VH_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9108733.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18781..18878
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18994..19575
                     /locus_tag="ACG8VH_000136"
     CDS             18994..19575
                     /locus_tag="ACG8VH_000136"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9108734.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19578..20885
                     /locus_tag="ACG8VH_000137"
     CDS             19578..20885
                     /locus_tag="ACG8VH_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP9108735.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21301..21789
                     /gene="purE"
                     /locus_tag="ACG8VH_000138"
     CDS             21301..21789
                     /gene="purE"
                     /locus_tag="ACG8VH_000138"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9108736.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21782..22906
                     /gene="purK"
                     /locus_tag="ACG8VH_000139"
     CDS             21782..22906
                     /gene="purK"
                     /locus_tag="ACG8VH_000139"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9108737.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22919..24214
                     /gene="purB"
                     /locus_tag="ACG8VH_000140"
     CDS             22919..24214
                     /gene="purB"
                     /locus_tag="ACG8VH_000140"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9108738.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="ACG8VH_000141"
     CDS             complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="ACG8VH_000141"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9108739.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26293..26670)
                     /locus_tag="ACG8VH_000142"
     CDS             complement(26293..26670)
                     /locus_tag="ACG8VH_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108740.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26926..27654
                     /gene="purC"
                     /locus_tag="ACG8VH_000143"
     CDS             26926..27654
                     /gene="purC"
                     /locus_tag="ACG8VH_000143"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9108741.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27654..27908
                     /gene="purS"
                     /locus_tag="ACG8VH_000144"
     CDS             27654..27908
                     /gene="purS"
                     /locus_tag="ACG8VH_000144"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP9108742.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27910..28581
                     /gene="purQ"
                     /locus_tag="ACG8VH_000145"
     CDS             27910..28581
                     /gene="purQ"
                     /locus_tag="ACG8VH_000145"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP9108743.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28582..30804
                     /gene="purL"
                     /locus_tag="ACG8VH_000146"
     CDS             28582..30804
                     /gene="purL"
                     /locus_tag="ACG8VH_000146"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP9108744.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30789..32228
                     /gene="purF"
                     /locus_tag="ACG8VH_000147"
     CDS             30789..32228
                     /gene="purF"
                     /locus_tag="ACG8VH_000147"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9108745.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32251..33303
                     /gene="purM"
                     /locus_tag="ACG8VH_000148"
     CDS             32251..33303
                     /gene="purM"
                     /locus_tag="ACG8VH_000148"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9108746.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33300..33878
                     /gene="purN"
                     /locus_tag="ACG8VH_000149"
     CDS             33300..33878
                     /gene="purN"
                     /locus_tag="ACG8VH_000149"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9108747.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33875..35410
                     /gene="purH"
                     /locus_tag="ACG8VH_000150"
     CDS             33875..35410
                     /gene="purH"
                     /locus_tag="ACG8VH_000150"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9108748.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35426..36700
                     /gene="purD"
                     /locus_tag="ACG8VH_000151"
     CDS             35426..36700
                     /gene="purD"
                     /locus_tag="ACG8VH_000151"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9108749.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36822..37736
                     /locus_tag="ACG8VH_000152"
     CDS             36822..37736
                     /locus_tag="ACG8VH_000152"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9108750.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37869..39434)
                     /locus_tag="ACG8VH_000153"
     CDS             complement(37869..39434)
                     /locus_tag="ACG8VH_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9108751.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39427..40878)
                     /locus_tag="ACG8VH_000154"
     CDS             complement(39427..40878)
                     /locus_tag="ACG8VH_000154"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9108752.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40892..41908)
                     /locus_tag="ACG8VH_000155"
     CDS             complement(40892..41908)
                     /locus_tag="ACG8VH_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9108753.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42128..43216
                     /gene="chvE"
                     /locus_tag="ACG8VH_000156"
     CDS             42128..43216
                     /gene="chvE"
                     /locus_tag="ACG8VH_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9108754.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43270..44796
                     /gene="mmsA"
                     /locus_tag="ACG8VH_000157"
     CDS             43270..44796
                     /gene="mmsA"
                     /locus_tag="ACG8VH_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9108755.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44798..46003
                     /gene="mmsB"
                     /locus_tag="ACG8VH_000158"
     CDS             44798..46003
                     /gene="mmsB"
                     /locus_tag="ACG8VH_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP9108756.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46260..47237
                     /gene="guaC"
                     /locus_tag="ACG8VH_000159"
     CDS             46260..47237
                     /gene="guaC"
                     /locus_tag="ACG8VH_000159"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9108757.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47501..48925
                     /locus_tag="ACG8VH_000160"
     CDS             47501..48925
                     /locus_tag="ACG8VH_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9108758.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(48967..49224)
                     /locus_tag="ACG8VH_000161"
     CDS             complement(48967..49224)
                     /locus_tag="ACG8VH_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108759.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            49386..50495
                     /gene="pdhA"
                     /locus_tag="ACG8VH_000162"
     CDS             49386..50495
                     /gene="pdhA"
                     /locus_tag="ACG8VH_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP9108760.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50498..51475
                     /locus_tag="ACG8VH_000163"
     CDS             50498..51475
                     /locus_tag="ACG8VH_000163"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP9108761.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            51500..53143
                     /locus_tag="ACG8VH_000164"
     CDS             51500..53143
                     /locus_tag="ACG8VH_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9108762.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            53151..54557
                     /gene="lpdA"
                     /locus_tag="ACG8VH_000165"
     CDS             53151..54557
                     /gene="lpdA"
                     /locus_tag="ACG8VH_000165"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9108763.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            54719..54994
                     /locus_tag="ACG8VH_000166"
     CDS             54719..54994
                     /locus_tag="ACG8VH_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP9108764.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            55000..55767
                     /locus_tag="ACG8VH_000167"
     CDS             55000..55767
                     /locus_tag="ACG8VH_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP9108765.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            55935..57764
                     /gene="typA"
                     /locus_tag="ACG8VH_000168"
     CDS             55935..57764
                     /gene="typA"
                     /locus_tag="ACG8VH_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP9108766.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            58025..59797
                     /gene="recQ"
                     /locus_tag="ACG8VH_000169"
     CDS             58025..59797
                     /gene="recQ"
                     /locus_tag="ACG8VH_000169"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP9108767.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            59911..60213
                     /locus_tag="ACG8VH_000170"
     CDS             59911..60213
                     /locus_tag="ACG8VH_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP9108768.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            60216..61379
                     /locus_tag="ACG8VH_000171"
     CDS             60216..61379
                     /locus_tag="ACG8VH_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9108769.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            61405..64833
                     /locus_tag="ACG8VH_000172"
     CDS             61405..64833
                     /locus_tag="ACG8VH_000172"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP9108770.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            64854..65978
                     /locus_tag="ACG8VH_000173"
     CDS             64854..65978
                     /locus_tag="ACG8VH_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP9108771.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            65959..66405
                     /locus_tag="ACG8VH_000174"
     CDS             65959..66405
                     /locus_tag="ACG8VH_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9108772.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            66389..66727
                     /locus_tag="ACG8VH_000175"
     CDS             66389..66727
                     /locus_tag="ACG8VH_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP9108773.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            66846..67406
                     /gene="rsmD"
                     /locus_tag="ACG8VH_000176"
     CDS             66846..67406
                     /gene="rsmD"
                     /locus_tag="ACG8VH_000176"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP9108774.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            67403..67894
                     /gene="coaD"
                     /locus_tag="ACG8VH_000177"
     CDS             67403..67894
                     /gene="coaD"
                     /locus_tag="ACG8VH_000177"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9108775.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            67884..68927
                     /locus_tag="ACG8VH_000178"
     CDS             67884..68927
                     /locus_tag="ACG8VH_000178"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP9108776.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            69086..69814
                     /locus_tag="ACG8VH_000179"
     CDS             69086..69814
                     /locus_tag="ACG8VH_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9108777.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            69930..70175
                     /locus_tag="ACG8VH_000180"
     CDS             69930..70175
                     /locus_tag="ACG8VH_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9108778.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            70172..70390
                     /locus_tag="ACG8VH_000181"
     CDS             70172..70390
                     /locus_tag="ACG8VH_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9108779.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            70526..71065
                     /locus_tag="ACG8VH_000182"
     CDS             70526..71065
                     /locus_tag="ACG8VH_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP9108780.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            71058..73331
                     /locus_tag="ACG8VH_000183"
     CDS             71058..73331
                     /locus_tag="ACG8VH_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP9108781.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            73369..74415
                     /gene="holA"
                     /locus_tag="ACG8VH_000184"
     CDS             73369..74415
                     /gene="holA"
                     /locus_tag="ACG8VH_000184"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9108782.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(74504..74758)
                     /gene="rpsT"
                     /locus_tag="ACG8VH_000185"
     CDS             complement(74504..74758)
                     /gene="rpsT"
                     /locus_tag="ACG8VH_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9108783.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(75158..75427)
                     /locus_tag="ACG8VH_000186"
     CDS             complement(75158..75427)
                     /locus_tag="ACG8VH_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108784.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(75689..75817)
                     /locus_tag="ACG8VH_000187"
     CDS             complement(75689..75817)
                     /locus_tag="ACG8VH_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108785.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(75897..76352)
                     /locus_tag="ACG8VH_000188"
     CDS             complement(75897..76352)
                     /locus_tag="ACG8VH_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9108786.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(76490..78418)
                     /locus_tag="ACG8VH_000189"
     CDS             complement(76490..78418)
                     /locus_tag="ACG8VH_000189"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9108787.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(78615..79448)
                     /locus_tag="ACG8VH_000190"
     CDS             complement(78615..79448)
                     /locus_tag="ACG8VH_000190"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9108788.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(79640..80344)
                     /locus_tag="ACG8VH_000191"
     CDS             complement(79640..80344)
                     /locus_tag="ACG8VH_000191"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9108789.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(80367..80993)
                     /locus_tag="ACG8VH_000192"
     CDS             complement(80367..80993)
                     /locus_tag="ACG8VH_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9108790.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            81319..83694
                     /locus_tag="ACG8VH_000193"
     CDS             81319..83694
                     /locus_tag="ACG8VH_000193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9108791.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(83718..84209)
                     /locus_tag="ACG8VH_000194"
     CDS             complement(83718..84209)
                     /locus_tag="ACG8VH_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP9108792.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            84396..85316
                     /locus_tag="ACG8VH_000195"
     CDS             84396..85316
                     /locus_tag="ACG8VH_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9108793.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            85470..85814
                     /locus_tag="ACG8VH_000196"
     CDS             85470..85814
                     /locus_tag="ACG8VH_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP9108794.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(86004..86504)
                     /locus_tag="ACG8VH_000197"
     CDS             complement(86004..86504)
                     /locus_tag="ACG8VH_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9108795.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            87032..87115
                     /locus_tag="ACG8VH_000198"
     tRNA            87032..87115
                     /locus_tag="ACG8VH_000198"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87066..87068,aa:Leu,seq:cag)
     gene            complement(87439..>87873)
                     /locus_tag="ACG8VH_000199"
     CDS             complement(87439..>87873)
                     /locus_tag="ACG8VH_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9108796.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        28296 a        15343 c        18794 g        25440 t
ORIGIN      
        1 aatcacttta cataagtttc aaaagtttct ttaatttcct attgttcaat aagtaacaat
       61 gtaaagataa aattaaaaat gttaatacgt attagcaaaa taaaagcttt ttttattgtg
      121 aataaatgaa cataattagg aggagaaaga tgaacttatt tgacattttt cgatcgactt
      181 tgacagatat gtcgattgtc agtgcaatca cttcaacagt ttttattata ttattagggt
      241 ttgtttgtag aaaaaagggg attttttctg aatctgttgg aaaaattcta tcgaaagtcg
      301 tcttaacagt ggctttgcca gcactggcat ttcgagcttt catgcaagat atcaatacag
      361 aattactgat gcaaggaatc aatgtcttga tttggggatt agttatttat attattctta
      421 ttctagtttc aaaacctttt ttcttaagct ataaagggga taagcaaaca acgttgcgtg
      481 tcctgacgat ctttggttct acgacctttt tcggaacgcc tatcgtcagt gcgatttatg
      541 gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc tttttgtatt
      601 cttatggata cataaaaatg agtggcttga agatggaagc taaaaatatc aaagcgatgt
      661 tcgtcaatcc aatcattatc gctacttttg ccggcctttt catttggctg ttccaagggt
      721 atctgccaca agtgtcagtt acaggtgcag aaggagagat aagccaagta gcgtttttgc
      781 gaattgacca gactgcaccg tggctatttc agtcgatgga ttatttagct ggtttaagtt
      841 caccattggc gtggctagca atcggcgcaa cattaggtga agtaagcttc aaaaaagctg
      901 catctgaaaa aacttcatgg tattacagtc tagtcaaagt ggtatttgta cctgcattga
      961 atatcgtgat tctagcagtt ttgacggcaa cgaatattct accagtcaat tttgaagcac
     1021 tggcaacaat cgttatcatg atggctacac cagctgcaac agtggcagca gcctatgcta
     1081 tcagttttga tagggaggca cttttagcct ccaatgcatc cttactgtca actatttttg
     1141 ctgtggtact gacacctgta tggatcgttg tattacaaat gattcatggc ataggtttat
     1201 tttcataaga aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt cgtccgaacg
     1261 agatagattt ttttgaaaaa ttgaatattt accattatga tctcagtctt tatgaagaac
     1321 tgttgacaca tgacaatatc gtgtcagcaa aggaccatga tgctgtattg ttgaggggaa
     1381 attgtatagc agatgagacc aatcttgcaa aaatgcagga atatgggatt cgttatgttt
     1441 ttacacggac tgtgggagtc aatcatatcg atctacaagc ggcagctgat tttggtatga
     1501 cagtagcccg tgtaccatcg tattcgccaa atgcgatagc agagttatct ttgacttttg
     1561 ccatgatgct tttgagaaat actgcgtata cgacgatccg aacatctttc aaagattttc
     1621 gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg atcataggaa
     1681 caggcaggat agggttgacg gaagctgttt tgtttaaggg actaggagcc aaagtgatcg
     1741 gctacgatat tcatcagaca gaagctgcta aaaaagtatt gacgttccac tcgcttgaag
     1801 agttattagc caaaagcgat attgtcagta ttcatgtacc tcatatccca ggtgaaaacg
     1861 atcaaatgat caatgaagga tttttagctc aaatgaaaaa aggtagcatc ttgattaata
     1921 cagcccgtgg agagcttcaa gataatgaag ctattttaag agctttgaaa agcaatcatt
     1981 tagctggttt tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc cgatccttca
     2041 agccatggga gacgattcca gaccctgcta tccagcaatt agtagaaatg taccctagag
     2101 ttcttgtaac cccgcatgta ggttcaaata cagatgaggc attatcaaat atgatttcga
     2161 caagtttcga gaactttcgt gaaataatag aaactggaaa aacgaaaaat gaggtatctc
     2221 ttcctaaagc ccgtcaatta agataagaaa acatggatat tcgtcaataa aatgtttaaa
     2281 aatagtccaa gtaaacagtt gttacttctg tatacttgga ctattttgta tgggataatt
     2341 cgatgactca acagtaatct gtgagctttt tccgcccatt ctttgtcaat tcataacagg
     2401 ttagcggccg gccgatttcc aaatatttga ttctttcgtt caatatatct tgttctacta
     2461 aaaaattcaa atatttcttg acggatatac gtgacagtga gcttttttga gccagttctt
     2521 ttgttgaaaa aatagtatct tcttttagaa taaatgcact tatttttttt aaagtcagtt
     2581 ttgacagacc tttaggcaaa tcgatgaatg atggtatttc gtgaatggga gtggtgtggt
     2641 tgaatacttg atcgatcaat ttctgatcgg ctttttcagt cgtttggatt gtctgagaga
     2701 aagcaagata cttttcgatc gccagctgaa aacgttgcaa agtgaacggt ttgatcaaat
     2761 aatctaaggc accgtagcgg atcacattct ttatggtttc gatatcatct gcagcagtga
     2821 tcaaaatcac tgggatatca tttccttgct gtctaagcca agataaaaaa tggataccac
     2881 tttcgccagg catgtataca tcaagcagca gcagatctgc gtttttttgt ctgaaatatt
     2941 tttttccttc ttctacattt ctgaaacttt cgattacgtt catattaggg atgctttcca
     3001 caaaagtacg attcaattct gcaaccattg gatcatcttc gattatcagg acatttgtca
     3061 tcattttgat cttccttttt tggtaattct agatagacga ttgtaccgcc atttgtttgt
     3121 ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg aatggatgag attcaatcca
     3181 aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa atattttttc tgagatttct
     3241 tctgatatcc cgacaccgtc atcgcagacg gtcacgatga atacttcagc ttctgtgtca
     3301 tagtttaaat gaatccggac ctgtccgttt ctttcatgtt tttgtagcac ggcttctttt
     3361 gcattatcaa gtaagttccc taacacttgc aaaagtgaat ggatggagtc atccatctcc
     3421 attttaggga taaaagaatc gctttctaac agcatttcaa cagattgttc tttggcttcg
     3481 tttattttgc ctaataaaaa accggcaatt gcaggtactt tgatcagact tgagacttgg
     3541 ccaatctctt ttttataatt ttgatttagc tgctggataa aatgggtgac ttcttcatac
     3601 ttcttcaaat caatcaaacc agaaatgaca tgaagtttat tcatgaattc atgggtttga
     3661 gctctcatgg agtttatata ttgtttcgtc cccccaagct cctccattaa tttcattact
     3721 tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct aaaaagaggt
     3781 gtaacgctag aaatagtttc tatcccattt aaatacatgg attgagtccg aacggtcgtt
     3841 tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat tatcactgat ttttttccta
     3901 cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt gtattgatca
     3961 agaggatagt tttatcagaa gagatggcta agattccttc atctacttca ttttcgataa
     4021 tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc agtacttttt
     4081 tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca ataatcaatg
     4141 aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg tttgttaagc
     4201 caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa tagcggaagc
     4261 cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc acttttttca
     4321 catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga gaatatcgaa
     4381 tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg atttcatttg
     4441 ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg atgagtttat
     4501 cattggcaac gattttagca atgctagaga ttttatctga cacactagat cggttcatcg
     4561 aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat gtggagaaac
     4621 aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa aaaggaaaca
     4681 taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa atttaacaaa
     4741 ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta aaagaagcta
     4801 tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg tgtatatgat
     4861 agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg caggattggc
     4921 agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg atccgatgct
     4981 tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc gtttaacaga
     5041 tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt tggtattttt
     5101 gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt cgtcttctga
     5161 aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac gaggtatggg
     5221 agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt tttacagccc
     5281 ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca aggaaagaat
     5341 ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt gttaaatgga
     5401 aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga tgttgctgat
     5461 cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca tcagtggaag
     5521 ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca aaattgtgcg
     5581 tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca gtctattatc
     5641 gagtaaattg gacgtctcca cccctgaagt gtatacggaa caaggcgtac cggtgatgtg
     5701 tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta cagcagcaga
     5761 acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag tattagaagg
     5821 acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa atcgcgataa
     5881 gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg ggttgatcat
     5941 cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact ctttagggaa
     6001 accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag ctctaaaaga
     6061 aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat tacagcgaca
     6121 aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct acaaacaaga
     6181 acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg cccgagcaca
     6241 acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgtcaaa aacaaatcga
     6301 actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag tgaagaaaaa
     6361 agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag tcaaagaagt
     6421 gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg aagccaataa
     6481 gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa aagcagaagc
     6541 cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag ctgtcttgag
     6601 tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc taggaaatat
     6661 cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg gagcgaaccg
     6721 agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa aagcgacaac
     6781 aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta ctagccaagt
     6841 aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag aataaagtag
     6901 tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc tctccatttt
     6961 tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt tggatcttag
     7021 cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt tcgaccaaat
     7081 cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa ttgataccga
     7141 ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac aaagcggatt
     7201 cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa atgataccgg
     7261 aattattgtt aataccatct gacacgacga tcacaataat aactagaata ggaagcaaga
     7321 tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa atgatagcta
     7381 atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg gttgtagtca
     7441 agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt cctcgttgag
     7501 tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc gtacgcatac
     7561 ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt actatcatac
     7621 ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa taaaaaaggg
     7681 ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc ttcttttcga
     7741 tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc attgcattat
     7801 taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga atcgcagcct
     7861 ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat actatttttg
     7921 gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa ttaaccgaag
     7981 ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac atgtcttggc
     8041 agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct ttgcttgcaa
     8101 atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag atcgaaaaag
     8161 aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat aaagaagatg
     8221 tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa gatgccagag
     8281 aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac ttctctatga
     8341 gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga atccagcaac
     8401 cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa acattttcag
     8461 agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta ttgtatcggc
     8521 aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg ttccagtttt
     8581 tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa aaaatccaaa
     8641 aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac aacctcttct
     8701 ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa caaacatgag
     8761 aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct cacaacctct
     8821 ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa acgaaaaata
     8881 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac
     8941 cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt cgatgacatc
     9001 ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta ccatattctt
     9061 tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct ttgtaccatc
     9121 tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca cataaccgtt
     9181 ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc tgacagcagc
     9241 aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc ctgtttcaaa
     9301 aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac gagcgaaaga
     9361 tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat tgaagaaccg
     9421 gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt ttaaagcccc
     9481 cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt tgaagattgt
     9541 catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt cagccagttc
     9601 ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt ccaatgctgc
     9661 caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt cgatttcagc
     9721 atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac ggtgagcgaa
     9781 gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg tctcttgatt
     9841 gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc attgatattt
     9901 tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct attattataa
     9961 caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat gaaagcagcg
    10021 atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag gggataaacg
    10081 tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt gaaactgaaa
    10141 aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa tggaaagatg
    10201 gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc actgattatg
    10261 aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa aaacgaaacg
    10321 ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg cttagcagca
    10381 aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt agataccttg
    10441 acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg aattttcgtc
    10501 tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc gattgggtga
    10561 atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa gaacaacgaa
    10621 aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat gatcgcagca
    10681 atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa tcaagcagtc
    10741 gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac aaccagcagc
    10801 aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga tgttaaaaca
    10861 gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga aattgatgag
    10921 tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat tgctccagaa
    10981 tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat ggtctcagct
    11041 taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga tgtgatgagt
    11101 cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat tacggagcca
    11161 ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa ctggtacaac
    11221 agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc taaatggatc
    11281 gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca cgaagatata
    11341 actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa cgctgagtat
    11401 atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga aaagtaggaa
    11461 ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg ttggccagca
    11521 gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa gaatctgtct
    11581 tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca atttattgaa
    11641 cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc tagtatcatt
    11701 ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag caaatacaat
    11761 aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga aacaaaggaa
    11821 tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga ttcatgggaa
    11881 cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa tcctgcgctg
    11941 tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt gcaagatgca
    12001 ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga gacgtatcaa
    12061 ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa caatttacgc
    12121 cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc attttagaag
    12181 caagcgggat caagatattt ggaaaattga tccgttcgat catctttact acggatattg
    12241 cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt acacctcatc
    12301 cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc ggtgtaggtg
    12361 gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa gcgcaaggtt
    12421 cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa gtttgtcgag
    12481 tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata gatgaaaagt
    12541 tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca gaagcagtac
    12601 gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt ccaacggaag
    12661 aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg ccaccaagta
    12721 gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa aagaatcatg
    12781 aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc tcgtcttttg
    12841 tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt ttttttacct
    12901 gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa caaaaaaatg
    12961 tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta aaaaaggcac
    13021 ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga actgaccaat
    13081 caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat ttcatacaga
    13141 attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca tgcttattta
    13201 gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt aattatcctc
    13261 ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact atctaatttt
    13321 tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct tggtttttat
    13381 tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt atattgaaaa
    13441 ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat cttagcttta
    13501 agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa aatcacgctg
    13561 aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt aatcgagctt
    13621 tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta tgaaggatat
    13681 tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt ccaactgaac
    13741 aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt ggagcaaggg
    13801 ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta cgacgggcaa
    13861 ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa aaatatattt
    13921 gaacaattaa atatccctga accagcttca aattggacat gggatgaatt tgaacaaatc
    13981 agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga agattttata
    14041 gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta taatcgggaa
    14101 gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt tttccagctg
    14161 cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc tgcaagtacg
    14221 acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt tctatcaagt
    14281 aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca gttgattaat
    14341 ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc gcaaatgctg
    14401 agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga tttctttcaa
    14461 aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat catgtcgaat
    14521 attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt tgattattta
    14581 gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc tttgaatccg
    14641 gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga agtaacagca
    14701 gaagaagcag cagagagtct ttatgagttt gcgtcaaaca ttctaggata gcagcaggag
    14761 gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc aagacaatgt ggtcggttac
    14821 gtatttgctt ctccattcat cctaggattt ctattgataa cagtgatacc gatgatcttg
    14881 tcactttatt attcgttcac cgattatcgt ttgggaaatc cagtctcatg gatcggcttg
    14941 gataattata taaagatgct gagtgatgcc cgttttttga actccatccg agtcactttt
    15001 ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg gtctattgat tgcttatctt
    15061 ttaacgagga atatcaaagg aacgactttc tttcgttctt tgtattatgt gcctagtttg
    15121 atcggtggca gtatttctat cgctctagtt tggcgggagt tgttttctaa aaacgggtta
    15181 gtgaatattt tcctggaacg attaggcgca cctgcgcttt cctggtttag cgatcaaaaa
    15241 ctggtcatga tccctcttgt tttgatgtct gcttggcaat tcggttcggc aatgatcatc
    15301 tttgctgcag gtatcaaaga gatcccaggc tcgtattatg aagcggcaga gatcgatgga
    15361 gcgaacaaat ggcagacgtt ttacaagatc acgttacctc ttctttcgcc gatcattttg
    15421 tataacttgg ttatgcagac aatcgcggcg tttatgactt ttacgcaggc atttatcatt
    15481 acccaaggtg gtccgaatga tgccacgaac ttttatgctc tttatgtgta taaccaagct
    15541 ttttctttta attctatggg gtatgctagt gctatttcgt ggatcatgct gttgattatg
    15601 ggagctgtca cctttctgat tttcaaatca tcaaaaaaat gggtcgttgt gtgaatgaga
    15661 ggaggaagaa atgaataata cagtaacgaa atggtttcaa tacggtttta ttttgctatt
    15721 aggtctagtc atgatttatc cggtcttatg gatgatcgca ggttctttta aaacggatca
    15781 agagattatg agtggttcgc tgaatttgat tccagagact tttcaatggg aaaattacgc
    15841 aaaaggatgg gcggggtttg ctggaatttc attttttact ttctttagga attcgtttgt
    15901 catctctgca gtttcgacga tagggacagt tctgagctcg acttgtattg cttacgcatt
    15961 atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc gtcatgttag taacgatgat
    16021 gattcctgca caagtgattt tgattccgca gtttattatt tacaatcggc tgaatttggt
    16081 tgggacgtat gtgccgctga ttttaccaca tttttttgga caagcatttt tcatttacca
    16141 gatcatgcaa ttcatggtca gcatccccaa agagttagat gaatcagcga ttattgacgg
    16201 atgcagcaag tatagtgtct ttaccaaaat tatttttcct ttgctgaaac cttcgatcat
    16261 cacgacgatc atcatccagt tttattggaa atgggacgac ttcatgggac cattgattta
    16321 tttaaacaag ccgagatctt acacggtatc gattgccatc aaattatttg cagacgctgg
    16381 atctagtacg acttacgcga gtatgtttgc gatgtctacg ttgtcgctgc taccggtttt
    16441 tctgattttc ttattcttca ataaatatct tgtccagggg atcagtacaa gcggtctgaa
    16501 gggctgaata tgaaaaagcc aatagaagag atgtttcttt acaagctcaa ctactatgtc
    16561 tgtcggattt ttttaaccag tcttatcagt aatgctcttt tcgctttagt tctaggatgt
    16621 ttgttcactt taggattagt gctgtctgct cgtgttgtct atctgattta ttttttgatg
    16681 atgtatcctt cttattatct atctgtccat tacttggcag aaaaaaaggt atgttggagg
    16741 aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt ttcagctgtt tgtcattatt
    16801 tttgcagttg attggtattt attagtggga aagattggag cttggtatct gattcctttt
    16861 ctattgtgtc tgttgctttt tgtatcccat agttttttta gtacgctaat tgatatctgc
    16921 aaagaagata aagcagatag tcttgtttat ttgatcaaaa ggaatatctg gctgttgtca
    16981 aaaaacgtca aggggtttct gtggtattta tgccttttgt atggactgtt ctggtgtata
    17041 cagcatgatt ttttactcac tgcattactg tatccagggc tgccatttgt cacagtattt
    17101 tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa atatgtacaa gtcggacttg
    17161 gtgggagagc gcgttttttt tatgaggcaa ttgcctctac gtatcatcaa acttgtgaac
    17221 tagtcggttt ttgtgatgta tctcaaacaa ggatggatta tgcaaatcgg atacttgtga
    17281 acaagtatgg acagaaaaaa gtaaagacat gtatgccatc acaatttgag caactactgg
    17341 atgacgtacg gccggctgaa gtcattatta cgtcagtaga tcgaacgcat catgattata
    17401 tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga aaaacctctg actatcgatg
    17461 aggaaaaagc tcaagaaata ataacggtcc aaaaacagac gggaagaagc ctgcgagtca
    17521 cttttaatta tcgttatgca ccgtatcaca cgaagataaa ggaactgata gaaagtgatg
    17581 tgattggtga tgtgttttct atccacttcg aatggctgtt aaatacgcaa cacggggcag
    17641 attatttcag aagatggcat cgaaataagc acaatagcgg cgggcttctt gttcataaat
    17701 ctacccatca ttttgatcta gtgaattttt ggttgaacag ccgtccccaa aaggtatttg
    17761 cattcggtgg attaaacttt tatgggatgc ataatgcgga gaaacgcgga gtagaaatat
    17821 tttacagtcg atgctatgga agcgaactag ctaaagctga tccatttgct atagacctgt
    17881 cagccaatga ggaattgcga gaaatgtatt tagaagcaga aaaagacagt ggatatattc
    17941 gtgaccgcag tgtattttcc gatgatatct ctattgaaga tacactaggc gtaacggtca
    18001 aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc gtatatgccg tgggaaggat
    18061 tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat gaaagtgatc gaaaagtctt
    18121 acatcaatgc aggtggagat aaaaaggatg aaggagcagc cgttcttcag tccatccatg
    18181 tctatccgat gttcggtgag ccatatgagg taatcgtcga acaagaagcg ggcggacatg
    18241 gcggaggaga tccgcgttta ttagatgatc tatttggtga agaaaaagaa gaagatccgt
    18301 ggaatcgggc agcaactcac gtagatggta ttttgtcgat tttgacagga atcgcagcaa
    18361 atcattctat tgcttcagga aaagcagtag tgattgacga gcttgtcagc ttaaaagaat
    18421 aagaatagat ttacggaaat tacttcccgt agtttataaa atcttgcctt attgccgata
    18481 ggctgtcttt tgaacactat ttattcggaa aaaggggcta tgacatgctt tttgtcatgc
    18541 catcttttta tgtgggctgc ttttttagat aggagaaaat aaaaataact atttaaaccc
    18601 gaacaaaaag gaataatgca ttaaaaatca ttagatttta caaaaaaata agtaaaccac
    18661 gttgtcagtt tcagaaagta gtgttagact gttttcaata tttgaaacaa cattttttat
    18721 tcaaatatgg caaactgcct tcacgtgcaa aacagttgca agaaaatgaa tttacactat
    18781 tattagtttt acatacctac ttatatatcg tcataatatg gatgacagtt tctagccagt
    18841 accgtaaatg ctggactata agtaaaagat tggctatttc acccttcttt tcttatgata
    18901 aagtggaggg tgtttttctt taacagaaac atagtaggtt tttgcacaaa agcggttatt
    18961 tcggtattaa taaatgataa aggagcaaat aaagtgaaag aattagttga acgtattcaa
    19021 aaagacggac gtatattagg cgagggcgta ttaaaagtcg acagcttcgt cactcaccaa
    19081 gtagaccctg tattgatgga acaaatggga aaacggtttg cagaagtatt taaagaacaa
    19141 ggaatcacga aagttgtgac cattgaagct tctgggatcg caccagcttt atttgctgca
    19201 agagaattag atgttccaat gatcttcgct agaaaagcaa aaagtttgac gatggatgaa
    19261 gaattattga cagcttctgt ttattctttt acgaaacaag tgacaagtac gatttctatt
    19321 tctcgtaaat ttttatcttc agaagacaaa gtattgatta tcgacgattt cttggcaaac
    19381 ggacaagcgg caaaaggatt gatcgaatta tgccagcaag ctggagcgca agtagaagga
    19441 atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc agttacttga agaaatggga
    19501 ttgagagtcg tttctcttgc gcgtattgct tccttgacga atggacaagt tgagtttctt
    19561 gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg aaaagctgcg gttttagggc
    19621 tgcagcatct gctagcgatg tatgcaggag cagtagcggt gcctcttttg atcggtacag
    19681 gactaggctt taatcaagaa cagatgactt atttgatttc aattgatatc tttatgtgtg
    19741 gattagcgac attattgcaa ttgacagtta accgattttt cggaattggc ttgcctgtcg
    19801 ttttaggatg tgcgatccaa gcagtagctc cactgatctt gatcggaacc aatgaaggag
    19861 taggagctat ctacggatct attatcgctt ctgggatctt tgttgtgtta gtttctggtg
    19921 tcttttctaa aattaaaaaa ctattccctc ctatagtaac aggaacggtt atcaccgtta
    19981 ttggtttgac tttgattcct gtagcaatcg agaaaatggg tggaggtaat gcaacagcag
    20041 ctgattttgg tgacaaaaca aatctgctac ttgcatttgt gacgattctc ctaattgtag
    20101 gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc ggtattgatt ggtttagtcg
    20161 gaggaagcat cttagctgct ttcatgggaa tgattgatct gagcgcgatt ggagatgcgc
    20221 cgttgttcca tgttccacaa cccttttatt ttggtaaacc aactttcgac gtatggtcga
    20281 ttttactaat gatcatcatt tccatggtca gcatggtaga atcaacaggt gtatattttg
    20341 cacttggtga cattactggg aaaaatgttg gagaagaaga attgaaaaag ggatatcgtg
    20401 cagaaggact tgccgttatc ttaggcggta ttttcaatac attcccttat actggctttt
    20461 ctcaaaatgt cggattggtt caactttcgg gaatcaaaac aagaaaaccc atctatttct
    20521 ctgcattctt cttgattgtt ttaggattat tccctaaaat cggtgcgtta gcacaaatca
    20581 tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt cgggatggtg gcagtccaag
    20641 gaatccgtat gctgttggaa gttgatttca gcaatgataa aaatctattg attgttgctg
    20701 tttctgttgg atttgggctg ggtttcaatg ttacgccgac attattcagt caaatgccgc
    20761 aaacattgca aatgttcact ggaaatggca tcgtaatgag tagcattacg gctatcatat
    20821 tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat attggaagaa gccttcaaga
    20881 agtaatagag caaaagaaag gttgtgaaat cagcgttttg acctgattgt tagagaaaca
    20941 aacggaaaaa tagttcctcg tattttgccg tttgtttgag ctaataccga gggtcagctg
    21001 attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa
    21061 caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt tccttgtgac cgaggagttg
    21121 gcaggagaac accgtttatc cataaaaagg aatgtgacct aacttttgtc acattccttt
    21181 tttttatctt taataaacac gaacaataaa atgtttttct ttgttaacat tcgaaatttc
    21241 tttgacatca aaaaaagtga ttgttaaaat atgaatgtag aaaaactggg aggaaaaaca
    21301 atgacagcaa aaatctctgt gattatgggg agtacttcag attgggaaac aatgaaagaa
    21361 gcatgtcaaa tattggatga attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat
    21421 cgaaccccag atttgatgtt tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc
    21481 attattgcag gagcaggggg agccgcgcat ctgccgggta tggtcgcatc aaaaacaact
    21541 ttgccggtta tcggtgtacc ggtccaatca cgaacattaa atggtttgga ttcgcttctt
    21601 tcgattgtcc aaatgcctgg aggagtacct gtagctacta cagcaatcgg aaaagcggga
    21661 gcaattaatg ctggactact tgcgatacag atgctttcca tgtatgattt agaattggag
    21721 gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa
    21781 cttggttaaa cctttgttgc ctggttcaac gatcgggatc gtcggtggcg gtcaattagg
    21841 tcgtatgatg gcgattagtg caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt
    21901 gagcgattgt ccagccgcac aagttgcaga ttggcacatt gaaagtgtct atgatgacac
    21961 ctttgcgctg aaagagcttg ccagaagaac agatgtcatt acctatgaat tcgaaaacgt
    22021 cagtgtagag gcgttaaatg cgattttacc catgtcattt attcctcaag ggacagactt
    22081 gcttgcgatt acgcaagacc gcctgttaga aaaatctttt ttggaaacga ataacatcgt
    22141 gattgctcct tatgcaacga tcgtcagtcc gacggatatc caagatgcga ttgacgggat
    22201 cggctatcct tgtgttttga aaaccactcg aggcggctat gatggcaagg ggcagtatgt
    22261 tttgaaaagt cgggcggatc tagctcctgc catggacctt ttaagagaag gaacctgtgt
    22321 tttagaagca tggatcccgt ttgaaaaaga gatttcgatc atggtggcag gaaatggtca
    22381 aggagatttc acgacttttc cggtcgtgga aaacatccac cacaacaata ttcttcatga
    22441 aacgattgct ccagccgcga ttgatcagga tgtgatcgag gaggctgagc gtattgcccg
    22501 tgtgattgca gaagcagtga gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa
    22561 aacaggcggg ctatatgtga atgaattagc tcctcgtcct cacaattctg gacattattc
    22621 tattgaagct tgttctatga gtcagttcga tgcccatatt cgaggtattt gtggttggcc
    22681 attaggagaa aaagtacgtt tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca
    22741 actgctggaa agctatcgct tgattgagaa taagccagac tggcaattcc atttctatgg
    22801 aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc actatcttga cagaagacat
    22861 ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg gattagagga gaaaaaacat
    22921 gttagaacgt tatacacgcc ctgaaatggg gaatatctgg acggaagaga acaaataccg
    22981 tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc
    23041 gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt gatatcgatc ggatcttgga
    23101 aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt
    23161 gggagaagaa agaaaatggg tccattatgg tctaacaagt acggatgtcg tagatactgc
    23221 ttatggctat ctcatcaagc aagcaaatga tattttacgg gaagacttga aacgttttac
    23281 ggaaatcatt ggcgaaaaag caaaagaaca caaatataca gtgatgatgg gtcggacaca
    23341 cggtgtccat gctgaaccaa ctacttttgg attgaaactt gccacatggt attcagaaat
    23401 gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc
    23461 aggagcagtg ggaacgttcg caaatatccc gccatttatt gaagagtatg tatgtaaaca
    23521 attggggatc cgtccacaag aaatctctac tcaagttctc cctcgggatc tacatgctga
    23581 atatttctca gcgatggcgc ttattgctac aagtattgaa cgttttgcaa cagagatacg
    23641 cggattgcaa aaatccgaaa cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg
    23701 ttcttctgct atgccgcaca aaaggaatcc gatcggttca gaaaatatgg ctggtctagc
    23761 cagagtggtg cgcggacaca tggtcactgc ctatgaaaat gtcacattat ggcatgaaag
    23821 agatatttct cattcttcag cagaacgaat catcttgcct gataccacga ttttattgaa
    23881 ttacatgtta aatcgttttg gcaatatcgt taaaaatcta acggtctttc ctgaaaacat
    23941 gaagcggaat atgtctgcaa catttggttt gatttatagc cagcgcgtta tgctgaaatt
    24001 gatcgataag ggaatgacaa gagaagaagc ttatgatctt gttcagccaa aaactgcgta
    24061 cgcttgggac aaccaagtgc agttccgccc gctgttagaa gaagatccaa ccatcacatc
    24121 ggtattatct aaagaagaaa tagacgatgc ctttgattat catcatcatt tgaaaaacgt
    24181 tgacgtgata ttcaaacgag taggtttgga ataaagaaga gtgagttgca aaattcaatt
    24241 aaaatagaat agcaaaaata atcgagtaga ggggaaagcc aactgacctt ccgcccctct
    24301 cacaccaccg tacgtacgga cccgtatacg gcggttcaat aagttgagtg tacgaactgg
    24361 tacttcaagg agatgtcctc atatccttgg cgggccagtt ctttgtctgt taatgatcga
    24421 tgaagaatat gacttttgga gactgaccaa ataccttttc ttgtatttga ccattcatac
    24481 gctttcgctt tatcgatccc taaacgaatc agattttttc ttttggttct tggattcttc
    24541 cattgtttcc aaatatattg tctgattctt cgcttcaacc aaccgtttaa ctccctcatg
    24601 aattctttca tctcaccaat tccatagtag ttgatccaac cagtcattag ttgcttgatt
    24661 tcttttaaaa taagttggat acttcttcca cggtttcttt tagtgagttg tttgagcttg
    24721 cttttcacac gctgttttgc ttttgcgtga gggcgaattt taataccttt ctttgttggc
    24781 agaatgcaga atccaagaaa ctttcgtttc aatggacttc ctaccgcact cttttctcga
    24841 tttaccgtca actttagttg ttcttcgaga aattgaatgg atttcttcat cacacgctgt
    24901 cctgctcgtt tgcttctaac atagatattg cagtcatcgg cgtatcgaac aaatttatgt
    24961 cctagatttt ctagcacttg atcaaattcg ttcaggtaga tgttacttaa caggggagag
    25021 agattgcctc cttggggcgt tccttcttca gttgttgtga tgagtccatt ttccattaca
    25081 ccactcatta agtattgacg gatcagtttt aggacttgtt tgtcctttac tttccgctca
    25141 atgaaataca tcagcttatc gtgatttacg gtgtcaaagt aggctttcat atcaatgtcc
    25201 accacatatc ggtaaccttg ttcataataa gattttgctt tcttcactgc gtcatgtgcg
    25261 ctgcgatttg ggcgaaaccc ataactatta tctgagaatt ctggttcaaa tagcggttgt
    25321 aacacttgat tgattgcctg ttgaagcata cgatcaataa ccgtgggaat tcctagtttt
    25381 cttacaccgc cgtttggttt cggtatttct actcgcaaaa caggctgagg tttatagttc
    25441 ccttggcgta aattggatag taattcctcg cggtgctctt tcaagtaagg aagcagttga
    25501 tcgacggtca tcccatctgc accagccgcc cctttatttc tgactacttg aagatacgct
    25561 tgattgagat tattccgatc tagaattctc ccaaacagat tttctacacc atcttgtttc
    25621 ctctgttcac cgcaagaagt gctgcgcact tcatcatacc cttcgtgttc cacactagct
    25681 ctttgatgat agccaagttt tcttgttttc tgcggtcgtc gcatcttctc acacctccta
    25741 aattattcaa gattcttatt gttcagtcct tcgtaacggt cagtgtcgtt actactatga
    25801 cctcggctga cttctcatga ttcaccatca tgtcgccatg atcgttgtgc tggtttgttt
    25861 tcattcaacg cccacaacac cctcatgaga tctcccccgg taagagtgaa aactttcctc
    25921 ccatgtaact gctagattta ctgtataggt ttcgggcagt attggacttt gtcttgtgat
    25981 gaagacttat ccgacctaat tcagccttgt atctagtttc tgttcgtcag ttcaggagtt
    26041 tgcgtccgac ttccttcagc cacaacctca cgattgccgc cttgtctttc gctaacagtt
    26101 cctactgcca agcctgtagt ggactttcac caccaagttt tcacccatgc agggcgcaca
    26161 ccaaaaaaga agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat
    26221 caataatagg aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa
    26281 aaaatagagg aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag
    26341 atgttgcagc aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg
    26401 gatacgataa tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc
    26461 tttgtgttct gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc
    26521 tcggcggatt tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt
    26581 gatcgacagc aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa
    26641 aagctgacct tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca
    26701 aatttttagt taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt
    26761 ataagatttt cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac
    26821 atatttttgt tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa
    26881 aaacgatgta tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa
    26941 cttttgtatg aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg
    27001 atagaatatt tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga
    27061 aaaggagaat tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga
    27121 ataaaaaatc attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa
    27181 attatccctc tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta
    27241 ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac
    27301 cagttggatg atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg
    27361 gaagaaatca gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta
    27421 tttgctaagg tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga
    27481 caaattttgc ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg
    27541 aatgaacata tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat
    27601 caagaagtat ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt
    27661 tcgtaaaagt atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa
    27721 aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact
    27781 ttgagataca acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg
    27841 acaagctgct tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg
    27901 aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg
    27961 ttatgggcag ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc
    28021 tctttagaag gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg
    28081 agatgcggtg cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa
    28141 gaagggaaac cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt
    28201 ttgccaggtg tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg
    28261 aaagtcaacc ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt
    28321 ccagttgctc atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa
    28381 aaaaataatc agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt
    28441 gcagggatta cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca
    28501 gtagaagact tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac
    28561 ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag
    28621 aaaaaaagaa tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa
    28681 gatatcttag gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc
    28741 gaacattgtt cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct
    28801 catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca
    28861 gttgtcttca aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct
    28921 gcgacaggag ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg
    28981 ttgctagatt cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa
    29041 gaagtagttg caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc
    29101 gaaattgcat ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt
    29161 ttgattgaac acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg
    29221 tatgtaggtg caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa
    29281 tttaatcaag aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa
    29341 aagctgctgc ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt
    29401 atacaggata tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct
    29461 ggctcaggac ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct
    29521 tatgaaatga tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag
    29581 gaagaaaaaa tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa
    29641 gtaacagacg acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca
    29701 gtcgatgcat tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga
    29761 atcgtagaat ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta
    29821 ttgcttgatc tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat
    29881 gattcacaag tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg
    29941 gttagaggga cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat
    30001 ttggatccaa aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct
    30061 agcggtggta agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca
    30121 gaagtttttt gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta
    30181 ggaactcctg ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc
    30241 tatccaacac caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact
    30301 caggaattca aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt
    30361 gatggcagtg aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt
    30421 gatctatcca ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg
    30481 attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct
    30541 ttcaaacatc agctagggat atctgtacag tttgaattgt cctctgctca attatttgca
    30601 gaaactcaat caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa
    30661 atgatgggag atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt
    30721 tcagcaactg acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat
    30781 gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt
    30841 ggggtcatcc agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag
    30901 gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt
    30961 tggggctgct ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag
    31021 ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc
    31081 cttttctatt caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca
    31141 atgctcgttc ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg
    31201 atacggagat attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat
    31261 taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg
    31321 caatgattgc cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa
    31381 acggagcata cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta
    31441 taagagatgt ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt
    31501 gctttacgaa ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac
    31561 cagattcaaa catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt
    31621 tagcgaaaga atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt
    31681 cagccgcaag cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa
    31741 atcaatacat tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc
    31801 gaatgaaatt atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg
    31861 attcaattgt tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg
    31921 caaaagaagt ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga
    31981 ttgatattca aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag
    32041 aatgtatcgg tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg
    32101 gtctggaaaa agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc
    32161 cgacgccgtt atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt
    32221 tcttttaaaa gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa
    32281 gctggcgtag atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa
    32341 aaaacggaac gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta
    32401 tcggtctttc aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa
    32461 ttagcagtgg caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg
    32521 tgtgtcaatg atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca
    32581 acggggaaaa atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt
    32641 gttcaaggaa atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca
    32701 ggtaatgatt atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg
    32761 acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat
    32821 tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca
    32881 tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc
    32941 tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc
    33001 actggtggag ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa
    33061 atcaaacttg gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa
    33121 attcctgaga tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc
    33181 caagaatcga aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat
    33241 gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga
    33301 tgagaatcgc tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc
    33361 tatcgaaaaa aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat
    33421 atgttttgaa acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat
    33481 ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg
    33541 atttgatcgt attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg
    33601 ataaaagaat catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca
    33661 tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc
    33721 aaggtgtaga tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca
    33781 cgttcgattc tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga
    33841 ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc
    33901 agataaaact gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat
    33961 ttcaacagga ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga
    34021 agaagtgaca gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat
    34081 ccatggtggt cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga
    34141 aaatatccaa ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat
    34201 gaaaccagat gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat
    34261 gctccgtagt gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta
    34321 tgaagaagta ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa
    34381 actagcggca aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt
    34441 aacggattgg gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca
    34501 aacgcttcgt tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct
    34561 ggaaaaatat tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa
    34621 tatccgagat gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt
    34681 cgccttgaaa cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg
    34741 ggaagcagcg tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg
    34801 agaagtagat ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc
    34861 cccttcttat acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact
    34921 gacagtagac tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg
    34981 tggacttctt gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt
    35041 gacaaaacgt aagccgactc cagcagaagc aaaagcatta gcgtttgctt ggagagctgt
    35101 caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg
    35161 agccgggcag atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa
    35221 aatggaaggt gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata
    35281 tgcagccaaa catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga
    35341 ttctattgat atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt
    35401 tagacattag agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga
    35461 acatgcaata agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc
    35521 aggaaatccg gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca
    35581 ttcagcgtta atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga
    35641 agttcctttg ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg
    35701 tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga
    35761 tacttatcaa atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc
    35821 gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa
    35881 aggtgtagtc gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa
    35941 ccagcatcga tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga
    36001 agaattctcc cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca
    36061 agatcataag cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta
    36121 ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa
    36181 accagcggct ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg
    36241 tttgattgct acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc
    36301 tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt
    36361 attagagaat cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt
    36421 ggttgcagct gccggttacc ccgaagaata tgagacagga atggttctac cagattttct
    36481 ggaagaagag ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg
    36541 agcaggagga cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa
    36601 aaaaatttac accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat
    36661 tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata
    36721 aaaaatacaa caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg
    36781 tggtacactt ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag
    36841 gtacttctga tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg
    36901 aagatccagt aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg
    36961 cagatattta tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat
    37021 caatcaaacg agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt
    37081 ttgatttttc taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa
    37141 tggattatgt ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag
    37201 tagctgaagc gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca
    37261 atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta
    37321 atcagctgca atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata
    37381 tgacagatga agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg
    37441 atatgacgat ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg
    37501 ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa
    37561 cgacaccaac tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga
    37621 tcgccggtac gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata
    37681 tgtctcggga agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat
    37741 tgtcataagc aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt
    37801 tcagcttcga gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat
    37861 ttcgtttatt agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa
    37921 atcccttgtt ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca
    37981 aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt
    38041 ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg
    38101 aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg
    38161 gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt
    38221 gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa
    38281 agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct
    38341 tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa
    38401 cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta
    38461 gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa
    38521 tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg
    38581 gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt
    38641 ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca
    38701 tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca
    38761 actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc
    38821 ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact
    38881 tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca
    38941 gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga
    39001 ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct
    39061 aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc
    39121 agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca
    39181 ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac
    39241 ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt
    39301 gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa
    39361 ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata
    39421 aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt
    39481 gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta
    39541 ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc
    39601 gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc
    39661 gttgtctata actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa
    39721 tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa
    39781 gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag
    39841 catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt
    39901 ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc
    39961 atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc
    40021 caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg
    40081 tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat
    40141 tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa
    40201 gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg
    40261 gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac
    40321 tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct
    40381 tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac
    40441 agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt
    40501 attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa
    40561 cgtactaatt tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag
    40621 ttcttctagg atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc
    40681 ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat
    40741 atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag
    40801 caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat
    40861 gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta
    40921 tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg
    40981 tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt
    41041 tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc
    41101 catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag
    41161 ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat
    41221 catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga
    41281 caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg
    41341 ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt
    41401 tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat
    41461 tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga
    41521 cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg
    41581 ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga
    41641 tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca
    41701 tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt
    41761 cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg
    41821 ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa
    41881 gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact
    41941 atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga
    42001 aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa
    42061 aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg
    42121 ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag
    42181 cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg
    42241 aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc
    42301 ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa
    42361 tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc
    42421 tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt
    42481 gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga
    42541 taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga
    42601 aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact
    42661 aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt
    42721 cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac
    42781 ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc
    42841 tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg
    42901 gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg
    42961 cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt
    43021 agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa
    43081 tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt
    43141 atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc
    43201 cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg
    43261 ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga
    43321 atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc
    43381 ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat
    43441 ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa
    43501 gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt
    43561 tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg
    43621 gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg
    43681 aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca
    43741 ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa
    43801 gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc
    43861 attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg
    43921 gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc
    43981 ggaatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa
    44041 gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata
    44101 aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg
    44161 ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata
    44221 tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt
    44281 gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg
    44341 gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta
    44401 gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca
    44461 aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta
    44521 tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata
    44581 gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa
    44641 gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat
    44701 acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc
    44761 ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac
    44821 ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta
    44881 cagcatggtg atcattttag cgggactact tattgcgttt caattgatga cggatggtat
    44941 tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata tattagtact
    45001 agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt
    45061 gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg
    45121 gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg
    45181 ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg
    45241 tggactgaca caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca
    45301 aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac
    45361 attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa
    45421 tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt
    45481 atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc
    45541 gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac
    45601 agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg
    45661 gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt
    45721 agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat
    45781 ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg
    45841 aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc
    45901 gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc
    45961 cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc
    46021 agaaaaaaga ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc
    46081 attgtttcgt ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct
    46141 ctttttgctc ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc
    46201 cttgattaaa ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa
    46261 tgaaagtatt tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc
    46321 gttctgaatg tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc
    46381 ctgccaacat gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt
    46441 atttctatat catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga
    46501 aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta
    46561 tagagaagtt ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg
    46621 gacatgctaa ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt
    46681 ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg
    46741 cagatgcaac aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag
    46801 gctttgggac tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca
    46861 agccgatcat cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt
    46921 ttggtgcgac aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg
    46981 aaacaaaagt cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc
    47041 aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt
    47101 tgaaagatac actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag
    47161 gaagagatct ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta
    47221 atggagacac aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc
    47281 attaagtaat aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat
    47341 tattatcgca gatacaagct tttgaacatc attattcaga ataagggact gtgataagat
    47401 ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt
    47461 aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa
    47521 aaaacaagga ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg
    47581 aaggatcaat cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt
    47641 gaatcgttcc accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag
    47701 caaacgtggc agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag
    47761 aatcgattgg cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa
    47821 aataagagaa gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat
    47881 gccagatgag cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc
    47941 ggaagaaaaa gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta
    48001 tttggaaagt aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg
    48061 tcagcaagca atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt
    48121 agagtcacct tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt
    48181 tgggataccg atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa
    48241 aaataaattg aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat
    48301 gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat
    48361 tgaagacgat gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg
    48421 aacgattgat ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac
    48481 aacgaaaatc ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca
    48541 agaaatgatg gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa
    48601 tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact
    48661 gataagcgct tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta
    48721 ctatgcctgg attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt
    48781 agatatgggt cttggaattg caccaagtct actttttggg caagatataa atggaatcag
    48841 aatcaattac agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat
    48901 tctagaaaag tggttaacag aataatttca caaagtaaat agattaaaga atataacagc
    48961 ttggatttaa atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac
    49021 taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca
    49081 acaaaactgt ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga
    49141 aaatagaaaa aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa
    49201 tgctgttaat atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct
    49261 tgtatttctc ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt
    49321 ttattcactg tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt
    49381 gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg
    49441 cagatttccc agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag
    49501 tacctgattt atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg
    49561 tattagacca acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa
    49621 ctgctggaca agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc
    49681 ttcttccagg ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag
    49741 cttttttatg gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc
    49801 taccaccaca aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt
    49861 tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac
    49921 aaggtgattt ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt
    49981 atatccaaaa caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa
    50041 cacttgcaca aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc
    50101 cgcttgcagt atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac
    50161 cagtcctaat cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc
    50221 caacccgcta ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc
    50281 gcttccgtaa atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca
    50341 tcgaagcaac taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac
    50401 aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac
    50461 aaattgcaat ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca
    50521 agcaatcaca gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg
    50581 tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt
    50641 cggagaagac cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt
    50701 cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt
    50761 cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac
    50821 tagaaatcta cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt
    50881 gcattcggat aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc
    50941 ttccaaccct tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt
    51001 tgttttccta gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc
    51061 ttacgaagtt cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat
    51121 cacatacgga gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa
    51181 tatatctgtt gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat
    51241 tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg
    51301 cgttgccgcg caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc
    51361 aatcggacga gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg
    51421 gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa
    51481 gaagggacga aacaaagtca tggcttatca atttaaattg ccggatatcg gtgaaggaat
    51541 cgcagaaggt gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga
    51601 tacattacta gaagttcaaa atgacaaatc agtagaagaa atcccgtcac ctgttacagg
    51661 aacggttaaa aatgtgattg ttccagaagg taccgtagca aatgttgggg atgtattagt
    51721 agaaatcgat gcaccaggac acgaagacaa tgaaggggac agtggtgtag ccgcagaatc
    51781 ccaaactcca gctaaaccgg ctgctgaacc cactgttgat acagaatcag ctggatcgtc
    51841 aagcgaagga gtcttccaat ttaagttacc ggatatcggt gaaggaatcg cagaaggtga
    51901 aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga
    51961 agttcaaaat gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa
    52021 cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc
    52081 tcctggacac aatagtgcac cgtcaactag tgcgccatca gctgaagcac caaaagaaaa
    52141 agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt
    52201 ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac
    52261 tgcaactgga aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg
    52321 tccaagcagt gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc
    52381 tcctgcagca gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga
    52441 cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa
    52501 cagtaagcat acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg
    52561 ggataatcgg aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt
    52621 gccatatgtc gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc
    52681 tatcgacgat gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac
    52741 tgatacggat cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt
    52801 tgctattgca gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc
    52861 agaagatatg cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg
    52921 gtttacacca gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca
    52981 acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt
    53041 aagtttcgat caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa
    53101 acgtctttta gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg
    53161 gagattttgc tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag
    53221 ctgccatccg cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg
    53281 gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc
    53341 attatcaaga gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag
    53401 attttgcaaa aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg
    53461 ttggtttcct attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag
    53521 atgatcacac actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg
    53581 caatcatcgc aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg
    53641 tgttagattc tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg
    53701 gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa
    53761 cgattcttga aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag
    53821 tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag
    53881 cagtcgataa cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg
    53941 ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct
    54001 tagaacaagc gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac
    54061 gtacgaacgt acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac
    54121 acaaagccag ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg
    54181 tagattataa agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta
    54241 tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt
    54301 cagggaacgg tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga
    54361 cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg
    54421 tctctgaatt agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga
    54481 ttcatcctca tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt
    54541 taccaatcca tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt
    54601 tttgtttttt agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt
    54661 ggtttattac cacttcctat gctacactga agaggaatat gaaataggag agttgaagat
    54721 gcaagaatat cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat
    54781 gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt
    54841 gaagacttat cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg
    54901 acgggaattt gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa
    54961 aaatccaaac aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat
    55021 atcaagtcct ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca
    55081 atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat
    55141 tttctaatcg aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa
    55201 gggtgtaatc aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc
    55261 accttgaatt ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc
    55321 ggcgttggaa agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga
    55381 aaaggaatag gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta
    55441 tcggaaggct tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga
    55501 gaaattgcgg aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg
    55561 gcaatcttga aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca
    55621 gcgggaaatg tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct
    55681 ttgacatttg aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc
    55741 gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa
    55801 aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa
    55861 aaagtctgct ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa
    55921 aggagttagt tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg
    55981 ttgaccacgg aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg
    56041 cacatacgca attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta
    56101 tcacgatttt agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg
    56161 atactccagg acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg
    56221 gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga
    56281 aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt
    56341 ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag
    56401 atgatgatca gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct
    56461 tgtcagatga tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg
    56521 aaaaaatccc tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac
    56581 ttgactacaa cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca
    56641 aagtaggaga ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa
    56701 cgaaattatt tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag
    56761 acttgattgc tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg
    56821 atcatcaaga tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct
    56881 tagttaataa ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg
    56941 aagaacgttt gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact
    57001 ctccagatgc atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa
    57061 acatgcgccg tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa
    57121 tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata
    57181 tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata
    57241 caggaaacgg acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt
    57301 caactgaatt cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat
    57361 atctgccaat gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg
    57421 atacaggaaa agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg
    57481 tagaaccagg tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg
    57541 atttgactgt caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag
    57601 accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct
    57661 tgaatgatga tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt
    57721 tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa
    57781 gagagaaacc agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg
    57841 tggagaagtc atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa
    57901 agacttttaa aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg
    57961 aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg
    58021 gaagatgaac gacttattaa aacaatactt tggctatgat gagtttagac ctggtcaaaa
    58081 agaaattatc cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag
    58141 tgggaaatca atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt
    58201 gtccccttta atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc
    58261 cgccactttt atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat
    58321 cgatagacag cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt
    58381 tatccaagca atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat
    58441 ctctcagtgg gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca
    58501 actgccaaat cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc
    58561 tgacataaaa agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg
    58621 agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata
    58681 tttaaagatc aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga
    58741 ccgtctctat catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct
    58801 aaatgagaat gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat
    58861 tgtagcgaca aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca
    58921 ttatcaaatt cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga
    58981 tggtctttcc agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt
    59041 ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa
    59101 agcgatgaca gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt
    59161 tggcgagaca agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt
    59221 agaagtaacg acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta
    59281 tggtaaacaa atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt
    59341 aggttttgga catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat
    59401 gcagttaatt gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc
    59461 tgttttgaaa gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg
    59521 aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt
    59581 gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt
    59641 ttcagattcc acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct
    59701 tcagataaaa ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg
    59761 gataaaaaat gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga
    59821 ataaaaacaa ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa
    59881 ttaaggaatt agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc
    59941 caagtattaa atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt
    60001 ctctgtatct cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt
    60061 tcccggcaag tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg
    60121 atgttaagtg agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt
    60181 tatgataaaa aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc
    60241 atctattaga ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca
    60301 tggtatatag ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag
    60361 tcattaacca gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa
    60421 tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga
    60481 ttttgctgct gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc
    60541 aaattgcagg attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc
    60601 tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga
    60661 aacgtccact tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg
    60721 gtaatgcggc agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt
    60781 atgtgtatac tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc
    60841 tgatcaatat cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat
    60901 ttgctattta ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg
    60961 gctactatgc catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga
    61021 aaaaaggatt tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac
    61081 tgggagtgat tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg
    61141 tccttgtagg tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg
    61201 caatgttttt gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga
    61261 cagggatcac gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt
    61321 gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat
    61381 aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc
    61441 gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca
    61501 gaagaagatg aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa
    61561 ggaaaaaaac cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa
    61621 tgcggagctg acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg
    61681 caacggtgcg aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt
    61741 tttggagata aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca
    61801 ggcacagatg gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt
    61861 tatccgatta tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac
    61921 gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt
    61981 ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag
    62041 attctcgggg atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag
    62101 cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt
    62161 caaaaaatct gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt
    62221 acagtagaat ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc
    62281 caagtggaac atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg
    62341 ttgattgctc aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt
    62401 aaattaaatg gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt
    62461 ttaccagaca cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg
    62521 gatgtcggaa atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta
    62581 aaagtatgta cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg
    62641 aaagaattta gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca
    62701 catcctgaat tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta
    62761 tttgaatttc ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt
    62821 actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt
    62881 atgcctaaag atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg
    62941 aagggtgccg atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat
    63001 acgacattta gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc
    63061 aagcagattg cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg
    63121 gggggagcaa catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg
    63181 agaaaaattc gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat
    63241 gccgttgggt attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca
    63301 gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa
    63361 aaaagtatcc aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact
    63421 ggcgatatca acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca
    63481 aaggaactag aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt
    63541 aaaccgcagg cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc
    63601 catctgcata ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa
    63661 gcaggagtag acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct
    63721 agcatgagca gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt
    63781 gaaaatgcac agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt
    63841 gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag
    63901 tattcgaatc tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc
    63961 aaacaaatgt atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca
    64021 tctaaagtag ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat
    64081 atttatgaaa aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga
    64141 gaactagggc aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc
    64201 agacctgcat tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta
    64261 aaaaaagaat tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat
    64321 ttgatgtatc cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt
    64381 acattactgg acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta
    64441 caaatcgaac gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc
    64501 gaagggaatc gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa
    64561 gataattcga tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa
    64621 caaattggag caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac
    64681 gttaaaaaag gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa
    64741 gcacgttttg acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct
    64801 ggtgaccttt tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa
    64861 gactgggttt gttttggctg attctgctat tagttattgt ggcattctac atggaaccag
    64921 tcttgtttcc gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa
    64981 tgacaacgtg gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac
    65041 cgatcgatca gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg
    65101 gttttgaaac aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc
    65161 aagacagcag agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt
    65221 tttatttagg gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca
    65281 cttttgagta ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc
    65341 gtccgctaat tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa
    65401 acggcctatt ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc
    65461 aagtgtttgg tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa
    65521 tcaatcgcgt taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg
    65581 aacttcccat atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg
    65641 attttttgac aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat
    65701 cgctaggcag tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt
    65761 taatcagtaa acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg
    65821 ctactttttc gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc
    65881 cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag
    65941 aagaaaacgg agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag
    66001 gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt
    66061 gaacataaac agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg
    66121 tctaagaaag aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt
    66181 gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca
    66241 gctttccgat atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt
    66301 gccgaagaag tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa
    66361 aaacactttg gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac
    66421 aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa
    66481 gttcggcttg ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga
    66541 agagacattg gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga
    66601 acgttcttac cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt
    66661 attaaaagac gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca
    66721 tctttaaaaa tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt
    66781 tttgatcatt gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg
    66841 agtagatgcg gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg
    66901 cgaacaccag accaactaca gataaagtaa aagaatccat attcaatatg attggaccat
    66961 attttgatgg agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag
    67021 cagtatctag aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa
    67081 tcatcaaaga aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg
    67141 atgcggacaa agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc
    67201 ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg
    67261 gacttttttc taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag
    67321 aaactgtggc tggattccat caaataagaa aacagattta tggcataacg gccgtaacta
    67381 tctatagaaa agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt
    67441 tacaaaaggt cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat
    67501 tggtgttttt atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt
    67561 gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt
    67621 gacggtagaa acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat
    67681 caaagacttt gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt
    67741 ggaaactgtc ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa
    67801 agaagtactg cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc
    67861 tttagctgga aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta
    67921 tcatatttgg aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat
    67981 caccgggagc cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag
    68041 gttctgggtc tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt
    68101 taaaagctaa aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg
    68161 cgacaaatga agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag
    68221 caatcgaaca agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat
    68281 atgtcatgag tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag
    68341 taacaaaagt agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa
    68401 gccaaaaagt tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag
    68461 cttctggtga tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta
    68521 ctgaccatac cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg
    68581 ggccatcagc gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact
    68641 tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc
    68701 gaatcggagg aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct
    68761 ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca
    68821 actacgaaga agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag
    68881 taaaaacagt acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta
    68941 aataggggtt gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga
    69001 taaaaataaa aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca
    69061 gagctcactg tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt
    69121 ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt
    69181 gatcatttgg atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc
    69241 aaatttgcta ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt
    69301 tagttccagt ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc
    69361 aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc
    69421 ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga
    69481 tagcgatcga atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc
    69541 tgtaggtgaa gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc
    69601 tacagcagat tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca
    69661 gcagattcct gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa
    69721 cggatccttc cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt
    69781 tgaaaaacta aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag
    69841 caataaatgg acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga
    69901 ggaggtagta tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac
    69961 caatgctgaa tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg
    70021 tcagcggcaa taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata
    70081 cagcagataa agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt
    70141 ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag
    70201 ttctgatcat gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg
    70261 gaataacaag ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga
    70321 ttgtgaagta tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat
    70381 aatgggctaa tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac
    70441 aggatgattc gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac
    70501 ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa
    70561 taaagaaaaa gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat
    70621 ggctcaggca gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac
    70681 actcgttcga gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt
    70741 ccactgcata gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc
    70801 agagatgaat gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat
    70861 ctatgtcaca cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa
    70921 agggatcaat tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca
    70981 attaggtgta tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa
    71041 tgaattaaca ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt
    71101 ttccggcaat tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc
    71161 gtttgcttct tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt
    71221 acttgtgtat tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat
    71281 ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa
    71341 atggtaattt tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt
    71401 cttatcaagt aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa
    71461 tagaattgac tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt
    71521 ttgaccaaga agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga
    71581 ttgaacagat caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt
    71641 ttatctcctt tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat
    71701 tatggggcta taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt
    71761 tgctgcatct gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt
    71821 atttgtttcg acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa
    71881 ctatctgtta tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt
    71941 ttttattgcg attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg
    72001 cagttgtctt ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac
    72061 agcttagttt tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga
    72121 aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt
    72181 tttatgagtg gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt
    72241 ttctttttct tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt
    72301 tacttttgct gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag
    72361 ggtcgtctct cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat
    72421 tactgatttg tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc
    72481 ttttttcttt tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta
    72541 tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt
    72601 tgatcgatac tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac
    72661 gtcctcctgc tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg
    72721 ataaactcat cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc
    72781 agcattttac tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac
    72841 aaaggcattt atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg
    72901 acaggatcag cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa
    72961 gaaatgaaga ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag
    73021 gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg
    73081 atgtagtcaa attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc
    73141 atattgaagc aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc
    73201 acagcgaagt attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac
    73261 aaggaatgat cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa
    73321 tgatagacta gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat
    73381 ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg
    73441 attttaggaa cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc
    73501 caattagagg gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta
    73561 gatgaggtag tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc
    73621 gtagagaatc cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact
    73681 gatttattga cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg
    73741 ccatatgaaa agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt
    73801 atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta
    73861 gagaattcct caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac
    73921 ttagatttat ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa
    73981 cagcaaatca cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt
    74041 tttgacatga cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa
    74101 gatttagtta tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta
    74161 cgtttatttt tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct
    74221 gaaacattga aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc
    74281 gatgaacatt tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa
    74341 acgggacaaa tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg
    74401 ttaagaaatc gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg
    74461 tccttttttt gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt
    74521 tgcttaaacg agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag
    74581 ccatatcaat agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag
    74641 ctacagcatc ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt
    74701 tcacattagc gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta
    74761 gtttcacctc cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat
    74821 tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc
    74881 ttttattagg attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga
    74941 cgctattgtg ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca
    75001 aagtttgaat gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa
    75061 gttttttttg aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc
    75121 agttatttta taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt
    75181 taatgtattc atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt
    75241 tccatttgcc acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg
    75301 ccaaatagcg atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc
    75361 cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag
    75421 tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca
    75481 ttttgatatt cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat
    75541 aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc
    75601 attggcacgg cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag
    75661 catcccctat atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca
    75721 aatcaggaaa taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc
    75781 cgattgttcc tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata
    75841 tcacggatca gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat
    75901 tttcctaaag caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg
    75961 gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg
    76021 atttgtggat cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca
    76081 ccgcaaaaaa cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga
    76141 ccgatttctc ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata
    76201 ttgattcctt tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata
    76261 atcaaaacaa aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt
    76321 aaaataattc ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac
    76381 aatcataaca tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca
    76441 taagctgttt ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag
    76501 aagacagccg cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat
    76561 caggtaaagg tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt
    76621 gtcctacaac tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga
    76681 gaataacaag aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata
    76741 gccgatgact gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat
    76801 ccatagcggc atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag
    76861 gcgcatcatt gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt
    76921 tgactagttg tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt
    76981 gttcagcaat ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt
    77041 tttgggattt aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac
    77101 ctaaaacagc aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt
    77161 ccaaacgttc gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg
    77221 cgtgcttgcc aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa
    77281 tttcttctat tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa
    77341 tcgcatgagc aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca
    77401 aatccttttc atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc
    77461 ctgttttatc aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt
    77521 tgattaaaat cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag
    77581 atgcgacaag cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt
    77641 catcccaact ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca
    77701 aaatcaattt tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg
    77761 tattctgcgc ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt
    77821 ttgttactcg aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc
    77881 ctaaatattt ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct
    77941 gtgtgctttc taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt
    78001 tatccacctt tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt
    78061 gggctttttg tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg
    78121 tgtattcctc taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt
    78181 aattaccgat caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg
    78241 tccgatcatt ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa
    78301 tggcactgat ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga
    78361 agatcataaa taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg
    78421 ttattagacc tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa
    78481 cagctaaatg agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt
    78541 atttataatg attattatta aagaaaataa aaaaccagaa catttctcaa ataagagaag
    78601 tgttctggtt ttttctacat tgcaataaac caataaccaa tcaaaatgat accaaggata
    78661 atacgatacc agccaaaagc ggtaaagtcg tttttcttca aatagcttag taagaatttg
    78721 atagctaaga cggaaaccac aaaagatacg aatgttccta caagaagaac aacagtttct
    78781 tccattttga atgaattccc ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca
    78841 aacatgacag gaatccctaa gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca
    78901 atcaagatag atcctaggat cgttgcgcca gaacgggatg ttccgggaac aagtgccaag
    78961 acttggaata acccaacaag aagagctgct tggtacgtaa agtcattcag agaagtccaa
    79021 cgaggtgttt tgtttttgtt gcgtttttcg acaataataa aggcaatacc gtaaacgatc
    79081 aaaacaatgg caactggtaa gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc
    79141 aaaccaatga tgatggctgg tatgcaagca acgatcactt taaaccaaag tgtccacgta
    79201 tctttttttt ctaccgttga tttacttgga gaaaatgggt ttaacttgtg aaaataaaga
    79261 acgacgacag ccaagatcgc ccctaattgg ataacgacat tgaacatcga cataaattgt
    79321 gaactggcat ccagtttgat gaattcatct gctaggatca aatgaccagt actactgatg
    79381 ggcagccatt ccgttattcc ttcaataata ccgagaatca ctgctttgat aatatttgca
    79441 aaaaacataa attccttcct tcccaattaa gatacatctt atagtatacc gttatttttt
    79501 gagaaaccca atctgttttt taatattaag aaaaacttga gaaataaagc agataagtac
    79561 acgaagagaa aaaagaagac aaagccctcg ttcagacttt gccttttatg attctctggt
    79621 aaaaattaaa atatccccat tatagaaacg agtcgcgact atgtgttttc ccgttatcga
    79681 tataagacgt atcattgatg ccgtggatag aaaatcgacc gtttgcataa gtgacctttg
    79741 ttacgctgcc atttaaaagc ccagcacgaa caggctgttt aggatcgatc agattaagca
    79801 aaaaggcgat cgtcaaacca tgagatacga ctaaaacatt tccaccacca tttttttcgc
    79861 agtgatgggc gatatcttca aatcctgtcc atacacggtc tcgcaatgtt tcataagttt
    79921 ccgcccaccc agcggtatct aagttgtata cagcattagc aatttgttca aaagaaactt
    79981 ctgtggtaaa catttcatca tatgttttaa aattcaatac acgagggatc acaccccaca
    80041 tttccatgtc atatccacct tcaagagacc cgaaacacca ttctctaatc cttttttcaa
    80101 taaaataagg aatttctttt cccttagaat gttctcctag aataatacgg gcagtttcga
    80161 ttgcacgacc actgtcacta gaataagcaa atgaaaagtc gacatttttt aacccgcgac
    80221 ctaaataatg aatgccttct tgtccttgct ttgtgagggg tgtatcacac cagccttgta
    80281 ctcgttcaat tgtattgaac atcgtttttc cgtgtcgtac gatataaagt gtagtttctg
    80341 tcattttatt ctcctcctaa gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc
    80401 tgttcaatcg tcgttgcatc gccatgtcct ggatagactg caaattcatc aggtaataca
    80461 aacagctggg tccgaatact atgaagtaat tgttctaaat tccctgtata cagatcggtt
    80521 ctaccgatac ttcctctaaa cagggcgtcg ccagaaacga caaaatcatc aaaaataaag
    80581 ctgacgcttc ctatagaatg ccctggggta gggacgacgc gaaaagacat gtttcctaaa
    80641 gtataagttt tcatttcaaa ttcatattct gcaggcgaga caataacatc agcaatatca
    80701 tcatgtctac cgagtccaga aagattcaaa atcggatcac ctagccattt ttgctctagt
    80761 gggctgacat aaactgggat ttgataatac gcgcgtaaag gttccactgc accgatatga
    80821 tcgtagtgag tatgggtaag caaaatcgct accggctgtt ggtttgtttt tttgatttct
    80881 tgtttgattc gttccgcatc ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag
    80941 accaaataac agttttcttc aatcattcct gtttttaatc tttcgatatg catgtgcatt
    81001 cctccttgat agccaaaact tctttcatcc attagtataa cgcattcatt gctaggaaac
    81061 aaaaacaggt acttatttct ctttacttat ctgaattatc agactatatg aaaagtattt
    81121 ggatattcta aagagaagga aagtaagttt tcatagcatt ttaatatgtc cttgttacat
    81181 tagattcatc caaaaatcca aattctatat cctatatccc tgcctaatca gcagggtgtt
    81241 tttttatgtt tggaaagaga gctctttttg ctaaactagg ttttttcagg taatcttaga
    81301 gagaaagagg tggtggaaat gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa
    81361 tttattttgc gacgtggaag tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa
    81421 ggagcgagaa tccaccgcaa gctccaaaaa gatgcgggag aaaactatga aaaagaagtc
    81481 tttttaaaaa caacagttca attagatgaa tattacctga tcgttgaagg ccgtgcagac
    81541 ggtattttca aaagagacgg tatttattat atagatgaaa tcaaaacctc cgaaccacga
    81601 tttgaagatt tagaaccaga acaagtggaa cttttctttc atcaagcaag ggtgtatgcc
    81661 tatatctata gtcaagaaca tgatttacaa gaaataaatc tgcaattgac ttacttccag
    81721 acggcagaag aagtgatcac gagaaaggtt gaacatcaga caagagagca gttagaaact
    81781 ttttttaaaa agctgactga agattataaa gaatggcttg tttttcaaga aaactggcgc
    81841 actgtacgca atgcctcttt gatggctttg aaattccccc atgatgagta taggaaaggt
    81901 cagcgtgaac tagcggtagc tgcttataaa acgatccgta cgaaacagaa attgtttgta
    81961 gaagcaccta ccggaacagg aaaaacaatc tctacgttat tccccgcttt gaaagctgta
    82021 ggggaagaag agggagaaaa gatcttttat ctaacggcta aaacaataac tagacaagta
    82081 gctgaagatg ctatgacagc attaaaagat acaggagctg aagtaaaaag tgtcactttg
    82141 acggcgaaag acaagatctg ctttttagat gagacgacct gtaatccaga tcagtgccca
    82201 tatgccaatg gttattacaa tcggattaat gaaggtttat gggatctttt gaatcatgag
    82261 aatcagatca ctcgtgaagt catcgaaact tatgcaagaa agcatacact ttgtcctttc
    82321 gaactatcat tagatgtcag tatctggtgt gacgtaatca ttggggatta caattattta
    82381 tttgatccaa ctgtttattt aagacgtttt tttgaagatg aaaaaaacga ggactatctg
    82441 tttttgattg acgaagcaca taatttggtg aatcgttccc gagagatgta ttcggcagaa
    82501 ctatcttatg aaaaaacgaa acgaagtaag gaagctatac caaaggaatt caaaaaactt
    82561 catcgccgtt tcaacaaact attaaaagaa tttgattcga tccgagaaat agcaaaagaa
    82621 gatcattggg attatcatca tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat
    82681 caattaagcg aaaaaattaa agaatggctg gcagaatttc ctgagcaccc atcacaagaa
    82741 caattgcttc cttattattt tgatttgctt cattttttga aggtcagtga attttatgat
    82801 gatcactacg aaacaacagt cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt
    82861 attgatccaa gcttgtttct tgagcagtcg ttggataaag gaaaaagcag tattcttttt
    82921 tctgctagtt tttcaccact ctcttattat caggaaacac tggggggaca aaaaagtctg
    82981 gcttacaaac tgccgagtcc tttcccagaa ggaaaccagc aagttttcat cgcaaattac
    83041 ctagagacga cttatcgaaa aagggaacac agtctggcaa aattagtaga gacgattcat
    83101 gcttttgctg atggaaaggt cgggaattat ttcgttttct ttccttctta tcaatattta
    83161 gatcttgcgg tggaagcatt cagacagcaa tatcccgaca gcaatgtgct aatccaagaa
    83221 acggacatga atgaggaaga acgcgaacga tttttaacaa aatttcaaac aaatcccgct
    83281 gaaacattga ttggattttg cgtacttggc gggatttttt cagaaggaat cgatttgaga
    83341 ggggaccgat tgatcggcag tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag
    83401 caggaactga tcaaatcata ttatgatgag aaggaacacc ttggatttgc ttacgcttat
    83461 cagcttcctg gaatgaacaa agtcttgcaa gctggtggtc gagtgattcg tggcatggcg
    83521 gatcaaggaa tcgttgtatt ggcagatcaa cgattttcta gtttttctta tcgacgttta
    83581 ttcccacaac attggcaaac agcaagagaa gtacgaagtg ttggggaatt ggaagaagga
    83641 atacagcgtt tttggttatc aaaagaagca gtaaatcaaa aaaacgagga atgacagacg
    83701 tcagcctcgt tttttgatta acgatagtta gtgaattgca actcgattgg taagtcaagt
    83761 ttacgtaata aagcaatcac agcttgtaga tcatcacggt tcttacctgt cactctaatc
    83821 tgatcttctt ggatttgtgc ttttactttc agtttttcat ttttgatagc tgtagtgatt
    83881 ttcttcgcgt tttcacgatc gattccgttg accaaatcgg cagtttgtcg agctttgcca
    83941 cctagagcgt gttctgattc ggaaaaatgg atatttttga ttggcacatt tcttttgatc
    84001 agcttaccaa ataatacatc tttgatctgt tcgattttga aatcatcatc gctcaataca
    84061 actagattgc tgttctctaa tttgatttct actgtagaat ccttgaagtc aaagcgattg
    84121 gacaattctt ttgttgtttg ctgaatcgca ttttttactt cttctgtatt gacttctgat
    84181 acaatgtcga agctagcatc ttttgccata ggaaccctcc tgatatacat ataaatattt
    84241 accatacatc tatcttatca taggattagg gcagattctt acctgtttgc taaaattctt
    84301 ctcatttgag ttcgaagtat tttattttaa tagataaact gttattctta catttggaat
    84361 accaaagtag gggagaggtg cttgtgagta aaattatgac tgcgtatcat aaaaatgaaa
    84421 gattaaagaa aatagtggtt attttgataa ccggattgac cgctgcggtc gctttaaatt
    84481 attttttgat tccagccaat gtcttttctg cagggatgaa tgggatcgct caaatcattt
    84541 cctcgctttt atatgaatgg tttcatatca agatagacac aggaacattt atttttcttt
    84601 tgaatatccc tgtgtttgta ttagggtttc tcaaagtagg aaaacgagca acgatcctta
    84661 gttttgtgaa tgttgtatgt gtatcggtga tgacgatgct gcttccagtc ggacagatca
    84721 ctgataatat tttgatgaat gccctagtag gcggtgtttt attaggtctt ggcgtaggat
    84781 tatctttaaa aatgggcttt acaactggtg ggatggatat catctctctt gtactttcac
    84841 aaacaactgg aaaaacagtc ggaaactaca tgctgattct aaatggtatc attgtagtca
    84901 tcgcaggctt cttctttaac tgggaaagtg ccttgtatac gattatttcc atttatgcaa
    84961 tgactcaagt aattgatgcc atccacacga gtcatcaaaa agtaacagca atgatcgtta
    85021 ccgtcaagcc agaagaggta acagaagaac tagcaaaacg gatggtacgt ggaatgacat
    85081 tactgccgtc tgtcggtggc tattcaaaaa aacaagggaa catgatcatg atggtcatca
    85141 cccgctacga aatgtatgat cttgatcaga tcgttcatga aatcgacgac gatgcattta
    85201 tcaatatttt accaactcat tcagtatttg gccggtttgc aaatgaaaac gaacaaaaaa
    85261 tgtatcgaac aactggcgta ttgccagaaa taaaaaatcg ttcacaaaaa aaatgaggaa
    85321 tcaggtttat tttagaataa atttaatcta ttacatctaa taagaacatt tttataaatt
    85381 taaacgagaa tcttacttct aaattctcat ttacaagcag ctaaaaaaag agtatgataa
    85441 ataaaaatcg tgttaaggag tgtcgtacga tgagcgaagc aaaccaagaa cggtctggac
    85501 aagaaataga actaatcaaa gaacgtattc ttgctgcctt ggaaatggtg attgatcctg
    85561 aattaggaat cgacatcgtc aatttaggct tgatttatga tatagaattc aatcctgaaa
    85621 atggagaaac agtcattaag atgactctaa caactatggg ctgtccatta gcagatattt
    85681 taacagaatc tatccatgac gcattaaaag aagtgccaga agtaacgaaa gcagaagtca
    85741 aactagtctg gtatccagca tggacgacag ataagatgag ccgttatgca cggattgcgt
    85801 tggggatacg atagaaatct tggctgacta gctatgagaa gcaaatagga aaaacaaaaa
    85861 agccttttat cctttctaga agaaaagaga taaaaggttt ctttgtttat aaaattttgt
    85921 ctgctatatt ataagttaat taaataaatg tgttctattg ataaaggaca ttcaaatccc
    85981 tttccttttg gaatccttct tttttacatc agtttgattt tcgtatagta ttctttctca
    86041 tcttcacgat aaatcgcagc cccatttttt tgttgtgctt taagactgcc aagatttttt
    86101 ttatttacag acaaaagtgc ctcatcaatg ccgatttttt ttgcctcttg tagtactaat
    86161 gctaatcctt gtgtggcata tcctttttta cgataattag gggagatgcc gtaaccaata
    86221 tggccagcgc cgtttcttaa tgtttcgttc agtttatggc gcaaattaaa aattcctaca
    86281 tactcatgtt catcactttc taaaataaag acggttgcgg gaacgtaact ctcaggaaga
    86341 tcgattccta aagacgtttt ttcgcatttt tttacataat ctaaaaattc agcaaatgta
    86401 tagccataag cattgttgat aaatccactc tcatttggtc caaagctagt ttgtagctga
    86461 taagctttct ttaaatctgc ttcccatagt ttgatcagtt tcatcgtatt cacctcttta
    86521 agtaacctta ttcatttgtt ttctactata gcactattgt tgtaaatagc catgcctatt
    86581 tttatttatt gtgtcatatc gtattaagaa tcatcgaaca atacaaatat gaattacaat
    86641 aaaaaaatta tttgaagttc gttcattcgt tacttatgat ctctagaaga aagaaaacgt
    86701 gaattacttc agcgtctaga attccagaag gatagtaatc aacagaagaa ataatcaata
    86761 tccaaatgaa agaagatagc tgaatcgaga aaaagaggaa aaaagaaatg aagaaaaaaa
    86821 taaaatagtt actaatccca gagtcgttcg tttaacattg aaatcgatca tatggataaa
    86881 acagattttt tcaccaaccc tcaatagatg atggagttgc tcggatgaat tatttgtgta
    86941 gatggcataa gggagggcgt acagaaatga aaaagaacaa aaatatgttc ttgacaaaaa
    87001 acacatgtaa tcctaaacta aaagatgtga tgcggctatg gcggaattgg cagacgcgct
    87061 agcttcaggc gctagtgagg gtgacctcgt ggaagttcga gtcttcttag ccgcataatt
    87121 tgttttttta taaagagaag gtacacctgg agcgatgaga aagaaaataa catattatct
    87181 aaaagaatat aggaaaaatt ttaaacaacg gaaccagatt caaataaatc aaaaaaaatt
    87241 tgtgtgaagg tgggacagtt taaaacctaa tagattgatt caaatagtag atttatagat
    87301 tctgatacaa gaaatactgg caaacgatag ttaaggaata aagactaaac ttaaactcaa
    87361 aaagaggtca tgcgaaagaa taaatcgcat gacctcttac tatattttga atgtgtttaa
    87421 acgttcaaat aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc
    87481 ccaagaacca taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga
    87541 gtagtcacca tttaagtaag aagaatctaa ttggtaacgg ccgatgtaac gtccgttcgt
    87601 tgctgtgtaa gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtact
    87661 tgctggagta gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc
    87721 tgcttgtgtt tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt gaacaggttc
    87781 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
    87841 ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZKCC010000003        76026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000003 DAZKCC010000000
VERSION     DAZKCC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.003"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..661
                     /locus_tag="ACG8VH_000200"
     CDS             29..661
                     /locus_tag="ACG8VH_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108797.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(795..920)
                     /locus_tag="ACG8VH_000201"
     CDS             complement(795..920)
                     /locus_tag="ACG8VH_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP9108798.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1054..1947)
                     /locus_tag="ACG8VH_000202"
     CDS             complement(1054..1947)
                     /locus_tag="ACG8VH_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP9108799.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2129..3055)
                     /locus_tag="ACG8VH_000203"
     CDS             complement(2129..3055)
                     /locus_tag="ACG8VH_000203"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP9108800.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3140..3901)
                     /gene="pflA"
                     /locus_tag="ACG8VH_000204"
     CDS             complement(3140..3901)
                     /gene="pflA"
                     /locus_tag="ACG8VH_000204"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP9108801.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4145..6376)
                     /gene="pflB"
                     /locus_tag="ACG8VH_000205"
     CDS             complement(4145..6376)
                     /gene="pflB"
                     /locus_tag="ACG8VH_000205"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9108802.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6653..9103)
                     /gene="parC"
                     /locus_tag="ACG8VH_000206"
     CDS             complement(6653..9103)
                     /gene="parC"
                     /locus_tag="ACG8VH_000206"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9108803.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9115..11160)
                     /gene="parE"
                     /locus_tag="ACG8VH_000207"
     CDS             complement(9115..11160)
                     /gene="parE"
                     /locus_tag="ACG8VH_000207"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9108804.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11326..11760)
                     /locus_tag="ACG8VH_000208"
     CDS             complement(11326..11760)
                     /locus_tag="ACG8VH_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9108805.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11887..12525
                     /gene="plsY"
                     /locus_tag="ACG8VH_000209"
     CDS             11887..12525
                     /gene="plsY"
                     /locus_tag="ACG8VH_000209"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9108806.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12657..13532)
                     /locus_tag="ACG8VH_000210"
     CDS             complement(12657..13532)
                     /locus_tag="ACG8VH_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9108807.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13614..14405)
                     /gene="codY"
                     /locus_tag="ACG8VH_000211"
     CDS             complement(13614..14405)
                     /gene="codY"
                     /locus_tag="ACG8VH_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP9108808.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14423..15823)
                     /gene="hslU"
                     /locus_tag="ACG8VH_000212"
     CDS             complement(14423..15823)
                     /gene="hslU"
                     /locus_tag="ACG8VH_000212"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP9108809.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15837..16385)
                     /gene="hslV"
                     /locus_tag="ACG8VH_000213"
     CDS             complement(15837..16385)
                     /gene="hslV"
                     /locus_tag="ACG8VH_000213"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP9108810.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16422..17327)
                     /gene="xerC"
                     /locus_tag="ACG8VH_000214"
     CDS             complement(16422..17327)
                     /gene="xerC"
                     /locus_tag="ACG8VH_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9108811.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17525..18901)
                     /gene="trmFO"
                     /locus_tag="ACG8VH_000215"
     CDS             complement(17525..18901)
                     /gene="trmFO"
                     /locus_tag="ACG8VH_000215"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP9108812.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18870..20948)
                     /gene="topA"
                     /locus_tag="ACG8VH_000216"
     CDS             complement(18870..20948)
                     /gene="topA"
                     /locus_tag="ACG8VH_000216"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9108813.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21070..21927)
                     /gene="dprA"
                     /locus_tag="ACG8VH_000217"
     CDS             complement(21070..21927)
                     /gene="dprA"
                     /locus_tag="ACG8VH_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9108814.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21983..22750)
                     /locus_tag="ACG8VH_000218"
     CDS             complement(21983..22750)
                     /locus_tag="ACG8VH_000218"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9108815.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22743..23603)
                     /gene="ylqF"
                     /locus_tag="ACG8VH_000219"
     CDS             complement(22743..23603)
                     /gene="ylqF"
                     /locus_tag="ACG8VH_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP9108816.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23863..24261
                     /locus_tag="ACG8VH_000220"
     CDS             23863..24261
                     /locus_tag="ACG8VH_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9108817.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24498..25040
                     /locus_tag="ACG8VH_000221"
     CDS             24498..25040
                     /locus_tag="ACG8VH_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9108818.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25123..25674)
                     /gene="lepB"
                     /locus_tag="ACG8VH_000222"
     CDS             complement(25123..25674)
                     /gene="lepB"
                     /locus_tag="ACG8VH_000222"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9108819.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25826..26053)
                     /locus_tag="ACG8VH_000223"
     CDS             complement(25826..26053)
                     /locus_tag="ACG8VH_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP9108820.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26050..26568)
                     /gene="msrA"
                     /locus_tag="ACG8VH_000224"
     CDS             complement(26050..26568)
                     /gene="msrA"
                     /locus_tag="ACG8VH_000224"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9108821.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26588..27301)
                     /locus_tag="ACG8VH_000225"
     CDS             complement(26588..27301)
                     /locus_tag="ACG8VH_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP9108822.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27304..28200)
                     /locus_tag="ACG8VH_000226"
     CDS             complement(27304..28200)
                     /locus_tag="ACG8VH_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9108823.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28358..29200)
                     /locus_tag="ACG8VH_000227"
     CDS             complement(28358..29200)
                     /locus_tag="ACG8VH_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9108824.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29332..29985
                     /locus_tag="ACG8VH_000228"
     CDS             29332..29985
                     /locus_tag="ACG8VH_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9108825.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30048..30575)
                     /locus_tag="ACG8VH_000229"
     CDS             complement(30048..30575)
                     /locus_tag="ACG8VH_000229"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9108826.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30595..31542)
                     /locus_tag="ACG8VH_000230"
     CDS             complement(30595..31542)
                     /locus_tag="ACG8VH_000230"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9108827.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31554..33437)
                     /locus_tag="ACG8VH_000231"
     CDS             complement(31554..33437)
                     /locus_tag="ACG8VH_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9108828.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33600..35678)
                     /locus_tag="ACG8VH_000232"
     CDS             complement(33600..35678)
                     /locus_tag="ACG8VH_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9108829.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35691..35966)
                     /locus_tag="ACG8VH_000233"
     CDS             complement(35691..35966)
                     /locus_tag="ACG8VH_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108830.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36328..36783)
                     /locus_tag="ACG8VH_000234"
     CDS             complement(36328..36783)
                     /locus_tag="ACG8VH_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108831.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36937..38145)
                     /locus_tag="ACG8VH_000235"
     CDS             complement(36937..38145)
                     /locus_tag="ACG8VH_000235"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP9108832.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38257..38589)
                     /locus_tag="ACG8VH_000236"
     CDS             complement(38257..38589)
                     /locus_tag="ACG8VH_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9108833.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38737..39612
                     /locus_tag="ACG8VH_000237"
     CDS             38737..39612
                     /locus_tag="ACG8VH_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9108834.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39694..40275)
                     /locus_tag="ACG8VH_000238"
     CDS             complement(39694..40275)
                     /locus_tag="ACG8VH_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP9108835.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40281..41540)
                     /locus_tag="ACG8VH_000239"
     CDS             complement(40281..41540)
                     /locus_tag="ACG8VH_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9108836.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41542..42588)
                     /locus_tag="ACG8VH_000240"
     CDS             complement(41542..42588)
                     /locus_tag="ACG8VH_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108837.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42823..43098)
                     /locus_tag="ACG8VH_000241"
     CDS             complement(42823..43098)
                     /locus_tag="ACG8VH_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP9108838.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43341..44651)
                     /gene="der"
                     /locus_tag="ACG8VH_000242"
     CDS             complement(43341..44651)
                     /gene="der"
                     /locus_tag="ACG8VH_000242"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9108839.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44833..46062)
                     /gene="rpsA"
                     /locus_tag="ACG8VH_000243"
     CDS             complement(44833..46062)
                     /gene="rpsA"
                     /locus_tag="ACG8VH_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9108840.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46197..46877)
                     /gene="cmk"
                     /locus_tag="ACG8VH_000244"
     CDS             complement(46197..46877)
                     /gene="cmk"
                     /locus_tag="ACG8VH_000244"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9108841.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46926..47561)
                     /locus_tag="ACG8VH_000245"
     CDS             complement(46926..47561)
                     /locus_tag="ACG8VH_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP9108842.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47627..49030)
                     /locus_tag="ACG8VH_000246"
     CDS             complement(47627..49030)
                     /locus_tag="ACG8VH_000246"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9108843.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49017..50048)
                     /locus_tag="ACG8VH_000247"
     CDS             complement(49017..50048)
                     /locus_tag="ACG8VH_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9108844.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50079..50300
                     /locus_tag="ACG8VH_000248"
     CDS             50079..50300
                     /locus_tag="ACG8VH_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP9108845.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50465..51202)
                     /locus_tag="ACG8VH_000249"
     CDS             complement(50465..51202)
                     /locus_tag="ACG8VH_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFP9108846.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51355..52695)
                     /locus_tag="ACG8VH_000250"
     CDS             complement(51355..52695)
                     /locus_tag="ACG8VH_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9108847.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52932..54455)
                     /locus_tag="ACG8VH_000251"
     CDS             complement(52932..54455)
                     /locus_tag="ACG8VH_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9108848.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54833..55444)
                     /locus_tag="ACG8VH_000252"
     CDS             complement(54833..55444)
                     /locus_tag="ACG8VH_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9108849.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55567..55692)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55774..56490)
                     /locus_tag="ACG8VH_000253"
     CDS             complement(55774..56490)
                     /locus_tag="ACG8VH_000253"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9108850.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56496..57065)
                     /gene="scpB"
                     /locus_tag="ACG8VH_000254"
     CDS             complement(56496..57065)
                     /gene="scpB"
                     /locus_tag="ACG8VH_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP9108851.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57049..57819)
                     /locus_tag="ACG8VH_000255"
     CDS             complement(57049..57819)
                     /locus_tag="ACG8VH_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP9108852.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57816..58187)
                     /locus_tag="ACG8VH_000256"
     CDS             complement(57816..58187)
                     /locus_tag="ACG8VH_000256"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9108853.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58206..59093)
                     /gene="xerD"
                     /locus_tag="ACG8VH_000257"
     CDS             complement(58206..59093)
                     /gene="xerD"
                     /locus_tag="ACG8VH_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9108854.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59189..59662)
                     /locus_tag="ACG8VH_000258"
     CDS             complement(59189..59662)
                     /locus_tag="ACG8VH_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9108855.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59767..60630)
                     /locus_tag="ACG8VH_000259"
     CDS             complement(59767..60630)
                     /locus_tag="ACG8VH_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP9108856.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60826..62925)
                     /locus_tag="ACG8VH_000260"
     CDS             complement(60826..62925)
                     /locus_tag="ACG8VH_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP9108857.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63145..64254)
                     /gene="rpoD"
                     /locus_tag="ACG8VH_000261"
     CDS             complement(63145..64254)
                     /gene="rpoD"
                     /locus_tag="ACG8VH_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9108858.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64278..66119)
                     /gene="dnaG"
                     /locus_tag="ACG8VH_000262"
     CDS             complement(64278..66119)
                     /gene="dnaG"
                     /locus_tag="ACG8VH_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9108859.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66301..68616)
                     /locus_tag="ACG8VH_000263"
     CDS             complement(66301..68616)
                     /locus_tag="ACG8VH_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9108860.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68801..69661)
                     /locus_tag="ACG8VH_000264"
     CDS             complement(68801..69661)
                     /locus_tag="ACG8VH_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP9108861.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69720..70352)
                     /locus_tag="ACG8VH_000265"
                     /pseudo
     CDS             complement(69720..70352)
                     /locus_tag="ACG8VH_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            70519..71280
                     /locus_tag="ACG8VH_000266"
     CDS             70519..71280
                     /locus_tag="ACG8VH_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9108862.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71306..73897
                     /gene="mprF"
                     /locus_tag="ACG8VH_000267"
     CDS             71306..73897
                     /gene="mprF"
                     /locus_tag="ACG8VH_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9108863.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74085..75272)
                     /gene="tuf"
                     /locus_tag="ACG8VH_000268"
     CDS             complement(74085..75272)
                     /gene="tuf"
                     /locus_tag="ACG8VH_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9108864.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75640..75996
                     /locus_tag="ACG8VH_000269"
     CDS             75640..75996
                     /locus_tag="ACG8VH_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9108865.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        20755 a        16022 c        13051 g        26198 t
ORIGIN      
        1 atacttatca atttaatttg gagatattat ggaaattcaa cgtagaacca ctaatttttt
       61 acatttaggc tctttttttg caactttgac gatcttattt tttctatgtt attcatacct
      121 taagacctca catgcgcctt ctagtgcatt aggctggttg cttttggatc attctgatat
      181 cacagaagcc gaagaaaatc cgtttttttt gattttgtct tctctttgcg gcttactgtt
      241 ttctgtttat ttcggtgcct atttttttca tcgacatcct ggtacgttaa acgaagcttc
      301 aagcgtattg attgcattta aaacgttttc ttttttagca attctttttt attcgtttaa
      361 tcacaatacg cttttcttct ttatcatgaa tggtttagtc gtgattttat ccatttttac
      421 ttattttatt ttttcactca tttttagcca attgaaatgt ccgctcttct ttcgattagt
      481 tcctgtcacc tgttttattt acagcttgtt ttcctttttt gtccttgctt taatgccact
      541 gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg gatatgctct tcatcgtcag
      601 tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat aaaaagcatt ttgaagcctg
      661 actattacga cttcataaat gcttttttag ttttaaacac ttcaatctaa aagaataacc
      721 caagattgga taaattatta atgactcgcc attcgtttct tagtgattct ggattatcaa
      781 atggacttcc taatctaatc agctagttct tcccataaaa tgccatactt gactgaatca
      841 taatattgat tatttaaaaa tctaactttt cttatcacta cttcctttgt cattccacat
      901 ttctcaccat ttttttgcat tcctttgttt ccagaccatg tagtgaatct aataggtggt
      961 agataggcaa aatactcaaa taattctttt aaccataaag gaagagaaag gctcctcctg
     1021 caagagtcag tataattttt gcttactgct ctcttaggtt ttactcacta aaactgttcc
     1081 atattctttg atataagaaa atgcatgcgg tataaatttc ttgctcagct cagaaaagat
     1141 ttgtcgttta agaaaggcta tttcctccat tgtcaatgaa ggatccaatg ttggtaattc
     1201 aatctcaaaa ttgttttttt cgacaatagg taattctaat gtgaatgatt cttcttcctt
     1261 tatagtattc gtcacaagta gagactgatg gaaaatacta ggccggcgat ttttatattt
     1321 attcttacgt atcctgtcaa cgataacttc tatttgatca aaaaagtacg attcatttac
     1381 gtctccaata agttcggcta attctttgaa tgccttcgtt acaggaagct gattcttctg
     1441 tacaaaaaag taattagcca aagtaagcgg gaagtgttct atagaggaaa gtgtgataaa
     1501 acggtacttc tcatttcctg cagttgtcct cagcaattct gtctctcggc tgtttcttac
     1561 tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa tacgcccaaa taacttctcc
     1621 catcgctaat ttttgttcat caacagagag tctcttcaat tgttcagcta tggtttcagc
     1681 agccgaagtg gcttgttgca agtaatcttt aggatcaaat attggaaagt tcaaatgata
     1741 tcttcgttct tccctcttga ttaatcctgc atcgatcaat aaatcaagaa aatgggaaaa
     1801 tttattttgc gaaaattctt ttttcaattc tcttagaatc gtttcttgtt ctcgatgttc
     1861 atatagataa tcggtcaatg aagaaaaaag cggatcatcg gtcagttttt gataaaagtt
     1921 ttccttattt tttgaataat agttcatata tgttctcttt tctatccgtt ttattttact
     1981 taaaaggctt ttacgtaaaa aggaggaaga acaagagtca taattgactg ttattctcct
     2041 ccatttcttc ggcctttagt atgttctatg tttcttttta tatcaatcgc ttatccattc
     2101 attttgaatg cttcatacta ccatttgatt agctaaatac agccgtcaat tgaggtacga
     2161 cttgtttttt acgtgaaacg acacctttaa gcaaagcacg gttgttggtc aaagttgttt
     2221 ggaaagcttc ttctactttt tcttttcctt taccagcaac aagtaattct gaatcactgt
     2281 ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc atttgctttc atttcatctt
     2341 ccattgctgc ttctagctct gcttgtcgat ccatcacttc attaaggtcc actgtattga
     2401 cttgagcgat acgaacgttg gaactgccca ttgggaaact ttttgcatcc aaatcaagaa
     2461 gaaccgcagc tgatttatca cttagatttg ttcctgcttt caacatttca agtccataag
     2521 tgtttagatc gacttcagcg attgctgcaa gttcttttgc agctgcttca tcttcaggcg
     2581 tacatgttgg tgatttgaac aacaatgtat ctgaaatgat agctgagagc atgattcctg
     2641 cgatttcttt aggaattgtt acttggtttt ctttatatag ttttaaaata atcgtacttg
     2701 tacatccaac tggttctgca cgatagtaca atggattagc tgtttcaaag tttgcaatac
     2761 gatggtggtc tactacagct aaaatattta cttcgctgat atctgaaata ctttgttgga
     2821 attcattatg atccacaagc atgacatctg cattttctgc tttagctgac tctactactc
     2881 gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt ttcttcatta ggtgtaccta
     2941 gagcaactgc ttctgtttct ttacctaatt cattttgcaa gtgtgaaaac gcaatggctg
     3001 ccccgattgc atctgtatct ggattttggt gaccaaaaac taaaattttt gacatgtcat
     3061 tcgctccttt gtgatttctc ctatttaata atagcaaaaa aacggaaaga agcaatactt
     3121 atcttctttc cgatgcgacc tatcgcgtta aatattttgt atactcttct gtatgaagca
     3181 atcgtttagc attttcgaca cgttcttttg taggtggttc gatcccttct aatggatatt
     3241 tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg ataaggaaga atctctactc
     3301 tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg gatcaaatat tcatcataat
     3361 cacttcgctc aggaactagt acatggcgaa tccaaaccgg tttgttgatt tttgacaaat
     3421 attgagccat ttctaaaata ttttcattgt cccaatgcgt tagtttttta tgctcttcat
     3481 tatcgatgtg tttgatgtca aataacagta aatctgtata cttcatcaat tcttgaaaac
     3541 gtgaaaagaa aggttcttca tacgtaaaag gttttccgca tgtatccaat gtagtatgca
     3601 tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa atcgatctgc aatagaggtt
     3661 ctccaccact aacagtaata ccacctttat ctccccagta aggtctgaag cgttctgctt
     3721 tatctaaaag ttcatctgct gtgtattctt tccctccgcc aatattccaa gtatctggat
     3781 tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa cacgacaaag cgcagtccag
     3841 gtccgtctac tgaaccgaaa gtttcgatag aatgaacata gccgattgtt ttttcttcca
     3901 tttaagttca catcttttct ttgatttcat tttaagtaca agttagctgt cgaagcgaag
     3961 ctttctgaac aaaatttttc gcaattgccg cttcgttatg tgtaaaattt acagggacgg
     4021 aacatacaag tcctgcccct gtaaatcgtt tctttcaaat aagcatactg attattcgat
     4081 tttgatgcga tggctattca acgtcctttc gacgctaagt cgaaaaactt ttttcacgaa
     4141 ctttttacat tctgtcgtgt gaagttctca tcaagacatc taattgttgt tctcttgtca
     4201 agtcacggaa tttaactgca taaccagata cccggatcgt caagttcgga tatttcattg
     4261 gattttccat tgcatcacgc aataaatcat tactgaatac attgacattc aaatgataag
     4321 ctccttttga gaagtatcca tctaagacat tacgcaagtt ttcgattctt gtatcttgtt
     4381 cttttccaag acctgaagga ttgattgttt gggtatttga aattccatcc aatgcacagc
     4441 cgtaattcat tttagctgtt gagttcaggc tagctaataa cccactttgt tccgcattgt
     4501 aacttggatt tgctcctggc gcaagtggtt ttccagcttc acggccatca ggagtatttc
     4561 ctgtattttt accgtaaaca acatttgaag taatcgtcaa taatgaagtt gttggttttg
     4621 agttgcggta agttttatga cgtttgattt gtcccatgaa gtattccaag atccatgaaa
     4681 ctaattcatc tgcacggtca tcatcatttc cgtatttagg gaagtcgcca tccacttcaa
     4741 aatcaactgc tattcctttt tcattacgga ttgcttttac ttttgcatat ttgatcgctg
     4801 acaagctgtc tgccgcatga gagatcccag caatacctgt agcaaatgtg cgttgcaagt
     4861 cagaatccat caaagccagc tgagctgctt cataagaata tttatcatgc atataatgaa
     4921 tgatgttcaa cgtattgaca tatagttctg ctaaccaatc catcatattc atgaagttat
     4981 gtttcacttc ttcaaagtct agtatttctg atgtgattgg acgatattct ggtccaactt
     5041 gggcgccagt cacttcatcg actccgccat tgatggcata taatagtgtt ttagccaagt
     5101 tagcacgagc gccaaagaat tgcatgtctt ttccgacaac tgttcctgat acacagcaag
     5161 caatcgcaca atcatcagaa ccccattgtt cacgaagctg attatcattt tcgaattgaa
     5221 tcgaagaact ttcgatagcg attctgctag catatgttct gaatccttct ggaaggtgtt
     5281 cagaatacaa aacagtcaag tttggttctg gtgaaggtcc catatttgtt aatgtatgaa
     5341 gcagacggaa atcattcttt gtaactaatg aacgaccatc taatcccata cctgcaatgg
     5401 ataaagtagc ccagataggg aatcctgaga ataattggtt atattcaggt gtacgcgcga
     5461 atttgaccat acgtaatttc attactaagt ggtcgatcaa ttcttgtgct tctttttctg
     5521 tgattactcc attatccaaa tcacgttgga tatagatatc aaggaatgct gagatacgtc
     5581 cgattgacat cgcagcacca ttttgtgatt tgattgctgc taaatatccg aagtatagcc
     5641 attggacagc ttcttttgca tttgcagctg gtttagaaat atcatagcca tatttcgcag
     5701 ccatttcttt catttgcgct aatgcacgat attgttcagt gatttcttca cgtaaacgaa
     5761 tcacttcttc actcattgta tgataaccag tcatatcatg atcttttttc ttttgttcca
     5821 tcaaatagtc gattccatac aatgccacac gacgatagtc accgatgata cgtccgcgac
     5881 cgtaagcgtc tggaagccct gtaatgattt tgtttttacg agcagcacgc atttcagcag
     5941 tataagcgtc aaatacacct tgattatgtg ttttgcgcca gtcagtgaaa atatgtgtca
     6001 attcatcgtt tggtgtatag ccattgcttt ttagcgcatt gttagccata ttgattccgc
     6061 cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg tagaccgacg attttttcca
     6121 aatctttatc caaatagcct gcatcgtgtg aagtaatagt tgatacgatt tctgtgtcca
     6181 tatcatagac accgttacgc tcgaattgga tatcatttaa cttttgcaat tcatcccata
     6241 atttttctgt tgcttctgtt gggccttcca aaaagctgtc atcaccgcga tattcagtgt
     6301 agttattttg aataaagtct ctagtgtcta cgccttcttt ccatttggta cctttaaatc
     6361 cattccattg ctccattgta tggacctcct aaatttctgt tttatgtaac agtttttacg
     6421 tgattagtat aacacagcac cctatctttg caagggtttt cttgtttttt atttaacaat
     6481 ttttatgttt tatttacata tatttaaatc aacgttgaca taccaacatt taagcattgt
     6541 gaacactttc atcttttttt gcaaacgtat aaggatttat taaaaatgaa tgaaaaagag
     6601 acgccacctg tattaataca gatggcgtct cttcattaaa atactacaac agttacgact
     6661 cgatgaaaat ctcaggagca tcgataactt cgatgacttc tccacctttt ttctcatcca
     6721 tcgcaaatga tccattcgat gttcgatcac ctatagggaa atcagttggg tcaatcgtta
     6781 actgctgccc tttttgattc atcaaggtta tttcttttga tcttccttcc gacatataga
     6841 taatacggtg cggatttttt ttcagctcac gcatgaccgt cactccccgc ttcgcacgtg
     6901 aagtttggct gatttcttga gcaagcatgc gtttaacagc tcctcgctga gtaacgatta
     6961 cgatcggcgt atcaccttct gctaacacaa gcagcccgtt gataatccta tctccatctt
     7021 tcaaattcat agacttcact ccagctgcct ttgctccaac aacgggaaca tctgttaaag
     7081 gatagcgcaa acccattcct tgatgcgtta ctaagaaaac atctaagcct ggctgatctt
     7141 ctttcagata cacagcaatc agttcatctt cttccgcctt tagcttcata cagctgagcg
     7201 gacggctctt ataggtgcgc catggttcga actctgtcat tcgtgtttgc ttgatcaatc
     7261 cattcttgct gatgaataca aatgttttct ctgcttgaat tttttgatat gggaaaacag
     7321 ctaaaatcga ttcgtctaca gacagattgg agattgtttg agaaatgtgc tcaccagcat
     7381 ctttccattt caaatcgggt agttcatgga cagggcggta gatcacattt gctttatttg
     7441 tgatcagaag aacatggtcc aatgtactca attcacctga gtatagcaag tagtcgcctt
     7501 ctcgcatccc gatttcttct ggtttggaag cactataaga acgaaggctt gttcgcttca
     7561 catagccttc tcgcgtcaca ctgacaatta cgtcttcttg tgccactaag acttgcgtat
     7621 caattttgat ttcttcgatt tcgtcttcga ttgtcgttag acgagcggaa gaataatttt
     7681 ttttcacttc tcgcaattct tttttcataa cagagaaaag ctctttgtca tttgaaagga
     7741 ttttattcaa ctctgtgatt tgcgcaatca agctttcgga ttccttttga agctctgtga
     7801 tatccgtgtt cgtcaaacgg tataattgca atgtgacaat tgcttctgct tgttcttctg
     7861 taaattgaaa gacgtcgact aaattctttt tggcgtcttt tttgtcttta ctttcacgaa
     7921 tcgttgcgat tacttcatcc agaatcgata aagctttcat caatccttct acgatatgct
     7981 gtcttttttg agctttggct aaatcgaatt gactacgttt tgttatgact tgctttcgat
     8041 gctcgatata acttcttaaa atgtctttca gtccaacttg atgcggagtc atatggtcga
     8101 tagcgaccat attgaaattg taattgatct gtagctcagt gtttttgaac aaataattca
     8161 aaattccttg agcattggcg tcttttttca attcgacgac aatttgcaga cctgttctat
     8221 cactttcatc tcgtacttca gctattccat cgattttctt attcaaacgg atctcatcca
     8281 tttttttcac caaggtcgct ttatttactt catatgggat ttctgtgatc acgatttgct
     8341 gtttattacc cttcattgcc tctatctttg ttttcgaacg aagaataact tttcctcgtc
     8401 ctgtttcata agcttttttg atttcatctt ttccttgcaa gatccctcct gttggaaagt
     8461 caggtccagg aatatattcc atcaattttt caaggctcgc ttgtggatga tcgatcatat
     8521 agattgtgcc atcgatcact tcagctaagt tatgagtcgg gatttctgtt gcatatccag
     8581 cagatatacc tgtcgatcca ttgacgagaa ggtttgggta tcgagctgga aggactgttg
     8641 gttctttttc tgtatcatca aaattccaca cgagatcgac tgtttctttc tcgatatctg
     8701 ctagcatctc tccacttaac ttggataatc tggcttcagt ataacgcatc gcagcaggtg
     8761 gatcaccgtc catactcccg ttattcccgt gcatttcgat cagtacttcc cgcaacttcc
     8821 agtcttggct catgcggacc atcgcttcat aaatactgat gtcaccatga ggatgataat
     8881 tccccatgat atttccgaca gattttgctg atttacggaa tcctttgtca tacgtattgc
     8941 catctttgtt catggcaaat aatatccggc gttgcactgg cttcaatcca tcacggatat
     9001 caggcagtgc acgttcttgg ataatatatt tggaataacg tccgaagcgg tcacccatca
     9061 cttcttctaa agttaattct tgtacttctt gtcgattttc catcaggtga cctcctattc
     9121 tacttcaaat aactgattgt tttcattttt atctgctcgt tcttcatcta ataaatcatt
     9181 agaaactgaa ggagagattt cagtatcctc ttttttatct aagatactgc catcttcttc
     9241 gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga ggttctactt tgtcacccat
     9301 cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg atacggacgc ggatcaacgt
     9361 acgggaagtc ggatccatcg ttgtttccca caattgttcc gcattcatct ctccaagtcc
     9421 tttatatcgc tgcagcatat atcctttgcc gactttttta atcattgctg ccaattcgtc
     9481 atctgtccac gcatattcaa tgacttgctt ctttccttgt cctttggata ctttatataa
     9541 aggcggtaac gcgatatata ctttcccagc ttcaatcagc ggtttcatat aacgataaaa
     9601 gaaagtaagc agtaatactt gaatatgggc gccgtctgta tccgcatcgg tcatgatgat
     9661 tactttgtcg taattacagt cttcgataga aaactcagga ccgactcctg caccaatcgt
     9721 atagatcatc gtattgattt cttcattttt caagatatct tgcattttag ctttttcggt
     9781 attgatgact tttcctcgta aaggtagaat tgcttggaac tttctgtctc ttccttgttt
     9841 agcagatccg ccagcagaat ccccttcgac taaatacaat tcatttttct tagggtttcg
     9901 ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg ccttttttac gcttcttccc
     9961 atttcggctt tcttctctgg ctttacgtgc tgcttctctc gcttctctgg ctttgattgc
    10021 tttacggacc aacatctggc tcatttcgct attttcctgc aaataaaagc ccatttgttc
    10081 actgatgacg ttatctacta cggtacgagc aactggcgta ccaagttttt cttttgtttg
    10141 cccttcaaat tgcagaagat tttcaggtac acggatagaa agtacagcag ctaacccttc
    10201 tctgaaatca ctaccttcca gattcttgtc ccgttctttc agcaaaccaa cttttctcgc
    10261 atattcatta taagatttcg tcatcgccgc tttcatacca gcttcatggg ttcccccatc
    10321 ttttgtccga acgttattga caaaagataa aacattttct gaataaccgt cattgtattg
    10381 ataagcgacc tctacttcga taccttcttt ttctcctgaa aaataaacaa ctggtgtcaa
    10441 tgtatctttt tcttcattca gataatcaac aaattctttg attccttctt cgtaatgaaa
    10501 aatttccttg actggttctt ctccacgaag atcagaaaga gagattttaa cgccttttag
    10561 aagaaaggca gattctctta accgttcggc taaaatttca tatgaaaatt tcgttgtact
    10621 gaatattgta tcatctggta aaaaatggac agtcgtcccg tttgcttttt ttgtttttcc
    10681 tatctttttc aaagtaccgt caggtttacc gccgttttta aatttctgct gatactctac
    10741 gccatctcta acgatcgtta caattagcca tttcgataag gcatttacca cactggctcc
    10801 tacaccgtgc agtccaccag aagttttgta ccctccttga ccaaactttc cgccagcatg
    10861 cagcacagta aaaatcactt caactgttgg tattccggaa gcatgcattc ctacaggcat
    10921 cccacgtccg ctatctgcaa cagttatact attgtcttca tggatggtca catcaatctc
    10981 actgccataa cctgataatg cttcgtcaac tgcattatcg acgatttcat agactaaatg
    11041 atgcaagcct cggctgtcag tggatccaat gtacatccct ggtctttttc taactgcctc
    11101 taatccttcc aatacttgga tggacgagtc attgtattcg ttgtttactt tttttgccaa
    11161 ggcaaacgct ccttcaataa tactttgcgg caaaaccgca ccacgattat catactagaa
    11221 aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt taattatctc ctaaccgctt
    11281 tttcgacatt cccctctatc gatcatctct tctttgtggt catttttatt tttggttttc
    11341 tttcaactta ccaagttcaa tcttaataca acgatccatt acgatatctg tacgtcctgc
    11401 ttttcttagc atctcagctg cttctttgtt ttcaatccct aattgtgccc agaacacttt
    11461 agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa aattcactcc gtctaaatac
    11521 atctacgata tcgattggat catcgatttc tgtcaaattc gcatagactt tctcgcctag
    11581 aatttcttca ccagccaata atggatttac tggtatgatt ttatatccat tgtcttgcaa
    11641 tacctgtgca accatgtaac ttgttcgttc tggtttattg ctcaagccta cgacggcaat
    11701 attttttgct tcttccaaat agttgaaaat cgtttcttgt gttggatttt ttacagacat
    11761 ttctgccacc ccttgctttt ctttttattc catctattat acttgtatct ttcatttttt
    11821 caattgttcc tgttacctat tcatgctaaa ataaaggata gttttcaact tgaaagaggg
    11881 taaaaaatga aattgatctt attacttatt ttagcatatc ttttaggttc tattccttcc
    11941 ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc gacaacacgg gagcggtaat
    12001 actggaacga ccaatacatt tcgtgtatta ggcaaaacag ccgggattgt tgtcctgctt
    12061 atggatattt taaaaggtac attggctact tgtttgccga tgatcttcca cctttctggg
    12121 attaaccctc tttggtttgg ggtatgcgcc attcttggcc atacatttcc gatttttgct
    12181 gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa tgcttttagg atttaatcct
    12241 gtattttttg tttattcttg tatcatcttt atcgttagtt tattttgtac aagtatggtg
    12301 agtttgagca gtatgatcag tgcagtattg atcactttat ctacgatcgt ccttccttat
    12361 gtagctcctg tgattttggc taagcccaat tggttactga cgatcattgc ctttttagtt
    12421 tcttccttta tcttttatcg gcataaagac aatatcaagc gtatcagaaa ccatactgaa
    12481 agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat cctgatactt tttcattcca
    12541 aagagataaa acgggagtgt gacaaatcga tgtcacactc ccttattact tacaggatag
    12601 cttttttaca caccttggta aacggtgttc cctgctaact tctcggtcac aacctcttac
    12661 ttcactgtga tggaatattt tgtcttgaag atttcatggg cacctagttg attgatccct
    12721 actttttcca tcaagtttcc actactgttc accgtgtcag cgattccgca ccacggttcg
    12781 atacaaacga aaggcgcttc ttttggataa ggagaccaga tccctacata aggcatatct
    12841 ttatagctta aagttacgct atgggaagat ttttcacttc gaatggcgta cgcatttaga
    12901 ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt cacgattcaa agccagattc
    12961 gtatttgtct gacctaacgt cttttgatcc atatcgatga atgggccttt caatggtagg
    13021 cgtaatcttg atttcaatgg agaaaatgcc agatagaaat cttcaaatga caatccttct
    13081 tcaagaggta cattgaaagc aggatgtcca ccgatagaaa agaacatttc tttttcaccg
    13141 gtatttgcca cttgataatg aaccgtgatg ccgtctcctc ctaattcata gctgatcact
    13201 aattcaaaat caaatggata cttttctaat gtctccggcg tactccttaa gatgaaacta
    13261 gccatttctt gtccatgttc aatgacttca aaatccatat cccgagcaaa tccgtgttgc
    13321 cccatttgat atgttttccc ttgataaaca tactgatcat cttttaaacg tccaacaaaa
    13381 gggaataaaa ccggtgcatg tctgccccaa tacgctggat caccttgcca gatatactct
    13441 aaaccatgtt cttttgattt cagactgatc aattccgcac cgtgttcagc gatttcggcg
    13501 atcagctggt cattttgaat aataacagtc attgccattc ctccaacttc gacttacttt
    13561 ttaaaatggg aaggctggaa caaacatttc tatctgcccc agtcccctta cttctaataa
    13621 ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata cttttaaata agttcccttc
    13681 atgcctaacg aacgagattc aatgattcca gctgattcca attttcgtaa agcattgacg
    13741 atcacagaac gagtgatgcc gatactatcg gcaatattag aagcagtcag acgaccttca
    13801 tctccatcca atgcttcaaa gatagcttgt acagccttca attcgctata agataatgtg
    13861 ttgattgcca tttggacagc cgttgcactc cgtacatctg cttcgatgct tcgagacttc
    13921 tgataaagga tctgcatacc gacaaccgta gctccatatt ctgctaagac tagatcgtca
    13981 tctgcaaacg attgttccat acgagataag ataatcgtac ccaaacgttc gccagctcca
    14041 aagatcggaa cgacagtcgt cagaccaaat ggatatttct ctcttgactc taccggaaat
    14101 gcagtaatat cactagtaat tgggatatta gcttcagttt tcatcaactg tgccactgta
    14161 tcagtataag attcaggaaa ttgtttctca acaaacattg ttttgattcg ttcattgttc
    14221 acatcgtgac gttcattata acctagtaat actccgtcac ttcctatgat ataggtgttg
    14281 ctgtcaagaa taccacctag tgtgacagcc attttatcgt aaggcaaatc tgcttgcatg
    14341 tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc gggttttcgt tagcaagtct
    14401 gtcattgtcg tcctccgatt ttttataaaa tatagcgact taaatcttca tttgccgcaa
    14461 ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt tatttctccc atttgcatat
    14521 ctggtgcttc aaataataga tcttctagta gtttttccaa aatggtatgc agtctgcgtg
    14581 cgccgatatt atctgtgtca cggttgacat cgaaagcgat ttgagcgatc cgttcaattg
    14641 cttcttttgt aaacgtaact gacacatttt ctgtaccgat caacgcgacg tattgtttta
    14701 ctaaagcatt atttggttcg gtcagaatct tcacaaaatc ttcagctgtc aaatcatcta
    14761 attctacccg tatcgggaag cgtccttgaa gttctggaat tagatcactt ggtttagaca
    14821 aatggaaagc tcctgaagca atgaatagga cgtgatctgt ttggatgatc ccgtattttg
    14881 tgtttacttg tgaaccttcg acaattggca atatgtctct ttggacgcct tctctggata
    14941 cttctccaga ttgctgtgat ttagaagtga tcttatcgat ttcatcgata aaaataattc
    15001 cactttcttc cgctaatttg attgcctcac tatggatatc agcatccttg actaattttg
    15061 ctgattcttc cttgatcaat aactctaaag cttctttgac tgttaccgtc cgttccactt
    15121 ttttcttcgg tgtcaaagca cctagagttt catttaggtc gatccccatt tgctccaatc
    15181 cattgttcat cattggtgca ggtttctttt ggtcttcaac ttcaatggtt acttcacgac
    15241 tatccagtaa acccttttgt agttgttcca agactgtacg gcgattgatt cgaatatctt
    15301 ctgtcacttc ttcttgcggt tcagcggtat tttgctgact agcattaaaa agattcatca
    15361 tttgttcaaa ttgattagtg gattgtttct gttctttttt gataccaggt accaagattt
    15421 ttaccaaacg tttttctgca ttttttttag cacttgtgta tacacggctg tactgctctt
    15481 tttctacgat ttggatcgca ttttctacta aatcccgaac cattgattct acatctcttc
    15541 cgacataacc gacttcagta aattttgttg cttcgacttt gacaaatggt gcattgacga
    15601 tttttgccaa acgtcgtgcg atttctgttt taccgacccc tgtaggtccg atcatcagca
    15661 tattttttgg tgtaatatct tgttgcattt tttcatctaa ctgcaaacgg cgatagcggt
    15721 tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg tcctacgata tattcatcta
    15781 attcacttac gatttgtctt ggtgtttgtt ttaattcata catatctact cacctcttac
    15841 agttcttcta cgataatatt atgattagta aagatacaga tatctgcggc aatatttaac
    15901 gcattttctg caatttcttt cgcactcatg tcgttttgag catagttttt cattgctcgg
    15961 gctgcagcca acgcaaaatt ccctcctgat ccgatagcta agataccatc atctggtgtg
    16021 ataacttctc ctgtacctga aacaagaagc atttctttat cattcattac gatcagcatc
    16081 gcttcaagtt tttgcataga ttgctgtgtc cgccattctt gagctaattc gacagctgcg
    16141 cgttgtagat tcccgttata ttcgtttagt ttgccttcaa atttttcttc taaagtaaac
    16201 gcatctgcaa cacttccggc gaaacctaca acaacttctc cattgtagat acgtcgtact
    16261 ttctttgcag tgcctttcat gacaacagat tcacccattg ttacttggcc atctccagcc
    16321 atagcaaatt tcccgtcttt ttcgacggca cagatagtgg ttgaatgaaa tggtgattcg
    16381 accatgtttg tttcctccta aatttgttct tttccttatt tttacgctct tggatggaaa
    16441 gaacgatagt ttttttgtaa actttctttt gttacatggg catagatctg ggtagtcgat
    16501 aaattggcat gtcctagcaa ttcttggact gtccgcaaat ctgccccatt atttaataaa
    16561 tgagtagcaa atgtatggcg cagcatgtga ggatggatat cactatccaa actgcttttt
    16621 ttgatcattt gattcaagac atattcaatt ccagtaggcg tgattggttt tccatgatga
    16681 ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca tcaatacctg gcggccttcc
    16741 ctgaaatatt cttgcagagc atcttgagca aaagaaccta gaggagcata tctctctttg
    16801 ttccctttac catggatcaa catgacctct gaatcaaaat caatgtctga taccgacaaa
    16861 ctgctacact cacttaaacg gatgcctgac ccgtaaagga cttccagtaa agcgcggttc
    16921 ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa gtgcttgcat ttcattttca
    16981 taaaaaaaac gtggcagtct caaatttttc tttttgagat gaacgtaaga aaatggattt
    17041 tcttttatca cttcttcttt gagaagatac tgataaaacg aacgtaggct ggctatcttg
    17101 cgactgatag aatttctgct atagctagga cgatgatcag ctaatgcact caaatagaca
    17161 cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc cagattcctc caaaaaatgt
    17221 ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt tttctgaata tcctctttcg
    17281 acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt ctgtcatttt ctcaccttcc
    17341 ttttgtccca gagaataatt tagcacagcg tttataaaaa gacaaataaa ttgtcagaat
    17401 ttaaccaata tttctaagtt tgttcacaat tggcgcaatt cctcacattt tgtttgagaa
    17461 attcttaaga aaaacgcaag aaaaagagcg agactatccg tctcaccctc ttcatggagt
    17521 ttccttactc atttgggatt gcagtttttt ctaattctgt ttttattgcc tcattagctt
    17581 ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt gtctcgaatg cgctctggca
    17641 gttcaggaaa cagaccatag ttggcattca tcggttggaa atgttttcct tccgcatgag
    17701 taatgtagta agccatactt ccaatagctg tttcttgtgg aaactcgact ggttccattt
    17761 cttttgctaa gcgtgcggcg ttgattccag caagtagtcc gctcgctgca ctttcaacat
    17821 agccttctac accagtcatc tgacctgcaa agaacagatc ttcgcgtttc gttgattggt
    17881 aagtcggttt cagcaattct ggcgaattca taaagctgtt tcggtgcatc acaccatagc
    17941 gaacgaattc agcattttct aacccaggaa tcatccggaa aacacgtttt tgctcgcccc
    18001 atttcaagtg agtctgaaag cccacgatgt tgtataaaga agctgcagca ttgtcttgac
    18061 gcagctggat aacagcataa ggacgtttgc cagttttagg atcttccaat ccaactggtt
    18121 tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc cattacttca attggcatac
    18181 atccttcaaa atatttttct ttctcaaaag atttaagttc tgccacttcg gcattgatta
    18241 gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg acagttcaag taagctgctt
    18301 ctcctttgtc atagcgggac ttcaaatata ctttatccat atcgatcgta gctttgtcta
    18361 cgattggtgc agctgcatca tagaaataaa aaccatctga accattgaat gctttgatgc
    18421 tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac tacggtgatt ccttccggta
    18481 tttcagtaat ttcttcgttt atcactgtta ctaatggatg ctctttgact tttcgggtaa
    18541 ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc accagctggc actgctgttt
    18601 catctgccga agtgattact accgaattca atcggcgcat ttcttctttc aataacccta
    18661 ctgcattagc caaactgttt cctcttaatg aattggaaca tactagttca gcaaaattac
    18721 ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc atataaacgg acagggactc
    18781 ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag ccctgctcca acaacattta
    18841 ccattttcaa tgaaagaacc tcttttctat tatttttgga tgtcttcttc gtaatctcca
    18901 tttacacaga ctacttgttt accgcctttg actttcttct cgaccagata tccgtcacat
    18961 tttggacaat ttctgcccac tggtttatcc caagaagtga attcacattc tgggaaacgg
    19021 gaacaaccat agaatagtct gtttttctta gattttcgtt caatgacttg tccttcatga
    19081 catactgggc aagtcacacc gatttctttt acgattggtt tcgtattccg acaatcagga
    19141 aaattactgc aagcataaaa cttcccatat ttccctaatt ttatcaccat cggatgtcca
    19201 cagacatcac aatcgaatcc tgctggttca tctttgattt gaattttttc gattttttct
    19261 tccgcattcg tcagttcttt ttcaaacggt ttatagaaac gatcaaccac tttgacccac
    19321 gcttccgtgc cttcttcaat tttatccaaa tctccttcca tttctgctgt gaaatggata
    19381 tcgacgattt gagggaagaa ttcacagatt aatgaattga caatttctcc tagttcagtc
    19441 ggctcaaatc ttttttgtgt caattttaca tagtagcgtc tttggatcgt atcaagtgtt
    19501 ggtgcataag tggaaggtcg ccctaccccg ttttcttcta gtgcacgaat caacgtagcc
    19561 tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg gttcaatatc aacagattgg
    19621 actacatcgc cttcttcaag ttctggaaga atattttctt tctcttcttt gccgtcatct
    19681 ctaccttcca catacacttg catgaatcct ttgaatttga tttttgaacc gttcgcaatg
    19741 aagatgacac cattttgttc taatgtcact ttcattgtat ctaaaatagc tggtgtcatc
    19801 tgactagcca caaaacgtga ccagatcaat gtataaagtt tcaattgatc tttatctaaa
    19861 tattgtttga gttcatctgg tgtacgaagc acactcgttg gacgcacagc ttcatgggca
    19921 tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat gtctagaaaa ttcttttcca
    19981 tacgtatctt caataaatgc tgcggcctct gcttttgctg tatcggcaat ccgggtagaa
    20041 tcagtacgca tgtatgtaat caagccgact gttccttgtt tacccaaagt gattccttca
    20101 tataattgct gtgctaccat catggttttt cttgttcgaa agtttaactt acgggcagct
    20161 tcttgttgca ggctacttgt agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt
    20221 tctacttttt taacttcgaa gtctgttcca tcaattctag cagtgacttc ttttacactt
    20281 tgtgcatctg gtaatttctt tttcttaccg tcgattcccc agaaatttgc tttgaatttc
    20341 ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt cttctggaac aaattctcgg
    20401 atctcttttt cacggtcaat aataattttt aaagcgacag attggacacg tcctgcactt
    20461 aaccctttct tgaccttacg ccagagaata ggactgatcg aataaccaac gattctgtct
    20521 aaggtacgac gagcttgctg tgcatctact aaatctacat cgatcgttct tggttcctta
    20581 aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga ccacccgatt tttttctttc
    20641 agatccaacc caagtaggta cgctaaatgc caagcaatcg cttctccttc tctatccggg
    20701 tcacttgcca agtaaacttt ttgagctttt tttgctgcgg ctttcaggct tttgatgaca
    20761 tcgccttttc cgcgtataga aatataatgg ggttcataat tgttttcaaa atcgataccc
    20821 attttacttt tcggtaaatc tcttatatgg ccaacactgg ccattacttt atagtttcgt
    20881 ccaagatatt tctcaattgt cttggctttt gcaggtgatt ctacaatcac taaatattta
    20941 tacgccatta ctgaatggac tcctttccat tgtcgtgttc ctactattaa aagaggattc
    21001 atacaaatcg tagctaatca tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa
    21061 agtatggtgt tattctttaa attctgccaa gatatcttca cccgaaaccg tgcaaactgc
    21121 accgtcttgg atcaaatgat gacatccggt agaatgttct gttaaaatat ttcctggaac
    21181 agaaaaaact gttcttccaa gatccaatgc ttgctgtgct gttatcagag agccgctttt
    21241 tttcttggct tcaaagacac atgttccttt agacaaagct gcgataatcc gatttctcat
    21301 tggaaaacaa tgctttttcg ggccggattg aggaggatat tcactcaaaa gaagatgatc
    21361 atttgcaatc acctcttgca agtcacgatt ttcagcagga tagacttgct ctatccctgt
    21421 tccaatgact gcgattgtcc gtccaccatg agagattgct gcttggtgac cgtatgtatc
    21481 tatccctttc gcgcagccac tgacgatcac atatccagct tctatcactg gttttagtaa
    21541 ataataaact gtttttttgg ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat
    21601 gttacaagaa agtaaagata gatcaccatt ataaaacagt acaattggcg gataggtcaa
    21661 gtgaagcagc tgagggggat atttgggatc aaagcaagtg ataaacgact gtttcgattg
    21721 gattttgccc cagttagcgt gaatttcctg ccaacttttg cgaaacagct cccggtgttt
    21781 ggttatttta cctatcttaa tgatgtcttc aaatccgatt tctttataag gatactgcat
    21841 catccagtta gcaagtgccc attttccttg actactgatt ccttttgtat aagcaagttt
    21901 caataaattt tcttctattt gatacatcat aaaaccgcct ttcgtctctc tttctaataa
    21961 gatacgtaaa agcggctgtt ttttattgta aatatttttt tacaggtgca aaacttttac
    22021 gatggattgg cgtaattcct tgtcgttcaa gtccttctaa gtgttccttt gttccataac
    22081 ctgcattatg cgagaaacca tatccaggat aaagcacgtc atattctttc atcaattggt
    22141 ctcgatatac tttagcaatg atacttgcag cagcaatcga cacagatcgt gcatcccctt
    22201 tgattatttt ttcctgctga ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc
    22261 ttggtacaat ggacaatttt tctactgctt ggatcatcgc taatttactt gcttcataaa
    22321 tattcacatc atcgattgtc tgctgatcaa cgatcccgat cccgattgcc agggcttttt
    22381 cttgaacttg ttgataaaga agttctcgct ttttctctga taattgtttg gagtcattca
    22441 gccctaatat gatctcattt ttcggcaaga taactgcagc tgccacgaca ggtccagcta
    22501 atggtcctct tcctacttca tcaatgccgg caatccattg gtgaccttgt gcgtaccctt
    22561 gcttttcaaa gcaaaacatt tcttcatatt tttccttaat taatgcttcc tttttcaatc
    22621 gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg tggatcattt cttaatgatt
    22681 ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg ttctttgata ctttggatgg
    22741 attcagtcat cacttttttg ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt
    22801 tttccttgac gaatatcatg cacgactaat tcgctgccac gatcatagtc ttctttatac
    22861 ccacgttttg ctgtaatatc catcagtaat tccggtgcca atttttcttc ttgctcgatt
    22921 gataaaccat aacgatcgct gattcttcct gaataatatc gggcaaaaaa gtctaatccg
    22981 taaatcgcta aatcatctaa atggacaagc tggtctttga tcgcgcctgt cagcgctagt
    23041 tttttgccga tttcttcatc ttcaaatttg ggccataaaa tccctggtgt gtctaacaat
    23101 tccaactcat tacctaaacg aagccactgc tgaccttttg ttacgccagg cttatttcct
    23161 gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg tagatttccc aacatttgga
    23221 atacctaaga ccattgcccg aatagcgcga ggcttgatcc cccgagcagc atctcttgcc
    23281 cgtttttctt tcaatgcttc tttagccgca ggtactagac ctttaattcc tttactttct
    23341 tgtgccgtga tagctaatgc aagatggcct tgtttacgaa aatattcttg ccacagcttt
    23401 gtctgttgtg gatctgccag atccgcttta ttcaataaaa caaggcgagg tttttgctga
    23461 atgatctgat ctagcattgg attacgagaa gataaaggta atcgtgcgtc taccaattca
    23521 aatacgatgt ccacatattt cattttttca gaaacttctc gtcgggcttt tgccatatgt
    23581 cccggaaacc attggatggt cattgttctc tccattcctt tcataccgga atattccccg
    23641 gctttacttg tttttttctt tttcaatata aattcacttt tttattataa agaaattcca
    23701 tctattagtt aagtgaaagt gaaccagtcc tcattctatc acatcttcac ttttttgatg
    23761 atgcaaattt ttattttggt ttctatatat gaatctaaat ttttgacagc gtttcctaaa
    23821 taagagatga tagacgaaca aaacagaaag gagacgaaac aaatgaatag tatggttttt
    23881 gttaattttc ctgttgctga tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt
    23941 aaaaaaaatg aagaattttc tactgaagaa gctggttcaa tggtatggga tgacactttt
    24001 tggataatgc ttctctctca tgatttttat aaaaaattta taaaagataa agaaatagct
    24061 gataataaaa aaacaagtgg tgctttgatt tcgtttagtg ttgaaaatgc ggatgaagta
    24121 aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt tccacgaagt agatatggga
    24181 atgcctgaag aagaaatgtt ttcattagaa gtccaagatc tagacggaaa tacattagaa
    24241 cccgtatgga tgaagatgta agcagataac taatctgacg ttttttataa caaaaaaaca
    24301 aaggcggatg atacgataag tattctccgc ctctattttt tttattcgta caatctcttt
    24361 tttacagttt ataaaagcag tcttctcacc ctttttttaa ttgttagatg ccagtgtatg
    24421 taaatcacca atgtgtcatg acacattggt gattttatgg tatcctttta ttattaactg
    24481 acaaaggaga gaatattttg aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa
    24541 tgaatgcaag attgttgtcg acctctgctc ttttattagc ccttgatgtc atcttataca
    24601 agatagcgat tggtcctgct tattttcaaa tcacattcgg atttttaagt atggcgctta
    24661 tgggttatct atatggaccg atctgggctg gtttgcttga aatgttgtcg aacactttaa
    24721 atttcacgat atttggttca ggaacgtttc aagctgcttt tcttatcacg gctttccttg
    24781 gtggatttat taatggctta tttttatatc ataaaaagat aaacctgcct tccgtcatcg
    24841 ctgcccaatt attgattatg atcccagtca gcctttttat caattcttgg ctgatgtctg
    24901 ttttgtttgg tgcagatttc aaagtaattt ttctaggtag attcatcagg aatatgatcc
    24961 aaatccctat tcaaataatg gttgtttatt catttatcaa aactttagag gctagaaaag
    25021 tgtttaaacg agctgtttga ttttacgtac taaggtagta aactgatttt ggattaaaca
    25081 ataaagcgtg cagtctcaac agactgcacg ctttattgtt tctcactgga tccattttat
    25141 ttcatcaagt ggataataga caaatcttgc tttacctaga atatcagttt tattgatcgt
    25201 gccaaacgaa cgactatctt ttgaaactct tcgattatct ccaagtacaa aataattatc
    25261 ttccggaagc ttggattgtc ccgttagctc ttgtagtgta aagtccgtcg tataggatgc
    25321 atttgcatga tctgatttca ggtttttagt tagataaggt tccttaacta ctttcccatt
    25381 gatcttcaat tgatcatttt ggtagctgac attttctcct ggaagaccga ttactcgttt
    25441 gatataaatc gtgccatctg ctaattgaaa aacaacaata tcaaatcgtc tgatctccga
    25501 aaatttttcc atcacaacca tatcaccttg cttcaacacg ttttccattg aattgccttc
    25561 cactggtacc gggatgaata aaaatccccg caacatgaac gcaaggaata cagaaacaag
    25621 taagtatttg aatacaagcc agaagcgatt aatatagcgt tgtttttttg tcaatctcta
    25681 tcaatccatc cctctttttt tcattctttc tttattataa gaaaaatatc aaaaaaatgc
    25741 gactagatag ctgaaataaa aataattctt tcaattaggc tattggacaa aaaacttttt
    25801 tgatcataaa gtacaaaact atctcttata tgtcatgatt attttctaaa tatttttccc
    25861 atgcttcgtc aaagatatcc atggacggca aatagtcgac attcagttcc agataattag
    25921 aaatttcttc gtagctagag ctgtgcttag gaaattggat gtctttaccg gcttctgtag
    25981 cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa
    26041 agcttcgatt cacccttcgt tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg
    26101 gtttctttta aaattttcag gatgcttttt ataatactct tgatggtctt cttctgctag
    26161 ataaaatggt tgggctggtt cgatttttgt aacgattggc tgttcgaaac gtccactttc
    26221 agctaactgt ttcttacttt tttcagcaat gatccgctgt tcttctgtca aataaaagat
    26281 caccgggcga taattgtcac cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt
    26341 ttgctgccag taaagttcta ctaattgttc atatgaaatc aatgttggat catagataat
    26401 ctccactgct tctgtatgtc cagtcgtttg gctttttact tgttcataag tcggatctgg
    26461 tacatgccct ccagtatatc ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg
    26521 ctggatcata caccagaagc agcctcctgc aaatattgct cgttccatta tgattccccc
    26581 tctaatacta ctcatccgat gattttttat tttcttttgt cttgctttca gtactgctgg
    26641 tgctagtttt gttggtattt gagttgtttg aagcatcaga ggtatctttt ggcaaatata
    26701 gactaaaccg aatgtcatca tttactaagt cgattttatc tgctttaata tacatcccgt
    26761 tttgcattcg gaattgatcc aacctaagca tgatagtcag atcatctgta ttgacttcga
    26821 tccaatctgg taatttatag tttctcttga tcatattcat cacatctttg attggcaaac
    26881 tcaatgttcc gatagaaaga cttctagctt ttagttggac atttccgttt tccatcacat
    26941 acggatcaaa atataaatag aaatcgatag gaaaacctag tacttcgaaa gtaccattga
    27001 gtagtgcttc attttccaaa taaaatttat attcaatatc actatctttt tgatactcac
    27061 taaggaaaaa gctgatgatt tgattgactt tttctttatt tgaattgatc gttaagacag
    27121 gatctccttg tctttccgta agagctggta tttctgggag ttctggttcc cgattagcaa
    27181 aaatacggct gcctaaaaat acggtcgaac caagtactac cccaagaagg ataaggaagg
    27241 cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga tacgtcagat cttgttttca
    27301 tgtttattga tcatcctcct tctctaacca ttggttttgt gtctcgatca gctgatctct
    27361 cactgcattt gccatcaatt gataacctaa attatttgga tggaaatgat cttcttcata
    27421 gagcacatta tttttgatgc catttagatt ttctgtcgtg ctagttgttt gatctgttcc
    27481 ttcttctgtg acacctactt cctgattcaa tccttgataa agcaaatcat tgattgggat
    27541 aaagtagcag ttcggcattt tttttgtcac cgtttctgtt cctttgttcc aattgttaac
    27601 aattgtctgc atgtcagtga tctcaggaaa gttcaaataa aatggattgt agatgcctaa
    27661 aatgtaaata ggtgcggttt tattgaggga tcgaaattct tccaatagtt ctgtgacatt
    27721 ttcctgatag tttttcagtg gctttttaaa ggaattgacc gttaacccga ataaattatt
    27781 ttggataact ttcatcaaat cattaccgcc aacagtcagt gtgataaaat cagctgtttt
    27841 gatattgttt cgaatttctt cttttgtttt tactcgcttt aagatctggt cacttcgttc
    27901 accatttaca ccatagtttt ctgtttcaac cgcggtcaaa ttataacggt cttttagatc
    27961 atttgcgaca aggggaacga atcctccttg cttggtttga tctcctactc cttcagtcag
    28021 cgaatctccg atagctatgt agtgaagttt ttctttttta tttggatctt ctctcacagt
    28081 agttactggt ttcattagtg ggccagcttt cggtatagaa gcagctaaac ctgcatagat
    28141 gatgatcgca attaataatg ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat
    28201 tttttcacct cttgtattcg attggttcta atgttatcaa aaattttatc aaaaaggtag
    28261 tctttatgaa aaaagaaaac aattattttc ctaattttat aaagaaaaag agaataagct
    28321 gcaagccttt caggctgagg gcttatcctc ttttcgacta atctacataa tacatgattg
    28381 caaacgcgtc tttaccagta tgcgtagcga taaccggatt cgtatgcagt acagggatat
    28441 gcatgtccgg gaataattct tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat
    28501 ccgcatgtga aataccgatt tgtcgcacat ttggcaattc tttcagttca tttttcaaat
    28561 tattaaacca tttatcgaac gttttctttc ctcgcccctt agttacaggg attaattcag
    28621 aatgggcgaa attcatcacc acgcgtatgt tcaaaacact agacagcaaa ccagttgttc
    28681 ggctgattct tccacctttt accaagttat ctagtgtgga tattccgatg tataattttg
    28741 tattgtcttg tacttttttg acttcttcaa ggattttctc tactgtttta ccttcttttg
    28801 ctaattcagc tgctcgaatt acctgaaaag ataatccttg gtcagtggta tcactgtcga
    28861 taaccgttac tttcgttttt gttagattac tggcttgtct tgctgcttct actgttccac
    28921 tcaagccttt tgtcatatgg atcgaaatca cttcactgcc gtcttctcct agctcatcat
    28981 acatcgagat gaattctcca attggtggtt ggcttgtttt gggcagattt tttgctttcg
    29041 ccatcatttc cataaatctt tcgccgtcta gttggtcatc atcgggatat actgttccgt
    29101 cgatcatgat ggatagcggc atgacacgaa tgcctaaagc atcgcgtaca tttttttcca
    29161 tcgtacatga agaatctgtt acgattttta catttgtcat tattttctct ctctctttct
    29221 ttaacaagat gccttattca tgttaaacta catgtaagac aatttctatt tttagtataa
    29281 cagacaaata atgatttttc attaattaca cctagtaaag gagccgtttt catggaaaaa
    29341 acgaaattta atcgaacgta tgaaatatta aatgaagtgt ttaatgcagt cacccatgga
    29401 attgggactg gattgagtat cgcgggacta gtggttcttt tagtaaaagg tgcccacttg
    29461 cattcctccc tccatattgt ctcttatgct atctatggat cgatgatgat tcttttattt
    29521 ttaacgtcta ctttgtttca ttcattgatt tttacaaaag caaaaaaggt gttccaagta
    29581 ttcgatcata gttcgatttt tcttttgatt gcaggaagtt atactccttt ttgtttgctg
    29641 agtatccgag gatggctagg ttggacttta tttgtattaa tctggttgtt tgcgattctt
    29701 ggcattatat ataaagcgtt gacgttgcat aaacgggaga gagtatcaaa agtatctacg
    29761 atcatctata tttttatggg gtggctctgt gtcgtggctg ctgttcctct ttatcactct
    29821 cttggaatga ctggtgttat tttgatggta gcaggcgggc tttcttacac gataggcgca
    29881 ttcttttata gcttgaattc tatccgctat atgcatgttg tttggcattt gtttgttatg
    29941 ttaggtgcag gttttatgta cttctctgtt ttattcttta cttaataaaa aaaataaagc
    30001 tatggatggg acagctctaa ataacagcta gtctatccat agctttttta tatcgtcaat
    30061 tcgcctgtcc cgctttttac aggattctca ttacgctgat acgtttcaaa ttgatgcgga
    30121 taacgatttt tttcatccat ttctccttga ctagtattga tcagtgacca atcagaccag
    30181 tcaacaggtg gaaaataagc atctccatcg aatacatgat ggatgactgt acggtataat
    30241 atcgtacaaa atggcagaaa ttcttgataa acagctgacc ctccagcgat gaaaaagata
    30301 ccgtctgttt ctttttccag agcaagcacg tcttccacac tatgcatgac atgcacgcct
    30361 tctgctttat aggaagaatc tctcgtcaaa acaatcgtct gacgattcgg caacggacgg
    30421 ctacccatcc cttcgaatgt ttttcgtccc atcactagtg tatttgcttc tgtcatttgt
    30481 ttgaagaatt tcagatcatt tggcaaacgc caaggaagac ggtcttcttt tccaatcaat
    30541 ccgttttcat cctgtgccca aattgcagct aacattttct catcctttct gattctaaac
    30601 agcgatagga gctttgatcg ccggatgtgg gtcatatcct tctaattgga tatcttccat
    30661 ctcaaaatca aatactgact gcttttcctg attcaaaact aaagtcggaa atgcgcgtat
    30721 ttctcggctc aattgttctt tcatttgttc gatatgattg atataaagat gagcatctcc
    30781 taatgtgtga acaaattcgc caaccgctaa acctgtttca tgtgcaatta ggtgagtcag
    30841 caacgcataa cttgcaatgt tgaacggaac gcctagaaac acatccccac tgcgttgata
    30901 caattggcag cttagccgtc cctcttgaac ataaaattga aacatcgtat gacaaggagg
    30961 caaagccatg ctaggaacat cttccggatt ccatgcagaa acgatcaatc ttctagaatc
    31021 aggagtcttt ttgattgctt cgatcacttg tttgatttga tcgataaagc caccgtcttt
    31081 tgtttcccag tgacgccatt gatagccgta gatgtgtcca agatcgccgt atgtttcagc
    31141 aaaggaatca tcggttagga tcttctcgca aaaaagcttg ctctgctttt tatattcttc
    31201 attgaattca ggatctgtca aacatctatg gccaaaatca gtcatatctg gtccattata
    31261 gtctggactt ttgatgtagc gttcaaatgc ccattcatcc caaatatggt ttttattttg
    31321 taatagatag cggatattcg tatctccttt taaaaaccaa agcaattcgc ttttgatcaa
    31381 tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct ttttgcagat caaaccgcat
    31441 ttggtatccg aataagctta atgtgcctgt gcctgttctg tcttctttac gatgcccttc
    31501 ttccaacaat tttcttccta agtcaagata cgcttgttcc atcctttttc ctcctaatct
    31561 tctacccatt cactcaagta ttcccaccgt gccatttttt cttcaagttg tgcttctgtt
    31621 tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat tatctccttg atgattcatc
    31681 tcttcttgga gcaatgagat tttttcttca agttctgcaa tctcatcttc aatcgtttcc
    31741 cattcttttt gttccatgta agataatttc ttcttttcag caggttcctt gctctgtatt
    31801 tttggtttcg ctttttcagc aggctgctgg gtctcttttt gcttcagtaa ataactggac
    31861 atgtttccgt aataaagttc ttgttttccg tttccttgga atactaataa cttatctgct
    31921 accttgtcca agaaataacg gtcatgggaa acagtgatca cagcacctgg aaaacttcta
    31981 aaataatctt ccaaaatcgt caatgttgct atgtccaaat cattggtcgg ttcatccaat
    32041 aataaaacat taggttgctg aatcagcagt ttcaacaaaa ataaccggcg tttttctccg
    32101 cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt
    32161 aattctgcta ctccgatttg actgccatct gctgtttttg cttcttcagc agcttcttgt
    32221 aagtaagcaa tcatccgttt atctggtgcc atttcttcat tttcttgtgt ataatacgca
    32281 agatgaaccg tttcaccgat agacattgta ccgctatcca ggggaattct gccagctaga
    32341 atattaagca atgtcgattt tcccgcacca tttttaccag taatacctag tctttcccga
    32401 gattgaatca ataaatctaa atgttctagc agtgtttggt catttatacg gtaactgcca
    32461 tctttgattt ccagtacttt tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca
    32521 tcttcttggt ttacttggta caagttctcc tttagatctt cgaaacggtt gatccgtgct
    32581 tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc aagctaattc ctgtttataa
    32641 agccgcttgc gcttttcttc ttgttctact tctacacgat cacgttctgc tttttctaag
    32701 acgtaagctt cataatttcc tttatactcg tataattttc caaatgaaag ttcgaagatt
    32761 cggtttgcta ctctatctag aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg
    32821 tattgtttca aatagttttc caaccactcg atcgcttcat aatctaaatg gtttgttggt
    32881 tcgtctagta atagtaaatc gggttcatca atcaacactt gcgccagact aactcgtttt
    32941 ttctgaccac ctgataaagt actgattttt ttatctaatt cactgattcc taatttttgc
    33001 aaaataatct ttgcgttggt atctgtagtc caagcatctt ctttattcat ttgttcttct
    33061 gctttagcat aacgtttttg cacatcaggg tcttctccgt tttcccctag ttcgatcaat
    33121 gcttcctcat atgctcgaac agtttggatc aaggggctgt ttccttgaaa gactgcttgg
    33181 agaacagtta attcatcgga aaattcagct gcttgagaaa gataaccaat acggtaatca
    33241 gttggataaa acactgtttg ccgatcaccg tcgctgctat cgataccggc aataatattt
    33301 aagagacttg ttttacccgt cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg
    33361 accaaaaaag aaatctggtc aaacaaatct ttttcaccat aagttttttt gaggtcagtt
    33421 acttttaatt ctttcaacat gttccctcct tatcttggat attgtatcat aaaaatagtg
    33481 aacttcagga aaattttttt ggtatttgtt ctttttgtgt tctcaaaaaa agaacaggac
    33541 cgattcgatc ctgttctgat atgttttgtt ttttataaaa cttgaaaatt tttacctttt
    33601 tattttattg gactcgtttt ttttgacttt gctcgaaacg caaatcggaa agaaaagtta
    33661 tcagatggtt tgttcatcgt ataagattct aacaaattga tattcctgcg atagctgttc
    33721 gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat caatgatttt tctttcaaga
    33781 taataacctc ttagcatttt tttcgtaaac agactatctt gctggatcat ccacgaagaa
    33841 atcaaattcc ccgagttcat ttttattcct tcaagtttgc gttgctggat aaaaaattga
    33901 accatcgttt catcataaat atcagtcaag ttatctaaac tttcaataat caactgagta
    33961 tttttttgat atgtctctct gatttcttgg atatcttcat cactaataaa tgtctgacga
    34021 tcagcatatc ttctttgcca aaacattttc ggatggagaa cgattcgcat taacctacgg
    34081 acaacaatga accaaaagcc gataaaagac aaaatctggc ttgtgataga atgttcgaat
    34141 cgagaaagaa aacgggagta aaaacgataa ccattagaag aaagcttgcc cgaacgataa
    34201 ctttcatcca atccatcccg ttcgatcgac aaaatcagtg cttgtatttc ttgtacttct
    34261 tgcttttcat catcggttaa atcttccaag tacagatcac gtatccgttc ttgataattt
    34321 tctatcactg cttcagtggc taaaaattct tttgtattac gatcaatttc ctttatttct
    34381 tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag cattcaaatc cgtgcttttc
    34441 gcttcctctg tttccgtcag cataggcaag acaatgatcc ctatgaccaa agtcactaag
    34501 atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt aaaacatcat attattgatt
    34561 gaaaatggca aaataaaaat cgtagccaaa ctgactgttc cctttacacc gccaaatgtt
    34621 aagatcaaga tctcgttcaa ctgttttttg aactttttac tgccatcttt gaatacataa
    34681 aaaagactga tagaaatgaa tctgatcaca aaaagcatga cagagattaa tacgataatc
    34741 gctaataaaa gaccgtttgg atataaaccg tctccccata ctggcgagaa cacttgcgtc
    34801 aattcgattc ctaaaaatat aaatactaat gcattcagtg taaaagcaat agttgtccat
    34861 gtactgtggg acaagctcga caattctgca tcaaacacag agattttcct aaaaccagaa
    34921 gcttggagaa tacctgctgc cactgcagca atgattccag atacatcaaa tgcctctgcc
    34981 aatacatagg ctagaaaagg aagcaaaagt tcgatcagca gataccctgt gacatcttgt
    35041 gctgatgctt tttcgatcaa atgaattatt ttttgtttga accagacaag caaaaagccg
    35101 atcaaagctc ccccaatact agaaactact agactcatcc ccgcgtttac tgctgaaaaa
    35161 gatccggtga tcagcgcacc aagagcaaac tggaaggcag taacaccaga tgcatcattc
    35221 aaaagacctt ctccttccaa aatatgcatc gcctttttag ggatattcac tcgtccagaa
    35281 agagaactga ctgctaccgc atctgtcgga cctaacgctg ctccaaaagc aaagcaggca
    35341 gcaagtggga cacttggcaa caggaatgat aacgtcgcac ctaccccgac aagggtcaaa
    35401 atgactcctc caaatgccaa gaacaaaatt gtcccgaaat ttttgagaat cgaagaaata
    35461 tctgcttttt ctccttctct aaataacaat ggtgcaataa tcataactaa gaacaattca
    35521 ggttcaaaat cgattgattg tccccactct gtcagaccta ataccactcc aaggaaaatt
    35581 tggatcagtg gagctggaat cattggcaaa atgcggctag ctatgttcga taaggtgatc
    35641 gttactgcaa atacaataac taactcagca aattccatcg tccatcccct ttatttttta
    35701 ttttgtgtaa gcatttttaa aaattgttct tttttcttta aaattttggt ttcgactact
    35761 tcaacctgtg tttggttccc ctctaattca cactgtacac gttttgactc caaacgaagc
    35821 agatcatgtt tcagacgttc caactgacga tacatcaata tatttctttt acgcaactta
    35881 caaagtattt gacggcggtc atccatcgaa agggcgccat agcgatcatg caacagtttt
    35941 ttttgttgtt caaaactctc tctcattttc cagatctcct atacacaaac ttccatcatt
    36001 atagcaaagt tctttttgga tgaaaaatat ttcattgttt catcgtttat tgctagttag
    36061 aaatttccat aaatttttca acgcctcatt ctattccaaa tagtatcaga tatcaagtaa
    36121 aacttaagaa agataaaagt ttctgacaaa aggatgttta ctttcccatt cctgcctttc
    36181 ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa aatcttgtca cagtccctta
    36241 ttctgaataa acggtgtgga agagacagcc tatcagtagt aataagacac atctctttca
    36301 cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat tccccgtata catcttctac
    36361 acgttgatct ggattgatca cgatatacaa gttacctgtt ttttcgctga tttttgttga
    36421 ttgatattga ttgtctaatc cttctgttcc tttatctaga tatgggaagt acacaagatc
    36481 aggtgtgcca tttcggtaaa ggatttccat tcgctcgata tcttcgtatt tcaatgcgcg
    36541 attaaacatg cccatttcca gtccgcctag gttgatatca tgtgtcgcaa ctgcgtcccc
    36601 ttcatggaag atttcgattt taaatccagc acacggatgg atttcaacaa attcactgcc
    36661 attgattcgt ccaaaacttg ttgtgatttg tttgatccaa agatcgccga tatgacggcg
    36721 gttgatcgtc caggtttctc catttctaaa ataaaatttt agcgctgtca tttctttact
    36781 cattaaaaag actcctttat gatgtaattt attcttgtac tacctcagtt tagcacaaat
    36841 ttatgtaagt ggtcacatct tatgtttttt cttctaatga cgtatctgcc cactttaagt
    36901 agccaacaaa gctgagcaga tacagtctag tgttttttaa atcaattttt ctttcgataa
    36961 aaaatccaaa acctgttttt tatcattttt tattctttgt tctacgacgg ctttttcagc
    37021 taattgcaaa gcttcactaa cccacttacc aggttttttt ccagtttctt ctagcaagat
    37081 tttaccatct actgctaatt cgcttaatga cttgataggt aaacgatcat aggattctcc
    37141 tacggcatcc atctgaggtg tttcgtcaaa atagatcatc aattcttcta cttgcagtgc
    37201 atgttttttg cctaagaagt acaattcgaa tggttccaaa gctccagttt ctcgttgtcg
    37261 tagtccttgg acaagtgctt gaacgtcttt aatcatttgg ttagaacact tccacgattt
    37321 caaaaaggaa cgaatctcat tttcttttag atccagttgg ctgatcaata atgcccatgc
    37381 ttggctctct tctttcaatg ggacggatgg taattcagca aaacgtaaaa gtgcttcgcc
    37441 ttcttctttc aaaccgggac agcaaatgta gcactctgtt tcaataaatg catggattcc
    37501 cgcggcccgt tcatttccca agagcatctt gatgaattcc acatttatac gttctacaga
    37561 tattttacct aatagctcgt ggttttcttt gatggcgtag aaggtttctt cctctaattt
    37621 gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt cgtaaggcat cttcatggaa
    37681 ccgctcatga ggattgccta cagctcgcaa gatctgttct tctagatctt gcagaccatc
    37741 gaacaaatcg atgatttctc catcttcttt caaagcaaac gcattgatcg taaaatctct
    37801 tcttttcaaa tcttctgcca acgaacgaac gaattcgaca tgatctggtc tacggtagtc
    37861 ctgataagtt gattccgtcc gaaatgtggt gatctcatat tgttcatcat tttctaatac
    37921 caacacagtt ccatgttcga tcccaatatc tatcgttttt ggaaaaagct gtttgatttc
    37981 ttctggaaat gcgcttgttg cgatatccac atcatggatt ggctggttca gcaagatatc
    38041 acgaacactt ccacctacaa aataagcttc atatcctgca ttctctaatt ttttcagaac
    38101 tggaattgcc ttttggtatt ccagaggtaa ttgttttaat ttcatttttc caacctttcc
    38161 tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg tgactgtttg acaagtccgt
    38221 ttcagagtct tcaaggccag gaccacttca tcttgtttac tcgtccttac gctcaaagcg
    38281 attatggtct ctttgattga atttttccat gtttttttca aaaacttcag ttaagtcgat
    38341 attcaatgaa ttagccatga tcatcgtaac gaataaaaca tcaccaagtt cttctgctac
    38401 cgagttaact ggttcagaag cctttttgct tttttctcca taagtgtggt tgacttctct
    38461 tgccaattca cctacttctt cagttaatcg agccatctga gctaatggag agaaataccc
    38521 caccttgaat tgctggatat attcatccac ttcctgctgc atactgtata aagaacgctt
    38581 tttatccatc tttttccctc caaaactatc ttaccaaaac tttaacgcaa atcctagata
    38641 agagcaattg agaaaatcga atttccatgc tacatttagg aaggaatttc taaatggaaa
    38701 ttctgtgaaa atacataatt gaaagctgga attcatatgg tcaaacgaaa aacagtaaaa
    38761 gaagttcttt ttatcattat aggaacaagt atttatgcat ttggtcttgt ttacttgaac
    38821 atcgctaatc atttagcaga agggggagtc tcagggatca ccttgatctt aagagcttta
    38881 tttggtattg atccagctta tacgacttta ttaatcaaca tcccattgat tttactagga
    38941 ggaaagatcc tcggtaaacg ttccttggcc tatactgttc taggtacact ttctttgtca
    39001 ttctttttat ggatctggca aagggttcca ctagaaatca atttacagca tgatctactg
    39061 atcgtttctc tacttgcggg tttagttgca ggtgtaggaa gcgggatcgt ctaccgcatg
    39121 ggtggaacaa caggcggcag tgatattatc gctcggattt tagaacaaaa ttacggcatc
    39181 actatggggc gttccctatt ggcatttgat atcatcgtct tacttgcttc attaacctat
    39241 atcgacttga aacgtatgat gtatacgttg attgtttcgt atgttttcag tcgtgtgatt
    39301 gattcgatct tagatggcgg atattctgca aaaggtctac ttgtcgtctc caacaaaagc
    39361 gaagagattg caccactact gatgaccggt ctgcaaagag gtgtgacttt cctttctgga
    39421 gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata tggtcgttag ctctagtgaa
    39481 ttaactgaag ctaagaggat cgtccacgag attgacgaaa aagctttcct ttcaatcatc
    39541 aatgtgcatg aagtggaagg gcaaggcttt acttatttga agccacagac aagattactg
    39601 aaaaaactat aagaaaaaag acaaagatag gctatgagga aagattccac atccagctga
    39661 cctttgtctt ttttagtact atactatttt taactaattg ttatgttcac ttacccggtt
    39721 acttttcttt tgtgtctgca aaaggatctt tcggttaact tcatcagata atttcaaaaa
    39781 agcatcccta tctccatctt ctagtgcttg atcgatttga gcataaagca tttttagttt
    39841 tgcctcttct tctttttctt gaaaatatcg ttcaattaat tcagtatctt cttcactgac
    39901 tgaagaatcc caagaagaaa atggattgtc ttctaagata gatagatatt ctcttgtctg
    39961 ccaagcattt tcgaatatac attccacata taaaggcttt ttccaattca aacggatttc
    40021 atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc aaaaacaatt tcatgggttc
    40081 cccttcaaca gatagatcac tgatctgcag tcctcgatcc gcaaaattcg ccccttcaac
    40141 aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga taattcaaga tccaaaaaac
    40201 ttctcttcgg ctaaaagata catgattgac catccacgtt aaaaaattct tcttttcccg
    40261 tacatcaatt agcatttttc tcacctctct gttaggtcat ctaataatga cagtgcctcc
    40321 atatctcctg gttcaagtgt taaataataa gtcaataatt cttgcgctct tttcagctgc
    40381 ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat ctggttcatg tgatagttca
    40441 tgataagcct gttcatagtg tacagctgca acggtaaagt cttctaattc attataagca
    40501 tgggctaggt tccattctgc ataaggatta gcagtttctt ccatctgatt gatcacttgt
    40561 atcacatctt catatctttc atcatccaag taaagattac ttaaagtaag taatgtttca
    40621 tcttgtttgt ctcctacttc taaagctttc agtaaaagtt cttctgctcg ctgtctatca
    40681 tgcagacgga aagcattttc cgaggccagg tgataaagtt cgacttggta aggattttct
    40741 ttgattcctt cttctaacac tgtttgtgct tcttcaatca tttcttcttc ttgaagagct
    40801 tgtcctagat atagatataa cgattgataa tgcggattaa cttctcgaag ttcttccaga
    40861 tagttgattg cctgctgatt ttctttcaat tgaagattcg taaaagctag ctggaacagt
    40921 cgatcatctg tcctttcttc tttcagtgct tcttctaaag gtacgatagc tgcttcgaat
    40981 tctccttgca tacttaagga ttgtcctaaa cgttcctgca tcgagattcc tgaaatttca
    41041 tttatcccgc ttgccaacaa tgtttcataa accactgctg cctctttgaa acgatcaact
    41101 gaaaaataca attcagctag cgcaaactgg atcaatggtt catcaggaag caacccagct
    41161 gcttctttca attttgcttc actcacttca gggattccga taacttggta caaatcagct
    41221 atcgccaata agctttgcgg atagtaatca ctttgcttat ccactttttc caggtattca
    41281 aaagctaagt cgatctcatt atcttcgatt gcaatttctg ctaacgggat gttcagtccg
    41341 tcattttctg ggtattggtt gatcagttgt tcaaaaatct gttttgcttc gatcaaaaag
    41401 ccaatagcca gcaattcttc gcctagttca gctaatatat ctgctgggtc ttcttttaat
    41461 gctttttcta aaaagatttg tgcttgtgac aagtcttcat ctgataaagc ttgcaacatt
    41521 ttttcgctat ttgtattcat ttcaattccc tcttatctca ctgacgattt gttgaaatac
    41581 cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg tatccgtcta ttatacctat
    41641 tttttctaaa acaagtattt ttttgctttt ttctgccaaa aaaagaactt tttccaaata
    41701 ctccatcttt gaaatctgtt ccagccagtg gattggatag ccaagcaagt ccagccacac
    41761 aaaaaagttt tttagttgga aatcaaaagg atatgcttgg agattccaca cgaaatgaaa
    41821 catcattcct aaaaattttt tcatactagc agaaagtaga tggccatttt cggtcagata
    41881 aaaagcttgt tcaaacagtt taccataatt tgacaggttt tctgcctcta attgataaaa
    41941 atagatcatt tcttcgatca tcccggcaaa cggccgagtc gttaattgct ttttatttgg
    42001 atagttttta tacagatttt gcaaaaaacg atgatcacaa atcattccgc aacagataaa
    42061 agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg ctgattgttt gatcataaac
    42121 gatgcgttct ggcagattga tggtttgcag cactacacga tttggtagct gcaaaatcgt
    42181 ccgctgcggt gttaatgctc ggcttagaga acggatcgat acagggatca tccatagtga
    42241 tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga tccatgatcc cttcatttcc
    42301 aaatccgatg ataaacatgg attgattttc gggatagcgt ttgacgaaat caagcaggtt
    42361 ctttaattct gttaaatgat tgcattgagt gttggcacaa atataccagt cattgtttgc
    42421 ttgatttgca aataacgggt tgatcttatc tgcaaacaaa tcataatagc gctgattcgt
    42481 taaaaataag attggctgat caatggaaag ttctcttagc tgactgacta gcgtttctcc
    42541 gtaaaacact ttcgtcaacg catcttctac ttgataatca agttccatac gcatccctcc
    42601 tcttcttatt tttattttac cattagtaag caaattctag aaaagataag agcaaagctc
    42661 acctcataac ttgcaaataa ataaaaatta aataaaatac atgtgttttc tatgtatgac
    42721 atacggattc ggaatctctg tctcttattt attccttttc tgctaggaaa taaaaagaaa
    42781 agcacttgta gccctagagc cacaagcgct ttttcaactg tcttatttaa cagcgtcttt
    42841 taatgcttta cctggtttga atgcaggtac tttgctagct ggaatttcga tttcttcacc
    42901 agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga acttcaaagt taccaaaacc
    42961 gattaattga actttttcgc cgttagctaa agcatcttga atagttgaaa atacagcatc
    43021 tactgctgca gttgcatctt ttttagttaa atcagtagct gaagcaactt tttcgattaa
    43081 ttctgctttg tttgccatgg atagattcac ctcctccaca agagatatga tagaaatatc
    43141 atttagttaa aactttctga gtggtccaga agtttaaaaa gaaatattga ttaacttcaa
    43201 tattctgtta tcacgatatc atatgaaccg cttaatagca aggtttttac caattttcac
    43261 atgctttttt tagtcataga aacgtaacaa acgaaactgt ctttttcttt tgaaaaaaaa
    43321 acgtcgatat ggtaaaatgc ctattttctc ctacgaggga taattcggat ggacgtccct
    43381 tcaaaagtaa atgcttttct gatttgattt tctaagaaac gttcataaga aaaatgcatc
    43441 aattcctctt cattgacaaa aacgacaaat gtaggtggtt tgacagcgac ttgtgtagca
    43501 taaaaaatct tcaggcgttt tcctttatct gttggtgttg gattgattgc tacagcatcc
    43561 ataatgatat cattcaacac agccgaagag atacgaagat tctggttcat acttacttgt
    43621 tcgatcattt caggcagacg ttccaaccgt tgtttagtca ctgcagaaac aaaaacgatt
    43681 ggagcgtaat caagataccg gaattcatca cgaatctctt gttcgaaatc acgcattgta
    43741 ttggtttctt tctttacaag atcccattta ttcacgacga taataattcc acgaccagct
    43801 tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa tcccttcttc agcattcagt
    43861 accatcaaaa cgatatcaga acggtcaatg gcacgcattg cacgcatcac gctgtatttt
    43921 tcagtatttt cgtatacttt tccgcgttta cgcataccag ctgtatctat cattaagaat
    43981 ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt ctcgggtcgt tccttcaata
    44041 tccgaaacaa ttacacgttc ttctcctaaa atcgcattga tcaaagagga tttaccaaca
    44101 tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt cctcttcttc agtctcttca
    44161 gagaaatact tcactgcttc atctaaaata tcaccaatcc ctagtccatg gcttcctgaa
    44221 acaggataag gatcgcctag ccctaaggaa taaaattcat agatatcgtt tctcatttct
    44281 ggattatcta ctttattgac tgctaaaatc actggtttat tacttctgta taaaattttc
    44341 gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc tgactacaca aatgattaca
    44401 tctgcttctt caatcgcgat ttctgcttga tgcttgatct gatccataaa aggttcatcg
    44461 ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc gacctaacca ttcacctttt
    44521 gcataaatac gatcacgagt tactccagga gtatcttcga caatcgagat acgctcacca
    44581 gcgatgcggt taaaaatcgt agacttaccg acgttcgggc ggccaacgat tgcaattgtt
    44641 ggatttgcca tattatttcc tcctcacaag tttccaatct cacttagttt aacgaatgca
    44701 ttgccaactt tcaagcatag attgtcaatt aaacgaagta tttttattcc ttattgaaat
    44761 actaatcgct tttcaaaaga aaaactgaga caaaagcagc tgctttcatc tcagtcacag
    44821 atacaaaatc aattattttt cagaatcatc ggttgctttt tcttctactt cttctttcaa
    44881 cgcatcacct aaaatgtctc ccatagtaaa tccagtattt tcttctggta attcataaga
    44941 ctcaacattt tttggttctt cttctgaagc tggtttttct tctaaagctt tgatactcaa
    45001 tgcaatacga tgttcttctg gatggatctc taatactttc actttcactt cgtcaccttc
    45061 atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa atttgagaga tatggactag
    45121 cccttctact ccagggaaca cttcaacaaa ggcaccgaag cttgtcaaac gtttaactgt
    45181 tccgtctaat actgtgccaa ctggagcttt tgtatcgatt tctgaccaag gtccaggctg
    45241 tgtgtctttg attgacaagg aaacacgttc ttgttcagga ttgatagaca acactttgac
    45301 atttacatca tcattgacag ccaatacatc acttggtttt gctacatgac tatgtgaaat
    45361 ctctgagaca tggactagac cgtcaattcc gcctaggtcg acaaatgcgc caaaatcagt
    45421 aagacgtgca acacgtccac tgagaacatc tccctcatgg atagttgaaa gaatttcttt
    45481 tttcttttct tctctttcgt tttccactac cgctttatgc gataagatca aacgattttc
    45541 tgatggttcg atttcgatga ttttaaagct caatgttttg cctttatagt ctgaaaagtc
    45601 tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa ccacgtacac cgacgtcaac
    45661 gactaaaccg cctttgacaa catttgtaac aggtgcttcg atgaccttgc cctcttggaa
    45721 atctctttcg atatcttccc aaactttttt agcatctaaa cggcgttttg aaagtagata
    45781 gctgccgttt tctttatctt tgccgatcgt actgataacg actaaatcta atacatcacc
    45841 gacttttact acttcgctga tatcttctac cggcaaagtt gataattctt ttgccggaac
    45901 gactccttca actccagccc cttcgattcc gacaatcact tgtttgtctt caatgactaa
    45961 aacttcgccc ttaacaacat cgccaacttt tacttcttgt acgcttttca ttgcgtcttc
    46021 cattgactgg ttgttctctt gattttgatc aaattctgtc ataacacttg tcctcctaac
    46081 caacattctg ccgtaagtac ggtactgttt ttagtttaat tgattattca aaaaaaataa
    46141 agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt catttttttt cttaatttaa
    46201 aaaagaagat aacctttttt caaaacaaca gcttttattg ctgcaacgac ttctggaatc
    46261 gatttgccag tcgtatcgat acgaacagca tcctctgctt gcttcagcgg agagacgtcc
    46321 cttgttgaat cataatggtc tctttcctgt atctctttcg tcagtgtttc aagatcagtc
    46381 ggaattcctt tttcttggtt ctctttgaaa cgtctttctg cacgctcact tacgcttgct
    46441 actaaaaaga ttttcacttc tgcattcggt aatacagctg tcccgatgtc tcttccgtcc
    46501 atgacaacgc caccgcgttc ccctattttt cgttggacag caactaattc ttctctgact
    46561 tttccatgtg cagagacttc agaaacagct tttgtgacat ctggttgacg gatttctaaa
    46621 gtcacatctt ttgatgctat gaatacatgc tgtccatctt cttgttgcga aaaagagata
    46681 ggatactttt tgatcagcga aactagccct tcttcatctg aaaaagatac attgttcgta
    46741 atagctaaat aagttactgc gcgatacatg gcaccggtat ctgtataaat ataattcaat
    46801 tcttttgcta atatcttggc aacggtactt ttcccagatg aagcaggccc gtcgattgca
    46861 atactgattt gtgtcatgat ataactcctt tttctcagaa aaaaagccgc aaagcgcggc
    46921 tattattatt taattcttaa ttcttgacca gggtaaagca taaagttgtt tggatctaag
    46981 ccattcaatt caaacaattg atcagtggtg atccctgcac gctcagccac ttgttttgga
    47041 ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt
    47101 tctgctgcat tgctgcttga atcccctgct tgataagttg cctcacttga ggaagtcggt
    47161 gttgtctctg ctgcaatact gctttctgac gttacacctg tatcttgaga cacagtagat
    47221 tcttccgcga ttgtactaga ttctactgtt gaagtctcag ttgactcctc ttttgttgta
    47281 cttgtagaac tttctactac tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg
    47341 ttgcttgatc catttctgat ccagaaataa gcacctgctg gaatagcgat acataagacc
    47401 agtaaaatca aaaatagcga aagaaataat gtatttcctt tcttttgttg tctctgagaa
    47461 cttcttgatg aaacttcatc atctgtatcg tagattggtt gttcccatgg ttcttgtgtt
    47521 tcgttatttt ctgtagattg atgtctatct tttctgctca ctgttttacc tcctaaactc
    47581 tatcgtgtgt attataccat aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt
    47641 tttttttaaa taaatttagt aaaatcgttc cccaatgaag ttgttcagtc ttagaaaaaa
    47701 gcttcctctt tggcagaagt tctgcttttg ctccatcaat atcacaacaa ttctgcggct
    47761 tttcggagag tttttcgcca aaataagcta agatgaattt tctgcggcag ttttcttcat
    47821 taatatagcg aagcatctgc tgcaggcgga attctttttc tttttcctga cgtttgagcc
    47881 gttccaccca attttctggt ttttcagact gctggatcag ctccatccat tttttttgca
    47941 gctcatcaaa gggagcttgc tctgtggcat attccaaata aagctcaaaa ctttgtcgct
    48001 gttctctaga aagttgatta aagaaataat ggatacgttc atccccactt tgataaaaaa
    48061 gaattgctgc actttcttcc tgatctctgc ctgctcgacc gatttcttgt acatagtttt
    48121 ctagactgtc tggcaaatca taatggacca cataacggat atcagattta tcgatcccca
    48181 taccaaatgc attcgtagca atgagaaact gcagctgatt ttttacaaac tgctgttgaa
    48241 gttgtcttct ttgagctgca tcaagaccac cgtgatagta tccaactgta aaacgttccc
    48301 tgaataaatg atacaatcgt tcgacttctt tttttgttgc acaataaatg attgccgctc
    48361 cttgtgctct ttccatgaag tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa
    48421 aaagcgagat attttgccga ttgactgatt gtctgatctc ttttggtggt cgatcaaata
    48481 gtacatgacg tatatctctt gctactaaat ctgttgctgt cgccgttagt gccagtgtga
    48541 ccgggtttcc taaaagcttt cggatttttc ctaactgttg atattctgga cggaaatcaa
    48601 ctccccattg agacacgcaa tgagcctcat ctactacata taacgcaata ttttgttttt
    48661 tcagatgctc taaaagagaa ggattagtca acatttcggg actcaaaaac aaaaatttat
    48721 attgcgatag atgacggagg acgtatcttc tttcagattg cgaaagtaaa ctattgaaag
    48781 cagctactcg tttctcccct cgtttttgta attgcgtcac ttgatcttcc attagagaaa
    48841 gcagcggtga cacgatgatg accagacctt ccattaaata ccccgtcaat tgatagcaaa
    48901 ggctttttcc cgttcctgta ggcaaaatag cgagagtatc ttctccatca agcaacgcct
    48961 gaataatttc ttcttggccg ggacgaaatg aatcaaatcc aaaatatttt tttaattcat
    49021 ttattaacat tctgtatcgc ttcctttctc aatagataga tttgatacaa acgaaattct
    49081 ccataatcta attgtccatc tacattccat tcgctaaagc gccactctcg cacggaatcc
    49141 ttatttggtt ccaaacgttc gattgtttct gcagagagaa tccactcgta tggaaaatca
    49201 tcaaaaaaca aggcccactc aataagatga tctgttactg ttcctctttt taaatgacgg
    49261 attgccatga tctggtcaat tgtctgacca ttcataaaca tctgcttggt gatcatcatg
    49321 ctttgattga aattttgctg taataatgaa tcgatcaaag cgtgccaaag cgaatctggc
    49381 cgtttttcga tttgtgctaa caaaaggtgg atacatctgc tttgttgcag ataaaccgct
    49441 gactcatcat tgttttttgc cagctgataa ggaagcagcc ctgttcgctc atttccagat
    49501 aattggttag ctaaaaaatc tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa
    49561 ctctcatacg cagtatcaat caaaatttca cgtggcaatt ggctttcact aagccatttt
    49621 tttagttgga aagtataaaa aggacttgtc tctgctggaa gatagtcttg aatacccgtt
    49681 gccagattag aaattacttg aacagcaaat ttaatgagcc gccaactagt ttcccctgtt
    49741 ctgccatagc ggtcaaaacg caaaccagta tgttctattt gatgttcgga caacatatca
    49801 gcccctgcag gcgttagctt tgcttcattt tcatttattt cgatccaacc ttgctgcgca
    49861 attttttgca aaatttgata aaacttatcc tgcttcaatt ctggaaaact gttatggata
    49921 aacaaaagtt cgtgggtaaa tccataaata agtactgaag tcgttcgttt tcccacaagg
    49981 agttggtaaa gagaactaat ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa
    50041 acaatcatat tattctcctt aagttaggat ggttagaaat gtcactacta tgtaaacttg
    50101 tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga agcgccagat attttcgatt
    50161 atgatgaaga aggaatcgtt ttatttgccc aagaaccaga ggctcatcaa cagttcgtga
    50221 tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt
    50281 tattagaaaa taaaatataa atataaacga aactttaaaa acgtacacta agttctctaa
    50341 acttgaaaat aagccgggat ctgtttctct tgttccgtga aatcccgact tattttgaaa
    50401 gattcatttg ctatctatag ttaattgact agttttttca cggaccctat aggacccatt
    50461 tatattattt caactttttc agccagtttt ccaatgtatt gacgatcaca gtttgctggt
    50521 ctgcgttaga aatttctgcc tctccatctc ctttttgctc accatagctg ccaaaacctc
    50581 catgatttcc gccattgatt gatacatact ctgttctttc cggtaaatac tgcttagctt
    50641 cttgataagc ttcctgattc aatactttat cttttgtcgc cgtgatcgat aaaacaggaa
    50701 cagctgattc tgtcaatgtt ccttttttgt cgggataact tgccatgaag aatacccctt
    50761 ttaaatgttt gtctgaatga ttcttattga cataacgact tgccattacg ccacctaatg
    50821 aatggccacc gatcacataa tcggaatttg gaaaatcttc tttgacctta tccgctttgt
    50881 ttccgcccat cactgctaga tcaagtggca ttttgacaat agcgactgaa aagccgtttt
    50941 ttgctaaatc ttgcgcccaa atactataac tttctggctc tacgagagca ccaggataaa
    51001 aaataagtaa tggagacttt ccatgattct ttttctctcc ttcaaaaaat aagtaatctt
    51061 ctttgttttt tccttctttt accaacactt gcgcttctga cgatggttcg tacgtatttt
    51121 gctgtatata gtaataaccg gctccaccga gtaatcctaa aatgatgact aagccaatta
    51181 gtattcgtct aaatattttc actttctccc accttttcta tctgagtgac ccctttttct
    51241 taatcaagta tgattatacc ttaaaaaaat aagagagaaa tgcaaacttt ccagcatcaa
    51301 ataaaaaaag acggaatatc catttgaata tccgcccttt ttgtatgttt atttttattt
    51361 agcacataaa cgatacacag cttctgcata aatttccatt gctttataca tactacttaa
    51421 ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa
    51481 tgccacacac tggttcatcg tacgagcaaa agtagcgccg cctgatacaa acggttcagt
    51541 catgtctcca gtaatatcgc gatatgtccc caatagattt ttcactaact cactgtctag
    51601 tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa gtcaacccat atttttctgc
    51661 tgttttagtt aatttagcta ctaaatcatc tttcttgaat gtaacaggga ttctcatatc
    51721 tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt gcaaagttca tcgttagctc
    51781 gccggattgt tcatcttctg ttttgcccac gacagcttca ccagttgcat tttcttgaac
    51841 taattttcct aagaaattga ttgggccaaa atcaaatact tctgaaagag caattgccag
    51901 acgagaaata gcattcactc cttgtgctgc atctttcgcg tggatacttt taccaagaac
    51961 gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt tttttcaatg cttcttttac
    52021 ttcagaaagc ttttctccag agtaaggagc tgcatcagga acaacattca acgctcctcc
    52081 agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag taagcttgca ataagccttt
    52141 ttctgcataa attaaaggaa attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc
    52201 tttttcgtta tatttttcta aacaacgcca taaattttct tcatctgtac caaagataaa
    52261 acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa gctttgacgg catacattgc
    52321 cgcaatagaa ggacctttgt catcttggga tccgcgacca actaaccagc cgtcttttat
    52381 agttggatcg aatggttttg tttcccaatt attttcgtcc cctgctggaa caacgtccat
    52441 atgacacaag ataccgaata attcatctcc agagccaatc tctgaataac cgtaatagcc
    52501 ttctggatct ttaaatgttc taaatcctag cttttcactg atttccaata ctttatccaa
    52561 agcttcaatc acttttttac cgaagggatg tccttgtcct gaatctgctt catctagtac
    52621 ggaaggaatt cggatcaatt cgctcaatga ttctaatgct tgttcatgat gttgttctgt
    52681 cacaaatttt ttcatgatat tatcctccta atatgttaaa aagggtcatg tcaaattcct
    52741 ccgacacaac ctcttcttct caaataatga cgttttaagc ctgctccatc agcaagcaga
    52801 atgttcttaa caaatgatga aagaaaaaac actcactgaa tggagctatt gacttatcca
    52861 cgtgcttatc gatagccgtt tatcggtttc ttcattctta tgatagacct tgtacctgta
    52921 tttgtctagg tttagaacat tgctgctaca cctaggatca cacagatggc tacaaataat
    52981 atcgcaatta atttaccggt aaatttccac cacactgaaa catcgaaacg agcaattgct
    53041 acagcaccca ttacgatagc agatgttggt gtaattaagt ttactaaacc agatgcagat
    53101 tgataagcag tgattactaa atccttaccg acgccggcaa attcacccat aggtcccatg
    53161 attcccattg ttgcagcagc taagccggaa gttgaaggaa tcaagaatga catagggata
    53221 tagaaaataa atgtaataat gatgaataca cttgaagata atgaacttaa tccttgttcg
    53281 ccccaatgta agatggtgtc tgtgattaat ccattgttca taaccacttg gataccacga
    53341 gcaacagcta ctacgattgc tacaccgatc aaatcagaag caccagctaa aaattcagag
    53401 acaaattcag actctttcat gccataagtg attgctacta cgatggacat tactaagaat
    53461 aacatagtaa tttctgggaa gtaccagtca cctaatggaa gcatgctttt acctagaacc
    53521 gtacctagta ccggaaggtt tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc
    53581 gcattcaagg ttgtccaagg aatcaaactg atgatcatga taccaaaagt aataaagaac
    53641 aatacattaa catgtttttg ttttttcgtg attacttctt gacgaccgat tgcttcaata
    53701 tcgaaatgct tcatatcttc tttacgttgg ttgtatacta atgattttgt tggatctttt
    53761 tcgatctttg atgcataacg atacacatat acaatagaaa tagctaacaa aataacgaac
    53821 atcaagaatc gaagaccaat accttcaccc atcgaaatcc caacagcttg tgaagctacg
    53881 cctgttgcaa aaggatttac cgttgaagct agacagccta cttgagaccc tactaatacg
    53941 attgctacag ctactactgt atcaaagcct acagccatca tgactgggat cagtaaagca
    54001 tagaatggaa tcgtttcttc tgccattccg taagtagagc cgcctaatgc aaataacagc
    54061 atcaatactg gaataagcat tttttctttt cctttgaatt tcttcacaac tgaagcaatc
    54121 ccggcatcta gtgcccctgt tttgttgata atacctaaaa atccaccaac aaccaaaatg
    54181 aagaaagaga ttgggattgc agcaggtgtt tctttagtta gctcccccgc tggtacacct
    54241 agcatcccat tgattggcgc catgaaaata tcccacagcc cttgaggatt ttgttgtact
    54301 cgttcatatg atccagcgac gatgttgcct gcatcgtcta cactatagtg cccagctggt
    54361 ataaaccaag taagtacagc gattactgca ataataataa ataagaccgt atacgctgaa
    54421 ggcatttgaa attttttctt ttttgtttgt tccatcttct tcttcctttc ttttccgtta
    54481 gagacaacaa ttttggctct aatgttattt tatcaaaaag agtatcagaa caaaaataat
    54541 aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt ttccctattc cctcctcttt
    54601 tttcaggttt ttaaagcttt cttctttttc attgtagcgt ttcctttttt ttataaacaa
    54661 acattttcgt cttttaaaag gagaagaatt tcacatttgc aaagaattga ttgattttta
    54721 tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac gtttacatta ttgcatgtct
    54781 tgtatttttt tccaaaaaaa aagaccaaga aatttcttga tctttttgat atttatttga
    54841 ctgctgtatg acgttgttcg aactgatgtt gttttcgact taaccatgga agaagttgcg
    54901 catgcagcac taaaaagaca gcactcacga tgaatccttt gatcaagttg aaaggaacga
    54961 tcccaatcag tacatagttt gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta
    55021 aaaacattaa gtaaagaggc gtaataacga aataatttgc gatactcata aaaatagtta
    55081 gagcgatggt tcctgtaatg acacctaaaa atttgtttct tcctttatgc tgtttcttta
    55141 cactaaagaa ataataaatc gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt
    55201 ctcctaccaa attatccgga gaaaaacctg tagtcagcag atgtaatact gaacgcaaga
    55261 acgctgttgc aactccggcc gctggaccga ataagaacat gctgatcaat accgagatat
    55321 cactaaaatc gatcttcaaa aaactaaaag ccggcatgat cggaaaagca aaatactgta
    55381 aaactaatgc gatcgcagca aacatcgcta ctgcaaccat tttctgtaca cgggtgttct
    55441 tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac tttttgacgt ttctctccaa
    55501 atgaaaaaac tctattttcc agggaaaata gagttagagt tatttgatca agcgtcgtca
    55561 aataagctct atcttcttta tccagactat actgtcggta tcggaatttc accgattcag
    55621 ctattttttt gcaaaatagg tcgtggacta taaccaccgg tcgggaattt caccctgccc
    55681 ctgaagacga accttatata atttttgttt acatattcat tatacatatc tattcaaaaa
    55741 acgcaacatc taacacttat aaaaagtaag tgttcatttt gagtggttca gcaaactgct
    55801 gacttctttt ttgttcagtt cacggtaatc cccaggacgt aggccttgta aagtcagatc
    55861 tccgtatttt tcacgtttta gtttttgcac aggaagacca actgcttgaa gcatattttt
    55921 cacttgatga ttacgtcctt cgtggatcgt caattccacg atactgtggt ttgtcttagg
    55981 atccactgat aaaatttgga atttagctgg agatgttttt cgtccttcga tctttattcc
    56041 ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct tttacctttg caacgtaaac
    56101 tttatccact tcatgttttg gatgtgtcag gcgctgagca aaatctccat catttgtcag
    56161 tagcaagatc cccgaagtat cgtaatccag tcgacctaca ggatagatac gttcttttac
    56221 catcggcaaa agatccgtaa caacttttct tcctttgtca tctgcaacag cagaaataac
    56281 tcctttaggt ttgtacaata aatagtaacc gtattcttct tgataaattg ggacttcatc
    56341 cacttccaca gtgtcatgtt tcccaacttt tatccctaat tcccttacaa cttgtccgtt
    56401 tacttttaca cgtcctgcaa ggatgaattc ttctgctttt cgacgagaag tgatgccagc
    56461 gtgggcaatt actttctgca atctctccat tacaattatt ctcctttatt cattccatca
    56521 aagaatagat ctggtgacac gtcttcttca agtgaaattt ccatttcttg tgtatcaggc
    56581 agttcttcta atgatttcaa accaaaataa tctaagaagt agccagttgt tccatataat
    56641 atcggccgac ctggtccttc tactctacct ttttcttcaa tcagtttatg ggcagctaga
    56701 cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct cgatttcagc tcttgtgatt
    56761 ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat
    56821 gtcccttgcg catatcgctt aagtaaggga gccagctctt ttttagtcgt taatacataa
    56881 tggctttccg tttcaaaaat atgcaaagct gagtgcgagt tttgagtgta agattctttt
    56941 aattctttca ctaaacttgt gacttctgct tcgctgagat ttaacagtcc gcttaattct
    57001 tcattactga tcccttcctc gccaacaata aaaagaaggg cttcaagttc acttaatttc
    57061 gtcatgttcg gtatctccta ctgctggata aagcataatt gattcatagt tttctttttg
    57121 ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa gccataaaag tagtgacaac
    57181 ttcactttta gaaggtgact caaaaaaaga atcgaaagta cagccatgcg catttctttt
    57241 tatacgctct tcgataaata ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt
    57301 tttttccact ttttgagaaa atttcttacg ttctaagatt ttctgcatgg cacggatcaa
    57361 atcgattttt ttgacttgtc ctcgcttcaa cggcttttca gcatccgcta acccatcgat
    57421 gttcataggt tcttttgtgt aataaagact tcgttcctct gctttttctg tcagtctttc
    57481 agctgcatat ttatatttac ggtattctaa aagctgagtg accaattctt ctcgaggatc
    57541 ttcttcccat aattcttcat cgtctgtgac gaattcttgg acaggaagca atgtcttgct
    57601 tttgattgcc atcaatgtag cagccatcac taagtattct cctgcaacag ctagctcgag
    57661 tgttttcatt gtatggatat aattcatata ttgctccgtg atcgtagcga tcggtatatc
    57721 atatatatcg atttccattt gctgaatcag atgaagcagc agatcgagag gaccctcgaa
    57781 tacatctaat ttaatattga tctccgtcaa ctcattcact ctttcttctt tttctttgct
    57841 agccattttc caatgtaagg ctctgtgatt tcagttgcac ggatggagac atccgggtac
    57901 ttttcctgta attcatctaa catttgaaag cccatattct caccgcgata agacggattc
    57961 aaagaaatgt catggatgat cagctgatcg ttcgttgccc agtaaatacc aataatccct
    58021 tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg
    58081 tacgtatcaa tctccttcaa caaagaagtt ttacctgtca gtttcttatg gaaactcaat
    58141 agtcccatag caattttacg gttttctttc cggtaatgtg ttaacatctt ctcaccttcc
    58201 tatgactaag cccttggaaa atgctgctta taaacatccg tcattctttt cttcgtgata
    58261 tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta acaattcctg tactgtccgc
    58321 aaatcagctc cattttctag cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt
    58381 acttctttgt aaatcccagc ctctcgtact aactgcttca gatttttcca gattccttgc
    58441 cgagaaagac ctttcccatg attattgaca aatacatgca tttcagatgc gtcctttaca
    58501 agtaatggac gtgcttcgtc taagtagcgt tccagccatt ggatcgcata atcacctaaa
    58561 ggaatgatcc gctctttatc tccttttcca actgtctgta ccagtccaag agaaagatga
    58621 agatctccca atttcaatcc aatcaattca ctgacgcgca tcccagtagc atacatgact
    58681 tccaagattg cacgatctct tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc
    58741 ttttctactt ctgtcagagt caatgtactt ggtaatttct gtgctttctt cggcgtatcg
    58801 atatgttgca tcggatcatg atcagtgata cgctcttgcc gtaaaaactg atggaatctt
    58861 ctgagacttg agatcatccg agtaacagtt gctgtcgctt ttttttcttc atgcaacttc
    58921 tccagaaaac ggatgactaa aaagcgatcg acatcctgcc atgaagcaat tttctgctcc
    58981 tgtagaaaat gcaagtactg ttccaaatca cgttcatagc tttgtcgtgt attggcggac
    59041 aatccccgtt caatattcaa gtagtggaga taatcgttga tttgctcttt cataataatc
    59101 cccctcatgc ctctctatca tatcagatac cgcttcattc tgatagtctt tttccataat
    59161 ctgagcaaag aaaaagccgt ctagacactc agacggactg gtttcttttt tctttttcat
    59221 agcattcctg acagattcca tggaacgtta gccgatgatc ttttactaaa aaatgataac
    59281 gttcttctac tacttgctcc acttcaagta aaagatcctc ttctacttcc tcgatcgtgc
    59341 cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc gccttctttt ctcaaatcat
    59401 aacgggctaa tccatcatta aaacttactt tatcgacaat tttcaattct gtcaagattt
    59461 caagggttcg ataaacagta gcaagtccaa tttccggact cttttttttc actaaaaaga
    59521 agatttcttc tgcagataaa tgctctttct cattttccaa aagcacaagc agagtagctt
    59581 ctctttgcgg tgtcagctta aaccccgctt catgtagctg ctgttttgtt tttttcatcg
    59641 ccgaaatagc attcatgacc atttactcaa ccccaacaat ttataataat tacaaataaa
    59701 aaaagatata acactatgtt aattaaaatt attataagct agttaagaaa aacatgcaag
    59761 ggattctcaa ttagattttt tgactagttt tgtcacaccg ttttcttgtg taatcaatcc
    59821 ttgtttcaaa agattaccta atgcgcgctt aaatgctcct ttgctgatac caaaagcttt
    59881 catgatttct tctggatccg atttatctgt aaagttgatt tgctgatctg cagaacgttc
    59941 taaaatggtt aaaatcattt gtgcatcatc tgatatagct tcatatgctc ttggcttcaa
    60001 tgaaagattc agtacaccgt ctggtcggac accaatcact cgacctttta tctgctcgcc
    60061 tagacgaggt tcattatatc gttcagaagg atggataaac ccaagataga aatcttccgt
    60121 taaaacgtac gtacctgtta atttcaagcg gtatgctgtt cctatcacat ctttgttttt
    60181 catttcttct gttccatatt tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc
    60241 ccaaatacgg tcttttgcat caactctaag ggaaatcatc aagcgatccc cttttttagg
    60301 ccatagctct gtcattgtcg gcaattcatc aagagatacg acaaagtctt tatctggaag
    60361 accgatatcg acaaacgcac ctaaatcttt tcgtgtagca gtaacgattc caaaagcgta
    60421 atgaccgata cggcttttag gaatttgtgt agtgatccga ttttcatgtt tttgattttg
    60481 gtaaccgaat ccttcaactg cttcaccgat tttgtgttca ccttctgatt tgcttaagcc
    60541 aaaggtttgt ccgttttttt ggacaaagta gtttttttca ttttcatcaa taataatacc
    60601 tgtaaatata gttgctaata aatcgttcat ttttttctcc ctctatagtg aaaggttttc
    60661 ttaatacatt tctttgctat cataacataa tgttatctac ttgaatagct gagccttggt
    60721 ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca tactcccctt tttcgaataa
    60781 acggtgttaa acggcaggct cacagcctct ttcatttgcc taaaattaat tgctcccatt
    60841 tcctccgaac catccagttt tacagttgat ggttacgatg taaaatggca aagcactttc
    60901 tccaatgcct ttcatcaatg cagattttgt tgaagtggca tatagattat aatagccttc
    60961 cccattttcg attctggctt tttctaatct gtaacctcct tcaacgatat atccacgttg
    61021 gatacatgta gcaatcactt tgctttggac acctggtgcc cacgtccatg ctggattaga
    61081 tgcatcggca atatcacttg ctctggcaga tttgattggc tgattcgctg agtttgaaga
    61141 ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc
    61201 ggcttgagct gcagctgctg cttgagcggc ttgttttgct tgttcttcag ctgctttttt
    61261 cgcttcttct gcttttttct tttcctcttc ttcttttttc tctttatcag tgagcatctt
    61321 atctacttgt ttcaattgtt cattcaactg agcagataga tctgtatttt tcactttttc
    61381 tactgcttct tttgccgtct tgtattggtc gcgtgttgct gaatccacta cttttccgtc
    61441 tttaaagact atagctgttt tttctttagc tgtttgcaat tcagcatatt gatttttagc
    61501 ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg ttatccgttt cgattgcttg
    61561 gatctcttta tctgctttca acttgatgtc atctgctaga tgatcatcga cgatggcagg
    61621 tgctatgaac cactcattca ctttttggat atctcggatc ttagattgga tatcttggta
    61681 agttgtttct aactctgaat atcctttttc attcttaaga ctggataaat cattttttag
    61741 ttttgataaa tcttgatgaa tcatatctgc acgaataaat tgatggtgat catctgtata
    61801 aaaggcagca agtttctcat gaagttcttc catttgttgc tgtctttttg cttctgctgc
    61861 tacttcggct tctacttttt gatgatggtc ataataagcc cagcttcctc caccgagtag
    61921 aagtactgct gctaaaacga gataaggtac tttttttgat tttttctttt gcgctttatc
    61981 tggatctgtt ttttctaagt ttgattcttg atgtgttttt tcagtatctg gcacgattgt
    62041 tattgatttc gcttctgctt caaacagctt aggctggttc tcttcttttt ctatttttgc
    62101 ttcaagatca gtaccctctt tgcttacaga ttgattttca gcatcggatt cactatttat
    62161 ttcatcgggc tctgttgact ttttgtctgt gttttcgagt atttcttttt tctctgcttc
    62221 cggcagttta ttcgtttttt ccttttctaa ttgtttttca gtaaatactt cagttttttc
    62281 tgtgatatcc gtttttacgt cttttatttt tactgtttct ttacctgtgg cgttttctct
    62341 atcagagtct ttttcagttt cttgttcctt ttctggctct actttgtttt cctgtgcaat
    62401 tttttcactg atcattttga gcagctcttt cgcgtgctct tcttttgtat cttcacgatg
    62461 tgctttgata taagcggata gtatcgaatt ttcttcctca ttatcgacga tttctttcag
    62521 ttcagctact cttctttttt gttttgattc aagcgtttct tcttgattct ttcctgattc
    62581 ttccgaagtc tcatgaacag ttgctttctt ctgttcattt ttctctatat tagttacttg
    62641 atcgtgagca ggtggttctt cgtcttctgg ttctgctttt tcgaataatt ccattacaga
    62701 ttctagaggt aaatctttca attccgacca ttcgattgga tcgtttaatt ctgtatcagg
    62761 aaagactgca tcttcagttg atttatcctg catagctgca tctgtatttg tttcttcaat
    62821 tgattctttt atttgctgat cttctgcagt tatttgatga ccgcagtctg gacagatagt
    62881 attctgctcc atttttttcg atccgcaatt tggacatttt ttcatcactt agcgatcggc
    62941 tcctttacta ggtttacgct accatttaat ctatcatatt ctatacgtca cgccaatagc
    63001 ttatttttag gatttactta aaaataatga atgaatttcc ttcctataga aaaaaaagct
    63061 tgagactgcg tagtctcaag ctttttttct tttaaaataa accaaacagt tttactgatt
    63121 tctcttctgg tcaatagtcc acgcttattc taagaaatct tttaattgtt ttgaacgaga
    63181 aggatggcgc aattttctta atgctttagc ctcgatctga cgaatacgtt cccgagtaac
    63241 accaaatact ttccctactt cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg
    63301 taagcgaagg acattctctt cccgatcagt caatgtatct agtacatctt ccaattgttc
    63361 ttttagtaac tcataagctg cgtgttctgc tggacttgtt gcttcctggt cttctatgaa
    63421 atcacctaag tgagagtcat cttcttcacc gattggcgtt tcaagggaaa caggttcttg
    63481 cgcgattttc aagatttctc ggactttttc tgttggaaga tccatttccg caccgatttc
    63541 ttctggcgta ggttctcttc caaggtcttg cagcaattga cgttggatac gtatcaattt
    63601 attaattgtt tcaaccatat gaactggaat acggatcgta cgtgcttggt cagcgattgc
    63661 tcgagtgatg gcttgacgaa tccaccatgt agcataggtt gaaaacttaa atcctttgcg
    63721 gtagtcaaat ttttcaactg ccttcatcag tcccatgttt ccttcttgga tcaagtcaag
    63781 gaactgcatg cctcgtccta cataacgttt agcgatactt actactaaac gaaggtttgc
    63841 ttctgccaga cgctgtttcg cttcttggtc gccttcttcg attttcaatg ctaacgctac
    63901 ttcttcttcc gcagttaaaa gggagacacg gccaatttct ttcagataca tacgaaccgg
    63961 gtcattgatt tttacaccgg ttggcgcaga aagatcttct gtttttgctt gttcagcttt
    64021 tttctcagca ctcttcaagc tgtgaattga tggatcacca ttttcatcca ccacactgat
    64081 acctgcgtct tcaatctttt ggatcaattt gtccatttca tctgcattca aagtgaatgg
    64141 tgtagcaagc tgattggata aatcatcata tacaacttgt cgtttgggtt tgttttcttt
    64201 gatgaaagct gctactgctg cttcatacgt ttttttatct gttccatttg ccataaaaga
    64261 aggcccccct tgagtagcta tttggcttgt tttaactgtt tcgttaaatt gatgatttca
    64321 atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt
    64381 ttttttaatg ttatttcatc agctaagcca gatttgctga tcacatctag caagtcttgg
    64441 atttctcgtg gcgaactttc ttctgccacc ggtaaatatt cgatttccac caccaaccgc
    64501 cttaaatttt catcttgcaa aaagtctaaa aactctgcaa ggttgaattc ataatgttgt
    64561 gagacatatg catcgaataa aaaataaact tctgcatatg catcatgaat aaactgaaat
    64621 tctcgttctt ttaaaatatt acggacagaa acttctttaa atgcacgata taatagaatc
    64681 tgctctgcct tttctacttg cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta
    64741 acctgagtgt gttgactttc ctgaggttct tgacgatttt gctgatttgt ccttcgaaga
    64801 tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa actctgttga cagttgagat
    64861 agatacatat cttgttcgat tggtgaaaca actcggctaa gttcaactag taattcttcg
    64921 aggtattcga tcttgtcttt ttcattatct aaatttttcc cttgttttaa ataacgtgat
    64981 ttaaaagtaa agactgtttc gcgcccatga gccactaatt cttggaaaga tgcttctcca
    65041 tacttacgga gatattcatc aggatcaagc ttttctggaa cgactacgac agaaagtgct
    65101 aatcgactgt tttgccgaag caattcaatc cctcgattgg ttgcttcaaa acctgcgttg
    65161 tctccgtcat agcaaaagac caattctttc gtcagctttt ccatttgttg gatctgttga
    65221 gctgttagac ttgtccccat agaagctaat ccagttttga taccagcctg ccatgcagca
    65281 atcacatcca taaacccttc gaacaacaaa atttctccgt ttttccggat atcgcttctt
    65341 gctttatcaa gattaaataa aacttgtctt ttattgaaca gttctgtttc tggactattc
    65401 aagtatttgg gctgatcttt ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa
    65461 ccgatcgtct ggccttgaga attacgaagt ggaaacatca ctctttgata aaaacgatca
    65521 gacatacggc catcatcatg cgtgacaaac aatccagagc gttctaacaa ttcatttgaa
    65581 attgattcat tcttgaatac ttgctgaaga aaagaacgct cattaggtgc aaaacctatg
    65641 ttgaattctt caattaattc taaagacaac ccacgacttt gcaaatattc taaagctggc
    65701 tgtccagctt ttgtatgcaa tagcatgtga tgatagattt ccgctgcttt ttcatgagca
    65761 gcaatcagtt tccccgtagc agattcagga tttcggacag ctatagtacg ctgacgccat
    65821 tgatcttcta acggtatctg ttcgaagtct gccactttta ccactgcttc tggaaaactt
    65881 agaccttcta gttcttgaag aaaagtaaac acgtttccgc ctttaccaca accaaagcaa
    65941 tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg tacgttcttc atgaaaagga
    66001 cacagcccca agtaattttt accagacttt ttcaactgga catattgtcc aatcacctca
    66061 acgatattcg tttgactgcg tactgtttcg ataacctctt gaggaatccg ttgagccatt
    66121 ttttcacccc ctgctctatt gatatctttt gggtttaaca gaagatattt caggagaata
    66181 accggtaaaa aatcgcacta actgaaatca gtgcgatttt aaaggacaca attatacatg
    66241 tttgattgta tcatatttca ttgctttttc aagtataaca catttctatc tcattaagca
    66301 tcaatttgct actcttttca gtaactgatt ttgaatatca gccttccagt acaatactcg
    66361 aagaacaata tttacgagca tgaatacaaa gaagtatcct acgctctctc ccaaccctaa
    66421 gtttacttcc aaaattccat ggaacagcat tgcagctaca tatatcccta cgatcgtcgt
    66481 tactgctagg aagatgcgca atgggttgaa gggataacaa gcttttacaa ctgccatgca
    66541 actgatccca atcagcaagt aatacatcaa agtagtagtt tcagcatttc cccatgcgaa
    66601 tgtttttcca agtagataca ccgcaatgat attcactact acaagcaagg catttggtaa
    66661 agcattaata agtgctgtag gcaaataacg gtaagtgatt tttcttttat cttgttcaaa
    66721 agataaaaag aaggaaggat acccttcaat cgctaaatca atcagcgtga tttggatagg
    66781 gataaacgga aatccaattg ctgtaacggc acatataatc gataaaataa atgaataaat
    66841 cgttttgata aaaaagatgc ctgagacttt cgtcacattg ttgaccactc ggcgtccttc
    66901 aaacaggact tctggcaatg tagtaaaatc agagtccaac aataccagat tcgcaatctg
    66961 acgtgttgct ccatcacctt ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa
    67021 aacatcattc acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt tcaattcatt
    67081 taccagcaat ttcttttgct gcggactcac tcgtccaaaa accgtatagc gatgagcagc
    67141 ttctcgcact tccatttctt cagtaatttg agaaagatcg acatacgcat cataattcgg
    67201 taatcctgca cgtctagcga tattggagac agtaaaagga ttgtctccag aaatcacttt
    67261 cagatccaca ccttcctcat gaagataagc tagtgtttct tgagcgtttt ttcggattgg
    67321 atcatcgatt tcaaacagag caaccggctc aacaaatgct ggtttttctt cgttcaattg
    67381 ctgatttgga gcgatcccca acataagtac gcgatagcca ttcgcttggg ctgtttttag
    67441 actgtccgga agccgatctt cttccaccaa acgttcaggc gctcctacgt acacgactcc
    67501 cagttctgga aattccatcg cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt
    67561 cacatcatac cgattagaaa gctcaaaata atctctcata gcccgcatag tgatgttgtt
    67621 atcactgctt tctgccagat acgtccccat aatttctggg atggctttct cgtacagcgg
    67681 atgtagtgct tctacccgct ggatactcat tttcccttca gtaatggtcc ctgttttatc
    67741 caagcataat gtatcgacat gggccagcgt ttcaattgaa tacatgtctt gaacaaggat
    67801 tcgttttttt gccagtttgg ttacccctgt cgttaatgca atacttatta gcaataccaa
    67861 accctttgga agcatgccta acaaagctgc tgcagatgca ataacagaaa ttttgataga
    67921 agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa
    67981 actagtgaat ttggatactt tgcgaatcga atctacaagt tcagaacgaa tagctttgtg
    68041 agtctttgcc tcgctagtca agcggacagc ataattatct gcacctactc ggataacttc
    68101 agccatgaca gaaccgcttg tcaaaaagct tccagaaagc aattcagctc cagtttcttt
    68161 gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt gcttctgccc gtccagatat
    68221 aacctgtaaa tcggctggta tctgttctcc tgctccgatt tttaccaaat ctcctaatac
    68281 cagctcttta gtagaaatcg attgttcttt accgtcccgg atcactagta cttctgattg
    68341 ggaaacaatc gataatttcc gtacaagatt tcttgcatgt atttcttgat aaattccaat
    68401 aacgatattg acgacaataa ttgctaaata cgccatgttg gaatacgcac ctacaaataa
    68461 cagacaaatc ccaataccaa agttcagaaa attgaataac gtcaatatat tatcgctgaa
    68521 tatttgtttt gtactttttg atgcatcttc ctggtagtca ttttgctggc cagaagccat
    68581 tttctgaact acctcttgct gtgtaagtcc attcattttc tttttctcca ttttccgcca
    68641 actttcaaaa ataatcacgt gtttaagtct agcgtgttcc aagattaaaa acaacttgtt
    68701 tttataaaat ttatagaaaa acacacgaat gattttacaa aaaagatgcc aacctttttg
    68761 tttgaatcac ccgtgtgctg tttttattat caatctatct ttacttatat tgttcctcca
    68821 aacggctcat ccaccaacct agtgctgtac cggctataaa aagaacagga caagctagta
    68881 ccccagcaac tcccaatccc gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa
    68941 ctacacctaa aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg taatccatga
    69001 tttttgacaa tccaagaaca caaagaatcc caccaattcc gatcggcaca atcacttcaa
    69061 gtcgtacttc tttaaatccg tcagccatcg ctttatacaa tcccatatac accaaaaaat
    69121 tagaagggct caatcctggg acgatcatac ccaaaccaat caatccgccg gcaatcagcc
    69181 atgttccaaa attctgatcg acttgcccgt caaataattg agcgccgtat aataaaaaca
    69241 aataagccag aacaaaagta acaaccatga tgatcacatc agaagtatct cttccttttt
    69301 tccctgcttc tttccataac gcaggaacag ttccagcgat acatccgaca aaaaaccata
    69361 atatcgttgt ttcatacgca cctaataaaa aactgactaa aaaagaaagt agaaagatgc
    69421 ctgtcactcc gcctagacca acaggcagaa aaaacaaaac attttctttg aaattttttg
    69481 taaggtgggc taaaaaagaa atcagccgct catacatgcc aaagacagct gctaatgcac
    69541 caccgctgac tcctggcaag ataaatcctg aaccgataaa cattcccttc acaaaccgta
    69601 acatccagtc tttggttgtg taccccactt cttgattttc cattttttgc cttttttcca
    69661 ctatttgcgt actccttctt tcatttaatt ttattcatga ttgaacattt cctgctattt
    69721 tacttaaaga ctttttcata ggtctctatt attttttcat tcattttgac aatatcagga
    69781 taaaacatgt tacctccatt atgatagaga tagccgccat tgtaaagcaa ggactggatc
    69841 cgccaatatc ggtattgttt cccttcctca tttcctaaaa gtgggctcag cacatcttta
    69901 gaatacgctt ctgctaattc tactgtgttt tctccgccat gattttttac ataggcaata
    69961 taatctttcc caaaattata tgcttggaca gctgtccata aatcgcatcc ttgtttcttt
    70021 gcttctacta tcatttccgc caaatatttg acaccttgat caatactgtc agctggatta
    70081 tcaatgatct cagcttctcc atgcagactt tccgagctct gcatgagatc tgtccctttt
    70141 ccttttgatt cggtcaatat tatcgccttg atcaaattct cgtagttttc catatgataa
    70201 ttttttgcag ctgttgttat ttctatttga tagctttcca ctcgttttac cgaacgataa
    70261 gttcttcctc ataaaaacaa caccgcacca ataaccgtca gaatgatcat gtaaaaaaga
    70321 atacggataa tagataatcg ttttcgcttc atttcatcac ctagttattt tgacatcttt
    70381 ttttatctta ctatttcttt atcttcttgg ctatttcttc cttgatttct gtgtacgcct
    70441 taagaaaaaa tgaattttcc tgtatttttt gacatcttac gaaaatgatc gttgtttttc
    70501 agtaaaaaca attgcccaat gactgatata atcgttatac tttacacagt aggtgattat
    70561 gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag
    70621 agcggtcaag gcttccctct ctttttactg catggcaatg acggcagcgg ccgctttttt
    70681 tcagaacagg tccctgtact cgaacgctac tataccgtgt atctagtaga tagccgagga
    70741 catggtcgtt cgacaaacga agcatctatg ttgaattttc agttaatggc cgaggatctg
    70801 aatacgatca tgttgctgga aaaaatcgat caggcagatt ttcttggatt tagtgatgga
    70861 gcaaatctag cactggtatt tgccagcagc ttccctaaaa aagttcatcg attgatttta
    70921 aattctggaa acacgcttgt taaaggcgtt cgtttttctg ccagagtcat cagtaatatt
    70981 cattatgcat gggtatggtt actctcttta tttcgcccaa gtcttcgcaa aaatctttta
    71041 gttatcaaac ttttgctgca tgacatcggg cttacagaaa atgatttaaa aaaaatcaac
    71101 tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat
    71161 atagctaaaa ctataccaaa agcttccttt gtgcttgtga aagaacaagg acatgaatta
    71221 gctagaaaag atcctgaacg cttcaatcga gaagttcttc aatttctttc tgaaacataa
    71281 ctattaggaa tagaaggtga tttatttgtt aaaaaatacc atacaatggc tgaaaaacca
    71341 cctaggactg ttcaaaacaa tctttttgat ttctgtcatt gtcattattg tcggtgaatt
    71401 gatgtccatc ggtaaaactc tatctatcca gcaattagga gaaacgtttg ccactatacc
    71461 tgtatggaaa agcggactaa tgctattgat tggtcttatc tctgtgttac ctatggttgg
    71521 ttatgatatt attttgaatc ggatgcttga acaaaagcag aatcctcgtt acctgtttga
    71581 gacaagctgg ctgatcaata cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt
    71641 ggggcttcgc tctgaactat atggacaaaa gaaagagagc agaaacgtga tccaagcatt
    71701 atcgaaagtt ttcttgtttt tgatggctgg actttctatt tacagtctgc tttctttttt
    71761 acttgtagtc ttcacgccag ttgctccttt tttgaagcaa tattggatat ggttgatcgg
    71821 cggcggtcta tattttcctg tcgtcttttt atttactact ttcaaaaaga aaggattcct
    71881 tggtggtacc tcacttaaaa ctcaaggaca gctattgcta gtttcattgt tggaatggac
    71941 cggtgtcctg tttagtttcc tttctgttgg ttatttaatg gaaatccgga ttgatctgtg
    72001 gcaaacaatt ccattattca ttgctgcttc agtgatagga atcgtgtcta tgattccagg
    72061 agaaattggg agtttcgatg taatgatgat tatcgggttg tccgctattg gtgtgccaag
    72121 agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc tactacatca ttccgttttt
    72181 gattggaatc gtctttttct tcaaaaatat cggttccact tttgatcagc gttattctgg
    72241 tatccctaaa caattagcaa cagaaattgc gcataaaatc gtggttgtct tattatactt
    72301 ttctggaatc atgctagtac tttctgccac tatcccgcaa gcttttacag agtttcgctg
    72361 gcttcatagt ttgaacccat tgaaatttca ttttattatc caatttccta gtatactatt
    72421 gggatttctt ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac gagcatattt
    72481 gcctacaatc ttcttgattg ttctagcttt actttatgtt ttattaagtg attttagttt
    72541 cacaccggtg gttttcttgt ctattctgct tctgatcatt cttgcttcta aaaacgaatt
    72601 atttcgggaa caattgatct attcatggga gtggcgtacg atcgatggaa tcattattgg
    72661 tgcactgtcc ttgctttaca tcgttattgg ggtatacaat ctgccggatt tccctcatcg
    72721 gcgtcaccat tttatttcct ttttcttatt tccttctgaa aagatttggt tttcgggatt
    72781 gttggccatc atcgctgtta gtttcatgat cgttctattt gttcatttcc ttcaaggaga
    72841 aaagaaacag attggcgaag cctttaatga agaaaaggcc ctgaagatcc ttacaactta
    72901 tggaggaaac tccgatagtc aattgatctt cttaaaagat aagcggatgt ttgcctatga
    72961 aaaagacggt gaaccaactg ttcttctcca attcgcttgc ttcaataata aatgtattgt
    73021 aatgggtgat ccttctggta agaaagaaga ttttccagaa gcaatcgaag cttttatcga
    73081 agaaacagat cgactatgct atttaccagt tttctacgaa acaagcgaag agattgttat
    73141 gattttgcat gaattcggct acgatttcat taagatgggg gaagaagctt atgtagacct
    73201 gaatagtttt acaacttcag ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat
    73261 cgaacgagaa ggatttacat ttgatgtctt acaaccacca ttttctactg agcaaatgag
    73321 tactttcaaa aatatctctg ataattggtt gggttcacga aaggaaaaag ggttttcttt
    73381 aggctttttc tctgaagatt atctacaaag agcgcctatt gcagttgtca aaaataccca
    73441 agaagaaatt gtcgcttttg caacgatcat gcctacttac acgaacaata aagtaggaac
    73501 gattgatctg atgcgatatg attctaaaaa agcaccttct ggcagtatgg atttcttgtt
    73561 cttgcatttg tttgcctata tgaaagaaga acatatccaa tggttcaatt tagggatggc
    73621 ccctctttca aatgtcggaa cttccagaaa gagtttttta caggaaagga ttgcatactt
    73681 ggtttacgag tttggttctc atttttattc tttccatgga ttaagagaat ataaaagcaa
    73741 gtacgctaca aactgggtct cacgttatac tctatattct agagatagtt ggattgcata
    73801 tgttatgatt gctttgctga ttattgataa tgcgccagtt gagcagacac aaggtagaat
    73861 aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg atagatgaac
    73921 actctcatga tgaataaaag agactgaagt aaaacgaaag ttcatgcttc tacaatctct
    73981 tcttatacaa ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg accgctcttt
    74041 tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc ttcgatctct
    74101 gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt
    74161 tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc
    74221 atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac
    74281 tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt tgtcaacacg
    74341 tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc aagtacttgt
    74401 ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt atccccagct
    74461 tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc tttttgtgtt
    74521 tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc acgatcaata
    74581 cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac tggcaatagt
    74641 aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt atccatcaat
    74701 tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc ggaacctttg
    74761 atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg aacttccatt
    74821 tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc
    74881 aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga
    74941 ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc tgtaatcatg
    75001 tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt
    75061 tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc tggagctgca
    75121 tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac tgtagtgatc
    75181 gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt
    75241 ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa ataatataga
    75301 tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact aaatcaaaaa
    75361 gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg tcaatattgg
    75421 tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct tttataaata
    75481 aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt aactttcttt tgaagcaata
    75541 agaacaaaat gaggaaaagt ttaaaattca gctgttgcca acaactttca atagacgaaa
    75601 tcttctattt cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta
    75661 cttgtcgttt taggcatatt tctcggcgga agttttgcag cttatcatta tttttacggc
    75721 ggcgaagctt attatacaaa aatcacgacg aatggcgaaa tgtcagctac ccaagcagat
    75781 aacggagaga aattcacgac ttatacctac aagcaagcgg cttataataa agatggtgaa
    75841 aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat taaagatgaa tgcgtatctt
    75901 aaactcaaag tcaatcctcg taaaggtgta ttaagctggg aagaaataaa ggcttctgaa
    75961 gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa attttattag
    76021 ataaaa
//
LOCUS       DAZKCC010000004        68954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000004 DAZKCC010000000
VERSION     DAZKCC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.004"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..383
                     /locus_tag="ACG8VH_000270"
     CDS             <1..383
                     /locus_tag="ACG8VH_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFP9108866.1"
                     /translation="LESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFIL
                     NHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVESN
                     YKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(688..1002)
                     /locus_tag="ACG8VH_000271"
                     /pseudo
     CDS             complement(688..1002)
                     /locus_tag="ACG8VH_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(1256..2891)
                     /locus_tag="ACG8VH_000272"
                     /pseudo
     CDS             complement(1256..2891)
                     /locus_tag="ACG8VH_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3214..3693)
                     /gene="rlmH"
                     /locus_tag="ACG8VH_000273"
     CDS             complement(3214..3693)
                     /gene="rlmH"
                     /locus_tag="ACG8VH_000273"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9108867.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4121..5410
                     /locus_tag="ACG8VH_000274"
     CDS             4121..5410
                     /locus_tag="ACG8VH_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP9108868.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(5449..6054)
                     /gene="ytpR"
                     /locus_tag="ACG8VH_000275"
     CDS             complement(5449..6054)
                     /gene="ytpR"
                     /locus_tag="ACG8VH_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP9108869.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(6259..6723)
                     /locus_tag="ACG8VH_000276"
     CDS             complement(6259..6723)
                     /locus_tag="ACG8VH_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9108870.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(6745..7065)
                     /locus_tag="ACG8VH_000277"
     CDS             complement(6745..7065)
                     /locus_tag="ACG8VH_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9108871.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(7209..8288)
                     /gene="pepA"
                     /locus_tag="ACG8VH_000278"
     CDS             complement(7209..8288)
                     /gene="pepA"
                     /locus_tag="ACG8VH_000278"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP9108872.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            8488..8838
                     /locus_tag="ACG8VH_000279"
     CDS             8488..8838
                     /locus_tag="ACG8VH_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9108873.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            9061..10008
                     /locus_tag="ACG8VH_000280"
     CDS             9061..10008
                     /locus_tag="ACG8VH_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9108874.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(10065..11483)
                     /gene="pepV"
                     /locus_tag="ACG8VH_000281"
     CDS             complement(10065..11483)
                     /gene="pepV"
                     /locus_tag="ACG8VH_000281"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP9108875.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            11632..12477
                     /locus_tag="ACG8VH_000282"
     CDS             11632..12477
                     /locus_tag="ACG8VH_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP9108876.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12481..12921
                     /locus_tag="ACG8VH_000283"
     CDS             12481..12921
                     /locus_tag="ACG8VH_000283"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9108877.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12971..13165
                     /locus_tag="ACG8VH_000284"
     CDS             12971..13165
                     /locus_tag="ACG8VH_000284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108878.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            13301..13987
                     /locus_tag="ACG8VH_000285"
     CDS             13301..13987
                     /locus_tag="ACG8VH_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9108879.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(14395..15084)
                     /locus_tag="ACG8VH_000286"
     CDS             complement(14395..15084)
                     /locus_tag="ACG8VH_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108880.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(15059..17188)
                     /locus_tag="ACG8VH_000287"
     CDS             complement(15059..17188)
                     /locus_tag="ACG8VH_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP9108881.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(17236..18573)
                     /locus_tag="ACG8VH_000288"
     CDS             complement(17236..18573)
                     /locus_tag="ACG8VH_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP9108882.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18825..19184)
                     /gene="rplT"
                     /locus_tag="ACG8VH_000289"
     CDS             complement(18825..19184)
                     /gene="rplT"
                     /locus_tag="ACG8VH_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9108883.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(19237..19437)
                     /gene="rpmI"
                     /locus_tag="ACG8VH_000290"
     CDS             complement(19237..19437)
                     /gene="rpmI"
                     /locus_tag="ACG8VH_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9108884.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(19509..20033)
                     /gene="infC"
                     /locus_tag="ACG8VH_000291"
     CDS             complement(19509..20033)
                     /gene="infC"
                     /locus_tag="ACG8VH_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9108885.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(20077..20202)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            20353..20985
                     /locus_tag="ACG8VH_000292"
     CDS             20353..20985
                     /locus_tag="ACG8VH_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9108886.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20987..21892)
                     /locus_tag="ACG8VH_000293"
     CDS             complement(20987..21892)
                     /locus_tag="ACG8VH_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9108887.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21977..22636)
                     /locus_tag="ACG8VH_000294"
     CDS             complement(21977..22636)
                     /locus_tag="ACG8VH_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9108888.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22952..23896
                     /gene="mvk"
                     /locus_tag="ACG8VH_000295"
     CDS             22952..23896
                     /gene="mvk"
                     /locus_tag="ACG8VH_000295"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP9108889.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23889..24866
                     /gene="mvaD"
                     /locus_tag="ACG8VH_000296"
     CDS             23889..24866
                     /gene="mvaD"
                     /locus_tag="ACG8VH_000296"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP9108890.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24879..25964
                     /locus_tag="ACG8VH_000297"
     CDS             24879..25964
                     /locus_tag="ACG8VH_000297"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP9108891.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25961..27025
                     /gene="fni"
                     /locus_tag="ACG8VH_000298"
     CDS             25961..27025
                     /gene="fni"
                     /locus_tag="ACG8VH_000298"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9108892.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(27274..28077)
                     /locus_tag="ACG8VH_000299"
     CDS             complement(27274..28077)
                     /locus_tag="ACG8VH_000299"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9108893.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(28097..28939)
                     /gene="kduI"
                     /locus_tag="ACG8VH_000300"
     CDS             complement(28097..28939)
                     /gene="kduI"
                     /locus_tag="ACG8VH_000300"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9108894.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28955..29962)
                     /locus_tag="ACG8VH_000301"
     CDS             complement(28955..29962)
                     /locus_tag="ACG8VH_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP9108895.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29969..30613)
                     /locus_tag="ACG8VH_000302"
     CDS             complement(29969..30613)
                     /locus_tag="ACG8VH_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9108896.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(30713..31471)
                     /locus_tag="ACG8VH_000303"
     CDS             complement(30713..31471)
                     /locus_tag="ACG8VH_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9108897.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(31598..32749)
                     /gene="fucO"
                     /locus_tag="ACG8VH_000304"
     CDS             complement(31598..32749)
                     /gene="fucO"
                     /locus_tag="ACG8VH_000304"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9108898.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(32831..33142)
                     /gene="rhaM"
                     /locus_tag="ACG8VH_000305"
     CDS             complement(32831..33142)
                     /gene="rhaM"
                     /locus_tag="ACG8VH_000305"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9108899.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(33144..33989)
                     /gene="rhaD"
                     /locus_tag="ACG8VH_000306"
     CDS             complement(33144..33989)
                     /gene="rhaD"
                     /locus_tag="ACG8VH_000306"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9108900.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(34003..35259)
                     /gene="rhaA"
                     /locus_tag="ACG8VH_000307"
     CDS             complement(34003..35259)
                     /gene="rhaA"
                     /locus_tag="ACG8VH_000307"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9108901.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(35256..36716)
                     /gene="rhaB"
                     /locus_tag="ACG8VH_000308"
     CDS             complement(35256..36716)
                     /gene="rhaB"
                     /locus_tag="ACG8VH_000308"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9108902.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36869..37738
                     /locus_tag="ACG8VH_000309"
     CDS             36869..37738
                     /locus_tag="ACG8VH_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9108903.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(37864..38763)
                     /locus_tag="ACG8VH_000310"
     CDS             complement(37864..38763)
                     /locus_tag="ACG8VH_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9108904.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38928..39752)
                     /locus_tag="ACG8VH_000311"
     CDS             complement(38928..39752)
                     /locus_tag="ACG8VH_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9108905.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(39763..41058)
                     /locus_tag="ACG8VH_000312"
     CDS             complement(39763..41058)
                     /locus_tag="ACG8VH_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9108906.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(41074..41553)
                     /locus_tag="ACG8VH_000313"
     CDS             complement(41074..41553)
                     /locus_tag="ACG8VH_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9108907.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(41559..42587)
                     /locus_tag="ACG8VH_000314"
     CDS             complement(41559..42587)
                     /locus_tag="ACG8VH_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP9108908.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(42604..43710)
                     /locus_tag="ACG8VH_000315"
     CDS             complement(42604..43710)
                     /locus_tag="ACG8VH_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9108909.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43939..46536)
                     /gene="adhE"
                     /locus_tag="ACG8VH_000316"
                     /gene_synonym="adhC"
     CDS             complement(43939..46536)
                     /gene="adhE"
                     /locus_tag="ACG8VH_000316"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9108910.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(46841..47221)
                     /gene="yajC"
                     /locus_tag="ACG8VH_000317"
     CDS             complement(46841..47221)
                     /gene="yajC"
                     /locus_tag="ACG8VH_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9108911.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(47318..48463)
                     /gene="tgt"
                     /locus_tag="ACG8VH_000318"
     CDS             complement(47318..48463)
                     /gene="tgt"
                     /locus_tag="ACG8VH_000318"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9108912.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            48681..49379
                     /locus_tag="ACG8VH_000319"
     CDS             48681..49379
                     /locus_tag="ACG8VH_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9108913.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(49547..52186)
                     /locus_tag="ACG8VH_000320"
     CDS             complement(49547..52186)
                     /locus_tag="ACG8VH_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9108914.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(52805..53836)
                     /gene="queA"
                     /locus_tag="ACG8VH_000321"
     CDS             complement(52805..53836)
                     /gene="queA"
                     /locus_tag="ACG8VH_000321"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9108915.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            54029..55203
                     /locus_tag="ACG8VH_000322"
                     /pseudo
     CDS             54029..55203
                     /locus_tag="ACG8VH_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            55207..55848
                     /locus_tag="ACG8VH_000323"
     CDS             55207..55848
                     /locus_tag="ACG8VH_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9108916.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            55845..56762
                     /locus_tag="ACG8VH_000324"
                     /pseudo
     CDS             55845..56762
                     /locus_tag="ACG8VH_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            56759..57430
                     /locus_tag="ACG8VH_000325"
     CDS             56759..57430
                     /locus_tag="ACG8VH_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9108917.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57971..58459)
                     /locus_tag="ACG8VH_000326"
     CDS             complement(57971..58459)
                     /locus_tag="ACG8VH_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP9108918.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(58660..58926)
                     /locus_tag="ACG8VH_000327"
     CDS             complement(58660..58926)
                     /locus_tag="ACG8VH_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9108919.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58926..59189)
                     /locus_tag="ACG8VH_000328"
     CDS             complement(58926..59189)
                     /locus_tag="ACG8VH_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9108920.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            59710..59850
                     /locus_tag="ACG8VH_000329"
                     /pseudo
     CDS             59710..59850
                     /locus_tag="ACG8VH_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(59992..61815)
                     /locus_tag="ACG8VH_000330"
     CDS             complement(59992..61815)
                     /locus_tag="ACG8VH_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9108921.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(62413..65118)
                     /locus_tag="ACG8VH_000331"
     CDS             complement(62413..65118)
                     /locus_tag="ACG8VH_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP9108922.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(65424..66371)
                     /locus_tag="ACG8VH_000332"
     CDS             complement(65424..66371)
                     /locus_tag="ACG8VH_000332"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP9108923.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            66561..66830
                     /gene="rpsN"
                     /locus_tag="ACG8VH_000333"
     CDS             66561..66830
                     /gene="rpsN"
                     /locus_tag="ACG8VH_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9108924.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(67006..67368)
                     /locus_tag="ACG8VH_000334"
     CDS             complement(67006..67368)
                     /locus_tag="ACG8VH_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9108925.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(67379..68500)
                     /gene="alr"
                     /locus_tag="ACG8VH_000335"
     CDS             complement(67379..68500)
                     /gene="alr"
                     /locus_tag="ACG8VH_000335"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9108926.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(68516..68866)
                     /gene="acpS"
                     /locus_tag="ACG8VH_000336"
     CDS             complement(68516..68866)
                     /gene="acpS"
                     /locus_tag="ACG8VH_000336"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9108927.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT        19855 a        14647 c        12564 g        21888 t
ORIGIN      
        1 aattagaaag caactttttt cgggatctaa atgaaattct ctcttatgat ccagcccata
       61 gctatgagga actatacact cgcttgattg actatgttta cgcaaatact tttttatatc
      121 gcttatttat cctcaaccat ggtgatattt cgtttcagaa gaaagttaaa caagttattg
      181 aagaaaacta tctgaacata tggctatatg aagacaagaa aacgattatc acagaagaaa
      241 tgagattctt tactacgtac cacgtccaag gttgtctatc cgttctcaat cggtgggtag
      301 aaagcaatta caagtactcc aaaaaagaat tattagaact cttacagaaa ctgaatgatc
      361 acatagagaa aatcatgcct tgaataaaaa catactaagc ttagttaaga agaaccgtaa
      421 ccttctcgta taacaactga atcttattta gccaaattgt ttactgaaga ttttttgagt
      481 aaaatttcta taattcctac ttattgaaaa gtagtttgta gtgctgcttc tccctctcta
      541 agtgatatat cttgttgtac caatcaaatt cacctcatca ttaataaatt acttttaggc
      601 taatttatta caacttatag gttgaagctg taaccaattt atagtcatat tatattttta
      661 tcatccagtt tattgaattc ttctcgttta cctcaattca ttttcaaggg gaatcagctc
      721 atcatctaac attttacgcc atgctcgctt catttcttgt gggtatttaa atggtttcgc
      781 ttctggatgt ttggctttat atccttcgta atccttactg ttgataattg ctttcatatt
      841 aggaattgga tcttcacctg gcatatattg cagattagaa gcatgtaatt ttttctcaga
      901 aacaaaccac tcattggaaa agacatgaat caaatcatca cttacttgtg taaaatagcg
      961 acgtttaatg acaaagatat cttcattgct atctagtgta ccttcatttc tttgtgctta
     1021 aattcaaaga taattttctc aataaagatt tttgattcca gtaagattcc tttactcctt
     1081 ttacaacata gcgttgtcct ttgttttgaa acatcttctt cccctctttg ccattaaaaa
     1141 tagcccaatt ctctataaag aagattaagc ttcattatta taatataaag tttaaatttg
     1201 gtttattact tactttttct tattctctag taagctaata ttgaaaaaaa tgtaatcaga
     1261 tcattatttt tatatgttgt aacttcctga taagaaaact aattaaaaga aagaggtaac
     1321 tacataaact aattaaaaaa gctaacttca tagttggagg ataaatggaa gaaacttcaa
     1381 tgattgtttc tccttgtgtt ccttcaatcg acactagtcc tattatcaat ctctcttttt
     1441 tgtccattta cttttgcgac tgttccccaa taatcaaata caggaagcgt tattttttgt
     1501 cctttagatt catgtactgt atagactatt ttcgaatcca tcacttgata aggcatagta
     1561 tgccattcct tgccaattct tacttcatga ttctgcaaat gttctttgta ttgtaatgct
     1621 tccgtgggtg catagtcata aagtcctccg ggaaggagtg tcgtgtaacg aataagattt
     1681 tcttgattca ttaccaagtg atctctctga gtgacaactt tatgttcata gtttgctttt
     1741 gttgcagcag atacagccaa cagtaataaa acaattgccg ttatattaac tacttttctt
     1801 tgcttagctg atgtgctctt catgaaaacg aacgataaaa tcaacgagcc aaacagtact
     1861 tgaaagccta agattcgcca aggaaattga atcatttgaa tgggtgtatt ttgtaataaa
     1921 gaccatggaa aaaatttggt ggataagagc caagagacta ccgcactaag gtaaatcact
     1981 ttttctttgc gattaaatcc tttaaaaaag aagagtggac agatcaaaga aataatacca
     2041 atgattccta ccgtaaaggt tgtcaggtca ttattcatcg caccaatcaa actatccgag
     2101 acatttaatg cttggtgttg taaggtcgtt ttatctggct cattaatcga ttgaaccatg
     2161 atttgtcgga acatcggata ccaaaagtat gctgttaaaa gaatcgtcat tcctactgcc
     2221 tttgacaact ccacaatcaa ccggatgctt tctttttttc ttgtttcact tactacaaag
     2281 ccgcctaaga ctagtaagcc aacggctatt acactcataa aagtggaaag aacatgtgta
     2341 taaatgagta agctcatact gactgctaat ggaattgcac atggcttttt ctcataaatc
     2401 agtgcgtaag cataatacat aactatcggt ataaccgtaa gtgcaatcgc ttcagcaata
     2461 gcagaacgat gataaatatc aatcgtacgg tacccataaa atgtatataa acagctaaat
     2521 ataaaagccg catcgattct ttttaaaaat tttagtccat aatgaaacat cagcatgata
     2581 gtacaaacat tcaatagcca aacatataag atataactaa cgattaaatt attacttatc
     2641 caataaaaaa tgactgccgg aaaaaaggtc aagtagggat aaaaataatt cactccgctc
     2701 ccataatgat taaacgtcgt aaaattgatt ggaccagaaa aaattgaaga taaccctttg
     2761 actctagcaa aatggaaggc aatatccccc gatgtagaaa tagtatgaac atttctaaaa
     2821 taattgattg aataaagtcc agcaataatt gctaggagaa tggacaatat tatttttttc
     2881 tttacttgca ttacttaatt atatactcct ttcatggatt gctttatcta gtataactta
     2941 aattttaaat aaatcgttat ttatttttac tctcacttaa aaaagcacta aaattataag
     3001 agagggaggt aatggtctga acaccaagaa ttgttccaac atcgaattgc ttaactaatg
     3061 agttcaaagc ttaattatgt ggctcttata tttaccgagc gtggattgcg atctctttag
     3121 cgaaacaaac atctttcata aaaaaaacag ccatatccgc tttcttggat atgactattt
     3181 actactactt tactatcttt cattcttctt gtctcactta tgataaggcg cccccgcatt
     3241 gatccggaag gcacggtaga tttgctcgac aaggatcaga cgcatcaatt gatgtggata
     3301 cgtcattttt ccaaaagaga tctgagcatt gcttcttttc atgacggctt cgctgagtcc
     3361 taaagagccg ccgataacaa aaacaaggtg gctttttcct tggataccta attggtcgat
     3421 ttgtttagca aaagattcac ttgtaggatt tttcccttca attgctaagg catagacgta
     3481 ttcttgttct ttgattttcg ctaagatccg ttccccttct ttttctttta cttgtctcat
     3541 ttgcgcttcg cttaaattct ccggggcttt ttcgtcagga acttcgatca attcgatttt
     3601 tgcataggca ttcagccgtt tgacatactc attgatacct tggatcaggt atttttcttt
     3661 taattttcct actgagataa ttttgatatt catgttttct ctcctttggt cttcatgctc
     3721 actttgttta ttataatcaa ttttttcgtc tatatgttta ttttggctat ttttgttaaa
     3781 aagatttccc caacagttat gcacaacatc cacaggttta tccacagaaa acagtgttat
     3841 tttaccttat ttcatcacat tcattaggca aacatacgtt ttataagaat ttatccactt
     3901 tatacacagg ctgtggatat gttttatccc ttatttttcc aatcctatcc gcgtagtttt
     3961 acacagtatc tgtggaaaaa gaaaatcaaa tattttcatc tgaaaacaat gatttttcct
     4021 aaagtttttc ataaaaagtt cacagtctca aagtagacta aagtcaacaa ataagactat
     4081 actaacagta gataaatgaa tgtaattgga ggaggaacac atggatagaa aaaatgtgac
     4141 accaaagatg aaaaaaagta aaaataatag cctctggcgt aagttaggtc tggggcttgt
     4201 tggagggatc gttggaggac tactgacagc tggcattttt tatgcagcga tgggcactgg
     4261 caacaatagt tctactgcta caagcggcaa tcagaattct gccggtgaaa cagttgtgga
     4321 aaatgtcaaa gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc
     4381 tgtcgtttct gttatcaact tacaaagtca aaaccaatca agcggttttg gcggactatt
     4441 cgggcagcaa gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg
     4501 aagcggcgtg atctacaaaa aagacgggaa tactgcttat gtagtcacga ataatcacgt
     4561 agtagatggc cagcaaggtc ttgaagtctt gatgaaagac ggtacgaagg taaaagctga
     4621 attagttgga acagatgctt attcagattt agcggtgttg aagatcaatt cagacaaagt
     4681 cgaaacagtt gcctcttttg gtgactcaag tgctctgaag gttggtgagc ctgcgattgc
     4741 gattggttcc ccactaggat ctgaatatgc aaattctgtc acttcaggaa tcatttcttc
     4801 cttgaaccga caagtgacaa gtacgaatga atcgaacgaa acagtcaata tcaatgcgat
     4861 ccaaacagat gcagcaatca accctggaaa ttctggtggt cctttagtta atatcgaagg
     4921 acaagttatc ggtatcaact caagtaagat cgcaagtact tctgaatcta cttctaatgt
     4981 aagcgtagaa ggaatgggat ttgctattcc aagtaacgat gtagtgaata tcatcaatca
     5041 actggaaaaa gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc
     5101 tgccgtttct actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg
     5161 tgtcatcgtg acaaatgtcc aaacagctac accggctgaa aaagctggat tgaaacaata
     5221 cgacgtcatt actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc
     5281 tttgtataag aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga
     5341 aaaaacaact actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag
     5401 tgaaaattaa taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa
     5461 tgcttctccg actacggcat cctcaggcaa tactaagatt cctttttttg ctggagcatt
     5521 tggcaaatgc aattcacgag cggaacagat cattccatag ctatctaccc ctcgcaaagt
     5581 tcctggccag atcatcattc catctggcat catagctcct ggttttgcaa cgacaacttt
     5641 ttgtccagct tcgatattgg gtgcaccgca aacgatttgc aatactgtac cattgtctac
     5701 ttctgtctca gtaatcgata aatggtcaga atcaggatgt gggacgcatg tttttacgta
     5761 cccgacaacg atcttcggtt catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt
     5821 gatcaatcga ttcaatttcg ctaattcctc atctgttaag cttacttggc cgttcccttc
     5881 gattgttaga tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc
     5941 tttgatccga gcaacattcc atttgcgttc aacagtgttt tctgttcctt tatcgtcggc
     6001 tataataacc atcaaggtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt
     6061 tcctccatta tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgaaat
     6121 ttttttcatg ctttatttcc gaagaagcct cttctggaac accatttatt cgtaacaagg
     6181 gactgtgaca agacttttgt cacagtccct ttactgtttt agttaattga ttaaaaaggt
     6241 cgtacaagtt ccacttgctt aattacttgg ctgttcttct tcgtcagaaa cgatcaagac
     6301 atcacaaggt gaatgacgta tcacgtagct ggctacactt cctgtcataa agcgttcgac
     6361 tgcatttaga cctgactgcc caaccatgat tagatcgatc tcatattttt ttggcaattt
     6421 gactgccatg gattcacgaa cagaaccata tgcaataatt ccttctatat ttgtgtaatc
     6481 aacagatttc ccatattctt tacattcttc gatcatttcc ttggctgttt cagtttgctg
     6541 atcgactaga ttttgatcaa taggtgcata gcccataaag ttataaaatt gctgatcgat
     6601 tacgtttgct acatatactt tcccattatt acggcgagcc acttctaccg cttttttgaa
     6661 agcagcaagt gcttgttcac taccgtcgat acctactaaa atattcttat attcttgatt
     6721 catcttgacc cctcctaaca gatgtcagcc ttttgttaca ctttctagga acgattcgat
     6781 ttcctgtttt gttttacgat ctttattcac tagacgacct aattcttttc cgtcttggat
     6841 cacgacaaaa cttgggatac caaaaatgtt ccattcagcg gctacatcta tgtacttgtc
     6901 acggtctact tcaataaatt gccaatcaga aaagtcattt tcaatttcag gcatttgcgg
     6961 tttgataaaa cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac tctttccagt
     7021 ttctacgtag cctgctaatt cttctaaact atttgggata atcattcttt gtcctctact
     7081 ttctgtttat gtatttttca attaaattat acacctttct ttcgaactaa caaacattga
     7141 tcctcttttt ataaaaaaaa tctggcaaaa agcaatcttt tgccagattt tatagtttca
     7201 ctatcgcatt attttccgta tacgatcgta tttactgttg atttatccaa acctttcagt
     7261 gtttgaatca acatttctcg tgcagcttca aaatcgcgaa tactgaacat cgtttgatgc
     7321 gtatggatat aacgtcccac tacgccaatc actgtacttg ggattccttc gttttgtgta
     7381 tgagcagcac ctgcatctgt accgcctttt gaaacaaagt attgataagg gatattgttt
     7441 gtttcagcga tatccagcaa gtactcgcgc aaacgaggaa gcatgatcaa acctggatct
     7501 tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt cgtcactgta
     7561 tcatctgctg cagagcagtc tactgcaaag aacagatctg gcttgaattt attgacagaa
     7621 gctttagaac cacgcaagcc tacttcttcc tggacattcg cgcctgcaat caatgtatga
     7681 cctaatgttt cattttgcaa cgcttctaaa gcttctaata ccattgtaca gccatagcgg
     7741 ttgtcccatg atttactgat aatattcttt ccgttggctg ttttgatcgt ttcagcgtaa
     7801 ggaacgattg tgtctcctgg aaggacaccg taactcattg cctcttctct tgattcaaac
     7861 ccagcatcga acaaaatatc ggttacttct actgattttt gtccgcttgt tcctcgcaat
     7921 aaatgtgggg gaacggaaga agaaatacat ggataattct taccagtaga ggttttaagt
     7981 gtaaaacgct gagcagaaac aacgtaagga ttccagccgc ctagaggtac gactttaaaa
     8041 agcccgtttt cttggatttg tgtcagcatg aatccgactt catccatatg tgctgcgacc
     8101 attacacggg gtgcatccgc ttcttcatga tggcgcagac caaaaatgcc gcccaaacca
     8161 tctaactgta actcatctac cagcggttcc atatgacttt ccatataagc gcgaacatct
     8221 tgttcaaaac cgcttgttcc ttgtaattct gttaactctt tgattcgttg aaatgttttt
     8281 tcttccatat gctattcgtc taaatttcag actaaactcc tctcagactt atattgatct
     8341 gcggataaaa tatgattttt tctgcagacc gatcgtattt attatagctt attttttctg
     8401 gtagcggtta tttatttcaa agaaaatatg agagtgtggt aaaataagca caaggagtaa
     8461 caagtgagaa gaaggaggta tgtccacatg tatgaagaaa gcgaactaag ctattttaaa
     8521 ggcgggttag cagttggaat cggtctagga ttattgagcg gggtagcttc aacgatttgg
     8581 tatcatcgca aaaaagtatt gtcagcagac caagtacttg aaaatatcaa gtcatctttt
     8641 ctaaaagaag gtgcaatcga aggttcttgg atctcttttg aaaaggaacc tattaggaaa
     8701 ttcgccgttc ggtcgagtgc ttatcgggga ggtctttctc gaatagaaga tggcgaaact
     8761 gtttattatg aatttttagc agatgctcat acaggaaccg tattagaaat ttcaagaaaa
     8821 aaaggtgcaa cagattaata cgtcaaaaaa gtgagagaac gaatacgcac ccactattca
     8881 aaagagaggt atcatgtcct tcttttgtag tgggtggttt tttagataga gctttccaaa
     8941 catatattcc agtattttct ttttaaataa atgatattta gcgggaaaaa ctagcatttc
     9001 ctttgattat gaggtatttt taatttagct tttgttttct atagttaagg agagtgaaaa
     9061 atggaacaac aagaaaaaat tgctgttctg attccttgtt acaatgagga agcaacaatt
     9121 tcaacagtca ttgcagactt taaacgagaa ttaccagaag cagatattta tgtctatgat
     9181 aataattcga ccgatcgaac atacgaattg gccgttcaag gtggagcaat cgtaaaaaaa
     9241 gaacctcgtc aaggaaaagg aaatgtgatc cgccaaatgt tctttgatat cgatgccgac
     9301 tattatttaa tggtagatgg cgatgatact tatcctgcag aagctgtaca tggtttgctc
     9361 gagaaattac gttctggtga agcagatatg gtcattggtg atcgcctatc aaatggaacc
     9421 tactttgatg agaataagag acctttccac gattttggaa ataaccttgt tagaaataca
     9481 attactagaa tgtataaaac aaaaattctt gatgtcatga ccggctatcg aggattcaat
     9541 cgtatctttg tcaaaagttt tcctattatg agttctggtt ttcaaatcga aacggaattg
     9601 acgatccatg ctttagataa aaaattcaag ttcgttgaaa tcccaatcga ttatcgtgat
     9661 cgtcctgaag gcagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     9721 atgattatta aaatgtttaa agattataag cctttgcttt tctttgggat ttggaccatc
     9781 ttcttctttt tatttggatt ggtcacgggg attcctgtga ttcgcgaata tatgctgaca
     9841 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagcactt
     9901 ctttcattag taacagggtt gattttggat acagtcgtca cgaacgctaa gaaagaatat
     9961 gaattgaatc tatatcatgt ttatcatgaa taccacagta gaaagtaaaa aaaggatcaa
    10021 gactgcaaaa ataatttgca attcttgatc ctctttttaa ttaattattt cgccaaacga
    10081 tagatggcat ctgcatagat tgctgttgct ctgaacaagt catcaagact cataaattcg
    10141 tttgcttgat gcattgtatc agtatatcct gggaacatcg ctccataagc aacgccacgt
    10201 ttcaacaaac gtccgtaagt tccgccaccg atgatttgtt cgtagccttt ttctccagtg
    10261 tggtcttcat agacttgcaa taatgtagcc accaatggat catccattgg tacgtaatgt
    10321 ggttccatta cgcgagcacc tcgagtaact gttgcgcctt cttgccccac tgtttgttcg
    10381 atgtcacttt gcaatccgtc tactgttaca ccttttggaa aacggaaatt catattgatg
    10441 aagttcgctt cctctgtacc attttcttca aaagcaaata gtccagcatt cattgtcaat
    10501 ttgcccattt tttcatcttc ataagcgact cctagttttt ctccataaaa atcttcatgg
    10561 acataagcag ctgctacatg gataaattgt ttagcagatc ctaggaattc atagttatct
    10621 aagaacaatg ccaagaaaga acctgcattg ataccagatt gtgggcttgc accatgtgcg
    10681 cctttaccaa ctaattcgat ttttacatac gtattgtccg cttcaatcgt tccgcttact
    10741 ggatttgctt cgatgaattg gtaaaatgct tcttccatcg cgatagctgc atctgcactt
    10801 ggtacttgaa gtgctgcagt tgctgttcct ggcaccatat tttcgcgaag tccggaaaca
    10861 aaagatttca agacataatc tcctgcattg tcgcctttga agcgtaaagc taaagaaaca
    10921 tttccttttt caccattgat gattgggaat tcagcatctg gtgagaaacc aaaatctggt
    10981 tcttcttcat gttcgaaata ataagccata tcgccccagc cgctttcttc atcgctgccg
    11041 actacgaagc gaactttttt tgatagtttc aagtcaagtt ctttaatgat cttcatcgca
    11101 tagtaagctg ccatacttgg acctttgtca tcgcttgatc cgcgtgcaaa gatctttcca
    11161 tccttgatta ctggttcata aggatctgtg tcccagccat ctcctgcagg aacgacatcc
    11221 atgtgtccaa atatccctag cgtttcgtct ccttcgccaa gatcaatatg ccctgcatag
    11281 ttatcgacat tttttacttt aaatccatca cgttcgccat aagctaacat atgtttcaat
    11341 gcgtcgcgag gtccaggtcc aaatggtgca tctggtgtta cttttgagtc atcgcgctcg
    11401 ctgttgacac gtaaaagatt tttcaagtct tcaagcaagt cttctttacg tgcttctact
    11461 tctttttgcc aatcaattgt cataaagtta atcctccact tatttaagtc ttcgtgttca
    11521 tgataccaca aattacagaa aaaaatagtt caaaaaatct gacaattcct tttcttatct
    11581 gtttttctct aaaaaaaata tacgctataa tgagtaaaag gaggttttta gatgctgata
    11641 aacgaacaac tagtgaaaag agtcatcaca cctcgtccaa aaatttcaca taaaggaaca
    11701 tttggacgta tcgttttgat tggcggcaat gaacaatatg gcggagcaat cattatgagt
    11761 gctttagctg ccgtacacag cggagcgggt ctcacgactg tagtaactga tgaaaaaaat
    11821 cggagtgctc ttcacagtca tctgccagaa gcaatgattg tagtttggac ggatactgac
    11881 cagtttcttt ccgtaataaa gcaagcagat gtactcttga ttggtcccgg attgggtact
    11941 tcttctaaga gtttggaacg tttcaaacaa gtactagaaa accaatcaaa agaacaatgg
    12001 ctggtgattg atggctctgc tttaagttta cttgctgcta atcatctttt gctaccctat
    12061 cccgaacaaa ctgtacttac acctcatcaa atggagtggc aacggttgag ttctctaccg
    12121 atacaagaac aacgggataa agaaaacaaa aagaagcaac aggaaatcgg tgctgtgctc
    12181 gttttgaaaa gtcatgagac aaaaatttat ggtcaccagc acttctacac caatccttta
    12241 ggttctgctg ctatggcaac gggcggcatg ggtgacactt tagctgggat gatcacagga
    12301 tttttagccc agtttcctca aaatgaccaa acgattgcag cagctattta tcttcatagt
    12361 ttcattggag accagctggc aaaagaacaa tatattgttt tgccgacaca aatcagcgaa
    12421 gcacttccga aatggatgaa ttatttttgt aaaaaatcaa tggaaaacga ggcatgagtc
    12481 atggagaaat ttacaggtta tgtgcgagaa cctttttatt atgaaacaga tcaaatgggg
    12541 attattcatc acagcaatta tatccgctgg tttgaagagg ctcgggtagc cttattggta
    12601 catctagggt ttccctataa aaaaatcgaa gaatctggga tcatcatccc cgttctggaa
    12661 gtaaattgca gctataaaga aatgatccat tacggagaaa aagtgagcat ccatccaact
    12721 atccaaaagt atacggggac acggttggat tttagctacg aaattgtggg aactgaagac
    12781 aaaaaattaa aaactactgg cagtagtaaa cactgttttt tactttctga ttcgcaacga
    12841 ttagtgaagc tgagcaaagt caatccggag ttagatcagt tattccaagc gtacgcttct
    12901 ttggatgatg taaattctta ataaagaccg tctaagcatt tttcttgtat tgtgtcgagt
    12961 agttcgtata atgaaaagaa aaaagcaggt gatccccatg tatgtcgtta aagtaatgca
    13021 cggttacatc gataaaactg gctgtcgcac tcgggaaaaa aacttagaca atctgctcat
    13081 tttcaaagac aaaaaagaat ccgaagcttt tgctaaaaga atcggcggtc gtgtcaaacc
    13141 gatccaagag gtacgaccag attgattcaa tatttttctt ttcgaggctc taataggcat
    13201 gcctatcagg gctttttttg cagactgtgt ttttatgccc acacgatttg taagcgattt
    13261 atgttataat agattatatc tgcatagaaa ggaaggttaa atggttatca ataatttgca
    13321 acagcaagct tatgaatcta tacgtaaaca aatcatttat tccgagttag cacctggaag
    13381 taaaatctcc gataaagtgt tagaagaaca actagctatc gggcgtacac cgattcaaga
    13441 agcactgatc caactgcgca atcaagagct tgtaaaaacc attccacagt ccggcactta
    13501 tgtttcaaaa atcgatatcc gatctgcttc attagcccgt tatacgcggg aaaagttgga
    13561 aaaaccgatc ctgcaagaat gcagtgcgaa aatgaccaag caagatcaag ctaaactaga
    13621 aaaaatacta gaacaaaccg atcaggcagt aatcgctcaa aataaaaaag ctttttttga
    13681 tttggatact gattttcacc gaacttgtta tgaaattgct ggaaaacgag aaatttggga
    13741 ttggctagaa agttatagta cgcacttgaa ccgtttcaga tggttacgtt tgacgatttc
    13801 agaattagac tggggtcgag tattagatga acatcagaca atgcttcaat caatgattga
    13861 tcataacttt gatgaagtcg gatttctttg cacgatgcat ttacatatga ttatcgagga
    13921 gcaagaatac gtgattcata attaccccga ttattttgaa gatattcagg acagacccat
    13981 caaataagag gttttggaat cagcgttttg acctgagtat tagagaaaga aacggcaaaa
    14041 tagttcctca tattttgccg ttttttttga gctaataccg agggtcagct gattgcacac
    14101 cgtttattga gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
    14161 aaatagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    14221 caccgttttc caagaggtcg tgagcctgcc gttcatctcc gagtcacaag gaacaatttc
    14281 aaaaaacagc ttctcatgtt tgttgaatat tttgacttgt gaccgaggag ttgacaggag
    14341 aacatcgttt attcggaaaa aaaggactgt gacacgactt ttgtcacagt ctctttattt
    14401 gtaatctaaa tcatactccg tacatctttt ttctattttt tttatttctt ctaagatccc
    14461 ttggctacga atttcattat atggccaaag taagactgcc tctaatacat aaggcgaaaa
    14521 atcgctttga ttcatctctt ttgaaaaact catcgctgct atctcgctca atgcacgaat
    14581 gtttgattga tgtgtataaa aaggcaaccg ccacagcaca attttttcgc tttgcgtata
    14641 aattccctta acagaagctt cgatgtggtg aaatagttca tgtccaataa tcaaagcttg
    14701 cgctgtttct ttagagatcg aagctagtcc tgaacatttt aatttttcta acggctccgt
    14761 catcaatcga atcccttcat cttctgcata agttgcaaaa gtgattcgtt cgtttatttg
    14821 ctctgattca caggaaacga ttggaacagc catttgttca caaatttctc ctacatcggc
    14881 aaaagggtac ttttttttga tgctacatgc cgtttcgata ccacaatcga ttgattgttg
    14941 tataagaatt ttctgcttgg acaaaggaat ctttttccaa aataaatctc gccgaaactg
    15001 gtagaatccc caaatttctt cggggatctg ttgtaattcg tttagtaact cttctaactt
    15061 atgtttgttt gatatttccg gcaacgacga tcacttcttc ctccggttcc atagtagata
    15121 cttccagatc catcaaaagc tgtagttgtt ccagatccgt cgctgtaaaa tcagagactc
    15181 ttgctcctaa gcagagataa aattctggtc gggcaatcaa ttcagtttga taaactgcca
    15241 tatcttccca aagttgttcg acagctgtca tataattggc tgcagttgtt ttcatacatg
    15301 aagcatgtcc acgttggacc ttgataaatc ctttttgatc gatgatccga agacttccag
    15361 cattttttga acgattctgt tcgccgcata cataaaagaa aggtgtattt tccaataagc
    15421 aaacttcatt tttatttaat cgcatatcct ctgcagcaag ctctagtgct tcttctgttg
    15481 tgatctcctt ggttagatcg gttgctttca cttccgtaga accagtagcg atagccgtta
    15541 cttttgaagt ctgtggatcg atttctacgt ggacttcgat cgattctggt gtagcgcctg
    15601 attcaatcgc tttattcatc gcttcatttt tcaacgatcg tatatcttct ttacttggcg
    15661 aagggatgat acgttctaca acatctcgga ccattgcaag tgctacacca atagaagaaa
    15721 tcacttcggc gttttcagga atcgaatatt tcacacccat tttattacta aagtaagtaa
    15781 tcaatgaggc agcgccgccc cctacaccta caagagaaat ctgatctttc tccaagtgat
    15841 acttgtctgc caattctaaa atcaccggtt caatcttagc ataggatttt tccatgatct
    15901 gttctgcgat gtcttcaacc gtcgtatgac aataatcagc taatgcttgg atggcttttc
    15961 gtgcagatgg gacatttcca taagaaaaat gttcttcttg cactaatcca aggacattgg
    16021 ctgcacatgt atttgtaata gtcacttctt ctccgtcttc catcactacc ttgacgtaat
    16081 cagcagggtc tccttcttta ggagagaaaa attcaacctt aggacctttg attttttctg
    16141 tctccgtaaa tactgcataa tctagaccgg caatatgtgc agaacgtgga ccgacatcga
    16201 tgattcctga ctgattagcc cgtaccatcg aaccgcctgc cacacctaga acccgaacat
    16261 ctaatgaaga gatatatgtt gggtggccac ctacaattga ataatcgatt gctggacgtc
    16321 catttttaat gaccccgata ttggttgttg ttccgcctac ttcaaaataa acaccattag
    16381 acgctctcaa gtacatcaaa gagcccatga ctgatgctgc tggaccggaa agcatggtta
    16441 aaacaggtcg ttttttcatt tcattgattt ccattactcc gccgtctccg cgcataatca
    16501 tcaaagaaac attgacccct gcttctcgta cggaatcttc tgtagaggtt gccgtatcta
    16561 gcatttttgg taaaatagat gcattgattg ctgctgtccg agttcttctt gtcaatccat
    16621 ataattttgt gatgtcagaa gccatcgttg tagggatcga ttttaccgat gctgcttcat
    16681 aaacaactcg ttctggttcc ccattatcta ctccaaatgc catagaagaa accaagacct
    16741 ccgctctttc tctttctaaa gaagaaatcg tttctgaaac acggtctaca tttaagtgtt
    16801 tgacaggtaa gaatgcattg acaatctcta tttttttctt attccctaaa tcgatatcgt
    16861 tcaatctagt ttgtcgtttt gctaaaaaac cttctaatcc accttttgcc atcccgatta
    16921 cgccgacttt tgctacgtct ccttcaatca gtgcattagt cgcttgtgtg gtagaatgtg
    16981 caacaaacac aacatcttct ggactgatat tattttccag caaacaattt tgaaaagact
    17041 gaacgacacc tgcggctact ccacggacat catcgtgggt tgttttcact gaagattttc
    17101 cgatgatttc gtgcgtcgca ttatcgattg cgactgcttt cgtatgtgta ccgccaacat
    17161 caattcccat tcgcacaaaa cgtttcattt tcttttcctc cattcatcta atcgttttta
    17221 tcgacgtttt gtttactagg cgttcccgta caataagaac gtgatcaggc ataagatcgc
    17281 tccgatgatc caaccagttg ggatggattt cttcatatat tctcgagaag ttacttttgt
    17341 ataacctaat ccccaagcaa cccaagattg tgtaatatct aaatgctgag gagcaatcgt
    17401 tgtgattgca aataacggga acaaaaaggc tggcgacatc gttggattaa cagctaatac
    17461 gacagctaaa atagctgagc ctgaaccgac tagattcatt ggaccgcgga aaaatcctag
    17521 tggtgtcaat atagcaaata cgattgccaa gactaatggt gattgcggta tccaatctcc
    17581 aaaaatagct gaaaaataag gagaagcata cgcagctgaa ttattgaaca tcgctaaggt
    17641 catcaagaaa ccaaccattg gcgcaacatc tactgcgcca tctgtaaata atttcgcaaa
    17701 ctgacgacag atttctttat aacctccgcg caattttcca gtaaacagca atgcccagat
    17761 acctgctaga ataaaaccta agatgattgg aagatctaaa agaacgaccc ctaaaactgg
    17821 taataatacg gctaaccatg agatcattgg tgcgtcgcca ccttcagctg gtacgtttgc
    17881 tgccattgca tagcgtttct ttttgttcat tgatatatta gcgaccgtta gtaccacaac
    17941 taagcttaca atcatcccaa tcataccaat ttggaaatag tcattataag tatagctttc
    18001 agccgctgga ttgaaaccag cataaatcgt ttgatattgt ttgaaattga cgatattagc
    18061 aaagattcca gccataatcg agcccataaa tgagaacagt gcaaccggta ctgaaatacc
    18121 taatgacatc aagattggca acacaataac tgcaatagaa atcactggtc cgattcctgt
    18181 catcgacatg aatataacag cggttacaat acatagtaag ctcatcgtga ttcttggctt
    18241 gtcccctcca agttcaacta cctttcgaat cagggtagct gcgattccgc tatctactag
    18301 aacacgcccg aaaaaggcac caaagaagac atttaccaag atcgatttgg cgtactcagc
    18361 tggccccgtc gtatagatat gcgtcaacac atcaattact ccttgccctt ccatcgccgg
    18421 gtttggtgaa aacgtgtttc cgactagcgc taatcccatc catacagtag ccatgatggc
    18481 aaagccgacc atcaagttgt agccttttac acagtaccag accatgaaaa agaatgagat
    18541 aactaacaaa ataccaataa taatatcact catgttattc ctccgtccat tttgtttttc
    18601 tgataagcaa actaatatat cagtcaaact cattctaact tatgtagtta gcgctttcat
    18661 taaagggtga ccataaatgt tcagttttca attaaaaaac cttaattatg ctattttaga
    18721 cttatacgtt attgtcctgt ttcatttgat ggaggattgt aaataaaaaa agaggaaaga
    18781 acataacttt tcgttatctt ctttcctctt tataattgtt tagcttattt agccaaagct
    18841 tcttttgctt ggtcagccaa tgcagtaaat gctgctgcat cgttaacagc caagtcagct
    18901 aacattttac ggttgatgtc gatttcagca actttcaaac cgtgcattaa ttttgagtag
    18961 ctcaagccat tcatacgagc tgctgcgttg atacgagcaa tccacaattt acggaagtcg
    19021 cgtttctttt gacgacgatc gcgatatgcg tagttgtatg aattcattac ttgttctttt
    19081 gctgttttaa ataaagtgtg ttttgaaccg tagtaacctt ttgctaattt tagcactctt
    19141 ttacgacgtt tacgagttac tgttccacct ttaacacgtg ccatgtataa ttcctcctaa
    19201 taaaaataaa tcttcagatt ctgttatttg tacttcttat ttcatttttg ctagttgttg
    19261 gcgaatacgt ttgaagtcgc cactagaaac catgcttgct ttacgcaatt gacggcgttg
    19321 tttttttgtt ttgccgtgga aacggtgact tgtaaacgcg cggaagcgtt tcaatccgcc
    19381 tttaccagta cgtttgaaac gttttgctga tccgcggtgt gttttttgtt ttggcatgac
    19441 tatatttcct cctcaaaatc tttcgttatc cgactatcgg ctgtccaaca gccagttagt
    19501 cattcaaatt acttactgtc gtttttcggt gccagcgtca agaacatgct gcgtccgtcc
    19561 attttcgctt tttgttccac tgttgcaata tccgcagttt cttcagctaa gcgattcaag
    19621 actttttgac caatttcttt atgggtaatg gcacggcctt tgaaacggat agaagctttc
    19681 actttatcgc ccttttccaa gaacttacgt gcattacgaa gttttgtatt gaagtcattc
    19741 aagtcgatcg taggacttaa gcgaacttct tttacattga tcactttttg ttttttacgc
    19801 gcttcgcggt ctttcttttg ttgttcgaaa cggtatttac cgtagtccat gattcgcgca
    19861 actggtggtt tcgcaccagg ggcaactaac acaagatcca agtttgcttg ttctgcaata
    19921 ttcaatgctt ctgcttttgt cttaactcct aattgctcac catctgaacc gatcaatcgt
    19981 aactctcgtg cacgaatgcc gtcgttaacc atcatatcct ttgctatggt cattcacctc
    20041 caaatattat gagagaaaga aaagcttacc gaataaacgg caataaaaaa acgagttctc
    20101 ttacagaacc cgcgcgtact tcacctaata gaaccagtga aaactatcta gcccagtgac
    20161 attatcaact aaggcgagaa gcgggtgctt ctgcttttat ttctcaacca tcatagtata
    20221 gcaaaaacta atctttttgt caatgttttt catacagttt gtcttccttt tctgaaaatg
    20281 attagattag cagactttct tgcagtgatt cccgcttctt tgctatacta aagaaaaacg
    20341 aggtgaaccc acatgaaaaa aatcgaacct attccacctt ctcttcctct tcttacaaag
    20401 ggagagtttt atttagaaga tgaggagatc ttctctggtc ttctgatcaa cgggggtgac
    20461 caaagctatc tcgatgccaa aaatatggta ttaagagaga gtcatatcca aaaacttacg
    20521 atgcaaaaaa cagttttacg tcattttgaa tgcagtaatg tcatttttga aaaatgtgat
    20581 ttttccaatc tggagtggat tggtggcagt ttccaccaag tcgtgtttca tcaatgtaaa
    20641 ttaacaggta cgaattttgc cgaaagttat cttcgagact gtactttcac tgattgtatt
    20701 gcttcgatgg cttcctttag tagtacaaac ttgaaagctg tctccttcga tcattgtcaa
    20761 ttagaagaca gcgaatttta tgaggtcact tggaaaaacc tttttttgtc tgacaatcag
    20821 ttgaccggtt cgaactggtt tcgaacttct ctgaagggat tagactttac gactaatttc
    20881 ttcacagcaa tcgctttatc tcaagattct ttggcgggac taatcgtcaa ccaagagcaa
    20941 gcacttgtga tcgcccaagc ccttggtttg cagatcaaag actagatcaa cgaatccgcc
    21001 gccacagtgc aataagcatc caaatagata agaccaaact gatgatcaag gcgattcccc
    21061 atccgaaaac ggaatgggta aagggtaact ggagattcat tccgaagaat cctgtcacaa
    21121 tagttggaac ggtcaagatc agcgaccaga ccgtcaaaaa tttcatcgtg tcattcaagt
    21181 tgttattcag caaattatta taagtgccag agagctggtc taaaatctgc gaagctaagt
    21241 tcgtcatttc aactgcttgt cgtgcttcga tcagcgcatc gtccaactct tctttttcct
    21301 tttctgacaa cttgcgataa atagccaatg ccttgatttg ttctaaaagt actacatttt
    21361 gttttgttcc tgtaactaag taaaccaaac cgatctctaa atctgaaagt tgcagcagat
    21421 tcttgttcgt tgttttttct cttaatttca gattaagccg ctgacgttca ctattcactt
    21481 cctcgattaa aggaaaaaag gaatcagaaa tcaaaaacaa tgtacgaaac aataaactgt
    21541 atacactcat gtctggcatt cgttccaaaa gcgatcgaat tagtcgaatc acgtactcgg
    21601 tattcggcgt ggtaaatgta aacaaaccgt cttcttttaa aataaaagcg ataggactag
    21661 tttcataatg attatcgact ttttgctggt gcggaacatt tgatacaagc agtaacgctt
    21721 catcaaaaga atcgtactct acgcgcgccc gttcattttt atctagagaa taagtcagca
    21781 tctcgtcact aatgccatat tcttctttta agtttttaac agctagcaca tcgttttgct
    21841 gtacattgat ccaaaaatta cggtcttgat taaaatagtg tttttcccgc atactctatc
    21901 ctcctgacat ttgtttccag tataaagaaa aaagtcgctc aaataaaggt ttacccttta
    21961 tttgagcaac tgtaaatcaa atggctactt tggtttggcg aatctctgct aatttttctt
    22021 cgatcatttt caagacttct tcttttgctt gaggatcttc cacaaaatta tagcggtctc
    22081 catcgatttg gatttttgga ctttctttgt attcttcata ccattgatcg taacgtctgt
    22141 tcaattcttt gtaataatcg tatagacttg gatcaaagga caattgttca tacgaacgac
    22201 cacgtttttc gatacgctcc aacatcgtat caaaagatac acgaatatga actaataaat
    22261 ccggatgttt tttatgggca gcatagggaa gttcttccat catgttttcc agcagttcat
    22321 catacacacg aacttctgtt tctgttgctc ttcctagatc tgcatttaga tggaaaagca
    22381 gtgaatcttc ataaatcgaa cggtccaata cattattgtc atctttcatt gcttgtttga
    22441 tgcttgcaaa acgtttattc aaaaaataaa tttgcagcaa aaaagcatac tgttcagggt
    22501 ttgcataaaa taagggcaag acttcattat catcaatcga ttcataaaat gcctgactat
    22561 caaagtgttc agccatcatt tctgttaaac ttgattttcc ggcaccaatt gtacctgcta
    22621 aaacgatcac gctcatttac ctccagctca cttcatcact aaatattgtg tttaactcca
    22681 acctttttaa atataccccc aatatctagt agtgtcaatt gtattttttt ctagtcaaag
    22741 aagaaaaaaa gtcaaaaaat tcttaagaaa aaagtgtttt ttctttagaa aacgtattct
    22801 tagttttact tctgagatgg ttttagtaaa ataaaggagg aatgtttttt gagaccctta
    22861 gtggacaaaa aaattcgtga agcagcgcaa ctcgttttgg gcgctttatt ttttgcttaa
    22921 aaatcacttt tacgtgagaa ggagttagat tatggcaaac tatggccaag gagaatcaag
    22981 cggaaagatc atattgatgg gcgagcacgc ggttgtttat ggagaaccag cgattgcctt
    23041 tcctttctat gcaacaaaag tcaccgcatt ccttgaagag ctggatgcaa tggacgatca
    23101 actggtttct tcctactatt caggaaattt agccgaagct cctcatgcat taaaaaatat
    23161 caaaaaatta ttcattcact taaaaaaaca gcatgacatc caaaaaaact tgcaactgac
    23221 cattgaaagc acgattcctg ctgaacgtgg aatgggatca agcgctgcag tcgccacagc
    23281 agtcactcgt gctttttatg attacttagc atttcctttg tctcgtgaaa tactattaga
    23341 aaatgtccag ctttcggaaa aaatcgccca tggtaatcct agtggaatcg atgcagccgc
    23401 tactagcagc ttgcagccga tttattttac aaaagggcat cctttcgact acttttcttt
    23461 gaacatcgat gcttttttga ttgtcgctga tacaggaatc aaaggacaaa caagagaagc
    23521 cgtcaaagat gtcgctcacc tctttgaaac ccagcctcat gaaactggac aaatgatcca
    23581 aaaattagga tacttgacga agcaagcaaa acaagcaatc attgaaaatt caccagaaac
    23641 gttggcacag acaatggatg aatcacaatc acttctggaa aagctgacaa tcagcaatga
    23701 ttttcttaat ctattgatcc aaacagcaaa agataccggg gcattgggtg ccaaattaac
    23761 tggcggtgga cgcggtggct gtatgattgc attagcacaa acaaaaacaa aagcccaaga
    23821 aatcagtcaa gcacttgaag atgcgggtgc tgctgaaact tggatacaag gattaggagt
    23881 acatacctat gtttaaaggc aaagcacgcg catataccaa cattgctcta atcaaatatt
    23941 ggggtaagaa aaatgaagaa ctcatccttc caatgaacaa cagcctttcg ttaacattgg
    24001 atgcatttta tacagagaca gaagtcatct tttctgatag ctatatggta gatgaatttt
    24061 atctagatgg caccttgcaa gacgaaaaag caacaaaaaa agtcagtcag tttcttgacc
    24121 tttttcgtaa agaagccggc ctctcactaa aagcttcagt aatcagtcag aatttcgttc
    24181 ctaccgcagc tggattagcc tcctctgcca gcgggctagc tgctttagca ggcgcttgca
    24241 atactgctct taagcttgga ttagatgatc tctctctttc aagatttgct cgacgcgggt
    24301 ctggttcagc ttgccgaagt attttcggtg gtttcgtcga atgggaaaaa ggccatgacg
    24361 acttaagttc ttacgctaag ccagtccctt ccgattcttt cgaagacgat ttagcaatgg
    24421 ttttcgtttt gatcaacgac cagaaaaaag aagtgtccag cagaaatggg atgcgtcgga
    24481 cagtcgaaac atccagtttt tatcaaggct ggttagattc cgttgaaggg gatctatatc
    24541 aattgaaaca agcaatcaaa acaaaagatt tccaacttct cggagaaacg atggaaagaa
    24601 acggactaaa aatgcatgga acgactttag ctgcccagcc tccattcact tactggtctc
    24661 ctgacagctt aaaagcgatg gatgcggtgc gacaattgag aaagcaaggc attccatgct
    24721 actttacgat ggatgctggc cctaatgtca aagttttagt ggaaaacagc catttatctg
    24781 aggtacaaga aacatttacc aaattattta gcaaagaaca agtaattact gcacatgctg
    24841 gtccaggaat tgcgattatt gaataaaggg agatttttat gattgaagta tctgcaccag
    24901 gcaaactcta tattgccgga gaatatgcag ttgttgaaac aggccatcca gcagttatcg
    24961 ctgcagtcga tcaattcgtg acagtcactg tagaatctgc acgaaaagtc ggaagtatcc
    25021 aatctgcaca atatagtggg atgcctgtac gttggacaag acgcaacgga gaattagttt
    25081 tagatattcg ggaaaatcct tttcattata tccttgctgc tattcgcttg actgaaaagt
    25141 atgcacaaga aaaaaacatt cttttatcct tttatgatct gaaagtaaca agtgagttag
    25201 acagttcaaa tggccggaaa tatggattgg gttctagcgg tgccgtgacc gttgcaacgg
    25261 tcaaagcctt gaatgttttt tacgcgttga atttatctca gttggagatt ttcaagattg
    25321 cagcactagc caatttagca gttcaagata atggttcctg cggcgacatc gctgctagct
    25381 gttatggtgg ctggatcgct ttctcaacct tcgaccatcc ttggctccaa gaacaagaaa
    25441 ctcagcattc tatcagtgag ttacttgccc tggattggcc aggtctatcc attgagccat
    25501 tgattgctcc tgaagattta cgtttattga ttggttggac gggtagccct gcctctactt
    25561 ctgatttggt cgatcaagtt caccgttcga gagaagataa aatggtggct tatcagcttt
    25621 tcttaaaaaa tagtacagaa tgtgtcaacg aaatgatcaa agggtttaaa gaaaataatg
    25681 taacgttgat ccaacagatg attcgaaaaa accgacaatt actgcatgat ttatctgcaa
    25741 tcactggggt cgtcatcgaa acgcctgctt tgaacaaatt gtgtaattta gctgaacagt
    25801 atgaaggagc cgcaaaatct tctggtgcag gtgggggcga ttgcggaatc gtaattgttg
    25861 accagaaatc tggcattctt cctttaatga gtgcatggga aaaagcagaa atcactccac
    25921 tgccgttaca tgtctatagc gatcaaagaa aggaaaaccg atgaatcgaa aagacgaaca
    25981 tgtttcacta gctaaagcct ttcatgataa acaaaaaaac gaatttgact tcgtacggat
    26041 catccataac cctttgccgc aaatagcagt ttctgatgtc gatctaagta cacaggcagt
    26101 tgggtttaca ctaagcagcc cattttatat caatgcgatg actggcggaa gcgaaaaaac
    26161 aaaaaaaatc aaccaagatc ttgcaatcgt cgcaagagaa gcagatttga tgattgccac
    26221 tggttcagtc agcgctgcct tgaaagtccc ttctttagcc gatacttata cgattatgcg
    26281 acaagaatat cctcacggga aaatcattgc caacatcggt gcagggactt ctgtcgaaag
    26341 agccaaagaa gctatccgat tgtttcacgc agatgcctta cagattcatt tgaatgcacc
    26401 tcaagagttg gttatgcctg agggagaccg tgattttacc aattggaaag tacttattca
    26461 agaaactcaa acagccattg acgtccccct catcgtaaaa gaagtcggtt ttggcatgac
    26521 aagagaaacg ctcaatgatc tagctgcttt aggagttcat acagttgata tcagcggtcg
    26581 aagcggaaca agcttcaccc agatcgaaaa cgcccgtcgc tccaagcggg aactgagtta
    26641 tttagctgac tgggggcaat cgacagtctc ttcattgctt gaagcaaatg aagcagacac
    26701 ttctatggaa atcctagcct ccggaggtat ccgcaatgcc tatgacatat ttaaagccct
    26761 ttgtctcggc gcaaatgccg tgggtacttc aggaaccgta ctgacgcatc tgatgaatca
    26821 cggcgtagaa gaaactatta tactgatgaa gcaatggcaa gaagagcttc gtctgcttta
    26881 tactatggtc ggcgcaacga ataccgcggc cctacaccaa caatctctga tcttttccgg
    26941 accagtgaag gattggtgcg aagcaagagg gatcgactta gtgaagtatg gaagacgcac
    27001 agaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtacaa
    27061 acggcggaag taaaagcaac tccttcggaa ataagctaaa attcacaaaa atttgaaaag
    27121 caattttcgt gaattccttc ttatttcttg gagttaaatg cttctgtccc agcctctttt
    27181 ttcgaataaa tgatgtttaa tcagctgtcc ttcggtattc gttcaatccg ctgatttcac
    27241 aacctctcca ctaaacagtg tactaaagct cttttaaggt tgttttccga tataggcaag
    27301 tatgcctccg tccacataca agacatggcc gttaacgaaa tcagatgcgc tagaagccaa
    27361 gaagacagat ggtccagcca aatcgattgg ctcgccccag cgtgctgctg gcgtacgtcc
    27421 aacgataaat tgatcgaatg gatgacgttc accgttttct tgtgtttcgc gtaatggagc
    27481 agtttgcggt gtagcaatat aacctggtcc aattccatta cattggatat tgtattgacc
    27541 gtattcagaa gcgatgtttt tcgtcagcat ttttaatccg ccttttgccg cagcataagc
    27601 actgaccgtt tcgcgtccta attcactcat catggagcag atattgatga ttttaccgcc
    27661 gcctttttcg atcatatccg gaataactgc ttttgaaacg atgaatgggg catttaaatc
    27721 cacgtcgatt acttggcgga agtctgccgc atccatttcg atcatcggtg tccgtttgat
    27781 gatccctgca ttgttgacta gaatatcaac tgaacctaca tcttctttga tctgttgaac
    27841 catcgcttga accgcttctt catcggtcac atcgcagaca taaccttttg catcgatgcc
    27901 tgcttcttta taatgcgcta taccttcatc aactgattct tgattcaagt tattaaatac
    27961 gatttttgct cctgctgtag ctaaagagcg tgcaatctca aatccaatac catagacagc
    28021 accggtgact aatgctactt ttccatctaa acgaaacata tccatactaa attccatcat
    28081 tttctctccc ttatgcttat tttaattgat ccatttgcac catatccatg tctgtatagg
    28141 tgatattttc tccgcacatt gcccagatga atgagtaatt gcttgtacct acacctgaat
    28201 ggatcgacca gcttggcgaa ataatggctt gttcattgtc tacaactaaa tgttttgttt
    28261 catctggttt gcccatcatg tggaacacac gtgtatcttc attggcataa tcaaagtaga
    28321 cataagcttc catacgacgt tcatgtgtat gacaaggcat cgtattccaa gcgcttcctg
    28381 gttctaaaat cgtataaccc atttgtaatt gacagctttc gcatacattt ggatggatgt
    28441 attggtagat tttacgttca ttcagtgtca ggccttcacc agtctccatt ggttttactt
    28501 ggtcgatact gatttttaca tttggatatt tatgatgagc tggaacagaa ctaatataga
    28561 atttagctgg gttttcagca tcttgagaag aaaatacaac atgtcgtgtt tcttttccga
    28621 tatagtatcc gtcttgtttt ttcatttctt ctttttgccc atcgatctcg atgaatcctg
    28681 gtccaccgat attgatcaca cctagttcac gacgctctaa gaagtaatca acacctagtt
    28741 ctttgtctaa tgtgatttct aattcttttt cagtaggtgt cactccacca aagatcattc
    28801 gatcgttgtg cgtataagtc aaactgattt tccctggttc aaataccttt tctactaaaa
    28861 attcttttct caattgttcc gttgaataat gacggatatc ttctggactg tgtgtataac
    28921 gtgtttccat ttcttgcatt ttcttctact ccttctatcg aatgattttt ccagagccgc
    28981 atgctaaaaa ttgtgcgact tcttcttgac taaattggtt gcagtcccca tggatcgtat
    29041 gcttcaaggc agaagctgcc gtagcaaaat caatgatttc ttgcggagac atattggata
    29101 aaatcccatg gagcacgcca cctgcaaaag catctccgcc acctactcga tccacgattg
    29161 gggtgattct atggacttgg gattccacat attcatggtt catccacaat gtccccttca
    29221 attcattttc acttgctgaa tgaacggtac gcttggtcga ataaaacacc tgaatattcg
    29281 ggaacttttc ctgcattttt tgataataat aaattagagg ttgttcttct gcttctggcg
    29341 ctttctcgat acctaacaaa taaagagcat ccatttttcc agctgagcag atatctacta
    29401 aaggtaaaag ctgatggatt acttcgctgg cttcttgctg tgtccataat tttccacgat
    29461 agttgatatc gaagctgatc aggcagccag ctttttttgc agcttctatc acctgtttcg
    29521 tcattttttg ccatttttta gacaaagctg gggtgattcc tgaaacatga aatatatcta
    29581 ttccttggaa taactcctct agcgaccatt ccaaatgatc catttcagca aaacttgatc
    29641 cggcacgatc atagatcact gaggcggcgc gttcgccact accagtttcc atataataag
    29701 tacctagtct tgatccacca gataatacct gtttcgtatc tactccataa cattgcaagt
    29761 gctttttgac tgcttttccc aatgcgttat caggtacttt tgtagcaaaa atcgcttcat
    29821 gtccgtaatt agctaaagaa attgctacgt ttgcttcccc tccgccataa tgggcagcga
    29881 atcgttcact tgaatcgata cgggttccct gctctgtgga caatcgcaac atgatttctc
    29941 cgagtgtcac tacttttccc ataagcagct acctcttgat ttctttcatt tttgctgcat
    30001 actgttgcgc gacttccgtt actttgtcaa aatctcctgt agctgcgggt gctaaaaggt
    30061 tcccacctac tccgactgtt acgacaccag catcgaacca ctctttcata ttgtccagac
    30121 tcacaccgcc agttggcatg atattgactt gaggcatcgg cgccttgaag gcagaaatga
    30181 tacttggtcc ataagcgctt ccaggaaata gtttgacgat atctaccccg cttttcagcg
    30241 ctgttttgat ctcggtaata gtcatacatc ctggtaagta aggaatttga tacagattgc
    30301 acatctctgc cgtttcttga tcaaaggaag ggctaacaat atactctgcg ccagctaaga
    30361 tagccaagcg agctgtgaca gcgtctaata ctgtgcctgc gccaatcact atttctggtt
    30421 ggtctttgta gaatgacaaa agttctttga ttacttgatc tgcttgaggg actgtaaaag
    30481 tcaactcaat accagttaga ccacctttga tgattgcgtg gcaagcattc aaagcttctt
    30541 ctttgctttt tccgcgtacc accgcaatca ctccggcgtt tttcaatcga gataaaatat
    30601 caattttttt catcttctcc acctatttcc tatttagaaa ttttatttta taacttggaa
    30661 atctataccc atattatacg gcagaaaaga aaaattgcaa acgctttttt cattattttt
    30721 gaattctttg ctcgatttcg ctttttgttt gcttgagggc actgattatt tgttcttttg
    30781 tctcatctgt taaacgatat ttcggcatac ttacactaaa tgcaccaatg atttgttcgt
    30841 ttttcacgat tgaggctcct atacaaaaaa tatcacgttc gatttcttca tcgtcaaatg
    30901 cgatgtgggt ttcttttacc gttttgattt cctttttcaa ccgtaatgca ttggtgatcg
    30961 tatattctgt gtaagggatc aacggatgct tttcaagata atcagtgtac tgctcttcac
    31021 tgaattctgc taatacggct ttgcccatcg cagaattgta taatggtcgg gtgattccga
    31081 tttttgaaga catgcggatc gtttgatgtt ttggatctag tttgttcacg tacaagatct
    31141 cattgttgtc caatacccct aagtggatag tctcatcgat cttttgctgc agctgttcta
    31201 aaaacggtaa agtcaactct accaaatcga tttgttcaat gtttttattc gcgtagcgaa
    31261 taagctttgc acctaaacga tagtttttct ctgaattttt atttacgtag ccgatcatca
    31321 acaaagtatc tagtattttc aagactgtag aagaggtcat tcccacaccc tttgcgatcg
    31381 tctgcaaaga gacatcttgg ttttccgcta aatagtctag tattttagag gctttgatca
    31441 atacagttcc ataaggcttt ttattttcca tataatattc tcctatttct ttataagaaa
    31501 aattatagcg gaaataccat caatttaaaa gagatatcca gatataaact accatttctc
    31561 tttacaggca atttaatgtt gaagatccgc ttcgttttta catcaaactt tgatataaag
    31621 caatgatttc ttctactgtc gtttctcttg gatttcctgg tgtgcataca tctcgcaaag
    31681 catcttctgc aattgctgga atatctgctt cttttacacc taattcagaa atggtttctg
    31741 ggattccaac cgctttgctc aaccgatcga tctcaccaca agccgcttct cttacatctt
    31801 ctaaagacat ttcagctgct cctttaatcc ctagaacaag agcgatctca cggtactttt
    31861 ctccagtata ttctttgtta tatttcatga cagttggcaa aagtgctgca cacgctacgc
    31921 catgaggaat gttgtaccat gcacttaacg ggtgagccat cccgtgtacc agtcctaaac
    31981 cgacatttga gaagcccatt cccgcaatat actgtcctaa tgccattttt tctcgcccat
    32041 tttgatcgcc atctacagaa gcttccaaag aatggccgat gatttcgatt gcttttaaat
    32101 gcagcatatc agtcatttcc caagcgcctt ttgtgataaa cccttcaata gcatgcgtca
    32161 tagcatccat acctgttgat gcagccaatt ttttcggcat tcccatcatc atatcgctgt
    32221 cgatgaacgc gacgattggg atatcatgag gatcaacaca aacaaatttt cggtgattta
    32281 cttcatctgt gatgacgtag ttgatcgtta cttctgctgc tgttccagaa gtcgttggta
    32341 tagctaatat aggtaaacat ggatttttcg tatcagcaac accttctaaa ctaatgacgt
    32401 ctgaaaactc tggattcgtc aaaataatcc caatggcttt tgccgtatcg attggcgaac
    32461 ctccaccgat tgcgatcaaa cagtctgcct ctgcctcttt aacaaaagcc actccatctt
    32521 ttacattttg gatcgttgga ttaggtacga tcccgtcgta taaagcataa gcgattgctg
    32581 ctttatctag aagttctgtt acttttgttg ctataccgtg ctcgatcaat cctttgtcag
    32641 taatgacagc tgctttgccg aatcctcgct gctgaaattc agggacgatc gactcaatcg
    32701 ctccttttcc aaaatacgat gtttcattta aaatcattcg attactcatt ttctattcct
    32761 cctaatttgt attatttttc acaataaaaa cgaaagaacc ccaagcaacc ggttgcccag
    32821 ttgcttgttg ctacagttca aagactggca tcaattccca actgactgga gaggcatctg
    32881 gatttgtctc catgatatct tccatgtact tccaccattt ttgattgata gatgtatttg
    32941 ccattttttc ccataacttt tcatcttcga tcactaagta accaaataac aaacttgtac
    33001 tcggatcaag atagatcgaa tagtcgatga ccccgtgttc acgaagtgct gtacgcattt
    33061 ctggccaaag ttcatcatgt ctttttttgt attcttcttg tttttctggg taaactttca
    33121 tgcaaaaagc ttttttcacc atgtcagaca cctgctttcg tatgaacttc ctccaaataa
    33181 cctgcttttg gagtgacacc aaacgcatct gctaaatgcc acaggttttc gtccgtgatt
    33241 gtttgcaaga ttggtccttg cgctttgacg tacgtataga cttctgctgc cttttctgct
    33301 gtctcgatca aaccaaagac ttcatccatg tcttttcctg acccgtagat tccatgttgt
    33361 ggccataaaa ccaatcgagt ttctttcatt ttttcagctg ttgcttcccc aatctcattt
    33421 gttcccggaa ccatccacgg aatgatggaa attccttctg gaaaaacaac taagcattcc
    33481 gtacacattt gccatagcgt acgactaaag gaacgttcgt ctaattcatg cgtaaaactc
    33541 atcgctaata gatgactagc atggcaatgc ataacgatcc gattatctgg gtctacttct
    33601 aaacgggcga tatgactcat gaaatgactt ggcaactcgc ttgtaggaag gcattcgttt
    33661 tccagccccc acaaaagttc cagtgttttg ccatcttcag caactcgaac gattccaaga
    33721 ttctcggcag gttcgctcgc tacattttta aaatatcttc ctgaaccagt tacaataaaa
    33781 tagcatcctg ctaaacggct ggcatcaaaa atcatcgaga tgcggcggat cactttttca
    33841 ggatccaaga acggtgtgat ctcttcttct tttagtaaat aagaaatatt cccgccattt
    33901 ctttcatccc atcccaagcg ataaagattc gacgtcgttt cgatcatttc tttcacataa
    33961 ggcgcttgta aaatatctat ttttttcaat atagccacac tcctattttc ttaattccaa
    34021 cacgtctttt tgatattcct ggacttcttc cagccaatcc aaaccaactg gaacttgatt
    34081 tttgtaacag aaataattcc atacatcggc atacgggaaa tctttcaatt cttccgtata
    34141 agccattctt gaagtaaagt caaaagccag ctccatttct ttcaatcgct caactggttc
    34201 cagcatagct ttcagcagtg ctttttgtgt atttctcgta ccgatgaccc aagcagcgat
    34261 acggttgatc gttgcatcaa agaaatccag accgatattt gttttttcca acaattcgtt
    34321 acggactaat tctttagcga tttcctgcaa ttcatcatcc atcaaaacca catggtctga
    34381 atcccaacgc actggacggg aaacatgcaa catgattcct ttgctgaata aagctaatga
    34441 agataattta ttggaaatca cttctgttgg atgaaaatgt ccagcatcta gacaaacaag
    34501 tttgtcacga gtcaacccgt aacccatata aaattcatgg gaaccgactg tataagcttc
    34561 agcgcctaaa ccaaatagtt tgctttctac tgcatcttgt acataccgtt catctaactc
    34621 ttctgtaaaa atttcatcga gtgattccat taaacgttcc cgtggggtaa aacgatcaat
    34681 cggattatct ttcatcccat caggtaccca aaagttattg atacttgttt gcccagtttc
    34741 tttcccaaag tactccgcga ttttgcggct tcttttcccg tgttcgatcc agtattcacg
    34801 aacttcttta tctggatgag ccaatgtaaa gccgtctttc atcattggat gcgaaaagaa
    34861 agtcggattg aagtcaagac cgatttcttc tttttttgcc cactctaccc atttttcaaa
    34921 atgtttcggc tcaatttcat ttagatcgac agattcttct gtatccaaat agatagcatg
    34981 aaggttcaat ttgtgtttgc ccggtatcaa cgaataagcc ttctccagat cttgtctaag
    35041 ctcttcaggt gttcgtgcgg ctcctggaaa attccctgtc gccatgattc caccggtcaa
    35101 ttcgccatcc ggtgtcaaga aacctttgac atcatctcct tgccagcaat gcatagaaat
    35161 tttgatgtcc tgtatctttt ctagtacagc atctgtatct acaccgatag ttgcatattt
    35221 ttcttttgct tgttcgtaat ttctttcaat cgtgttcata cgattactcc tttgatttta
    35281 ttcacgtacc attccatcgt gttttgctta ttttcatctg ggtaaaaagt ttcacatgga
    35341 aacgactgtt tgattacatt tcttgcttgt tctatattcg aaaactgacc tgtactgagc
    35401 atttgcatgg caacattccc gattgctgtg gcttcactag gtcctgcttc tacaatgatt
    35461 ccggcataat ctgctgtcaa ttgattcagt agacgattat tcgatccgcc ccctacgatg
    35521 tgcagctttt ccaatttcgt tccagttaag tgttctactt tgatcagctc ttgtgcgtaa
    35581 caaagagcaa gattatcata cacacaacga gcgatagctc ccgatgagac agggactggc
    35641 tgctctgttt caaaacagta ttgctgaatc tcttctatca tatttggggg attcaaaaat
    35701 cgagggtcat tgatttccat cacttgctgc agcggttttt cttttgcagc aagctctgcc
    35761 aattccgcat aactgtactc gtactcttgc attcgggcaa cttcctggat caaccacatc
    35821 cccatgatgt ttttcaaaaa acgcaccgtg ttatgggcac cccattcatt cgtataattt
    35881 tcagaaaaag cattgcgaga aacattggcg atctttgttt caatccctaa aagcgaccat
    35941 gtcccactgc tgatataacc ccaacgttct ccttgacctg gcgttcctaa tacagctgac
    36001 gccgtatcat gacttgctac tgtgataaag gtcacatctg gcaaatcata ttttgaaaat
    36061 tgttcttttt gcagatctcc tagtatcgtt cccggctctg tcaatggtgg aaataattcc
    36121 ttcgagacac cgattttttc aagaagttct atttcccatt cttttgtccc tgcattcagc
    36181 agctgcatag tagatgcatt agttttctcc gtcaccatgt taccagtaaa gacataccct
    36241 aaataatcag gtatcagtaa caatttatgt gcttttgcca aattttcttt ttcttcagca
    36301 aacaattgga acaaggtatt gaaaggctgg atctgaatcc ctgtctgctc atacaactta
    36361 tctagagaat agatttcaga aaactgttct actgctccat tggtacgctt gtcccgataa
    36421 gcaatcggat catttatccg atccccttgt tcatcaatca gacagtaatc gactgcccat
    36481 gtatcgatcc ccgcaaaaag gcgatcaatc cctgtctgct ttaatttttc aaatccaatc
    36541 aaaatctctt tgataagata atcgatcttc cagcgatcgt gaccgtcttt gttccgaaag
    36601 ccgtttttga accggtgtac ttcttccaag atcaaagaac cttttgcatc cctcgtactt
    36661 ttcattagtc gtccgcttga tgcaccgata tcgatggcaa caaatgttgg attcatttct
    36721 ccacctcttt tactttattg ttgattagca ctttattaat aaagcgttat catttattga
    36781 caatgctatc ataatacaga atcttatatt ttgcttcaca ctattttggt atataattgc
    36841 attatcttga caagttggga gtgtttacat ggattactgg gaaaaagcca accactcatg
    36901 gtcggaagac tcaaaaagag tgatcttgac gcctacacaa agaagcagga atttattttt
    36961 ttatatccaa gaaatcggtt acttcaaagc accaatccct tactatactg aacgagcaca
    37021 tttaccttct tacttgataa agtttacttt gtctggcaaa ggttcgctta catataatga
    37081 taatcagcaa cagctaaaag ctggagactt attttttatt gactgccaag aatatcagcg
    37141 ttatcaaacg atcagcgaag aaccatggga aatggattgg atccatttga atggtcagaa
    37201 cgtccaatca ttttatcagg agttcacaaa agacggccat aacaccttcc attcaacagg
    37261 agatccatca gaaaacaaga tccaccattt gatcacagaa ttgatccaca tgcaacaagg
    37321 cacgaatgcc cgtaccgatt tccagtcctc tgtagctatc cacgagttgc tgaatgaact
    37381 gattttgcaa aagtatcagc tagatttcga agaagaggat attcccgcct acgtttacaa
    37441 actgaagcaa tacttagacg aacatttcaa agaaacgatt acacttgatc aattagaaca
    37501 acaattctta ttgaataaat atcagctgag caaagatttt tccaaattta tcggttctcc
    37561 accaatcgat tatttgatca gcaaaaaact ttcttacgct aaagaccttc ttcggtatac
    37621 agaatttacg gttcgaatga tttccgaaga aatagggatt gagaattttg cttactttag
    37681 ccgcctgttc aaagcaaaaa caggactatc gccgagagac tttcgtaaat tcagctgatt
    37741 catataatat gtacgtcatt ttttcaaaat attttgaaat atatagataa aaataaaata
    37801 accgcagttt ctgcttctat gtaaacaaca gcagctactg cggttatttt ttagtgatca
    37861 ttctcattgt tcatcttcaa aatgttcata atgaaccaac tgtttctgtg tatcacgttt
    37921 caaaaattct ttaggcgtta tgccgaactc tctcttgaat gctttcagaa aatgcggata
    37981 agaagaaaaa ccacttttac tatatacagc actagagctg cggtattctc gcaacaagtg
    38041 cttggcatag atcagctttt tcttcattac atactgatga aacgtaaatc cagtgtactt
    38101 tttaaattct cgtgtgatat ggtatttgct gacaaaaaac tgcttttcca tttcctctaa
    38161 agaaagcgga tgagataaat tttttgagat atattgtaac atttgttcca cacgctcatt
    38221 aggaactaca gcattctgtt cttgcgcact ctcttctttt tgtacaactt gattgagaaa
    38281 aatcaagtat tctaccagag cttgttcata ttttatgtct tcaccgtaaa tttttttatc
    38341 aggaggatgg tcgataaatg ataatttttg cttcaatact tcctgttcta tttgcaatac
    38401 acgactcctc ttctctccca ttggttgaaa acaagccgat agattagacc atggcgtgga
    38461 acgactttct agaaaagaag gggtgacaaa gatatagaca cgctcgaata catctgaaga
    38521 ttggcggacg atacgatgca aatcattgga atggatgagc agcacacttc ctgctttgat
    38581 cgtaaattgt cgtccgttca aataaaaagt cgcttctccc tttaatgtag catggatctc
    38641 atagaaatca tgatgatgat agatcgtttt attttggctg acgacatctt tgacatgata
    38701 gatttcatac tgatcagaaa ccatcgtatt gacttgttca ccgctgattt tcttttggag
    38761 catgtgccgc acctcctttt gttcacttat gcacaatttt attataccac gttttttgta
    38821 atcaaaaaga gctgctttta tatactctta taaaaaagaa gagacgtggc atcttctagc
    38881 cccgccccct ccattctaaa tattagttaa ccaacgtctt ctttacatta ttcaagaaga
    38941 cttcttccat aacgaatccc atcatctttt gtttcttcaa gaaccacata caggtacgga
    39001 cgattcttca tgatgatttc tttgattttt gtgtactcga tcacaccgtc tcccaaattt
    39061 accggcacga ttttttcctg ttcgacacga aaatctttca aatgaaccgc acatatcttt
    39121 tcaccaaaac gttcaaaagc ttcttctacc aattggactt gttcttgata atcactactt
    39181 gtgatcaaat tggtcggatc taaaatgatt cctagatagt ccgaatctac tgcttggatc
    39241 agctgctcta cccttgctaa agaatacacc ggatgattca aacctggttc tatccccacc
    39301 atcatttggt atttttctcc agcttttacc aagcgacgga taacggacac agcttcagca
    39361 aatgcttcat ctgtgaaatt ttcttccgta tattggattt ccggcaaaac acatccagtt
    39421 tccgaagcaa ccatcgacgc accaaaggaa gaagcatagc gaagatactg ctcgaacttt
    39481 gtcagcaact cttgtcgtat cgttaaatct ggatgaatca tattgatata gcaacttaaa
    39541 attcctacgg tcacttcttg cttttctaaa actcgtttga cataatttcc catcccagaa
    39601 ttgatctcat ttgcgccaga aggtatttca ggaaacgaaa gacctaaggc gagctgcagt
    39661 gtatgaatcc cttgttcttt tgttttttct gcaagatctt ctatcgtttg gacttcggaa
    39721 agatcatgtc ctctaattgt aatgttcaac ataactattc tcctaactca gcatattggg
    39781 tagccacatg acgatttgcg ggaagtaagt aatcaaaaac aagacgacta agataacccc
    39841 ataaaaagga agaagcggtt tcagcattgc ctctgctttt acgcctccta cacttgcacc
    39901 gacatatagt cctgtaccta ctggcggcgt gatggagcct acacacagat tcatgattga
    39961 aaacaacccg taatgtacag gatcaacccc tacacttgtg acgattggta aaaatattgg
    40021 tgtaaaaatc aagatagcag gcgccacatc cataaacata cccaccagta ataacacaag
    40081 attgactaat aacagaataa taaatttatt atccgttacg cccataatca acgaactaat
    40141 tgcctgagga ataccggtaa acgccatggc gaaagacatc atggaagaag tagccaataa
    40201 gaacatgatc gtccctgaca ttgctactga ttgaaacaac atattaggta tatcttttag
    40261 ttttaccgtt ttatagtaaa acatagcaat caacagggag taaatgacag ctatcgctga
    40321 agcttctact gctgtaaata tccccgtcaa gataccaccg ataatgatga caatcaatag
    40381 aaggctagga atcgcctctt ttattatttt tccagcatga gccatctgtc ctttttcgac
    40441 aacagggtat ttattctttt tagcaacgat gaatgctact agcatgattc ctagcgccca
    40501 caaagcgcct acgagataac ctcccataaa caaagcagag atcgaagcac caccagcaac
    40561 aagtgaatac atgataaaag cagtacttgg aggaatgacc atacctgtcg gagctgacgc
    40621 gatattgacc gctgtcgcaa acttccggtc atatccttct tccacttgct gagggatcaa
    40681 cactccgcca atcgcggtag aagcggcaat ggccgagcta gagatggaac caaataaagc
    40741 attccctaac acattcgtat gagccaaagc ccccggcacg cgcccgacga acaacatcgc
    40801 aaaatccact agtttcttag ctatccctcc attattcatg ataatgcccg agagaacgaa
    40861 aaacggaata gcgatcagcg aaaaactatt gatacttccc accatttttt gagctgaact
    40921 aaaagcagaa acatcaaacg gtataactaa caataaagtc atcatcgatg agaatgcaat
    40981 acttatagca atcggcatcc ctaatacaag caacacaaca aatacaaaaa ataacactaa
    41041 tccggcttct attgccattt tattcctcca tttttatatt ttttcttgaa ttgctggctt
    41101 gttgcttaat gtatcgatta gattgatcag gctatagata atgatgagta cccccgatac
    41161 aggtactgac agataaacat atcccatagg aatacttaaa gaaggcgaga tctgccccat
    41221 tgtcaatgtg acagcattcc atccgccata caccatgatc atcgcagcaa ataccagaaa
    41281 taaactttga acaacaactt ctaaaatttt actttttttg ccagttaatt tttcacttaa
    41341 aaccgttacg gctaaatgtg ctttttgtcc cacaacatac gcggaagaca acatggcaaa
    41401 ccagacaaga ccgaatcgaa caagctcttc cgtgaccgta tttggatttc ctaaaaccgt
    41461 tctggaaaat acttgccata aaacaacagc gaccatccca acaagcaaaa cagctcccag
    41521 gatctctaat accttgttta aaatcatttt catcacagct acgctccttg ctcttggatc
    41581 ttttgataaa ggtctttgta ggtaggatcg tttttgaata tatcatgaag tggggcaact
    41641 gcctctcgaa acggtgtatt atctacatca ttgaattgga ctccatgttc ttcttgtgct
    41701 aaagcttttt cttcttcaat tgcttttgca aaagcttcaa tttcaaaaac cgttgattct
    41761 ttcgctgctt cttcaatggc tttttcctgc tcatcagtta gctgttcttt gatggcatca
    41821 ttcatgatga tgatatctgg cacacgtgta tgctcatcat aggaatagta tttagccaca
    41881 ccaccatgtc cagctgtata caagacaaat tcattgtttt ctgatccgtt gatcaaattg
    41941 gattgaagag acgtgtacac ttcatcactt cccataggta cgggcgaacc gcctaaaagc
    42001 tctaccattt tgatagcggt ttcgctttgc atcacacgga tcttctttcc cttcaaatct
    42061 tccggcgtat ggatcggacc gtctaccata taaaagctgc gctgaccaga atcataatag
    42121 gtcaatccgg taaatcctaa atttttggtc gattggtaga ttggctccat tatttcttca
    42181 ttatccatca ccttaaagaa atgttcttca tcattgaata gataaggaac tgaaaaaatc
    42241 gaataatctt tagagaaact ttccaacgct gaggcactta cttttgtgaa atctactgct
    42301 cctgactgtg tcaattcgat cagttcacgt tctccaccca gttgtccatc tgggaaatac
    42361 tggatctgta cggaaccatc tgttttttct tttactaaac gcccaaattc attcattgca
    42421 tcaattactg gctggctgtt gcttgcataa gcaaagcgca aggtaactat ctcatttaca
    42481 tctgcatctg cgacagttgc cacactagaa ttatttccgc agccgcttag acctagcaga
    42541 cctaggctaa gcgaacttgc tgcaaacagc agtgatgttt ttttcatatg ggtttccttt
    42601 cttttaacaa taagagacga gcaattctgt caaacaaagc accgttaacg actgtccata
    42661 aggcattgct gtgattccga tattccgata aaaatctaaa ctgtccccca ttcctgtccc
    42721 gattgaaacg ttttgaactt ctcctttttc atcgatttgt tcaagcaatc cttggatagc
    42781 acgataagcg acttctttat attcttgtgg taaataacgt ttatggaccg ctttcaaaat
    42841 accgtaagca aatcctgcag ttgcagaaga ttctacgtaa gaagaaggat catccaacaa
    42901 cgtatgccat aaaccgcttt cagactgata agtttttaat gcacgcactt gtgcttcaag
    42961 tgtttcgatc aataatttgc gcaatgcatc ttctttcgat aattctaaga tttcaatgat
    43021 ctctggaata gcaatcgtca accagcagtt tccacgagcc caaaatgctt cggcatagtt
    43081 atgatttcct tcaaacgtcc agccgtgata ccacaatcct gttcgtttat ccatcaagta
    43141 tttgatatgg atcataaact ggtaacgagc ttcttctaaa tattctgggc gattcaacag
    43201 tttcccgatt tttgccaaag gaagcactgt catcatcaat gtatcgtccc acaactgatt
    43261 cttattttcc ggtccatatg tggcatgctg taacccgtct tcatttgtcc gaggcatgtc
    43321 attcatgacc cattctgccc attgttctaa ataaggcaaa tagcgtgaat ctttcgtgtc
    43381 ttcatacaag taagccatag tcaatagcgg tgccattgta ttgacgtttt tgggcggagc
    43441 gccttcttcc atacgatcct caaaccattc attgaccact tgataagcgc gaggattttt
    43501 tgtcatctgg taatttttat aaataccata tagaccgaca ccttgtggcc aattccacac
    43561 tgtccagctt ttgtcatcta ctttcagtcc atcaaagtcc aacaaaaatt caccattatc
    43621 gtctttgatc gttgtcaggt tttcaacgag caggtcaatt ttttctttca ctaattcttt
    43681 aggtacaaaa atctcttctt taataatcat ctttacgcca cctttttaaa cgctttcatt
    43741 ttatatcctt aatatagcag taataccgtc cactataaat atcgcttttt gaacaaaaac
    43801 tgtctaaaat tgacctaaat gttaaataac aaaagtaact ttcagttact tggaaattaa
    43861 aaaaagaact tggagaaaca acgttttcca agttcttttt catctatttt cttatctttt
    43921 ttctacgctt tcttttgctt atccgttgta agcatcaatg atgatctgtt tcaattcaga
    43981 gatcaatggt tctttcggat tagctgtcgt acattggtct tcgtaagcta attcagccat
    44041 acggtctacg gttgtatcta gtgtttcttg tgtgactcct tgagctttta gactcatgtt
    44101 gatgccgaca gattttccta attcataaac agccgttgct aaagcttcga ctaattcctc
    44161 tgttgtttcg cctttcagac ctaagaattt tgcgatatct gcgtaatctg tatctgcacg
    44221 gaaatagtca tatttaggga acatcgcatg tttttgtgga tcttttgcat tatagcggat
    44281 aatatgcggt aaaagaatcg catttgtccg tccatgaggg atcccgtatt ccccgccgat
    44341 tttatgtgcg actgagtgac aaatacctag gaatgcgtta gcaaacgcca taccagccat
    44401 tgtagaagcg ttgtgcattt tttcacgtga ttccatatca ggtgttttta ctgatttttc
    44461 taaatagtca aagactaact tgattgcttg taggctcaat ccacgtgtat aatctgaagc
    44521 catgactgaa acataggatt cgattgcatg tgttaacaca tccatcccgg tatctgctgt
    44581 gacagatgct ggaactgaca tgacgaattg aggatcgacg attgcgacat ctggcgtcaa
    44641 tgcataatct gctaacggat atttcacatg tgtttcactg tcagtgatga ccgcaaatgg
    44701 cgttacttca gaaccagtcc ctgatgttgt cgggatacat acgaattgtg ttttttctgg
    44761 tttgtcgatc ttatatgtcc gtttacggat atccaagaat ttttgttttg caccaaagaa
    44821 agatgtgtct gggtgttcat agaacatcca cattccctta gctgcgtcca ttgcagaacc
    44881 gcctccaaga gcgattactg tatctggctt gaagtctgcc atcagtttcg ttcctgcgta
    44941 tacggtattt gtcgatgggt tcggttcaac atctgagaag acttcgatct tcacatcatt
    45001 tttacgtcgt gccaatactt cacgaactgt atcgcagtaa ccgaattgga ccataccagg
    45061 gtcacaaacg atcatgacac gttcaacgtt ttccattttc tctaagtaca atagagagtt
    45121 cttttcaaag aagatttttg gcggtaattt gaaccattgc atgttatttc tccgtttcgc
    45181 tacagttttg acattgatta agtttacagc agagacgtta cgagaaacag agttcttacc
    45241 ataagaaccg catcccaaag tcaatgacgg aatcatttcg ttatagatat caccgatgcc
    45301 gccttctgca gatggtgtat tcactaaaat acgacaagct ttcattcgaa gaccgaattt
    45361 gacatgcaac tcttcgtttt ctgaatggat aactgctgta tgtcctaatc ctccaagatt
    45421 caacatacct tcacacaaat caaacgcatg gtccgtgttg ttagctttga tcatggcaag
    45481 aactggagaa agtttttctc ttgatagcgg ataatctggg cctacgcctt ccaattcagc
    45541 aatcagcatt ttcgttcctt ttggtacact gattcctgcc aattttgcga tttccatcgc
    45601 agaatgtcca acgattgatg gattgaccgc atattttcct tcgttcatca ctgcatcttc
    45661 taatttttgc aattcatctg gttttacgat ataaacttgg tgtgcttgga attctgcttt
    45721 tactgctgca tagatttctt tatccacgat cacagcttgt tcagaagcac aaatcattcc
    45781 attatcaaat gttttcgaaa cgatcaaatc gttcactgca cgtttaattt ttgctgtttt
    45841 ttcaatataa gctggtacgt tacctggacc tacacctaaa gctggttttc ctgtagagta
    45901 agcagacttg accattccag ctccacctgt cgctaagacg atcgcgatac ctggatggtt
    45961 cattagcata gaagtcgctt cgatcgaagg atgttcgatc cattggatac aattttcagg
    46021 agctccggca gcgatagcag catcacgtac cactcgtgct gcttctgcag aagatttttg
    46081 ggcacttgga tggaaagcaa aaacgattgg atttcttgtt ttcaacgcga tcattgattt
    46141 gaaaatcgtt gtggaagtcg gattcgttgt tggagttact ccgcaaacca caccgactgg
    46201 ttcagcaatt tcaatcaacc ctttttggac atcttcattg atcacaccaa ctgttttatc
    46261 atgtttgata ttatgccaaa tatattcgga cgcatacata tttttgattg ctttatcttc
    46321 gtaaacaccg cggcctgttt cctctacagc catcttcgct aatggcatat gttgatccaa
    46381 agctgccatt gccatttcat gcacgatatg gtctactttt tcttgatcaa agctttccat
    46441 ttctttcaat gctgcatttg ctttttctgc caaatcatca atcattgctt gtacgtcaat
    46501 tgtcttcgtt ttttcttttt ctattacttt agccatcatt atcctcctaa tgggtcatgg
    46561 ttatttgtta aataattcac atgtatatca tacaatattt atttttctat gtaaacactt
    46621 ttttgtgatt tttttcacag ttatttttaa atataaaatt gctttattta caggtgtttg
    46681 tatagaaaaa tatataaagt tatttattat tatttatatg acacacccga ttattcaata
    46741 ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac
    46801 atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt
    46861 ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt
    46921 ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt
    46981 tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt
    47041 tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac
    47101 ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg
    47161 acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca
    47221 tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata
    47281 acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt
    47341 tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga
    47401 ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca
    47461 aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt
    47521 ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg
    47581 catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag
    47641 taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag
    47701 cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt
    47761 ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac
    47821 cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg
    47881 ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc
    47941 gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga
    48001 atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga
    48061 ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc
    48121 cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg
    48181 ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt
    48241 cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt
    48301 ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca
    48361 ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt
    48421 tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct
    48481 atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc
    48541 cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca
    48601 aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta
    48661 aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga
    48721 gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta
    48781 ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg
    48841 gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg
    48901 gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg
    48961 atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca
    49021 aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat
    49081 tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct
    49141 attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga
    49201 tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt
    49261 catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca
    49321 gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa
    49381 agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta
    49441 aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct
    49501 ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt
    49561 ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata
    49621 aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg
    49681 taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca
    49741 aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca
    49801 catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac
    49861 ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg
    49921 ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga
    49981 tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc
    50041 ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg
    50101 taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat
    50161 tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac
    50221 tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt
    50281 gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg
    50341 gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt
    50401 tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac
    50461 gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg
    50521 gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag
    50581 tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg
    50641 gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt
    50701 cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca
    50761 gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa
    50821 agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt
    50881 ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat
    50941 atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta
    51001 cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg
    51061 tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat
    51121 tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg
    51181 ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact
    51241 gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa
    51301 caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca
    51361 tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta
    51421 aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt
    51481 gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg
    51541 cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa
    51601 gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt
    51661 cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg
    51721 catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact accagttgct
    51781 ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat
    51841 cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt
    51901 tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa
    51961 atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca
    52021 agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc
    52081 cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt
    52141 ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt
    52201 aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc
    52261 aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg
    52321 caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga
    52381 cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca
    52441 cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc
    52501 acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg
    52561 aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt
    52621 tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat
    52681 ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt
    52741 tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat
    52801 aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg
    52861 catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta
    52921 aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc
    52981 caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg
    53041 ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat
    53101 ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat
    53161 gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat
    53221 gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg
    53281 taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt
    53341 gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca
    53401 atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt
    53461 gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc
    53521 caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg
    53581 ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag
    53641 gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt
    53701 catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat
    53761 gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat
    53821 cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt
    53881 ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa
    53941 aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt
    54001 cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta
    54061 taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat
    54121 ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat
    54181 gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc
    54241 agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat
    54301 gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg
    54361 gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg
    54421 ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc
    54481 agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg
    54541 tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt
    54601 tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca tcatgagtga
    54661 aggccgtgtg atcaatttga tacaccagaa aatattttac gtcatcctgc aaacgatttc
    54721 gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc gacaagagtc
    54781 ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt acaagaagca
    54841 atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa ttcgaatgta
    54901 ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt atccagtgtc
    54961 ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct tcgtgatacc
    55021 ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga acagaacaaa
    55081 gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt gatttgggga
    55141 gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc taaggaggtc
    55201 tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa aggttttgaa
    55261 catctttata tttcaggact tgctttgatc attggtatca tcattgctgt accgcttggt
    55321 gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac cagtgccttg
    55381 cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg cgtaggtaaa
    55441 ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg taatacgtac
    55501 attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat ggggatgact
    55561 gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat catggctggt
    55621 attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta tatcggcgct
    55681 ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga tctcattttg
    55741 gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct aggtctcttg
    55801 gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa cgtatactaa
    55861 aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc cctggtcttg
    55921 ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca gaagcacaga
    55981 tcttagcaag tctagttgcc ggaatggttt aacattatac agataaaaat accgcaatca
    56041 tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga gatgcttcaa
    56101 tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt cctcctgaaa
    56161 aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga tacggtcaaa
    56221 cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga aaagacaccg
    56281 cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat gaattagtag
    56341 ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc ttccaagaaa
    56401 cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt tatgacgcag
    56461 tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg atcgccagtt
    56521 atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat gctgctccag
    56581 ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta aataagttag
    56641 gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat aataatctag
    56701 tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt gaagggaagt
    56761 gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc aggaaaatgg
    56821 ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg gtgtcctctt
    56881 tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta aattagcaaa
    56941 ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca tgatctccat
    57001 tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat ttttgtattc
    57061 cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca aaaacatcct
    57121 tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg tagagttacc
    57181 actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg ccatcgggat
    57241 aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta tccgaggaac
    57301 caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg cattaatggc
    57361 gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag ccagccgtac
    57421 atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg gtcgcaaagg
    57481 cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc tccttatggg
    57541 caaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc atactgccaa
    57601 cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg cattttgcca
    57661 atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa tactcgtctg
    57721 cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc atttaagcct
    57781 tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact ggtgactgcc
    57841 gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc atccttggct
    57901 aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc ctaaaaccgg
    57961 tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga tgatccttca
    58021 ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa aagacattgg
    58081 cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc atggccgaaa
    58141 gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat gtcgctttgg
    58201 atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta tcttttccaa
    58261 aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga ttgtccattg
    58321 cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt gtcttcgcct
    58381 cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat gctccaattt
    58441 tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt caacattcca
    58501 ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat taaagaaatc
    58561 atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt tttggggaga
    58621 ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat gtgtccaggc
    58681 agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc gatgctgtct
    58741 attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa aagactgcgt
    58801 gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt tctttaaatt
    58861 cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga ctgtatagtt
    58921 actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact gtcgtctttt
    58981 tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt ttcttgaatc
    59041 gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc actaataatc
    59101 tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt tttacgagca
    59161 gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga tataattata
    59221 gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca gcaaactttt
    59281 cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc cacgctcact
    59341 tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca acgtatcatc
    59401 tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat acacgaaggt
    59461 tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg cacgaaagat
    59521 ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct caaaaatgat
    59581 tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc ttagaccgtt
    59641 gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa atggtcactt
    59701 gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga ggtaaaggaa
    59761 gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa ttatgatcaa
    59821 gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt tcatacgctg
    59881 ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca aaacagaccc
    59941 tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg tttatttctt
    60001 caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc gcaatcttaa
    60061 ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta caaattgagg
    60121 aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga tatctacata
    60181 acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa aacggacatc
    60241 tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat cttcgtctag
    60301 cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca acgcaccgat
    60361 atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat aatcatgtag
    60421 ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat catggatctt
    60481 ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt cccctaaccg
    60541 atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa tattggcaat
    60601 cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc ctgtttgaga
    60661 taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt ctgttgagcc
    60721 atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc cattggaata
    60781 gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt ttgccagatt
    60841 gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta aaatcagcgc
    60901 tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt taccaatttg
    60961 agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga taaatgtgat
    61021 ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag cggcattttt
    61081 tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc cagttaaaga
    61141 ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg gcacagccgc
    61201 tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat ataaaccaac
    61261 aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata ggaacgatgc
    61321 agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg tcacagaaat
    61381 agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag cacctgctga
    61441 gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg aaatcaagcc
    61501 agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat gaggataagc
    61561 atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa caaaagcggc
    61621 aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga caacgatttg
    61681 ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca aagcagcaaa
    61741 tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta ctaacaacct
    61801 ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag ttttatagtc
    61861 gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag tcaataaact
    61921 ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa cgctttaata
    61981 cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat aaaaggatag
    62041 aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg gaagagagat
    62101 gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt tgtcacaacc
    62161 tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc aaattaaaaa
    62221 cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag cttttttaca
    62281 acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa aaaacggcaa
    62341 aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac actgatttca
    62401 caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga atttcgtcat
    62461 tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa caaacaaacc
    62521 gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg cagattcccg
    62581 taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga agaatttccc
    62641 tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta agatgataat
    62701 agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga tcatcatgaa
    62761 cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga catctactcc
    62821 tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca ttgtcaaaaa
    62881 tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt ctttctttcc
    62941 taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc acagacatag
    63001 tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa gcgcattggt
    63061 ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta gtttcaataa
    63121 tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt ctgctgcttt
    63181 ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag cagcgttcac
    63241 agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg tgatttcatt
    63301 ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt tttgctgaat
    63361 gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga cgatggtatc
    63421 catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg caggcatttc
    63481 tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg ttgagctgag
    63541 aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag tcccaatcgt
    63601 atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg tattgatacc
    63661 agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca attcatcaat
    63721 agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt tttgcagatt
    63781 gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt tcgtatttcc
    63841 agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat tttgttgttg
    63901 agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt catcatcagt
    63961 cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg tcgttgttgc
    64021 cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg gtatcgcatc
    64081 tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct gattttctaa
    64141 atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc cttgttttgc
    64201 ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt cgatctctgc
    64261 tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa ggatgatttt
    64321 tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt cacccatctc
    64381 atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt ccttgatttc
    64441 aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg tgtccaaatt
    64501 ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt tcgtatcgat
    64561 gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt tgataaattc
    64621 atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg gtgcaatcgc
    64681 attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac cactagtaaa
    64741 actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat actttccgga
    64801 acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta attgtttatt
    64861 tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt gaaactctgc
    64921 tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat atggatccca
    64981 taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg cgatgatcag
    65041 aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata atcgaagcgt
    65101 atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat tactttacca
    65161 tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt ggacatatca
    65221 cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat tacagccttt
    65281 actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat ctattatacg
    65341 tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt ttcagctttt
    65401 ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt cccagcagga
    65461 ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc agacagattc
    65521 attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc agcaaaggct
    65581 tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc ctttttttgc
    65641 cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg tttcatgatc
    65701 tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag gtctgcctga
    65761 tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga agctcctaat
    65821 ttttttacct tttcctcatt tttactggat gcactggtta agattcggat tcccttttca
    65881 tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat cattacagta
    65941 tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat tcctggcgtg
    66001 accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac ttttgctgat
    66061 ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac tcgatcacct
    66121 acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc atcattacct
    66181 ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg caaggcaaca
    66241 tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga accgacttct
    66301 ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc gttaattgca
    66361 aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc ctttattttg
    66421 acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt gtcttttttc
    66481 gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg ctaataaaaa
    66541 ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca aaaaaacagc
    66601 aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa aaagattacg
    66661 aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat cgtgatttaa
    66721 tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata aaattccgtg
    66781 aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa tccaagtctg
    66841 gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa aaagatacgt
    66901 tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt tacgcacagc
    66961 tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt tagtaacagg
    67021 agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg ccataatggg
    67081 tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc tgatctgctc
    67141 caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc caatgtgagt
    67201 cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg tcgggctaaa
    67261 atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt gctccgatcc
    67321 cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag gatcctcctt
    67381 attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca tagtggatcg
    67441 tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt ttgccgataa
    67501 gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt tgatccatac
    67561 atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa ccctgcatct
    67621 ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct gcttcaggag
    67681 tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg acttgaatca
    67741 attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt gcgtgcccag
    67801 agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt gtttcatcat
    67861 gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg ggaagttttt
    67921 tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct gcctgatctg
    67981 ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct ttgatgaatg
    68041 cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc ccagtgtctg
    68101 cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca gcagctgctg
    68161 cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca gctacagtca
    68221 gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca gcttctctca
    68281 actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct gcaactgctg
    68341 cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact gcaaacaatt
    68401 cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata gcttttttat
    68461 gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct tctttctagt
    68521 cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg acccatacat
    68581 tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt aaaatctcga
    68641 catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa gcctctttgc
    68701 aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac tggaatagtt
    68761 tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg atcagccgag
    68821 tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc ttttctcttt
    68881 tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca caaataagtc
    68941 cacaaataac aaaa
//
LOCUS       DAZKCC010000005        61088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000005 DAZKCC010000000
VERSION     DAZKCC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.005"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..1423)
                     /gene="sufB"
                     /locus_tag="ACG8VH_000337"
     CDS             complement(32..1423)
                     /gene="sufB"
                     /locus_tag="ACG8VH_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9108928.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1428..1898)
                     /gene="sufU"
                     /locus_tag="ACG8VH_000338"
     CDS             complement(1428..1898)
                     /gene="sufU"
                     /locus_tag="ACG8VH_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP9108929.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1885..3120)
                     /locus_tag="ACG8VH_000339"
     CDS             complement(1885..3120)
                     /locus_tag="ACG8VH_000339"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9108930.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="ACG8VH_000340"
     CDS             complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="ACG8VH_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9108931.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="ACG8VH_000341"
     CDS             complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="ACG8VH_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9108932.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5386..6234)
                     /locus_tag="ACG8VH_000342"
     CDS             complement(5386..6234)
                     /locus_tag="ACG8VH_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9108933.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6258..6944)
                     /locus_tag="ACG8VH_000343"
     CDS             complement(6258..6944)
                     /locus_tag="ACG8VH_000343"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9108934.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6937..7971)
                     /locus_tag="ACG8VH_000344"
     CDS             complement(6937..7971)
                     /locus_tag="ACG8VH_000344"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9108935.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8034..8290)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8366..8704)
                     /locus_tag="ACG8VH_000345"
     CDS             complement(8366..8704)
                     /locus_tag="ACG8VH_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP9108936.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8706..9062)
                     /locus_tag="ACG8VH_000346"
     CDS             complement(8706..9062)
                     /locus_tag="ACG8VH_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP9108937.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9068..10255)
                     /locus_tag="ACG8VH_000347"
     CDS             complement(9068..10255)
                     /locus_tag="ACG8VH_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9108938.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10446..11291)
                     /locus_tag="ACG8VH_000348"
     CDS             complement(10446..11291)
                     /locus_tag="ACG8VH_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9108939.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11525..12490
                     /locus_tag="ACG8VH_000349"
     CDS             11525..12490
                     /locus_tag="ACG8VH_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP9108940.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12867..13910
                     /locus_tag="ACG8VH_000350"
     CDS             12867..13910
                     /locus_tag="ACG8VH_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP9108941.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13972..14436)
                     /locus_tag="ACG8VH_000351"
     CDS             complement(13972..14436)
                     /locus_tag="ACG8VH_000351"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9108942.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14682..15629
                     /locus_tag="ACG8VH_000352"
     CDS             14682..15629
                     /locus_tag="ACG8VH_000352"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9108943.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15840..17912)
                     /locus_tag="ACG8VH_000353"
     CDS             complement(15840..17912)
                     /locus_tag="ACG8VH_000353"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9108944.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17845..18222)
                     /locus_tag="ACG8VH_000354"
     CDS             complement(17845..18222)
                     /locus_tag="ACG8VH_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP9108945.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18351..18911)
                     /locus_tag="ACG8VH_000355"
     CDS             complement(18351..18911)
                     /locus_tag="ACG8VH_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9108946.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19061..20353
                     /locus_tag="ACG8VH_000356"
     CDS             19061..20353
                     /locus_tag="ACG8VH_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9108947.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20413..21531)
                     /locus_tag="ACG8VH_000357"
     CDS             complement(20413..21531)
                     /locus_tag="ACG8VH_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP9108948.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="ACG8VH_000358"
     CDS             complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="ACG8VH_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP9108949.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="ACG8VH_000359"
     CDS             complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="ACG8VH_000359"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9108950.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24737..25336
                     /locus_tag="ACG8VH_000360"
     CDS             24737..25336
                     /locus_tag="ACG8VH_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9108951.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="ACG8VH_000361"
     CDS             complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="ACG8VH_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFP9108952.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27031..27375)
                     /locus_tag="ACG8VH_000362"
     CDS             complement(27031..27375)
                     /locus_tag="ACG8VH_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP9108953.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27512..28657)
                     /locus_tag="ACG8VH_000363"
     CDS             complement(27512..28657)
                     /locus_tag="ACG8VH_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9108954.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28837..29811)
                     /locus_tag="ACG8VH_000364"
     CDS             complement(28837..29811)
                     /locus_tag="ACG8VH_000364"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9108955.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29891..30766)
                     /locus_tag="ACG8VH_000365"
     CDS             complement(29891..30766)
                     /locus_tag="ACG8VH_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP9108956.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30896..32803
                     /locus_tag="ACG8VH_000366"
     CDS             30896..32803
                     /locus_tag="ACG8VH_000366"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP9108957.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33344..36622)
                     /locus_tag="ACG8VH_000367"
     CDS             complement(33344..36622)
                     /locus_tag="ACG8VH_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP9108958.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36893..37438)
                     /locus_tag="ACG8VH_000368"
     CDS             complement(36893..37438)
                     /locus_tag="ACG8VH_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9108959.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37450..38142)
                     /locus_tag="ACG8VH_000369"
     CDS             complement(37450..38142)
                     /locus_tag="ACG8VH_000369"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9108960.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38161..38445)
                     /gene="macP"
                     /locus_tag="ACG8VH_000370"
     CDS             complement(38161..38445)
                     /gene="macP"
                     /locus_tag="ACG8VH_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP9108961.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38458..39021)
                     /locus_tag="ACG8VH_000371"
     CDS             complement(38458..39021)
                     /locus_tag="ACG8VH_000371"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9108962.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39249..39911
                     /locus_tag="ACG8VH_000372"
     CDS             39249..39911
                     /locus_tag="ACG8VH_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP9108963.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40035..41231
                     /locus_tag="ACG8VH_000373"
     CDS             40035..41231
                     /locus_tag="ACG8VH_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP9108964.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41332..42708)
                     /locus_tag="ACG8VH_000374"
     CDS             complement(41332..42708)
                     /locus_tag="ACG8VH_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9108965.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42745..43611)
                     /locus_tag="ACG8VH_000375"
     CDS             complement(42745..43611)
                     /locus_tag="ACG8VH_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9108966.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43700..44725)
                     /locus_tag="ACG8VH_000376"
     CDS             complement(43700..44725)
                     /locus_tag="ACG8VH_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9108967.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44743..45417)
                     /locus_tag="ACG8VH_000377"
     CDS             complement(44743..45417)
                     /locus_tag="ACG8VH_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9108968.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45601..46494)
                     /locus_tag="ACG8VH_000378"
     CDS             complement(45601..46494)
                     /locus_tag="ACG8VH_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9108969.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46568..48265)
                     /locus_tag="ACG8VH_000379"
     CDS             complement(46568..48265)
                     /locus_tag="ACG8VH_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9108970.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48258..49571)
                     /locus_tag="ACG8VH_000380"
     CDS             complement(48258..49571)
                     /locus_tag="ACG8VH_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9108971.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49571..53512)
                     /locus_tag="ACG8VH_000381"
     CDS             complement(49571..53512)
                     /locus_tag="ACG8VH_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9108972.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53621..54079)
                     /locus_tag="ACG8VH_000382"
     CDS             complement(53621..54079)
                     /locus_tag="ACG8VH_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9108973.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54377..54895)
                     /locus_tag="ACG8VH_000383"
     CDS             complement(54377..54895)
                     /locus_tag="ACG8VH_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9108974.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54897..55513)
                     /locus_tag="ACG8VH_000384"
                     /pseudo
     CDS             complement(54897..55513)
                     /locus_tag="ACG8VH_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(55510..56298)
                     /locus_tag="ACG8VH_000385"
     CDS             complement(55510..56298)
                     /locus_tag="ACG8VH_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9108975.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(56360..57271)
                     /locus_tag="ACG8VH_000386"
     CDS             complement(56360..57271)
                     /locus_tag="ACG8VH_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9108976.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57312..58646)
                     /locus_tag="ACG8VH_000387"
     CDS             complement(57312..58646)
                     /locus_tag="ACG8VH_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9108977.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58854..59684
                     /locus_tag="ACG8VH_000388"
     CDS             58854..59684
                     /locus_tag="ACG8VH_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9108978.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59928..60177)
                     /locus_tag="ACG8VH_000389"
                     /pseudo
     CDS             complement(59928..60177)
                     /locus_tag="ACG8VH_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(60368..60562)
                     /locus_tag="ACG8VH_000390"
     CDS             complement(60368..60562)
                     /locus_tag="ACG8VH_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108979.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        17240 a        13150 c        11000 g        19698 t
ORIGIN      
        1 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc atctcataac
       61 tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa
      121 caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat
      181 agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc
      241 tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg
      301 tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc
      361 gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat
      421 ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg
      481 cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca
      541 tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt
      601 ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac
      661 ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg
      721 aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga
      781 tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg
      841 gaacatcgac tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg
      901 aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga
      961 ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt
     1021 cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg
     1081 ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt
     1141 ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg
     1201 catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg
     1261 tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca
     1321 tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat
     1381 cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc
     1441 ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc
     1501 caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca
     1561 gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct
     1621 acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag
     1681 gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg
     1741 tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc
     1801 ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc
     1861 acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc
     1921 catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc
     1981 gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc
     2041 tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc
     2101 caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa
     2161 tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg
     2221 gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc
     2281 ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta
     2341 taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca
     2401 ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa
     2461 agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc
     2521 accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg
     2581 attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt
     2641 aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt
     2701 caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc
     2761 catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc
     2821 tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc
     2881 atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc
     2941 taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc
     3001 ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc
     3061 atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat
     3121 ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt
     3181 gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc
     3241 aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca
     3301 tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct
     3361 ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc
     3421 aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc
     3481 aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt
     3541 tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa
     3601 tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca
     3661 gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat
     3721 tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata
     3781 ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat
     3841 tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca
     3901 tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg
     3961 aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt
     4021 actgcttttt tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac
     4081 aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc
     4141 catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg
     4201 cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc
     4261 acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag
     4321 gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa
     4381 tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg
     4441 attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca
     4501 gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata
     4561 tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt
     4621 tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg
     4681 tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt
     4741 tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt
     4801 aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca
     4861 tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca
     4921 cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct
     4981 aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc
     5041 atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt
     5101 tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca
     5161 tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc
     5221 actcatttta gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc
     5281 ttttatttat tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga
     5341 aataagccat tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc
     5401 ctttactggt ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg
     5461 gtgaagcact tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc
     5521 aataatattc acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt
     5581 caaccctgcg ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg
     5641 gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt
     5701 ttcttcaata tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa
     5761 gccagcatct tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat
     5821 gattgttgca ccatctttta attcggaaac atctttgact tttttagagt acaagcccat
     5881 tggttcgatg tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt
     5941 gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg
     6001 gttcggtaat acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa
     6061 taatggtttc acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa
     6121 tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc
     6181 taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta
     6241 catcccccta tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct
     6301 tgaacgataa atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta
     6361 ccacgctggt agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg
     6421 gccatcgcag taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg
     6481 ggaatacttt caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca
     6541 tctgctgctt ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca
     6601 taaaatggtg ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg
     6661 cctacgattg ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac
     6721 cggaaaatat tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt
     6781 ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc
     6841 attgagatta atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg
     6901 atttgtttaa aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat
     6961 tcctactcct tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt
     7021 catcaagaca gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac
     7081 aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt
     7141 tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc
     7201 taatagttct gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac
     7261 ttcttttttc ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa
     7321 ctccatcaca gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa
     7381 gacgatggtc aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt
     7441 tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc
     7501 caatgctcgt gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc
     7561 ttgtccgcgt ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt
     7621 gctctttggt acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga
     7681 ccataacagg ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc
     7741 tttgtttttt aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag
     7801 ctcaagtcca ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat
     7861 tccataaata tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc
     7921 gtttttccca ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac
     7981 atctttctct tataattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg
     8041 acgaaagctt gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct
     8101 cttccagtac ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg
     8161 tagtaagttt gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt
     8221 tacccatttt cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca
     8281 aagctttctc gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca
     8341 acttttttca agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt
     8401 tgaaacttaa gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac
     8461 cgattttatc attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg
     8521 ctttttccgc ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg
     8581 gcagattggc aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac
     8641 agtattcttt tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg
     8701 ccattttata tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg
     8761 tcctctcgta agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc
     8821 gcttttgaag ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc
     8881 ccgcttgtgt tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc
     8941 gttgaggcct tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg
     9001 tctaaccacg ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac
     9061 aattttctta cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata
     9121 tcaacccgat accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca
     9181 atgggatccc tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga
     9241 acagcagcat catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga
     9301 aacacacccg aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac
     9361 cgataaagcc gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca
     9421 catagatatt gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa
     9481 gtccttgggc cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat
     9541 caatacgtgc aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt
     9601 cagtcgtaac caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg
     9661 cgatgatgcg ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga
     9721 caagcatcgt tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga
     9781 tcaagagctt cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat
     9841 tatgtttagt tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg
     9901 gctggaaagt aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat
     9961 tctgatcata gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag
    10021 gagtcagttt ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc
    10081 caactaaata ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat
    10141 catggctaag tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta
    10201 aaatcacgcc ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc
    10261 atcccttttc ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa
    10321 gtctttccat agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat
    10381 gcagtacaaa agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca
    10441 attatctacc agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga
    10501 aggatctctt cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa
    10561 taaaaatatc gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta
    10621 gacaaaatcg agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc
    10681 acgatcatct tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa
    10741 acaccagagt ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact
    10801 agctggtctt ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata
    10861 aaaccaaaat cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt
    10921 tcgatctcaa aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa
    10981 agatagacag caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca
    11041 ttgagcaatt tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat
    11101 aataagtcgg cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg
    11161 atattcaatt cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac
    11221 agtacttctg ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat
    11281 aacttaaaca tggcaccact tccataactt tttatgataa ttactataaa aactatttga
    11341 tttaaatata caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa
    11401 ctagtaactt agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc
    11461 attttacaat gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt
    11521 atatatgaag aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt
    11581 agcttccctt ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg
    11641 cggtatctct atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca
    11701 cacgaaacaa acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc
    11761 actcatggtt atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt
    11821 catttggctg tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca
    11881 tgttatcaaa gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt
    11941 tggatttggg gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt
    12001 cttttggatt tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt
    12061 tttcgtaaga aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg
    12121 ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt
    12181 agcattgttc gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt
    12241 gttcaagctt cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc
    12301 aacagctata tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt
    12361 ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc
    12421 tcattacgta cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa
    12481 aatcgcttaa taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga
    12541 acggccaaac aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg
    12601 caagcttttg aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata
    12661 agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag
    12721 aagctacttc tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga
    12781 cctcttgttc ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt
    12841 atttattgac gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa
    12901 aggattcagc ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct
    12961 tcagcaggct tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat
    13021 tgggtatttt attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat
    13081 ttgctgaaag caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc
    13141 aaacgattgc tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca
    13201 ctatcatcgg tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga
    13261 tgatggctgg aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca
    13321 tccatgcgaa agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg
    13381 tgatgatgtt gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa
    13441 aaagtgcact tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct
    13501 tacttgacaa ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc
    13561 tcgttgttgt tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg
    13621 aaacgccaac tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt
    13681 ttgtgatctt ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg
    13741 cacattttat cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag
    13801 actttgcaaa attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc
    13861 ttctccaaac agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg
    13921 aaaaaacagt tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg
    13981 cagatatcct tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc
    14041 catatatcct gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg
    14101 caatggattg gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc
    14161 aacggctact tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca
    14221 gacattttgc tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct
    14281 ttttcccgca aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc
    14341 tgattcgctt tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc
    14401 taaccataaa aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac
    14461 tggaacttcc tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta
    14521 aaagtttgat aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac
    14581 ctgttctttt acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa
    14641 tgtaaaccat atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg
    14701 tcccatgttt taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca
    14761 aaggtgccca tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag
    14821 cagcaatcaa gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa
    14881 attttgggaa agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga
    14941 ccgtgatgga tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga
    15001 tcactacttc tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg
    15061 cgatacgcag tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag
    15121 aaatggtcga tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc
    15181 tagaattaac agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa
    15241 cagaatatat ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct
    15301 ggaaattatt caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga
    15361 ttgcttcatt tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta
    15421 ttttgcgggc attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct
    15481 tcctgttcat tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga
    15541 aaatcttctt agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga
    15601 agccaaatga attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa
    15661 atgaaaaagg actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc
    15721 cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
    15781 taaattcctt cttatttctt gaagctgacc acttctatca caacctcttt catatcttat
    15841 tacttcttca ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct
    15901 tgtgcatatt ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct
    15961 tttttatcaa gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt
    16021 ttaccttcga tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt
    16081 ggatcttttc ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca
    16141 cgtgtgtttt tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag
    16201 aaatcttgat attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt
    16261 tcgattccat tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct
    16321 ttttcccagc tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat
    16381 tgcttacctt taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg
    16441 attagtttct cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt
    16501 tgaccaagca agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg
    16561 gcgaaatcgg gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca
    16621 gtttcgcctt tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta
    16681 ccaaattcaa ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa
    16741 aaacgagaga caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt
    16801 gctctgtttt cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact
    16861 tttgtctgtt ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg
    16921 ctgactgctt gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg
    16981 tctgtacgcg gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat
    17041 gttttctttg cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca
    17101 taaggcaatg gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg
    17161 cttgtactga tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga
    17221 tttttttgaa gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt
    17281 tgtggacgga actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc
    17341 tgcacacggc ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc
    17401 aatcctacca gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat
    17461 tctttcgcag gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag
    17521 atccataacc gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca
    17581 agttcccctt ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga
    17641 gccagctgct tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt
    17701 tttttaggga tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct
    17761 tccggcattt tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct
    17821 tcataataat ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg
    17881 cttggttttt ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat
    17941 gggctgatga tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc
    18001 ttcacaccag cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt
    18061 gatatattca taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc
    18121 ttcgaaagac tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca
    18181 ccaaggctca ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc
    18241 agcttttcta gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa
    18301 aagggtcatt taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa
    18361 gtagcgataa agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct
    18421 tttgtaaagt atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc
    18481 ctgcttcgat gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg
    18541 atctgattcg tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg
    18601 atcagtaact catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag
    18661 atctgcagtg cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt
    18721 tcctgcagaa gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta
    18781 tacagcgagg gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga
    18841 acatccatct cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt
    18901 ctttttttca tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca
    18961 cgaaaaagaa agagaaaaaa gagactttca ttgataaact acatattgta gtttataata
    19021 aagagctgta gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga
    19081 aaaattattt aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac
    19141 atttatggct ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac
    19201 gttaggtaat tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac
    19261 tttcttggtg actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag
    19321 aaaattgatg ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg
    19381 cgtgactagt gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta
    19441 tatcagtaat gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt
    19501 tcttggaacc ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg
    19561 gatctctgct tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt
    19621 tttcgtcttc ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga
    19681 aaacatggtt tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg
    19741 tatgttcgtc acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag
    19801 tctgtacatt cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat
    19861 tgcttcgata cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga
    19921 caagattggg agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat
    19981 cccaatggca ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat
    20041 ctctgatgct tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca
    20101 agctgctgga cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg
    20161 gcctatgatc ggctccagtg tttccagtgc atttggttat cgcggcgtct ttctttcgac
    20221 ttctcttctt gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca
    20281 gcaagaaact gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc
    20341 tcatagacaa taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg
    20401 cagtgttttt gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc
    20461 caaggtttct tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact
    20521 tcctggtact aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc
    20581 ttcttcgatg gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac
    20641 tttcggttca ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt
    20701 gtttcgcgcc aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag
    20761 gactccgccg caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg
    20821 tccagtagct gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt
    20881 ggttgctata cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa
    20941 aggcgcatat acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat
    21001 tttttctaaa gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca
    21061 gatgattttt gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc
    21121 ttcttcttcc ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata
    21181 taattgctga taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta
    21241 tagagctaaa agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac
    21301 ggtttgataa agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata
    21361 gtccaatggt tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc
    21421 aacgagttcg tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt
    21481 cttttcccat ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc
    21541 ccccttatat cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac
    21601 aaatcagccc aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag
    21661 cgatccagta gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa
    21721 atcctgctaa aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt
    21781 gttttttgat tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac
    21841 aaatcaaaat tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta
    21901 agaaagtcaa taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg
    21961 catttttttt attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca
    22021 tcgttgtttc tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca
    22081 agccgatatt gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca
    22141 tagcaggttg tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg
    22201 aagcaaggat atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt
    22261 ctgaacgttt cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc
    22321 acatcataac catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc
    22381 cattcactag attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa
    22441 atgcgataaa tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga
    22501 ttgtattgat gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa
    22561 taaaaatact tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa
    22621 attctcccat ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca
    22681 accagtaacc ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac
    22741 cgaaaatacc accatctaga tctggacgct tttctcctaa atgattcagc gataatacca
    22801 aagctaaaat accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag
    22861 aactagccag atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca
    22921 gagcaaccag tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca
    22981 cttataaaat tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc
    23041 ttaacaaaaa gcaactttat tcatattatt ggatattatg aactttttac acaagtattt
    23101 caagtataaa atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag
    23161 aaaaggtgtt tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta
    23221 ttgtaaaact tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat
    23281 tccatcataa aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac
    23341 gacttctgca cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata
    23401 acggaatttc gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat
    23461 ttcacttgca tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata
    23521 taatgtgttt gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc
    23581 gccaccgatc cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg
    23641 tcctttgact tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct
    23701 caaaaattgt ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt
    23761 agcagtcgca aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc
    23821 tagagggaac atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg
    23881 atctttatta ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac
    23941 ttgcgcataa tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa
    24001 tgctttgaat ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg
    24061 gtattcggct aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga
    24121 gtaataagga atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc
    24181 cgtgcataca ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc
    24241 gtatccttgt tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc
    24301 tacgacaacg cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc
    24361 tgaaacatct acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt
    24421 ctgaaaattt catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca
    24481 gtacacaaac atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa
    24541 attggcatac tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt
    24601 gaaaaactat ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc
    24661 ttttcttttt agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa
    24721 gggaggcaaa agatacatga attactttat cagtgatatg cactttttcc atgaacgctt
    24781 attaggaaaa aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca
    24841 gttgatcact agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga
    24901 ccttgctatg catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca
    24961 actgcaagga acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta
    25021 tttagaaaaa aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt
    25081 aggtgccatc gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct
    25141 cgggcctaac gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat
    25201 cgagaacgat gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact
    25261 accatttggc actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa
    25321 gtacgatagg tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac
    25381 tcatattttt catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt
    25441 gtaacaagac ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc
    25501 ttttaccagt tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa
    25561 aacgattccg cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa
    25621 acgatcagct aatggtcctg caaataataa tccaattgga cctgccaggt tcatcaaaga
    25681 gttgaagttc cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc
    25741 catcagcagt gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa
    25801 gtacaagaac ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat
    25861 aatgatgtag gctataataa taggtttcat tcggtctttc catgttccta taaaactgat
    25921 cagtgcaccg cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc
    25981 aacggaacca ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa
    26041 caacagcatc acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa
    26101 ttgctgcagc ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg
    26161 agaaactttt gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac
    26221 atctaataaa ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg
    26281 cgcaatgatg aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg
    26341 agaagatgga acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc
    26401 gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt
    26461 tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag
    26521 agctttttta tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg
    26581 aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa
    26641 ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga
    26701 taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat
    26761 aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga
    26821 ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt
    26881 acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat
    26941 aaaaaagaca actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt
    27001 caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct
    27061 aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt
    27121 gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat
    27181 atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa
    27241 tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt
    27301 atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt
    27361 tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt
    27421 atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag
    27481 acatctgttc atataagaat gacttcacta gttatttttt gacagcacga actaattgat
    27541 caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt
    27601 cttttagtat cggtgtatct tctccataca ttgcttctaa tacgtgtgaa ggctcgatat
    27661 ttcctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca
    27721 gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg
    27781 ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt
    27841 gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa
    27901 ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt
    27961 cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac
    28021 cgacgccttt tggcccattg attttatgag cagagatact taataaatcg attcctaatt
    28081 cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg
    28141 gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt
    28201 tcccgtacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga
    28261 tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta
    28321 aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc
    28381 cttctttttg acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc
    28441 cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg
    28501 ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa
    28561 acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg
    28621 ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca
    28681 cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt
    28741 tcatgggcat agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct
    28801 cacttctgtc acaacttctt tccctctttt ttttgtttat tcgaattctt tttcttccag
    28861 acggaataac ggactcatcg gtgtattttc atggatgcgt ttgaccgctt cacccattaa
    28921 ttctgaacat gtaacgattg ttaagttttc tggatgacga ttttcttctg tgaaaacaga
    28981 atcagtgata caaatatctt tgattggtgc attgtctaaa atacttttcg catgatcgga
    29041 caataatcca tgtgacgcac aggcatatac ttcttttgcg ccgttttctt ttaatacatt
    29101 tgctgctcgt gcaagcgttg ctcctgtgtt caagatatca tctaccatga tacatgtctt
    29161 tccttgaaca tcaccgatca cataaccacc ctcagttgaa gcatcatctg catgatctac
    29221 gatggctaat gtgctgttca aatattctga aagactacgt gcacgctgaa caccgctgtt
    29281 ttttggcgaa acaactacga tgtcatctcc cgtcagacct aattgacggt aataatgagc
    29341 aaacaacggc atcgtaaata aattatctac aggaatatca aagaatcctt gtacttgaac
    29401 cgtgtgcaga tctaaagtca gtactcgagt tgcccctgct tcttcgatca agttagcgat
    29461 caatttcgca gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag catacccata
    29521 gtaaggcaat acgacattga tcgttttcgc acttgctcgc ttcattgcat cgatcatgat
    29581 cagtagttcc atatagtaat cattgacagg tgcattcgtt gattgaacga tatacacatg
    29641 atcccctcga atactttctt cgatattgat ggaaatctcg ccatcactaa attgcttcac
    29701 gacgctttta cctaattctg ttccgaacac tttcgcgatt ttttcagcta atggacggtt
    29761 gccattcaaa ctaaaaatct tcaacgtgtc atcctgatac ttattactca tgtagtcctc
    29821 cccaaaactt ctctattctt cgatagttta acatattttt ccaagatttt ctgttaattt
    29881 cagcaaattc ttaaggaagt gaaactgttc cgttactcca actggcagga atgactttat
    29941 gataatcgcc ccagttgtcg tgtactttgt aataacgttc tcctgtttca gtttcaaaat
    30001 aagcgtacgc tgtcacccaa tgattgccat atgtactgcc aagtactttc aaaataccaa
    30061 tcatcacagg tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag
    30121 ttgctcgtgc acgctcatga ttgccattct tctttaaaaa actagaaatc cctgtactaa
    30181 cctgaaaagg aatcgtagga agtcctagtg gttggatctg gctacgcaat gccttcgtca
    30241 aaaccaacga atcagccgta tttttcttgc gaatctcatt tggaagcatc caccccttac
    30301 gatagtcttg ataataggca aggagaactg ctgccgcgta agtaccacaa agatagcctg
    30361 agggggtagc ccatttctta tacctcaaaa aacgttcttc tggcaatccc acccagcaat
    30421 ttgcgagttc ctttcctgac cttttttctt cgatcaaaac gggaatatct tcttttctgt
    30481 tgatccatct agataactcg attggctctt cattgatcag cttccatttt ttacaagaaa
    30541 gaccctcttg ataagtatat tttgctggag aatattctac cggatgtcct gattgatcga
    30601 taatcacata actttgatct tcaaaaacca gagctaaaaa aagcaattta ctttttcttg
    30661 actgcagctg ctgagtgctg gctaattttt taattttttt atctactttg aaatataaag
    30721 gtttatccac tacttgattc agttgggatc gcctcctttt tttcattata agtgaaggat
    30781 tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa
    30841 ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa
    30901 caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc
    30961 aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga
    31021 ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga
    31081 aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc gaagaaatga
    31141 gttgcatgct caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga
    31201 cgaattgaat aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct
    31261 ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc
    31321 tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat
    31381 aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca
    31441 ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa
    31501 aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt
    31561 tattaaatac acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac
    31621 tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac
    31681 agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag
    31741 tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg
    31801 aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga
    31861 tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt
    31921 agaacaaatg gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg
    31981 gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt
    32041 aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca
    32101 gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt
    32161 cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa
    32221 tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc
    32281 attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc
    32341 tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt
    32401 gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat
    32461 cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc
    32521 ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc
    32581 agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa
    32641 aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc
    32701 aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga
    32761 cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt
    32821 ggtcagcttc aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat
    32881 ttcggcttat ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa
    32941 gctgcttagt ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    33001 aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa
    33061 tcagcgtcct gacctgagta ttagagaaaa aaacggaaaa atagttcctc atattttgcc
    33121 gtttgttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gagattgtga
    33181 aaaagctgtc ctgcatcaag taataagaac agccaatcaa aagcagcaag gagaacctca
    33241 tctataaaaa gatagaagga agtgagacat ctattggtct catttccttc tatttttact
    33301 ttcctcagtc aattgtttta catcataaaa ggagaaattc cacttatttt tttcgctgtt
    33361 tcatgacata aacaactaca ccgattaaca aaattcctcc agctaatgct agcaacgggt
    33421 tattaaattc tcccgttttt gggagtcctt gagtgccatt tttttcactt tcttttgctg
    33481 tcctatccgg atgagaaggt tttccatttg gttttccatt tggtgattca tttcctggat
    33541 tttctctttc tggtttgtca tttcccgggg gcacatcggc agtttctcgc acagtgatcg
    33601 ctttttgctg ggtaacagat tctgtatgag cagaccattt ttccccatca aatatttccg
    33661 aacgataagt gacttctagc gtatattctc cttttgaagg tgctgaaaaa atagcggtat
    33721 gattagaaaa ttgagcactt gactgtgttt caacgatttg gatcttttct gggaaataac
    33781 gcatatcttc aaagacaggc gttccttgat ttggtgtgaa agttgacaca aaaactgtaa
    33841 aaggcttcag ttcaatgata tttccttctg cattctccgc cacaccattt gcttcttgta
    33901 taataatcgg attattttgt tcatttggtt ttgtctgatt tggttcttgt acgattgcat
    33961 actctggata ttccgaagac ggtgaggttt ctccgtcaaa aatagcacct acagataatg
    34021 gaagaccatc cactctttcc ttttctgttt gacgattccc cggtttaatc tgcatcagtc
    34081 catgaacatg gatacttcca ttttcatagc gcgtaacagg ctgctgacct tctttcgaac
    34141 atgtatttag agactcgatc caatcttttg atatggtatg tccaagagta ttttctgatt
    34201 gtttctctcg ttcattccaa tagtagttca acgagtggta gatttgatct tcgttttgat
    34261 tcaatgggcg gatctgctct ctcatctcaa gattctctgt tcggaaagat aagataccag
    34321 aagcatggta gtctgtgttt cctgctgaag aggtataaag agccacatta tatgaaccgt
    34381 tattaaaact tgtactgcta ttgacgataa tatctgttcc gctattggaa tcgattcctt
    34441 tcgcaagatt ttcgaaagac aaactattga tcaattcatg agggcccgtt aaagaagaac
    34501 cgcccatttt gaatccatta ccgttacctt ctccctcaat tcctggtacc cagccgtttc
    34561 gataagcaac tgagtttttg atgattactt ttccgatcga acctgtttca tttttagcaa
    34621 aaaggtccca accgtcatct gcattatgat acgcgataca accgtcaaat acatttcctt
    34681 ctcctattgt cagtttggcc gcaaatccat cggcatcttc aaatcctgga tcggcatttt
    34741 caaaagatgt acagttcttg actaagttat gcgcgggcca tgcttcgaaa ggatcatttg
    34801 agctaccgct gatttggatt cctgtatttc cattccgata agcatgaatc tcttcgatca
    34861 aattatgatt tccagaaatc tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa
    34921 accctcgtaa accccagtag tgactgaaca gtgtcatccc atttccagtt cctttaaaat
    34981 caagtacagg acgcttgttt tctggatttt tctctgcaat caactgaatc gggttttctc
    35041 ttgtcccgtt aacgcctttt ggaatcgtca atcctctttc gaaggaataa gttccaggcg
    35101 ccatgacgat tgtctgccca gcatatgcgt atctcaatgc ttgtgtcaga gataatggat
    35161 tttgtctgct tccgtttcct gcatctgacc cttctggtgt tacataaact gtcgaatatt
    35221 taggtttacg gtagtccact gtatgaagaa tctcttttgt ttcataacta gtcatctcta
    35281 cgtattcttc tggcggatag tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc
    35341 cttccatcag ttttgtctga taatcaaatt cccgattcgc agtgatagcc aagttttttg
    35401 cgttcttaag cacctctcct gttgctttat ttttaattgt caataatccg tcagcatttg
    35461 ctctaaagga catagtatga tcttggtttc ctgaatgatt actagaggtc acagtgtaaa
    35521 tcggatcaat caagcgagta ggtctttctt gcccaggagg atctgtcgca ggatctgagt
    35581 aagcaacttc gattccttct accttgatcg tcatattcct tgctgcagca aatcctgcat
    35641 aaataaccga ttcatctgaa gcgtataatt tttcccagtc atacatgatg tcagaagcca
    35701 tttcttctcc tgtttcagga tcgtaataat atgcctctaa accagtattc gtttttttca
    35761 tctctacgtc cagtttagta atcgagttcg tggccggaaa cagattccca tgaccgtttc
    35821 cgaatatatt ataagaacct gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg
    35881 ttgtttccac tgagacactc ccaggagctg gcgcagcttg cggatcttca gatgatattc
    35941 ctgtgattgc ccgtgtcccc aatccaagtc gcatcgtata tttgctgccg ctcgtgttga
    36001 ccacttcttg tctatccgag tcccagacat attcgatccg actgcctagt atggcaaatg
    36061 aatttgaata aaatgcctgc ccaaacaaat gattttttgg tactgcgtct cttaccatta
    36121 aagcaaatcc ttcttgggca ttcgtatagg tccaagattc tatttcgatc gtcgcccgca
    36181 tcctaaagtt gatgtctttg gggattgctt gataataata ggtcattcca tctgatccgc
    36241 ttgactggaa ttttccgccg ttattcgtgg aagagagccg cacgccgtct tctaatggtt
    36301 caatggcata cctactagca cttgtgcttt ctccgaaaac aacccctttc cactcttcat
    36361 ttgcttcttc agattgcttt tttgtctttt cattttctga ttctttcatc ataggatcat
    36421 ttccttcttc cgtatccata tcttcactag ttcttggtat ttcctccatt gttgacatcc
    36481 cttcaatgtt cacgtgtgca ttttcttcgt tcacagactg ttgttcgtta gcagcaactt
    36541 caacgacaga aattaaaacc ggggtactga gcataaggag attcatcatt agcgcactgt
    36601 attttatacg tttttttaac aagtgtattc ctcccgtaac aaattagaaa gcgtttactt
    36661 aaagtattac ctaatttatt tttactgtca ataaaaaaca aaattataat aagtgtattt
    36721 tataaaaaga aaaaatataa cttaagttgt tgataataaa gagatttatt aacggaaaaa
    36781 gaataagttt ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat
    36841 gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa
    36901 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
    36961 aacttgccac ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
    37021 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
    37081 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
    37141 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
    37201 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
    37261 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
    37321 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
    37381 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
    37441 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
    37501 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
    37561 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
    37621 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
    37681 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
    37741 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
    37801 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
    37861 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
    37921 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
    37981 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
    38041 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
    38101 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
    38161 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
    38221 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
    38281 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
    38341 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
    38401 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
    38461 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
    38521 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
    38581 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
    38641 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
    38701 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
    38761 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
    38821 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
    38881 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
    38941 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
    39001 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
    39061 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
    39121 ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta
    39181 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
    39241 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
    39301 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
    39361 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
    39421 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
    39481 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
    39541 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
    39601 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
    39661 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
    39721 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
    39781 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
    39841 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
    39901 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
    39961 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
    40021 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
    40081 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
    40141 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
    40201 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
    40261 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
    40321 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
    40381 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
    40441 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
    40501 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
    40561 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
    40621 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
    40681 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
    40741 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
    40801 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
    40861 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
    40921 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
    40981 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
    41041 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
    41101 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
    41161 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
    41221 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
    41281 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
    41341 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
    41401 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
    41461 gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
    41521 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
    41581 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
    41641 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
    41701 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
    41761 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
    41821 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
    41881 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
    41941 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
    42001 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
    42061 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
    42121 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
    42181 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
    42241 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
    42301 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
    42361 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
    42421 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
    42481 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
    42541 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
    42601 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
    42661 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
    42721 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
    42781 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
    42841 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
    42901 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
    42961 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
    43021 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
    43081 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
    43141 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
    43201 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
    43261 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
    43321 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
    43381 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
    43441 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
    43501 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
    43561 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
    43621 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
    43681 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
    43741 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
    43801 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
    43861 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
    43921 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
    43981 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
    44041 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
    44101 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
    44161 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
    44221 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
    44281 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
    44341 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
    44401 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
    44461 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
    44521 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
    44581 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
    44641 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
    44701 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
    44761 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
    44821 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
    44881 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
    44941 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
    45001 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
    45061 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
    45121 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
    45181 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
    45241 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
    45301 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
    45361 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
    45421 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
    45481 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
    45541 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
    45601 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
    45661 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
    45721 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
    45781 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
    45841 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
    45901 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
    45961 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
    46021 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
    46081 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
    46141 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
    46201 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
    46261 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
    46321 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
    46381 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
    46441 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
    46501 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
    46561 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
    46621 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
    46681 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
    46741 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
    46801 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
    46861 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    46921 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    46981 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    47041 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    47101 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    47161 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    47221 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    47281 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    47341 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    47401 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    47461 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    47521 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    47581 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    47641 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    47701 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    47761 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    47821 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    47881 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    47941 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    48001 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    48061 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    48121 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    48181 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    48241 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    48301 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    48361 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    48421 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    48481 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    48541 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    48601 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    48661 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    48721 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    48781 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    48841 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    48901 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    48961 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    49021 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    49081 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    49141 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    49201 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    49261 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    49321 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    49381 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    49441 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    49501 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    49561 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    49621 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    49681 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    49741 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    49801 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    49861 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    49921 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    49981 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    50041 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    50101 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    50161 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    50221 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    50281 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    50341 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    50401 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    50461 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    50521 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    50581 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    50641 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    50701 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    50761 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    50821 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    50881 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    50941 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    51001 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    51061 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    51121 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    51181 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    51241 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    51301 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    51361 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    51421 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    51481 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    51541 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    51601 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    51661 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    51721 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    51781 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    51841 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    51901 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    51961 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    52021 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    52081 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    52141 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    52201 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    52261 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    52321 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    52381 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    52441 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    52501 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    52561 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    52621 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    52681 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    52741 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    52801 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    52861 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    52921 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    52981 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    53041 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    53101 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    53161 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    53221 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    53281 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    53341 acttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg
    53401 ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa
    53461 tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt
    53521 cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt
    53581 ggttatctat tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg
    53641 ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc
    53701 cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat
    53761 aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat
    53821 cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc
    53881 attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta
    53941 agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat
    54001 gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct
    54061 tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa
    54121 caatcaacaa aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa
    54181 tgatatcctt tttagttaaa aaacaccaat tatacaacat ttattattaa aattaaatta
    54241 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
    54301 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
    54361 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
    54421 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
    54481 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
    54541 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
    54601 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
    54661 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
    54721 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
    54781 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
    54841 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
    54901 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
    54961 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
    55021 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
    55081 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
    55141 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
    55201 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
    55261 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
    55321 gaaccgagct ttttctcttc atttttttgt aatgataaat catatacaga actgttcgac
    55381 ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga
    55441 taagacgtgg aatccattca atgatttcta agaaataatc attggctttt ttaccaatcg
    55501 atcgattcat catccttttg aacctcctgt caaatccatc ccttcaatga aataacgttg
    55561 tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt gttgctcctg ccatcagata
    55621 attccattgt gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta acgtgtattt
    55681 ttcttgatct tggatatata acagaggacc taagaaatca ttccaagctg cgttgaagga
    55741 attgatcgca atggtaatca aggcaggttt cgtcaatgga agcatcaaat gactccaaat
    55801 atacaaatga ttcgctccgt cgatttcagc agcttcgatc aattcattat tgatcgataa
    55861 gtaaaattga cgcatcagaa atatattgaa cgcattgccg aagaacgatg gaacgatcag
    55921 gggcaaataa gtacctaccc agccgatctt ggagaataat acatattgag ggatcatcgt
    55981 tacaaacccc gggatcatca tcgtagaaag aaccaaagca aacatcagct ttttcccagg
    56041 aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt gaaaggacat taccgatcac
    56101 tactagcaca gtgatgaata atgtatttct tgcatagcgc caaaaaggaa aggcggcaat
    56161 cgtatccaga tagttttccc atacgatttt ttctgggaaa aaggtcggag gataacgaac
    56221 gatttcttcc attggtttca atgaagtaaa caccatccaa agcaatggaa tgatcaaaat
    56281 gattcctatt actgtcatga ggacataact tagtattttt ttgtttgccg attcacactg
    56341 gtctttttga aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg
    56401 caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag
    56461 ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa
    56521 aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa
    56581 aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta
    56641 atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt
    56701 catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag
    56761 aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag
    56821 ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa
    56881 gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag
    56941 ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac
    57001 tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt
    57061 tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca
    57121 tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta
    57181 gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt
    57241 cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag
    57301 tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc
    57361 aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat
    57421 gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg
    57481 tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc
    57541 tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact
    57601 tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat
    57661 ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct
    57721 gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa
    57781 taattgatat tttgggcacg catggctact aatccggaaa taaatggatc agatacacca
    57841 gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct
    57901 tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg
    57961 tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat
    58021 aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct
    58081 gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta
    58141 aatatctttt tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga
    58201 tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt
    58261 tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt
    58321 atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc
    58381 cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg
    58441 aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag
    58501 aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg
    58561 gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc
    58621 aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg
    58681 gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa
    58741 aaaaattatt aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt
    58801 aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta
    58861 gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga
    58921 aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc
    58981 tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg
    59041 caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg
    59101 gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta
    59161 tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca
    59221 gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact
    59281 ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa
    59341 tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc
    59401 atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag
    59461 tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact
    59521 tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg
    59581 aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac
    59641 cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa
    59701 acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa
    59761 ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct
    59821 aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac
    59881 ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca
    59941 ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc
    60001 gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca
    60061 aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg
    60121 attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta
    60181 ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta
    60241 tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct
    60301 tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca
    60361 atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata
    60421 aagccgtata aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc
    60481 cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga
    60541 atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat
    60601 tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc
    60661 cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc
    60721 taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata
    60781 cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg
    60841 agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac
    60901 tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct caactacatg
    60961 cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat
    61021 tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta
    61081 taaaataa
//
LOCUS       DAZKCC010000006        60870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000006 DAZKCC010000000
VERSION     DAZKCC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.006"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1184)
                     /locus_tag="ACG8VH_000391"
     CDS             complement(33..1184)
                     /locus_tag="ACG8VH_000391"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9108980.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1197..1634)
                     /locus_tag="ACG8VH_000392"
     CDS             complement(1197..1634)
                     /locus_tag="ACG8VH_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9108981.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1649..3415)
                     /locus_tag="ACG8VH_000393"
     CDS             complement(1649..3415)
                     /locus_tag="ACG8VH_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP9108982.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3679..4356
                     /locus_tag="ACG8VH_000394"
     CDS             3679..4356
                     /locus_tag="ACG8VH_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9108983.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4359..5486
                     /locus_tag="ACG8VH_000395"
     CDS             4359..5486
                     /locus_tag="ACG8VH_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9108984.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5686..6126)
                     /locus_tag="ACG8VH_000396"
     CDS             complement(5686..6126)
                     /locus_tag="ACG8VH_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9108985.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6137..8170)
                     /locus_tag="ACG8VH_000397"
     CDS             complement(6137..8170)
                     /locus_tag="ACG8VH_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFP9108986.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8172..9875)
                     /locus_tag="ACG8VH_000398"
     CDS             complement(8172..9875)
                     /locus_tag="ACG8VH_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9108987.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9969..10919)
                     /gene="manA"
                     /locus_tag="ACG8VH_000399"
     CDS             complement(9969..10919)
                     /gene="manA"
                     /locus_tag="ACG8VH_000399"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP9108988.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10958..11983)
                     /locus_tag="ACG8VH_000400"
     CDS             complement(10958..11983)
                     /locus_tag="ACG8VH_000400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9108989.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12192..13061)
                     /locus_tag="ACG8VH_000401"
     CDS             complement(12192..13061)
                     /locus_tag="ACG8VH_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9108990.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13520..15412)
                     /locus_tag="ACG8VH_000402"
     CDS             complement(13520..15412)
                     /locus_tag="ACG8VH_000402"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9108991.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15409..17136)
                     /locus_tag="ACG8VH_000403"
     CDS             complement(15409..17136)
                     /locus_tag="ACG8VH_000403"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9108992.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17152..17802)
                     /locus_tag="ACG8VH_000404"
     CDS             complement(17152..17802)
                     /locus_tag="ACG8VH_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP9108993.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18006..18302
                     /locus_tag="ACG8VH_000405"
     CDS             18006..18302
                     /locus_tag="ACG8VH_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9108994.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18474..19094
                     /locus_tag="ACG8VH_000406"
     CDS             18474..19094
                     /locus_tag="ACG8VH_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9108995.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19186..20352)
                     /locus_tag="ACG8VH_000407"
     CDS             complement(19186..20352)
                     /locus_tag="ACG8VH_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9108996.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20364..20717)
                     /locus_tag="ACG8VH_000408"
     CDS             complement(20364..20717)
                     /locus_tag="ACG8VH_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9108997.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20845..21867
                     /locus_tag="ACG8VH_000409"
     CDS             20845..21867
                     /locus_tag="ACG8VH_000409"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP9108998.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21886..22047
                     /locus_tag="ACG8VH_000410"
     CDS             21886..22047
                     /locus_tag="ACG8VH_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9108999.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22306..22740)
                     /locus_tag="ACG8VH_000411"
     CDS             complement(22306..22740)
                     /locus_tag="ACG8VH_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109000.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22850..24709)
                     /locus_tag="ACG8VH_000412"
     CDS             complement(22850..24709)
                     /locus_tag="ACG8VH_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9109001.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24710..24850
                     /locus_tag="ACG8VH_000413"
     CDS             24710..24850
                     /locus_tag="ACG8VH_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP9109002.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24827..25987)
                     /locus_tag="ACG8VH_000414"
     CDS             complement(24827..25987)
                     /locus_tag="ACG8VH_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9109003.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26294..27718)
                     /gene="araA"
                     /locus_tag="ACG8VH_000415"
     CDS             complement(26294..27718)
                     /gene="araA"
                     /locus_tag="ACG8VH_000415"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9109004.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27734..28432)
                     /locus_tag="ACG8VH_000416"
     CDS             complement(27734..28432)
                     /locus_tag="ACG8VH_000416"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9109005.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28389..30050)
                     /locus_tag="ACG8VH_000417"
     CDS             complement(28389..30050)
                     /locus_tag="ACG8VH_000417"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9109006.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30268..31668
                     /locus_tag="ACG8VH_000418"
     CDS             30268..31668
                     /locus_tag="ACG8VH_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP9109007.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31920..32999)
                     /locus_tag="ACG8VH_000419"
     CDS             complement(31920..32999)
                     /locus_tag="ACG8VH_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9109008.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33085..34044)
                     /locus_tag="ACG8VH_000420"
     CDS             complement(33085..34044)
                     /locus_tag="ACG8VH_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9109009.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34056..35567)
                     /locus_tag="ACG8VH_000421"
     CDS             complement(34056..35567)
                     /locus_tag="ACG8VH_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFP9109010.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35589..36152)
                     /locus_tag="ACG8VH_000422"
     CDS             complement(35589..36152)
                     /locus_tag="ACG8VH_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9109011.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36158..37021)
                     /locus_tag="ACG8VH_000423"
     CDS             complement(36158..37021)
                     /locus_tag="ACG8VH_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109012.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37035..37931)
                     /locus_tag="ACG8VH_000424"
     CDS             complement(37035..37931)
                     /locus_tag="ACG8VH_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109013.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38106..39395)
                     /locus_tag="ACG8VH_000425"
     CDS             complement(38106..39395)
                     /locus_tag="ACG8VH_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9109014.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39367..40884)
                     /locus_tag="ACG8VH_000426"
     CDS             complement(39367..40884)
                     /locus_tag="ACG8VH_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP9109015.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41082..41972
                     /locus_tag="ACG8VH_000427"
     CDS             41082..41972
                     /locus_tag="ACG8VH_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP9109016.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42045..42869)
                     /gene="nadE"
                     /locus_tag="ACG8VH_000428"
     CDS             complement(42045..42869)
                     /gene="nadE"
                     /locus_tag="ACG8VH_000428"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9109017.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42881..44353)
                     /locus_tag="ACG8VH_000429"
     CDS             complement(42881..44353)
                     /locus_tag="ACG8VH_000429"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9109018.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44990..45352)
                     /locus_tag="ACG8VH_000430"
     CDS             complement(44990..45352)
                     /locus_tag="ACG8VH_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9109019.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45575..46327
                     /locus_tag="ACG8VH_000431"
     CDS             45575..46327
                     /locus_tag="ACG8VH_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9109020.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46467..47390)
                     /locus_tag="ACG8VH_000432"
     CDS             complement(46467..47390)
                     /locus_tag="ACG8VH_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9109021.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47405..47677)
                     /locus_tag="ACG8VH_000433"
     CDS             complement(47405..47677)
                     /locus_tag="ACG8VH_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109022.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(48147..49772)
                     /gene="groL"
                     /locus_tag="ACG8VH_000434"
     CDS             complement(48147..49772)
                     /gene="groL"
                     /locus_tag="ACG8VH_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9109023.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(49823..50107)
                     /gene="groES"
                     /locus_tag="ACG8VH_000435"
     CDS             complement(49823..50107)
                     /gene="groES"
                     /locus_tag="ACG8VH_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9109024.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            50332..50994
                     /locus_tag="ACG8VH_000436"
     CDS             50332..50994
                     /locus_tag="ACG8VH_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9109025.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(51070..52362)
                     /locus_tag="ACG8VH_000437"
     CDS             complement(51070..52362)
                     /locus_tag="ACG8VH_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9109026.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            52532..53161
                     /locus_tag="ACG8VH_000438"
     CDS             52532..53161
                     /locus_tag="ACG8VH_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9109027.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(53264..54073)
                     /locus_tag="ACG8VH_000439"
     CDS             complement(53264..54073)
                     /locus_tag="ACG8VH_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9109028.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(54128..54997)
                     /locus_tag="ACG8VH_000440"
     CDS             complement(54128..54997)
                     /locus_tag="ACG8VH_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP9109029.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(54998..56314)
                     /gene="yycH"
                     /locus_tag="ACG8VH_000441"
     CDS             complement(54998..56314)
                     /gene="yycH"
                     /locus_tag="ACG8VH_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP9109030.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(56311..58146)
                     /gene="walK"
                     /locus_tag="ACG8VH_000442"
     CDS             complement(56311..58146)
                     /gene="walK"
                     /locus_tag="ACG8VH_000442"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP9109031.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(58151..58855)
                     /gene="yycF"
                     /locus_tag="ACG8VH_000443"
     CDS             complement(58151..58855)
                     /gene="yycF"
                     /locus_tag="ACG8VH_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP9109032.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(59045..59905)
                     /locus_tag="ACG8VH_000444"
     CDS             complement(59045..59905)
                     /locus_tag="ACG8VH_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9109033.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(59892..60461)
                     /locus_tag="ACG8VH_000445"
     CDS             complement(59892..60461)
                     /locus_tag="ACG8VH_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP9109034.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
BASE COUNT        17573 a        12744 c        10494 g        20059 t
ORIGIN      
        1 tttcgtcaca ttcccctttt tcgtatccta ttttaagcga cttgcttggc ataacggttc
       61 acgttttgct tgatattccc gattgttttc ggatcattga ttcctgtgac ttcttgaatc
      121 aattgttcca agttttcatg gatcttcatt tcatgcaatt gacggctttg ttcatcttca
      181 ggatcatagt aattaaaaat aatccccatt gttgccgtca aatgtgggca agtcaagtta
      241 tgttcttgta gttctctcac tggacgtgta aatctttctt gataacctaa cttacgtaat
      301 ggtgtccgag cgacacgtgt gatcgcatca gagatatttt tgttttggaa gcgatgaatg
      361 attttttcaa tatacgcatg atgctgctct gcatcaaacc cccatttggc aatcagcaaa
      421 cttcctgttt cctgcaaaac tgattttaat tgtgccacaa ctaacgcatc ttgcattgct
      481 tcatcaatgg tctggtaacc taacaaggca cctgtataag cgacagtcgc atgaccagta
      541 ttgacactaa atagtttgcg ttcgatatat ggttctagat catctacata taaaacccct
      601 tttaattgga tttttttatt ttttctttgg ctatcgtcaa tgacccattc tttgaaaggt
      661 tccacttgga cgaataaagg atcttcatgg tgttgtaatg ggacgattcg atcgactgct
      721 gcatttggaa agccgatata tttgtcagta aatgatgttt ccggaagata tttatacact
      781 tctttttcta gaaatgctga accgccaatc atattttcac aggcaatgat atctaaagat
      841 gttgtgcttc cttccttttc tctttcttgg ataccttttg caatcaactc agcaataaaa
      901 ggtaagatat tcgggccaat ggcggtagta actaaatctg cttcagcgat ttcttggatt
      961 actttttctg gatttgtttg attgttgatt cctttgacat tttttacagt gattttttct
     1021 tttgattcat ctgctaattc gatttcgtat tcttttcttt catttaaagc atcaatgatc
     1081 gtgtgattca catctacgaa agtaatctca aatccattag ctgccaaaac ctctccaata
     1141 aatcctcgac cgatatttcc tgcaccaaaa tgtactgctc tcatttttct ctctccttaa
     1201 gcaattgcta gattttccgt tacttcttct ttgctgagcg cgtctgctag ctggacaaca
     1261 ttgtccatat cactgcaata aagtgcgatt tgctgtacaa gttgcaaatg ctcatctccg
     1321 actcctgcaa taccgaacaa aacagtcgca atcttttctt cttcttcggt accaaaatct
     1381 acaccatctg gtacttgaac gacacagatg ccggattttt tcacatattt ttttgcttca
     1441 tctgttccat gaggaatcgc gataaaattc cccatataaa cagataacat tttgtctcta
     1501 tctaccatgg catcgatata ttcctcatca acgccccctg cttctacaag catctgaccg
     1561 caatagcgga tcgcttcttc tttggtttta aaagatcgat tgagctcgat catgtcgaat
     1621 ggcaaacttt gcatgctgtt ttcctccctt aagctgtctg tttcaaatat tgtgcaatcg
     1681 attcgtattt ttgcggattg accatgctgt tgactgataa atgaatggat tgaggtgcag
     1741 ccatctgtac tttttccgct aaactttctt tggctacgat cagtgaatca ggttcatctt
     1801 ttaaatcttg gatattgact tttcttaacg ggatagttaa atggttcttg tctaatatat
     1861 ctttgagcac ggtcatcccc attgttgctg atccggaacg atcatcgtgg acaagtagaa
     1921 tctctttgat ttgtttgatg tttgctgaaa gaaactcttc tgctggctca ttctcaattt
     1981 cagaaactga tgttttctgc ttgctgctta attcttgaat gatttcatca tatcgaggtg
     2041 aattcaaaaa gttttctact gagacaaaac gcgcgctcgg tgctttttgt tttgctcttt
     2101 cttgtagttc gacctgagta acgatcaatg tattcgagtc atctgtcagt tggctgattg
     2161 cttgattggt caccgtttga ggcaagtctg cttcctgtac tttttttctc aagatcgaag
     2221 cgcccatcgc acttgatccc attcctgcat cacaagcaaa aatgattttt tgtacgccag
     2281 agaatgtagc tccttgagga ataccttggg cacctttgga agcttgctta gctgcctttg
     2341 tctcttgaac agatttttcg aagtcttcat ttgtatcttt cgcatccgct ttcaaaatta
     2401 ctgcagaaat accaaacgaa acagctgcgg caacaatcac gcccaaaata acaggaaggt
     2461 aagaattgaa cggtgtcatc gctagaatcg caatgatcga ccctggagaa gctggcgctc
     2521 ttagtccagc atccatcaat tggaaaatga aacttcctga cattcctccg cctataactg
     2581 ataagaataa taacggtttc atcataacgt aaggaaaata gatttcatgg atcccgccta
     2641 aaaagtgaat gatcatggct cctggtgctg aagattttgc tgctcctttg ccaaacaaag
     2701 taaaagcaag taatacgcca agtcctggac caggatttgc ttcaagcaag aatagaatag
     2761 attttccagc ctctgctact tgttcagttc ccaatggtgt caaaatccca tgattgattg
     2821 cattattcaa aaataagatt tttgctggtt caataaaaat acttgtcaat ggaatcaaat
     2881 gggcagataa aatgatctct acaccttttg ccattgcttg agtcaatgca tcgacaactg
     2941 gaccaatcgc agaaaaacct aataacgcta atccaaaacc gataagacct gctgaaaaat
     3001 tattgactag catttcaaaa ccggatttga tccgtttttg gaatagctga tcaaatttct
     3061 tgatggcata tccaccaaga ggtcccatga tcatcgcgcc tagcatcatt ggaatatctg
     3121 ttccgacaat cacacccatc gttgcaattg ccccaacaac tgatccgcgg tcaccagcaa
     3181 ttacttttcc gccagtataa ccgatcaata aaggaatcag ataagttagc attgggctta
     3241 ccatggtagc caaagcctca tttggtaaat aaccatctgg gataaataga gccgttaata
     3301 caccccaggc aatcagtgct ccaatatttg gcatgaccat gcttgataaa gtactgccta
     3361 atttttgtac cctcgctttt agcgaaatgt tgtttttggg aatcgtccgt tccattttgt
     3421 ctctccctct ctccttttga tactctaatt gtatccgctt actaaaaaga gaaatagtcg
     3481 tactttggca cgaaactttg tgccaaaggt ttgagtatgt cagcaaaata aaaagatttc
     3541 ggtatcagaa aacttcaatt ttaaataaaa ctgttatttt aattttttta taatatgcaa
     3601 acaataactt ttgttagcat ttaatttaga tgttttccat tctattatgt tattaaattt
     3661 tatattggag gcctatctat gacattactg atcaaaaaca tgaacatgaa acgtggtaaa
     3721 aaacgtgtga ttcgacaatt gaatctttta gtagaatcag gagaagtagc tgctttgatt
     3781 gccccgaacg gatttggcaa aacaactttg ctgaatggca ttgcccaact gctgccaaca
     3841 acttatgatc aattgtccct tcacaacatc tctcctactg cttatacaga ttatcgccgg
     3901 tctttctttt ttctagaatc aacgaatcag ttatccgact atctatcggc tgttgattat
     3961 ttgactttga tcaaatacta ttggcagagc aaagtagaat gccaaactgt tttccaacga
     4021 ttgaagatga ctgagtacct tactttgccg attggaaaaa tgtctctagg aatgaaacaa
     4081 catgtcttgc ttgctgctta cttgatcagt gattcatctg tcatgttatt cgatgaaccg
     4141 ttgaacggat tagacccagg aagtattgat atcttgaata gcctcatcag acaatggcag
     4201 aaagagggga agacgattct tctttcttcc cacgctatcg cgaatatcca atcgatttgt
     4261 tctaaagcat gttttctaaa aaatggagaa atcgtcacag aatcctcaaa tatggaagag
     4321 attttagcta tttatcgtaa attatatgag gtgtaactat gtctttactt acatttgaac
     4381 gcaatctctt actaaaaaac aaagtaaact ttttatttcc tctattgttg gtcgtgctct
     4441 ttgcattccc actttttttt gatcataaac ttgcttatac agaattcgat gaactgaatc
     4501 ataactatga agagatgcaa cgtttgattg aaacgttgaa agaagatgag aatgagaaag
     4561 aatttgtgga aagtttagag aaaagcaaca aactgatcga agcaatctta catgcaaaaa
     4621 acacagggaa tgtccagcaa atggtcgaag caacttatca ttatgaaaaa gatatactgg
     4681 atcgactgat ttctggtcaa agacaaggaa ttccaatcat tgaacaacaa aaacgtgtcg
     4741 agttgctccg ttatatgaaa gaacatcaga tccaaagata ttctattttt gacttgccag
     4801 cgcatctccc tttagcaaat tactacgaaa atattttttc aggaatgatt tctagttttt
     4861 tgattttatg tataaccgcc ttgtttcttt cttccatcat ctcttatgaa aaaagaaagc
     4921 aggtaatctc tctagtcaat ctattaccgg atagtatggt aaagaaacat agcatccgtt
     4981 ttaccattta ttatggtgca gctatgctaa gtctcgtgat gccatttttg atcgtctcta
     5041 tactagtaat aatcaaaaat ggtttgggag acttccgtta tcctgtagga acgatcattg
     5101 gtcaggagat acgaatcctt ccgatgtatg agtatctttt ccagagcttt ctgttcctgt
     5161 tgttgtgggt gctcttttta agtacaatca gttttcttct atctgcttta tttgaacatt
     5221 ctctagtcaa tcttttaggt acacttcttt gtcttttctt agcagagtat cgcttgtttt
     5281 cttcgattgg atggatagaa tcaatcagtc attacttgcc aacttcttat gttgattttc
     5341 aaaatgtgat cataggagga gatattttct cgccactagc ttcagaacag gttactttca
     5401 tgaatggtat cttgacactt ggtatatgga gtatcgtttt attattcata ggtatgggca
     5461 ctatctatat aaaaaagagc tattaaaaca caaaaataat aggggcggtg acaaaaacct
     5521 tgtcaccgtc ccctatttca aataaacggt tttctagaat aaatagtttt caaaagcttg
     5581 catccgcagt aataagaacc tcccaacaaa aatagctcct cttattttta tcttggttga
     5641 agttattact tgatgcaagg cagctttttt tactgtgaac attggttata tatgtccatg
     5701 ttcaaaaatc atttggactt cttcaatggt tttcgcatca ctgagcgcca ttacttggtc
     5761 gatgtctgaa cagaaaaagg ctaaatcttg gagcaccgct aactgatgtt ctttttttaa
     5821 agccactaca aataagagtg tcgcgatttt tggttcctct tttgttccaa agtccacacc
     5881 atcaggcact tggactaaat agattccttc ttctactact tcttcatctt tgtcagtccc
     5941 atgtggcaag gctacgaaat tcccgatata gacactcgct ttttcttgtc tttcgatcat
     6001 tgattggata tatgccggtt tgattccccc ttgttgggtc atccactggc cgatctcggt
     6061 caacgcttct ttccaatttt gataggattg atcgagcaat attttttctg ctggtagctg
     6121 tatcatcttt tcaccttcat ttctttattt cttcaatcag gattttcgct aacaactgat
     6181 aaacgatttc tttatttcct gagcggaaga tctcggtgtt caagtcattc atgatgattg
     6241 ctccactgat ctttcctaaa agaatcccgt ctttttcttg gattggcgaa ggcgccagca
     6301 taactaacat acgggacaaa tcgactggct gtctatccat cccttcaatg gtcaacggat
     6361 gtttcaaatt gaaaatacaa aagatcggtt tagaaatcaa tgagctggat gtatggaata
     6421 accccatatt tgtatgagga ataccgatag gtgcttgttc atatcgctta agcagccggc
     6481 gatgaaccac ctcttcatcc gttactagtc cttcaggaaa ataaggaaat aagctgcgaa
     6541 tcgtttcgga aatcgtctca ggattatcca gttcttggat aaaaaaacga ccgagtaatt
     6601 gattgatttg atccatgaaa tgcattgttt cctcataagt atcttctatc atccactcgt
     6661 tattgctgtt atacgtaatt ttttgccttt ttttcttctc aatatgcgaa aaagcttctt
     6721 ttagctgctt gacttcatct tctagcagta gcggggagac taaaatatat tttccaccgt
     6781 atcctggtaa aatagatgta gaaataacaa catcatattc ttcttgtaaa tcgatttttt
     6841 ttaaatcagc aatccgatag aaatgaattt gttggatgaa gggaaaatac cttttcaatc
     6901 gtagttccaa catgctagta gaagctaatc cacttggcga aaatcctgca atactgatat
     6961 tgctttcttt cggacttcgt tccaatgaat tcgcaaaatg aaggaccata taagcgatct
     7021 cttcttcgga taattttttt tcttgaaaaa tccgctctac tgctttacgg attgcattcg
     7081 caacttcctc gtattgaacc atgatttttt ctaaaatcgg attggtcagt gtctcttctt
     7141 gtaaaacggc tcttgaaaac acaccagata aatgagtcaa cagcattttg tacaaagttt
     7201 catcttcata aaaatggaca ttcgtctctt cacttacttc ttgaatcagt tgttttacta
     7261 aataagcaag ctgtgtatcg aacgtctctt caaagaaatt ctgatcaaat gagttcgtga
     7321 agtcatttaa taatcctgca aaaaagacga tctcatttac tggataaagt tgttttgtct
     7381 cagaagcgat ccgtgaaaac aatcgtttag ctgtctgtag taattctttg tttgccagac
     7441 tattatatgt ctcatttgtc agtaaatgat ttttagtcac acgctccatc gtcaatgtca
     7501 gcatcaaaac caaatgttca agctttctat cacttagatt ggtctttttt tggatcaatt
     7561 cttccccgat tactttgcgt gcaagagata gatgttgcgg atcaacgaat cgaaaaaaat
     7621 aattcagtga tgcatcgaaa gaaatactat gaaaaagttc atattcattg atttctaatt
     7681 ctaatacatt cgctgtcaga agccggcggt atttttccgg tccttcgatc tcgtatccct
     7741 ggttccgaat gatttccaaa ggcagttggc gaatactttc ttccaactgt ttgatatctg
     7801 cataaaaagt ggtattactg atgcgatatg attccatgaa atgagcaaga ctcaacggct
     7861 ctgtcgtcag taacaactcg gctaaaatcg cgtgttgtcg tttagcagtg gataagtcat
     7921 tacttttccc atctgtattt tcttttaatt gctctaatac ttcttctgat gcctcaatga
     7981 caaatgagcc tctagctgct tttttcaaag aagcatttac cgatttcaat gagacttcaa
     8041 gattatctaa ttcgcggtaa atagtccgtt tactgacctt caataaattc atcatttttt
     8101 ggatagatac aggtgacggg ctactgagaa attctgctaa taattgtttt tctctgggcg
     8161 ataaatacat cttatatttc tcccttcacg agtttttcgt attcattcgt agctacccaa
     8221 tgttcaacta gaactagccg agggatcttt tcttctagct gttcagttac agctagttct
     8281 ctttgtgcga tataaatcgt ttcttcttgg attttgattt tttctattga ttcttttgag
     8341 ataacaaact gttttttcgc tttttttgct aattgactta aaatcgtcat gcccatagtt
     8401 tgtgagccgc gtctgttgtt tttatacaaa aatacaatat tagcaggcga ctttttttgt
     8461 tctgtgatat ctgctatctc ttttttttct tcccgctttg gaaattggat cgcttctttt
     8521 attcgtgaca gcaagatatc tgtttccaaa aagtgatcca gtacatagat ttgactttga
     8581 ggtacttttt ctcttgcaag acccgctagt tcatgttgca cgataaccag ctgatttact
     8641 tggttattca attcatagat agagctgtat ccgacagaaa gatcgatttt ttctgctgct
     8701 aatttctttt tcagcagggc ggctcccata gcacttgatc ccatacctga gtcgcaagca
     8761 aaaatgacgg aatgaatgat agatggttcc aagatttctc ccctttcttt gaccgttttg
     8821 ttctcttttt ctttatttgg taatttcaat agtatcgctg cacaaatgaa ggtcacaaat
     8881 gtactgattg ctatccctat acttacccca gctagttgtg gataaggcgc attggcaaaa
     8941 atcacgagta gtgacccagg cgatacaggt gcagctaatc ccgtatctaa ccattgaaat
     9001 atcaacgtgc cgcttgcccc accagccatt acagctaaaa atagatacgg tcgcagcaag
     9061 acaaatggaa aatagatttc gtgaatcccg ccaatcgcat ggatcatcaa tgctccactc
     9121 gcatttactt tttcctgttt ttctgcaaaa caaaggatag aaagtaagat ccctaaacca
     9181 ggtccaggat ttgcctccat caaaaataag gaagagtacc cgactgtttg tgagaattcg
     9241 attcctaaag gtgtcaatac accatgattg attgcattat tgaaaaatag tacttttaga
     9301 ggttctaaga tcagactgac aagtggtaac agattcattg tgactagcca agacacaacg
     9361 gaggcgagca acaagtgaac actatttaaa actggttcaa taaaaacgat tccaattata
     9421 caaataacgc taccagtcag tcctactagt aggttgctga caagcatttc atatccagcc
     9481 ttgaatttca cgtggaatct agaaacaaat tgttcatata cgatgccggc aagcggcccg
     9541 ataatcatcg ctcctacgat ttgtggagca ttggaacgag cgatcaaccc taaagaagca
     9601 attgcaccgg cgactgcccc tttttgtccc cctaccgatt tcccgcctga ataactgatc
     9661 aagatgggta aaagcagctg gatcatccaa tggcatacct cttctaatgt ggtgtgcaaa
     9721 tcgccagaca caccttttgt tgctaatgac aagattcccc aagcgataaa cagacttaga
     9781 tttggcatga tcatttcatt taagtgatgt ccagcttttc tcagttgttt catagacacg
     9841 cgactgctgt tgtttgcttt ccatttgtat tccatctttt tcaccatact tctatttatt
     9901 gatggacgga ttttatgtag aaaaagcagc aaacgaaaaa ccgtctgctg ctttcagcta
     9961 tctgggaact atttgcctgg ttcagtcgca atgattgcta gttcaccttg gaccgcccac
    10021 tcagaaacgc catttggaat aatacagctt gttccttttt ctaatggata tgtttttccg
    10081 tcaacgacaa ggtctcctgc tccttcgatt accgtcatca atgtataagg ggcttgtttt
    10141 ttgaaagatg tgattccttt aatatcccac tcatatacat tgaaaaactc agtttctaaa
    10201 taagtgacga tcgatgaatt cccttttcgg acttctttga tttgtaattc tggtgttttc
    10261 gcagggacag ttgttacatc gatcgattgt tggatatgca gctctcttgt ttgtccttgc
    10321 gcatcttttc tgtcgtagtc atacacgcgg taagtcgtgt cagaactttg ctgtgtttct
    10381 aaaatcatga ttcctttacc aatagcatgg atcgttccgc ttggtacata aaagaaatca
    10441 ccttttttaa ctggtacttt cttcaaaagg tcatcccagc gcccatcttt gatcatctca
    10501 gctagttctt ctcttgtctt tgcatgatga ccatagatga tttctgcacc aggttcagca
    10561 tcgatgatat accagcattc agtttttcct aattcacctt catgtttcat cccataggca
    10621 tcatctggat gtacttgtac agatagatca tcttctgcgt ctaagatctt gatcaataat
    10681 ggaaacactg gttctgaagg atggccaaac aattccttgt gttctgacca tagttcatct
    10741 aatcttttcc ctttgaattc gccattttct actacactga caccatgagg atgcgcactg
    10801 atcgcccaat cttctccgat tttatcgtta gggatatcaa aaccaaagac tgaacgtaaa
    10861 cgacttcctc cccaaatttt ttcttgaaaa actggtttta aaaacatagg ttcctgcata
    10921 attcaaactc ctctcatttt tcatagctat tatagcatta tttcggtaat accgttttct
    10981 ctttcaaaaa gttttcaaca atttcatctc gacatatgag atactccgca cgatgatcga
    11041 caggatgtac gcgatttgac aaaaatatca acgcttcttg tgtttttata tcaatcaaga
    11101 aaaacgtacc tgtataacca gtatgaaaaa gaatcgcatg accgtcttct acatcctttt
    11161 ttaagtccca tcctaaagaa cgatagccat tttttttcgg tgtttgatct tgcagtaaag
    11221 aatcaatcgt ctcttcttct aaaaaacgac cattacggat ttttcccttg ttcaaataca
    11281 tttggacaaa agtaatgctg tctttcaaat caataaagag acccgcgtta cctgcatgtt
    11341 cagccaaaac tcttgctttc ggatcatgag tttgtccacg caaaacctca cccgaaggca
    11401 gttgttctgt gggaacgatt tttttggaag gaaaaggaag aaataagctg ttcatcattc
    11461 ctaatggttc caatacttgt tcttgaaaga tttccactgc agtttcttgg taaatttcct
    11521 ctagcatcaa cccaagaaga atcgtaccgg catctgtata ttttacttct tttcctagtt
    11581 gctcacctgg ttctagcttc aaataagcgg cgatcaattc ttcctgagat agcttatctc
    11641 tgtttggtat ccatgttttg atatcagaag tatgtgtcag taaatgacgc aaagtgattg
    11701 tctgattttc aaattcagga agatatttat gaagcggttg atcgagttct atctttccat
    11761 cttctaatag acgtaaaact aatgaagtgg ttgcaacgac tttcgtcagt gaggctgcat
    11821 caaacaacag atcttctgtc atcagctgct tttcaggaac gacttgggca aagccggctg
    11881 taaatgtttc tacttgattt tcttttatga aggcgactgc tacacctgga taaaatcctt
    11941 cttccatctt tttcaaaata agctgtttcg tgtgtggata catctttttc tcctgctttt
    12001 ctatcagatt cttacagtcc aagcttaaag cttaatctat gaggaatcaa ccttttttac
    12061 acaaaaaaac tgcgcgaaaa accgcgcagt ttcccccggt atactatttt ctctttctat
    12121 ctctagaata agcgatagta tgaccgttca taaaatgacg agacatgcga caatcgttaa
    12181 gaaagaaaaa gttaagcgtc gcatcgaatg tcagttttat tcttttcttt tggctcactg
    12241 acgaaccacc attcgattcc tatagcatca tagattgtga gtactgtgtg ctttttgtct
    12301 aagaagaaat caactgatag ttgctgcaag tgagcaatca gtccttccat gctttcagta
    12361 tcgatcatga atttcagaaa atcaagtcca atcacttcat ctgtaggcaa atcaattgcg
    12421 cctccgctag cttctgtcaa accaacatgg aaatctcctt cattcaatac atggatgccc
    12481 tcttcttcat tatgtacctt caaaccaagt acatgattaa gaaattggcg ttcttttgtt
    12541 aagttagtaa catttaaatg gattttttca aaataagaac ctgttgatag tttactgaat
    12601 ttttcttcag attttcttaa taatgcttct tgatccattg tttctggaat ttctctttgg
    12661 tcgttcttat tctcttcatg gtaaatttcc aattcattct cttctgggtc tacgagcaaa
    12721 atccccatac gtccatgatc ttctaaagct tctttgattg gataattcat tttacggata
    12781 cgacaaagga tatctgccat ttcttctgtg ctagggataa tcaatgaaaa acggttcagc
    12841 tttttgatca cccccatgtg atcattcgct ctaggacttt cttccagcag caacatttca
    12901 cttccagctt cttctgttcc taaaatggct aattcgtttt cttctctttt caaatcaaag
    12961 ccaacaacgt tcttgtaaaa atcaatcatt ttatctcggt ctttcacccg aatggctaca
    13021 ctttttaaat aggtggcagc ccccaattga aaatctttca tatttcttgc tcccctctac
    13081 gtcaagcttt attttaacga tggattcttc ttctttcaag caataacttt gattttgtta
    13141 aaaaaaatgt gagaaaattc gcattttgct tgttttttgt aaattaatga gtaaaaactc
    13201 ttataaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa
    13261 aatagctcct cctatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
    13321 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gatacaaaac
    13381 ggaaaaatag cttctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg
    13441 aacaccgttt attcggaaaa agggactgtg acacgacttt tgtcacagcc cctaaaaaaa
    13501 ataataaatt tgtactattc tatgaagctt cttcttcaga aaactgactg ttataaagat
    13561 ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt tcctgtttct acgatcgaac
    13621 catgattcat cacaataatg ttgtctgcat cgcgtattgt agacaagcgg tgagcaacaa
    13681 cgaaacttgt ccggttttcc aataggcggt tcatcgcttt ttgaatcagt atttctgttc
    13741 gtgtatctac actagatgtt gcttcatcaa ggatcaggac atctggattt gctaagaatg
    13801 cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat attgcttgca tcctcgttta
    13861 atacagtgtt atatccttct ggcaatttac gaacaaaatc gtctacgtga gcagctttag
    13921 cagcctcaat gatttgttcg tctgttgcat cgtcgttccc atatttgata ttgtcataga
    13981 tacttcctgt aaacagccaa gtatcttgca gtaccattga aaactgtgcg cgcaattcat
    14041 cacgtgaaag atcacgggta tctacaccat cgtaacgaat actacctcca gaaacatcgt
    14101 agaaccgttc aagaaggttg atcagtgtcg tcttccctgc accagtcgga cctacaatag
    14161 cgaccatctc accaggtttg acatccaagt taaaatcagt cattagcaag ttatctggtg
    14221 tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt tcttctggaa
    14281 ggccagattt ttcattggac atctcttctt catccaacac ttcaaatacc cgttcggctg
    14341 aagcaactgt tgattggata gtgttcatca aacttgcaag ttgagtgatt ggttgagaga
    14401 actgtgttgt atattgcaag aacgcttgaa catcccctaa tgtcatgttc ccatttgcaa
    14461 cttttactcc gcctagtaca gcgacgaaca catagcctag atttttaacg aaattcatca
    14521 acggcatgat gattgccgaa ataaattgag ctttccaacc agcggcatac agattctgat
    14581 tttctttttc aaatacttcc tggtcttttt cagtatgatt gaatgtttta acaatggtat
    14641 gaccaccata tgtttcttct acttggttat tcaataaacc taaacttttt tgctgatctg
    14701 cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat gatcaagctt aaagggatca
    14761 tcaataacgc gactaacgtc aattgccagc tgattgaaag catcatcacg atgaccccaa
    14821 tcaatgtgac tgcactagtt acaaattgag tcaaattttg ctgcagcgtg cttgcgatgt
    14881 tatccatatc attgattgct cgagacatga tatccccatt tgtatgtgta tcataatagg
    14941 aaatcggtac acgattcatt ttggcttcca aatcatgacg taattcataa actgttcgtt
    15001 gggaaacacg tgtcataata aattgctgta aaaagttgaa tacagcagaa atcaaataca
    15061 tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga aattttgtta
    15121 cttgttggcc ttgttgcatc tgttgagcgc cagccattac acctttgaat atttctgtgg
    15181 ttgctttccc caagaccttt ggtgtttgga tttggaagat cacggctgct attgctagta
    15241 caaatacagc gatgatcgca accatacgtt tagacatata gccaaacaat cggcgtaccg
    15301 ttccccagaa atttttaggc ttttcacctt ttacaccgag atttctgcca ggtccaccat
    15361 gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt tgttttactc atgctaagtc
    15421 ctcctctctt agttgtgaat gaacgatttc ttgataagtt tcgtttgttg ctaatagttc
    15481 ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc acaaggatca tatctgcatc
    15541 cattactgta cttactcgtt gagcaactaa aacggtgata ctttctttca tattcgtttt
    15601 caatgcttgg cgaagattgg catccgtttt gaaatccaac gctgagaagg aatcatcaaa
    15661 cacatagata tctgctgttt tgactaatgc ccgtgcaatt gccagacgct gtctttgtcc
    15721 accagagaag tttcctcctc cttgttccac atggctatct aagccatctt ccatctctga
    15781 aacaaaatct ttagcttgtg cgatttctaa tgctttccaa atcatttcat ctgttgcatc
    15841 cggcttacca tactgcatat tgtctcggat cgttccagag aacagaactg ctttttgagg
    15901 aacaaagccc attaattctc gcaaattata ttgagtcatg tcactaatgt ccgttccatt
    15961 tatttggatc gaaccagatt cgatgtcata taatcgaggg atcaaattga ctaaggtcgt
    16021 tttacctgaa ccagtccccc ctatgattgc gacgatctcg cccgacttgg cttggaaatc
    16081 aatatcttcc aatgcaagtt tttctgctcc atgatatcga taattgacat gattgaaggc
    16141 caatgttgcg ttttttccag caaaagaaac tgttttagga ttttcagggt catggatacc
    16201 gattttttca tccaacactt cattaatacg gtcagctgaa gcttgtgcac gaggcaccat
    16261 gataaaaata gcggaaagca tcataaagct gatcaagatc tgcatcgcat aagtcataaa
    16321 tgcaatcaga ttaccgactt ccaaagtcat atctgctatg tagtgtccac caaaccaagt
    16381 gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca atgcgacgat
    16441 cgtattaact ttgattgccg tatttgtata atctttattc gctaaatcaa aacgattttc
    16501 ttcaaagcgt gtcttatcga atgctcgaat cactctgacc cctgtcaatc cttctcgaaa
    16561 taccaggttt aaacggtcag ttttcttttg catacttttg aataatggaa cagcaaagta
    16621 catgattccg ccgatcaaaa taatcattac tgggatgaca actaagaaga tttttgtcaa
    16681 ctgatgatct ttttgataag caagtatgct cgcgccaatc aacgtgattg gtgcattgat
    16741 catcatacgt aaaaacattt gtgtaaccat ttggatttga gtcacatcat tggtcgttct
    16801 tgtgatcaaa gaggccgttc catatttatc aaacgcatct tttgtcagac tctctgattt
    16861 tttatagaca tccgtacgca attgcttacc cagtttctgt gattcacgag tagcaaaaaa
    16921 tgtatttgcg atagcagcta agatactgat caaagaaaag ccgatcatca caaaacctgt
    16981 atgccaaata taatcgacat ctccttgcgc taccccttta tcaataatat tggaggtaag
    17041 tgtcggcaaa tataaatctg ccagtacttg gatcaccatg aaaactgcag ctgcaattgc
    17101 tgaagtcaag gagatccgtt ttacgatttt tagcatctag ttcccttctt tctatttata
    17161 tactccgttt ttcagccaag aaagtttcaa attgaattca ctgattgctt gctctactga
    17221 gtaatcttca gatgttgaaa ataagcgata cgaatgcgca attgttgtaa atacagtatt
    17281 catcactaat tggatcagta attccaattc atgatcattt tttactttca gcttcgtccg
    17341 atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg gatcgtgagg
    17401 cgttttttgg cgactcgtat gaggagcgac ttttcttgag gcacggtaat ccatgttcat
    17461 aaacaagtta cgataaaaag aagcattctc gccttccaaa atctcataaa tcattttaga
    17521 aaaataggat tcgaatcctt tgaatagatc accatcggat tctttcatta gttgtaacat
    17581 atctttagag ctgtcacgtc tcattgtctc aaagtaataa aaataaagat cctctttatc
    17641 ttcaaaatac tgatagaaac tgcctctagg gatatccgct aatttgacga tattagcaat
    17701 cgaagcttct tttaagggta ctctagaaaa ctcgattctc gcagcctcga ttagtctttt
    17761 ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc atatggatcc tcctttcttt
    17821 caagatgaca cattgtcata tgacgctgtg tcattataac acctcctacc tgttttgcaa
    17881 gacatttgca tagttttttt cattatcatc cgatacatta aaaaagtgat aagaggaatc
    17941 cttccatttg aattaatgag agttgcccat taaaatagcc acatactaaa caaaggatga
    18001 gataaatggc ccaatcaatt agtgtccaag acttcaataa attgcctctc gatgctgaca
    18061 gcatcgtttt agatacaaga aataccttag attacacaaa aaaccactta agccactcgc
    18121 tttctatccc cgctgctatc ttgccaagac aattaaaaaa tctagatcca aagaaacttt
    18181 attatattct aagctataca ggcaggcgat cagaagcatt agccgatcaa cttactgcta
    18241 atggttttcg tgctgtttat atcattggtg ggatggaagc tcttcagtcg agtgttgctt
    18301 gaatattgct tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac agcctctttt
    18361 ataccctgtc tttttttagt tatttattct tcaaccttat caatatgact tgatagcctt
    18421 ttctttttct catagttata acaatcatag caataaaagg agaaggtaag catatggcat
    18481 ctgttttagt agtcaaaggt catcccctaa ctgcagaaga atcgcgcaca gttaaggcat
    18541 tgacgtcttt tttaacaagt tataaagaaa atcatccaga tgatgaagtg actgttttgg
    18601 aactttatcg cgatgatatt ccggaaatcg atgaagaatt attatcaggt tgggaagcat
    18661 tacgtgctgg tgcagaattc acttccttat cagaaagtca acaacaaaaa attgctcgtt
    18721 tcaatgaatt gactgatcag ttccttgctg ctgataaaat cgttatcgca aacgccttat
    18781 ggaatctaaa tatcccaaca aaattaaaag cttggttcga taccgtcaat gtagctggaa
    18841 aaacgttccg ctatacagaa aacgggccag aaggattagt gacaggtaaa aaagcactgc
    18901 acatccaatc taatggcggt gtatacaacg gacaagactt tgcctctcaa tatgtaaaag
    18961 gtattttaaa ctttgtaggc atcgatcaag tagatcagct attcatcgaa ggaatcgatt
    19021 atgaccctga ccgtgcagat gagttgatgc aaaatgcatt agataaagct gctgctttag
    19081 gtaaatcatt ttaaaaattg agataaatgc atacaaaaga gacagaggaa aaaatcttca
    19141 aagcagcatt tgtaccttgg atattttccc atctgtctct ttttgttatt ctttctgctt
    19201 tttcaacgga ttttcttcat aaagagaaga gattgttgtg taccattcaa aaacatgagt
    19261 aatcactact ttagctgctg cataaacagg aatccccaag atcacaccga tgattccgaa
    19321 caatttccca gaagtcagca gaactaataa aatcgttact ggatggattg ctaactgatt
    19381 ccctagtact aatggagaga tcaagcgtcc ttctattgtc tgctctactg caaaaacgat
    19441 cagtactttg atgatcatga atggacctgc tacaatggct aaaaagatag ccggaatcat
    19501 cgctaaaaat gatcctaagt aagggatcag gttcaaaaaa cccgcaataa tccccaacgt
    19561 gacagcatac tctaaaccaa tgaccgcaaa accaatcata aacatgattg ccacagcaaa
    19621 agccacagtt agttgtcctc ttatataaga agaaacttga ctattcattt cactcagcac
    19681 attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg acaaaataag gtgctaattg
    19741 tttaccgtct ttcaatagat aaaataagat aaacggcatc gtcagcagag ccactaaaat
    19801 agtcgctacc gcgccgacaa aacttccaag actctgaact gtactcttag atatatcttg
    19861 gatcatatcg cctgccgaat tcatgaactt atcacctgct gtttccaact gttcgcgaaa
    19921 ttggttgaac agcgggtccc gcaaaatctc ggttaattta ttatcaatcg tatctacata
    19981 ttgagggaag ttactgatga agctcactgt ttgttcctga atcttaggga tgatcactac
    20041 gctcccccat gcgattagtg caacaaccaa aacaaacagc ccaatgatac tgtaaagcct
    20101 tggtactttt cgcttttcaa agtaatcaac taccggattc atcaagtaat acaagattcc
    20161 tgctaaaatg ataggcagac ctacgattgc taaaaattgc cagattggtg caaacaaata
    20221 tgaaactttt gtaaataaaa agacgatcag caatattaac aatacaacaa gtaaggctgt
    20281 gactacttga ttatttaaaa accagcgcca aaaccacgat accggtctgt tcttcttatt
    20341 ttttccttcc ataattcctc ctgttagtta taaaaaattt ggcttcccaa accaatagca
    20401 ggcagtctat gcggatgaag atagattcca tttgcaattt gtccttcttc tggacaagca
    20461 tcaaaaatca atgatacgtg agctactgtc tctaaattcc cattggccat cggaccgaca
    20521 taatcgatcg tatatacctg atcatcaaaa gcaattgttc cgcctgcttt caaagaaaaa
    20581 ggttcgtctt ttgatacttc ttgaatcacc gaatattttc gtaaggcagc tgttgctgtt
    20641 ttatcgaaca aaatcagcat gggttcttcc ttgtttattg cttctgtccc aatttctgta
    20701 atctttgcct gaatcattgc actctctcct ttaattgctt tcaaaaacag tataacacat
    20761 tctcattctt tagccgtgtg gaataattat gtgatagaat aaaaacgaaa actatttcat
    20821 tacaattagg aggaatcagc tcatatgatc gaaaagataa ttttaggaac ctatacgcgt
    20881 cgcgaaagta aaggaatcta tacgatcgac cttgatacag aaaaagaaga attagccaac
    20941 ctttcattag ctgttgaaga aaatagccct acctatgtag caaaaagtaa agctggcaat
    21001 ctttacacag ttactagtgt ggatggatta ggtggtgccg gtgcgtacga taaggatttt
    21061 cattttttga atgcggtaac agagtctggc gctcctttgt gttatgtagc ggttgatgaa
    21121 gcacgtcaat tactatacgg ggcgaactat cataaaggtg aagtaaatgt ctaccatata
    21181 ttaggagatg gcgaactgaa agcagcagat tcaatattcc atcaagaagc gacaggtcct
    21241 cataaaaacc aagatcatgc gcatgtccac tatacggatc tgactcctga ccaacgatta
    21301 gtcgtatgtg atcttggtac agatcgtgtg tatacttatg atgtgtccga aaacggcaaa
    21361 ttgaatcttg taactacctt cactgcagag ccaggaacag gccctcgcca tcttgtcttt
    21421 catccaaatg gacaattcgc ttatttattt ggtgaattag acagcacagt ccgcgtgctt
    21481 tcttataata aagaaacagg tgcttttgaa gaattacaga aaatatcgac cttgccagaa
    21541 gaattcgacg gtgaaaatgg aggggcagct atccgcattt caaatgatgg caagttcctt
    21601 tatgcttcta accgaggaca taactcgatt gctgtattcg cagtttttga agatggttcc
    21661 cacattcaaa acaagcaaat catctcaaca gaaggggatt ttccccgcga tttcgcacta
    21721 gatccaagca ataattttgt cgtgtgtgcc aatcagaaca ccgataatct gacattatat
    21781 cgccgtcatc ctgaaacagg attactggaa ttactgaaaa aagatgtcta tgtaccagaa
    21841 tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga atgaaatgaa tcagcaagac
    21901 gtattagaca gactacggga agaactaaat attcctttct ttgatgggaa aatcgaagat
    21961 aaagagtata gcgaagagga atatcagaaa ctcaaagccg atttacagaa ctactttgaa
    22021 caatatgtac agaatgtaga aaactaatca gctttccata ctatctgaaa agaacatcag
    22081 gacaagactt ttccgtctat cctgatgttc ttttctacaa ataaaaaacc gccgagatgg
    22141 ttagactcga cggaaaagga gtatttactc gattttgagt aagtacaagg ttgttgttgg
    22201 ttataggtat gtcattatca taaaggattt tgatcactct atcataaagg taatattaca
    22261 tttatattag ataactctag tttttattgt tatcaaagat ttcttttatt ttttcaacaa
    22321 caaattagtc attttttgga tggcagctgt caattggtct gtcgcttggt cttgttttgc
    22381 ttcagatgta ttgaatagat cttcatctac ccctgattca agatcgatac tttggatcat
    22441 ttcttcacag cctgctacat atgaaagata agctttttca aaatttttat gaatgcccat
    22501 tgcttttgct ggtggacgca aagtactgac tttcttaagc attgcccgat atttttttgt
    22561 tccttcttca aataacagga tgatctcttc aagagtcgat ttgtccagtt cacttgtttt
    22621 tccttcatca attgcgtttc tgatcacttc atatttcgga tgcattcctt ctccgattgc
    22681 ttctgtttcc ttgacgattt gatcgatgga ctgcacataa tagccaatat tcggtaacat
    22741 gttcgttttc ctactttctc cattgttgtt attatttatt ctacttgttt ttatcctcaa
    22801 atgacaagta cttgaaaaaa tttaatggtt tctatacagc tcgccattct tattcgtgct
    22861 caaagaaacc atctgctaaa ctatattgtt tccctgcgac aatgtcatat tggatcaatt
    22921 gatattcgtc gtaaatctct tgttgcgagc gattgaattg cgcttcttta taccactgtc
    22981 cgttttgata gtacagttct cgttcaaacg ccggaagttc ctgttctaat gataaaagca
    23041 gctgataaaa gccatttgtt gtctgattcg tctgttccat tagattcgct gcaaaataaa
    23101 acggactggt gattgcatcg tgagtcgttt gcttttctaa ttctccttta ctgtcgaaca
    23161 taagaaattg tgtttcatgc aatgtttgtt tgtcattttt tgcttggatc gtatctgaat
    23221 agatccctgg aagatgatcc ccccaaaaaa caactaaagt ccttctagaa agattcttca
    23281 attcttccgt gaactgcttc agtgccgtgc tgctatagga aatatcttgc agataattct
    23341 ctaagtcaag tgtcccactt ccatctgaag tttttgcagg atagtctaga ttatcatact
    23401 ttccatcgta gggcatatgt gtctgcatcg tgactaaatg aatgaactga ggtgtttttt
    23461 cttccttgag aagtgtcatt acttctttgt aagctgactc atcagaaata tatggattat
    23521 tctctatcgt atccgtatag gtcatcgtac gctcgctgat gaactggtca aatcctaatg
    23581 tctgataaac atcctctctt ttatacatcg aagtattgta tggatggatc gctgtcgtct
    23641 ggtatgattg tgcgtttaac gctgaaacga tagaaggaag ctgatccatt tttggtacaa
    23701 gcatcgtgta aggggtggtg agttgcgcat taaataacgc catagaaaaa ccagtcaatg
    23761 cttcaaattc gatatttgct gttccaccgc catagttttg agaaagcata ttgccactat
    23821 atgtttgatc agctacttca taataatctg ccaatggctc accagatact tctacgccgt
    23881 ttaatcgact aggatctgag aaactttcac tcatcacgaa cacgatgttc ggcgactctt
    23941 cctctacagt tttatttttt tcatcggcta gcttctggta tttttcagtg atttctttga
    24001 tcttttcttt cgaatatcct tcaggttcat ccattggttc taccttcaga ttgtacaaaa
    24061 acccaccgat aaaaccagtg ttatagtagt tcattttctg gctgtaaggt atccatagtg
    24121 ccgttttatt atatgctttt cgcaacaaat tatcgggatt gttgaaatta ctgatatacc
    24181 ctagcagtcc aattgccaca agcagtgtcg ttaatcggat caattggaat cgccttcctt
    24241 ttttcaggct tcgaaacatg taccagcaaa acaatcctat cacggttcct gcggcagcta
    24301 agatcactgt aaacagcata gtgccggtca tatctttcag caatccgatt tcagtgatca
    24361 tcttcaaatc gtctggataa atcggttctt gccgataact cattttcata taatttgcaa
    24421 aacctaatac accgatggct acgacatacg taagggaacc taaagggaca gatcctgcta
    24481 gtgcaatcag aaaaataaga ataatcagca agacgagaca ggctaagaag aatttttccg
    24541 tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga tagatcattt tgacaccact
    24601 gcaaaaataa attactaaaa acaacaagaa aaaagatact gatgattgct gctgttcctt
    24661 ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata tcttttcaaa tgcttgctcc
    24721 ttcatttcta tcaataaaag aatgttgctt ctcttatttt agttgtacga aattcgaaag
    24781 taaagaacgg aaagaagaat taatgaaatc caaatattct ctttctttat ggcctttctg
    24841 gtttctttaa taagcggacc tttcgtactc cagaaatttt ttctagctgt tgtttcactt
    24901 gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa tatgtagaca tgctcattct
    24961 tatggttacg cgatatatct gcgatcgcta gctcgttctc attcagcaga gaaaatatct
    25021 ctgctaatat attggttttc ttttgataaa atatagtgaa tcgaaacggc gcttggaaaa
    25081 acattcgtgc ggatggataa ttgaccgctt ctctaaccgt accgaacaaa agataatctt
    25141 ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc ttcctgagtc gatcccccta
    25201 gatgaggcaa cataagtata cggtcatttt ctaagaactc ttcttctgga aaatcagtca
    25261 agtatttagc cagatgttgt ttttccaatg cttgcataac agcatattta tcaacaattt
    25321 cacttcttcc tacattgata agtacagcat cttttttcat tttttctata ttcgcctgat
    25381 cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac tacgatgtaa tccgaggtag
    25441 aaagaactgt ttctaaatct gcttgccgaa cataatccaa ctgggcatcg cggatagaat
    25501 aacccaagac atccatccct aagctatagc aacttagcgc tacttttgtg cctatcgctc
    25561 caagaccaag aagaccgatg gttttgcctt gcaattctcg tcccacataa gcacttcttt
    25621 tgttttctgc ctgttctaaa atattaggac ctgttagtgt ttgtaccata cgacttgctt
    25681 cgattatcgg acgggaagat aagagcaaac agcacaaaac aagctctttt actgcattag
    25741 catttacacc tggtgtattc atgacgatcg tcccgttttc agaagctttt tcaacgttga
    25801 tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa atctggtgta agccattcat
    25861 ctttcacttt cgtactacga ataactattc cttcggctgg caaatcatct attattttga
    25921 aatattcccc ttgtaacttt tctatctctt gttctggaat agcatcaatc aaatgaacgt
    25981 gatacataaa accccctcct tactaacaag tatctcgaaa tttctgcaat tagacaactg
    26041 tttaccattt gacttcctag aagcaaaaag agcagttaga gcagagtagg ctgagtgata
    26101 aaaataaggc tatgacaagt tactgccata agccttattt tcgagagcaa aatattgagg
    26161 gtgagatatc ttgataaatg gtgttccgtt agctgacatt gataataaga ctaaatttca
    26221 aaaaagttga aaagcaaatt tcgtcaatct cttcttattt cttaaaacta agtggctttt
    26281 tcacaacctc tttttatcta ataaccactg tttccaactc aactaatttt gcccacgttt
    26341 caatttgatc tgttgttaga tttagcgaga caacggtatg atgaccgcca cctgcctgaa
    26401 tccatgaatg gacaccttca tggaaattcg gtttagtttt ccataacaca cgtgcaacag
    26461 gtaaattagg tgcttcagtc gttggttcaa aggcttctac ttcattgatc agcagtttat
    26521 aatgcgtacc gaaatctgcc atcgatacga ctaccccttc tcctgctttc ccatcaaata
    26581 caagacgcgc cggatcttct ctatctccca ttgataaagg agaaacaacg attcttggtt
    26641 tcgtcgcagc aagcgtcgga tctacttcca tcatatggga ttccaagatt gcttcttggc
    26701 cactagctag ctcgtaagta taatcttcca tgaatcctgt atctttccct cgagccatga
    26761 ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc ttccccagca aaaccgtatc
    26821 cttctgccat caaacgttgt acagctagtc caggtagttg tttcattcca tacaggtctt
    26881 caaaattagt cgtaaaagcg ttatagcctc cttcttctaa gaaacggcga ataccgattt
    26941 cctgctgagc ttgtactttt acatgttcct cccatgtctt ttcttcataa tcaccatagt
    27001 cgaaggtata caattccttg tactcttcaa ataattgatc gacttcttcg tctgatacgc
    27061 gatcgattac ttgtacaaga tctccgatac caaaataatc aacagtccaa ccaaattgaa
    27121 tctgtgcttc cactttgtct ccttctgtga cacccacgtt tctcatgttg tcaccaaaac
    27181 gagccacttt tattccaaaa ctttcattat aggctactgc aacatccatc caatcagcaa
    27241 tctctttttg gatctcaggt cgtttccagt agccaacgac gattttattt tgtttattta
    27301 atcgagcatt gatgaaccca tattcgcggt caccatgagc ggattgattt aaattcatga
    27361 aatccatatc gatcgttttc caaggaatgc tttcgttata ttgcgtagct aaatgaagca
    27421 atggtttttg tagaagcttt gtcccgcgga tccacatttt tgctggtgag aaagtatgca
    27481 tccaagtgat cacacctgct acttcttctc gataattgac ctctttcatg atttttgtga
    27541 tcgtatcagc agtaactgct aattcttgaa ggcggattgg ataaggcaat tggccatttt
    27601 cattcagttc atcgaccatt tcttgtgcat gtttctttac ttctcgcagt gcttcttccc
    27661 catataaatg ctgtgatcct acaacaaacc aaaattcttt ttctccaata ttcaacataa
    27721 ttagccactc cttttatttg ttttgtccgt aatacgcatt ttttccgtgt tttcgtaaat
    27781 aatgtttatc taaaatccgt tgaggcagct cttctgaaaa tgaattgagc tgtctggtaa
    27841 ataaattcat cttgcatact tcatctagta cgactgcgtt cattaccgca ctttgcgcat
    27901 ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac ccctggaatt gctaagggat
    27961 caattttgcg ttctttgaat gtttctatga taacttttcc tgtttcatat tcgtacccgc
    28021 tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac cgatccataa aaagtatctg
    28081 catgcgtcgt ccccatcgca ggaacatcca atcctgcttg tgcccagatc gtcgcccatg
    28141 tcgagtgagt atggacgatt cctccgattt gaggaaatga acgatataaa acagcatgag
    28201 taggcgtatc cgaagaggga ttgagtttac cttcaatgat atgattgtcc aagtccacaa
    28261 cgaccatgtc gtctgctgtc atctcttcat aactgactcc gctaggtttg ataacgaaat
    28321 atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt atatttgacg agtccgtgtt
    28381 taggcaagtc aagatttgct tggtatactt cttctttcag ttgttccagc attttctatc
    28441 actccttttt cttgcaattg atgaatcgct gttttttcaa tgtcgatccc ctcacggtaa
    28501 cgtctcataa atatctgata gcctgctatt tcttctgctg aaggtgtgat ggtgattcct
    28561 tggtcttgtc caaacacgtc ttctgccaag aagttttcta aagacttccc atgtcgatcc
    28621 atcatatagg ctgctaataa cgcgattccc cacgctccac cttctccggc tgtgtccatc
    28681 actgtaactg gtgcctccat agcagatgct agaatccgct gtccaacttc tggtgtttta
    28741 aaaatccccc catgccccac taaacgatca atttggacat tctcagattt caatatatcc
    28801 attccgattc tcattgcacc aaaagcactg gctaaatgga tccgcatgaa attcgctaaa
    28861 tcaaaacggc tacctggtgt acgtacaaat aatggccttc cttcatttac tcctgtaatg
    28921 ttttcaccgg aaaaataacc ataagaaagt aatcccccgc aatccgtatc gccttctagc
    28981 gctttgttga ataataccgt gaataattta tctctgctta tctccatacc caaagaattg
    29041 gtaaattctt caaaaatact gacccaagca ttgatatccg aagagcagtt attcgtatga
    29101 accatgccta ctagttctcc tgaaggtgta gtgaccatgt cgatttccgg atggacattt
    29161 tctagttctt tttctaatac gatcatagcg aaggcggaag taccagccga aatgttccct
    29221 gtccgtttgg ctacactgtt cgtagcgacc ataccggttc ctgcatcccc ttctggcgga
    29281 caaaccggga ttcctggtct caaatttccc gtagggtcga tcagataagc ccctgattcg
    29341 cttaacttgc cagcttcttc tcctgctact agaactttag gcaaaatgtg atccaagtca
    29401 atcgctacgt gcgctttttc gattaactga ccaaaaatat ttttcatttc ttgatggtag
    29461 gttttcgttg aaggatcgat aggaaacatg cctgatgcat ctcctacgcc tagtactttc
    29521 tcaccagtaa gctgccaatg gacataacct gcaagcgttg tcaaaaaatt aatttctttc
    29581 acatgttctt ctttattcaa aatagattga tacaaatgag cgatactcca tctctgcgga
    29641 atattgtatt gaaatttttc cgttaatatt cgttctgctt cttcagtgat tgtatttcgc
    29701 caagtacgaa atggtactag taactgttcc ttgctatcaa atgctagata accgtgcatc
    29761 attgcactga acccaatcga cccgattgtc gtaagattct cgccatagat ttcgaatact
    29821 tctgctgcca tttttcggta actgacgcgc agtcctttcc aaatatcttc taatgaatag
    29881 gtccagatac cattttctag tttgttttcc cactcataac taccagcggc aataggagta
    29941 aaagatgaat caatcaatac agctttgatc ctagttgatc caaactcaat ccctaaagat
    30001 gtacgaccat ctatgacatc taacatactt tgcgcattta cttccttcat aatgatttaa
    30061 ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta gtagcgctta caatgattat
    30121 tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa gaatacaata aaaaacactt
    30181 gttttgtacg tacataagtt ataaaatgaa attgcttaca aaatctgatc aaccgccttc
    30241 tcgaaattct agaaggagag aatacttatg aaaaaagaaa aaaaagtttc ctcatcgttt
    30301 atttattttt ttggcgcatt tggcggcatt ctatttggat atgatattgg tgttatgacc
    30361 ggcgccttac cttttttaca gcatgattgg ggtttggcag gtaaagcttc tttgatagga
    30421 tggatcacgt cttcggtcat gttaggtgcg atccttggcg gctctttatc tggtcttctt
    30481 tctgataagc ttggacgacg aaaaatgatt cttctttctg cacttatttt tatggctggt
    30541 tctgtccttt ctgcatctgc ccctcacaat ggttcttatt ttctgattgc agcaaggatt
    30601 cttctaggtt tagctgttgg cgctgcatct gccctagttc ctgcttatat gtctgaaatg
    30661 gctcctgcta gactaagggg aagattgtca gggatcaatc aagtgatgat tgcttctggc
    30721 atgctccttt cctatgttgc agattatttg ctaaaagtgt tgcctgaaac gatggcttgg
    30781 cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt tatttttcgg tgttttagct
    30841 ctaccagagt caccgcgttt tcttatgcaa tctggccgct tggaagaggc aaaaagagta
    30901 ttgaactata ttcggacacc aaaagaagct gaacaggaat tcgaacaaat ccagctgaat
    30961 gtcaaacaag aaaagacaac cggaacttct tggcatactt tgtttttaga aaaatatcgt
    31021 tccttagtca ttgccggtat tggtgtagca gtattccagc aatttcaagg agctaatgct
    31081 attttttact atattccgct gatcgtagaa aaagcgactg ggcatgcagc gagcgatgca
    31141 ttgatgtggc cgatcatcca aggaatcatt ttagttgctg gttctttgct ctttttggtt
    31201 attgcagaca agttcaatcg gcgaacttta ttgaaaatag gcggtagcgt gatgggactt
    31261 tcattcatcc taccagctgt tttaggaact gtactggatg ctcatacaaa ttcgttgttg
    31321 attctcttgt ttttatgtat ttatgtcgca ttttattctt gcacatgggc tccactgaca
    31381 tgggtaatca taggcgaaat attcccacta gctgtccgcg ggcgagcatc tggcctagcc
    31441 tcttctttta attggattgg ttctttccta gttggcttgc tttttcctgt gatgactgct
    31501 tctatgtctc aagaaatcgt tttcggcatt tttgggatca tctgcttttt aggggtttta
    31561 tttattcaag agatcgtacc agaaactcgt gggaaatctt tagaggaaat cgaacaaagt
    31621 gcatctaaaa aaacttatcc taaacgtatt tctatccatc attcttaaag aggttgtgac
    31681 agaagtggtc agcttcaaga aataagaagg aaccgcttag tttccagtca caaggaacaa
    31741 tttcaaaaaa cagcttctca tgtttgatga atattgtgac ttgtgaccag ttggtaggag
    31801 aacaccgttt agtggagagg tggtgaaaga aacgttcagc cttgagcgat gacaaaagct
    31861 ctcacaaaca ttgcgacgag taaccgtagg agcaataaga ggcggtaaaa acccgtctct
    31921 tatttttcgt tttctttcac tgtttcccgt tcaactagta caggttcata gagaatattt
    31981 ggctgtgcct gtcctttttc aatcgttgtg acgatccacc gtgcggcatc ttgtcccaac
    32041 ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc ctaaatggct taaagaagaa
    32101 tcgtctgttc ctacgatcga taggtcatct ggaatatgat agccattttc tgtcaactta
    32161 tctagcatta aactagctat ttgatcgtta tagcaaatga tgccatctaa agcttgttct
    32221 tctttcaacc gttgtactgt tttttcagcg attgtttctc gtgtttccgt ggtgaaagta
    32281 atgatatctt ccgtcaaaaa acgcacatta tactgttcac acgcgctgat aaaacctttc
    32341 atcctgtatt ttccttgaag atcgtcgatt tttgtgatga acaacagacg ctgatgtcct
    32401 ttttttatca aatattctgc tgccataaaa ccgactttca catcatctaa acaaatataa
    32461 gaggcattta actcttcata agcagcattg atcatcacca tcggaatgcc ttgttctctc
    32521 aaagcaacat aaagtgctaa gtttgggtta tactgattac ttttcgtagg ctcaacgatc
    32581 aaaccatcta ccccataagc aatcattttc ttcaaacact ctttctccaa ttcgtgatcg
    32641 ttattcgtac tggctaaaag caatgaatac ccagattctc gtaattcatt ttcgattcct
    32701 cggataatcg acggaaaaat ataatcagag agatatgtgg tgatgacacc gatcgttttt
    32761 gttctgttca atggtgtttt ttctttctta tagctgtcat ctacatatgt gcccgaccct
    32821 ttttctttac gcagatagcc ctcatttacc aacacagcga ttgcttgccg aatcgtatgt
    32881 ctgctgacgt catatatttc ttgcagcttg aattctgagg gaatggttgt atttacttcg
    32941 tattcctcac gaaggatctt ttgttttatc tcttctgcaa tatcttgata ttttgccatt
    33001 tgccttgccc ctttttattt gtacgtacat atatcataac gagtctgcaa ggtttattca
    33061 agcaaaaatc aaggtttctt gtgctcataa ggcatacgga acaggttcac caaattctgg
    33121 aaatccttgc tcattccacg taaagcattg cgcgttcgcg tgccgattag gattgtctaa
    33181 cggatcccct ttttggttct tctctggtcg agcatgatag atcaaaatat caaagcaccc
    33241 gtcttctgaa accgtaaacg agttgtgacc aggaccaaat tgcttatttt tttcagaaga
    33301 aacgaatact ggctccgata atttatgcca tgattctttt aataaaagat tcttgtgctc
    33361 atcagcccac agcaggccca tggcatagtt ttcatctgtt gcactaccag aatacgtaat
    33421 gaatattttc ccattgcgag taaggactgc tggaccttca tttactgaaa aaccccgttt
    33481 ttcccattca tactcaggta tagaaaggag gatctgcttc ccctttaacg tccaaggatt
    33541 atccatttcg gatagataaa gattagaatt cccagtaatt tcaggatctt gttgggccca
    33601 cacataatat agctttcctt gagaagagaa agttgttccg tctaaactga aagtttccct
    33661 tttcgttttt atttgtccct tttctatcca atctgtattc attggatcta tatgctgatt
    33721 ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt atagttttat gattgcttgc
    33781 agcaaagtaa atataccatt ttccattgat ataatggact tcaggtgccc agatcaattc
    33841 actcatcggt ccttcttcat gagcctgcca tacaatcagg cttgcagcat gttctaattc
    33901 attcagtgac tttgcccttc ttaattcaat cacttggtag cctggaaccg atcctgtaaa
    33961 ataataataa ccatctgtat gtttatatac ccaaggatct gcccgttgcc gaacgatagg
    34021 attattataa agtgtcttca tcataacaaa tttcctcaca ttttctttat tctaattaca
    34081 ttccaagagt atttttctat ccctacattt aattcgtcat gtttttctat tgaaaaatag
    34141 tttatttcag gttttatttt ttctggttct tcacgtgtat ttttgtccaa cagatcgcta
    34201 ctggttaatg tgtagtgctc tccttgatct agcaaattga aatcatgttc aatcgttatc
    34261 ttttgttgtt catttgtgcg attgatcaag aaaatagtca gctgattgcc gttatctacg
    34321 atcacactgt ccacataagg aacatttttt atcttttcac aatcataaac agggcaaatg
    34381 atttgttgtt tcaatacttg acctttagca tatttcgata agtgttgcat cacataataa
    34441 gttggattcc tccatgcttg tcctttagca tctgtcagta ttaaaggtat tgtgtttact
    34501 aacaatgatt gacaactaat ctttacgcga tctgcgtgtc gtaaaatagc cagccctaat
    34561 gaaccaaata ataatgtatc ttccatagta aagtaacacc aatcgatggg actaccgact
    34621 tcaaaatccc tatgtctttt ttccggttgg gcatgtacat tccattcatc aaacgctaga
    34681 tacattgttt tctcacttct caaatatcct tttacataat cacaagtagc aacgatttct
    34741 tcaatttgac gatcgaatcg ataagctttt gcaaggtatg tcgctgtatc atcacgctca
    34801 tttttaggag gtttagtcaa gtcttcttca tcatacttat caatatagtt atgcaaagct
    34861 aaataatcta cattctcata agcttcttct aatgcgatac gatcccattc agggtacgat
    34921 ggtaaacgtg gcgtagagct tccaactaag attgtttcaa ttgaatcatc cactaacttc
    34981 atgacttttg aggcttcatt tgctaaccta ccatattcat aagcactctt agcccccact
    35041 tgccatggac cgtctagctc attccctaaa caccataatt taacgccaaa aggctttttt
    35101 tgcccatttt ttattcttaa atcactccag aaggttcctt ttggaaaatt acaatattct
    35161 actaaatttt ttgcttcatc aattcctttt gttcctaaat tcaccgtgaa tatcggtttc
    35221 gcttgtacat gactgatcca tttaaaaaat tcgtggagtc caaaagaatt tggttctata
    35281 cctcgccatg ctaaatcaat tttttttggt cgttgttctt tcctacctac agtatccacc
    35341 caatcataac cagatgtaaa gtttcctcca ggatagcgaa tgacacctaa ttctaattcc
    35401 tttacataag agaggacatc ttgtctaaac ccgtcaccat cagctgtttc atggcccggt
    35461 tcataaatcc ctgtatagac gacacttccc atatgctcaa caaatgagcc aaacagccga
    35521 gaactgatct ctcctaccgt aaaatcctct ctgattttta ctttcacttc ttcgtccaac
    35581 agctttccct acctttcatt tcctatagtc aagataatag atacaagttt ccatacgcca
    35641 ccagcaaaga taaaaataaa aggagggaaa agaaatatga gcagtacaaa tcctaataac
    35701 aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata ctttccatgc actttgttca
    35761 aaaatttgta aaaatgtgat atctttgttt tcagaaatca catatatcca ggataaaaga
    35821 aacatggaat aaataagtaa aactacatat ccactaattg aaagtacgat tgtctgttct
    35881 tctaataatt gattaacaag aaaataaata atgatcgtta acagactaag gaacaagttc
    35941 ttaccaaaat gttgctttat tccttgaaag aaaaaataaa tgatatttcc tgatccctca
    36001 cctttccatt gcgccattac ataaaaaaca gcacatgtag caggcacgat agtaataaca
    36061 ggcaaacaca cgattaccca gagaatacct aataagatgt aatcagaaac cacaatcaac
    36121 tgtccataaa atttagtctc aactattttc atcttcctca ctctttcatt ccagacatag
    36181 ctgttccttg aacaaaatat ttctgtgcaa aaatataaac cactagtaat ggcagcattg
    36241 aaataaccgt tccagccatt acaggtccat aaaaagaaat gttctgccct gtaaataaag
    36301 ctaaccctgc tggtaaagtt ctcatttttg aactacttgt caatatcaat ggataaagca
    36361 agtcattcca actattcatc agcgtgaaaa tccctaatga taaaagagta ggtttgctca
    36421 atggcaacat gattttcaaa aaaatattaa actcacttaa tccatcaatg cgtgctgcct
    36481 cttccaaaga atctggtaaa gtaataaaaa aggaacgcat catgaagata ccaaatgcag
    36541 ttgtcatacg aggaatgatc aaacctgcat aagtatcaag catgccaagc aaattgactt
    36601 caataaatag aggaatcatg aagacttgga atgggatcat cattgtcagc aaaacgaaat
    36661 agaacaaaac tgattttcct ttgaaacgca tacgagcaaa tgcatacccc gcaagagaat
    36721 caaagaatac agaagtgatc actgtccctc cagcaaaaat cactgtattt ttgatgtaat
    36781 ctaataaagg gatcgttgtc caaaccttaa tatagttatc tagggtatag ctatctgcaa
    36841 atattctagg cggaaatgag ataatgtctt tttcgtgttt aaaagaagaa ataatcagcc
    36901 acaaaaaagg aaacgcaata accaacaata taactagcat actaatcgtt cgaatgattt
    36961 ttttaccact aactgctttt cgactattca cagtaactgt aatcgagctt tctggtgcca
    37021 tacaggtttc cctcctattt catttgtttt tctttccgtg acatgtacac attagtggag
    37081 atagctatag ttgcaacaat gataaacaaa accgaagaca gtgctgatgc atagcccaat
    37141 tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa cagctgtttc tgtcttaaat
    37201 tgtgggccac cagcggttgc aacatatact tggtcaaaca cttgcatcga accaatcaga
    37261 ttcgttaaaa tacaaaaacc taaggaggga acgatctgag gtagcgtaat gttaaaaaac
    37321 tgctgtcgat ttgttgcacc atccatttga gcagattcat acaaagaggg agagattccc
    37381 tgaatagcag tcaagagaat cgtcatagta acaccaaaat tcttccaaac agtcatgatg
    37441 gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt gagggatcgg caagccgact
    37501 tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa gcaacataga aaaaataatc
    37561 gcaattgacg tcaatgaaca aataactgga atgtaaaatg tcgaacgaca aaaacgattg
    37621 aatctggtat ttttagataa tgctactgct gctaataaac caacgataat ttgtgttggc
    37681 gtttcgagta cggtgaaata taaagaatga atcaacgaat taactgctcg ttcatcacta
    37741 aatatacgtg tgaaattttc aaaacttaca aaatttcttg atgtaaagaa aatattcaaa
    37801 tctgtaaaac taatagcaaa tgttcctaaa agcggtatga cagtaaataa taataaaact
    37861 aaaaaagatg gagttaaaaa caaatacgca gcacgatcag gacgattcca atatttggtg
    37921 aatccgttca tatagtccac ctcatctttc attttgataa aatatacaat cttggtttaa
    37981 acgaatctga ataagatatc cgaacaatcg ctgtgatttt tgcaataaca cctatctgca
    38041 aatactaggt cgatcattca gatatctgta tcttgccaac agtctatcct gtataaagtt
    38101 ctgtactatt tttgcagtaa ttgattgatg gcatcatctg catcgttcat gagtgtctca
    38161 ggatcttctc ctgctaataa tttttcaatc agtggattga tgatatcgtt attgatcgca
    38221 gagatttggg taaaacctgg taaatatggc tctgcatagt ccatttgttt cgataattca
    38281 gatacaatag gatcattttt tacttcttca tcttttgcta ctgaatccaa gtaggcagga
    38341 aaaccatttt cgatactcca ttttttacca atttcagttg tattccaata tttcaaaaac
    38401 tcatagacag cttctttttt attattatct gtacttgccg gaatggcata tcctacgcca
    38461 tctaaaatag ctgttttttt gtctgaatca ctcccttgtg gtaaggtagt cacattataa
    38521 tcaatctcgt ttgctttcaa tccgttattt aaccatggcc cattgatttc aatggctagt
    38581 tgaccagcat tcattaaatt gtccatttcg gcgccagaaa tcgattctgg accagtcttg
    38641 tctacatgga tcattttttg gatcgctttt aacgttttaa ggttctcagg agacgcaaat
    38701 tcagacttag tataatcatc gttcactaat ttaccgccat ttgctaacat ccagttatat
    38761 aacggtgcag ttccatcctt attgaagaca aaacctgata catttttact actatcagtt
    38821 aattttggtg ccatttctgt caattcttcc caagtagtag gtggttgttc agtatctaat
    38881 ccagcttgtt taaacaaatc attattccaa tacatataca tgccttgtac ctgcatgggg
    38941 acaaaatact gtttaccttc tacttcacct aattcaatag ccgtttcttt aaaatcagat
    39001 ttatctactc catcatattc ccagaaatcg tctaatggtt gtaccgctcc gttttctaca
    39061 tactgtgcaa aatctggata atttaatgca ataaagtcag gtgctttttt agaagaaatc
    39121 gctgtaggaa gtttttcatt caaattggcc caagtcatga tatccatttc aattttgatg
    39181 tttttatcat ttgttttatt aaattcatta acgatttctt gcaatatttc accatcagat
    39241 gcagtaaacc cattccaaaa agtcaaagtc acttctttat cagaatcaga ttctgctgaa
    39301 cttccgccac cgcaagctcc taaaatacct gttgcagcta ccaacaaaga gccaattaac
    39361 cattttttac ctttcatttt ttgttcctcc ttcatctgat aaacgacatc tgaccacatt
    39421 ccaagatagc ggtttcaata aacaattgat tttcccatct tcagctttta catttgttga
    39481 atgacttggt tttacttgtt cacttttagg aagattagtt gcttttgtat caaatccact
    39541 catttcagaa aaatcaatca ctccctctaa tgataattca gacaagtgaa cagttagatt
    39601 catttcttct tctccacggt taactgcaaa tatgacaagt tctttgtcat ttattacggc
    39661 tatactttct aaatacggca cttcttcaaa atcttccgtc gcatagtttg ctgaacgaat
    39721 ttgtgggctt aacacctctc cttgtccata cacagcagct tgcataaatg gataatagat
    39781 cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt ggtgcaataa cgtttaccaa
    39841 ttgagcaaga caagcaatct ttactctatc tgcatgtttc aataaagtga ttaatagaca
    39901 gcctaatagt aatgcatctt caaaattata gttatcttct agtaaaggag gagcaatctg
    39961 ccaaggcgct gcttgctcat cttgttccgc tgaatgatac caaatattcc attcatcaaa
    40021 ggacaggttg atctgcttct tactatgttt cttcgccttg actgtatcgc acatcgctgt
    40081 cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca agataatgag aaagatcatt
    40141 tttttggttg ccataatatt gatgcagaga caaataatct atttgatcgt acgcttgatc
    40201 aagaactgtt aactcccaat caccaaatgt aggcatttgc cgattcgaac ttccgcataa
    40261 aaccacctca attgaatcat ctacgagttt catcgcctta gccgtttcct ctgctaaccg
    40321 tccatattct tccgctgttt tatgtccaat ctgccaagga ccgtccattt cattccctaa
    40381 acaccatgtc ttaattccaa aagggctctc ttgaccattc gaacgcctta aatcactcca
    40441 ataagttcct ttaggaaaat tacaatattc gactaaatta cgagctgcat ctactccacg
    40501 agtacccaga ttgacagcca tattgatttc ggcccccaca tgctgacacc agttataaaa
    40561 ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa gctaaatcca accttctagg
    40621 tctatttgct atgggcccaa tactgtcttc ccaacgaaat cctgacacaa aatttccacc
    40681 aggatatcta atcatgggaa tgttcaattc ttttaccaag tgaatgacgt cttggcgaaa
    40741 tccactttta ttagctgatg gatgatctgg ctgataaatc ccttcatata ctgctcgtcc
    40801 catatgttca ataaatgaac tgtacaaccg cggatcaact ttgcctacac gcttgacact
    40861 actaatcaac attgtggctt ccataactga cctcctttaa tttatacatg aataatacac
    40921 ctcgttgaaa gcgttgtcaa taatataaat gttaaactta cttatttttt atcgatttta
    40981 attaaccaaa atattaataa tgattaaaac aaataattta atataacttt tattaacaaa
    41041 tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta caaataaacg
    41101 aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag atcatccagc
    41161 tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga gtgagcagtg
    41221 ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa acagaaaaag
    41281 taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatattagt tttccagaaa
    41341 aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac tatacaaatt
    41401 atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg aaagtggatg
    41461 aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg tttaccagcg
    41521 gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg gagatgttgg
    41581 aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg ccttcagata
    41641 tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga gattttgctg
    41701 atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa tacggcttgc
    41761 tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc ttgtcaaaca
    41821 tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc gaagtgaaag
    41881 acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt aattacgatc
    41941 aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt tataaaacaa
    42001 aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt ccagaaatca
    42061 tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca tgcttcgatt
    42121 ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat ttcattatat
    42181 gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag atctgcagtc
    42241 ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg tttcccttgt
    42301 cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa tttcgtgaag
    42361 aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc tctttctcca
    42421 gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt gttaaaatcg
    42481 ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc tggtttgata
    42541 tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc atcttcttca
    42601 tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc atcttttgtt
    42661 tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt agaatcttgg
    42721 ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa atatgctttc
    42781 ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt gacacctaat
    42841 tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct gtatgaagtt
    42901 ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag cattcagtag
    42961 acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc catagtgaat
    43021 ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg taaactcttt
    43081 ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga acagttttat
    43141 tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga gggtcttcat
    43201 cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg atgcgccaca
    43261 cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg atcgtatcca
    43321 tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca agggctggct
    43381 gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta gctttttgca
    43441 tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt tttgcttccg
    43501 taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc atatctccac
    43561 tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc acgcgaatcg
    43621 cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg cagtctttat
    43681 gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg actaacgaat
    43741 gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca ttgcttgttg
    43801 catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc agttcttgtg
    43861 cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg atacgagctg
    43921 cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt tctactaatt
    43981 ggcagtgcgc taaaggccct tccacttgga tcaatggttc atttgcaaaa actagttctc
    44041 cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac tccaaaaaac
    44101 cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg aacgtcagat
    44161 tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg tggttaaaag
    44221 gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct aattcccagt
    44281 atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg tcgtctgggt
    44341 aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac ccattataac
    44401 aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca tgttttacgg
    44461 atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat tggtatttaa
    44521 cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag aaattagcgt
    44581 tttctatttt tcttacttga taaaataagt agattttcct actacaatcg tagccgttaa
    44641 gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa tggcaaaagt
    44701 ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa acggatataa
    44761 cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac cttgcgttga
    44821 aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag aaagcagctg
    44881 tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt tttttattgt
    44941 atacattaga tatggagcag gttaccataa agatgattat tagtcgttat taaatagaat
    45001 ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg aattgtattc
    45061 cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct cgtgtcattt
    45121 ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc ttcgtattga
    45181 tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt cgatctaacg
    45241 aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact actttaggat
    45301 aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc atctcattcc
    45361 tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct ttcctagcat
    45421 taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt atcacttaat
    45481 agtataacat aatttcatga gaaaaaaata caattttttc ttgaccatct cgttacgtaa
    45541 aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa agaaaatgtc
    45601 agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg gactgctaaa
    45661 accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag tcgatcgtct
    45721 gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc aagaggtctt
    45781 agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa tgttgctgga
    45841 aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg atatgtacga
    45901 aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac aaaagttgaa
    45961 agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa aaaaggttac
    46021 agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa tgcaaactgt
    46081 tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag atgaaagctt
    46141 agcagaccgt atcttccaag ctcataagaa gtggttatct tacagctggc ctgaatatca
    46201 gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt tcactgcata
    46261 ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta tccaaaaaaa
    46321 ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta ataacaaaac
    46381 agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat tctttcacaa
    46441 gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg tattttcact
    46501 ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc ttccagatgg
    46561 tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg tccaatcacc
    46621 actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat atccgaattt
    46681 gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt tcctttctct
    46741 gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag ttgatgacat
    46801 agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa cgtgatgccc
    46861 aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga ttgttctcga
    46921 tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac tagaggtcca
    46981 tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat gatcaatcca
    47041 tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt ctgatagccc
    47101 gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat cccactgatc
    47161 aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct tttcgttttt
    47221 ccataactta actgtctggc atttgcatta gggatataat cgacttcttt catgaccgca
    47281 agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac acgtgaaata
    47341 gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac tgctgcttcg
    47401 ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg cattcaaaaa
    47461 actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa tatatatctt
    47521 ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta ttcaaccctt
    47581 gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa aaataattgt
    47641 tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct tttgatactt
    47701 tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa agaaaagttt
    47761 tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt ataatgatac
    47821 tgtaaatata tctttattat tagtgaatgg atatttactt ttaatagata aaatattata
    47881 actattaaaa ataactattt tcattataat tacacacaaa aaagagcgtg agacaaaagt
    47941 aaagaacact tttgtctcac gctctaaaca cgtataaaca gcggaagcag aagcaactcc
    48001 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa tttccgtgaa tttcttctta
    48061 tttcttggag ttaaacgctt ctgttccagc ctctttttat ctgtctttca ctaatgatca
    48121 gttgtagata agcaagttgc tttttcttac atcataccgc ccatcattga tggatccatt
    48181 gcaggagctg ctggcgctgc tggttctggt ttatctgcaa cgactgcttc tgtcgttaat
    48241 aacaaggcag aaacggaagc ggcattttgc aaggcagaac gtgttacttt agttgggtct
    48301 acgatacctg cttcaaccat gtttacccat tctccagtag cagcattgaa accagtgcct
    48361 agttccacgt tcttcaattt atcaacaatc actgatcctt cataaccagc gttttcagcg
    48421 atttgacgaa ttggttcttc taaagcacga acaacgatct tgatacctgt tgccacgtct
    48481 ccttctgctt ctacagcaga aactttgctg ataacgttca ctagagctgt accaccacct
    48541 gagaccattc cttcttctac tgcggcacgt gtagcattca atgcatcttc aatgcgtaat
    48601 tttaattctt tcaattctgt ttcagtagct gcaccaactt taacaacagc tacaccacct
    48661 gctaatttag ctaagcgttc ttgtaatttt tcgcggtcaa aatcagaagt tgtttcagca
    48721 atttggtttt tgatcaattg cacacgagct tcgattgctt ctttttctcc agaaccttct
    48781 acgatcgttg tattgtcttt atctacaaca actttagaag catttcctaa gttttcgatt
    48841 gttgcatctt tcaattctaa acctagatcg tcagtaatta ctgtaccacc tgtcaaaata
    48901 gcgatatctt caagcattgc tttacgacga tcgccgaaac caggagcttt tacagctact
    48961 acgttgaatg ttccacggat tttgttcaat acaagagttg gcaatgcttc accatctaca
    49021 tcgtcagcaa taatcaacaa tggacgtgat tgttgcaaga tttgttctaa caatggcaga
    49081 atatcttgga tgttagagat ttttttgtct gtgatcaaga tatatgggtt ttctagaaca
    49141 gcttccattt tgtcgttatc tgttaccata tattgtgata agtaaccacg atcaaactgc
    49201 ataccttcta caacatctag ttctgtttcg atcccttttg attcttcgat tgtgatgaca
    49261 ccgtcgtttc caactttttc cattgcatca gcaatcaaat gtccaacttt atcagaaccc
    49321 gaagaaacag ctgctacttg tgcaatggct tcttttgaat caacaacagt tgagatatta
    49381 tgcaattctt cgactgctgc ttttgttgcc aattcgatcc cgcgacgaat gcctagagga
    49441 ttagcacctg cagttacgtt ttttaaacct tcgcgaacga ttgcttgtgt cagaacagtt
    49501 gctgtcgttg taccgtctcc tgcaatatca tttgtttttg atgctacttc tgatacaagt
    49561 tttgcaccca tgttttcaaa atgatcttct aattcgattt cttttgcgat cgtcacacca
    49621 tcatttgtga tcaatggaga gccgtaagat ttttctagta cgacatttcg gcctttaggt
    49681 cctaatgtta ctttcactgt atctgctaat ttatctaccc cgcgtaacat agctgcgcgt
    49741 gcgtcttctg caaatttcaa ttcttttgcc atgattgatt cacctcattg attatttttg
    49801 attatttttt tatcttccaa atttactcaa caatcgccat gatatctttt cctgcgacga
    49861 tcagatattc ttttccttcg tattttactt ctgtaccggc gtatttttcg aacataactt
    49921 ggtcgcctgc ttttacagcg gcaggaactt tttcaccgtt ttctaacaaa cggccttcac
    49981 cgactgcaac aactgtccca gtttggggtt tctctttagc agcagaagct aaaacgattc
    50041 ctccgacagt tttttcttct tcttttgcga cttcgataat gacgcgatca cctaatggtt
    50101 ttaacacgat aaatccctcc gataactctt tttttaatct gttagcactc ttaaccttcg
    50161 agtgctaacc tacattttta ataatactca ttctccctct tttttgcaag cttttaaatc
    50221 aaagaaaaga aattttattc tgacagctgc ttattgaaat tatgatataa ttgaaatcaa
    50281 tcgatataag cttgccttgc atgcttgtta tcccttaaaa ggaggaatta catgtcttta
    50341 aaaaaatata gcttcctcag cattttttgc tatggtctaa tttttatctc acccttgatc
    50401 ctagcttcga taggacttgt aaaatctacg tctgaattga tcacagcaac tgcagtcatg
    50461 tatatactag gcgccgttgt cctagctatt ttttatttta agcaacgtga acccttagcg
    50521 atcgaatcag cggtaaaaaa agcttctgct ccaaagattg ttatttatgg attggttgga
    50581 atctttgtcg cattgattct gcaaagcatc gctgtcatgt tagaatctgt attgtttggc
    50641 aaagcaaccc cttccgagaa tacacaaaat atcatccaga tgatcatgga agcacccgct
    50701 tttattttgg cgacaactgt tgctggaccc atcatggaag agttcgtatt ccgccgcagt
    50761 atactgggga tcatcagtcg atattcaaat ttttgggtcg gagcagtgat cagctctttg
    50821 ctgtttgcgt ttgctcataa tgatggtcat ctgctaatct actttttcct aggtttcttc
    50881 ttcagcctag agtacaaagc aactggtcgg atctggacat cgatgatcac acacgtagga
    50941 atgaacacat tagttgttct tgttcaatta gctgtccaaa aagggctagt ataaacatga
    51001 ctataaatac tcctgtcatc ctagattggt gattttccca atctaaagcg acaggagtat
    51061 ttttgttttt tagtcgttca tcttttctgc tgctccatca ttttttgtct gtctttcctt
    51121 tttgcagttt gcagaatcta tttttttcaa aaatgggaaa taaatgaaaa aactaattga
    51181 caacacgata ccttgtaaaa tcgctgcagg cactccgcct actaagaaac ccgagaggat
    51241 cggcggtgtt gtccaaggga cttggactgc tgtaaataaa ggaacgaatc caaaatataa
    51301 agcggaatag gtaatcaatc ctgaaacgac cggtgcgaaa ataaaaggaa cagccatcat
    51361 cgggttcatc acgatcggtg tggaaaaaat aattgattca ttgatgttga aaaaggctgg
    51421 taaaagagca agatttccta attctctgta tttcttcgat ttcgcaaaaa ccgtcataaa
    51481 aaatacagcc cctaacgtca atccagcacc cgtaaccgtc atgaactgat cgagaaattg
    51541 ctgagtaacg atatgtccgc catttgccac cgtcaactct tgtcctgagg ctaagatttc
    51601 tgcatttgct aatgaattcg cttgcaagac gggactaacc aacccattga ccatcgcaga
    51661 tccatgaaga ccaaataacc ataaaaacgg aacaaaaaaa cctaataaaa gcacaccaaa
    51721 caacgtgtct atgaacgatt gaatgggaag ctggacatat gtccatactc cttgaacaac
    51781 agtcattctg aacaaatgtt gaaaaaccgc tgaaccgata agcgtaaaag tgactataac
    51841 aatgattgga atcaatgaag taaaagactg ccaaacattt tcaggtatct tctctctttg
    51901 cttccaccct ttcgcaagtc ttactaaacg tgcatagccc catccgatga tgagcccagt
    51961 tgtaactgct cctagtatcc cttcagcacc tgtccagaca gtatctatct gagtatttag
    52021 tggctgaata agcagtagat aagcagacaa ggctgttatg ccgactggta agcgatcgat
    52081 gtcttcttct tttgcataag cgtatgcaat ggcaaaaacg atccaaaagg ctatcacatc
    52141 aaatgtagcc tggtatactt gtgtaaaata catagaaaat cctgttttgt ctatccactg
    52201 agcgatgtaa tccacaggaa aatgagcaaa taagaaaaac agcgagccaa caataattat
    52261 tgggatcgtg taagtcattc cattacgcaa agcagttaag ggtctaagat tcacaacaga
    52321 tgttatcatt ggtaaaaagg cacgatctaa ccactctttc atttcttatc tccttcaact
    52381 tctattttta tcaatcagtt ttcttaattt aactataccc aatgatcacg aaccatgaaa
    52441 ggaatgcgca ctgagctttt caaaaatcgg attctttgac gaatattcag caactgctta
    52501 caatggacag gaataaaaaa ggaggttttt tatgaaaatt gcactcattg gagctactgg
    52561 ccatgcaggt tcatttattt tagacgaagc attgtccaga gatttagacg tgacggcaat
    52621 cgtgcgtcat ccagaaaaat tgccatcaga tgttccgttt atacaaaagg atttatttga
    52681 gttgacaact actgatcttg catcttttga tgttgtcgtt gatgctttca acgctcccaa
    52741 aggaaaagaa gaaatgcatc aaacttcttt gtcccatttg attgagatgc ttgaaggaac
    52801 cgatacacgc ttaattgtcg ttgggggtgc ctcttcgtta ttcttagata cagaaaagac
    52861 gactcgtatg attgaccaag tacctaaaaa tgcacccttc tatccaacag ctttcaatat
    52921 gtacgaagga ttgttaaaat tgaaacaatc taaaaatcta cattggactt atatcagtcc
    52981 agcttcttta tttgatccac atggcaaacg tacaggtact tatttgttaa gtgatgatta
    53041 tttacaaaga aacgctgcta gcgacagtac gatcagcatg gcagattacg cgattgcatt
    53101 agtagatgag atactggatg agaaacatga aaagcagcac atcagtgtag tcagtcgata
    53161 atacggttat ttttttaata tgctaaaaaa ataaaaatag aagataaaac caaccctgat
    53221 tgattttatc ttctgctttt tatattttta ttgattgaac tgtttaaatc gaaaatagct
    53281 cagttgcttc atctggatct gtatcataga tttcaaaatc atgtccaacg ccaaaatctt
    53341 tttctttcaa aacattttcc attgttaaat gagcaagttc tttcatattg ttttccttgc
    53401 taagatggcc aagatagatt cgtttggtcc gattaccgat cacatcagtc atgacaagtg
    53461 caccgtcttc gttggaaaga tgtcctctat ctcctaaaat acgctgtttt aaattccacg
    53521 gataaggtcc catgcgtaac atctcaagat catgattact ttccatcaaa taaccatcgg
    53581 cattttcaat gacacctcgc atataatcac tgcaatatcc ggtgtccgtc aacatgacga
    53641 atgaccgatt ttctttatgg aaacgataaa attgcggagc cgctgcgtca tgggagacgc
    53701 cgaaactctc aatatcgata tcgccgaatg ttctgacttt ccccatctca aatagatgtt
    53761 tttgttccac tggtacattt ccaatcagtg gttccatcgc ttcccaagtc tgctgattgg
    53821 caaaaacatc caaatgatac ttacgagcca agacacccac gccatgtata tgatctcgat
    53881 gttcatgcgt cacaagtatt ccatctaatt ggtccggtgt ccgatcaatt tgagccatca
    53941 aggaagtgat ttttttaccg ctaagtcctg catcgaccaa aagccgtttt ttttcacttt
    54001 cgatataaag tgaattaccc gaacttccac ttgctaggat actgatttta aaagctggtt
    54061 tttcttcagc catttgactc ctacttccct tcttactttg taaacttttt tcattatact
    54121 cgctttttta ttgatcttcc acttttggta cagtattgtt tgtcacgatc gtattgctga
    54181 aagcgttgac gctctcaatt tggaccactt tatcgcttgt ttcgatcgct acatgccaaa
    54241 caggaacata cacatttttt tcccggactt tcagaataca ggaataagct agctgacgcc
    54301 ataaaatttt tgatcctctt ggtattttgt tattgatgta caatgtattg attgcttcct
    54361 cttccgtata cagttccgtc ttttcgcgca gctgctcgat atcagataaa tgcgtctgca
    54421 tgtatttcgt gatgctataa tcattatcgt ttttttctaa agaaagaacg atttttgctg
    54481 tatcatcatt gaatgggatg ccttcatact cttggcttgc ttggatttct agtggttctt
    54541 cttctaaatg agaaaagttt ttcgtgtaca cataatcatt accgaataaa atattgtctt
    54601 tgttagataa aaaatcttcc aaggaactac taattttatc tggatcgatc acgttctttt
    54661 cagcatctga actggataaa gatttcgtca aaacatcacc gtctaaaaac acaccacttg
    54721 ccagcaagcc gcttttacca gccttgcgat aattcgctaa tgaagtgcta agattggtct
    54781 gctccgcgct aagataatag ccttgcttgt tctcagacga aatcttcccc gtgtaagtga
    54841 tatcgtcgct agccagacgt tgttggattg attcagtctg attagagcga atgacattgt
    54901 tttcttggta taccgcgtca tgataactgc tgaataaaaa cacattcaac cctaaaaaag
    54961 cgacgaaaaa tatccattcg atttttttga aatccaatta attcgcctcc aatccaggta
    55021 atttttccag aaggtcgtta gctgaatacc agttatcttt atatttcaca taccattcag
    55081 gtaagagatc aacgacacga ttcgtttctt tgacattttg ccatgtatag ccaacgatca
    55141 ctgaaccaat cttgttgcta tctgctccct tagcttttaa ctgctggatg atttcattcg
    55201 tacttgcaag ttctacttct tcgtctgaag gtatcggcac ttgaatcgta ttcaaactgg
    55261 tttggatatt cacttgcaca gaaccattcg tttcaccgcc aacttccaca ccaatctgtc
    55321 ctctgctgtc tgtcccaaaa accgggaagc cttccacgaa tatccgataa tcgatcgtat
    55381 tgtcactgcg gtcaaaatat ctgaggttcc ctaaagaact gcctaatttg ctaatatagg
    55441 ggaaactttc agaaaataca ctagcatttt cttttttctc tggcagctct ccacgaaaat
    55501 caactacttg cgtttccgaa tggatagaca ttgtttcatt cccaccgtaa aaaatcaatt
    55561 cattgctttc atcattattt ttcacgctat ctggtttctg gaaaaaagca tttctaaaaa
    55621 gtgtataggg ttgctgtgac aggatataac tatacttctt cagtttgatc gaatgtttcg
    55681 tagcatattg cgtgtcgatc gtcgatggtt ctgttgtcat ttctgtccat tttgtgtctt
    55741 ttgctgttaa ttctttattg atatctgtcc aattcaacga aatatttgct tcatatacga
    55801 gatggttcga ataattcaaa aatcggattt ttttatttcc ttgatcgaat tgtattctag
    55861 taaaatacat attttcgctt tcttttgcca agttcgacac atctaaatct aaatcaaatg
    55921 cttccttgta ttctcttagc aaaaaaggcg cattataggc caactcaaaa ccagagttca
    55981 gatttttcac ctgttcaaac tgtgatttgt ctcctgaaac tacttcagaa agctttccaa
    56041 atcttgcttt atcgatgatc aattgcaatc ggtacacgag attttcactg ttggtttctt
    56101 ggatcattcc cgaatggtgc catgtaacat gaagcggaag aaagacttcg gaagtttttc
    56161 gattgctttg agactcgatc atttgagtgt ttgtctcatc tacgttgatt gaactttttc
    56221 ctgctgggct cagccagatt gcataagaga agtatatgct taacgcaacc atcaaaatga
    56281 gtcctatacg aacgactttt tcagagattc tcattcccac cattcctcct cataaggttc
    56341 atatggcagc gagatataaa aagtcgaccc ttttccttcc ttgctctctg cccaaatagc
    56401 gcctcgatgc gccttaatca cttctctaga aatagctaat ccaagtcctg tcccaccttg
    56461 tgctcttgca cgtgctttat ctactcgata gaaccgctcg aacactcggt tcagatcttt
    56521 tttaggaatc cccaatcctt gatcggtgat acttaaaatc acgttattat gcgtttctaa
    56581 taagcgacaa gtaatcgttc ccccatctgg tgaatacttg atcgcgttat tcatgatatt
    56641 atcgactact tggatgattt tatctgtatc tacttcgacc caaagatctc tttgcgtaaa
    56701 ttcacgacgg atcttataat ttttttcctg atttcccacc atcatatcaa accggtcaag
    56761 aacaaaattc actaactcgt tgaaattgac atattctaat tgaagctggg tattacctgt
    56821 atccatgcga gatagattta agaggtcatt gatcattcga atcattcggt ctgtttcatc
    56881 tagcgttacc ttcaaaaaat ttggtgctac ttcctcgtct ttccacgcac cttcgctcaa
    56941 tgcttcgata taactacgca tacttgtcaa cggtgtacga agttcatggg aaacattaga
    57001 gacgaattca cgacgttccc gttctgtttt ttcttgttct gtcacatcat gcaagacagc
    57061 tactaatccg ctgataaaac ctgattcgcg acggatcata gaaaagtcta ctcgcaagat
    57121 catctgatca ctttccaatg tactggttga acggtcaagt accatttcgt ttggatcttc
    57181 taataatttc cgcaacgtat attcttcttc gatatctaaa agtgtcaaaa tcgattgtcc
    57241 gattgcttcc tcgtttttga tatctagcaa ggaaacagcc atatcattga tcgtaatgac
    57301 tttacccctg cgatctgtcg caatgacgcc gtctgtcata tgggatagca cgctatccaa
    57361 acgattgcgt tcagcttcca ttgtatcttg agcttcttca atacgttcag ctaactgatt
    57421 gaaggtatca gccagctgtc caagttcatc ttgtccatgc actttgactt tccgactgta
    57481 gtctcctcta gcgattcgaa tggcttgttc tctcatttca ccgatcggct gtgtaatcga
    57541 acgagcaact aaaacagata cgatcattga aataaatgcc gcaattagtg aggcagtaaa
    57601 gaaaatgact gctgtgttat tgatttccga atatttttgt tcgatattac ttttgacgta
    57661 aagagccccg ataacggcat cgccggtcgg ggacaggatc ggttgaacat tgatattcac
    57721 ccgtttatcg tcgttatcta aagcaacata acgtttagtc gtgaaatcat tgatatcaac
    57781 ataatcattt ttctttccca cagcgctttg ttcattgaca tcggttgttc ctcggatgat
    57841 ccctttttcg tcaacgaccc gtatttcgat gatatcgcta gtcgcactgt tttcaataaa
    57901 tcttttgata ttcgcattta cctcttcatc atctgcatta tcttttcgat tcaattccgg
    57961 gctgattgtt gttgctaatg attccaccgt ttgattcata tctttaataa atgtattgat
    58021 cgtggaacgt tctaaaccac gtataaagta ggcaccaata atttcgatcg agatcaataa
    58081 aatcagaata aaggtaatag cgatcttaaa attgactgat cgaaaaaagc ggacttttct
    58141 tttcatccaa ttactcctgt tctgggttac gcaaatagta accgacacca cgacgtgtca
    58201 ctaaataatt tggatggctt ggattatctt cgatcttttc acgcaaacga cgtacagtta
    58261 catccactgt tcgtacatct ccaaaataat cataacccca aaccgtttgt aataaatgtt
    58321 ctcttgtcat cacttgtcca aggtgcttcg ccaagtaaaa gagtagttcg aattcacgat
    58381 gtgttaactc gattgtttct cctcgtttcg tcaccatgta agcttctgga tggatcgtca
    58441 aatcaccaat cgtcaattcc gcaggtgctg cctcttctgg ttccttcgca gccgtggcac
    58501 cgcgacgaag atttgctttg acacgtgcaa ctaattctcg gttggaaaat ggcttggtca
    58561 cgtaatcatc ggcgcctaac tctaagccta ggactttatc aatttcagaa tctttggctg
    58621 ttaccataat gatcggcata tcataagttt tgcgtacctc acgagcaact tctaagccgt
    58681 ccattttagg tagcatcaaa tcaagtaaaa tcaaatcagg ttctacctca gcaacttttt
    58741 ccaaggcttc ttccccatca tatgcagtat aaacgtcata gccttctttg gctaaattga
    58801 atttgacgat atccgaaatc ggcttctcat catcgacaac taaaactttt ttcataaaga
    58861 gccacctctt atatattatt gtcatacctt ctcattatac cgtatttctg cttttgtttc
    58921 atctttgcct ataaaacaaa agagaaaatt aaaatgaaag tgcaaaatac tttattattt
    58981 tttgttctaa cttcaataaa aaaggaaaac actgattttc acctcagtgt ttcctttttt
    59041 tgatttatcc ttctaaaact tgtacaccga cacccaataa tcccggacca gtgtggacta
    59101 ctaatgccgg agaaatctgg ccaaagtaga tttggtttgc ttgtgggaac tctcttttaa
    59161 gtctcgcttc catttcaatt gcttcttctt tcgcatcccc atgggcaaca gctaagttga
    59221 agacctttgc attaccgatt ttttctttga cgtaagcaac cgttttatcc aaactttttt
    59281 tacgtcccct agcttttcct actgtgtaat aaatcccttc ttcattacat gagatgattg
    59341 ggttaagctt taaagcggtt cctacaatag aagcaactaa accgatccgt ccaccttttt
    59401 gaagatattc caatgtcgca acattaaaga aaacttttgt tctttcgact aactcattca
    59461 gattactgat cagttgatcc cattctactc cttcttccag ccattttgct gcttggatcg
    59521 cagaaaaacc tgcaccaata ccaatatttt tcgtatctag tacatatgct tccaattctt
    59581 cttgctgttg tgcaattaaa cgaaccacat tatatgtccc gctcaatcca ctggaaattg
    59641 ttaccgcaag taccttttca taaccgtctt ttttgatttg ctcaaagatt tctgtgatcg
    59701 actcaccatc cggcaaagat gtaccaggga tttcctctgg caaacgttga tagatctctt
    59761 caggggtaat gtcaatttta tctgtatata cctgatcttt ataaataatt ttcaatggga
    59821 tcatatacat ctcgtatttt tttatcaatt cttctggaac atccgtacct gaatctacta
    59881 aaattgcgat tttattgttt ttcatttgtt ttctcctctt ttaaatattc ggtttctaga
    59941 acaatcgttt tttccgcaag aagtttcatt gcaaacgata aagttgccgc tcttacagca
    60001 atatattcta caggggttgc ctggtcgaat ttttgcggat gagatttacc ttgtgctaat
    60061 tggctgacca tccacacagc tgcttcttgt tcatcgcaaa atagattata ggcttcacga
    60121 attcctactg tttttccttg gaacaatatc ccttctttga tttcaggaat agtcaatact
    60181 tgtttcaacg tagtgattgc aagtaaaaat gctacgtgtt ctttgttata tcttttttta
    60241 actggtgctg gtatcaaacc tagtttaacg taattgttta ccatagaaga ggtaagcaat
    60301 ggatgctttt ctccttgtat cacaggtgaa agataacgat caactaacgt tctgacttgg
    60361 tccatataaa gttctaagtc agggagttct tcccagtgag gcagacggaa tgtttctagt
    60421 tccttccccc actccattaa ttgctgacgc attttttcca tcataaccgc tccttttttc
    60481 atttcataaa catgatatca gacttatgat aaacattcaa tctagttgct ttaactaggt
    60541 gatttgatta gcgggattcc atgaaaacaa aaaacaagaa ggctaagcct tcttgtcttt
    60601 tatcgttaag aacaggagac ccgccgggga gacggcggga aaggagtaaa aaatgaaaaa
    60661 agtgttaggg tttgttctct tgcaagaact ttgtttgttg gtatgcttat ataatagcga
    60721 gcaaatgtga ataatctatg aatgatttcc tataaggaag taaagatttt tctgattttc
    60781 tttaaatgta gtcttgcatt ttaaaattct tagtgctact attattaagt actttttgta
    60841 cgggccgtta gctcagttgg tagagcagct
//
LOCUS       DAZKCC010000007        58873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000007 DAZKCC010000000
VERSION     DAZKCC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.007"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..1445
                     /locus_tag="ACG8VH_000446"
     CDS             231..1445
                     /locus_tag="ACG8VH_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP9109035.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1476..2903
                     /locus_tag="ACG8VH_000447"
     CDS             1476..2903
                     /locus_tag="ACG8VH_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9109036.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            2926..3852
                     /gene="glsA"
                     /locus_tag="ACG8VH_000448"
     CDS             2926..3852
                     /gene="glsA"
                     /locus_tag="ACG8VH_000448"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9109037.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            4060..5520
                     /locus_tag="ACG8VH_000449"
     CDS             4060..5520
                     /locus_tag="ACG8VH_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109038.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            5741..7504
                     /locus_tag="ACG8VH_000450"
     CDS             5741..7504
                     /locus_tag="ACG8VH_000450"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9109039.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            7588..8046
                     /locus_tag="ACG8VH_000451"
     CDS             7588..8046
                     /locus_tag="ACG8VH_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9109040.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            8155..8838
                     /locus_tag="ACG8VH_000452"
     CDS             8155..8838
                     /locus_tag="ACG8VH_000452"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9109041.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            8985..9599
                     /locus_tag="ACG8VH_000453"
     CDS             8985..9599
                     /locus_tag="ACG8VH_000453"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9109042.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            9615..9821
                     /locus_tag="ACG8VH_000454"
     CDS             9615..9821
                     /locus_tag="ACG8VH_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109043.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      10046..10212
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            10360..10917
                     /locus_tag="ACG8VH_000455"
                     /pseudo
     CDS             10360..10917
                     /locus_tag="ACG8VH_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            10975..13014
                     /locus_tag="ACG8VH_000456"
     CDS             10975..13014
                     /locus_tag="ACG8VH_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9109044.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQQTLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13123..14274)
                     /locus_tag="ACG8VH_000457"
     CDS             complement(13123..14274)
                     /locus_tag="ACG8VH_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9109045.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(14428..14958)
                     /locus_tag="ACG8VH_000458"
     CDS             complement(14428..14958)
                     /locus_tag="ACG8VH_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9109046.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(15057..15407)
                     /locus_tag="ACG8VH_000459"
     CDS             complement(15057..15407)
                     /locus_tag="ACG8VH_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP9109047.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15564..17093)
                     /gene="ahpF"
                     /locus_tag="ACG8VH_000460"
     CDS             complement(15564..17093)
                     /gene="ahpF"
                     /locus_tag="ACG8VH_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9109048.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(17103..17666)
                     /gene="ahpC"
                     /locus_tag="ACG8VH_000461"
     CDS             complement(17103..17666)
                     /gene="ahpC"
                     /locus_tag="ACG8VH_000461"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9109049.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17810..18472)
                     /locus_tag="ACG8VH_000462"
     CDS             complement(17810..18472)
                     /locus_tag="ACG8VH_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9109050.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18656..20302
                     /locus_tag="ACG8VH_000463"
     CDS             18656..20302
                     /locus_tag="ACG8VH_000463"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9109051.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            20293..21375
                     /gene="uxuA"
                     /locus_tag="ACG8VH_000464"
     CDS             20293..21375
                     /gene="uxuA"
                     /locus_tag="ACG8VH_000464"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9109052.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21483..23036)
                     /locus_tag="ACG8VH_000465"
                     /pseudo
     CDS             complement(21483..23036)
                     /locus_tag="ACG8VH_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            23184..24325
                     /locus_tag="ACG8VH_000466"
                     /pseudo
     CDS             23184..24325
                     /locus_tag="ACG8VH_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(24451..25530)
                     /locus_tag="ACG8VH_000467"
     CDS             complement(24451..25530)
                     /locus_tag="ACG8VH_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9109053.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25639..26079)
                     /locus_tag="ACG8VH_000468"
     CDS             complement(25639..26079)
                     /locus_tag="ACG8VH_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9109054.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(26136..26546)
                     /locus_tag="ACG8VH_000469"
     CDS             complement(26136..26546)
                     /locus_tag="ACG8VH_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9109055.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26727..27161)
                     /locus_tag="ACG8VH_000470"
     CDS             complement(26727..27161)
                     /locus_tag="ACG8VH_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9109056.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(27261..28109)
                     /locus_tag="ACG8VH_000471"
     CDS             complement(27261..28109)
                     /locus_tag="ACG8VH_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9109057.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28498..30264
                     /locus_tag="ACG8VH_000472"
     CDS             28498..30264
                     /locus_tag="ACG8VH_000472"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9109058.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            30366..31625
                     /locus_tag="ACG8VH_000473"
     CDS             30366..31625
                     /locus_tag="ACG8VH_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9109059.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31772..33073
                     /locus_tag="ACG8VH_000474"
     CDS             31772..33073
                     /locus_tag="ACG8VH_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109060.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            33076..33927
                     /locus_tag="ACG8VH_000475"
     CDS             33076..33927
                     /locus_tag="ACG8VH_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9109061.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            33940..34755
                     /locus_tag="ACG8VH_000476"
     CDS             33940..34755
                     /locus_tag="ACG8VH_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP9109062.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            34906..36072
                     /locus_tag="ACG8VH_000477"
     CDS             34906..36072
                     /locus_tag="ACG8VH_000477"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9109063.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            36302..36949
                     /locus_tag="ACG8VH_000478"
     CDS             36302..36949
                     /locus_tag="ACG8VH_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9109064.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            37033..37977
                     /locus_tag="ACG8VH_000479"
     CDS             37033..37977
                     /locus_tag="ACG8VH_000479"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP9109065.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGV
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            38112..39563
                     /locus_tag="ACG8VH_000480"
     CDS             38112..39563
                     /locus_tag="ACG8VH_000480"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP9109066.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39584..40420
                     /locus_tag="ACG8VH_000481"
     CDS             39584..40420
                     /locus_tag="ACG8VH_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9109067.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            40420..41079
                     /locus_tag="ACG8VH_000482"
     CDS             40420..41079
                     /locus_tag="ACG8VH_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9109068.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            41205..41894
                     /locus_tag="ACG8VH_000483"
     CDS             41205..41894
                     /locus_tag="ACG8VH_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109069.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            41900..42856
                     /locus_tag="ACG8VH_000484"
     CDS             41900..42856
                     /locus_tag="ACG8VH_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP9109070.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            42900..43412
                     /locus_tag="ACG8VH_000485"
     CDS             42900..43412
                     /locus_tag="ACG8VH_000485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109071.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43668..44216)
                     /locus_tag="ACG8VH_000486"
     CDS             complement(43668..44216)
                     /locus_tag="ACG8VH_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9109072.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(44305..45486)
                     /locus_tag="ACG8VH_000487"
     CDS             complement(44305..45486)
                     /locus_tag="ACG8VH_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP9109073.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(45722..47230)
                     /locus_tag="ACG8VH_000488"
     CDS             complement(45722..47230)
                     /locus_tag="ACG8VH_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP9109074.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            47389..47580
                     /locus_tag="ACG8VH_000489"
     CDS             47389..47580
                     /locus_tag="ACG8VH_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109075.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            47783..48700
                     /locus_tag="ACG8VH_000490"
     CDS             47783..48700
                     /locus_tag="ACG8VH_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9109076.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            48693..49766
                     /locus_tag="ACG8VH_000491"
     CDS             48693..49766
                     /locus_tag="ACG8VH_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109077.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            49914..50432
                     /locus_tag="ACG8VH_000492"
     CDS             49914..50432
                     /locus_tag="ACG8VH_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109078.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            50413..50805
                     /locus_tag="ACG8VH_000493"
     CDS             50413..50805
                     /locus_tag="ACG8VH_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP9109079.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            50881..51816
                     /locus_tag="ACG8VH_000494"
     CDS             50881..51816
                     /locus_tag="ACG8VH_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9109080.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            51949..52728
                     /locus_tag="ACG8VH_000495"
     CDS             51949..52728
                     /locus_tag="ACG8VH_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9109081.1"
                     /translation="MNKKKSAPWLQALHAVMPLYISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            52721..53038
                     /locus_tag="ACG8VH_000496"
     CDS             52721..53038
                     /locus_tag="ACG8VH_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9109082.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            53282..54892
                     /locus_tag="ACG8VH_000497"
     CDS             53282..54892
                     /locus_tag="ACG8VH_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9109083.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            55049..55897
                     /locus_tag="ACG8VH_000498"
     CDS             55049..55897
                     /locus_tag="ACG8VH_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9109084.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            56128..56607
                     /locus_tag="ACG8VH_000499"
     CDS             56128..56607
                     /locus_tag="ACG8VH_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP9109085.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            56640..58868
                     /locus_tag="ACG8VH_000500"
     CDS             56640..58868
                     /locus_tag="ACG8VH_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9109086.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
BASE COUNT        19628 a        10448 c        11516 g        17281 t
ORIGIN      
        1 tgaaaaaata tctttaaaaa taaaaaaaga cgaaaataaa cggttttcta taaaaagaag
       61 accgtttatt ttttgtcttt atttgtgtga aaaacgaaaa agagcacaac acatcaagct
      121 tttattaatt gtctgtcttt tataaatgca agtattaatt caaaaaaaca gaagaaaaaa
      181 acaactacac cttcttttat actcgtaatc gtagaaagga ggcgaaaaaa atgaatacat
      241 tacctatcaa tacaggagat acagcattca tgattttatg tacagcaatg gtttgcctga
      301 tgactcctgg tctagctttt ttttacggcg gattagcacg gaagaataat atactaacga
      361 tcatgtctca atcttttctc tccgttggga taacaacgat tatgtgggta tttggtggat
      421 tcggattggc ctttggacgg gatatcggcg gtgttattgg aaacccggca gatttctttt
      481 tgatgagaca tgttacaagc ttccccaaca tgtttcatgg tgcaacgatt ccatttttaa
      541 tgttctttct ttaccaactg atgtttatcg tgattaccgt tcctttgatg acaggagctt
      601 ttgcagggag attgaattta aaaggctata ttatacttgt tattttttgg aatctgctga
      661 tttatttccc tgtttgccac tggatatggg gtggcggatt tttggatcag atgggattta
      721 gagatttcgc tggtggagca gtcatccaca cgacagctgg attcggctct ttagcatgta
      781 tcctcacact tggtcaaaga aagattcctt taggagaaag aagtcagcat agcagtctta
      841 tggcagctgc tatcggtaca ggactgcttt ggtttggctg gttcggtttt aattccggtg
      901 gtgcacttcg agcagatgaa caagcagtca atgcatttgg ttcaacattc atcgctcttg
      961 catttgcgat gattacttgg ctgatcattg caaaagtaaa aggaaatggc tttgattttg
     1021 tcgacgtact aacaggatcc gttgctggat tggcagccat tacgccttgt gccggttatg
     1081 tagaagcaaa aagtgccatg ctgattggga tcattgcggg tatcgtctgt catgctgctg
     1141 ttgattttcg taaaaagaaa caatgggatg atgcacttga tgtgtggggt gttcatggca
     1201 tgggcggatt cacaggtacg attctgatag ggatctttgc atctaacagc catttactaa
     1261 cgaatccaag cagctggtac ttcttaggga ttcaagtgat tggtgtcatc attactgccg
     1321 tgtatgccta tgtgctgaca acagtgattt taaaatcggc gatgcacttt acaacgatca
     1381 ctactacaaa agaagaacaa gaagaaggat tggacttttt cttctttggc aacaaactca
     1441 attgaaacaa attcaaatag aaaagaggaa gattcatgca acaaagcaaa acgaaaattc
     1501 ctgcaaaaaa attaacgttc tttggctttt tggctatgac catctcaatg gtcgtttcat
     1561 tatatgaata tcccacattc gcgacctcag gcttcagctt agtatttttc ttgttattag
     1621 gcggtttgct atggtttatc cctgtggcat tgtgcgcagc agagatggca acggtgaaag
     1681 gatgggaaaa aggcggtgtc tatacttggg tcagtcgcac attgggaaaa cgattcggtt
     1741 ttgccgcaat ctttttccaa tggtttgaga taacagttgg ctacttgacc atgctgtatt
     1801 tcttaactgg tgcattatcc tatgcaacgg gtattggtgc tatacaaaac aataaatatc
     1861 tgaaattggc aatcctattg attattttct gggctatttt actctcacag ttaaaaggaa
     1921 ccaaatacac ttcattgatt gctcggatcg gttttgtagc tggtatcttg ttgccagcat
     1981 tagtcttgtt tggcttaggg atccattatg tcgcttcagg cgctccacta caaacgatac
     2041 tgtctatgaa aacattgatt cctgattttt cgaaattgcc tacgctggtt gtttttgttt
     2101 cctttatttt agcatatatg ggcgtagaaa catctgccag tcatgcaaat gaaatggaaa
     2161 accctaaaaa ggattatcca ttagcaatgt ttgtattggt tattctggca atcattttag
     2221 atacttttgg cggattgacc gtcgctacta cagtacctca acatggtttg tctcttaata
     2281 caggggtgat tcaatcgtta gaattcctat taagacacat tgatccaaat cttgcatgga
     2341 ttgcgaaaat tcttgcgatt cttgtttgtt taggtgtcat cggagaaatt gcttcttggg
     2401 taacctctcc ttcaaaagca cttcacgtag cagcagaaga aggattactt cctgaatatt
     2461 ttgcaaaaga aaacacacac ggtgttcctg ctcatttgat gatcgcaaat gggatcgttg
     2521 caacaatctg ggcagccgtc tttacattaa gcggcggggg gaataatctt tcatttcttg
     2581 cagcgatgtc attgactgtt gtaatctacc tgatgttgta ctttatgttt ttcgcgggct
     2641 atttgaagct ggtttttaaa gatgacagtc ttgaaagagc ctatcaggtt ccaggcggaa
     2701 aagttgtaaa agtaatcgtt gcttgtttag gtcttttgac ttcacttttt gctttttgta
     2761 tctcatttgt tccacctgct agtatcgcag ctggacagca tgggatttat gaaacgattc
     2821 tgattgtttc ttatcttatc gtcttagtta ttccatttac gatctacgca tgccgtcata
     2881 aattccagca tgcagaaaaa taaaaaatga aagctggtta ttcaaatgga agtaaaagaa
     2941 ttagaacaat tgattggaaa aaatttccac tattatcaaa caggacaagt ggcggattac
     3001 attccagcat tagctgaagt ggatcccaaa caattaggta tggcgattta tgatttggat
     3061 acaaaacaac tgattgaggg aggagacgcc gatgtgcgct ttgccatcga aagcatgtca
     3121 aaagttcccg tattgttatt agcaatcaaa gacaacggaa tagaaaaagt ctttcaaaca
     3181 atcaacacgg aaccaaccgg ttttgccttc aattctatca tgaatatgga aatcaatcat
     3241 cgcaaacatc caatgaatcc ttttgttaat gcaggagcga ttgcaacaac ctcgttgatt
     3301 gccggtaaag atgcagatga aaaatttgag cgaatcttgt catttatgaa agagatctgt
     3361 gatgatccta atatcacatt gaatgaagaa atctatcatt ctgaatcaag aacaggtgat
     3421 atcaatcgct ctcttgctta ctatatgaaa ggtaatcaaa tgatcgaagg agacgtaccg
     3481 gagatactcg atgcttattt cagacaatgc tccctcaatg tcaccgctac aggcatagca
     3541 aaattagctg ctgtgctggc aaataaagga attgctcctt ggaatggcaa acaaatcatt
     3601 actgaagaaa gtgctacaat cgtcaaatca attatgacaa ctgctggttt gtatgatgaa
     3661 tcaggagagt tttccgtcca tgttggtgta cctgcaaaaa gcggtgtagg cggcgggttg
     3721 atggcagctg tgccaaatcg ttacggcata ggtgtcttca gcccggcact agatccattt
     3781 ggtaacagcg cagctggtat ccaattgttg aaagatgtag tcaaagaact agacgctgac
     3841 atctttgaat aaccattctt gaaagaggct ggggtaaaag tccaaacggc tcaaagctac
     3901 ctacaatgag gctgaaattg tgagcttgtt tgacttttgt cccagtttct ttttttattg
     3961 gtttttgaaa gggcttttct aaataaaaag gaaacaaaaa tacgatttac agatatgatt
     4021 aaataaaatc ataagaaaaa ctggaggcgt gtatcaggaa tgctggaaaa ttacatcgag
     4081 cgaaatatat ttagaaaagt gtatctgtgt gaacaattat ttgaattcca agaaatagac
     4141 atcgaacaaa cagctatttc tctaagggtg actacaccaa cgatccttca cgatctagaa
     4201 agtctagccg agtgtctaga atattgtata aaagaacaag taagagagaa gcataagtat
     4261 aaactcgtat tcaaacatgg tattgctctt tctgagctca cacagtttct gtacggtcaa
     4321 tcttattttt taaagttttt aagttattat tttaatggta tattcacgag tacagaactg
     4381 gctgatcttg aatttatttc tttgagcaaa gtatatacaa tcaaaaaaat cgtattggat
     4441 ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga atttgactcg
     4501 cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga aaataaaaat
     4561 ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca tttttttaaa
     4621 agaagatatt cggaagatga aaagtccttt atcatacgag gaatagagat cgcaatcgga
     4681 agaaaggcat ttccccttgg actgaaagaa gaagataaga aaagcgcaga aacgaagccg
     4741 ttatttcaat tagtctgtca aggattggaa gagcaaaaag gaattctaga cttacaagaa
     4801 gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa tgagaacatg
     4861 gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa tgacctatca
     4921 ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc agaaaatttt
     4981 attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg acaagtattt
     5041 ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg aaaaattgta
     5101 gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa tgaaaatttc
     5161 attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg gagacaggaa
     5221 atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg aacccaattt
     5281 catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg ttcattagaa
     5341 gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga tgctgctagc
     5401 tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt aaaaggtgca
     5461 aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca tatttcttga
     5521 tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga aggtttttat
     5581 ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga aattttttta
     5641 aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt aacaaatcta
     5701 ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag tattacatgt
     5761 attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg aaatgaaaca
     5821 gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag gtgggcacgt
     5881 tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat ttgattcacc
     5941 aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc ttttaactgg
     6001 tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt ccaaccaaca
     6061 tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca gcttagcaac
     6121 aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag catttatcgg
     6181 tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg gtgttttaca
     6241 atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga atcatggcag
     6301 tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca ataacctatt
     6361 taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg aaacggtgat
     6421 ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg tccatacgga
     6481 aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt ggcatgtacc
     6541 atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat ttaatatgat
     6601 catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg ccgcagtcaa
     6661 tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc cagatcatta
     6721 ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt tggcttctca
     6781 gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat atgatcaatt
     6841 atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg gcggaacgat
     6901 ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat caagtatccc
     6961 gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc tagattgggc
     7021 gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg tagaacatgg
     7081 tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata atggaggaaa
     7141 agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag taaaagcttt
     7201 tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag aaaatgacgt
     7261 agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag aagatggtag
     7321 tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt caatgaacgt
     7381 attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc agctttatga
     7441 acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct tgaatattca
     7501 ataattcatt atgctcacca tccagataat tgtttatact agtatctgga tggttttttc
     7561 attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata tgtcaaaaag
     7621 ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc atttggggat
     7681 acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa gactggtaca
     7741 tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt tggtcaatta
     7801 gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat aaatgtgacc
     7861 atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga aggggatcgg
     7921 acattagctt attggcggaa agtccatgct gagtttttta aacatgctta taaagaagcg
     7981 ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga agtaattttc
     8041 ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga atagaaaagg
     8101 gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga tattgtggat
     8161 acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga aaacgagccg
     8221 ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg cggagcagtt
     8281 ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt tgatttaccg
     8341 gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac tgcgacagaa
     8401 aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga tgcaacttct
     8461 cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca cgaacacggt
     8521 cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca agaagatggt
     8581 tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa gacgcagaca
     8641 cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt aattatggaa
     8701 ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc tttttcagtc
     8761 gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt cgaagccgca
     8821 agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa agaaaaattg
     8881 atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc attttgtctt
     8941 caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt gaacggagtc
     9001 cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc atccctagac
     9061 cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca cccttcagtt
     9121 acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag agaaaacaag
     9181 atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt atccatatcc
     9241 ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct aatgaggaaa
     9301 gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca gtaccagcat
     9361 tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag atacggaata
     9421 aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat ttaaatgaaa
     9481 cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct gtaagccgtt
     9541 tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt cccgaataaa
     9601 aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca aaagaaatga
     9661 aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa gttccgccat
     9721 tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata gaaatgccag
     9781 atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt ccgaaaagtg
     9841 aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct ggtgcaactg
     9901 aaagaatttg caacgggatt ctttcaactt atttgttatt tttttctaaa aaatattaaa
     9961 gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag gaaaagtcta
    10021 tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc tatacaaaca
    10081 taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac tgttggatta
    10141 aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa aactcggacg
    10201 atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt tctttatatt
    10261 tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt ggattgctgt
    10321 gtgaggagga gatgcggtga aaaaagatcg attggtggag gtcgctcgga ttcacttctg
    10381 gttgcacaat acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca
    10441 tatgtcgata ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga
    10501 gttgatttat taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca
    10561 agtatcacat ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag
    10621 atcgatgcag aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg
    10681 atggatggta atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc
    10741 gggaggatca gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta
    10801 acttcaatat tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac
    10861 cttccagaaa gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat
    10921 aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa
    10981 agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta
    11041 atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta
    11101 gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac
    11161 aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag
    11221 atcttagata aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg
    11281 aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg
    11341 aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat
    11401 ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac
    11461 aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa
    11521 ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat
    11581 ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat
    11641 gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa
    11701 atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc
    11761 agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca
    11821 gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt
    11881 ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca
    11941 gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat
    12001 atcaaattga caatcgatgc gaaggcacaa aaaatagctt ttgacagtct aggaggaaaa
    12061 gctgcatcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct
    12121 ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat
    12181 gaagaaaatc ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct
    12241 acgtttaaaa tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa
    12301 gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc ttttaaaaca
    12361 acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat
    12421 aatatatatg cggcacaaca aacgttgaaa atgggtgaga aaaaatttcg tacaggctta
    12481 gataaattca tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt
    12541 tctaatgaag atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc
    12601 gaacttctaa ttactcctat ccagcaagca gcaatgtatt ctgtttttat caacaatggc
    12661 acacttgtct atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc
    12721 agcgaaacag cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat
    12781 ggtacagcac attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg
    12841 gaaatcaaag aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat
    12901 cttgataacc aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca
    12961 gcaaccaaac gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt
    13021 gctttttaag tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa
    13081 cggttttcac gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt
    13141 ttttgtaaaa tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac
    13201 taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat
    13261 caaagccatt tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga
    13321 aaaaccggca atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag
    13381 cgtcaatata gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc
    13441 tacactgaca aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta
    13501 tacttcttct ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac
    13561 agagctaagc ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac
    13621 gatgataata gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat
    13681 ccatttgact aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg
    13741 taaagaagca ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa
    13801 aacgactaaa atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt
    13861 cactacgttt agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat
    13921 aatcccaaag aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc
    13981 aaaaacaacc gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt
    14041 tttcaaaaga gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat
    14101 ctccgaaaag tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag
    14161 caaaacacca acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg
    14221 tgagccaatc gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat
    14281 cattcctttg tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg
    14341 aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat
    14401 acggtctatt ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa
    14461 attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag
    14521 catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact
    14581 actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct
    14641 gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa
    14701 agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca
    14761 agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac
    14821 aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat
    14881 cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg
    14941 tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca
    15001 ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa
    15061 cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa
    15121 taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca
    15181 cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa
    15241 gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa
    15301 atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata
    15361 ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt
    15421 tctttttttc aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt
    15481 attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc
    15541 tgacttttta caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct
    15601 gcagtagctc ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca
    15661 ccagctgcga aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt
    15721 cttggattca tttcgatcgt tctatctagc caatccgtat tagggagtaa gccgactaga
    15781 atgaatactc ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag
    15841 agagattcta cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg
    15901 acattcgcaa gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag
    15961 aattctagaa catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca
    16021 gaattaccac caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt
    16081 gggcaataag caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc
    16141 cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag
    16201 ccaatattaa taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg
    16261 tcgatagggt aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg
    16321 taagaggtac caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa
    16381 tcagcgacaa gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct
    16441 gctggtccac cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca
    16501 atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat
    16561 tcttctccat ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt
    16621 tggaacatcc caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata
    16681 ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa
    16741 tgcaatgttt gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc
    16801 cgtccactaa tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata
    16861 ccagcaaaaa cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc
    16921 atcgaatctc ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat
    16981 tcagctagtt tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat
    17041 tcaatcagtg ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc
    17101 acctaaatct ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat
    17161 tttgccggac aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca
    17221 atcagtgcac tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt
    17281 ccgtctggat caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca
    17341 tcaaaaatac gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg
    17401 atagcatctg atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa
    17461 aagacttcaa cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca
    17521 gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata
    17581 agatcttttt cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct
    17641 tcgatttttt tcccaatcaa tgacatagta aaacctccaa taataattat ttgtttataa
    17701 ttattgtaat ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta
    17761 ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga
    17821 aatactcggg ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca
    17881 atcccgtttg aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat
    17941 aatgttgttc catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga
    18001 aacgttctaa atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa
    18061 tttcgaaaca agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta
    18121 ttttgactgc tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt
    18181 ccaatttgga acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag
    18241 cattttctgc agaaacaaga ttaattttgg aaacatacgt accactttga ggtatcgtat
    18301 acacgagttc ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag
    18361 caagatgttc ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga
    18421 taatttgttt acgaatcgct tgataagctt gtgattgcaa gctgcttttc atctttattc
    18481 ctccaaaatc atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat
    18541 gtcacattaa ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta
    18601 ttgcataaaa ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat
    18661 tgatttaaac aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc
    18721 tcattttgac cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt
    18781 cggtggcgga aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct
    18841 aggcgaattg tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag
    18901 ggcttaccaa gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt
    18961 cgtcaaacgt atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga
    19021 acactttgat aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc
    19081 tattactgaa aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc
    19141 agtcgatata aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgtgct
    19201 actttataaa cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt
    19261 ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa
    19321 ggaaggttat gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt
    19381 tccatggacg atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt
    19441 ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc
    19501 tccatttgtc aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg
    19561 acgtccgaaa ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc
    19621 agatacgatg aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg
    19681 ttgtttactg gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct
    19741 agtttctgaa atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga
    19801 tccaaagatc tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctaa
    19861 tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac
    19921 gattaaaaaa tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt
    19981 gactctcgct gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat
    20041 cccgagtcca gacccactat tgacagacct tcagcataag ctttccgata ttcaactggg
    20101 tacgcaagat acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat
    20161 ttttggtgta gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa
    20221 gatggtagca ggaccaaatg ccgtacgaaa aacattacag gattacttac aagaacatgg
    20281 aggaaattta tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc
    20341 ttagatgaaa tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata
    20401 ccagtaggag aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct
    20461 cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga
    20521 ttaccttccc gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa
    20581 tatggaatag aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac
    20641 cttgactacc gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca
    20701 gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg
    20761 ggatgggaac ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta
    20821 gacgaagaaa aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt
    20881 gaagaggtag gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga
    20941 ttagctcgaa tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc
    21001 ccttataacg gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta
    21061 tatgggattt tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat
    21121 attaaatttt tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca
    21181 ttagacatgt accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt
    21241 cctgatcatg gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat
    21301 cgagcattag gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa
    21361 agtaaagata attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa
    21421 attcttgata cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg
    21481 gtctaggaag taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat
    21541 tcaaagatat attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt
    21601 ccttttaaaa tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg
    21661 ataacgatgt tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa
    21721 atttgttcta tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca
    21781 ccaatcacaa ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc
    21841 atccattgca cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg
    21901 aaatccatat aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga
    21961 taaagcatga aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa
    22021 agtttatttc gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa
    22081 tcaaatacag tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt
    22141 gcttttttta aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata
    22201 gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt
    22261 cgcaaaatat ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct
    22321 ttttgtacgt ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa
    22381 tataatttat ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta
    22441 ttcatcataa aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa
    22501 aagatgttcc aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa
    22561 gactttctgc cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta
    22621 atctttttca ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg
    22681 ataatcgaat ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg
    22741 ttgcagtatc ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc
    22801 aactttaaaa aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag
    22861 tcttgttcta attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgatct
    22921 gctaaatatt gtgtagtaat aaagaaacga tgttcagata ggaaaagaaa aatatctaat
    22981 tttgtattgc tttttttctt caagataaac gttttaatcg cttcttgagt ttgcatgaaa
    23041 gaggctcctt tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt
    23101 tagacaaaaa aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa
    23161 aaaaatcaag gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag
    23221 ccttaagctt atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca
    23281 aatggacaga aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat
    23341 ccttcaattc aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa
    23401 ttgactattc gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg
    23461 atcccacttt gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga
    23521 tatttacctt caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca
    23581 aggtatcaga tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa
    23641 ttgagcaaga ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac
    23701 cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac
    23761 cggaaataga acctggagaa gaacccgata cagaaactca accagagcca gacaataaat
    23821 caaaacctga aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt
    23881 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
    23941 aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg
    24001 caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa
    24061 aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa
    24121 aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa
    24181 cctttgctgt cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa
    24241 agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag
    24301 atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat
    24361 aaaaagaaat atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc
    24421 gttcatactt tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg
    24481 gttcgggatc tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac
    24541 aatcaagccc caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa
    24601 aatcggcagt tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag
    24661 atggacaatc aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata
    24721 agctatgaaa gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa
    24781 aatgaaaatc atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat
    24841 tgtagtgagc aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa
    24901 tgtattgaca aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact
    24961 ttttaaaaac aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact
    25021 taagatgaat gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag
    25081 cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg
    25141 gatatattgt aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat caataacgga
    25201 ctggatcata tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata
    25261 gataaggtaa acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac
    25321 aaatcgctga atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa
    25381 aatggttgta atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt
    25441 gagacggcgt attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc
    25501 caaataacaa aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag
    25561 aaagagcaaa aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa
    25621 atcgttcatc tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt
    25681 tcgggtcaga tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat
    25741 cttttgtgga ataccttctg cttgtttttg caacgcagca ccttgaggag tcagcgtaac
    25801 atttacgatt ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa
    25861 cttttttagt aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt
    25921 cactgaagtc tttttctttt cccataaaac taacatcaca agatactggg tataggtaag
    25981 attcagcggc ttgaggaggg aagtgtagtg tctaatgatt tccttagagg cggcatataa
    26041 aggaaaacat acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta
    26101 ctatagtgta tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta
    26161 acttctatat ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat
    26221 aattcttgcg tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag
    26281 acaaagctcg tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca
    26341 gagatatttt tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca
    26401 gctgcaaaca attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata
    26461 ttcaattcga atgtacgatc gggattatga acttctccag agcgtccgcc tgtgttgatc
    26521 attttcgtag aatacatttt tttcataaaa ttaccacctt taattatatt ttatacaatt
    26581 gaattttaca caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga
    26641 gaaagcacat gaaacagacg cgctttctcg aatagcaaac atatacaaaa aaatatcaga
    26701 gacaaaaact aatctcatca taaacattac atatatttct caaaaagttg ttttctttct
    26761 tcaaaactta cttcacgatg gacctgatga agcatccata aataaccaaa aggatctctg
    26821 aacattgagt tagtcacacc aaagtccatc atttctgtca tttcttgaat ctctacacag
    26881 ccatttcgta atgctttatt atgtgtgtcc tcgatatcag agacgatgat attgaaccag
    26941 atcgtatttg gatgttcagc agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc
    27001 atatggaatc tcgtgccata caaggtaaaa atgacttcat tattgcctct agggtaatct
    27061 gttgcctcaa tcagttgtat ttcatcaaag attgtttgat acagttctag tgctttcaaa
    27121 ctatcattta atacaaaatc tatttccgtt cctttatgca taaagatctc ctctcctttt
    27181 cagtcgaaca gttactagat tcttttatta taccagttaa agaaaacgca ttcaaaaaaa
    27241 tagcggattt acttaccaac tcattcgatt ggatgataag taaatccgcg tcccattact
    27301 tctgctacgc tgctgacaat gacaaaggca cttggatcga tttcttctat caatggtttc
    27361 attcgggaaa attcatgatc agtcacaacg atcaataaaa cctcttttga ctttcgatta
    27421 tacccgcctt ttgcctctag cagggtaagg ccacgtccaa tctcagcact taagcgaagg
    27481 cggatgtctt ccaaataatg ttcactcatg accataacag atttctttcg attgatacct
    27541 gtttcgatat aagtcatgac aattgaagtg attgcaatag ataaaatagc ataaagaaac
    27601 tcttctattc caaaaacaaa cagattaaga gaaacaacga atgcgtctgt aaaaaataag
    27661 ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg
    27721 ccgctagaag agttgttttt atataaaatc gcaactccta aagcaaaaat cgcacttcca
    27781 aaaatgacag acaacaaccg atctgaagta atcattgttt ctggaactat tgcaatgatt
    27841 aaaggaaaaa acaagcttcc gaaaagtact ttaaaaaagg gttttttacc taaaaataga
    27901 agagctagta ccaacataac gatattcaac actaaaataa ctatagctcg gtcaaatcct
    27961 aaaacagctt ccagtaatat tcctagaccg ctgacacctc cagcagcaat atgatgaggc
    28021 cctaaaaaca tattgatagc gactcctaat atgaagaggg caaaaataat cttaatgtat
    28081 gtccatataa attgtctcgt ttctttcatt tatccacttc cttaattcat taataatact
    28141 cttatcttat tataacttac ttttcacctt aagttaactt taattaataa aaaaattagg
    28201 ctagtctaaa ttattatgtt tcattcttct agaatatact tctacgttaa aaactgttaa
    28261 aaaactttta ctttagaaaa ttggagaaat tgatcgctta agataaattc aggacactaa
    28321 atagtacagg aagtagatta aaacttttaa aagttttaag tctatctatt tggcaagttt
    28381 aaaaaataaa aattcggtat caaaaaactg ttaccgataa catttttgaa agcgttgtct
    28441 tttgtttctc tatcacttat agtgatgacg tcatattaaa acacaaggag gaaatcaatg
    28501 gatacagctg caatttatca tcgaccggaa agtgaatatg catttctata tacgaataca
    28561 agatttcgta ttcggcttcg tactaggaaa aatgatatca aaaaagtata tgtgctttgt
    28621 ggagatccat ataccataac aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa
    28681 tgtctaagta ccaatgttca tgattattgg gaaattgaag tgacgagcga aacaaggcgc
    28741 ttacagtatg cgttccatgt tgttggggaa gataacacgg actgttttta cggtgatcaa
    28801 ggaatttttc cttatcgaga aaatgtgatt actgaaccaa atttttattt tcgaatacct
    28861 tattttcatc aaatcgatcg attcactatt ccagaatggg taaaagaaac agtatggtat
    28921 caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa atgaccccga acacacttta
    28981 ccttggggaa gtaaagatcc agaccgagaa gacttcttcg ggggagattt gcaaggtgtg
    29041 agggatcatt tggattactt gacggacctt ggcgtcaatg gcatctattt ttgtcccata
    29101 tttaaagcca catccaatca taaatacgat acgattgatt attatgaggt ggatcccgct
    29161 tttggcgata aaaaactttt aaaaaacttg attgatgaag cacataaaag agggatacgt
    29221 atcatgttcg atgctgtatt caatcatatg ggtgtccatt caccacagtg gcaagacgtt
    29281 ctcaaaaacg gagaaaaatc gatctataaa gactggttcc acatccgttt tttcccagta
    29341 gattcttatc agatgactga cttaccagaa actgctgaaa atatcccata tgatactttt
    29401 gcttttactc cattcatgcc gaaactaaat acagcaaatc cagaagtgca aaaatatttg
    29461 ctggatatcg caacttattg gataaaagaa ttcgatattg atggatggcg tcttgatgtt
    29521 gctaatgaag tcgatcatca tttttggaaa aaattcagag aagcagttac agaaatcaaa
    29581 ccagacatct atatccttgg agagatttgg cattcctctc aagcttggct gcaaggggat
    29641 gaattccatg cagtgatgaa ttatgcattc acagattcga ttaaagatta ttttgcaaaa
    29701 aagaaaatca cagcttcaca aatggtcagt ggaatgaatc atcagcaaat gctttacaga
    29761 gatcaggtga acgaaggaac attcaactta ttagattctc atgatactgc caggatcttg
    29821 accctttgcc aaggtaacaa agaattgatg aagtctgtga tggcctttat gtttcttcaa
    29881 aaagggtctc cttgtatcta ttacgggaca gaaataggga tgactggtga agatgacccg
    29941 gattgccgca aatgtatgat atgggagaag gaggaacaag atctagagct attcggtttt
    30001 ataaaagaac ttgtttctct aaggaaacag ttgtctaaga ttatttctga aggttctact
    30061 cagtggctaa tagtatatga tcgtgaagat aagctctatt tcacacggga actagaggga
    30121 cagatcatct atgtttactt taatcagtca aaagaacctt gggtagtaga acaggaaaat
    30181 gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa
    30241 tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa
    30301 atgaagattt gtgcccatta taataagggt gtaacagcac gttgaataca caggaggaaa
    30361 tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg gttaatctct ttaggaatct
    30421 taggcggatt agcggcttgt ggcaatggta actcttcagc agaaggggaa gccgatgaag
    30481 gaaaaacgtt aacagtttcc gttgattctg gatataaaga ttatataaac gaaataaaaa
    30541 acactttcga aaaagaaaac gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc
    30601 agttggaatc attagcgtta gatgggcctg ctggaaaggg acctgacgtt atgatggcag
    30661 catatgaccg gatcggtgcg ttaggacagc aaggacatct ggcggaagta aaactgggaa
    30721 atgaagcagc ttacgatgaa acagacaaag cccaagtgac ttatgatgga aaaatctacg
    30781 gagaacctgc agtaatcgag acccttgttt tgtactataa caaagattta gtcgatacag
    30841 caccatcaac gtttaaagat ttggagaatc tatccaaaga cagtcgtttt gcttttgagt
    30901 cagaagcagg aaaaaataca ggatttttag ctaaatggac tgacttttat tattcctatg
    30961 gtttaattgc aggttacggt ggctatgtat ttggtgatga tggcacaaat ccttcagata
    31021 tcggtttgaa taatgcaggt gctgtagaag gaatctctta tgcaacagat tggttcaaaa
    31081 atgtttggcc aaagggaatg caagacataa aaagtgcagg agacttcgcc agccagcaat
    31141 tcatgtccaa taaaactgca gcaatcattg acggtccatg gcaagcgcaa acatacaaag
    31201 aaaacaatat caattatggt gtagccaaaa ttcctactct aaataatgga cagccttatc
    31261 aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta tgctaaaaac aaagacctct
    31321 ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg
    31381 taaatgaaat ccctgcaaac caacaagcta gggaaacagc aaaggctaaa aacgatgaat
    31441 taacaacagc tgtgattgaa caatatgaaa cagctcaagc gatgccaaac atcccagaaa
    31501 tgggcgaagt ttggacaggt gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa
    31561 caccaaaaga atccgcagat gaagcagtaa aaacaatcag tgaagccata gaacaaaaat
    31621 actgattaaa atgataaccg tccattttta taaaaaatgg aacaggtgct tcagataaaa
    31681 gtggcaagag cgagatggaa aattatcttg ctcttgttgc cattatctta aaagcagttt
    31741 gcggtttagc aagtttagag ggggcattca gatgcagcaa gataaaacaa gcaatatcaa
    31801 cgaaaaaaaa atcacgcttt gttcacttat tccagggatg gggcagtttg taaatggcca
    31861 agcttttaaa ggtatcgttt ttttcgccat attcgtccta ttcattggtc agatgcttct
    31921 gggcggattt catgcattgg taggattggt tactttggga agtataccaa tggaagatca
    31981 ttcattattt attttgattg aaggcacttt gcaattcatc attacaatca ttttcttgat
    32041 tttttacggt ttgaatcttt tagacgctta tcgagtagcg aagatgcgtc aagagggcaa
    32101 aaaaatcaat cggacgatga aggaagtcat acataatata ggtgatgaag gttttcctta
    32161 tctattgaca cttcccgctt atcttctcat gatttttacg atcattttcc cagtcctagt
    32221 tacattgttc actgcattta ctaattatga cttcaatcat attccaccag ccagtttaat
    32281 tgattgggta ggggctaaaa acttttttag tatcttcttt ttaagctcat atcgtaatac
    32341 tttttttgca gtcttttcgt ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat
    32401 cacccttggt attctcacag cggttgttgc tcaccagaaa tttattaaat tcaaacgtat
    32461 tttcggtatc attttcttat taccatgggc ggtcccagct tttattacta tcatgagttt
    32521 ttcgaatata ttcaacgata gcgtaggtgc catcaatact caagtgattc ctttcataaa
    32581 ccagcttcct tttgtagata tcagtgcaat ctcttggaaa acggacccga actggacgaa
    32641 agtagcaatt atcttagtgc agggctggtt aggatttccc tatatctatg tgatggttac
    32701 aggaatctta caatcgattt cagaagactt atatgaagca gcaaaaatcg acggagcaaa
    32761 tgcatggcag cgcttttcaa acatcacgct tcctgctgtc ttcctagtgg cagcgccaac
    32821 atttattaca cagtatacgg ggaatttcaa taatttctcc atgatctatc tgtttaacaa
    32881 tggtggtccc ggaagcgtag gcggaggagc tggttctact gatattctta tttcctggat
    32941 ctataaactt acgactggaa cttctccgca gtattctatg gcttcagcaa tcacgttgat
    33001 tatctccgtc atcgttatct gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa
    33061 tatgggggat taacaatgaa aaaatttcat tcgagtgctc gattccaaaa acgactttct
    33121 cactttttta cttacttttt tcttttaata ctgtcagtca ttatcatcta tccgcttttg
    33181 atcacagcaa gtactgcctt caagtcagga aatattgcag cttttagttt agatctttca
    33241 agtgactgga ctttaggaaa ctttactcgg ctattcagtg aaacacttta cgggacttgg
    33301 tataaaaata cattgatcat ttctgtccta acaatgatcg tacaagtgac tgtagtaact
    33361 ttggcgggct atacttatag tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc
    33421 tttttagtga ttcagatggt accgactatg gcagcattga ctgcctttta tgtcatggct
    33481 ttcttattag aagcgctcga taagtactgg ttcttgacaa tgatctatat aggcggtgga
    33541 attccaatga atacttggct gatgaaagga tactttgaca ctgtaccaat agatttagat
    33601 gaatctgcta aacttgatgg ggcagggcat ttcaggattt ttttccagat tgtattaccg
    33661 cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt ttatgggacc ttttggggat
    33721 ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc agactgttgc tgtcggctta
    33781 caaacattta tttctgatgt gacaaaccaa agagtctctc tttttgctgc tggggcaatt
    33841 ttgattgcac taccgatttg tctattattc ttctttttac aaaagaactt tgttaccggc
    33901 ctgacatctg gcggaacaaa aggataggag cagagtatca tgcaaacatt tccgttaaac
    33961 tatttttccg cattacgtac gccaactcaa gtgtttgcgg gacgcaaact attatcatgg
    34021 cctaaatttt tcttgatatt tgtattttta gtttccttga tggtcatgcc tgtgactctt
    34081 ttttatgcca atcagatcca agcgattcca ttagaacaat ttctatccgt tcattcgctg
    34141 atcgatgaac aaggaaccca aaaattttct gaacttgaat tatctgaaac tgggttacaa
    34201 gcatcgcaac aaacgatagc tgtgacccct gaaattttgg taggtgtgtc tttatctgag
    34261 aaacaacaat ccgatcatgg aacttttatt gattttgaga aggaacaatg ggtgatccaa
    34321 caaaaggata aatctggtat acgccgctat acgatgaatt atagcccatc tttccaacca
    34381 gattctgtac gtacgcccga agatttccaa cgatttttag aaagagaatt ttatgccagt
    34441 aatcgtccaa caatcatttt atcttatagt ttaagtcttg gtcttgtcct atttgtcatg
    34501 acgagtctca ttttattcgg ggcttccttt ttcttgtgga tgacaagaaa aagccaactt
    34561 tcatccattc atacattcaa agagagtgca aatttgatgc tgaatgttat gggaataggt
    34621 tccatggcag cagcagttgt agggtttatc cactttgact ttatcttgat gctaagtgtc
    34681 caaacaacga taactgtctt gctgctttta tgggtttttg tcaaaacaag attcaaagat
    34741 aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa
    34801 aataaagtag tttttgaaaa atttctgaaa aaattaaact tcttagttga ttcttttctt
    34861 ttttagtatt aacgtaatag ggtagttttt ttaaggagga aaatcatgtt agagaaaact
    34921 ttatacaatc aattattttc acgttctttc tcgctccctg tcgaagtaaa ttattgggac
    34981 ggtacaacga aacaatacgg agatacggac actcctcccc aaatcaaaat caatatccat
    35041 gaagaaatcc caatgaaaga gattacaagt aacgcttctt tggcactagg agaagcctat
    35101 atggatcatc gaatcgagat cgagggaagt atccaagcat tgatcaacga tgcttatcag
    35161 caagcaaaca gctttttacg aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa
    35221 catacgaaac aaaaaaacaa agaagatatc catgcgcatt atgacttagg taacgacttt
    35281 tataaacttt ggctagatcc aactttgact tattcttgtg cttatttcaa tacacctgaa
    35341 gatacgttgg aacaagcaca aatcaataaa gtccatcata tcttagacaa actctttatc
    35401 aaagaaggcg aaacgttgct tgatatcggt tgtggctggg gaacattgat gttcactgct
    35461 accaaagagt ataatgtcaa ggcaactggg atcacactaa gtgaagaaca atacgatttg
    35521 attaccaaaa gaataaaaga agaacattta gaagataaat gccgtgtatt attaatggat
    35581 taccgcgaac taaaaggcga aacgtttgat catatcacaa gtgtaggtat gttcgaacat
    35641 gtaggttctg aaaatcttga aggttatttc aaagtggtaa aagatttatt gaaaccaaaa
    35701 ggaacagctt tgatccatgg aatcagccga caacaaggcg gtgcgacaaa tgcttggatc
    35761 aataaatata ttttccctgg cggttatgtc cctggtgtta ctgaattagt tgataatatt
    35821 acaaaaaacg atttgcaact gattgatttg gaaagcttac gtcgtgacta ccaattgaca
    35881 ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt
    35941 gattccttct atcgaatgtg ggatctatat ttacaagctt gtgcagcttc atttgaatca
    36001 agtaatatcg atgtgattca atatttgctt gttcaacctg gaaacaatga catcccaatg
    36061 catcgtgcat aattgaggca aaagtactgt ttaaagacta tttaaaaaga agctaagttt
    36121 tatgatgctc cagaagtgaa cttttttgtc taacttctag gattgtctct aaagcttagt
    36181 ttctttttct ttatattttc ataataaaag tttggttctt taatttttaa acatgacaga
    36241 aaattttttc ttcgttatac ttgtaataga atactatttt ataacaaagg ggatcgaatc
    36301 aatgaaaaaa actgttattt atactagttt atttgcagct ttgtttttag ctgcttgtca
    36361 atcaaatacc aatcaaggca catctgggaa tacgtcttcc agtagcgaac agcgctcttc
    36421 acaggtatcg gactcctcaa caaaagcaag cacatcttcg tctagccaga caaaaagttc
    36481 tagtgaagca agtactcagt cagatatcaa agtgtctgca gctcgtgcga tcgaattgtt
    36541 ccaagataaa tacccagata ctgcaattac tagtttggaa ttagatagtg attggggatc
    36601 ttacttctac aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa gaattgatgc
    36661 tcaaaatgaa aaaatggaga cagagaatcc agaaactcta gatcgagatg aacaaaatgg
    36721 tcaaaagaaa aaagaagatg gtctggatgt tgataaattg atcacgattg aggaagcagc
    36781 aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca gattgggatt tggataaaga
    36841 tttaggtact acttattggg aagtcaaagt aaaagacggc aatcaaagta catcagtcaa
    36901 gatcaatagc caaacaggcg aagtactgtc aactgaacaa gatgattaag aatttcttcg
    36961 gaaaacaaga gttgattttt cttttagtca tgatacacta gacaaaataa cttgtttgaa
    37021 ggagcgattt acttgagttt agaaacgacc tttgccaatc atatctttgc caatcctttg
    37081 atgaatgcat ctggtgtcca ctgcatgact acccaggaat tagatgaact ggctcattca
    37141 gaagcaggag catttatcac taagagctgt acgatcaatg aacgaaaagg aaatcccgaa
    37201 ccacgctatt ttgatgttcc tttagggagt atcaattcta tgggacttcc aaacttaggt
    37261 ttttcttatt atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa ccaaaatcaa
    37321 ccgttatttt tttccattgc cgggatgagt gtccaggaaa acttggaaat gcttgagaaa
    37381 atagaaaaaa gtgggtttaa tgggatcact gaattaaatt tatcttgtcc caatgtgcct
    37441 ggaaaaccac aactagctta cgattttgag gccacttatg aaacactgaa agaagtattc
    37501 agcatctttt ctaagccgct aggaatcaaa ttgcctcctt attttgattt tgctcatttt
    37561 gatcagatgg ctgatatctt gaatcagttt ccattgacct atgtcaatgc tatcaacagt
    37621 gtgggcaatg gtttgtatat cgatacagaa caagaagcag tagtcatcaa accaaaagaa
    37681 ggggtcggcg gcattggcgg agagtatatc aagccgactg ctttggcgaa tgttcgcgct
    37741 ttttatacac gtttgaaacc agaaatccaa atcattggta caggtgggat tcggacagga
    37801 caagatgcat ttgaacatct gctatgtggc gcaagcatgt tacagattgg tacagagctt
    37861 cataaagaag ggccggaaat tttttctaga atcataaaag aattaacgca gattatgtca
    37921 gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat tacgcaccat ttcatagcaa
    37981 agagacgaag gtcatgattg atcttcgtct ttttttataa aaaacactcg tctgttcatt
    38041 cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg ggaaaataac agaggcaaaa
    38101 aggaggataa tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa aaagaattta
    38161 gttttcaaga actagttacg gatatacaga aaaaaataaa gaagcaaaat catgaattga
    38221 atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca cgagctattg attccccagt
    38281 cagaaaaagg gccattggct ggtatcccat ttcctttaaa aatgctcggt caagaaaaaa
    38341 agggatggct agctacttca ggatctcgtt tatttgaaac acaccgagct tctcatacat
    38401 ctaattatgt gcggcaagca gaagcgatag gtctagttcc ttttggacaa accaatgcgc
    38461 cagagtttgg tttcaaaaat attacagacc cagtcatcta tggcccagca agaaaccctt
    38521 ggaaccttga atgtacaccg ggcggatcaa gtggaggagc tgctgctgca gtttcatcag
    38581 gaatcgttcc tttagctggt gctagtgatg gcggtggttc tattagaata cccgcttcgt
    38641 tttgcggatt gattgggctg aaaccttcgc gagggacgat gccagttggt ccatatgctt
    38701 ggagaggatg gcaaggagct gctattgatt tcggcttgac agtttctatg agagacacgg
    38761 aggctttatt ttatgggatg agaagtatcc attccggtgc gccatatcaa gtatcgccag
    38821 ttgagtggca aacacatcca agaaaaaaac ggttgaagat agctttttgt gttgattcac
    38881 cgatccactc acagattagt gaagaggcag tacttgctgt aaaaaaagct gtagatttcc
    38941 ttggaaaaga agggcatgat ttgacagaga ttccttaccc actagatggt cgtaaactga
    39001 tcacgtctta ttatcagatg aatggcgcag aaacagcagc aatgatccgc tcgattgaaa
    39061 caggcatcca gcgagctgtc agaaaagaag aacttgaacc catttcttgg actttgttcc
    39121 aatatggtca gaagctctct gccgctactt acatcgattc tctgcatgta tgggatgaag
    39181 cagcaattgt catggaaaac ctacatcaag aatacgatct attcctttca ccaacgactg
    39241 catttaccgc ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga gcacagatgg
    39301 aacacatcag tgattgttct gaagtagaag gtttagacgt tgtttcttcc atgtttgagg
    39361 agagcttgaa gatcacacct tatacacagt tggctaatct gactggacaa ccagcaatca
    39421 gcttgcctac tcatttgaca aaagaaaaca tgcctctagg aatccaattt atggctgcca
    39481 gaggaagaga agatttgcta tttcaaatcg gctatctatt tgaagcaaat catcagttcc
    39541 atttaccaaa tccatggaaa tagcagaaac aaggagaaga agaatgaaga aaaaaagatt
    39601 gtttgctcta ataactagtg ccttttttat tttaagcgct tgcggtacag ctgccgctaa
    39661 tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa gaattagtag tagcgacttc
    39721 tggcacactg tttcccgcat cttattataa tgatcaaaat gaattagttg gttatgatgt
    39781 agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta aagaatacaa
    39841 cgtggatgga caaatcactt ctgtttcaaa aagagaatca gattttgctg cgaacgattt
    39901 tggtatcaca aaagaaagag aaaaaaaatt ctccctttca gagactttca aatattcatt
    39961 tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc cactcacttg aagatcttaa
    40021 agggaaaaaa gcagctggcg aacccaatac tagctatatg cgtttagcgg aaaaacacgg
    40081 agctaaatta gtcacatatg acaatgccac gaatgatcag tatttaagcg atgtagcaaa
    40141 tggtcgaaca gatgttattc taaatgatta ttatctgcag aaaatggccg tatctgcttt
    40201 accagatatc cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg aaagtggttt
    40261 tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat taaaagagat
    40321 gaaagaagat gggacgacaa agaaaatatc tgaaaaatat tttcaaactg atgtttcagt
    40381 caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc tctaattgat
    40441 ttttccctca tgtggacttc acttcctttc gtactgcaag gcttggtata cacattaggg
    40501 attggcttcg tgtcgtttct tttaggaaat ctggtagggt tgcttttgac agttttaggt
    40561 cttttggatt ggctgcctct gaatgtattt attcgttttt atttatcgtt ctttagaggc
    40621 attcccgcac tagtcctgct gtttctgtta tattttgggt taccttatca attaagtgcc
    40681 ttaacggctt cagtcatctg ctttacgatt accagcagtg catttattgg tgaaatctat
    40741 cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc ttctggtttt
    40801 tctttctttg gggtattgaa atatatcgtt ttgccgcaag cttttcgaat ctcagtaccg
    40861 gcactcagta acgtagctat ggatctagtg aaaggtactt cgttagcagc aacaatcaca
    40921 gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc ggacattcga ttatttttct
    40981 ctctatattt tagttgcact gatttattgg gtcatttgta tcgggatcga aaaagtgcaa
    41041 cagtatttag aaaaacgttc tatgcatcag tcaatctgaa aaatagaaat ggtgctagcg
    41101 tggctggcac catttctatt ttttgtttct tgtatattct gcctagtatt ttaaaaccat
    41161 ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag accccgctaa
    41221 ccatcgaaat agaatcctcg ttataccaat attgcatcga gcaaggcggc ttggttgtag
    41281 aagaagtcac gatgcctgat gaccaaggaa ttgtagatac gcttagctgc ttgacaaaat
    41341 ttgatggaag ccgagaatgg cgatgctatg agctgaaagt gacaaaagca gattttcgct
    41401 ctacagccaa actgtcattt gttggacatt ataattattt tgttttacca agtaaactat
    41461 atgaagaagt aaaaactgaa atccctacag gaataggtgt tttagtgtat cgtccgtaca
    41521 cggttgaaga ggagatgcct gcctctggta catttctgat tgcgaaaaaa gcagcaaaga
    41581 aagagcttgc tgtaccggaa gaagctttga caaaccgctt catggcttct ttattcaggg
    41641 aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata ttttccaaca gatcaattgt
    41701 acaaagaatt gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa cgatattatg
    41761 aacggttttt agaggaaata gctagtcagg caatcaatga gttgcaagaa gaagttatcg
    41821 cgcttcagca tgattatgaa tatttgaggc aacaacagca gatccgtcgg ttgcctacag
    41881 agccattgga gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat ttgacctatt
    41941 agcattaaaa gaagcactgt cgcgaggatt gctctccaat aaaatccaac cagtgatcga
    42001 accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa aaaagcacca
    42061 tcgaatcgct gtgatccaaa atccccaagt aggacaattc aaattattcg atcagcatgt
    42121 gcacagttgg acagtcaaag aaaatagctc tgtcgtccct gcacagatct taacgccaga
    42181 aaatctcagt gaagtactgg attcacctcc agcgtttctt gttttcgatg gacagcacta
    42241 cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat ttttgatacc
    42301 agatactagt cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc gtgactcttt
    42361 tcaaacgaga aagcatgttg aaagttatgc agataaaaca gatgactttt ttagtgatga
    42421 ctatcttttt ttccacgcag acggatatat tggattttct gatttcacga tcgaaggtag
    42481 ccgttatttc gataaagggt tccctagccg agcgcttgct atccatctaa cgtatattga
    42541 tgcttacggc aatatacgcg tgaaacattt tgtctctgac agtaacgact ctgctaaaga
    42601 ccaagcattg aagtttcttg aagcaggaga taaaatgaaa aaatggatca tgcgtcacca
    42661 tcatcaactc ttgattactt caggaatgat tgaattattg gctctttacc agcaacaaaa
    42721 attccctgga ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg aacttattag
    42781 tcacctgcta gatcatccaa aagattggct taaatattat tcaaaagttc aggaactata
    42841 tgaagtgatc caatgattag ataatgtaaa tgaaaaagga atagaaaaga ggaaactgga
    42901 tgtatattcg gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc attaatgccg
    42961 cagcggattt cattgcttcg caaggctctc ctcaatggca aaacgggcag aaaccaacaa
    43021 aagaaacgat tttgaacgat attgaaagaa aagaaagcta tgtattaata gtggaagatc
    43081 aaattgctgg aacagctgca ttagtctcaa gcgtggatcc cgtttacaca aaaatagatg
    43141 gtagttggaa agaatcaact ggtccttatt tatcgattca tcgtgtcgct ttagcagaaa
    43201 gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg attgcagcca
    43261 gcaaaaatat ccaagatatc cgtatcgata cttatccagc taatgaaccg atggaaaaga
    43321 ccatctattc tgctggattc acttatcaag ggatgattga atttcctttt gaacacggcg
    43381 aaagaaaagc atatcaaata atcatcaatt gactataaaa tcatctgagc aaagataaag
    43441 acattaacac tactgaaaaa aacaatcata tgatagccaa gaggttgtaa cagaagtggc
    43501 cagcttcaaa aaacaagaaa gaatttccga aaatgcttct caaatttttg tggaatttta
    43561 gcgtatttta cgaaatagct gctttctgaa actcccttca tcgagaggtc gtgacatagc
    43621 ttttgtcacg acctctcggc ttatcctacg agtattattc gatacattta tatcaataat
    43681 ccttttttta ttttatctgc agcttcttgg aacgctccat cgtttgacac gactaaaagt
    43741 gaggggatag ctttcaaacg tttcaactgt gctaaatctt ctacataaac cgccagtctg
    43801 gctgaaatct gttcagatga atctccacgt tttttcaggc gcgattgcaa tgtttcttta
    43861 gagatcgtaa taaagatagg acggactacc gtcccgaaat actcatataa atgccaaaaa
    43921 ccatttgctg tcaaaacggc gtaacaattt cctactgtca gtttcttttt aatttctgct
    43981 ttactggaac catatttatg ttgagcatat tggtcccact ctgcaaactc atttcccttg
    44041 atcatctgat tgaatgtatt gtcagaaaca aaataataat cctcttgatc tttttcccca
    44101 ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa aatagcttct
    44161 gcgagtgaag tttttccaga accagaagga ccgataaata catagcaata attcattgtc
    44221 atttctcctt tgatgattga acaagtgaaa aagctttgga aaatatttcc aaagctttta
    44281 aaagtaactt tgcttttttg tatattatac cagcaagcca gtcacaatag cacttaaaaa
    44341 gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa gatttccttt
    44401 ttcttcattt aatcccttca tcgcaccaga aatgatcccg atagaagaga agttggcaaa
    44461 tgaaacgagg aagacagata taattgctgt tgtacgctca gtaaaatgga aattaccatt
    44521 tgctaattct gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca tgatactacc
    44581 ggcatccact gcttctttcc atgggattcc gctgatgaag gcaagagggg caaagatgta
    44641 acctaggatc tcttggaaag tgatgccgaa tatcccgtgg aagatagcat tgatcatggc
    44701 aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat ggaaaccatc
    44761 aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt caatgatcag
    44821 ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa tataaccgcc
    44881 aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg ccatataaga
    44941 tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga acaaacgttt
    45001 ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt gtcctaaaat
    45061 agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt tacttaatcc
    45121 tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa taccgataat
    45181 agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt
    45241 tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa
    45301 catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata atacgaaacc
    45361 aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg cttttttgtt
    45421 actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac ctaataaata
    45481 actcaaataa tcactcctat tccaaattcc gttttatttt acctgttttg caaataaaat
    45541 actaatcttt agtgaaattt tcattacttt tttagagtga aacgcattca ttttccatta
    45601 gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa cgtcttagta aagtctataa
    45661 aaaaacgaaa ctatcgatta agctaatagt ttcgttttca acatttataa gtaaactttt
    45721 tctattggta atatagattt tcggaagggt agacaggtgc gcaggtaata tcgattccta
    45781 gttttttcaa tgtttgttca tcatttttat tgaccatcac agtagagtgg gcttgtgctc
    45841 cctcaagttc agctagttta tcataagcaa cttgagcgat tggattcgtt actgcactga
    45901 ttgataaagc gatcaagact tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct
    45961 ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga caataaagga atgtcatctg
    46021 cgatatgggc taaatgtttt actgcattta agataactgc cgctgacgca tccatcaatg
    46081 aacttgtacg cccggtaatg atgacttcat ctggtaattc gattgcaatc acagcagaag
    46141 gatcttttgt aggactttgc gcttgaatct tatctgcata gtcatgggca gggactacca
    46201 cgtttcggtc ttcaggacgt aaaccgactt cttccataat cagtttgatt cgattaactg
    46261 cttcttcgtc gatcaatcct tttttgaagt cgcattctgt ttggaaataa cgacggatga
    46321 tttcttgttt agaggcttct tggacaacgg tatcatccac gatcccaaat cctacacgat
    46381 tcacacccat gtcagtagga gattgataaa cagattcttt accagtaatt ttttcaataa
    46441 tacgtttgat tacaggaaat gtctcaacat cccgattata gttcacagct actttattgt
    46501 aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt tagatcgact gtggctgctt
    46561 cataagcaat gttcagcgga tgtttcaacg gaacattcca tactgggaaa gtttcgaact
    46621 tggagtaacc agctgcatgt cctttttggc tttcatgata cagttggttc aaggaagtag
    46681 cgagctttcc acttcctggg ccaggagctg tcactacaac gattggttta gttgtttcaa
    46741 tatactcatt ttttccaaaa ccatcttctg agacgatttt ttctacgttg actggatagt
    46801 cttcgatttc tgagtgttta tagaccttga tgtttcgtct ttctagttta ttgataaaga
    46861 ctttcgtggc aggctgtcca ctatagcgag tgataacgac actgttgatt aataagccat
    46921 aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc ataagtaatc ccataatcac
    46981 cacggatctt attacgttca atatctccag catagacgca aatcagtatt tcagcttgat
    47041 ctttcaattt ttgcagcaat ttgattttcg catcttcttc aaatcctgga agtacacgtt
    47101 tcgcatgctt gtcgtcaact agcttcccac caaattctaa atataatttg tcatagtggt
    47161 tcactcgttc caaaatatac gctgactgct cttcaatata tttttctgaa tcaaaaccaa
    47221 tttttttcac taggttccct ccttaagtac taaagcattt tttcattttt ttgaggattc
    47281 gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc
    47341 tagatagtta ttcattggta tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga
    47401 cagaaaaaga aaaacgcata taaacactat atccgttcga tttttaccgg ttatgaaaga
    47461 atgcttgaag atcccgaatt ggaacaattg acgtttactt atctaaacga agaaacgcag
    47521 ctaactcgtg atgagcatca gcgcattcat ttcacaacaa gagatctgcc tagtaaataa
    47581 aaaataagat aaaggtcatc ggattcgatt ctgtcctatc agatggaaag actagttgat
    47641 cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt atggagcatt gcccgctctt
    47701 ttttataaca attttttaca gaaaatggta ctattgaagt attaataagt gaaaaacaga
    47761 tgagtaagag gaggcaagta gtatgaaaga gccagataca aatgcaaatt atgagccaga
    47821 cgaactagag gagacgaatg cggatcagga tacaggacaa gaatcgtctt ctttttacga
    47881 tatcaaagaa aaagcaagca tgaatctttc ccaattatta gaagacttac aggcttttga
    47941 aaaagctgtc cgcgaagaag atatccctga gatctaccgt ttatacaatg gccgattgaa
    48001 tgaagaatta aaaagaagct caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa
    48061 gatccatgat cgttttttac aggaatttcc ttttatggaa caagtagaaa acatctcgcc
    48121 aacgatgagc tattacaaaa tcggcactta ttaccatgat cggcccacgg tcggtattga
    48181 tgcaagtctg ccagaaattt ttgttttgcc taagatcgat gaagagtggg agaaatattc
    48241 ccaaccggag tcaacggact atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac
    48301 tgctcagaca gaaattgaac gtttagatga acaaataaaa gaaatcaatc gacaaatgac
    48361 tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga
    48421 attagagaag aagaagcaag tactatctaa tgaaaaatta ggctggcttc cttatattga
    48481 gacgcctgaa acaatccagc agcaaaaaga agcattgaag caagaagcaa gagcagacca
    48541 gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa
    48601 agagggattc ggtcaagcaa tccatgaatt cttgatgaat tatcttggca atgagaatcc
    48661 taataatcaa tctaatgaag gaggttccga gtatgaatga acacgatgaa gcagaatgga
    48721 caaaaaatga agaacaaaca gaagatcatg aagaaacgaa cacttcattg gaagaacaaa
    48781 caagtaatcc tctggatgac acagaagaaa tccctatgct agaggaaaca aaaatacacg
    48841 aaaaagaaaa agtaactccg acgaatacga atactgcaga aaacacccat gcttacgaaa
    48901 taactgaaaa tcccgggtat ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt
    48961 tgattgaaac agcggaagca gaattagcgg atttacgtat gagaaaagat ttgatcgaat
    49021 cagattttcc acaattatta gaatcatatg atcgttttat attgaatgaa gaaccaatct
    49081 ccacgatcgg aaagacaagc ctgatcgaat acttcacgta tgaattactg aaaccgttga
    49141 atgatattgg tctggaacgg tcaaattcag cttatgacac atggaaaaca agacattttt
    49201 cgatcaatta tacgttgaat ggacaaaacg aattagtctt ttcctttgta ttaccgacca
    49261 tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa cgtatcacca gagacaatgg
    49321 aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg acactggtca gtggatcgag
    49381 tattctctgc agggcagatc acgatattca atcacaaatt gaatcaatta ttagcccatg
    49441 caagaacact tggttttacc gttaatcaga cattactaga caatacgaag ccacttcgtt
    49501 tgaatcttca gagcgaattt gaattgacga atgaagtatt ggatgatatt tttatcgtgg
    49561 caatgaaaaa tccatcctac gatatggaaa agatcaacga aactacttat caagtacgat
    49621 tagataaagg acagtcttta acgataacaa caaacgaaaa agatcaaaca gtcttgtcga
    49681 tctcatctgg agactaccca cgatctgtca ttgatttctt tatcaattac gaattcttgg
    49741 tcccattaat ggtgagaaag tcctgattgg ataagttgga ttaccagatg tcattcagat
    49801 aattctgttc caaaggactt gcggcagtta tccagaataa ttgtcacaag tcttttctgt
    49861 atgtactaaa tggacgtgaa taacttgctg atgaaggagg gatttcatta ggaatgtcca
    49921 caaataaaat agcacagctt agcttattgt ctgctgcttg tgtagcaggt aggatcgctt
    49981 ttcaatttat tccgaatttc cagccaatga ctgctatttt tttatttata gtactttact
    50041 taagcttaaa agatgcgtta attgtcatga gtctttctat tgcgatcagc agtttttatt
    50101 ttggtgttgg accatgggtc atcggccaat ggatcagcta tactgttgtt ctgagtttgt
    50161 tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct atggttaaag ggagtctgtt
    50221 tttttctggc gggcatggtt tacggaatag gtatgaccgt ttttgatacg ctgttatatc
    50281 gtttgcctca accttggatc tattaccttc aaggggtgtc gtttgatttg atgcacagta
    50341 taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt caaacgtttt tacaatcatt
    50401 caggaggaaa aaatgaaaaa aataatttat agtttgcttg caatgagtgt cagtatactc
    50461 attttgacaa gttgcagtac aacctataca caatcggctt ccaaccaaaa ggaggaagta
    50521 aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa
    50581 gaagtgttct tcaaaaaagg cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc
    50641 aaagaagata acggcttcat cacttcgctt gaagggcact cacaggatga gaaaaatcaa
    50701 ctctattgga tgtttactgt ggacgggaaa agtgctacta ctggtgccaa agagatcaaa
    50761 ttgaatgatg gacaatcggt cattttcaat ttgtctaaac tataagtttc ataaaaaact
    50821 gttaaagggc gaatattgcc ataattcagt gagttcagtt gatgaaagaa ggaaaataac
    50881 atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg ctgctctagg ggctaatatt
    50941 ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg gcagtgccgc catgctaaac
    51001 gaaagcatcc acagtatcgt tgattgcagc aatcagattt tgctgttatt cggtgataaa
    51061 caagccaata aaggacagag cgagttgcat caatttggtg aaggacgagc aaaatacttt
    51121 ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa
    51181 gcttttgaaa agttgacgca tccatcccat gaagtgggta atccgatcat tgttatagca
    51241 atactgttat ttggtttggt aatcgaagga agttcgttac gcgtggcaat gaaagaaatc
    51301 aaagaattga atactgaaaa actttccacg atgcgctttt tgcgcgaaag tcgacacagt
    51361 gaaatactga ttatttttac ggaagacttg tgtgctgtaa tcggtttggt cttggcttta
    51421 gcaggaacaa tattgactta tgcaaccggg attgctgcat ttgatgcgat cagcggattg
    51481 ctgatcggtt tcttgctgat gggagccgcc attttcctag caaaagaatt ttatagtctg
    51541 atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt
    51601 cctgagatca gtcggctgat cgatataaaa acagttcatt taagtccaac ggagatttta
    51661 gttgcagcaa aagcagatat cgttggtaac aaagagaatg aagcttactc ggtcatcaat
    51721 gatattgaaa aaaatatgcg tcagtctctt cctgacaaga agatgtatat atacattgaa
    51781 acagataaat atgacccaaa ttattctcgt aaataaaacg tttattcatg aattgaagca
    51841 aaaatgagaa aaatataatc ttgtttttaa ataaaacggc tacaatttag cgaatttatg
    51901 ttacaatgag tccgctgttt atttgttgta attaaaggag atattttaat gaacaaaaag
    51961 aaaagtgcgc catggcttca agctttgcat gcagtgatgc cactttatat aagctatata
    52021 ccagtaggat tagcttgcgg agtattgttg caaaaagttg gatttgatcc tctcttatca
    52081 ggactgttat cgattctggt cttttccggt ggcgcgcagt ttttagttgc atctatgctt
    52141 acgacgcaag catcatttgc aacgacatta ttgatggttt tctttttaga attacgctat
    52201 acattattag gagcaagtct tgccggtttt atgaaaaaag aaaagcggac gtttttggct
    52261 gtcttttctc aatcactgaa tgatgaaaac tatgcggtga actacttaaa gttttcaact
    52321 gacccttcct ggaataaaag aaaggcttta tatgtcaatt ggttctcaat gactgcatgg
    52381 gccggtagca atatgatcgg taattttttc ggctcggtga ttcgagtaga tgctgacctt
    52441 gtccattttg ctttgacggc catgttcatt ttcatgttta tcatgcagat gaaaagcgca
    52501 ctactgatct ttacaggttt attctcaggc gtattagggg tcgtattcat ggtattattc
    52561 caaaatactt tcggattgat tgcagcaaca gtcattgctt catttgccgg atttatgctg
    52621 gagaaggcat tcaaaaaaga aaaagctaag aaactaagag accgcggtaa agatgtcaat
    52681 gagccgctct tcaacttccc ggaacaggag gccaatcacc atgactaaac tatatttgct
    52741 acttttagtt gcttgtctgt tcatcgtggc atatatcccg cgcttgttcc cgatgcttta
    52801 ttttactcat cgaaaagtgc catcctggtt cagtgactgg atgaaatatg taccggtggc
    52861 attatttgcg gcgttagctt ttaaagatgt tttcattact catgaacatc tggatattgc
    52921 ttggaatatc aaaatagctg caatgattct agtagcaggc gtggcttaca aaacacgttc
    52981 gatggcgttg tcagttttga caggacttgc gtctgtcttt ttgctgtcta tgctttaaac
    53041 tgttttattt tattaaaaac agagattgcg acataagttg ttcaaactga tctatccgaa
    53101 ctgcatttgc aacagtcaat tctggatgca agtttattcg tagggataaa aaagattagt
    53161 gttggcttat gatgcaagct cttttttatt ttaggaatta gagagggatt tgtccttcta
    53221 tgattttttg ctataatagg gaagatgttt attttatgaa agaatagttg agggaattta
    53281 aatgaataaa aaattgatgc aaggaacatt ttggctaacc tttgctaatc ttctatgtaa
    53341 agttttaggg gtagtctatt tgattccctg gctaagtatg atggggaaca atcaagatgg
    53401 tatgcttgct acgacattgt ataatgttgg ctatcttcct tatggattgt tcctgatgct
    53461 tggtacagtc ggatttccaa atgcgattgc aaaaaaagta gctgtagcca caaaagaagg
    53521 agataacgct gcttgtcggc tgatttttag aagtacgatc aatatcatgt ttgtcatcgg
    53581 tatcgcctct gctgcactga tgtatttatt tgctccttta ctagccggaa tcagtccgat
    53641 tgctaacgtt aataatggga tactagctat cagaagttta tgcccatctt tgattgctat
    53701 cccaattctt agtgcaatga gaggatattt ccaaggaaaa aatgatctgc ggccttatgg
    53761 aacttctttg attatcgaac aggctgtgcg agtgatcgtt atcttggctg gtacttatta
    53821 cttgcgcgta ttgactgacg gaaccatatt agaagctgta ttgatcagta cagtcgcttc
    53881 tttcttcggt gggttagctg cgattatcca tatgtatatc gtcggacgca gaaaagactt
    53941 cttcgaactt aaagattttt tgatttccag ccgttatttc gaaagagaaa accgatctgc
    54001 ctctgtctct atcattcggg aaacactacc gtttattttt gtcggctcag tcattactat
    54061 cgttcaaatg atcgaccaag taacgatgaa accactgctt cactttttcc gtccagagat
    54121 cgcagatcag caactggaat tcttattcag ccgtgcttct gtcaatccaa ataaattgac
    54181 attgatcctg atctccatgg taggaacagt agctataagc agcttgccaa tattaagcac
    54241 gatgaagaaa aaagatcgga tacaaatcga aaaaacagta ggagatagtt tttcgattgc
    54301 gttattgatt cttcttcctt cattgactgg aatgtcttta ttagctggac cactatacac
    54361 attgtttttt ggctatgatc ctgaaagtgt cggatatttc caaatggctt tgctagcttc
    54421 tttgtttttc tctttattca cgatcctatc tacgatgctt caatctttga atcaccatcg
    54481 ttttgcgatc cgattaactg tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg
    54541 attaggatta gttggcggat acgggatgag cttatcaaat ggcttagcat tcggtattgt
    54601 ctttgttcgc ggctatcact ttatgtgcaa agaataccga atagcacctt tagcaaagat
    54661 cagcaatttt ttcttaaaaa ctttccgctc caccctgatt atgcttgcag tatgttttgc
    54721 cgttttcttt ttgctgaatc agcgattgtc aatggaatca aaaagctatg caatgattta
    54781 ttgtgtggct gtaggaagta ttggcggctt agtattttta ttttctcaat tcggaaaaaa
    54841 tgggtttcga atgttgaaaa atgttaaaca tcatagcaga agcaaagaat aggttaacta
    54901 atcgtttttt gctgtcactt gaaaaacggc aactcgtatg attacgcttg tcgtttttct
    54961 attttccaat tttagtttca tatctaatct tacttatgat atagttataa ggtctggaaa
    55021 aattctttga aacgaggaaa ttaaacacat gaaacttgca gtagttacag atagttcggc
    55081 atatctgccg gaacgtatca agaaccatcc cgacttattc gttattccaa tccctttgat
    55141 tatggatggg aaaatttata acgaaggaat cgatatcgaa gcggatgaat attatggact
    55201 tttaaaaaac agtaaagatt ttcctactac ttcacaacct gtcgtagggg aagttttggg
    55261 tttatatgag gaattgaaag agaaagggta tgacacgatt ttaagtatcc atctttcttc
    55321 agggatttct ggctttgtca acacattatt tgcgatgaaa gatgacatca gcggagtaac
    55381 ggtcattcct tatgactcta agatcactag tatgccgatg ggacacatgg tagaagctgc
    55441 cttagactta aatgatgaag gtcagtccct tgatgaaata cttgcacata tcgatcgaat
    55501 ccgagataat acctacgctt atctgattgt agatgatttg aacaatttag tacgaggagg
    55561 acgtttgaca aatggtgcag cgctgattgg cagcttatta aaaatcaaac cgatcctgac
    55621 attttctgaa gggaaaattg ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc
    55681 tcgagctgaa gaaattattg gtagaagaaa taacgaaata caaccgccag tcaaacttta
    55741 cgtgatacat gcaaataatt tagaagtagc gattgcggag aaagagaaat tgcagaaaaa
    55801 atatccaaat gcagaaatcg aaattggtca tttcggacca gtcattggta cacatttagg
    55861 tgaaaaagcc atcggcattg ccatttcagc gcaataattc cttttattca aaaaacatac
    55921 caaaaatatt ccaacatcat cttggacttt ctacatgtta ctaatgagcg tgtaaaagaa
    55981 cgagatggtg tttttatttc tatcaatgaa gctctttttc tttgcaatcc tcacttcaat
    56041 cctctataat gtcgcatata gtttagtaaa tgagtaaact actaatttac ttatttacta
    56101 aaaataaaag gatgaggagt taaaataatg gaaataccaa caaatttaaa acataaacca
    56161 gtaatcgttg tagaagacta tgataaagtt gatggaagaa atgcattaaa tacggatgca
    56221 aaagggcttt cactgggcct tgctcagtgg aatgatcgtg gaaaagtcga tatctcaggc
    56281 aaggtatggc gacatactgg agaaaaatgg tcacgacagt cggaagaatt accaatcaca
    56341 cgtattttag atttagcgat cttagctgca caaggaagtt tatacttcca agatgcttat
    56401 cgttatgaaa aattttatga tccagaaaat ccagtcgtag acatcattgg attacaaggg
    56461 aaccgaatga cggtagaagt agataccaaa aatccaaaga tcgatgaaga catcattttg
    56521 tattacgata cactacaaaa agacggagaa ctgattgggg aacgattccg tattttaaaa
    56581 cgtcttttag atgagctagg atattaatag acagagatga tcaaaagggg aagagaagca
    56641 tggagaattt ctcactaaca gaaaaccgaa ccttttcacg gctagaaaag caaatgatct
    56701 ggaagaaacc gaaaagccat cggacaagtg cagaagaatg gcggatcgcc tccgaaatca
    56761 aagggaattg gcaagatcct gaaatgaaaa tcagtaacgt acttttggaa ggagatgccg
    56821 ggtcggggaa gacacaatta gccaaagcac tatctgctga tctgcaactt ccctatacaa
    56881 aagtgacctg ttttgcagat atggacaaat cagatgtttt cggagcctta ttacctgtag
    56941 tagattctaa taaggaacaa gaccaagaat tactagaagc tatttaccaa acagatacac
    57001 ttgaagctgt actgacactg atccagaacc attacggcat tgatcctttt tctgctaaag
    57061 aaaaattagc agagctagtt caaaggatcg aagctgatta tccagatgca atccattaca
    57121 gctattaccc ttctgaaatc gttcgagcga ttgaaaaagg atatttatta gagattcaag
    57181 agccgactgt catccgagat gcctctgttc ttgtggcact taactcagcg ctcgaaccaa
    57241 atgggatgtt gaatttaccg acaggaatca ttcgtcgcca tccagattgc gtcattgtga
    57301 tcactacaaa tcgaaattac caaggaaatc gcccattaaa tgaatcttta agagatcgga
    57361 tgcaacatgc ggaaaaaatg gatcttcctg ccttagaagt catggcagaa cgtgcgatgg
    57421 cgaaaactat gatccaaaaa caagaacttc tcttaaagat ggcagagatt atccgtttgt
    57481 tggatgaaac tgcaaaagct aacgccatca aaggtgtagc aggaatgcgt tcttattttt
    57541 attgggcaaa tacctataag caaggacaag atttactcca gtcgatttat ccaaaagttt
    57601 tgtataaatt atccactgat tcagatgaat tggcaattct tgaacaagct ttgtctgaca
    57661 gtggattgct actggaactt caggacatat tgagaaaaga aaaatggggt catcctcaat
    57721 ctgagcatac aaaaggaaga accattagcg ccgaagaagc aaatgaacga aacatcactg
    57781 aaaatgctga agaaattctg cgcttagatg aaaagaaatc gttgtctgct gaagaaccaa
    57841 atgagggaga agagaaacaa gaagaaacaa tagaaaaaaa cgagattcct cctcaaatgc
    57901 aaaaagaacg cagtagcgat atcgaaggaa aaaatcaaaa agaacaacaa acaggagatg
    57961 attctcaaga aatgtctgct tctgacatgg aagcacatga taaggaaatc aagaagcaac
    58021 tgaataaaga agcgcgacaa atcatgaagg aaacgattca tgaaaaagaa ggtctcatcg
    58081 ttcaccgacc agctttccaa ggaaaaacgc cggaagcacg tgagctacac atgcaagtaa
    58141 tgccaatagt agaatcactt agccggcaaa ttttagagtt actggataat gaacaaacag
    58201 agacgtacca aaaaggaaaa tacgaaggtc agcggttcaa tgcaagcaaa gtagcatatg
    58261 gtgatttgag aaattttgac aaaaagaacc cgccacatga gcaaccttct ttagcggtag
    58321 ctgtacggat cgatgaatct gggtcaatga tacgggatga tcggatcgaa gcggcaaaaa
    58381 aagcagcgat tgccattgcc gaattttcta agaaagtcga tatcccattg ttgatttacg
    58441 gagatacagc agaccgttcg tcaagagaaa agacttcttt gttttcttat aaagaatttg
    58501 aagacggatt tcaatggtta aatgaaaaat tagtcactat gaaaccaagg caaaacaatc
    58561 gtgatggtgc tgcacttcgt ttagcagcag aaaaattaaa cagacaatcc gcaacaacca
    58621 aactgttgct taatattagt gacggtcagc caaaagcgtt gccggattat acaggcaaaa
    58681 aagcaaaaga agatatccag tctgttttga aagaatatga acgtcaagga atcctatttg
    58741 tctccgcagc tattggccaa gacaaagaag aaatcaagtc gatctacggt gaaagtcgtt
    58801 ttgtagatat tactgacttg aatgaatttc ccaagcaaat gattcaactg attgcccgct
    58861 atctatgata aaa
//
LOCUS       DAZKCC010000008        56692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000008 DAZKCC010000000
VERSION     DAZKCC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.008"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..533
                     /locus_tag="ACG8VH_000501"
     CDS             15..533
                     /locus_tag="ACG8VH_000501"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9109087.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            661..1344
                     /locus_tag="ACG8VH_000502"
     CDS             661..1344
                     /locus_tag="ACG8VH_000502"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP9109088.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1653..1726
                     /locus_tag="ACG8VH_000503"
     tRNA            1653..1726
                     /locus_tag="ACG8VH_000503"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Arg,seq:tct)
     gene            1885..2244
                     /locus_tag="ACG8VH_000504"
     CDS             1885..2244
                     /locus_tag="ACG8VH_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP9109089.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2367..3998
                     /locus_tag="ACG8VH_000505"
     CDS             2367..3998
                     /locus_tag="ACG8VH_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP9109090.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4060..4701)
                     /locus_tag="ACG8VH_000506"
     CDS             complement(4060..4701)
                     /locus_tag="ACG8VH_000506"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9109091.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4887..4963
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4983..5291
                     /gene="rplU"
                     /locus_tag="ACG8VH_000507"
     CDS             4983..5291
                     /gene="rplU"
                     /locus_tag="ACG8VH_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9109092.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5318..5662
                     /locus_tag="ACG8VH_000508"
     CDS             5318..5662
                     /locus_tag="ACG8VH_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP9109093.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5675..5968
                     /gene="rpmA"
                     /locus_tag="ACG8VH_000509"
     CDS             5675..5968
                     /gene="rpmA"
                     /locus_tag="ACG8VH_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9109094.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6402..9209
                     /locus_tag="ACG8VH_000510"
     CDS             6402..9209
                     /locus_tag="ACG8VH_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9109095.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9400..9891
                     /locus_tag="ACG8VH_000511"
     CDS             9400..9891
                     /locus_tag="ACG8VH_000511"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9109096.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9994..10983
                     /locus_tag="ACG8VH_000512"
     CDS             9994..10983
                     /locus_tag="ACG8VH_000512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9109097.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11016..11819
                     /locus_tag="ACG8VH_000513"
     CDS             11016..11819
                     /locus_tag="ACG8VH_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9109098.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11840..12754
                     /locus_tag="ACG8VH_000514"
     CDS             11840..12754
                     /locus_tag="ACG8VH_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9109099.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12837..13211
                     /locus_tag="ACG8VH_000515"
     CDS             12837..13211
                     /locus_tag="ACG8VH_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP9109100.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13350..13595
                     /locus_tag="ACG8VH_000516"
     CDS             13350..13595
                     /locus_tag="ACG8VH_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9109101.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13767..14831
                     /locus_tag="ACG8VH_000517"
     CDS             13767..14831
                     /locus_tag="ACG8VH_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP9109102.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15077..15502
                     /locus_tag="ACG8VH_000518"
     CDS             15077..15502
                     /locus_tag="ACG8VH_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9109103.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15495..15944
                     /gene="nusB"
                     /locus_tag="ACG8VH_000519"
     CDS             15495..15944
                     /gene="nusB"
                     /locus_tag="ACG8VH_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9109104.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16041..16895
                     /locus_tag="ACG8VH_000520"
     CDS             16041..16895
                     /locus_tag="ACG8VH_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP9109105.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16896..18233
                     /gene="xseA"
                     /locus_tag="ACG8VH_000521"
     CDS             16896..18233
                     /gene="xseA"
                     /locus_tag="ACG8VH_000521"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9109106.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18238..18462
                     /locus_tag="ACG8VH_000522"
     CDS             18238..18462
                     /locus_tag="ACG8VH_000522"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9109107.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18464..19354
                     /locus_tag="ACG8VH_000523"
     CDS             18464..19354
                     /locus_tag="ACG8VH_000523"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9109108.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19363..20181
                     /locus_tag="ACG8VH_000524"
     CDS             19363..20181
                     /locus_tag="ACG8VH_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP9109109.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20288..20737
                     /locus_tag="ACG8VH_000525"
     CDS             20288..20737
                     /locus_tag="ACG8VH_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9109110.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20755..22437
                     /gene="recN"
                     /locus_tag="ACG8VH_000526"
     CDS             20755..22437
                     /gene="recN"
                     /locus_tag="ACG8VH_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9109111.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22492..22608)
                     /locus_tag="ACG8VH_000527"
     CDS             complement(22492..22608)
                     /locus_tag="ACG8VH_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP9109112.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22681..23637)
                     /locus_tag="ACG8VH_000528"
     CDS             complement(22681..23637)
                     /locus_tag="ACG8VH_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9109113.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23808..24176
                     /locus_tag="ACG8VH_000529"
     CDS             23808..24176
                     /locus_tag="ACG8VH_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP9109114.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24346..24777
                     /gene="mraZ"
                     /locus_tag="ACG8VH_000530"
     CDS             24346..24777
                     /gene="mraZ"
                     /locus_tag="ACG8VH_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9109115.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24798..25757
                     /gene="rsmH"
                     /locus_tag="ACG8VH_000531"
     CDS             24798..25757
                     /gene="rsmH"
                     /locus_tag="ACG8VH_000531"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9109116.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25762..26163
                     /gene="ftsL"
                     /locus_tag="ACG8VH_000532"
     CDS             25762..26163
                     /gene="ftsL"
                     /locus_tag="ACG8VH_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9109117.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26164..28356
                     /locus_tag="ACG8VH_000533"
     CDS             26164..28356
                     /locus_tag="ACG8VH_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9109118.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28382..29344
                     /gene="mraY"
                     /locus_tag="ACG8VH_000534"
     CDS             28382..29344
                     /gene="mraY"
                     /locus_tag="ACG8VH_000534"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9109119.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29358..30719
                     /gene="murD"
                     /locus_tag="ACG8VH_000535"
     CDS             29358..30719
                     /gene="murD"
                     /locus_tag="ACG8VH_000535"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9109120.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30745..31833
                     /gene="murG"
                     /locus_tag="ACG8VH_000536"
     CDS             30745..31833
                     /gene="murG"
                     /locus_tag="ACG8VH_000536"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9109121.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31848..33068
                     /locus_tag="ACG8VH_000537"
     CDS             31848..33068
                     /locus_tag="ACG8VH_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP9109122.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33224..34549
                     /gene="ftsA"
                     /locus_tag="ACG8VH_000538"
     CDS             33224..34549
                     /gene="ftsA"
                     /locus_tag="ACG8VH_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9109123.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34572..35813
                     /gene="ftsZ"
                     /locus_tag="ACG8VH_000539"
     CDS             34572..35813
                     /gene="ftsZ"
                     /locus_tag="ACG8VH_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9109124.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35830..36507
                     /locus_tag="ACG8VH_000540"
     CDS             35830..36507
                     /locus_tag="ACG8VH_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP9109125.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36521..37120
                     /locus_tag="ACG8VH_000541"
     CDS             36521..37120
                     /locus_tag="ACG8VH_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP9109126.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37201..37422
                     /locus_tag="ACG8VH_000542"
     CDS             37201..37422
                     /locus_tag="ACG8VH_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP9109127.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37471..38253
                     /locus_tag="ACG8VH_000543"
     CDS             37471..38253
                     /locus_tag="ACG8VH_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP9109128.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38274..38981
                     /locus_tag="ACG8VH_000544"
     CDS             38274..38981
                     /locus_tag="ACG8VH_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP9109129.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39243..42041
                     /gene="ileS"
                     /locus_tag="ACG8VH_000545"
     CDS             39243..42041
                     /gene="ileS"
                     /locus_tag="ACG8VH_000545"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9109130.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42090..43616)
                     /gene="zwf"
                     /locus_tag="ACG8VH_000546"
     CDS             complement(42090..43616)
                     /gene="zwf"
                     /locus_tag="ACG8VH_000546"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9109131.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43631..44278)
                     /locus_tag="ACG8VH_000547"
     CDS             complement(43631..44278)
                     /locus_tag="ACG8VH_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9109132.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44462..44791)
                     /locus_tag="ACG8VH_000548"
     CDS             complement(44462..44791)
                     /locus_tag="ACG8VH_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP9109133.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44968..45696
                     /gene="sfsA"
                     /locus_tag="ACG8VH_000549"
     CDS             44968..45696
                     /gene="sfsA"
                     /locus_tag="ACG8VH_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9109134.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45712..46725
                     /locus_tag="ACG8VH_000550"
     CDS             45712..46725
                     /locus_tag="ACG8VH_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9109135.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46725..48002
                     /locus_tag="ACG8VH_000551"
     CDS             46725..48002
                     /locus_tag="ACG8VH_000551"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9109136.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48065..50767
                     /locus_tag="ACG8VH_000552"
     CDS             48065..50767
                     /locus_tag="ACG8VH_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9109137.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50919..51236
                     /locus_tag="ACG8VH_000553"
     CDS             50919..51236
                     /locus_tag="ACG8VH_000553"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9109138.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51266..51586
                     /locus_tag="ACG8VH_000554"
     CDS             51266..51586
                     /locus_tag="ACG8VH_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9109139.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51694..53154
                     /locus_tag="ACG8VH_000555"
     CDS             51694..53154
                     /locus_tag="ACG8VH_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9109140.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53222..53443
                     /locus_tag="ACG8VH_000556"
     CDS             53222..53443
                     /locus_tag="ACG8VH_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109141.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53474..53656
                     /locus_tag="ACG8VH_000557"
     CDS             53474..53656
                     /locus_tag="ACG8VH_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP9109142.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53656..54069
                     /locus_tag="ACG8VH_000558"
     CDS             53656..54069
                     /locus_tag="ACG8VH_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP9109143.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54192..55373
                     /locus_tag="ACG8VH_000559"
     CDS             54192..55373
                     /locus_tag="ACG8VH_000559"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109144.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55444..55608)
                     /locus_tag="ACG8VH_000560"
     CDS             complement(55444..55608)
                     /locus_tag="ACG8VH_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109145.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55762..55835
                     /locus_tag="ACG8VH_000561"
     tRNA            55762..55835
                     /locus_tag="ACG8VH_000561"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55796..55798,aa:Arg,seq:cct)
     gene            complement(55904..>56692)
                     /locus_tag="ACG8VH_000562"
     CDS             complement(55904..>56692)
                     /locus_tag="ACG8VH_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9109146.1"
                     /translation="AKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKDS
                     LKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDIDF
                     EDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIKN
                     PHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILS
                     VSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        18706 a         9576 c        12176 g        16234 t
ORIGIN      
        1 agaagttgaa aacgatacct gattacaaaa aaataacttt tataagttct ataacgttga
       61 ttatattttt aattcttatt cttctcgtcg tcttggttcc ccgaattttt ggtttgactc
      121 cttatattat tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg
      181 tacgaccgaa aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt
      241 caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt
      301 atacaaaagc tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta
      361 tcggtagtcc catctttcag attccatata ttggactagt cctttcaaaa acggcgtttg
      421 catggattca ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga
      481 atacctttgt agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt
      541 aaaaacataa atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac
      601 actcatgcag cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt
      661 atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt
      721 acccagcaaa aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca
      781 gaagtaccag ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt
      841 cagcctgaac gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc
      901 acgacaatcg gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag
      961 aaagctaaaa aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt
     1021 caagcagcag atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt
     1081 gcaaaatcaa aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc
     1141 gttttgatgg tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt
     1201 ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat
     1261 catcccgatt tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag
     1321 gaatcagaaa caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa
     1381 tcgagggctc aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat
     1441 acaggaaatc taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga
     1501 taaccttaga acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc
     1561 caaaataatt cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat
     1621 tatctataat tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat
     1681 ccgccttcta agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta
     1741 gaaactgagg tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa
     1801 ggcaaataaa ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa
     1861 ctcatcagaa aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt
     1921 gcaattgaat ttttttatat catgtatatc gaaacggtga gaactgattc tgacacgaca
     1981 agtagggttt ttaaaatgag taaagaagag ttaagcagaa aatctgttca aacattgttt
     2041 aagaaccaag gagtgtacaa tggactatta ggagtgggac tactttatgg agcttatctt
     2101 tcttccgcat ctaaagaaat cacaagtatg ctattaatat ctattttttt tgttgcatta
     2161 tatggcagtt tgactagcga tcgttggatt ttactcaaac aaggtgggct agctctgttg
     2221 gcattgcttt ctattttctt ataaatatag aaaaaataag aatttatcta acaggaaact
     2281 ttgtgaaaaa tattacttta tctcatgaaa tatgatattc taatgaatgg tattttagaa
     2341 aatttcatga aaaaggagtt agataggtgt tcaaaaatta tgtttcctca ctaaaaaaag
     2401 aattttcagg ttatgatctt tcaagatttc agaaagatct tttggctggg atcacagtag
     2461 ctgctgtagc attgccttta gccttggctt ttggggtatc aagcggagct gatgcggccg
     2521 caggtttgat tacggctatc attgccggta tcgttattgg cggtttatca ggcggttttt
     2581 atcaaatatc aggtccgaca ggtgcgatgg cagctatctt gatgtctatt gcggcaacaa
     2641 agggaatgca aggtgtattg cttgctactt ttttagcggg tctattttta cttgcagcag
     2701 gacttttccg attaggaaca ctgacttctt ttatcccagc acccgtaatt acggggttta
     2761 cttcaggtat tgctatcatt attgcattag gtcagatcga taatttattt ggtgttcact
     2821 cggaaggtac aaacgttatt gaaaaactaa gtagttatca aacgatagga tttccgataa
     2881 acacagcctc tttgatcatg ggagggctag tcattttagg gatgtttatc tatccaaaaa
     2941 aatgggcgaa acgaatgcct agttcgctgc tggctatcat tttggcaaca gcagttatgc
     3001 tattgttcca tttaccagta gcaactgtgg gtaagattcc acaaacattg atcagtagta
     3061 atcgtttgaa tatgggagat tttagctttt ctgccttgca acaagtggca gttccagcta
     3121 ttagcattgc tttattagga atgattgaaa gcttgctatg tggtgcttcg gctggccgaa
     3181 tggcaaataa gcccttagat agcaatcaag aattaatggc gcaaggaatt ggaaatctgt
     3241 tgcttccttt ctttggcggg attcctgcta ctgcggccat cgcccgtaca agcgtggcca
     3301 tcaaatctgg tgcacaaact agattagctg gtatgataca tgcagttgtc ttactgctct
     3361 ccatgcttgt ttttgcgcca gtgatgtcac agattccaat gccagcactt gcaggtgttt
     3421 tgattgtgac ggcttggcgt atgaatgaat gggaaacgat caaggagcta tttactaata
     3481 aatattggtc agcagttcta ttgtttatcc ttacgatgat ttgtacagtt atctttgatt
     3541 taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt gtcaaaagcg
     3601 ctgctatcac gatttcatta gaaacgatcg attggcaaag aatggggctg ccagaaacgg
     3661 aaaagctgaa taactggaca gtagtgtata ttagtggtcc actgtttttt atgtcttcag
     3721 aaaaactcaa aaataaattg gaagaactaa atcatcacga tggtgtcatt ttctccatgc
     3781 gaggcgtacc aagtattgat ttgacggcac gtgcagtttt tgatgaattt caagaaaaag
     3841 cagagcaaag aggacaaacg attatctata cttccttgca gccagaagta gaaaaacaac
     3901 tttctcactt gtgggaacat acattcacgc atcaacatca aacagttgct catgcgttga
     3961 cagctttaca tgcgaaatat actgcaatta gccattaaaa caatgaaaaa gaacagacaa
     4021 gactgtaaac tgaagatttt atggtcttgt ctgttctttt taatattgct gtaaaagtga
     4081 agctagtact tgccagtctt gatggataac aggtacaagg gagcgattgt agaaaatagc
     4141 tgctggatga aaaagtggaa gaattcgata gtttttttct gaccattcgt aggcatcctt
     4201 ttgttctgtt aattgtaaga caggccctgt atagagttga ccatgatttt tggtaaccgt
     4261 atattctttg ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa ttaattttgg
     4321 ctggaccttc atcagttcgt aatccaatat tggtgcatgc gcaaatactt cggaacgagt
     4381 aggtgtccga tttggataaa cagtttctga ctgttgtgtc cgtttattga tccgatgagt
     4441 cacgcgatac gggcggctcc ttacagcact ggtgatatac acctcttctc gtgttaagcc
     4501 agttgaagaa agtgcctgca tcaattcttt tccagcttga ccgctaaaag gaacatgcgt
     4561 atcaatctct gttttccctg gggcttctcc cacgagcatc agtttaggat gaagaggtcc
     4621 ttgcccagcg ataaatcctt ctacaggacg gccgttcatt cttaaagaag cttcttgtat
     4681 cagttctttt gggtattcca tttatgctcc tcctttttct tttattataa gaataaaaga
     4741 aacacaatgc gactatttta tttggtagaa ctgtgaggat agaaaagcag aagtattgct
     4801 tgaatttttg ttttactttt tgattttaaa tttagttgac taacggttgc tgttctggta
     4861 agctagtact gttgtaattg tggactccac agctacaacc gctcagaaca agtaataagc
     4921 acagcatatg ctgtcacttc ggatggcgag tctaagtata tcagaaggag gtgctttata
     4981 gcatgtacgc aattattaaa actggtggta aacaagtaaa agttgaagtg ggacaagcaa
     5041 tctatgttga aaaacttgac gttgaagcag gagaaaaagt tgtttttgac gaagttatct
     5101 tagtaggcgg agaatctaca aaagtaggcg ctccaactgt tgcaggtgca actgttgaag
     5161 gtactgttga aaaacacggc aaacaaaaga aagtcgttac tttcaaatac aaacctaaaa
     5221 aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt atcgacgcga
     5281 tcaacgctta atagcgtttg agaaagaagg cctatatatg atcaaaggaa catttaagcg
     5341 aaatgacgca ggtcagatcg tttcattcac tttgaccggg catgctgatg ctggtcctta
     5401 tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg tcaacgggat
     5461 tgcctcatta gccggttttg agccgattgt tgaaatgaat gaagaagaag gcggttatct
     5521 ttataccgaa gtcacttcag gaatgacaca agaacaaaac aatattgctg agattctatt
     5581 agaaaatctt ttattaggat tacaatcgat agaagctgaa aactcagaat atatccaaat
     5641 taaaacaatt aatgataaat aggaggtgca gaccatgttg ttatctatga atctacaatt
     5701 atttgcccac aaaaaaggtg gcggttctac atctaacggc cgtgactcag aatcaaaacg
     5761 tctaggtgct aaacgtgctg atggacaaac agttacaggt ggatcaattc tttaccgtca
     5821 acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg acactttatt
     5881 tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa aacaagtgtc
     5941 tgtttatcca gtagctcaag aagcttaatt gaatcttatg attaaagagg ttgtgacaag
     6001 acttttgtca cagccttttt tgtttataaa aataaaagtt gtagtggagg ctacgaagtt
     6061 tactttgtct ttttacactt aatatttttt tgccaatttg gaatcaggat gttttgataa
     6121 ctatcgtttt caattgatgg aagctatatt gtttgctatc agtgaaataa ttatatggta
     6181 aacagtgttc tttttcttgt ttccattata aaaatcgtat cgggattttt cgttctttta
     6241 aattgaacat ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt aaatagttga
     6301 cgaaacagaa ataaatacat agtattatgt tttgaagcct aagtacaata gtctatagaa
     6361 aaggagcgat accattggca aaacgcattg accgaattta tatgtatgtc aaagaaaaaa
     6421 ccgctcattt aacaaaggca gaatatgatc aaggagtaac aacacaggaa attgctgaag
     6481 tgttagggat tcagcgaaca aatagcagta aagatctgaa tcagctagtg cgcgaaggaa
     6541 aactgataaa gacagacgga cgaccagtac gttacatata tcaaacactc gttacgcacg
     6601 taaagccagt tacaaaacat gtagtcagtt acaaagaaac gccggtctct gaagaaaaaa
     6661 atacactcca aatagatact aaggatatct ttgcaaaaat cattggagct aacggcagca
     6721 tgaaaaattc agtggaacag gcaaaagctg cgattttgta tccgccaaga ggattaaatt
     6781 gtttgattac aggacctacc ggatcaggaa agacttattt tgcacacgcg atgtatcatt
     6841 ttgcaaaagc gcatcatgta atagatgagg aaacggaact gattgttttc aattgtgctg
     6901 attatgccaa taaccctgag cttttgatga gtcatttatt tgggtacgca aaaggtgctt
     6961 ttacaggcgc agatgaagaa aaaatgggga ttatcgatca agcggatggc ggtatgctgt
     7021 ttttggatga gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt tactttatgg
     7081 atcatggaac ttacagtcgt cttggggaaa caacgaaatc ccatgaagca aatgtacgga
     7141 tcgtaggtgc gaccacagaa gacccaggtt cttctctatt agaaactttt gttcggcgta
     7201 tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag aaagtcgatt
     7261 tagtaaaaat catgattgcc cacgaagcaa accgcacgca gcgaaagatt tctttgacag
     7321 aagatgtagc aaaagcattg atcggcagtg tgacctatgg aaatatagga caattgaagt
     7381 caaatatcca gttagtatgt gcacgaggat ttctaaatca tatgcaatca cccgaaatct
     7441 cgatcacaat cgatgatttg acagaaggga ttcgtagcgg attgattcaa ttagcaagca
     7501 accgcagtgc aatggcagag ctttctaaaa tattggagcc aaagataact gtagcaccaa
     7561 atgatacgat catgaaaatc caatcggact cctatgagtt accttataat ctatatgata
     7621 tcatcggaga taaagcagct ttactgaagt cggatggttt ggatcaagaa gcaatcaatc
     7681 atttcatttc tacagatatc aacatccatc tgaagtcttt ctataaagac catggttttt
     7741 cttttaatgc agacagcaaa ttagcagaat ttgtagatcc gaaaatcata gaagtgacca
     7801 atcagattta tgtaatggtc aaaagatctt taccatatga gtttcagcaa aactttattt
     7861 atgctatgag cttgcatatc agttcttttt tgaaaaaaat caacttagga gaagaacggc
     7921 atacgaatga taacatccgt gagatggctt ttgattttcc agatgaatat gctgttgcga
     7981 aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga gattccagaa agtgaagact
     8041 attacttgac cgttctactc gtctcgttac gggcaaatca agcaagcggc agaatcggtg
     8101 tagtcgttgc agctcacgga aacagcacag caagcagtat ggtccaagtt gtccaacagt
     8161 tgttagatgc agagaacgta cgagcagtag acatgccttt ggatatggat ccgaaaacag
     8221 cattggaccg aattgaacgc aatgtacaag aagtagatga aggaagcggg gtcatactct
     8281 tagtagatat ggggtccctt gcgtcgttta attcgcaaat tcaaagagac actggaattc
     8341 cagtacgtac tgttgatatg gtaacaactt ctctagtttt ggaaactgta cgaaaggtct
     8401 cagtattagg gacagatcta gatatgttgc atgaatcatt gaaaaatttt cgaggttacg
     8461 cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc tattcataag aaaaaagctg
     8521 ttttggctat ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa ttgatcgaac
     8581 gagcggtaaa caagcgtcaa gaaacagaat tagaagttct agctttgtcg attgtggaag
     8641 tgaaagaaga actgccagtt atccaaaaag aatatcagat catagctact actgggatta
     8701 ctgatttgaa tatccaagcc ccatttattc ctttagaacg ttttattgat caaaatatcg
     8761 aagtcatttt ggatcagttg ctgatggaat cagaattgga ggaaactgaa ttcatctccc
     8821 tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat ctctgataac ttcatcttta
     8881 ttaatggtag taagttaatc gatccaatgt ggcaattttc tacacaaatc tctcagacga
     8941 cgggaatcgg cgatgaagaa tatggattca aaatcaacct agtgatgcac acagcaggaa
     9001 tgatcgaacg tattattcgg aatgaaccat tgactgtgga agaaaacgaa ttgacaaata
     9061 caacgaatga tcctctttat agccaattgg cagcctcagt tgttctgttg gaagatcaga
     9121 taaaagtaaa agtgccgata gaagaaatgt attatctgct acgtctagtc cataatcagt
     9181 tagataaaaa agaatacact gtgccatgaa tacacaaaaa cgatacactt ttatagtgta
     9241 tcgtttttgt gtattggtta agaatccttt aaattcaatt agtgtatcgt aaaaaagttg
     9301 gcacgctttt tgcttatagt agtgtgtgct aggaggtggt tgttcacaca gaaatgatag
     9361 gggtttctgt gaagtagtga ggaagaagga gttttgcaga tggatattcg attggcacgt
     9421 atagatgatc gtttaatcca cgggcaagtc gcaacagttt ggtcgaagca aacaggtgtt
     9481 gaacgtatca ttgtgatcag cgatgtagtc gctcaagata aattgagaaa gtttctacta
     9541 aaagaagctg caccgccggg gatcacagca aatgtaataa ccgtgcagaa aatgattcaa
     9601 atctatcata gtggattatt atcacgagca aaagtgatgc ttttattcac caatccccaa
     9661 gatgttgaag cggttgtccg ctgcggtgta cagttgaagt cgttgaatat cggcggtatg
     9721 agttttactg aaggcaaatc aatgattacc aattttgttt cagtaaatca acaagatatt
     9781 gattcctttc gttacttgaa cagtcaagaa gtggagttag agattcgaaa agtacctgct
     9841 gacaggaaaa tccttttgat ggaactacta gcgaaaatca aaaaggtgta gcaagaatgg
     9901 ttgttggaaa aggtcaaata attctgttgt aatggttagc aattattaat atttttgaat
     9961 ttgttacaac aaaagtaaat aggaggtata aatatggtag ggattatcct agcaagtcat
    10021 ggacaatttg ctgaaggaat cttacaatcc ggttcaatga tctttggcga acaagaaaac
    10081 gtcaaagccg tgattttgaa accaagtgaa ggtccagatg acttgagagc caaattggaa
    10141 gaagcggttg cttcttttga taatcaagac gaagtgttat tcttagttga tttatggggc
    10201 ggaacaccgt tcaatcaatc aaacacatta ttcgaagagc acaaagataa atgggcaatc
    10261 gtcagcggtt tgaatttacc aatgttgatt gaagcctacg catcacgctt ttcaatggaa
    10321 tctgctcatg aaattgcagc acacatcatt gaaacagcaa aagacggtgt aaaagtaaaa
    10381 ccggaagaat tagaaccggc agaagcaccg aaagcagcag ttgaagacgc acaacctaaa
    10441 ggagcacttc cagaaggaac tgtggtcgga gacggaaaaa tcaaatatgt attagcacgt
    10501 gttgactcac gtctgttaca tggacaagtt gcgacagctt ggacaaaagc agtgcaacca
    10561 aaccgtatta tcgttgtttc agatgcagta tcaaaggatg atctgcgtaa gagactaatc
    10621 gaacaagctg ctcctccagg tgtaaaagca aatgttattc ctattagcaa aatgatcgaa
    10681 gtagcaaaag accctcgttt tggaaatacc aaagcattgc ttttatttga aaatcctgaa
    10741 gacgtattga cagctgttga aggtggcgta gacatcaaag aattaaatgt tgggtcaatg
    10801 gcgcattcgg tcggtaaagt cgttgtaagt aaagtattgt ctatgggaca agaagacgtt
    10861 gaagcgtttg agaagcttga acaaaaaggt gtgaaattcg atgtacgtaa agtacctaat
    10921 gattcacgcg ataacatgga tgatattttg aaaaaagcaa aagctgaatt agcaaaagcg
    10981 taataaataa tattaaaatt tataggaggc ttatcatgtc tattatttca ataattttag
    11041 tcgtattagt tgcctttcta gctggtatgg aaggtattct tgatgaattt caatttcatc
    11101 aaccactagt ggcatgtaca ttgatcggtt tagttaccgg taacctcgaa gctggtatcg
    11161 tacttggcgg tacgcttcag atgatcgctt taggttgggc aaatatcggt gccgctgtag
    11221 caccagatgc tgcattagca tcagtagcat cagcaattat tttagtttta ggtggacaag
    11281 gtgttaaagg tgttccttct gcgatcgcga ttgccgttcc tttggctgtt gcagggttgt
    11341 tcttaacaat gatcgtacgt acaatcgctg ttccaatcgt tcatttgatg gatgcggctg
    11401 ctgaagaagg aaacatccgt aaggttgaaa tgtggcacat cattgctgta tgtttacaag
    11461 gggttcgtat cgcggtccca gcagcagcgt tgctatttat cccagcacaa actgtgcaat
    11521 cattccttga atcaatgcca gcatggttaa cagatggtat ggctatcggt gggggaatgg
    11581 tcgtagctgt aggttacgct ttggtaatca acatgatggc tacaaaagaa gtatggccat
    11641 tcttcgttat tggtttcgtt gtagctgcaa tttctcaatt aacattgatt gctctaggtg
    11701 ctttaggtgt tgcattagct cttatttact tgaacctttc taaaatgggt ggttcatcaa
    11761 ataatggagg cggaagcaac tctggtgacc ctttaggcga cattttgaat gactattaat
    11821 cttgaaggag gaaaataaaa tggcagaaga aaaaatcaaa ttatcaaaaa aagatcgttt
    11881 agctgttgcg tggcgctcta ccttcattca aggttcatgg aactacgaac gtatgcaaaa
    11941 cggtggctgg gcattttcta tgattccagc aatcaaaaaa ttatacaaaa caaaagaaga
    12001 acgttctgca gcattaaaac gtcacttgga attctttaat acacacccat acattgcttc
    12061 tccaatttta ggggttacat tggctctgga agaagaacgt gcgaatggcg cgcctgttga
    12121 tgatgtagcg attcaagggg ttaaagtagg tatgatgggt cctttagctg gtgttggtga
    12181 cccagtgttc tggtttacag tacgtccaat gctaggtgca ctaggtgctt cattagctat
    12241 gggaggaaat atccttggac caatcatctt cttcttaggt tggaacatta ttcgttgggc
    12301 attcatgtgg tatacacaag aatttggcta caaagctggt tctaaaatta ctgatgacct
    12361 ttctggcgga ttgttacaag acgtaacaaa aggtgcttca attttaggga tgttcgtatt
    12421 agctgccctt gtacaaagat gggtatcaat caaattctta ccagttgtat caactgtaaa
    12481 attagataaa ggtgcttaca tcgaatggga taaattgcct tcaggtggtg aaggaatcaa
    12541 gagtgcattt gaacaagtta ataatggctt agccctttct cctgaaaagg taacaacatt
    12601 acaagacaac ttagaccaat tgattcctgg tttagctgca ttaggattaa cattgttatg
    12661 tatgtggttg cttaagaaaa aggtttctcc aatcgttatc atccttggtt tattcgtagt
    12721 cggtgttgtt ggacacgtca tcggactttt ataatccata aaaatatagc tcgggcgttg
    12781 tcccgagctt tttttatata gtatactgtt cataaacaaa tatctggagg gagaaaatgg
    12841 ttcaatcaat caatacaaaa gtagacttgg taattgatgc tacttcgttt acgggtctga
    12901 cagattacgg gcaaatcatg ataggtaata atggttttga gttttaccat gcgcgagata
    12961 gacgcaaata tatccaaatt ccatgggaag aagtggatta tgtactagca tcagtcatgc
    13021 taaagggaaa atggattcct cgatatgcca ttcaaacgaa aaaaaatggt acatacacat
    13081 tttcttccaa agaacctaaa aaagtgcttc gtgcgatcag agaatatgta gatgaagata
    13141 gaatggtaca atcattgagt ttctttgatg tagtgaaacg tggtattcgt agtatgttta
    13201 aacgcaagta aaaaataaag aaaacttcta agaggaggtt ttctttattt ttttactttg
    13261 cagaagaaaa tatgaaatag caaaaccaat tatttgtccc ctttaaatcc catatgctaa
    13321 aattgattag caaagaagga ggttatatta tgagttggtt atggacattg attgttggag
    13381 ccgtgatcgg agcaattgca ggtgcgatca ccagtaaagg aaattcgatg ggctgtgtaa
    13441 cgaatatcat tgctggacta gtcggttcaa gtattggaca agcgattttc ggcgaatggg
    13501 gaccaaaagc tgcaggtatg gcacttattc catctatttt aggcgcagtg attttggtag
    13561 cagttgtctc atttttcttt ggaaaacgat cataagtttt tacaaaatag taaactaaaa
    13621 ggagttgcag cgagactgtg actccttttt ggctttattt atttaaaata aaaagaagtt
    13681 ttccggaaat ttttatttaa ctgtcgtgta cgaagaaaag tttgctataa tggaaaaaag
    13741 cgtgcaaaga aagagggagt ttcattatga tgctacgtgt agaaaaattg cgcaaaaaaa
    13801 tgcaagaaga aaatttggat tcatttttgg ttaccagtcc gtacaatctt cgttacttaa
    13861 cgaactttac aggtacgaca ggattagctg ttattacttt ggaaaaagca ttttttatta
    13921 ctgattttcg ttataccgag caggcggcag cgcaggctca agggtttgaa attattaaaa
    13981 atgtcggacc tatttttgat gaggtagcaa acttagtacg caaagaagaa ctgcactcat
    14041 tagcttttga ggaaacaaca gtctcatttt tagaatattc ggttttagaa gaaatcatcg
    14101 atgcggaatt agtacctgtt tcacagatga tcgaagaact cagagaagtg aaagatgaag
    14161 aagaaatcgc tattatcgaa aaagcatgtc atattgcgga tatggcatat gatcacatcc
    14221 tgaaaatgat ccaaccgggc atgactgaga ttgaagtcgc aaatcaacta gacttttata
    14281 tgcgttctct tggcgcttca ggtgtttctt ttgatacgat cgttgctagc ggattgcgtt
    14341 cggctatgcc gcatggtgta gcaagcaaaa aaatcatcga acaaggtgac ttgatcacat
    14401 tggactttgg ttgctattac gaagggtacg tttccgatat gacacgtaca ttcgctattg
    14461 gtgatcctgg ggaaaaatta aaagaaattt atcaaatagt cttagatgct caattagcag
    14521 ttattgatgc tgcaaaacca ggaatgactg gtgtccaatt agatgcagtt gctagagatt
    14581 atatttcaaa acatggttac ggggaagcat ttggacattc tacaggacat gggatcggtt
    14641 tagagatcca cgaaggacca aacgtttctt ctcgtgcaga aaagcaattt gtaccaggaa
    14701 atgtgatcac tgatgaacca gggatctact taccaggaat cggtggagta cgtattgagg
    14761 atgacttgtt gatcacagaa gatggaaatc gtgtgttgac tcgttcacca aaagaattga
    14821 tcatcttata gtttcatcaa tagaagaagt aagcgtaaaa atcagtgttc acagactttg
    14881 ttatagaaaa tcactcgatt taatgataca ctatagaaga acttttaagc tgtaagagat
    14941 aataaagttg ggtgttttct ccaattcata gcagtttgca gctgatcggc ggcagttttc
    15001 ttaccacgtg atcactatgc cgcatcatag agaattcgga tgtatctcgt gcttaggcaa
    15061 agggaggaat aaataaatgg cagatgaaaa aaatttgatt ttaaacgcaa accaagattt
    15121 aggagagatc gtgatcgctc ctgaagtcat cgaagtaatt atcgggattg cagcttctaa
    15181 agtagaaggc gtatacggaa tgcgcggaac ttttgccagc aatgtcactg aattattagg
    15241 acgtgcagct cacggaaaag gtgtttactt agcaaacgag gaagatggct tgaaagtaga
    15301 tctttactgt tatctagaat atggtgtttc agttcctaaa gtagcaatgg aaatgcagga
    15361 acgggtcagc cagcaagtcc ttttcatgac ggatatcgat ttatctgaag tcaatatcca
    15421 tgtagtagct gttgttcctg aaaaattaga acaaccaaca atcgaagaac tttttgaaga
    15481 cgaagaggaa gagaatgaat aaagaattat caagacacga gattcgggaa atggcattgc
    15541 aggctttatt tccattagac ttcaatgcag acctaacaaa agaagatgcg attttcaacg
    15601 caatcgaatt agaccaccgc gacatgatca acgaagatga atcagagttt gttcctgttt
    15661 atcttgatac gctagttggc ggtgtctgtg caaaaaaaga tgaattggat aaagtgattg
    15721 aaaaacattt aaaaaacaat tggcacatca atcggatctc aaaaatggat ttggtgatct
    15781 tgcgtatcgc gatttttgaa atgacttatg tgacagatgt accagcggct gttgctttaa
    15841 atgaagcaat cgagttggca aaaacattta gtgatgaccg ttcacgtaaa ttcgtcaacg
    15901 gtgttttatc caatgtattg aaagaattag aagctggggc gtaagtccta gcttttttct
    15961 atgaatcatc ataaacctgt tataaatcgg gaaaattcta tggtagaatg aaaacacaaa
    16021 aaatatctgg gagtggttag atggcagaat tgattaacgg aaaagaatta gctgaaaaaa
    16081 tgcaggcaga gattgctgaa aaaatccaaa aattgaaaga taatggtatc catcccggat
    16141 tagtcgtttt attggtaggg gaaaatccag ccagccaaat ctatgtaaga aataaagaac
    16201 gtgtagcaaa agaaatcgga atccattcat tggtagagcg ttatcctgag acgatttctg
    16261 aagacgaact acttgcagaa attgaaaaat acaatcaaga tcctagtttt catggtatat
    16321 tggtccaatt accattgcca aagcatatag atgaagaaaa gatcctgtta gctatccgcc
    16381 ctgaaaaaga cgtagacggt tttcatccga tgaatttagg cagactcctt gcgggtaaac
    16441 cggacaagat tccttgtact ccatacggca tcatgaaaat gtttgaagct tattctatcg
    16501 atccggcggg taaacgagca gtagttatcg gacgaagcaa tatcgtcggt aaaccgatgg
    16561 ctcagctact cttgatggca gatgcaactg ttactgttgc tcattctaaa accgaaaatc
    16621 tggctgaact ggcacaagaa gcagatattt tagtcgtagc aataggacgt ggacattttg
    16681 tcacaaaaga attcgtaaaa ccaggtgctg tagtgataga tgtagggatg aatcgtgatg
    16741 ctaacggtaa gctgatcggt gatgtgaaat ttgatgaagt ggagcctatt gcaggctgga
    16801 tcactcctgt gccaaagggt gtagggccaa tgactatcac gatgctgatg tatcaaaccg
    16861 taaaatctgc cgaagctttt ggagcaggtg aatagatggc acaagaatat ttaactgtca
    16921 ctacattaac gaagtatttg aaaagaaaat ttgatgccga tccttattta gaacgcgtat
    16981 atttaacagg cgaaatatcg aatttccgtt tacgtgccaa tgcccatcaa tatttcagct
    17041 taaaagatga ccatgcaaaa atatctgcta ttatgttcaa aggagcattc caaaaattac
    17101 gattccagcc aaaagaagga atgaaagtcc ttgtcgttgg gcgtatttca ttatatgaag
    17161 cgggaggttc ttatcaaatc tatgtagagc acatggagcc tgatggaata ggtgctttat
    17221 atcaagaatt ggaagaacga aaagaaaagc tgatgaagga aggccttttt caagggccta
    17281 aaaaaatgct gccacgtttc ccaaagcgaa tcgcggtcct taccagtcca agcggagcgg
    17341 ttatccgcga tatcatcacg acggtgaaaa gaagatatcc aattgtccaa ttggttttgt
    17401 tcccaacagt tgtacaagga aaccaagcag cggacaatgt tgtcaggaat atccagagag
    17461 tagaagagat gggcaatttc gatacaatga tcatcggtcg tgggggtggt tcgattgagg
    17521 atttatggcc gtttaatgaa gagcgggttg ctcgggcgat ttatcaagct aatacgccgg
    17581 tgatttcatc tgtaggacat gaaacagata ccacgatcgc tgatttagta gcagatgttc
    17641 gggctgccac tccaacagca gctgcagaat tagctgttcc tgttttgtca gaagagttga
    17701 tgcgcattca agaacggcaa gctagattgc agcaggcgtt tgtacgccag atccagcgca
    17761 aacaagaacg gttcgagcgt gctcgacgtt cctatgtatt tcggcagcct gaaagattgt
    17821 atgaaggtca gaccatcaaa ttagaccagt tgaaacagcg attatatcaa gctacacagc
    17881 aaatttatca tgcgaaagaa aaacaaacga tcgcattaag ccaccaattg gaacaagtgg
    17941 ctccgcttta tcgagttaaa gcagctcgcc aagaaactgc ttatctagaa aaacgcttag
    18001 aagagaaaat ccaacaatat atgaaacaac agcagcagcg ttttgctcaa gctgttcaat
    18061 ctcttgattt gttgagtcca ttgaaaatca tgggaagagg ctacagctat acgacgttaa
    18121 atgatcaagt agtaaaaagt gtcaccgaga ttcatcaagg ggatttgctg acgatccatt
    18181 atcaagatgg aatcgttaaa ggacaagtac aagagataga caaaggagaa taaaaatatg
    18241 gaaaatccaa cttttgaaga gtcactgcaa gaattagaaa aaattgtcat gcagttagaa
    18301 caaggagacg ttccattaga atcagctcta gattcgttta aacgaggaat ggaattaagc
    18361 aaacattgcc aagatacatt agctaaagca gaagagacct tgacgaaaat gatgacagaa
    18421 tcaaatgaag aagttgattt tgacggaaac gaggaaagct gagatgagat taactgactt
    18481 ttcagctatt catctacctg ctgtagaaaa agaaatcttg tcttttttag atgagcatac
    18541 aacggataga ggactctatg atgcgatgag ttattcagtg aaagcaggcg ggaaaagaat
    18601 acgtccctta ttacttttaa cggccgttgc ttcctttgat gagcctattg atgttcccgt
    18661 ctatcaagta gcagctgctt tagaaatggt gcatacgtat tccttgatcc acgatgattt
    18721 gcctgcgatg gacaatgatg atcttcgtag aggaaaacct accaatcata aagtattcgg
    18781 cgaagcatta gctatattgg ccggagatgg tttgcttaca ggagcattcc agctaatcag
    18841 tatggcacac ttaggtaatt ctcctaagct tctgctgctt cagcaattag ctgtttgtgc
    18901 gggatcacaa ggtatggttg ctgggcaagc tgcagatatc gagggcgaat cgaaaaagct
    18961 ttctttagaa gaattagcat ttattcatga aagaaagaca gggcatttga ttcgttatgc
    19021 cttgctggct ggtggcatat tagcgaagca acctgaagaa atcttattgc tacttcaacg
    19081 attagccgaa catttaggtt tagcttttca gataagagat gatctactcg atgtgattgg
    19141 tacaacgaaa acattaggga aaacagctgg taaagatgaa cgtatggaaa aaaatactta
    19201 cccgcgttta ttaggcctag aaaagacacg tgaggcgttg gagattgagc tgttatccgc
    19261 caacaagatc atagacaaat tagaagagga cgttttttct tttgatggcg ggctgatgcg
    19321 ccagatgatc aagcaatttg atgtggagaa atgagggaaa gaatggaaaa agaacgcgta
    19381 gatgtattag cggtcaaaca aggattattt gaaacaagag aacaagccaa acgttcagtg
    19441 atggctggtt tgatttacaa cgaaaaaaac gagcggtttg ataaaccggg agaaaaaatt
    19501 ccagtgagta gtgaattaag agtcaaaggt aaaaaacttc cttatgtttc tcgaggggga
    19561 ttaaaattag aaaaagcact caaagcattt gatctatctg ttgaaggaaa aacattgtta
    19621 gatattggtg catccactgg cggatttact gatgcagctt tgcaaaatgg cgcaaaaatg
    19681 agttatgctt tagacgttgg ctacaatcag ctagcttgga aaataagaca agatcctaga
    19741 gtcgtcgtaa tggaaagagt gaattttcgt tatgcaaaac ccgaagactt taccttagga
    19801 attccagaag ttgctgttat cgatgtttct tttatttcac taaaactgat gctgcctcca
    19861 ttacatgcta ttttaaaaga agagggagaa gtgattgctt taatcaaacc ccaattcgaa
    19921 gcaggcagag aagctgtagg gaaaaacgga attgttcgtg accctcagac ccatcaaaaa
    19981 gttttagaag acattttgtc atttgcggca caaggtggat atgatgtgct ggaattaagt
    20041 tattctccga tcacgggggg agaaggaaat atcgagttct tggctcactt gaggaaagta
    20101 cccgaaagcg gaacaatcaa ttcagctatt aatatggcag aagtggtttc aaatgcacac
    20161 gaacagtttg accataaata aattgtaact gtgaaaatca agtgagaagt atgcggattc
    20221 ttctttatta taccaaggaa ataagctatc attgattaag aagaatatgc taaggagcga
    20281 agacgtaatg agaaaaaaag atcggcatcg tttgattact cgattgttga gtgagcaaga
    20341 tatccgaaag caggaagaat ttgttgaaat cctgaaaagc aaaggaatat ctgttactca
    20401 agctacgatt tccagagata taaaggaact gaaactgatc aaggtaccgg cttcagatgg
    20461 cggctaccgt tatagcatac cagctgaaac aaacgaagat gtaaatacta aattggaaaa
    20521 actattaaaa gacgctttcg tggcagttga ccaaatggaa aaatttgtta ttttgaaaac
    20581 attgccggga aatgcttcgg cagcagcaaa tttgattgat aaacgttaca agaaagaatt
    20641 attttcgatt atcaatgatg acgataatgt tttgatgatt acacgaactg aagaccaggc
    20701 agttttattg aagaaagact tcctgcatta cctttaagtt gaggagcgaa tgtgatgcta
    20761 caagaaatca gtattactaa ctttgcgatt attccggaat tgcggctttc ttttcacgaa
    20821 ggaatgaccg cgctgaccgg agagactggt gcgggtaaat ccattattat cgatgcttta
    20881 ggtctgttag caggaggaag aggttcaagc gattatatac gtcaaggagc agaaaaatgt
    20941 gtgcttgaag ggttgtttga actaccaaaa caagaaggat tctctgaact gatggttgaa
    21001 ttagggatcg aaacagatga ggacaatctg attgttcgac gggatatgtc cttaactgga
    21061 aaaaatgttt gtcgagtgaa tggtcatatt atcacattag ctaatctgag aagaatcggc
    21121 agttatttag tagatatcca aggacaaaac gaacatcagg aactgctcca gcctgaatca
    21181 catcttgctt tattggatcg ttttggagat gtagctttcc aacagaagaa aaagagttat
    21241 cagcaagaat atatctctta tcgtgagctg gaaaaaaggg tacgaaaagt gcagcaaaat
    21301 gaaaaatcct atgttcagag aatagatatg ctccatttcc agcaagaaga aatagctgct
    21361 gctgatttgc aagtcggaga agaagagaaa ttaaaagaag aaagagaaaa acttagcaat
    21421 tatcaaaaaa ttgctgatgg attagctgca ggttacggag cactgacgaa taatgaacaa
    21481 aacagtgtag atggtgttgg cttggccgtt tcagaactgc aagggatcgc ccatctagat
    21541 gtggagtatg aagcaattta tgaaaatatc caaagtgctt actatttgct gcaagatgca
    21601 atcggcgata tgagtcgtca gattgattta ttggagttag atgagagtcg tttagaagaa
    21661 gttacgcaac gtttagaatt aatccgacag ttgaaacgta aatacggcga atccattgag
    21721 tcaatattgg cttactatga cgaaatcacc gaagagctag cttcttctga tttttcagaa
    21781 ggtcaattgg acaaaatgaa agaggaacta gagcaaaaag agcttctact tcagcaacaa
    21841 gccgcagatc ttcatgaagc gcgtaaaaaa atcgcaaaag aattggaaaa atccattctg
    21901 catgaattga aaagtctgta tatggaaaac actgagtttg aggtacgttt cttaaaagaa
    21961 gaaaatagac aattgaatca tgatggattt gaccaaatcg aattttacat tacgaccaat
    22021 ccaggagagc cactgaagcc tttggtgaaa gtcgcttctg gtggcgaact gtctcggatg
    22081 ttgcttgctt tgaaaactat tttttcttct gagcaaggtg tgacaagtat tatttttgat
    22141 gaagtggata caggtgtgag cggaagagtt gcacaggcca tcgcagataa aatttctcaa
    22201 atatcgaaat actctcaagt tttgtgcatc actcatctgc ctcaagttgc tgcagtcgcc
    22261 gactatcaat attatattgt gaaagcagtg attgatggac gtacgcaaac ttctgtttca
    22321 gagttgaaaa caaaagaaag agaagaagaa attgcacgta tgctggctgg aagtgagatt
    22381 acaaaactca cactcgaaca tgcaaaagaa ctattgaaac tcgcaaaaag gacctgagcc
    22441 acctcgcagt ggttcaggtc cttttcagca gctgatagct atattttatt tttacatgac
    22501 actcattaca gcagtcaata caacagatcc aatcgttaaa atcagcattg tccaaatgac
    22561 aaattttgtt actttagcaa aagtacttgt ttgttttttt tcacgcattg ttttcaacct
    22621 cgtcattaat tagttgattc aagtgtacaa tcttttttcc atttccacaa gagggctttt
    22681 ttataaaaac ttcccttttt ttaaataata ggtagttacg atacaaataa ataatgttaa
    22741 tagaactagc catataaagg catcatccct ttcagcaata ggtaacttaa tgttcattcc
    22801 atagatactg ccgacgattg tcggtatagt taaaataatc gttaaagaag ttaaaatttt
    22861 cataatatta ttcagattat tcgatacgat tgctgaaaaa gtctgattca tttgttctac
    22921 tagttttaat tgaatgcgag aagtagtcat tgcctgctct gtttccacca atacatcacg
    22981 taaatgattt ttcaaagcat gattttcttg gaaaagttta ttttgcgcta gttctttcaa
    23041 tgcatctaaa ttagcagaag tcgcttctct gaaataaacg aggctttttt ggatatccat
    23101 gatttgataa agttgtttgt tttcagtgga tacttgaagt ttggcttcca aagtatcgca
    23161 ttgagattga agcgtagcta aataactgtt gtaagagagt gtgacctgcc aaatgaattg
    23221 aaagaagata ttgagattag gcgaaatatc actttttgga aaggtctggc taaagacttt
    23281 ctttaaaaac agtggttcat gattcgtaat tgtgataagc ttctttttgg gcgtcaaaat
    23341 aatagagagc gggtatgtat ccacttgaaa ataaccgcta ggacttgttt tagcaaaagg
    23401 atattgtaac aataataacg ctggctttgt caactttgat tgattgaatt cttctgtacg
    23461 ggaattttct gcatcgtcca atatggaagt gatgtaatct gacggaattt caaattgatg
    23521 tgtcagctga tgaatctctt cttgattagg acgttctaca caataccaga tcgtttggtc
    23581 agtttccttt ccggtattac gcagtttctc attatctaat gtaaaatatt tgatcatgat
    23641 tttcctcctg ttgcctgttt cttttattat aacggatagg gaattggaat acatggaatg
    23701 tgtttgtaag atatagaata taactgcata aatttaaata aaatgaaaca gttgtgtaaa
    23761 gtatatacag tatacttgaa gcaacgataa aaaaggagtt tacgttcatg ggcacattca
    23821 caccacttat cttgttttgg tatatttacc cggtaatcgt tttgtttggt tgccaatttc
    23881 ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa agcccctgat ttggcgattc
    23941 cttttttgtg gggcggtatg catgcattgt ctagaaatac agggacaatt tctatcttac
    24001 cgtttttctt tatttctgtt ttgctaatgg gaatttgtat tgctgttttc caagcatatt
    24061 attatgaaga aattatttat ccgcgttatt tcaagatgac ttggcgtttg gtttttctgt
    24121 tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat actttcttac ctatgaaaaa
    24181 aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc agcttgattt ttaaaaaaat
    24241 gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa aggtggtaga aagtggggga
    24301 ttgtggtaga ctaattttag ttagtggagg aatggggggc tttcaatgtt gatgggtgaa
    24361 ttccaacata atattgatgc aaaaggacgt ttaatcgtgc cctcaaaact tcgtgaagaa
    24421 cttggcgaaa agtttgttct gacacgcgga ttggatggct gtcttttcgg ctatccgatg
    24481 agcgaatggg aaaatctgga agctaaactg aacgaaatgc cgttggctaa aaaagacgca
    24541 cgtacttttg tgcgattctt ctattcggct gccactgaat gcgaacttga taaacaagga
    24601 cgaatcaata tacccagcac gttaagaaat tatgcagctt tgacaaaagg ttgcgtagtc
    24661 atcggtgtat ctaaccgaat tgaaatttgg gatgagacac gatggcaaga tttttctgca
    24721 gcagcagaag agaactttga cgaaattgct gaaagtatga ttgatttcgg actataagaa
    24781 taggaagagg ctgactaatg actgaaacat ttcaacacta tacagtcatg ttgaaagaaa
    24841 cggtagatgg attgaacatc aaaccggatg gcgtttatgt agactgtaca ctcggcggtg
    24901 ccggacatag tgaatattta ttaagccaac taagttctaa agggcatctg tatgcgtttg
    24961 accaagatca aaaagcaatc gatcacgcca agctgcgttt agcttcttat gtcgaacaag
    25021 gtatggtcac atttatcaaa gcgaacttca gagaattaga agaacgcttg agtgagcatg
    25081 tagataaggt agacggcatt ttgtatgatc taggggtttc ctctcctcag ctagatgaag
    25141 cagaacgtgg atttagttat catcaagatg caccgttgga tatgcgcatg gatcagtcag
    25201 ctcctttatc cgcttatcac gtaattaatg aatattccta ccatgaatta gtaaagattt
    25261 tcttccgcta tggcgaggag aaattttcca aacaaattgc tcgtgaaatc gaacgagtac
    25321 gaaaaacaaa accaatccaa acaactggtg agcttgtcga aatcattaaa caagtcatcc
    25381 cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag aatttttcaa gctgtgcgga
    25441 ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt agaacaggca atccggttat
    25501 tgaaagtcaa tggaagaatc agcgtaatca cattccattc attggaagac agaattgtca
    25561 aaagtatgtt taaagaatat agtacggtac aagatctacc accgggactt cctgtcatac
    25621 ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa accaatcctt ccttcagagg
    25681 aagagctaac agaaaacaat cgatcacgca gtgcaaagtt acgtattgca gaaaaaataa
    25741 gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa tgcaagatta cgaatatgat
    25801 ttacctgaat tggtggatca gccggaacaa caaatgcctg attcttcttc gaatcaagaa
    25861 atcattcttc cacaatcacc ggcaaaacga ttaaagcaga tttctgcttt ggaaaaaatt
    25921 gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg ttttaacagt tatgctgcgc
    25981 acaaatatca gcggagctga aaaagaaatc acgtcaatac aaacagaaat caatgataaa
    26041 aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac tttctcgtac tgaacggatt
    26101 aaacaaatcg cagaagaaaa aggattgtct attaatgacg acaatttaag gaaagtgaag
    26161 taaatgagtc ttaaaaataa aattcggcgg tttatggaaa agaaaaatct aaatccgatg
    26221 aacaatcgca aaaaagtagg tatcatttta tttgctacga gtattggatt gttcttttta
    26281 tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg ttgctggtac ctcattagct
    26341 gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa aagcaaaaag aggaacgatt
    26401 tatgatcgta acggtgtggc gctcgcagaa gatgcctcct cttattcaat taaagcgata
    26461 ctctcaaaaa catatacctc tggggataaa aaactgtatg ttgaagaaaa gaattttgat
    26521 aaaatagcag aaatcctgca taagaatctt tctatagaca aaaaagatgc actgaatatt
    26581 ttagaggacg gcgcaaaaaa agagctgtat caagttgaat ttggtagcta tgggaaaaat
    26641 atctcccaag aaaccaagca aaacatcgaa gcggacatga aaaaagaagg tgtcgctggt
    26701 ctttattttg tcgaccatca ggctcggatg tatccaaatg gcgtcttttc ttctcatttt
    26761 attggatacg cagttccaga caaagatgag aatggcttag ttggtaaact aggtttggag
    26821 tctgcttaca atgatatctt gagcggtaaa gatggtaaga ttatttatca aaaagataat
    26881 tttcaaaatc ctctgccagg tacagtggca gaagaagaaa aagctgtcga cggacaagat
    26941 atctacacga ctttagacag cagactacaa agttatttag aaacactgat ggatcaagta
    27001 aatgaagaat atcagccaga agaattgaca gcagtcctga tgaaagcaaa aaccggagaa
    27061 atcttggcaa tgggacaacg acctactttc aatcctgaaa caatggaagg attgactgga
    27121 aaagatgcta tctggcgtaa tttcttggta caagatagtt atgaaccagg atcaaccatg
    27181 aaagttttca ctactgcagc tgctatcgaa gaaggagaat tcaacgagaa tgaaaccttc
    27241 caatctggaa aaatccaagt agaagatgct acgatcaatg accatgactt tggtgaaaaa
    27301 ggtgtgctga ccatgcgtca agctctttca tggtcaagta acgtcgggat ggtcatactg
    27361 gaacagcgat taggcggacg atggtataac tatttacaaa aactgggatt tggacaaagt
    27421 actcattctg gattagatga tgaagtaaac ggtgctttac ctacattaaa tattgtcgac
    27481 cgagcaatga gtgcttatgg acaagcggta ggtgtgacga atttccaaat gatgaaagct
    27541 ttcacttcta ttgcgaataa cggtacaatg attcaacctc gatatatcag caaagtggtg
    27601 gatccccaaa ctggagaaga gcgaacaact caaacagaag ttctagggca acctttttca
    27661 aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg ttgtcgaaag cgaaaactac
    27721 ggtagtgctt atggtgtata tagtgtacct ggatacaatg tatcggcaaa aaacggaaca
    27781 gcccaaatcg cttctgatac agggggatat caaacaggag atacagccta cctgtattcg
    27841 attgtggaga tggttccttc tgaagatcca gactatgtct tgtatctaac aatgaaacac
    27901 cctaagacat atgatcgaat ggcattggct aaaatcgcaa atccattgat gaaacgggca
    27961 atggatttta aagaaactga agaagattca gatacagaaa caaaaacaga aaaagtatcc
    28021 gtagccgatt atcgtaatct tgaagcggac gttgcagcag ctgatgctca aaaaagtgga
    28081 ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa aacaatctac tgccaacggt
    28141 gatcagttga tctctggaga aaaactgatc ctatatactg gtggtgataa gctgatgcct
    28201 gacgtaacag gatggtcaaa agcagatatc atgaagctgg ggaaaatcct cggtattgaa
    28261 gttagttttg atggagatgg ttattgtgtc aaacaagaac ttgctccata tgaaaaaatc
    28321 actgaagaca aacttagttt tactttagaa gaataagaag agaaatagga gagaaaagag
    28381 catggaatgg acacaagcat tgattcccat tgtcagcagt tgtgcaatga caattgccgc
    28441 aatgccgcta tttattggtt attttcaaat gaaaaagcaa ggtcaggcga ttcgtgagga
    28501 gggaccaaag tggcacaata gcaaagccgg aactcctaca atgggcggct tagtgtttct
    28561 gatcggctct attttgacag gaatttgggt tggagcttgg caaaaacagc taactcctac
    28621 tcttttcatc ttgctttttg tattagcgtt atacggagta atcggctttc ttgatgattt
    28681 tataaaaata ttcaagaaaa gaaacatggg actgaactcg aagcagaaat tgattggaca
    28741 gattctaggg ggaatcatct tttatcttgt ttaccgttct gaaggttatc caggtgtatt
    28801 gaatttcttc ggactagatc tacctttagg aatcatttat ggtattttcg ctctcttctg
    28861 gcttgttgga ttttcaaatg cagtaaatct gacagatggc atcgatggat tagttgctgg
    28921 cttaggaagt atctcttttg cagcttacgg tctaattgct tggcatcagc atcagtatga
    28981 tgtattaatc atttgcttaa gtgttttagg aggtttactg ggtttcttcg tctacaacag
    29041 aaaacctgcc aagattttta tgggagatgt cggttcatta gcgttgggcg gacttttagc
    29101 ggcaatttct ataatgctaa accaagaatg gacattgctt ttgattggtt tgatctatgt
    29161 tatggaaaca gcaagtgtga tgcttcaagt tacttccttt aagcttacag gaaaacgaat
    29221 ttttaaaatg tctccaattc atcatcactt tgaaatgtgc ggatggtcgg agtggaagat
    29281 tgatacagtt ttttggttga tcagcattgt gacttcgttg atcacattat ggatcgtttg
    29341 gtaggagatg acgggaaatg aaaaaaatat caacatatga aaataagaaa gtgcttgtct
    29401 taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt acatgaattg ggcgcacttg
    29461 tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga agcacaagag ctgctttctt
    29521 tagggataaa agtaataaca ggtagtcatc caatcgaatt gttggatgaa gaattttcat
    29581 tgatggtcaa aaatccaggg attccctaca gtcatccttt agtagcaaaa gcccaagaaa
    29641 tgggtattcc ggtaattacg gaagttgagc tagcttacga agtagcggaa tgcccaatta
    29701 ttgggattac aggaacgaac ggaaaaacaa cgacaacaac gatgacagga ctactactga
    29761 atgcaggagc agatcaagga attgcacgtt tagcgggtaa tattgggtat ccagcaagtg
    29821 gtgtagcgca agaagcaaaa tcagaagata aaattgtgat ggaattgtct agcttccaac
    29881 tcatgggaat cacagatttc cgccctcata tagcggtgat taccaatatc tatgaagcac
    29941 atattgatta tcatggtaca agaaaagaat atgtcaaagc taaatggaac cttcaaaaaa
    30001 acatgacaga aaaagattat ctgatcctta actggaatca gtcagaacta caagaattag
    30061 cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa agaagtgttg gaagacggtg
    30121 tgtatgctga cgattactca atatactata aaaaagaaaa aataatggaa atatctgaac
    30181 ttggcgtacc aggtaagcat aatgtagaaa atgcactagc tgctatttct gtagcaaaat
    30241 tgtatggtat atcaaatgaa gctatacgag aaacgttgca ttttttccat ggcgtaccac
    30301 atcgtacgca atatgtagga gaaatccaag gcagaaaatt ttataatgat tcaaaagcaa
    30361 ccaatatttt agcgactaaa atggctctaa gtggatttga aacgtctaag gtcgtgttat
    30421 tggctggcgg attagacaga ggaaatacat ttgatgaatt aattccttca ttaaaaggaa
    30481 tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt gatggatgct ggaaaaaaag
    30541 caggcatcga gacaatagta acagccgatt ctgtagaaca agcagttcct ttagcattag
    30601 aaaacagtac agatggtgat gtagttttat tatcaccagc gaatgccagc tgggatcaat
    30661 acccaaattt tgaaactaga ggaaacagat tcatggaagc tgtgaaccga ttaaaataga
    30721 aaaagtagaa gcgagcgata aaaaatgaaa attttggtta caggcggagg aacaggggga
    30781 catatttatc cagctcttgc ctttgtcaat tatgtaaaga ctcaagaacc gaatgctgaa
    30841 ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa tcgtccctga tacaggaatg
    30901 cctttccata ccttggagat tcaaggattt aaacgaaaaa tttccatgca taacatcaag
    30961 acggtccgat tgtttcttaa gagtatccgt caagcaaaaa aaatcctcag tgattttcaa
    31021 ccagatatcg ttatcggtac tggaggatat gtttccggag cggtagtgta cgctgcagcc
    31081 aaaatgggta ttccaacagt tatccatgaa caaaacagtg taccaggtgt taccaataaa
    31141 tttttaagcc gatatgtgga tcggatcgct ctttcattta aagatgcggc ttcctttttt
    31201 cctgaaaaca aagcagttct tgtaggtaat cctcgagcac aagaagtagc agatactaaa
    31261 aaatcagata ttttgaaaac atttgatttg gatccagaaa agaaaacagt cttgattttt
    31321 ggcggtagtc aaggagcact taaaatcaat caagcagtat cagttttttt aagtgaatat
    31381 attccttctt cttaccaagt tttgtatgct tcaggagata gatattatga agagatccta
    31441 aataaaataa atcctgaaat acaaaatatt agtgtccagc cttatatcaa aaatatggca
    31501 gaggtcatgg cttccagtga tctgctggtg gggcgtgctg gtgcaacttc gatcgcagaa
    31561 ttcacagctc tggggttacc ggcagtgttg gttcccagcc catatgttac gaatgatcat
    31621 cagactaaaa atgcaatgag tcttgttcac gcaggagcag caaaaatgat tgcagataat
    31681 gaactaacag gtgaatcact aagtcagaca gtcaacgaaa tcatgggtga tgaagaattg
    31741 caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc ctgatgcttc aaaacgtctt
    31801 tatgacttag taaaacaaat aatcaaaaaa taaaggcggt ggtcttagtg agtaagaaaa
    31861 aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa caatttgact ccttggcaaa
    31921 aggctaaccg aaaatatctg gcagaacaag agatgaaaaa ccaagagcca aaaaaagaag
    31981 acataacatt agatacaccc ctttcagatt ctgaagaaga aaaaacggtt caagaggaac
    32041 agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt tgaagagatc aagcaaaaga
    32101 aaaacggtgg accatataac ggttcttttt tgaaccggct gcccaattta aaaagtcaac
    32161 ggcacaaagt actatttcgt cggttagctt tgatcatcac tgtattgggt ataccgctga
    32221 tttttttgat ctattatgtt tctccttata gcaaattaca agcagttgca gtatcaggca
    32281 ataagacagt caacagccaa gaaatcatct ctgatacaaa attgtcatta ggagaaaatg
    32341 tttggggaca gtattttcat cgatctactt atatcgatcg ccttaaaaaa gcacagccaa
    32401 gaatcgaaac agcaaatata catttcaaag ggatgaatga attcgatttg gatgtaacag
    32461 aatataaaga gattgctttg atcgcaaaaa acaatcaata ctacccagtg attgaaaatg
    32521 gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa tctccctatc ttggaagagt
    32581 tcaaagactc tgcaaaaatc aaagaattaa caaagcaata caatcagtta tcatcagaat
    32641 tacaaaaagc aatttctgaa atcaagtaca cgccaagagc aagcaataaa aatctgatcc
    32701 aattgaatat gaatgacggc aatcaagtta ttgtaaatat caataatctt gcgaaccaaa
    32761 tgaaatatta ttcgcaagta gccaaagata tggatgaaaa aggtgtgatc gacatggaag
    32821 ttggtatctt ctcgcatcct tacggtagca ctcaagaaac ggaagaatcg ggccaatcgg
    32881 aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa cgaatcaagt gaagaggcag
    32941 aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa cggatcatct gattcaaatg
    33001 attcaagtga aacaagtcaa atagaagata atccaagtac aactccgtca agtttaaatg
    33061 aaaattgata aaacaacttt aaaaagtaag aatgtacttt ctaaaaacca gtaatttatg
    33121 gtagaattgg aagcagtgaa tatatctatt aataagtata ttttaaaacg gacgctagtt
    33181 gttcgcttgt atatggaata gtaagtagga gggaatccca ttcatggcaa aaacaggaat
    33241 gtatgtaggc cttgatattg gaacgacatc tgttaaagtt gtcgtggctg aatatatcga
    33301 cagccaaatg aatattattg gagtaggaaa cgcaaaatca gaagggatta accgaggcat
    33361 tatcgttgat atcgataaaa cggtccaagc gatacaacga gcagtacgac aagcagaaga
    33421 aaaagcaggt atccagatca aaggcgtaag tgtcggtttg ccagcaaaca tgcttgaagt
    33481 agaaaattgc cagggaatga ttgcagttaa cggagattca aaggaaatca ccgatgaaga
    33541 tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata cctcctgaac gtcaaatcgt
    33601 gtcaatattg ccacaagatt ttactgtcga tgggttcgag ggaatcaaag atcctcgtgg
    33661 aatgatcggc gtaagattag aaatgtacgg tctgttgttt acaggaccta aaacgatcat
    33721 ccataacata cgtaaatgcg tggaaaatgc cggattaatc gtcaacgaaa tggtgattac
    33781 tcctttagcg ctgactgaat caattctttc tgacggagaa aaagattttg gtacgatcgt
    33841 cattgatatg ggtggcggcc aaacaacaac ggctgttatg catgataagc aattgaagtt
    33901 taccaatctt gaccaagaag gtggcgaatt tgtcactaaa gatatctcta ttgtattaaa
    33961 tacgtcattt aacaatgcag aagcgttgaa aataaactac ggggatgcat acccagaacg
    34021 gacatcacca gatgaagaat ttccagtaga tgtgattggt cagtcagaac cagtgaaagt
    34081 cgatgaacgc tacttgtcag aaattatttc agctcgaatg gaacagattt tcaacaaagc
    34141 aaaagatgca ttggaccaaa tcgaagcatt agagttgcca ggtggcgtcg tattgacagg
    34201 cggagctgct agtcttcctg gtgttgtgga cttggcacaa gaaatcttcg gcgtaaatgt
    34261 aaaattgtat gttccaaatc acatggggct gagaaacccg gtatttacaa atgtaataag
    34321 tatcgtggat tattcagcaa acttgagtga agtgtatcaa ttggcaaaaa ttgctgttac
    34381 gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa gaaacaacaa acacatatga
    34441 gagctatgaa gcacctgaag aaacaatcta cgatgagccg gaaccaaaag agtccggaga
    34501 aaatgtaaaa aataaaatca agggtttctt tacaaatatt tttgattaat tcatcctggg
    34561 aggaataaga tatggaattc tcaattgata ataatatcaa tgacggcgca gtaatcaaag
    34621 tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg aatgattgaa gaaaacgtta
    34681 aaggtgttga attcattaca gccaacacag acgtgcaagc attaaaaaat tcaaaagctg
    34741 aaacagttat ccaattaggc cccaaatata ctcgtggttt aggtgctggt tctcagccag
    34801 aagttggaca aaaagcggca gaagaaagtg aacaatcctt acgcgaagct ttagacggtg
    34861 cagatatgat tttcatcact gcaggaatgg gtggaggaac cggtacaggt gctgcaccaa
    34921 tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt tggtgtcgtg acacgtccat
    34981 tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga aggtatcgct cgtttgaaag
    35041 aaaatgtgga tacattatta attatctcaa ataaccgttt attagaagtt gtagataaaa
    35101 agacaccgat gcttgaagct ttccgtgaag cagataatgt tttacgacaa ggtgtacaag
    35161 gtatctctga tttgatcaca gcgccaggtt acgttaacct tgactttgct gacgtgaaaa
    35221 cagtgatgga aaaccaaggt acagcattga tggggatcgg tgtggctagt ggagaagatc
    35281 gcgtagtaga agcaacgaaa aaagcaatct cctctccatt gcttgaaaca tctattgatg
    35341 gagctgaaca agtacttctt aacatcacag gcggtttgga tatgacattg ttcgaagcac
    35401 aagatgcttc agatatcgta gcaaatgcag caactggcga tgtaaatatc attctaggta
    35461 catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac ggtcatcgca acaggtatcg
    35521 atgaatcaaa aaaggaacgc aagtctagcc gaccagctag acaagcacaa atgcaatcac
    35581 ctgctcaaaa gactgtatta gatatggatc aagctaaacc aacttcttca gaagaagaaa
    35641 acagttttgg cgattgggat atcagacgtg aacaaaatgt tcgtcctaga gttgatgatt
    35701 ccaattttga caatatcgaa aaaaaagaat ttgatacatt caaccgcgaa gaaacaaaat
    35761 ctcatggcga tgatgaatta aatacgccac cgtttttccg tcgtaaaaaa taaaggagag
    35821 gaagagcaca tgattgctga caacttgcag aaaatcaagc aagaaattca ggattcgtgt
    35881 gctcttgttc atcgtgatcc gaaagaagtt acattagtag ctgtaacaaa atctgttgaa
    35941 tccgctcaag ctgaagaatt ggttagagct ggcgttatga attgtgcaga aaaccgagta
    36001 gataaattat tagaaaagaa acaagattta tttgattttc ctgaaataaa gtggcattta
    36061 attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa atgaaattga ttactttcat
    36121 gcgttggaca gtttaagatt agcagaagaa atccagaagc ggttagagaa agaattatcc
    36181 tgttttattg aagtaaatgt ttctggtgag gaatcaaaac atggaatttc accagatgag
    36241 cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa tcaaggttgt tgggttgatg
    36301 actatggcgc ctaaagaagc ttctgatgag caaatccata aagtgtttgg tacgctgaaa
    36361 gaattgcgtg atcttgttca agcaaaaggt tatgcccatg caccatgtag tgaattaagt
    36421 atggggatga gtcaagactt tcaaattgcg attgaagaag gtgcaacatt tatccgtgta
    36481 ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa atgccgcttt taaacagagg
    36541 agcaattgct aattttttcg gattagctga tgaggaagag tacgaatacc aagattatcc
    36601 tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat acaacacaga atcaatcaac
    36661 tcattctgta tctgctgcga atactcatga aaaacaaaaa acggcgactt cacgtcctgt
    36721 aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta attgaactac atcaatcacc
    36781 tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc tctacacaac ctaaagcaaa
    36841 tcttgctgga aaaatcactg taatggaacc aagagcttat tctgaagcta tggcgattgc
    36901 taaacgaatc attgctggag aagcaacctt agtgaatttt catttagtag aagaaaaaca
    36961 agcgagaaga atcgttgatt ttctaacagg aacagtttat gcactagatg gagatatcaa
    37021 acgtgttgga gatgagattt ttctttgtac accgactggg atggagattg acagttcaac
    37081 cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa gaggaggaac tacgatttat
    37141 actattgcac tattacttca tttacttcaa cgggcagcct atttctatag cattatcttg
    37201 atggtatatg cattgctctc ttggtttcca ggcgggtatc aatcagggct aggacgatta
    37261 ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc gtctgccttt gaggattgga
    37321 cccttggatt tcacaatttt aggggcaatc ctgatcttga acttagccgt acaaggttta
    37381 gcacagatcg ttatatggat aactgtccat ttagtttatt gattgaagca acaaaatctt
    37441 tattctcaga atagatagga ggggaaaatt ttggatgcaa atgtatacca gcactttcgg
    37501 gcagatgaac gtacatttat cgattcggta ggcgattgga ttgaacaggt tcaaggacaa
    37561 tatgcaccct atctgaccga atttcttgac ccaagacaat cctatatttt agaaacactg
    37621 attcgacaaa gcagtgattt gcgtttcatg ttttatggcg gatatgaaca ggcagaaaga
    37681 aaaagatgtt tgattttccc cgattattac gaacctgtag aggaagattt tgaaacagca
    37741 ttgtatgaaa tccattatcc tagcaaattt gccagcctaa gtcatgggaa aatccttgga
    37801 actttagtgg gaacggggat caaacgctct tactttggcg atatcatctc ggacggtgat
    37861 cgctggcaag tatttattgc aaatgaggtg gatcattttg ttgctgcaca agtaacaaag
    37921 attggaaagg ttgctgtacg cttggaacca atcaaataca cagaaatgat tatgccgaaa
    37981 gatagctgga cacaagaaag aggaacagtt agttccatgc gactagacag tgtgatctct
    38041 gagatttata atatttccag acagcggtcc aagcagctga tcgaagctgg taaagtaaaa
    38101 gtgaactgga cagaaaatac aagacctgat tttgtacttg aactattaga tattatttca
    38161 gtcagaggtt atggcagagt ccagatccaa gaacttgagg ggaagacgaa aaaagaaaag
    38221 tacagggtac tgttcggtgt attacgtaaa taaaataaaa aggtggttta ctcatggctt
    38281 taactccatt agatatccaa aataagacat tcccgacaaa aatgcgggga tataatcaag
    38341 acgaagtaga tgatttctta gatttagttg ttcgtgacta tgaggaattg actcaaagaa
    38401 atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa acttgagtac ttcaatgaat
    38461 taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga tacggctgat aaagtaaaaa
    38521 caagtgcaag caaggaatcc gaagtgatcg ttacttctgc acaaaacaaa gcagatgagt
    38581 tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac tgatgcagaa gaaaaagcaa
    38641 gaaaaatctt gacagatgca acagaaaaag caagacagct tgcgacagaa actgaagatc
    38701 ttaagaaaaa aacacgagta ttccatcaac gtatcagctt gatgcttgag tctcaattag
    38761 aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc attctccagc tacgtgactg
    38821 acagccatga agtgatcaaa gaagttttag caaaacagct tgacaatgaa aatgatactg
    38881 atgtaaactc agacacagga gtcatagaag cacctatgac ggcatttgat acacaggcta
    38941 ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta gaacagaaaa gcactttgtt
    39001 attcttaaag cgagccgatg agagtggaag ttcggtaaga cactgcatgg tgaaagatcc
    39061 tctacttgag acctgctttt ccgaaattag agtaagaagc agcggctgac ctcgttattg
    39121 gtcacagaga gtttgcacta gatagatgca actaaatttg ggtggtacca cggcatgttc
    39181 gtcccttgcg gctgaacatg cttttttgtg tccggaaaac aaataaagga gtgtatggtt
    39241 gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa attccctatg cgtggtaatt
    39301 tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga aaatcgaatc taccaaaaaa
    39361 gacaagagtt gaacgaagga aaacctagct ttgttcttca tgatggacct ccatatgcca
    39421 atggtaatat ccatttagga cattcattga ataaaatcag taaagacatt atcattcgtt
    39481 caaaatcaat gtctggctat cgtgcaccat atgttccagg atgggataca catggattgc
    39541 cgattgaaca agtattggct aataaaggcg tgaaacgtaa agaaatgtcc atggcagaat
    39601 acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga taagcaacga gcagatttca
    39661 aacgcctagg agttgcagga gattgggaac atccttatgt tacattagat ccttcttatg
    39721 aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa aggctatatt tacaaaggat
    39781 tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt agcggaagct gaaattgaat
    39841 ataaagatgt aaaatctcca tctatttttg tagctttcaa tgtcaaagac ggcaaagata
    39901 ttcttgatga agatacagct tttgtcattt ggacgacaac accatggaca ttgccagcaa
    39961 accaaggtat tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc gatggccgta
    40021 agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc agaaatcggt tgggaaaatg
    40081 taacagttct aaaagaattt gctggacaag aaatggaata tatgactgca agtcatccat
    40141 tctatgaacg tgaatcactt gtgattttag gcgatcatgt tactttagac gctggtactg
    40201 gattagtcca tactgctcca ggacatgggg aagatgacta tatcgctggt agcaaatata
    40261 aactatctgt agtgtcacct gtagactcaa aaggattgtt tacagaagaa gcacctggat
    40321 ttgaaggtat cttttatgac aaagcaaatc ctatgatcac tgatctatta aaagaaaaag
    40381 gagctttgct gaagctagat ttctttactc atagttatcc tcacgattgg cggacgaaaa
    40441 agcctgtgat ctatcgggca actccacaat ggtttgcatc tatcgataaa ttccgtcaga
    40501 atattttgga tgaagtagaa aaagtagagt gggttatccc atggggtaaa acacgtttgt
    40561 ataatatgat ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca tggggagtac
    40621 cacttccgat tttctatgct gaaaatggag aagcaatcat cacaccagaa acaatcgaac
    40681 atgtggcaaa attatttgct gaacatggat caattatctg gtttgaaaaa gaagcgaaag
    40741 aacttcttcc tgaaggattt acccacccag gttctcctaa tggcgaattt acaaaagaaa
    40801 aagatatcat ggatgtctgg tttgattctg gttcttctca tgaagctgtt ttacgtcagc
    40861 gaccagaatt atcattccca gcagatatgt atttagaagg atcagaccaa tatcgtggct
    40921 ggttcaactc aagtatcacg actagtgtgg ctatcaatgg tgtagcacca tacaaatcag
    40981 ttatttctca aggatttgct ttagatggtg aaggccgtaa gatgagtaaa tctttaggaa
    41041 acatcattgc accagaaaaa gtgatcaaac aatttggtgc agatatcctt cgcttatggg
    41101 tatctagtgt agattatgaa gcagatgttc gtgtttctat ggatatctta agtcaagtgg
    41161 cagaagtata tcgtaaaatc agaaatacga tgcgtttctt attagcgaat acagaagatt
    41221 tcgatcctaa aaaagatact gtttcgtaca atgatttacg ttctgtagac aaatatatga
    41281 ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga agggtacgac aaatataact
    41341 tcatgcatat ttaccgtaca gttatgaact tcttgacggt tgatctatcg tcattctacc
    41401 tggattttgc taaagatgta gtttatattg aagcagaaga tgattatcaa cgtcgttgca
    41461 tgcaaactgt tttctatcaa acagctgtag ctttgacaaa attattgact cctatcattc
    41521 cgcatacagc agaagaaatc tggtcattcc tgaaagaaga agaggagtac gttcagttaa
    41581 gcgagtttcc tggttatgaa gagtttgcaa accaagaaga attgatggat acctggactg
    41641 ctttcatgga cttccgtgat aatgtcttaa aagcattgga agaagcaaga aacagcaaat
    41701 tgattggtaa atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa gttcgtgaaa
    41761 tgttaaaagc tttggatgca gatattgcac aattattgat cgtttcacca gactactttg
    41821 aagttatggc agcagatgaa caagtgccag ataacgctat ggtatttgat gatgttgcta
    41881 ttacagtgga aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt cgtaaagatg
    41941 taggagtaga tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc gttgaagact
    42001 tctatcctga agctgtagct gaaggattcg aagaaaagta aaagggaaaa gggggtgtga
    42061 catgttcttt gtcacacccc cttttgttat caatcgttta attttccacg ttcacgatac
    42121 cagttatctg gttgccagat ccattcgaat ccgtcttttt caagtagttc gaaagctgct
    42181 ttaggtccca ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg gtcccaagct
    42241 tttcgaatga catccacgat atgccaagat tgagcgactt cttcccaatg agagaagttc
    42301 gttccatcac cattcaacgc atctaatagt aatttttcgt aagcttctgg gctattttca
    42361 accatttctg cactatttct aaattccaat ttgattggtt ccgtttcaaa tccttgccct
    42421 acttctttcc cattcaagga taaagaaaat ccttcagttg gctgaatata gatcgttaga
    42481 acattaggtg gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt gaatacatta
    42541 acaggaactt gtttgaatac gatgttgatc cgagtccctt tttcagtcaa acgtttgcct
    42601 gtacgtacat agaaaggaac acctgaccaa cggaagttat cgatagtgaa tttgcctgcg
    42661 acgaatgttt cagtctctga ttgagcatcc actttgtctt cttcgcgata gcccttgaat
    42721 tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc taacacttct
    42781 tcttcgttat aaatgcggat agctttcaat gctttgatct tttctgtacg gatttctttt
    42841 tcggaaaatg ctactggcgg ttccattgca agtaatgcta caacctgtaa aatatgattt
    42901 tgcaccatgt ctttaagtgc gccactatga tcgtagtagc ccccgcgatc ttctacaccg
    42961 aggttttcgc caaaagtaat ttggatattg tcgatatagc gattgttcca ttgagcctca
    43021 aaaatattat tggcaaaacg gatagctgag atgttttgga tcatttcttt tcctaaataa
    43081 tgatcaatcc tgaaaatatc ttgttctggg aatacttctc tgatttgctg gttcaattca
    43141 aaagcagact ggtaatcaga accgaacggt ttttctatga tcaaacgatt gaagccattt
    43201 tttgtcataa tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa ttgaggtgcc
    43261 atagccaaat aatagacacg gttaccgttt aattgatagt gtccatctaa tcggtcagac
    43321 aattctttta atgtctgata atgcgttgaa tcatttacat catgggattg atagtaaaaa
    43381 tggcttgcaa aggtagttgc ttcttcatct gaaggattca atgcacggat tgtttctttg
    43441 acaacttcac ggtagtgctc atcgctccag ggacggcgag cggttccgat tacagcaaaa
    43501 tgctcgccta aatttccttt tctgtaaagg cggaacaaag acgggtacaa tttacgctgg
    43561 gctaggtcac ctgtaccgcc gaatatcgta aacaatacat ttttttcatt agacatgtgg
    43621 atacactcct ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag ctgacgggga
    43681 ttttctttct atcattttgg atagtgatcg gtccttcata agctgcgatg tcaacgatct
    43741 catattcttc gttgatattc aggtcaatag tgactaagta atccaacaat tctttttcat
    43801 caagcactcg tgcgatacgc acttttgtac cgacaggata ggactccaat gtttgtcggc
    43861 gttcttcatg gacaacttcc tcttttttag gaatcgatcc tccatgagga caatgttcag
    43921 gatgattcaa atagttttct aaatgatctg caagatgttc cgaagtcaca tgttccaata
    43981 cttcagcatc atcatggact tcattccagc tatagtctaa atgttcaact aaaaaaactt
    44041 cccataagcg atgtttgcga atcagactgc tagctttttg cagtccgctt gcagtcaatt
    44101 gtacaccttg ataaggggaa tgttctacaa gctcttcttt cacaagttta gaaatcattt
    44161 cactgacaga tgcagctgag acatctagac tagatacgat ttgtttattg ttgactttcg
    44221 ttgtatctcc acctagttct aaaatgattt tcaaataatc ttctcgattt ggtgtcatat
    44281 tcgatcatcc tttcatggta aaaaaccatc attagtaagt gtaacaaaaa aaacgttaat
    44341 tgactatttt tctaaataaa aaacaaagga tttttagacg tttttacaac aaaaaacgaa
    44401 agatgataga gaagactcat tctttcgttt ataaaggata tatatataca ttgggaggga
    44461 tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa agagtttgtt
    44521 cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa cgatattcat
    44581 ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg taatcagatg
    44641 taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct tgatactttg
    44701 agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa cttgagagaa
    44761 cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca ctcctttatg
    44821 agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga ataaaagcta
    44881 aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt atgataggat
    44941 acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat agcttttttt
    45001 attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga aataattatt
    45061 gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc tgaagtggct
    45121 attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc tgtaaaaaag
    45181 aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt
    45241 ttagacggca cgatcctttt accaggatta actggtccat ttacggtata caagcgtgaa
    45301 gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg acaaaaagca
    45361 tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt tcctgatgcg
    45421 cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca agatggttat
    45481 gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac gatccatgaa
    45541 gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc
    45601 ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc cattccattt
    45661 gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat aatgatcaac
    45721 ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc tttaagcaca
    45781 aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca ggaatttggt
    45841 tctcattatg aaggggatat cgaattcgca acagatttga agactttttt caatattact
    45901 catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca agctaagcaa
    45961 gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa
    46021 atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga agcagcaaga
    46081 aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa agacgagata
    46141 atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt attagatgga
    46201 gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga tttaggtgtt
    46261 taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca ttatttccca
    46321 agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta tgagaatttt
    46381 gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga gatttatttt
    46441 gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca atttcatgat
    46501 cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc aatggcagat
    46561 gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg gggattgttg
    46621 tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga tttaagaaaa
    46681 aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac gaatttgtac
    46741 aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc tcaccttctc
    46801 ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta gcggtttctc
    46861 ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac gtggaaccag
    46921 gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt caagtagcag
    46981 atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg gtgggcggag
    47041 ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg attggtacac
    47101 caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa
    47161 tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc attgatcaaa
    47221 ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca gcagatcgtt
    47281 cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac gtgacagcag
    47341 aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata cgaaagaaag
    47401 atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt ttctttaatc
    47461 aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta ccggtaggaa
    47521 gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga
    47581 aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat attacaggtc
    47641 ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg catcgttcgg
    47701 gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa gataataatc
    47761 taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag atttttttat
    47821 acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct gcagtaaata
    47881 aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac aaagaaaaaa
    47941 taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca aggcgcaaat
    48001 aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc agaggagggg
    48061 ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc aacaaggatt
    48121 gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa gccgatattt
    48181 aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag tcgtttactc
    48241 tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta cgcaagtgac
    48301 ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc aggcaaaagc
    48361 tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa ctggtacagg
    48421 gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga tgctttctgc
    48481 agatgcgcca tttgtttctt tcaattgtgc ggattatgat caaaatccgc aactgctctt
    48541 tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag atagtcccgg
    48601 tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc gtttaccgcc
    48661 ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc cgcttggaga
    48721 aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg aatcttcaga
    48781 aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc cgaatcttgc
    48841 ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac aagaagcgaa
    48901 tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca tgctttatga
    48961 tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg caaaatcttt
    49021 tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag aactttccct
    49081 tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg accgattcat
    49141 ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg tctggtcaca
    49201 agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca cttctctttc
    49261 cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg tggatgctta
    49321 tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc ttttgccaga
    49381 tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt tagaccgtat
    49441 ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat tggatcgtgt
    49501 gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa attttaagag
    49561 ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac aagttgagat
    49621 tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag gagaaagtga
    49681 gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac agactgcttc
    49741 aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag cttttaatat
    49801 gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg tcaaaaacta
    49861 tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat taaacacctt
    49921 cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga tgacaagtac
    49981 catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag aagatattta
    50041 tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag cggaaaagtc
    50101 tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg aaggagtagc
    50161 tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag aactgatcca
    50221 gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat tggaagaata
    50281 tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc catttttttc
    50341 agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag ctgatgatga
    50401 gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg tcttttctat
    50461 tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg atttgtcgat
    50521 tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca tgttagacgg
    50581 attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct caaaaattta
    50641 tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa aatatcaggt
    50701 aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa ataaaataaa
    50761 aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc actataaaaa
    50821 agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat gcttcttgcg
    50881 ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa acaatcatgt
    50941 tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa aaagcagcag
    51001 aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca gataataatt
    51061 tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc atgaaagctc
    51121 aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat atgcaagact
    51181 atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg aaataaatca
    51241 agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag ccatcatggg
    51301 gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg cggctgctaa
    51361 aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat cacttataca
    51421 agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc atatgcaagt
    51481 gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt ttactgattt
    51541 agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac aacttctttt
    51601 ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag gagaactctc
    51661 tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag ttggatggag
    51721 aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt tgctttgcgt
    51781 gatgctttta tcggcatact gcccgcaaca atggcgggat cagtcgctgt aatgatcaat
    51841 gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag tttagcagca
    51901 gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt tgtctggaac
    51961 ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa tcttgcccga
    52021 gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt gattcaaggt
    52081 atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc aaatgtcgca
    52141 gatgcaatca atagttctga gattggtgca acgattgcag atggcacact gtcagttgga
    52201 ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac agttatgatc
    52261 atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat tacgatcaaa
    52321 atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt accagcgacg
    52381 atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc tgatgggcaa
    52441 ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt atctcaaggg
    52501 ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt tggtattcat
    52561 ggatcaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca actaatcaat
    52621 attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt gttagatgca
    52681 atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt tgcatggttt
    52741 ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc taaacgtgca
    52801 gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat caacgaacca
    52861 gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt cttgattgct
    52921 ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt cgctccggta
    52981 tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc aacaggagcg
    53041 gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt gatttgggca
    53101 ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca ataagatgaa
    53161 cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag gaggaactga
    53221 aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt tagaaggata
    53281 caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc tgttctttga
    53341 aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa tcaaagcaca
    53401 gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg tttaaaaaga
    53461 taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt ctttgatcgt
    53521 gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg tcacaggtct
    53581 ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag aagaagcaaa
    53641 ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc gtttttttac
    53701 gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa atcagtaact
    53761 agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa ccgggctcgc
    53821 ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc gacaacgaag
    53881 aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg tgaggttcat
    53941 tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat gcttttagga
    54001 acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat gatcgaagca
    54061 acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag taagctagaa
    54121 ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt tttgtaggaa
    54181 ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt gaggaaatgt
    54241 ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga gaacgttttc
    54301 gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat cggctaacaa
    54361 gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac ctaatggccg
    54421 gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc aatcagatca
    54481 tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat ctggcgccat
    54541 tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa atcagttatc
    54601 aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata aagcgtatgg
    54661 attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg gaaaaccaac
    54721 gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa aaaggcaatt
    54781 accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc gtttatcagc
    54841 ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat gctttttgca
    54901 gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa tattcatgcg
    54961 gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct gtaatgatca
    55021 caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat tatccattta
    55081 aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg aaatggaatg
    55141 aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa gtagaacaaa
    55201 caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta ttagggtatc
    55261 gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa tatgaagaaa
    55321 cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt tgataaaaaa
    55381 cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat ttgaaggaaa
    55441 atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt ttgttttagg
    55501 gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat tttttttaga
    55561 tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg gaaaggacct
    55621 ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt ggaaacgttt
    55681 tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa acaaacaagc
    55741 tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat cgcctcctaa
    55801 gcgataggtc gctggttcaa ttccagtcag gaacgtataa ataacccctg tatcctttgc
    55861 ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta tcatttttat
    55921 ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
    55981 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
    56041 taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca
    56101 aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg
    56161 cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttga
    56221 tttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg
    56281 tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat
    56341 ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt
    56401 cccatgttaa tcctagaagt ccagcaaatc gcattccagt ggcaccagca actaacacga
    56461 gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa
    56521 attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta
    56581 ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct ttcaacgacg
    56641 ctctaatatg gttattaaac ttaataacgg tagattttac atggtcttta gc
//
LOCUS       DAZKCC010000009        54734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000009 DAZKCC010000000
VERSION     DAZKCC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.009"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..1005
                     /locus_tag="ACG8VH_000563"
     CDS             46..1005
                     /locus_tag="ACG8VH_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9109147.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1175..1855
                     /locus_tag="ACG8VH_000564"
     CDS             1175..1855
                     /locus_tag="ACG8VH_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9109148.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            1945..2979
                     /locus_tag="ACG8VH_000565"
     CDS             1945..2979
                     /locus_tag="ACG8VH_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9109149.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2993..3328
                     /locus_tag="ACG8VH_000566"
     CDS             2993..3328
                     /locus_tag="ACG8VH_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109150.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3312..5792
                     /locus_tag="ACG8VH_000567"
     CDS             3312..5792
                     /locus_tag="ACG8VH_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109151.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            5848..7407
                     /locus_tag="ACG8VH_000568"
     CDS             5848..7407
                     /locus_tag="ACG8VH_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109152.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7404..8768
                     /locus_tag="ACG8VH_000569"
     CDS             7404..8768
                     /locus_tag="ACG8VH_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9109153.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            8939..10009
                     /locus_tag="ACG8VH_000570"
     CDS             8939..10009
                     /locus_tag="ACG8VH_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109154.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10024..10692
                     /locus_tag="ACG8VH_000571"
     CDS             10024..10692
                     /locus_tag="ACG8VH_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109155.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            10712..11290
                     /locus_tag="ACG8VH_000572"
     CDS             10712..11290
                     /locus_tag="ACG8VH_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9109156.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            11691..12602
                     /locus_tag="ACG8VH_000573"
     CDS             11691..12602
                     /locus_tag="ACG8VH_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9109157.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12980..13897)
                     /gene="rnhC"
                     /locus_tag="ACG8VH_000574"
     CDS             complement(12980..13897)
                     /gene="rnhC"
                     /locus_tag="ACG8VH_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP9109158.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14099..14536
                     /gene="zapA"
                     /locus_tag="ACG8VH_000575"
     CDS             14099..14536
                     /gene="zapA"
                     /locus_tag="ACG8VH_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9109159.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            14594..15142
                     /locus_tag="ACG8VH_000576"
     CDS             14594..15142
                     /locus_tag="ACG8VH_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFP9109160.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15257..17617
                     /locus_tag="ACG8VH_000577"
     CDS             15257..17617
                     /locus_tag="ACG8VH_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9109161.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            17704..18018
                     /gene="trxA"
                     /locus_tag="ACG8VH_000578"
     CDS             17704..18018
                     /gene="trxA"
                     /locus_tag="ACG8VH_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP9109162.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18200..19978
                     /gene="uvrC"
                     /locus_tag="ACG8VH_000579"
     CDS             18200..19978
                     /gene="uvrC"
                     /locus_tag="ACG8VH_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9109163.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20123..21022
                     /locus_tag="ACG8VH_000580"
     CDS             20123..21022
                     /locus_tag="ACG8VH_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109164.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21015..22277
                     /locus_tag="ACG8VH_000581"
     CDS             21015..22277
                     /locus_tag="ACG8VH_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109165.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22438..24336
                     /locus_tag="ACG8VH_000582"
     CDS             22438..24336
                     /locus_tag="ACG8VH_000582"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP9109166.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(24666..25385)
                     /locus_tag="ACG8VH_000583"
     CDS             complement(24666..25385)
                     /locus_tag="ACG8VH_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP9109167.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    25575..25622
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            25722..26054
                     /locus_tag="ACG8VH_000584"
     CDS             25722..26054
                     /locus_tag="ACG8VH_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109168.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26041..28044
                     /locus_tag="ACG8VH_000585"
     CDS             26041..28044
                     /locus_tag="ACG8VH_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP9109169.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28063..28533
                     /locus_tag="ACG8VH_000586"
     CDS             28063..28533
                     /locus_tag="ACG8VH_000586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP9109170.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28554..29138
                     /locus_tag="ACG8VH_000587"
     CDS             28554..29138
                     /locus_tag="ACG8VH_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFP9109171.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29153..30139
                     /locus_tag="ACG8VH_000588"
     CDS             29153..30139
                     /locus_tag="ACG8VH_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP9109172.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            30129..30440
                     /locus_tag="ACG8VH_000589"
     CDS             30129..30440
                     /locus_tag="ACG8VH_000589"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP9109173.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30462..32243
                     /locus_tag="ACG8VH_000590"
     CDS             30462..32243
                     /locus_tag="ACG8VH_000590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP9109174.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32236..33612
                     /locus_tag="ACG8VH_000591"
     CDS             32236..33612
                     /locus_tag="ACG8VH_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP9109175.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            33619..34254
                     /locus_tag="ACG8VH_000592"
     CDS             33619..34254
                     /locus_tag="ACG8VH_000592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP9109176.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34284..35639
                     /locus_tag="ACG8VH_000593"
     CDS             34284..35639
                     /locus_tag="ACG8VH_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9109177.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(35831..37225)
                     /locus_tag="ACG8VH_000594"
     CDS             complement(35831..37225)
                     /locus_tag="ACG8VH_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9109178.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37317..38288
                     /locus_tag="ACG8VH_000595"
     CDS             37317..38288
                     /locus_tag="ACG8VH_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP9109179.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38285..39067
                     /locus_tag="ACG8VH_000596"
     CDS             38285..39067
                     /locus_tag="ACG8VH_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFP9109180.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39236..39607)
                     /locus_tag="ACG8VH_000597"
     CDS             complement(39236..39607)
                     /locus_tag="ACG8VH_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9109181.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            39741..40184
                     /locus_tag="ACG8VH_000598"
     CDS             39741..40184
                     /locus_tag="ACG8VH_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9109182.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40227..41036)
                     /gene="yidA"
                     /locus_tag="ACG8VH_000599"
     CDS             complement(40227..41036)
                     /gene="yidA"
                     /locus_tag="ACG8VH_000599"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9109183.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41055..42422)
                     /locus_tag="ACG8VH_000600"
     CDS             complement(41055..42422)
                     /locus_tag="ACG8VH_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9109184.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42406..43236)
                     /locus_tag="ACG8VH_000601"
     CDS             complement(42406..43236)
                     /locus_tag="ACG8VH_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP9109185.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43399..43836
                     /locus_tag="ACG8VH_000602"
     CDS             43399..43836
                     /locus_tag="ACG8VH_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP9109186.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            43912..44532
                     /gene="rpoE"
                     /locus_tag="ACG8VH_000603"
     CDS             43912..44532
                     /gene="rpoE"
                     /locus_tag="ACG8VH_000603"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP9109187.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            44721..45227
                     /locus_tag="ACG8VH_000604"
     CDS             44721..45227
                     /locus_tag="ACG8VH_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9109188.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45244..46143
                     /locus_tag="ACG8VH_000605"
     CDS             45244..46143
                     /locus_tag="ACG8VH_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP9109189.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46230..47114
                     /locus_tag="ACG8VH_000606"
     CDS             46230..47114
                     /locus_tag="ACG8VH_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9109190.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47306..47377)
                     /locus_tag="ACG8VH_000607"
     tRNA            complement(47306..47377)
                     /locus_tag="ACG8VH_000607"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47342..47344),aa:Arg,seq:ccg)
     gene            47437..48771
                     /gene="rpoN"
                     /locus_tag="ACG8VH_000608"
     CDS             47437..48771
                     /gene="rpoN"
                     /locus_tag="ACG8VH_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9109191.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            48920..50011
                     /locus_tag="ACG8VH_000609"
     CDS             48920..50011
                     /locus_tag="ACG8VH_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP9109192.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            50052..51053
                     /gene="gap"
                     /locus_tag="ACG8VH_000610"
     CDS             50052..51053
                     /gene="gap"
                     /locus_tag="ACG8VH_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9109193.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51163..52353
                     /gene="pgk"
                     /locus_tag="ACG8VH_000611"
     CDS             51163..52353
                     /gene="pgk"
                     /locus_tag="ACG8VH_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9109194.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52457..53212
                     /gene="tpiA"
                     /locus_tag="ACG8VH_000612"
     CDS             52457..53212
                     /gene="tpiA"
                     /locus_tag="ACG8VH_000612"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9109195.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53328..54626
                     /gene="eno"
                     /locus_tag="ACG8VH_000613"
     CDS             53328..54626
                     /gene="eno"
                     /locus_tag="ACG8VH_000613"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9109196.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18193 a         9539 c        11542 g        15460 t
ORIGIN      
        1 cactcaaaat agttccgacg aaagaattat aaggagtgag tacaaatgac ctataaccat
       61 cttacaccaa ctgaactcgt aatgatagaa gcttatttca atcaatcaca acctgtttca
      121 aaagtagcca atctgttaca acgttctcga caaactattt ataaggtcta tcgttttctt
      181 aaatctggtg gaagcgcaat gagctactat aaacaatata agaaaaacaa acgtaactgc
      241 ggaagacatc cgattgtttt acctgatgag caacaggaat acattcaaaa aaaggttgtt
      301 caagggtggg ctcctgatgt ccttattggt cgagcagagt ttcctattgc ttgttcggta
      361 cgcacactgt atcgaatgtt taaagataaa acctttgaca ctcacgattt accaatgaag
      421 ggaaaaagaa aaccaaacgg tcataaagaa aaaagaggaa aacaagggtt tcgccggtca
      481 atccgagaca gaaagacaga ctatacccag tttgatcatg aatttggtca cctggaaggt
      541 gacactatcg tgggtggaaa acataaaagc gcggtcatca cattagttga acggctatct
      601 aaagtcatca ttaccttgaa acccgaaggc agacaggcta aagacattga aaataggctg
      661 aatcaatggt ttcgttgtgt cccaaaaaac ttatttaaat ccatcacatt tgattgtgga
      721 aaagaattct caaattggaa aaagattagt aataccaacg atatttcaat ttattttgcg
      781 gatccaggaa caccttctca acgaggattg aatgaacatt caaatggctt attacgtaaa
      841 gatggtttgt ataaagctat ggattttaat ggtgtatctg aaacctttat ccaatcggtg
      901 gcatcgaaac gaaatcatat tcctagaaaa tcattacact atcggacacc aatggaagta
      961 tttttgagtt acgtcacaaa agatgatttg tctaacttaa tttgacaaac gaaacttaat
     1021 aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt tttctctttt atacaaaaag
     1081 ggtcataatt tcaagtaagc ggatgaattc atgaggattt tttgcatact gtctctacta
     1141 tacttaggtc actaagaaaa aggagtgatt tattatgaaa aagaacatga ttgggacatt
     1201 attactttca acacttcttc taggcggctt ggcaacacca gcttttgctg agggtcaagc
     1261 gacttcaaaa ggagatatta cctttactga accaacaaat accgtggagc ctcttaaccc
     1321 tactgatcct tcaaaaccag tagagccagc agatccggaa aatccagcaa ctggccagac
     1381 aggtccttta acactggatg tagttcccga attgccattt ggtacacacg agattgaaag
     1441 cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat acaccttatc tccaagtatc
     1501 agatcgtcga ggtgtaggtg cagatggcca agcacaaggg tggaacgtga cagtttcggt
     1561 ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct gaattagatt ttggaacatc
     1621 cactgttaaa agtacgtcag ataacgaatc cactggacca actagtcaaa ctgtaactgg
     1681 gttgagtaaa gcaagtgcag cgacaccaat atttacagca gcaaaagatc aaggtctggg
     1741 aacatggctt tctgtttatg atccagcaaa tatcacactg aaagtgccga aagcagcagc
     1801 tggaacattc acggctgatt tgacttggaa cctcgtggct ggcccagtag catagagtat
     1861 gaaataaatt caacaaactg cggtgaaaat actataggtg aaggattttt taccgcagtt
     1921 gttgttgaaa ggatgtggtg gacaatgaat caaagtaaac gactgtttgt ttcgtttttt
     1981 tctattttaa gtctattttt catttttccg tcaatcagcc aagcaaagga ttcagcagga
     2041 gactttggaa tcaaaccggt atttccagag aaccaaatag ataaagcaat cggttatttt
     2101 gatttattag tggcaccgga acaaaatcag acactagaag taattatcag caattcttca
     2161 gatgaagaac gaacatttga ggtctctgtc aatccggcgg taacgagtga tggtggaacg
     2221 atcgattata gtcaaaaaaa cccaacgtta gatgagacat tgccgtttga tgtccgtgac
     2281 gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac atgcagaaac aactgtacct
     2341 atagaagtga aaatcccagc aaaaagcttt aagggacgtg tattagcggg tattcatgtt
     2401 tcacctaaag aagaagctga aacagaaaat gctacagaag gagcacaaat caaaaatcgt
     2461 attgcttata atctcgcagt cgttttacaa gaaagtcaag agacgataga accggatctg
     2521 aaacttttat caggagattt ggacgaggtc aacgcaaagc caactgttca gcttcgtttc
     2581 caaaatcctc agcctaggat tatcagcaat ttgatattta cgagcaagat cttttatgaa
     2641 aatcagcttt atattgaaaa tacgtctaat gcatttttag ttgcaccaaa ttcgaatttt
     2701 cacttgaatc ttgatttagc tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc
     2761 atagcaaaat caggcgacag taatgaatgg cgatttacac aaaactttac gatcaaaaaa
     2821 gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag tacaagaaca atctttccca
     2881 tggctttatg caggattggt tgctgcagtt ctagtgttat ggttgatttt gctgtttttg
     2941 tgggtccaca agaaaaagaa aaaaggagaa acaacatgat gaggtgattt cgttgaaaac
     3001 atcgatctta ttgggaggga ttattgcaag tttgctgttc ttcagctggc aagagccagt
     3061 ccaagcagat accaaaagtt caggtacgat tacattttat gatctaagtc caattccgca
     3121 tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca cagctaccga tttataaaga
     3181 atctaatcaa catggcgtat tcccggagac gaatgaacaa aagatccgtt ttctttattt
     3241 gatcattggt atagaattat ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa
     3301 ggaggatgaa catgttgcta caaaataaat tttcattttt tcgctccttc ttttttactt
     3361 cgctttttgt tttaagtata tttatctttt caccttcggc agaagctgtt ggagcaacgc
     3421 ctcctgccat ttcttatgat ccatacacgt atgaaaatat cgtacctcca aatggtggac
     3481 gtagtgcagt caatggcggg aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat
     3541 tgactgctac ttatttgaac gatggttcaa atgatccttg gaaaggtggc acggtccgtt
     3601 ttcattggga aagccatgtg aaactaacaa atgtacttga tttggtgaat atcactgcac
     3661 aaggatttgc atttgtggcg atcatcaatt tgccggaaca tgtatcaagc agtgatgtat
     3721 taaaagcaat tgactggaac aacgcctact tgtatattgc cggtgccccc atcaaggtta
     3781 ctagcggaag tttattatca gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg
     3841 atacaaatac tctattgaca gatattattg ctattattac gcgaggcgaa ttcaacgtgg
     3901 acgatatccc gcttcaattt gatgttacag ctaatgtcgc gaagatgaca gaaaacggag
     3961 ataaatatga tacgagtcca aacaaaattt tgaccaaggg taaattacaa ccttctatgt
     4021 ctaaaaaggc cgttttttct gttgattttt atgattcgaa taatattatc caagatcgta
     4081 tgttcaattc gattttagct ccagctgcag gtagatttta tcctaaatta actggcggaa
     4141 acttgaatcc aggagcatat gcattttctg caagtgcaga gataaatacg tggaatagtt
     4201 acatctcacc aagtgattca acaggaaaat ataatacatt tgctgatacc acagaatacg
     4261 tggatggaaa taaccagaac cttccaggct ctaccaatag tcggacattg acgatggggc
     4321 tgactgaagg acgttttgat cagttgccat ttagtcgctt tgatcgcgta gtcaactatt
     4381 ttacaggaaa aaacgttaca caaggagcaa tactgactca tactccagca agcacaagtg
     4441 gattgaatca gacagccaat atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt
     4501 ctcctgttgc tttaaaggta ttggagcagt acaagctaga cggaacggtt ttaccaacaa
     4561 acttaacagc cgatagtcag tggggaaagc tcctttcttc tgcatcgtac caagtcacgg
     4621 ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag
     4681 gaacccaaaa attagcgaca ggatggacag atcaactttt ccaaacgatc caatcaaata
     4741 acgggggtgt aaatacagct acttctacac tacctgcatt ggcttcaggg caatattatt
     4801 ttgattatcg cttggtggac gatgtactta ctaacatgta tccgtctttt gcgtacaaat
     4861 ggcagtctga acaagctgga atcaccactc ttcctcaaat aacagtggct aattttccat
     4921 ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca acctgagaca ggagatcccc
     4981 tgtctgctct atcaggagat acgattcaag aaaatgtgac attcacgtta acaaaatctg
     5041 gggatgcgat aaccgacaaa aaaatcacga tctcacttcc tgaaaatact acttatatca
     5101 atggttcctt aaaattaaat ggtacaacta tcgcagatac cggcattcaa caaggagttt
     5161 ctgtcccagc agatctattg agcaaaatag gagataccat tcatcttact tacaactatc
     5221 aattaaatac tgtagatact tctttacaat ccgtcagcat actgacaaag gcagctgtac
     5281 tttccagcaa tattacatta gcagatggtg ctaagcttcc taacccagtt gtccaaacat
     5341 ctgctaaaac aattcttgtc cccaaacaag aattaacatt agttaatgta ccagatgatt
     5401 ttacatttgg taacgatctc cccaaaccgt tgaagacaag ttattatgaa tcaaaaggcg
     5461 atttttcttt tgatgtgcgg gatactcggc ttccaagcac gtctccttgg caactgacag
     5521 gtacattgac ttctttattt aagaatgatc aaggtcaaga attaagcgga acaaaattat
     5581 acttcaatca ttctggatca aagcaattga ttcagcaagg tcaaaacacg ttgatttatg
     5641 agagtgatgg aacagctaaa ggagaggttc tcgttgactt tcccgatacc gatggtttac
     5701 tcttagaggt aaatagcagt acgaatgctc aacctggcgc aacgtatcaa ggcatggtca
     5761 catgggaact gacagctggg ccaaccagct aagtgattag aatttgtatg gatctgtgat
     5821 aactgtcaca gatcctcttt ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa
     5881 atgcttgaag gagtggactt ggaacttttc agtttgcttg aaaaaaatga acaacggcaa
     5941 atcaaacttt tccaactttt attagaggaa aaaggagagg tttccgttaa taacgtactt
     6001 acccgcttat cagttgatcg ggctaccttg aaagaagatc tgttttattt acaagctaac
     6061 ttggaaaata tcaaacaatt ggaaattgtt atccaagaag ggaatatttt attaatcaga
     6121 catggaaata tagcagcagt cgatgtttat tatcattgtt acttgcgcta ttctacaaaa
     6181 tatcagatac ttctttatct gttggaaaat ggctcatttg atcggcagaa acttgtcatg
     6241 gaacttaata tcagttcggc tactttgtct cgcagagtga aggaattaaa tgattcttta
     6301 aatgagtttc atctgaaaat aaaaagcggg cggctgaccg gtccagaaag tcaaattagt
     6361 tatttttatt tccaattatt ttggtttgga cgtccctatc ttgaaaacaa aaaagaatac
     6421 agtgatttat caggaaacac ttttttagat ctgctgcaaa aagagtttag ttttgctatt
     6481 acagaagaag gacgattgaa atttgaaata tggctttcca ttatgaagaa gcgaatgcgc
     6541 tgtacgaaaa aatccaaacc agattatagc aatcttcctg ttggattgtt tcaaacgaca
     6601 caatttttat tggacctcca tacatttttg acccgttact tctttcaata tgcctttcct
     6661 tggaatcaaa atgaaacgtt ggttttttat cttttcatga tcagtaactt tacattagat
     6721 acacataact cgcatgtcca ggagtttttg ataagcgaaa aatacaaaga cgacgtagta
     6781 gctcaattga atcgaacgtt taaacaatcg atagaagaat atttctcttt ttatgttttt
     6841 ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc aaaatcacgc tcattttgcc
     6901 tattttagag gatggatgaa tatttttggc caagaaggat taaaacagcg gttaactgaa
     6961 gtagaagata aacaattcct ggttttatgt gaaagattgg tcagacaaac atttgctatt
     7021 gttgatttta ctgaagtaga cgaggaaaat gcccgctttg aattatttgc tcgctatgca
     7081 tctattttac ggacgctgat caagcagtta gaattacgtc tgcaaattgc ttatgatctt
     7141 ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca tccaggaaca attgctgaat
     7201 acgatcaaat atgatttagt cgaatacgag aaagaaaagc agtatgatat cattttgact
     7261 agccaattga aagagtaccc gtcacagaag tccgctaaag tttttgtatt cactagcaac
     7321 gaatataatt atgatttccc ctatttgaac caatttttaa aagaatgtta tttagaaaaa
     7381 ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat tggtgcttcc catgaaggca
     7441 tcacagctgc cctaaatcta agaaaacttc aaccgaaagc agaagtttta ctgatcgatg
     7501 aagatcataa agatggacta ggctatgttt caaatggaat caacctttat ttaaaaggaa
     7561 agatacgtag tttagctgaa gttgcccata acatgcggac tcttcaagaa agtggggcaa
     7621 aactgatcac cgaatggaga gtaactgaac ttgatccaga caaacatcag ctcatcctcg
     7681 catccaaaga aggaaaaaac gagaccataa cctatgataa attaatcgta gcgacaggtt
     7741 cctctcctgt caccttgtat aaacaaattg aagctgaaaa tgtctacacc tataaaaatc
     7801 tagtgcaatc taagcaagta cttgccgctt taaaagaagc aaaagaagta gtgatcttcg
     7861 gtgctggtta cattggattg gaattagcag atgctttgag aaataaagga tacattattc
     7921 atctagttga ttatatgccg aatgttttat caagatattt cgataaggat atgatcaaca
     7981 gttttcaaaa tcagctgcaa actaagcaaa taaattttta tccaaatgaa ttcttgatcg
     8041 attggaaaaa atcagaagag aaagtagtat ccgtacaact tctgtctcaa acaataaagg
     8101 cagatatggt cattttttct gcccaaactc gtcctaatac gacattgtta aaagaaaaag
     8161 tggctttgta tgaggatgag actgtgatag tcaatgaata tctgcagaca agtgatccgg
     8221 acatctatgc gattggagat atcgtcccag tcagttttga caaaaataaa cggcatctct
     8281 ttttaccttt agttactcga gctgtacata tggcaagagc agtagcgcta actttgagtg
     8341 gtcagccaac cgcttacgac ctcagacaaa agataacagc agcagtcatt atagactact
     8401 ttttaggaac tgtagggctg acagaagatg aagcgccatt cttggaacaa aataccggtt
     8461 cgtgttcagg agaatttgat ctatttccac aatacgacga agaaaacaaa acagtaaatg
     8521 caaagctgat ctaccatccg gatacattag aaataattgg cggacagctg ataagccaag
     8581 aattcttatt atcagatttg aacctgcttg cggatatcgt gaaacacaaa actacgattc
     8641 cacagctagc agttgaggat tttggatttc tgtctgaata cacctctcgt tttcactacc
     8701 tcaatgagtt agcttttaaa gtactagcta agagagcaaa tagcaataga gtagataaaa
     8761 gactgtgaga aatatcacgg tcttttattg tgtggggata taataaatat taatcaatcg
     8821 agaataaaaa attcatataa tgaaatagcg gaagaatttt ttgagcataa tattcgccaa
     8881 aaatttatca agtgataaaa ttgttgtata aaatgattat ttcaggagga ggagttaaat
     8941 gtttttagca ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag ttgcaggggt
     9001 gatgtttttg atcgcctacc tggtattttt tttgactggg ttagcttatg gcttagctca
     9061 agataatcga acagcagtcg acaagtggga ggcagattcg attgtgcttt cgaaagatgc
     9121 aaattcaaat ctagggatgt ccatgattac taagaagata gcagaagaag tcgaaggtgg
     9181 gaaagtggcg tatcttgccc aaacacctgg agtcgtgact agtaaagaca gtacagaaga
     9241 aggaaaaatc aatgttagct tcttcggtat cgataaaaat caattcatca tgcccaatct
     9301 tgttgaagga aaagcttttg ataacgatga tgaggcagta ggcgatatta gtttgaaaga
     9361 agaatatggt ctagccatag gagatacagt taagttatct ggtagtgaca agacgtttaa
     9421 actaactggc tttacggatc atgccaaatt caatgtctca ccagttctct atacaaccat
     9481 caatgcttac caagaaatcc gcttcgaaaa agaagataca agtgaaaatg cacggattaa
     9541 cgcaatagtt gtcagaggta aaatcagtaa tttacctgaa gatttagaac agatcaaaat
     9601 ttctaaattc atcaatgaat tacctggata taatgcgcaa gtattgactt ttggatttat
     9661 gatcggattt ttgatcgtta ttgcggctat tgtgatcggg attttcattt atgtactgac
     9721 gatgcaaaag attaatatct ttggtgtaat gaaagcacaa gggatcacag gaggatttat
     9781 tgcgcgttct gtagtggcac aaacattcat tttatcattt gttggtattc ttcttggtct
     9841 attaggaaca gtgggaacat cccttgttct acctgatgcg gttccattcc aaagcaattg
     9901 gctgtttttt ggcgtcatta gcttgttgat gttagtagta gctgtgttag gcgcactatt
     9961 ttctgttcgg acaatcgtaa aaatcgatcc gttgaaggca attggataag aaggaggaag
    10021 aacatgaaag caatagaatt taaagaagta gaaaaaagtt tccaagatgg cgaccaagta
    10081 gttaaagcat tgaaagaaac aaccatgagt atcgataaag gggaatttgt tgcaatcatc
    10141 ggtccttctg gttcaggtaa aagtactttt ttgactttgg caggcggatt gcagacgcct
    10201 agcagtggct ctgtatttat caatgatcaa gaattcagta atcaaaaaga aagcgaacgc
    10261 gttaaactac gttttaaaga aattggcttt attttgcaag cttccaatct ggttcctttt
    10321 ctgacagtaa aaaagcagtt attattagta gataagatta aacatgaaca tcgtcaaaaa
    10381 gaagcaaacg aattatttga gcagcttggc gtggataagt tgatcaacaa gtatccagaa
    10441 gaactatctg gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta caatgatccg
    10501 tccattattc tagcagatga accaactgct agtttggatt cagaaaaggc acgagaagta
    10561 gtcgatatct tggcaaaaga atcaaaagac aaaaataaag caacgatcat ggttactcac
    10621 gatacacggc tgattgacaa atgcgataga atatttatga tggaagatgg tgtattgaaa
    10681 gaaaaagaat aaccaaaaag caggtggaaa aatggtccat cgatacaaca aagatacaca
    10741 gcaaaaaatt ttgaaagaag cgactgactt gtttatgtcc aagggatatc tcggcacttc
    10801 aacaagagaa atcgcacaaa aagttgggat cacccagccc aatctctatc attatttcgg
    10861 tgataaagaa aaactatata cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc
    10921 tttgagagaa atcgttcaaa caagcgaaac gggatttcaa gtaaccttga caaaaatggc
    10981 gcagtttttg attgagacac atctggtcga tttgtttctg atgcttcacg atcttgaaag
    11041 caatctttca aaggaaacaa gagatcatct tttctttcta tggaagaaaa attatcgtga
    11101 accttttgaa gaaatctttt cagcaaacca atctgtgcta agaaatggga tttcaaaaga
    11161 aatcgcagca aggcattttt tcttagtgtt agcaccttac atcactcggt cgaaaagacc
    11221 tgtggaagaa ccgctaaatg tggcgcaatt gatcgattta tatttgaatg gtgtattagg
    11281 agaagcgtaa agctgttata tagggagaaa atagttaggt gaagaagcaa gatatgacga
    11341 agatcgttat atcttgcttc tttatatagg tagtttttca taaatacaaa tttgttattc
    11401 tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga agtgttttaa
    11461 tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt gggtagatga
    11521 atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa aaatcatttt
    11581 agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc ttacttaagc
    11641 tgattttaag ttttggccct tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa
    11701 taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa atagatgttt
    11761 ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg gttacaaaga
    11821 caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga aaaaatgacc
    11881 gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac ggcggagagc
    11941 tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat gatggctata
    12001 ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa acaaaaccgg
    12061 aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat gatactgggg
    12121 cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca gccataaaag
    12181 taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact acagatgaat
    12241 tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat gtctttatca
    12301 gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg acatattatt
    12361 actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact gatctgcctc
    12421 tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat cttcaacctt
    12481 ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa ttagtaacag
    12541 acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact tattttcagt
    12601 aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg ccgggcttat
    12661 tatcgcagat gcaagctttt aaacaccgtt taaggaagag gtcgtgagcc tgccgttcaa
    12721 ctctgagtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga ctattgtgac
    12781 ttgtgaccga ggagttggca tgagaacacc gtttaagaaa aaggttgtga aatcaacgtt
    12841 ttgttctgag tattagagaa acaaacggaa aaatagttcc tcatattttg ccgtttgttg
    12901 agctaatacc gaggatcaga tgattgaaca ccgtttattc ggaacaaggg actgtgacat
    12961 gacttttgtc acagttccat tattttttta gtagcttcaa agctttttct gtattggcaa
    13021 aatgaagttt ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg
    13081 ctacttggtc agatttcgtt ccagctcctg acggaagggt gattccagct tcagctgatg
    13141 ctttgtctaa ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta
    13201 aatgatattg ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta
    13261 aacgcgcata ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg
    13321 gtttttctgg ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt
    13381 tcatccgaac ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa
    13441 gcagtttata ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt
    13501 ttaactcttt tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca
    13561 cataagcagc acatactgtt agcggaccga aatagctgcc atttccgacc tcatcactac
    13621 ctaaaacgga taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg
    13681 cttgtttatc ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa
    13741 acatcacttt tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc
    13801 tagtataagg aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa
    13861 gttggtcgtt tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca
    13921 taaaactata ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt
    13981 ttttaaagtt tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa
    14041 gaaccaactt catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat
    14101 ggcgcacgaa aaaacacgct acaaagcggt gattgccaac caaacatata ctattatcgg
    14161 acgggaaacc aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga
    14221 attgaaacaa ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa
    14281 tgcgctttcg gatcaattga agaaacaaga aagaatattg gagctggaag aagaaacagc
    14341 agaattaaag aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga
    14401 agcaatcgag aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat
    14461 taaaaatcat gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca
    14521 aaaatcttct aattaagatc cttttatcaa aataagaaaa atttaaaaaa gttagaagag
    14581 aaaggaacgt agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc
    14641 tggagcacgt cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt
    14701 tattgctgcc cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata
    14761 cccagctgtc accccaacat caaagttagc attttttgat caggtatttg cctttcacct
    14821 agatgaagct ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct
    14881 tacccgattt gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt
    14941 agactggatt gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat
    15001 tttgcagtta cttgcctttg ttccattaga tgtggtccaa aatcaattcg gcgagcatag
    15061 tttcgctcgt tttatcgttg agagaacgcc atttcttacc aacaagatgt atgagctttg
    15121 ggtaacgaat attttgaatt aaggaaaata gtgctggaaa atatttcgtt ttttgtacag
    15181 agtagtttat ttaagaaggc tgcgtagcgt atgtccggca cagctctttt ttcatagtga
    15241 aagataaggt gataaaatga ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa
    15301 acagatggtc agacaatttg tagtgactgc acaaggaaaa gaagaactgg cagaattggt
    15361 accagtgagt gagaaacaaa cgatcacaaa ctggcttcaa gaaactgagg atggattgaa
    15421 agtgcaacgt ctgcgaggcg ggatccctat acctaaaatt gaaaatatcc gtccccatat
    15481 gaaacggatt gagatcggag cagacttgaa tggagtggag cttgcacaag tttccagagt
    15541 attgtctacg acaagcgaat tgaatcgttt cattgatgat ctttctgaca gtgaaatcga
    15601 atttgcacgt ttgtatatgt gggcagatca gttagtcacg atccctgttt tgagccgccg
    15661 tttaaaggaa gcaatcgatg aagacggacg cgtgactgat gacgcttctc ctgagttaaa
    15721 gagcataaga cagaacattc ggcgaagtga acaagctgtt cgtgaacagt tagatgggat
    15781 cgtccgtggg aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc gaaatgaccg
    15841 ctacgttatc ccagtcaaac aagaataccg cggtgtattt ggcggtgtag tccacgatca
    15901 aagtgcttca ggtcagactt tatttattga accaaaacaa gtggtggatt tgaataatcg
    15961 tttgcgtcaa taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc
    16021 tgctgaactt gttccccatc gtcaagaaat catccacaat acttatgtga ttggtaaaat
    16081 ggatctgatg aatgcaaaag cacgatttgg taaagaagta aaagccatcg tgccgggaat
    16141 cagtgaagac aatcatgtcg tcttgaaaca agcaagacat ccactgatcg atcaggaaaa
    16201 agtagtttct aatgacatca caatcggaaa agactatcaa gccatcgtca ttacaggacc
    16261 taacacaggt gggaaaacga tcacgttgaa gactttaggt ctgttacaac tgatggggca
    16321 agcaggatta ccgattcctg caggtgaaga aagtcaaatc ggtatttttg aggaagtatt
    16381 tgcggatata ggagacgaac agtcgattga gcaaagtcta tcgacctttt cttctcatat
    16441 gacgaacact gtagatattt tatcaaaagt caatgaaaaa agtctggtat tatttgacga
    16501 gctcggtgca ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga
    16561 tcttggtaaa aagtctgctt atgtgatggc aacaacgcat tatcctgaat tgaaagttta
    16621 tggatataat cgggcaaata cgattaatgc cagcatggag tttgatgtgg atacattaag
    16681 tccaacgtat cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg aaatctctag
    16741 ccgattaggt cttgatacag aagttattga tgaagcgaag cagttgatga atgatgagag
    16801 ccaagattta aacgaaatga tcacagattt agagaatcgc cggaaaatgg cagagaccga
    16861 atatctggaa atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg atttgaaaga
    16921 agcttacagc tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc
    16981 aaatgaagtc gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg atattcgtaa
    17041 aatgcaacag cagattgggc aaggaaatgt gaaagagcat cagttgatcg atgcaaagac
    17101 ccaattagca aatctacatc aagaagaaac tttgaagaaa aataaagtat tgaagaaggc
    17161 gaaagaacaa aaaacgttga aaccgggaga cgaagtgttg gttacgactt acggccaaag
    17221 agggacgctt ttacgtaaaa acgggaatca gtggcaagta gaaatcggta tattaaaaat
    17281 gaatgtgtcc gaagacgagc tgacgcctgt tgctcctcaa aaagagccga cacaacgagt
    17341 tatccatgct gtacgatctg aatctagtag tcacgtgccg aatcaattgg accttcgagg
    17401 aaaacgatac gaagaagcgc taagtgaggt agatcaatat ttggattctg ccattctagc
    17461 tggttatcct caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat
    17521 tactgattat cttaaaaacc atcggagcgt caaaagcttt gagtttgcac cggcaaacca
    17581 aggcggaaat ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg taagcagatg
    17641 gttcgctatc caagtataga aatgctatac tagtcataga ttttaaaaat ggaggtcttt
    17701 gatatgtcac aagtaattac agataagaca tttaatgaag aaacagataa aggacttgta
    17761 ttgattgatt tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc gattttggat
    17821 cagttggaac aagaatatga tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa
    17881 aatccagaaa ctcctcaaca attcggtatc atgagcattc caacattaat gttgaaaaaa
    17941 gatggtcaag tagtagaaaa agctgttggt gttcattcaa aagaacaact acgtcaaatg
    18001 atcgatcaat atttataaaa acttacagca aaaataagct gagggtggga ctgaatccag
    18061 ttccgtcctc ggtttttttg cggttgagtt aaggttattc aaattaatat tttttgagta
    18121 taattacgtt gtcaggctgg attgtttttt tgacagacat gctataataa ctttaattga
    18181 atatgaaggg gagtaccaga tgaacgaacg aatcaaaaac aaattagctt tacttcctga
    18241 tcagcccggc tgttacttga tgaaagataa aaatggaacg atcatttatg tcggcaaagc
    18301 caaaatatta aaaaatcgtg tccgctcata ttttactggt agccataata cgaaaacgga
    18361 acggcttgtt agtgaaatcg aagactttga atacatcgtt acagaatcga atatcgaagc
    18421 attgctctta gagatcaatc tgataaagaa aaatgatcct aaatacaaca ttatgttaaa
    18481 ggatgataaa acctatcctt tccttaagat aacgaatgaa aaatatcccc gtttggtgat
    18541 tactcgtaag gtactaaaag acaaagcact gtattttgga ccatatcccg atgtcggtgc
    18601 ggcgaatgag acaaagaaga tcctcgatcg attatttcct ctgagaaaat gcaaacctag
    18661 tcagacgaag gagccttgtc tttactatca tttgggacaa tgcctgtgtc cttattattt
    18721 cgacgtagat ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaact ttttaaacgg
    18781 aggacacgaa aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat
    18841 ggagtttgaa aaagctgcag aataccgaga ccagatccgt gccattgaga caatcatgac
    18901 ttgccaaaaa atgacaaata ctgatttact ggatcgagat gtatttggtt atgcgattga
    18961 taaaggatgg atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga
    19021 tgtttcaatg tttcctttct ataacgaagc agaagaagat ttcttgactt acctcggtca
    19081 gttttatcag gaaaatgagc atttcattcc taaagaagtc ttgattcctg atacgatcga
    19141 caagcccagt gtagaagctt tgttagctac gaaagtgctg caaccaaaac gaggagaaaa
    19201 gaaaaaatta gttgaattgg cgagtaagaa tgcctcagtt gctttgaatg aacggtttga
    19261 tctgattgcc agaaaacagg aacggacgat cggagcagtt gaacgtttag gaaacgcgat
    19321 gaatatcccg accccaatac gtatcgagtc ttttgataac tctaatatca tggggacgaa
    19381 tcctgtggct gcgatggtcg tattcattga tggaaaaccg gcaagaaaag aatatcggaa
    19441 atataaaata aaaacagtca acggaccgga tgattatgca tcaatgagag aagtcatcta
    19501 tcgcagatat tcgcgagtga ttcgcgaagg gttgccttta cctgatttga tcttgatcga
    19561 tggaggaaaa ggacaagtag atgtggcaaa agatgtctta gaaaatcaat taggaatcga
    19621 tattcctatt gctggcatgg taaaaaacga taaacataaa acaaatgagt tgctattcgg
    19681 tacagatttg gctgtagtac cgcttgagcg aaattcacca gagtttttct tactccagcg
    19741 aatccaagat gaagtccatc gattcgcaat cactttccat cggcagctac gcagtaaaaa
    19801 cagttttgct tcccgattag atgaaattga agggttagga ccaaaacgaa aaaaaatgct
    19861 gcttaaagag ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag aattacaaga
    19921 aatcggactg ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc aggaatgagg
    19981 aaagggcact ttacaaagaa agtaaagtgt cctatcattt tatgtacaac aaaaagagcg
    20041 tttacgtttt gttggtaaat aataaatcgc ttacgtctgg tgtctaagga tatatgtgat
    20101 ttcttgaaga aggagtgaag aaatgttaga agtcaaggat cttgttaaaa cttttgggaa
    20161 ctacacagct gtcgatcatg tctctttcca aatccccgat ggaaaaatca tggggctaat
    20221 tggccaaaac ggggctggga agacaacgac ttttcgtttg atcttggatt ttttgacaca
    20281 agaccaagga gaagtgttat ggaatggcca tcatcttggg gagaaagatt atgatgtcat
    20341 cggttatctt ccggaggaac ggggacttta tccgaaagta acgattcaag accaactgct
    20401 ttattttgca gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa tcgacttttg
    20461 gatggaaaaa tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg
    20521 aaatcagcaa aaagtccagc tgatcgccac gttaatccat gaacctaaat tgatcatttt
    20581 ggatgagcca ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag atggaatatt
    20641 ggaattgaaa aaacatggtt catgtgtcat tttttctagc cacaatatgg ataatgtaga
    20701 aaaaatctgc gatcatttga tcatgttgcg aaacggaaaa atggttttga atggaaaagt
    20761 acatgagatt cgtgaatcat ttggccgtac gaagttattc ttggaatctg gtttaagcca
    20821 acaggaagta gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt
    20881 agagattacc ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt
    20941 cggctacatt cctatgttta accagcaacc gccaactttg gaagagattt tcaaattgaa
    21001 agcaggtgaa atcgatgcgt aagttttgga ttattaccaa agatgtctat ttaaaaaatg
    21061 ttaagtcgat ttctttcttg atcatgatat tagtcccttt tatcatgctg ggtatcattt
    21121 atgtagcagg aaactttgct caacaaaata gtgatgtcaa taaaatcggc gtattggctg
    21181 ataatgagca aatcagtcag caaatgagcc aagtcaaaac agatgatttt gagttcaaag
    21241 cagttgattc tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt
    21301 tagtagtctc aactgagaat gataagataa atgggatgct ttatagcgaa aattcattag
    21361 gacaaagtac acaactactg atacaacaac aactgactgg cttgcaatcg atgcttcgag
    21421 cgagtcaatt aggcatttca ccagaagaag tagctagctt gagccaacaa gcaagtttga
    21481 ctcgacaaaa agtcaatttt gattcaaaag gaaaaatgac agtcggagaa gataactcga
    21541 cagtccaata cgtcgtaagt tatgtagcaa cgattgtctt gttcattatt attttgacgt
    21601 atgcgcagat cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc atggaagtga
    21661 tcctgtccag tacgactgca caaaagcatt tttacggaaa attaacaggt gttttacttg
    21721 ttgcattaac gcaaatggca ttatacggta ttatttttgg tataggatac agtcaattca
    21781 aagatatgga aatggtcaaa aatatattag acggtatctc attggataac ttgattggac
    21841 cattcttgtg gtattctctt ctattcatga tctttggcat tttgattttt tctgttttag
    21901 cagctttatg tggctctttg gtcaacaaag cagaagatac agcaaaagca attttgccag
    21961 tgacttatct atctctaggc ggatacatgc ttggattgat actaggagct tcagatccaa
    22021 acaatatcgt tattcggatc acttcgtata ttccattctt atcttcatat attatgccga
    22081 ttcgcttagc aaatgagaca gtcggtatgg gtggagcatt gatttccttg ttggtacttg
    22141 tagccgtaac attcttattg atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg
    22201 tttatagcga aggcggatta tggagttcat tgaaacaatc gatttcaatt atgaggaatg
    22261 aacgaaaaaa agcataaaac aatcctaaaa tacagaagga agctggacat ctattgttcc
    22321 cagcttcttt ttgtatagac ctattttatt tattttacta tattggatat atattcattg
    22381 aaaatagtgt ataacaactt tataatattg agtataccaa tttttggagg cgttttaatg
    22441 gaccgttatt tagatttact ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa
    22501 atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagtgat
    22561 atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa
    22621 gaaaagatca aagacctttt ttctgaacaa atgacggaaa cggatatcag ccaattcact
    22681 atttttacgg cttatccaga atatgcatta acgactgatt ggtacaaaga acaagataaa
    22741 tcgcagctga tccatcattt gatcgatctt ttacggttta cgacagtcaa atatacccgg
    22801 tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat
    22861 atcgatgacc gtatcaatgg aaaaaaagac tatgtaaaaa aaatcattga acaacttgtt
    22921 ttgatgaagg aagaagaaaa atttttaacg aaacttgcac agacgatcca aaaatctgtc
    22981 atcgatcatt tgcatatcgt tggagatatt tttgaccgag gaactcaagc agccaaagtg
    23041 atggatcgaa tcatggattt ttcttctgta gatatccaat ggggaaatca tgatattttg
    23101 tggattggtg cgtattgcgg ctctgaggca tgtttactga cacttttacg gattgcagca
    23161 cgctataatt atttatttga attagagaaa gaatatgggt tgaatttacg tccattgttt
    23221 tcttttgctc atcaaactta tcagaagaat ccagtatttg cgcctaaaaa cagtaaaaat
    23281 ctatccgaaa gagagcaaga agaattagaa aaaatccatc aggcactttc tatccttcaa
    23341 ttcaaatcag aacatcagtt attggatcgt cgacctgaat ttaaaatgga cgatcgtaaa
    23401 ttgctggaaa aaatcgacta tgaagcatca acgattgatt tagaaggtca gctctatgag
    23461 ttgaaaggaa cttgttttca aacgatccag ccagatgatc caaaaaaatt cttggaagag
    23521 gaacaaaaag tgattgatag tttgatgtat tcattccagc actctttgag aatgcaaaaa
    23581 catatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtgctg
    23641 tttcatggct gtatacctgt agacgagaag ggacagctct tagcatttga attagaccaa
    23701 tcttatcgcg gaaaagaact gctgggcttt tttgaaaaac agataactga aagcgcaaaa
    23761 gatctgactg caaaagaaga tttagcaacc gatttgattt ggtatgcgtg gagtgggcca
    23821 ttttcgcctt tattcggcaa gagtaaaatg gcgacttttg aacgttattt tctcacagag
    23881 cctcatacgc acgtcgagaa aagcaatcct tattatcact tgcgagatga agagtggttc
    23941 agtgaaaaaa ttttagcaga attcgacaca aaagaagaag ggtcagcaat cattaatggg
    24001 cacacgccag taaaagtaaa aaaaggggag tcaccgatca aagcaaatgg aaaagtattt
    24061 gtcattgacg gaggtttatc caaagcttat caaaaaacga caggaattgc cggttattca
    24121 ctattatgca attcttatgg tttccaaatc gtgacacatt atccgttttt gcctattgaa
    24181 caacaatttg caaaaaaatc cgaccaaaca aatgtgaaaa aagtaatcga acagcaacta
    24241 ccacgcaaac tcaatcgtga tacaacaaaa ggtatggaac tgcagcagca gattacccag
    24301 ctcaaaaggc tgttggacta tcgaaaggac gattgagaag aattccagtg aacattgttc
    24361 catagactgg agactgagac aaaagtcgtg tcgcagtctc tttttccgga taaacggtgt
    24421 tcttctgcca actcctcggt cacaagtcac actattcaac aaacatgaaa agctgttttt
    24481 tgaaattgct ccttgtgact cagagttgaa cggcaggctc acgacctctt ggtataaacg
    24541 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt gaaaagccat
    24601 tttcacaaaa tttttcttaa tactcgaaac taaacagctt tttcacaacc ccctcagaaa
    24661 gtctattaaa attcattcac aaagaaatcg aataagcgct gttcagatgg atgtacacgg
    24721 tgggtaagtt ccggatgcca ttgaacaccg aggattttat gcatatcatc tttgctttga
    24781 accgcttcaa cgagtccatc gttagaaaaa gcgatggctt ttaataaagg tgaaagatct
    24841 ttgactgcct gatgatggta agaattcaca aaatatggat cggggagaag gttgcttaac
    24901 aaactgtctg aaacaatctt gacttcgtga gtcgtaaatt gcggggcagt gggctgttgt
    24961 tcatgtttta ctgaccactt tggatataat gaaagatctt gataaagtgt accttccaaa
    25021 acgacattca atagttggag tccccggcag actgcaaaaa ttggttttct ttgtttcaat
    25081 gcttccaaga tcagtgcctg ttcaaattgg tctcgttgaa tatttgtttc cgttagcttt
    25141 ggatgaggat cttccatgta aagttgtgga ctgacatctt gaccacctgc aagaataagt
    25201 ttgtcgattt gtccaatcag ttgaggagct gaatctggag acatgactgg cagaataaga
    25261 ggcaatcctt gagcatctaa aactgcatcc acgaatcctt gcggtgtata gctcacttga
    25321 tttccttgga aagtatctgt tgctcgaatc agttgatttc cggcaatgcc gatgattggt
    25381 ttcatttgta ttccccctaa agtagactta ttgtaacaag gatttaaaaa aaattaaact
    25441 atgtaagact tgcccctata cttatctgtt cttgctgtgt tactatctgt aaagagaaat
    25501 ttcgtgtgaa attcatgacg atagttgaaa atattgctta atttgttata ataaaaagta
    25561 agcagaaata aaaaggttgg gcgtaagctt caaggatcta gttcttcaag gggtgagaag
    25621 gacattttat tgaaggggcc tgcctatact aagttatctt tagaggtaca tactttcaat
    25681 gaaaaaagaa agcatgtacc tcttttttaa atcgaaagga agtgaagcaa gtgttagaag
    25741 ctttggaaca aatccgaaaa gcagaagaag aaaatgaaaa gcgtgctcaa acactgcggc
    25801 agcagctgaa gatctatgca acagaaaaag agcaggaatt ggaaacgatc caaacaaact
    25861 atcgacaaca actaaaacag ttggtggaag aaaaagaaca atttgaaata caagaattag
    25921 aaaaagaaaa aatgaaattg aaagcagaag cagacaaggt gcaagcaact ttgaaagatc
    25981 aatatcaaaa gcatcagagt caagcgatcg gtgcgatcat tgaaagggtg aaagaaactt
    26041 atggccgtca ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg
    26101 attttacaag cagtacaagg aatccaaaac gttgaaatcc gggatctgta tcaaaataga
    26161 acaaacaacc agtgggtgga gaagtatttt ccagatacca cgattattga taaagaagca
    26221 aaattaagcg ctttaggtaa tcggttgaaa gacatccaag aagctatcca attcatacaa
    26281 caccacggcg atagccgtca aaagaaactc catttgaaac gtcaagaact ttccttacaa
    26341 gatttggaag ccaattattc cgaagaaaat ttcataaata aattgaaaga agtccttgat
    26401 ttaaaagaaa agtgggaaca actaactgaa gaacgtgatc gtttagaaga agaggaagaa
    26461 tggcttcttc attggcaaca gctggatatt gctccaagta tgtatgcaag taagacgacc
    26521 atttttgaga tggggactgt gagtgcagca aattttgaaa gtttcaagga agaactgagt
    26581 aagcttgatg acgtttatat ggaagaaata gattatacgc caaaacaagt ttatctttcg
    26641 tatgttgctt taaaaaaatc agttgctgtt atagaagaaa ttgctgggaa atacgggttc
    26701 aataaagaaa cgtatcctta tgaacaaaat cctaaagaaa aactgcaaga gaccaagcag
    26761 caattatcca ttgttcacga gcagcaaaag aagataacga cagcgttagg tcagtgcagt
    26821 ggctatatcc aagaatttga atgggtagaa gaagtcacac ttgccgttgc agaacgagaa
    26881 aaaataaaag aacggtttat ccatgcgact tatctgatcg ttttgcaagg ttgggtagat
    26941 gcagaagaaa aacagaattt gctgcatgtg ttaaacgaaa ccgtatcaga agaagatata
    27001 tacgtcagtt tcgaaacacc aaactcagaa gagattcagc aggaagtccc tactaaattg
    27061 aaaaaccatc cgcttgtcga accttttgaa ttgctgactg aaatgtacag tctgccaaaa
    27121 tatgaagaag tagatccaac accttggatg acaccatttt atcttgtctt cttcgggatg
    27181 atggtcgcag atgttggtta tggtttgctg atgctcattg ggacaatact agctcaaaaa
    27241 ctacttgttc taccacgcgg gatgaaacgt ttcgtaaaat tctttgagat tctctcgata
    27301 ccatcgatca tctggggatt gatctacagt tcatttttcg gacagtcatt accaaaagaa
    27361 ctgtttggca tccgcttgcc tttcccaatc ctatctacga cggatgacgt caatacgatt
    27421 ttgattttat ccgtgatttt cggtctgata cagattttgg ttggattatt tgtggcagca
    27481 aaggaaaata tcaggagaaa agattacttg agtgctgtca gtgatggatt tgcctggcaa
    27541 gggatcctaa ttgggatcgt tattgcgttg gttggtgcca tgttgataaa aaatacagca
    27601 tttgtttacc ttggcgctgg gctatcgatc attgctgcct tatgtatctt gatcgttccg
    27661 attatccaaa cgccgtctaa agtcaaaggt gctgccaaag gtgcgtataa tctttacgga
    27721 ctgaccggct atatcggtga tttagtcagt tatacacgtt tgatggcatt gggtatttca
    27781 ggcggaagta ttggagcggc attcaatatg ctagttgctt tcatgccgcc agctgctcga
    27841 ttcagtgtag ggatcctttt gatcatcgct ttacacgcac tgaatatgtt tttgacactg
    27901 ctaagtgcct atgtccatgg cgcacgattg cagtatgtcg aattttttgg gaagttttat
    27961 acaggaggcg gacgtgcctt tgatccgctg aaaacggcgg aaaaatacgt caacatcaat
    28021 cacaagaaaa aaaacaataa ataattggag gatttttata taatgatgga ttacttaatt
    28081 agtcaaaatg gtggaatggt atttgcggta ttagcgatgg caacagcgac gattttttca
    28141 gggatcggtt cagctaaagg tgttggaatg acaggtgagg cagcagcagc attgacaaca
    28201 agtcaacccg aaaaattcgg gcaggctctg attctgcaat tgcttccagg cacgcaaggt
    28261 ttgtatggat tcgttattgc ctttttgatt tttatcaact taggaaatga catgtcagtt
    28321 gtacaaggac tgaactttct aggtgcttct ctgccgattg cttttacagg cttgttttca
    28381 ggtattgccc aaggaaaagt cgcagcggca ggtattcaaa ttttagcgaa aaaacctgaa
    28441 catgctacaa aaggaatcat ttttgctgcg atggttgaaa catacgcgat tttaggtttc
    28501 gttatttcct tcctattagt gttgaatgcc taagcataaa ggaggaaaag cagatgaacg
    28561 ccattgaaaa aatcatctct cagatgaacg aagcagcaga gcaggaaaga gcggcgttag
    28621 aacaagaaga gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg caagttgaaa
    28681 cagaacatca aaaacaaaaa gaaaaacaaa tcgaattact cgaaaaaaaa taccgtcagc
    28741 taaggaatcg ccagcaagta gaagtacgac aagaaaattt gaatgcgaag caggaatttt
    28801 taagacgatt gtttgcagat gcggtaactg aaatggaaaa ttgggatgaa tctgaacaaa
    28861 tccagtttat caaaaatgca ttgtattcat tacctttgac aggaaaagta gcattcattg
    28921 ctggagaaaa atcagcagcc tatctttctc agacgttgtt ggatgaatgg aacaatgaac
    28981 ttccctttat gatggtttta agtgatgaaa ctgttgctga tcaagcgggt tttctgataa
    29041 acgatcaagg agttcagtat aattttcttt tcagcagcct tgtacaagat atccaaggaa
    29101 cgatgagttt tgagattgcc aatcaattat tcgaataagg agggatcagt gtatggaata
    29161 ccacgaacta aatcccttga ttcgcggaag agagcttgaa cttttgtcca atgagacatt
    29221 tgaacggatg attcaaacaa attctgtcga agcacttggt gaaatattga aatccacgat
    29281 ttacagtcca tatatttatg acggatatga atatgacttc gaacagaatc ttttaaagga
    29341 gcagagccgt ctatttcgct ggttaaagga atcagcaccg gagcctgaaa tcgtttggat
    29401 ctatacgatg cgttatactt tccataatct gaaggttttg acaaaagctg agatgacaaa
    29461 tcaaaatctg gaccatttgt atatcgacga tggattttat tcattagaaa ccttgaagga
    29521 tgccattcat acgcaagttt caaatgaact tcctgaaagc attatggaca gtatacgaga
    29581 agtacgtgat tattttgaag agtctaccat tcttcaggga attgacgtga tttatgatcg
    29641 acagtttttg acagagcagc gacggcttgc cgataagcta ggatatcaag aattgctgac
    29701 agaagtcatt gcttttattg acttgaccaa tatcacaacg atggctcgag gaatattgca
    29761 gaaaagaaca tatgggttca tgacgacggt actttccagc tcgggaagta tcgaaaaaga
    29821 agtatttttg aactttgtag ataaagatat ggaagattac acgaattttc tattagagac
    29881 agattatgct gattttctgc gccctgtcat ccgtgaggga aatattgatc tcgtaaagct
    29941 tgaacagttg aaagatgatt atctaagttc attgtatcaa gaagcacaaa cccaggcttt
    30001 cggaccactg ccactactag cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg
    30061 tttactggtt atcggcaaaa gaagccattt cgaatcagaa acaatcagaa agcgggtgag
    30121 acaagtctat gacgcataag atcggtgtag ttggcgacaa agattctgtt ttgccattca
    30181 aattgtttgg ctttgatgtt cgttatggta caaccaagca aaccgttcgt caggcaattg
    30241 aagaaatggc aaagaatgag tatggcgtga tttatgtcac agagcaatgt gcagcattga
    30301 gcccagaaac gatcgaacgt tacaaggaac aaatgatacc tgctatcgta ttgattccca
    30361 atcatcaagg atcattaggg atagggctgg aagaaattca aaaaaacgta gaaaaagcag
    30421 taggtcaaaa tatcttatag tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca
    30481 taaaagtctc cggacctctt gtaatggctg agaacatgtc tgatgccagt atccaagaca
    30541 tgtgtctagt gggagatcta ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg
    30601 cgtctattca agtatatgaa gagacttctg gaattggtcc aggcgaaccg gttcgctcta
    30661 ctggtgaagc tttatctgtt gaattaggac cagggatcat ttcacaaatg tttgacggaa
    30721 tccaacggcc attagatacc tttatggaaa tcacacaaag taatttctta gggcgcggag
    30781 ttcaattgcc cgcattggac catgagaaaa aatggtggtt cgaaccaaca gtagaagccg
    30841 gagaaacggt gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa gttataaaac
    30901 ataaaatcat ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa aaaggttcat
    30961 tcactattga tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga
    31021 tgcttcaaaa atggccagta agacgtagtc ggccaatcaa agaaaaatta aacccggatg
    31081 cacccatgat cactggacag cgagtgattg atacattctt cccagtgaca aaaggaggag
    31141 cagcagctgt acctggtcct tttggtgcag ggaaaacagt cgttcagcat caaattgcaa
    31201 aatgggccga tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga aatgagatga
    31261 cggatgtttt aaatgaattc cctgaattga ttgaccctaa tacaggagaa tcgttgatgg
    31321 aacgtacggt attgattgct aatacatcaa acatgccagt agcagctcga gaagcttcta
    31381 tctatacggg aatcacaatt gcggaatatt tccgtgacat gggttatgac gttgctatca
    31441 tggcagactc cacttcacga tgggcagaag cacttcgtga gatgagcgga cgtttggaag
    31501 aaatgcctgg tgatgaaggt tatccagctt atcttggttc acggcttgcg gaatactatg
    31561 aacgatctgg aagagtcatt gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa
    31621 tcagtgctgt atccccgtct ggaggggata tttcggaacc agtcacacaa aatacattgc
    31681 gggtcgtaaa agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt
    31741 cgatcgattg gatacaaagc tattctcttt attcaacaga agtagggaag tatatggatc
    31801 atatccttca agctgactgg gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag
    31861 aacagcagct gaatgaaatc gtgcgtttgg tagggattga ttccctttca gataatgacc
    31921 gtctgacatt agaagtggca aaatccattc gtgaagatta tttgcagcaa aatgcttttg
    31981 atgatgtcga tacattcact tcaagagaga aacaattcaa aatgttgaag atgatcttga
    32041 cctttggtga agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg
    32101 acggtaccgt atcgattcgt gaaaggatcg gacgaagcaa atatatcccg gaaacagaat
    32161 tagaaaaact agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag
    32221 aaggaggcat gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt cggtccattg
    32281 atggcggttg aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa
    32341 aatggcgaga ttcgtcgtgg acaagtattg gaagtccaag aagataaagc aatggtccaa
    32401 atattcgaag gaacaagtgg tatcaattta cgtgactctt ctgtacgatt tttaggccat
    32461 cctttagaac taggtgtatc ggaagacatg atcggtcgag tattcgatgg tttgggacga
    32521 ccgaaagata acggaccaga gatcctgcct gaaaagcatt tggatattaa cggagaagta
    32581 atcaatccag tagctcggga ttatccagat gagtttatcc aaacaggtat ctctgcaatc
    32641 gatcatttga atacattagt tcgtgggcaa aaattaccag tattttctgg ctctggtctt
    32701 cctcataaag aattagctgc tcagatcgcg cgacaagcaa cggtactcga ttcatcagat
    32761 gatttcgccg tagtctttgc tgctattgga atcacttttg aagaagctga atttttcatg
    32821 gaagattttc gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac
    32881 gaccctgcaa tcgaacgtat cgctactcct cgtatggctt tgactgctgc agaatatctt
    32941 gcttatgaaa agggaatgca tgttttggtt atcatgacag acatgaccaa ctatgcagag
    33001 gcattgcgag agatttctgc agcacggcga gaagttccag gaagaagagg atatcctggg
    33061 tatctttaca cgaacttagc tactttattc gaacgagcag gaagaatccg cggactaaaa
    33121 ggatcggtta ctcaaattcc gattctaaca atgccagaag acgataaaac tcatccaatc
    33181 cctgacttga ctggttatat tacagaagga cagatcatct tgactagaga gctttacaag
    33241 agtgggattc agccaccaat tgatgtactc ccgtctcttt cgcggttaaa agataaggga
    33301 actggacccg gaaagacaag agaagaccat gcagctacga tgaatcagct ttttgcagcc
    33361 tatgcacaag gaaaacaggc aaaagaatta gccgtagttt taggtgaatc tgctttatct
    33421 gatgtagaca agatctatgc gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa
    33481 ggattctaca cgaaccgttc gattactgaa acacttgatc tagggtggga actgctatcg
    33541 atgttgccaa gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa atatctgcca
    33601 gaagggaagt gagttctgat ggcacgattg aatgtgaatc cgacccgcat ggaattgact
    33661 cgattgaaaa aacagttgac aactgccaca agagggcata aattattaaa agataagcaa
    33721 gatgaactga tgcgtcaatt catcctgttg attcgaaaga acaaccagct gcgtcaagaa
    33781 atggaagagt ctatgacaga agcaatgtct gattttgtat tggcaaatgc ttcgatcacg
    33841 gaacctttca tcgaagaatt atttgttttg ccggcggaaa acgttgattt gtctatcgta
    33901 gacaaaaata tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga tgaacagctg
    33961 acagacacac ctttggaata cgggtatttg aattccagtg ccgaactgga tcggtcaatc
    34021 gatcggttta ctaccctgct tccaaaactt ttggaactga cagaaatcga aaaaacttgt
    34081 caactgatgg cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg
    34141 acgatccctc aattagaaga aacgatctat tatattcgta tgaagctgga agaaaacgag
    34201 cgcgcagaag taacaaggtt gatcaaagta aaaaatatgg gtaccactga ctaagtggta
    34261 tcttttttta aaagaggaaa acgatgatta aacaaagcaa acaaataaag aaacgtttgc
    34321 cggctgttca gcttctcgca ttaggatttt ttctcctgat tttagttggc ggcggcctac
    34381 ttaccctacc attttttagt aatagtgggc atgggacaaa ttttatcgat gcgcttttca
    34441 ctgcaacttc tgctgtctgt gtaacgggat tgacaacatt gaatacagca gagcattgga
    34501 acagtgctgg tcaatttttg atcatgcttt tgattgagat cggcggtctt ggttttatga
    34561 tggttccgat tctatgcttt gcattgataa agaagaaagt cagtctcagc acacgtatta
    34621 tattaaaaga agcgctgaat ctagaagaag tatcaggtgt gatgaagctc atgctttaca
    34681 tcctaaaatt ggcctttgtg atccaattga ttggtacggt tgctttatct tttgtatttg
    34741 tgcctgaatt tggctggttg aaaggtatct ggtacagttt gttccatgca atctctagtt
    34801 tttgtaatgc agggtttgat ttgttaggag acagtttagc caatcaccag caaaatgttt
    34861 atttgatcat ggtggtatct gctttgatca tttctggtgg gttaggcttc atcgtatggc
    34921 gtgatttact ggaatatcat cagaaaaaga agatgacgat tcattcaaaa atcgcattaa
    34981 ccgttacagg taccctttta ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg
    35041 gggcaacgct agtggaaggg aatatgtttc aacgattggc aaacaccttt tttatgagtg
    35101 tgacacctag aacggctgga tattattcag ttgattacct ccatatgagc catgcaggtc
    35161 tgattttaac gatgtttttg atgtacatcg gcggaacatc tgggtcgacg gccggcggat
    35221 tgaaaacaac tacgttaggt gtgttgctga tccagatgcg ttcgatgttg aaaggacgta
    35281 cgagagcgga agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt
    35341 tattttttgt tacgttgacg ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa
    35401 cgatacctaa aacttctggc atcgaatata ttgcttttga ggtcttctct gcgtttggta
    35461 cagtaggtct tacgatggga ttgacgccag acttgactgc aatagggaaa ttgatcatta
    35521 tagcgcttat gtacattgga cgagttggtg tattgactgt ggtcttctct ctgatgacaa
    35581 aagcacaaag aacacaatca aaatacaaat atccagaaga aagtgtcatg attggataat
    35641 ttcatcttct gacagaaacc tagtgattta gtaaaataag aaaataaaat aatttttctt
    35701 gcttcttgga tactttaagt gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt
    35761 gaacctcggg tagtgcctct tttttattga aaggaatcaa taagatttga ttcctttatt
    35821 tatcaatagg ttataattca tattttttta ttttttcttt tacgatactt agtccagaag
    35881 ctaaaatttc aaggttatta gaggataggg aaattcgaac gaatgcatct ggttgcgcac
    35941 gtgtagtaaa gcgtctggaa tgataaagtc ggataccgtt agcaagaaag tcattctcaa
    36001 ttatcttagg gtcaatctca ggatcaactg gaagaattcg atagtaaggg acacgattcg
    36061 ttggtttttc tactttgaaa agagaatcaa aaagctgatt ggctcttttt gtaagactta
    36121 atttagtttc taagatccta tttgcttcat ttgaatggag gatttgagtg atgacttcca
    36181 catctaatcc agaagttttc acatttatat taaaaattgc ttgttctatc ttgctttgga
    36241 catgtactgg aactacaaga taagcaactc gcaatccaga acaaataaat tttgtcattc
    36301 cagctaagta gattgtttgg ttaggtaata aattttggaa aggaggaatg atcgtatgtt
    36361 gatagtaagt tgttaaaaat gaatggatat catcttcaat aacccataga ccttcttttt
    36421 caatcacttc ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct ataggattat
    36481 tacaagaagg cataagaaag acaccatgaa tcttcttttt tctacattcc tgaatcaata
    36541 tatcaggtcg cattccttgc tcgtcaaaat caattggaac gatctctaaa tgatataact
    36601 gagccaattc aatgaaatta gcgtatgtat atcggtctgt tgctattcta tcacctggag
    36661 agaaaagtgc agaaagtgtg atagccaatc cattttgaac accggaaaca atagatagag
    36721 attctaagga cggaatgact ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa
    36781 aatttgtgcc tttgggatct tggtaattaa gcaaaccaac aagatttggg ttattactag
    36841 ttgattgaat aaacgggaca acgaatttat tacattcttc aaaagaacta accagaccaa
    36901 aatcaatgat atgttcggtt acttcattgg tagagatggt gatggactca aagacattgg
    36961 gagaaataaa tgtgccactt ccaatattcg tatacagcaa acctttctca attccatatt
    37021 tataagcctt gccaacggta gtaaaattta aatctaaata atcagctagc tctctttggg
    37081 agggtaactg agtgtttttc tgtaatttat tctttaaaat atcttgttct aattgttgaa
    37141 tcaacgatcg ataataagga cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt
    37201 aattgtcata agaattaata ggcattttct ccactcctta aaattgtata ccatacaatt
    37261 ttaccattga ttgtattgat tttagaagtt aatcttttgt gaggagggaa ttgtctatga
    37321 aaattattaa ttttgaggaa gtagtagctt cttttagttt tgaagaagcg attcaagtaa
    37381 tgaaaaaatg tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg
    37441 aaattttacc tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct tatttggggg
    37501 agaacagata ttttggcaca aaagtcatta ccgcttttcc ggataatcat cagtatcatt
    37561 taccttctca tttaggagag attttcttgt ttgatgctca aaacggacta cccaaagcat
    37621 tgataaacgc aaatgcagtc acctggattc gaacagctgc tgtatctgcg ctggcaacag
    37681 attacttagc aaatccgcat gcagaaacac ttgctttaat tggagctggt caacaagcat
    37741 tgtcgcattt gactgcgatt tctaccattc gtcccatcac tacagtgttt gtttacgata
    37801 ttttagaaga aagacgaaat acttttatcg ctaaagctaa aaaacagtat cctaaaataa
    37861 cgtttataaa tgcaatatca gtagaagatg cagtacgtcc agcggaaatc atttgtacgt
    37921 tgacctctag taaagaggca tttcttcaaa aagagtgggt aagggaagac gcacatatca
    37981 atgctgtggg cacatttact cctgatacaa gagagataag tacatcgttg atggaaactt
    38041 ctcaaatcta tgtagatgat tactcagctg ccttaacaga aagcggggac ctgcttatag
    38101 ccatggcaga aggagattta gaggaatctt gtattgttgg tacattaggc gaattagttt
    38161 ctgagaaaaa aacattttat aaaggagaat cgaggccgac gattttcgac gcagttggtc
    38221 tagctattga agatctttgt tgtgcagaat atatttataa caaattagag aaaggggctt
    38281 taaaatgaac ttagattatt acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa
    38341 tccagcagcg gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc
    38401 cattgaaaac aatttatctg aaacagcatt tacagtgaag aaaaaccaag aatttgagct
    38461 tagatggttt acgccagatc gagaaatcga tctttgtggt cacgcgacat tagcaacagc
    38521 atttgtttta tttaactatt acaaaatacc tgatgaaacg attaaatttt ctactcaaag
    38581 cggtaatcta tttgtgacaa gaattaaaga ttattattac atggattttc ctagtattat
    38641 gccgaaaaaa gtgccgatat tgtctgaata tgaagaagcg attggggcta agattaaaga
    38701 agcctactta gcacgtgatt tatttttcgt attagaagat gaaaaaacgg tggctaaatt
    38761 gcagccagat tttacagcta tcaaaaattt tgaattagga ataggagtca tcgtgacagc
    38821 agaaagtgtg caaaaagact ttgtttctag aaccttcttt ccaaaattaa caatcaatga
    38881 agatccagta tgtggatcag cacattctaa tcttattcct tattgggcag aaagaatgaa
    38941 taaaacaaaa ttgtcagctt atcaaatgtc atcaaggggc ggggatctag aatgtgaatt
    39001 aaaagaagat cgtgtgataa taggaggaaa agcagttttg ttttctaaag gtacctcgtt
    39061 tatttgattt ctttcaaatg aattaaaaat aaattggtgg agagtggcaa ccttatagct
    39121 acaatggttg ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt tgcgtctcat
    39181 tttaattatt ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat actaactatc
    39241 ctcggatgac ttcgatttta taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag
    39301 tgccaggtaa tccttttaag tcagtaatct caagaccagc agctttatgt tgctcgtgta
    39361 atttttctag atcatcagct gctatagcga tatgaccata gccgtctcct agatcatacg
    39421 caccatgatc atagttgtaa gtcaactcta attcatagtc atccccagga agtgtcaaat
    39481 aaacaagtgt aaatttgttt tctggaaaat cacggcggcg gctttcttca aaaccaaatg
    39541 ccttttgata aaattcgatt gatgcttcaa gatctttcac tcgaacacat gtgtgagcca
    39601 ttttcataga ttcatctctc cttttccttt tactcaatca taacaaaaag aagtaaggaa
    39661 atagttgatt tgtgcttgtt catgagaaat ttctcatgta aaatggatag aaaataaaac
    39721 tgttaaggtg gaaactattc atggataaac caatttttgg tgaaaaaaag caatttgctg
    39781 attatcaaac ccgttatgca gcatatattg tcatagaaag agatcagcag attgcagtaa
    39841 tcgaggctcc aaatggtgct ttttttcttc ctggcgggga aattgaagga gaagagacga
    39901 aagaagaagc gattgtccgt gagctgatag aagaaatggg aattgtggca gaaatttctt
    39961 cttatctggg agaagctgat gagtattttt actcgaatta tcgtgcaact tattattata
    40021 atccaggata tttctttgtt gctgattctt ggaagcgtat aggagaacca actgaaaaga
    40081 ccaataaaat ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc
    40141 accgatgggc agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat
    40201 cagctttctc tcagtttttt tatttattac agtacatatt ttttgattgc ttcagctaca
    40261 ccgtgctcgt cgtttgatga agtgatgaca tttgctgctt ctttgacaag agaaacggca
    40321 tttcccattg ctactcctag accagcatat tcaatcatgg ataagtcgtt ctcgttatct
    40381 ccgatagcca tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc
    40441 gcacctttac ttgcttcttt atttagtact tcgaagtaat agggcgcact tttgaccgtt
    40501 gtgtatttat cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt
    40561 tcgtcgatca tcatcatttt gacgatgttc atgtctggtg tcatttcttc aggtgttcga
    40621 tattttagcg gcatcttgac taaacttgct tcgtggatcg tataagcgct gatatctcga
    40681 ttaggcgtgt agatcgcttt gtcatcgatc gaatggagat gactgccgac acggcgtgcc
    40741 attacttcga tctctaggaa atcttcatgt gtcagtccgt gacgggcgat gattttacct
    40801 gatttcgttt gttgtacgag tgccccgtta taagtaataa cgtaatcatt atcttgaaaa
    40861 aggttcaact cgtttaactg ttcctggaca cctggtaagg gacggccagt gcataaaacg
    40921 atcgaaacac cttttgcaac agcagcggca atcgcttctt ttacttcttt tgttacttct
    40981 cttttctcat ttaataaagt accatcgatg tcgatagcga ctagtttgat agacataatt
    41041 tcctccaata tattttaatt tatttcaatc aatgcgccat tacggatatg gctggcaaat
    41101 tcttgataag tttcgtcaaa taaatcataa tgatcacgca aggacggatc aaccatttct
    41161 tttggaaaga aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc aacaagctga
    41221 ctaacctgtg ataattctat caaagttccg tcatttcgca aaatctcgat ttgtgtgcga
    41281 tgtctgcctt gttctgggcg ataaaaatca taaggtagat cgtagctaga attcacagca
    41341 gtgtaataaa caggattata tccgaccttt tctactaaca aagtgagttc ttggactaat
    41401 tttgaatccc ggttgccaga aaaagtagca gatttcagtg gtttacgatt caaaaaacgt
    41461 ttagctaaat catttaaaat aggatcagca gattcagacc attgagtaaa gtaagtgccc
    41521 aagaccccat catcaagttt gaggtattct tctaaagtaa agcttccgtc aagaaatggc
    41581 agaagtaatt gagaatggag ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg
    41641 cgatgaagca gatgctccaa aattacttcc ataccacggg aaacaggatg gaagtacact
    41701 tgcacataca tttgatatcg gctaacgata taatcttcca ctgcatgcat gccactcatg
    41761 gaaaaagcga tcccgccttc ataaggacga atcacacgta atattcgggt aagatcaaat
    41821 gttccatatt ctgttccagt gaaataagca tcccgaagca aatagtccat tcgatctgca
    41881 tcgatctgac tagaaatcat ttggacaact tgtgggttgg ggtaagtttt ttggatcaca
    41941 ctagctacct tttcaggaaa accttcttct actcggttaa gaatctgata cacttctgtt
    42001 tctggagaag tgatgatctc tacagtgatg gcttcatgat tcgtatggaa gatatgttca
    42061 aatgtatgag agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc
    42121 agccgttcct gatcatccca gcctccgttg cctatttttt cttttgaaaa atttcgagaa
    42181 aagatatcgc agattcgtct tgaaatctca taaacgccta atgaatgagt aaaacggcta
    42241 tgttctgctc cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt
    42301 tggacttctt tcgaattgat caaatctaag attacttgat gctggacatg gatatagtta
    42361 tggactggat cacgaaaaac tttttccata ggcagcattt gatatttata aggggttgtc
    42421 ataatgtagc tcctttatgt tattacgttg ttccattctc tggatttggt tgttccattg
    42481 ctccagctgg atttcttttt ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata
    42541 cagcttttca aaagcttttg caaatgcttc tttgacttct tttacaaaaa ggggttggtc
    42601 aagcagttct tcaagtgttg ccattgtagc aggatccact gcaggatagc catttgtacc
    42661 aaagtcttct tttaaagcag attgataaaa tcgcttcatg acttgcccac gtttttcctg
    42721 attaccgttg acacttaagt acatcataac agaaatccca tttttgatcc gacgctgagc
    42781 gataccggca aattttttgc catggatgct taaatcataa gttccaggac aataagaatg
    42841 ggtgatttcg aatgcttgta tcggtgcctt tggaaaagcg tatttagtca ataggagcat
    42901 ttgctcgtaa gcgtcatcta tggttatttt ttttgtgtca ctttggggga aaatcaaaga
    42961 aatattcaaa attccttcat ctgatacgat ccctaatcca cctgaatttc tgatcactgg
    43021 agcgtaacct gcatcttcca gtgtttggat cccgtcactt agaaaaggag ttcgaacatc
    43081 cttcatacct aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg
    43141 ttctttagaa tattccgtta atatatctgt taatgcaaaa ggggaaaagt cgtttttttg
    43201 ataaatgtgc tgatctagtc gaatcgcagg gttcattcaa ccggctcctt tgtataaatt
    43261 catattttct acaatattat actctaaaat caaagagttg ttttggaaaa aaggttttct
    43321 gtaaaaatgg acaagttggt tgacaaaaca aaagaaacta ggaacaatgt agctatgaga
    43381 agtaaaggag aatgaataat ggaattatca caaggaacac cgatttcaat caaattgcgt
    43441 acaaaagtaa aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg
    43501 gtgacaattg gcgatacgct gtacatacgt tataaagaga ttcaggagga gacgggcgat
    43561 gagattcctg taacgatcaa attagttcca gatggtcatg tacagctgat ccgtgcagga
    43621 gaaatgcgga tgcgtttgaa atttggctat aaagaacggt tagaaacatc ctatcgaaca
    43681 ccatacggta tgatccagat tgctactttt acgaaagaac tgcatgttag tttgaaagac
    43741 cgtcccacag cagggaaggt tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc
    43801 ggcgaatatt atttgacctt ggattttaca gcgtaaatat aaaagaaaag tttgattttt
    43861 tgtgtctgat tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt
    43921 agtgtatttg aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc
    43981 ttagaacaac gtggcgatgt catggatttt tctgatttag ctaaccagat ccaaaactat
    44041 cttgggaaat cagacagtga aattcgtgat tcactcgcac aattttatac tgacttgaat
    44101 attgacggca gctttatctc attaggggat aatcgttggg gcttgcgttc atggtatgct
    44161 atcgattcga tcgatgaaga agtaaaccat ggaatggacg aagaggatga agatactcca
    44221 cgtcgccgta aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat
    44281 tacaatgacg atgacccaga agatacagat ctgacagaag aagatgacga tgatctgttt
    44341 gatgacgatg atgatgaaga tgaagaaatc gctgcttata attctgactt gcaagaaatc
    44401 ggagcagatg acccgacaga tgatgaagaa gacttgccag gaaatatcga agaagattta
    44461 agtatcatag acgaagacga tgatgatgaa gattatgaag aggaagaatt ttctgactct
    44521 gaaaaagaat aaacattatt ttcttacgag aggttgtgac agagacgatc agcctatcag
    44581 aataaggagg tgtgacacga tttttgtcac accttctttt ttgtatataa aaatcccaag
    44641 taaatacata aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt catattaagt
    44701 gaggcagaaa ggagtggaga atgattttta ctggatataa gaaaaaacgg cagatcgaaa
    44761 aataccgtca cctgctgaat ggactcatcg aaactgatta tcaaaaaatg ttccgtatca
    44821 cgatgaatta cgtacatgat aaagaagagg ccttagatgt gatgcaagat agttttcata
    44881 aagccctgaa ctattttgaa aaagaaaaag agatcgagca ttttagtgca tggttttatc
    44941 ggatattgat ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg cctgtggact
    45001 tatttgaaat tcaagatatc ccccaacagt tacctttaga aacttccgtt gcagagctcc
    45061 atatgatttt agataaaata gacagtccgg aaaaagagat tctgatcctc tacttttttg
    45121 aaggatttcg tttgaaagaa attgcgacta tcttggatat gaatgaaaac acagttaaaa
    45181 cgaaaatgta ccgctcattg aatgctttac gtcaaatcct agaatgaagg agagaagagt
    45241 gccatgtatt cccaaaaaga aaatttaaaa aaactaaaga aaacttataa agaacaagca
    45301 attcccaaag ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga gcaatcattt
    45361 ataaaagcaa agcggagaaa gagacgtctc caagggttcg gagtatcttt ggctatcgta
    45421 atgattacaa caagtagcct tctttttaat agtcaagttc gaagctttgc agaagattta
    45481 cccatcttag gatcagtcat tgaattgatc ttaggcgaac gctttactga tcgatctgaa
    45541 aaaatcgata ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa taaaacaatt
    45601 catggattaa atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa
    45661 gaatatggaa attttgaaac ggatcattac caagttttgg gagattatca gaaaatcgta
    45721 gacgataacc ggtttctcgt gattgaaagg cagatcaccc aaacagcagc agatagccat
    45781 gttgaaaaaa gatacgacac aatcgacaag aaaaacagtg tccagctctc acttccactt
    45841 ttattcaaag atgacaccta tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc
    45901 gaacaagtca aggaagatcc atctaagtac tattggactg agcaagatat tcaagaaggg
    45961 acgatagaaa aaccaacact tgttacgcct acacgaagtt tttatttgaa caaagagcat
    46021 caattagttt tgactttttc tcaatacgaa attgctcctg gttatatggg tacaccagaa
    46081 tttgtcattc ctaagtccgt gacaaaaacg attttggcat cggaagatta tttggatcat
    46141 tagacgagaa gtagttgcga ctagcagttt caattttcaa gtaagtacaa gtaaataata
    46201 agaagagaaa aagagaaaga agagatatag tgaaaagaaa ttatttagtt atcagtatta
    46261 ttgctttatc cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg atcaccgaag
    46321 taaaaggaca aacacactct tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat
    46381 caaccattaa aagccaatca actgaaacag ctaacgtcaa agaagccatt caagaatcag
    46441 cggaaaaggt acaaagtcag tcatcacaat caacacaaga cagtacaaaa acgagccgag
    46501 atccggctgt caaaagcgtc gcaatcagct ttgatgatgg accaggagcg accacaacac
    46561 cgcagcttct gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag
    46621 aaaatacggc gcagcatcct gaaatcgtga agcagacagc tgaagcaggg cacgagattg
    46681 gcaatcacac atacgatcat cagaatctgg ccactctttc tgcccagtcg atgacggagg
    46741 aagtgacgaa agcagacact gagatcaaaa aagtcactgg caaaaccccc acttttgttc
    46801 gtccgcccta tggttctgtc acgaatgtag gagcaacgat catccagcga ccgatcatcg
    46861 aatggtctgt tgattcagaa gattggaaga caagaaatcc tgatttgatt ttgcagaaga
    46921 tccaagcaac agtatacgat ggggcaatta ttttattcca tgatatctat cctgaaacga
    46981 ttcgtgccgt tcctcaagtg atcgattatc tgaaagagca agggtatcgc atcacgacag
    47041 ttggggattt attagggcat ccaacggctg tcgagaacta ttatggcaga aacgatcatc
    47101 ggccagttca gtaagtgaga caagtatctt taataaataa cacattttaa taagtagttg
    47161 aacacataac taccttactt gcaaaggctt aacaatatct atacaggtac aacaaaaaac
    47221 tgtccaccaa ttttctttgg cggacagttt tcacttaaaa ataaagaagc aactattcgt
    47281 aaattatgct aaaatagcag tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa
    47341 ccggaatctt acgtgatatc cactacacta aggacgcaag cactgggata attgtaacta
    47401 aacagataaa aaattacaag gggattggac aaaaaaatga aatttgagca aaatttttca
    47461 caaaaacagc agcagacaca gaaactagcc atgacgcaac agttgcaaca gtccatccaa
    47521 gttttacaat ataacagtga ggagctgttg gcattcgttg agaatcaagc aatggaaaat
    47581 ccattggtgg aagttgttga gccggaatgg cagccagatt acataaaagc ttcttcatct
    47641 tcttatgaag gagaagagac aaattaccta aatcagattc ctgatacaaa agggtcttta
    47701 tttgattcat tgatcgaaca agtccatttg aattaccgag atactttttt gaggaaaatc
    47761 gttctttatc ttgtagaata catcgatttg aacggatttt tgacgattag tttagaagaa
    47821 gccggaaaag agattggagc tacgcctatc caaatgctcg atgcgttgac tctgattcag
    47881 caattagaac cagctggagt aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca
    47941 gaacgagatg attatgcgcc ggaattagct tatatcgtgt tggaagaatg ttttgaggaa
    48001 ctggtggagc gaaaatggaa agaaatcgca caacgttttt ctgttgatct acatgctgtt
    48061 cagcagatat tcgactattt acaaacgttg agtccaagcc ctggccggat tttcgaccgt
    48121 agcagcgaat tgtacattat tcctgatgtc cgggtattag tggacgacga taaaaatgta
    48181 caagttatct caaaccgaaa aaatcagcca aatatacgct ttcaagagag ttacttcaaa
    48241 caaatgtcac aacaagcaga taaagaaact gaaaattatt taaaagaacg gaaacaggaa
    48301 ttcgaatggt tgaagaaaac gatcctacaa cgtggggata caattttgcg ggttgctcaa
    48361 gtcattgttt cccggcaaaa agctttcttt atcgacaagg aacgtccaat caagcctctt
    48421 acgttaaagg aagttgcaac tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc
    48481 aacgggaaat atttggaaac agacttcggt gtatttgaat taaaaaaatt tttcactaca
    48541 cgaattccta cgaatagtac agaacagaca gaagaccttt cagcagatac agcgaagaaa
    48601 aagctgcagg aattagtcga tcaagaagac aaaaacaagc cgttgtctga ccagaaattg
    48661 gttgaattgc tgaaaaaaga tgagatagcg atctcaagac gaacagtagc aaaatatcgt
    48721 gatttattag gaatcccgag ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa
    48781 agactagaaa aagtatttca ctttttctag tctttttagt tgaaaaacga gagcaaaact
    48841 gttagactta cagtcgtgag gttgagattt ccctttattg atgtgaaatt cgaatagtta
    48901 atttcttttt ttatcgtata tgggtcgttt taagtctaca ttggacgtaa agtgtccaac
    48961 taaggagatc gctatgctgg atgaaataaa aatgattgaa tctgttgcac cagatattat
    49021 cgaagtgttg caagaacgct ataaaatttt acgaaatatc tattggatgc agccgattgg
    49081 acgtcgaagt ttatcggaaa gtatggggat cacagaacga gtattgcgaa cagaaacaga
    49141 tcttttgaaa aatctgaatt tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa
    49201 gggcgaagaa gtttatcaaa gccttgaaaa cttcatggat cagcttttag gtacgcatca
    49261 aaccgaacaa cagttggcag aatattttgg taaccaacgc tgtatcgttg tttcaggaaa
    49321 cagcgaagag cagaccaaag tggctgatgc attcggcgaa gcgctgagcg aagccctaga
    49381 ccgactactt ccagaaggcg agaatatcat cgctgtgatg ggaggaacta ctatggctgt
    49441 ggtagccgaa caattgtcta atctggaaaa taagaaacgg cataacctgt ttgtccctgc
    49501 aagaggcggg attggtgaag cgataactgt acaggcaaat tctgtcagtg caagaatggc
    49561 tgctaaagca aacggtaacc accgtgcact ttatgttccg gaacaattaa gtctggcaac
    49621 ttacaattct ttactcaatg agccatctat ccaagaagtc ttaaacttga ttagtgaagc
    49681 gaattgtgtt attcatagta ttgggcgtgc attacacatg gcagcacgtc gtaagatgac
    49741 tgaaaaagaa ttagtcatgc tgaaacaagc aaatgctgtt gcggaatctt ttggctactt
    49801 tttcaatgaa aaaggtgaag tcgtttataa ggtccctaga atcggcatag aattgcaaga
    49861 tttggaaaaa gtcccgatca tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat
    49921 tcgagcttat atgaaaaatg caccaaaaca aacgtggctc atcactgatg aagctgctgc
    49981 aaatgagatt ttaaaagggg taacccttta aaataaatat ttttttgatt ttcataagga
    50041 ggaaatctta aatgacagtt aaagtaggta ttaatggttt tggacgtatt ggacgtctag
    50101 cattccgtcg tatccaagat gtagatggaa tcgaagtagt agcaatcaac gacttgacag
    50161 atgctaaaat gttggcacac ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa
    50221 cagttgaagt tcatgaaggt tcattcaacg taaacggaaa agaagttaaa gtattagcta
    50281 accgcaaccc agaagaatta ccatggggcg aactaggcgt agatatcgtt ctagaatgta
    50341 ctggtttctt cacttctaaa tcagcagctg aaaaacattt aacagctggt gctaaacgcg
    50401 ttgttatctc tgctcctggc ggaaacgatg ttccaacaat cgtttataac acaaaccacg
    50461 aaacattaac tggtaaagaa acagttatct caggtgcttc atgtactact aactgtttag
    50521 ctcctatggc taaaacattg aacgacaaat ttggtgttgt tgaaggatta atgacaacta
    50581 tccacgctta cacaggtgac caaatgactc tagacggacc tcatcctaaa ggtgacttcc
    50641 gccgtgcacg cgctgctgct gcaaacatcg ttcctaactc aacaggtgct gctaaagcta
    50701 tcggtttagt aattcctgaa ttgaacggta aattagacgg agctgctcaa cgtgttcctg
    50761 taccaacagg ttcattaaca gaattagtaa cagttcttga aaaagaagta actgttgacg
    50821 aaatcaatgc agcaatgaaa gaagcttcaa acgaatctta cggatacaac acagacgaaa
    50881 tcgtttcttc agatatcgtt ggtatgactt acggttcatt atttgatgct acacaaacta
    50941 aagtaatgac agttggcgac aaacaattag ttaaaactgt tgcttggtac gacaacgaaa
    51001 tgtcatacac tgcacaatta gtacgtactt tagaatactt cgctaactta taagatagac
    51061 aaacgtacaa gtgtaaatta tagacatgta catttaaaag cggggaagca atcgcgcttc
    51121 ttccgctttt tttgataata agtttattca ggaggaaata aaatggctaa gaaaacagtg
    51181 aaagatatcg atttaaaaga caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg
    51241 aaagacggcg tgatcactga cgacactcgt atcaaagcag cattgccaac aatcaactac
    51301 gttctagaac aaggcggaaa agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa
    51361 gaagataaag aaggaaaatc tttagctcca gttgcaaaac gtctaggcga attattaggt
    51421 aaagaagtaa cattcgtacc tgaaactcgc ggagaacaat tagaagaagc aatccgcaac
    51481 atgaatgacg gcgacgttgt cgtattcgaa aatacacgat ttgaagatat tgacggtaaa
    51541 aaagaaagca aaaatgatac agaactaggt aaatactggg cttcattagg cgatgtattt
    51601 gttaacgatg ctttcggtac agctcaccgt gcacatgctt ctaacgtagg tatcgcttca
    51661 actggtatcc caacagttgc tggattcttg atggaaaaag aaatcaagtt catcggtgaa
    51721 gcagtagaag aaccaaaacg accaatgatt gcaatccttg gtggcgcaaa agtttctgat
    51781 aaaatcggcg taatcgaaaa cttgattcct aaagcagata aaatcttgat cggtggcgga
    51841 atgacttaca cattctacga agcaaaagga atcaagatcg gtaactctct agtagaagct
    51901 gataaagtgg aactagcaaa agaattaatc gaaaaagcag gcgacaaact tgtcttgcca
    51961 atcgacaatg tctgtgcacc agaattttca aatgatgttg aaacacaagt aatcgaagga
    52021 gacattcctg atggtttgat ggctttagac atcggcccag catcagtaaa attatttgct
    52081 gatacattac aaggtgcaaa aacagttgtt tggaacggac ctatgggtgt atttgaaatg
    52141 agtaactttg ctaaaggtac aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca
    52201 acaacaatca tcggtggcgg agactcagct gcagcagcag aacaattagg atttgctgac
    52261 aaattcacac acatctcaac aggtggtggc gcaagcttgg aattgcttga aggtaaagaa
    52321 ttaccaggac tagcagcaat caacgataaa taaacgacca agtttgctca attctgacga
    52381 gtaatcgatc aaaaggtgaa gggagttttc atgactccct agccattttt caataaatta
    52441 aggagtgtgt tcctcaatgc gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac
    52501 tgcatcagaa gcaaaagaat ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa
    52561 agttgattca gtcatcggat ctcctgcatt attcttacaa gaattaatga catcagctga
    52621 aggtacagaa ctaaaaatct cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac
    52681 tggtgaaact tcaccagcag ctcttgctga tctaggtgtt cattatgtga tcatcggaca
    52741 ttcagaacgt cgtgaatatt tccatgaaac agacgaagac atcaataaaa aagcaaaagc
    52801 tatctttgca aatggtatga caccaatcct atgctgcggc gaatctttag aaacctatga
    52861 agcaggcaaa actgctgaat ggatcgaagg acaaattaca gctggtttag ctgatctttc
    52921 agctgaacaa gtgtcaaaca tggttattgc ttatgaacca atctgggcaa tcggtactgg
    52981 taaatcagct gacgcacaaa tcgctgacga aatttgtgga gttgtacgtc aaacagttga
    53041 aaaactttat ggaaaagaag tatcagaagc agttcgtatc caatacggcg gttctgtaaa
    53101 accagaaaat attgctgaat acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg
    53161 cgcaagcttg caagcagatt catttttgtc attgttagac gctgtaaaat aattgttgaa
    53221 aatttgcgaa aaatgcaaga aatgtttgca ctaatgagca aaattaacta aaatcgtata
    53281 tgagggaaat cccttaggaa aaaacaaact caaaggagag acaaaacatg tcaattatta
    53341 ctgatgttta cgctcgcgag atcttagact cacgtggtaa cccaacaatc gaagtagaag
    53401 tatacacaga aagcggagct tttggccgcg gaatggttcc ttcaggtgct tcaactggtg
    53461 aatacgaagc agttgaatta cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa
    53521 ctaaagctgt cgacaacgta aacaacatca ttgctgaagc aatcattggc tacgatgttc
    53581 gtgaccaaat ggctatcgac aaagctatga tcgctttaga cggaactcct aacaaaggta
    53641 aattaggtgc gaacgctatt ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact
    53701 acctagaagt acctttatac cactacctag gcggattcaa cacaaaagta ttgccaacac
    53761 caatgatgaa catcatcaac ggcggatcac atgctgataa ctcaatcgac ttccaagaat
    53821 tcatgatcat gcctgttggc gctccaacat tcaaagaagc tctacgtatg ggtgctgaag
    53881 tattccacgc attagcttca atcttgaaag ctcgcggatt agcaacttct gttggtgacg
    53941 aaggtggatt tgctccaaac cttggttcaa acgaagaagg ttttgaagta atcatcgaag
    54001 caatcgaaaa agctggctat gtacctggta aagacgttgt tcttgctatg gatgctgctt
    54061 cttcagaatt ctacgacaaa gaaaaaggtg tttacgtact agctgactca ggcgaaggcg
    54121 aaaaaacaac tgacgaaatg atcaaattct acgaagaatt agtttctaaa tatccaatca
    54181 tctcaatcga agatggatta gacgaaaacg actgggacgg attcaagaaa ttaactgacg
    54241 tattaggcga caaagttcaa ttagttggtg acgacttgtt cgtaacaaac actcaaaaat
    54301 tatctgaagg tatcgaaaaa ggaattgcta actcaatcct tatcaaagtg aaccaaatcg
    54361 gtacattaac agaaactttc gaagctatcg aaatggctaa agaagctggc tacacagcag
    54421 ttgtatctca ccgttctggt gaaacagaag attcaacaat ctctgatatc gctgttgcaa
    54481 caaatgctgg ccaaatcaaa actggttctc tatcacgtac tgaccgtatt gctaaataca
    54541 accaattact acgtatcgaa gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat
    54601 ctttctacaa cttaaaaaac aaataattct tttctaatga agatacatga tcgactattg
    54661 aaagatagtc gagtatgatc acttagttaa gtaatcgaac tctctacttg ctgaaatatc
    54721 agcaggtaga gagt
//
LOCUS       DAZKCC010000010        53843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000010 DAZKCC010000000
VERSION     DAZKCC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.010"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(401..904)
                     /locus_tag="ACG8VH_000614"
     CDS             complement(401..904)
                     /locus_tag="ACG8VH_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9109197.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1263..2288
                     /locus_tag="ACG8VH_000615"
     CDS             1263..2288
                     /locus_tag="ACG8VH_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9109198.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2611..3489
                     /locus_tag="ACG8VH_000616"
     CDS             2611..3489
                     /locus_tag="ACG8VH_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP9109199.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3965..4081
                     /locus_tag="ACG8VH_000617"
     CDS             3965..4081
                     /locus_tag="ACG8VH_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9109200.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5009..5347
                     /locus_tag="ACG8VH_000618"
     CDS             5009..5347
                     /locus_tag="ACG8VH_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109201.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5441..5671
                     /locus_tag="ACG8VH_000619"
     CDS             5441..5671
                     /locus_tag="ACG8VH_000619"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP9109202.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6071..6442
                     /locus_tag="ACG8VH_000620"
     CDS             6071..6442
                     /locus_tag="ACG8VH_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP9109203.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6716..7408)
                     /locus_tag="ACG8VH_000621"
     CDS             complement(6716..7408)
                     /locus_tag="ACG8VH_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9109204.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7411..8196)
                     /locus_tag="ACG8VH_000622"
     CDS             complement(7411..8196)
                     /locus_tag="ACG8VH_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9109205.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8433..9833
                     /gene="lacG"
                     /locus_tag="ACG8VH_000623"
     CDS             8433..9833
                     /gene="lacG"
                     /locus_tag="ACG8VH_000623"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9109206.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9858..10175
                     /locus_tag="ACG8VH_000624"
     CDS             9858..10175
                     /locus_tag="ACG8VH_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9109207.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10188..11861
                     /locus_tag="ACG8VH_000625"
     CDS             10188..11861
                     /locus_tag="ACG8VH_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP9109208.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11875..13506
                     /locus_tag="ACG8VH_000626"
     CDS             11875..13506
                     /locus_tag="ACG8VH_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP9109209.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(13997..15562)
                     /gene="guaA"
                     /locus_tag="ACG8VH_000627"
     CDS             complement(13997..15562)
                     /gene="guaA"
                     /locus_tag="ACG8VH_000627"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9109210.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15621..16544)
                     /gene="coaA"
                     /locus_tag="ACG8VH_000628"
     CDS             complement(15621..16544)
                     /gene="coaA"
                     /locus_tag="ACG8VH_000628"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9109211.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16740..17345
                     /locus_tag="ACG8VH_000629"
     CDS             16740..17345
                     /locus_tag="ACG8VH_000629"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9109212.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17653..18669
                     /locus_tag="ACG8VH_000630"
     CDS             17653..18669
                     /locus_tag="ACG8VH_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9109213.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18810..20111
                     /locus_tag="ACG8VH_000631"
     CDS             18810..20111
                     /locus_tag="ACG8VH_000631"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP9109214.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20157..20819
                     /gene="deoC"
                     /locus_tag="ACG8VH_000632"
     CDS             20157..20819
                     /gene="deoC"
                     /locus_tag="ACG8VH_000632"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9109215.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20840..21238
                     /locus_tag="ACG8VH_000633"
     CDS             20840..21238
                     /locus_tag="ACG8VH_000633"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9109216.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21304..22386
                     /locus_tag="ACG8VH_000634"
     CDS             21304..22386
                     /locus_tag="ACG8VH_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9109217.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22598..24160
                     /locus_tag="ACG8VH_000635"
     CDS             22598..24160
                     /locus_tag="ACG8VH_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109218.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24153..25286
                     /locus_tag="ACG8VH_000636"
     CDS             24153..25286
                     /locus_tag="ACG8VH_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109219.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25286..26242
                     /locus_tag="ACG8VH_000637"
     CDS             25286..26242
                     /locus_tag="ACG8VH_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109220.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26341..27507
                     /gene="deoB"
                     /locus_tag="ACG8VH_000638"
     CDS             26341..27507
                     /gene="deoB"
                     /locus_tag="ACG8VH_000638"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9109221.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27531..28349
                     /locus_tag="ACG8VH_000639"
     CDS             27531..28349
                     /locus_tag="ACG8VH_000639"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9109222.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28366..29073
                     /gene="deoD"
                     /locus_tag="ACG8VH_000640"
     CDS             28366..29073
                     /gene="deoD"
                     /locus_tag="ACG8VH_000640"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9109223.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29371..30057)
                     /gene="gpmA"
                     /locus_tag="ACG8VH_000641"
     CDS             complement(29371..30057)
                     /gene="gpmA"
                     /locus_tag="ACG8VH_000641"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9109224.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30229..30810)
                     /locus_tag="ACG8VH_000642"
     CDS             complement(30229..30810)
                     /locus_tag="ACG8VH_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9109225.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30943..31626)
                     /gene="rpiA"
                     /locus_tag="ACG8VH_000643"
     CDS             complement(30943..31626)
                     /gene="rpiA"
                     /locus_tag="ACG8VH_000643"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9109226.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31945..32358
                     /gene="rpsL"
                     /locus_tag="ACG8VH_000644"
     CDS             31945..32358
                     /gene="rpsL"
                     /locus_tag="ACG8VH_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9109227.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32442..32912
                     /gene="rpsG"
                     /locus_tag="ACG8VH_000645"
     CDS             32442..32912
                     /gene="rpsG"
                     /locus_tag="ACG8VH_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9109228.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33006..35093
                     /gene="fusA"
                     /locus_tag="ACG8VH_000646"
     CDS             33006..35093
                     /gene="fusA"
                     /locus_tag="ACG8VH_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9109229.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35265..36452
                     /gene="tuf"
                     /locus_tag="ACG8VH_000647"
     CDS             35265..36452
                     /gene="tuf"
                     /locus_tag="ACG8VH_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9109230.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36859..37167
                     /gene="rpsJ"
                     /locus_tag="ACG8VH_000648"
     CDS             36859..37167
                     /gene="rpsJ"
                     /locus_tag="ACG8VH_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9109231.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37198..37827
                     /gene="rplC"
                     /locus_tag="ACG8VH_000649"
     CDS             37198..37827
                     /gene="rplC"
                     /locus_tag="ACG8VH_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9109232.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37855..38478
                     /gene="rplD"
                     /locus_tag="ACG8VH_000650"
     CDS             37855..38478
                     /gene="rplD"
                     /locus_tag="ACG8VH_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9109233.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38478..38768
                     /gene="rplW"
                     /locus_tag="ACG8VH_000651"
     CDS             38478..38768
                     /gene="rplW"
                     /locus_tag="ACG8VH_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9109234.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38807..39640
                     /gene="rplB"
                     /locus_tag="ACG8VH_000652"
     CDS             38807..39640
                     /gene="rplB"
                     /locus_tag="ACG8VH_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9109235.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39683..39961
                     /gene="rpsS"
                     /locus_tag="ACG8VH_000653"
     CDS             39683..39961
                     /gene="rpsS"
                     /locus_tag="ACG8VH_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9109236.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            39984..40331
                     /gene="rplV"
                     /locus_tag="ACG8VH_000654"
     CDS             39984..40331
                     /gene="rplV"
                     /locus_tag="ACG8VH_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9109237.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40345..41001
                     /gene="rpsC"
                     /locus_tag="ACG8VH_000655"
     CDS             40345..41001
                     /gene="rpsC"
                     /locus_tag="ACG8VH_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9109238.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41004..41438
                     /gene="rplP"
                     /locus_tag="ACG8VH_000656"
     CDS             41004..41438
                     /gene="rplP"
                     /locus_tag="ACG8VH_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9109239.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41428..41616
                     /gene="rpmC"
                     /locus_tag="ACG8VH_000657"
     CDS             41428..41616
                     /gene="rpmC"
                     /locus_tag="ACG8VH_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9109240.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41641..41907
                     /gene="rpsQ"
                     /locus_tag="ACG8VH_000658"
     CDS             41641..41907
                     /gene="rpsQ"
                     /locus_tag="ACG8VH_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9109241.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            41965..42333
                     /gene="rplN"
                     /locus_tag="ACG8VH_000659"
     CDS             41965..42333
                     /gene="rplN"
                     /locus_tag="ACG8VH_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9109242.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42370..42678
                     /gene="rplX"
                     /locus_tag="ACG8VH_000660"
     CDS             42370..42678
                     /gene="rplX"
                     /locus_tag="ACG8VH_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9109243.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42706..43245
                     /gene="rplE"
                     /locus_tag="ACG8VH_000661"
     CDS             42706..43245
                     /gene="rplE"
                     /locus_tag="ACG8VH_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9109244.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43264..43449
                     /locus_tag="ACG8VH_000662"
     CDS             43264..43449
                     /locus_tag="ACG8VH_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP9109245.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43485..43883
                     /gene="rpsH"
                     /locus_tag="ACG8VH_000663"
     CDS             43485..43883
                     /gene="rpsH"
                     /locus_tag="ACG8VH_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9109246.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43915..44451
                     /gene="rplF"
                     /locus_tag="ACG8VH_000664"
     CDS             43915..44451
                     /gene="rplF"
                     /locus_tag="ACG8VH_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9109247.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44612..44968
                     /gene="rplR"
                     /locus_tag="ACG8VH_000665"
     CDS             44612..44968
                     /gene="rplR"
                     /locus_tag="ACG8VH_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9109248.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            44989..45489
                     /gene="rpsE"
                     /locus_tag="ACG8VH_000666"
     CDS             44989..45489
                     /gene="rpsE"
                     /locus_tag="ACG8VH_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9109249.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45504..45683
                     /gene="rpmD"
                     /locus_tag="ACG8VH_000667"
     CDS             45504..45683
                     /gene="rpmD"
                     /locus_tag="ACG8VH_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9109250.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45727..46167
                     /gene="rplO"
                     /locus_tag="ACG8VH_000668"
     CDS             45727..46167
                     /gene="rplO"
                     /locus_tag="ACG8VH_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9109251.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46167..47462
                     /gene="secY"
                     /locus_tag="ACG8VH_000669"
     CDS             46167..47462
                     /gene="secY"
                     /locus_tag="ACG8VH_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9109252.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47524..48171
                     /locus_tag="ACG8VH_000670"
     CDS             47524..48171
                     /locus_tag="ACG8VH_000670"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9109253.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48362..48580
                     /gene="infA"
                     /locus_tag="ACG8VH_000671"
     CDS             48362..48580
                     /gene="infA"
                     /locus_tag="ACG8VH_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9109254.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48613..48729
                     /gene="rpmJ"
                     /locus_tag="ACG8VH_000672"
     CDS             48613..48729
                     /gene="rpmJ"
                     /locus_tag="ACG8VH_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9109255.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48747..49112
                     /gene="rpsM"
                     /locus_tag="ACG8VH_000673"
     CDS             48747..49112
                     /gene="rpsM"
                     /locus_tag="ACG8VH_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9109256.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49140..49529
                     /gene="rpsK"
                     /locus_tag="ACG8VH_000674"
     CDS             49140..49529
                     /gene="rpsK"
                     /locus_tag="ACG8VH_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9109257.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49609..50547
                     /locus_tag="ACG8VH_000675"
     CDS             49609..50547
                     /locus_tag="ACG8VH_000675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9109258.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50575..50955
                     /gene="rplQ"
                     /locus_tag="ACG8VH_000676"
     CDS             50575..50955
                     /gene="rplQ"
                     /locus_tag="ACG8VH_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9109259.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51227..52066
                     /locus_tag="ACG8VH_000677"
     CDS             51227..52066
                     /locus_tag="ACG8VH_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9109260.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52042..52911
                     /locus_tag="ACG8VH_000678"
     CDS             52042..52911
                     /locus_tag="ACG8VH_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9109261.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52901..53698
                     /locus_tag="ACG8VH_000679"
     CDS             52901..53698
                     /locus_tag="ACG8VH_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9109262.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        17165 a         9645 c        11597 g        15436 t
ORIGIN      
        1 caaaaagtag gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag
       61 gagaaaaacc atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat
      121 aatatacctg agtagggtat aaattaagac ggaattcctt tctagattga gatattgact
      181 aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag
      241 gggaatatag cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt
      301 agagatgaaa atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac
      361 attggaatgt gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc
      421 gggtcaaaaa taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg
      481 ctagacggtc taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac
      541 ctatcagtcc tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga
      601 ataggaattg aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag
      661 aaaaagaaag tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca
      721 agttataagt ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga
      781 cgagattgac attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga
      841 ataaaatcaa ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc
      901 gcatagacat ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa
      961 aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt
     1021 gggaatccgg tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt
     1081 ttttctgtca aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac
     1141 tttttgatac taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca
     1201 aacgggattg gcatttcatc ttggttcagc tttttttcca attactagga aggagggata
     1261 gattgttcaa gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga
     1321 atctgatgct acttatcttt attgtattat ggttaggtta cataaatcat gattttttga
     1381 tttcaagttt ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga
     1441 aatatttgct gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg
     1501 ggatcttatc gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt
     1561 ccagcagatt cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga
     1621 tgctttctgc catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac
     1681 tttatttttt tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg
     1741 attcacggcc tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc
     1801 ctttagtgat cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca
     1861 gtcacggctt gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc
     1921 tagcagaaaa cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg
     1981 gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt
     2041 ttctgatcac ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg
     2101 ctaatatttt tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact
     2161 tgatttcttt gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag
     2221 gtgttgcgtg ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat
     2281 tagaatagaa ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc
     2341 tctaattcaa ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg
     2401 tggaaaggaa ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg
     2461 gtaactgaat aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc
     2521 tttcttcata gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat
     2581 atggtgtccg gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat
     2641 ttgatgaaga aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa
     2701 gtcgttatag agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa
     2761 tatagtcatg aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa
     2821 gtctatgaag aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt
     2881 tggaaaaaaa tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat
     2941 gcttttacta gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt
     3001 ggtttttgga tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac
     3061 gaaatgacat attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt
     3121 tctcttcata ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa
     3181 agtgccattc gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca
     3241 gatacttggt tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt
     3301 ctttttgcag ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct
     3361 tggatctttg gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag
     3421 cgacaagcaa aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt
     3481 acttcataag tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca
     3541 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa
     3601 taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg
     3661 agttaaacgc ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca
     3721 ataggtaaac tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag
     3781 gtagctaaaa cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg
     3841 atcgactgtt ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat
     3901 cgtaattgtt tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt
     3961 ccatatggaa aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa
     4021 aacacgaaaa cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta
     4081 agaagaagga aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa
     4141 tcggtttttg tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga
     4201 taaattggta gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt
     4261 aactacagaa ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat
     4321 tccctttcaa cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg
     4381 ttcgtttttg aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg
     4441 cgaaatattt catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta
     4501 tcgtttaata agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc
     4561 ttttcttcaa aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta
     4621 aaaagtaacg ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa
     4681 catcaaggaa taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt
     4741 ttagatacag taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc
     4801 tttaataaaa ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt
     4861 cagtcgattg tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga
     4921 agataaaaaa tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt
     4981 ccctctgttt agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga
     5041 taaccttttt cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac
     5101 tccattagct agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca
     5161 gtacgaagtt atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct
     5221 aacaccgatt gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga
     5281 gactgagatc attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg
     5341 gatgtagaca ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta
     5401 gcacaagaaa tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa
     5461 agaaaatatt tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga
     5521 attcaagagt cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc
     5581 gatagaaaaa actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga
     5641 gtttttggat tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca
     5701 catattatgt tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt
     5761 ataaagagta tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac
     5821 ttctatataa attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat
     5881 tgattctata atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca
     5941 gagaacgggt tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg
     6001 ggaaaacgaa gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc
     6061 cgataggata atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca
     6121 gtcattaaat gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg
     6181 gattagtgac tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt
     6241 acgcatgata aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga
     6301 tacttaccta gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt
     6361 tatttcctct ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga
     6421 ggaactagtg gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag
     6481 tactcctgat gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt
     6541 taattataga tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca
     6601 ctgctatatt tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt
     6661 ttataaaagg aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag
     6721 aattaacgga tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc
     6781 catctttatt tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg
     6841 caataattgt tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga
     6901 taacaagaca atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg
     6961 gttgcggttt cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc
     7021 gggctaaatc gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa
     7081 tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca
     7141 actgaagctc gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg
     7201 acaatacggc gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg
     7261 tattcgtccg accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat
     7321 gatcatgtat catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc
     7381 cgttgaaatc aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga
     7441 atcagcaatt tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg
     7501 gcgatccgta atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg
     7561 attgaatttt ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg
     7621 ctgtgcttct ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga
     7681 actgataaat gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac
     7741 aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc
     7801 ttgagcaaga atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag
     7861 taaatgcgtc gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc
     7921 gtgaatcaag gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc
     7981 tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg
     8041 gacacgcagc agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc
     8101 agaaacatct aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat
     8161 ttctacgata gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat
     8221 aatctatcat ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt
     8281 ggtttttatg tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt
     8341 gtttgttttt gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg
     8401 aaaactgatt atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt
     8461 ttggcggagc cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg
     8521 ctgttgcttg ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg
     8581 atttttatca tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc
     8641 taagattatc cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag
     8701 aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt
     8761 ttgtgacgtt gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga
     8821 atcgagaaac aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag
     8881 aagtcaaggt gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt
     8941 taggtgtttt cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata
     9001 atatgatggt tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg
     9061 aaattggtgt agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata
     9121 agcatgctgc ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt
     9181 taggctatta ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg
     9241 ccagctatca ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg
     9301 actacttagg gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg
     9361 caatccatca taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg
     9421 gagaacgtat ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag
     9481 aaggtctata tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct
     9541 atatcactga gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc
     9601 agccgagaat cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg
     9661 gggtaaatgt gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg
     9721 ggtataacaa aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa
     9781 aagaatcagc ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga
     9841 taatggagga agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg
     9901 catattcagg cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa
     9961 actttgaaca tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata
    10021 attcgcaaac aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca
    10081 tttttatcca tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag
    10141 actttattgt actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca
    10201 ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag
    10261 gagccgttcg tgacggattt ttaacagcga tgccagctat tttattttca agcattttca
    10321 ttttgattgc tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat
    10381 ggctatggaa agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca
    10441 ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca
    10501 ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg
    10561 aaggtggatt ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg
    10621 catttttaac agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc
    10681 ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt
    10741 cagtatttgc ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg
    10801 cagaagcaat cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa
    10861 tcgctattat ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga
    10921 tcgtggagcc ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt
    10981 tcaattccgg tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa
    11041 ctatgggagg aactggggca acattggttg ttccttttat cttcctactc tttgctaagt
    11101 ctaagcagtt gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg
    11161 aaccattgct atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt
    11221 taacacctgt tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca
    11281 gctttatgta cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg
    11341 gcatgggtat cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct
    11401 atctaccatt ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag
    11461 aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag
    11521 cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg
    11581 cagggacaag tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac
    11641 cgatcagtgc aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata
    11701 tggtggtctt agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc
    11761 gcttaggaat caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc
    11821 ctgaaaaagc tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa
    11881 atgggaattt ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct
    11941 gtttcaaaaa ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc
    12001 ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc
    12061 ttagagattt taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct
    12121 tataatagta atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac
    12181 cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat
    12241 ttttgggttg atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa
    12301 gaaatgaata cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg
    12361 gacgtttatc ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct
    12421 tatggcaagt cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag
    12481 tctggtatcc aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta
    12541 gctgatggtg gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt
    12601 gtgtcatttg atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa
    12661 cttcaaacca atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa
    12721 acagcttatg ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc
    12781 gaagcagctg cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat
    12841 cttgccgatc ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa
    12901 cctctttggt acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt
    12961 gttcaatcca ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac
    13021 gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat
    13081 gcaagtttcg aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa
    13141 caaggaaccg ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa
    13201 ctaacttttt ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata
    13261 agcaaaggaa cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat
    13321 cagttatata tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt
    13381 gaatggacaa aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg
    13441 gtttatagca aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa
    13501 caatagactg cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc
    13561 gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc
    13621 cagtcatttt taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta
    13681 aagagagttc aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa
    13741 cataaagttt gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac
    13801 ttagatggat agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg
    13861 ataagaatga aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt
    13921 ttaatgaaaa tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc
    13981 atattatgtt ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat
    14041 acacgatacg gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga
    14101 cttcccatgg gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga
    14161 taccgactgt gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg
    14221 gaagaacagt gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac
    14281 gcaagatagc atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa
    14341 cacgaatacc aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc
    14401 ctaactgagt ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca
    14461 attcaaattg catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg
    14521 ctgtttctgt accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga
    14581 cgccttcttc accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc
    14641 caatgatttt acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat
    14701 ctcttgcatt gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt
    14761 gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac
    14821 cgatcgcttt ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa
    14881 gcaagacttt tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt
    14941 tatccattgt ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt
    15001 cgattccgta ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg
    15061 ttgtgtcttg gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt
    15121 caggaacttt agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt
    15181 ctttaaacaa gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt
    15241 ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa
    15301 tccctaatac aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat
    15361 catatacact gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt
    15421 cttctgcagt aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc
    15481 ttgtgatcaa ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg
    15541 tcatatcggc aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat
    15601 agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt
    15661 tcagttttat ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa
    15721 tttttcaaat tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct
    15781 ccgatagcaa aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca
    15841 ccaaaacgat ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa
    15901 aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc
    15961 ccctcaacga tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca
    16021 tacacatcat gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc
    16081 aagaaatcga tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt
    16141 ccacgctctt ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg
    16201 tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt
    16261 ccaacggcta cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga
    16321 agaaataaac ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga
    16381 tgagcaagag gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt
    16441 ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc
    16501 cattcttctc ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt
    16561 ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga
    16621 ttgccgatct ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg
    16681 cgcacggata cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca
    16741 tgacaaatca ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt
    16801 ttgagttaaa aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa
    16861 cagtggatta tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg
    16921 aaggaaagat cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg
    16981 cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag
    17041 gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag
    17101 cagttcatga agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa
    17161 aagttgtaca tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga
    17221 caattgtgat ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat
    17281 ttggtaatgc agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag
    17341 aataactagc tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg
    17401 tacgtatcag ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca
    17461 aaaagaaata gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac
    17521 ttgtatagaa ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc
    17581 cttttttctc tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag
    17641 ggagccaata ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta
    17701 acaactgtct cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa
    17761 aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg
    17821 atcaatcgac attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc
    17881 tccaaactga tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta
    17941 tgcaattctc gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc
    18001 tcagtggacg gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac
    18061 agcggttttt atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat
    18121 aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt
    18181 gaaaatggcc atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa
    18241 gaacgtttca acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa
    18301 tttgttgaac atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc
    18361 aaacataaag atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat
    18421 caggcgatcg atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat
    18481 ggattaaagc tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt
    18541 gatattggat tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa
    18601 gttccgaaca aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca
    18661 aagggctaac tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt
    18721 ttttattttt agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa
    18781 taccgaatga agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga
    18841 gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg
    18901 gaaagattcc agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga
    18961 caaatgaaga aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt
    19021 tgagttctct tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga
    19081 ccacattgat tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg
    19141 gacgcggtct aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc
    19201 agatcgagct ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa
    19261 ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca
    19321 cagcgaccgt tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg
    19381 caggagcaga tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca
    19441 tcgaagatgc aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag
    19501 aaacagtggc ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc
    19561 tagaagttgt cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa
    19621 tgtgctacgc cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg
    19681 aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag
    19741 aaatgatcca aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga
    19801 ctgcacgcta cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg
    19861 caaatgaatt aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag
    19921 atattgatca cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag
    19981 gtgagtcact attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat
    20041 tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca
    20101 tcacagaata aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg
    20161 aactaaaccg aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga
    20221 tgcaaattat cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat
    20281 gggttgcgct agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg
    20341 ggtttcctct aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca
    20401 ataacggtgc agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg
    20461 atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg
    20521 tcaaagttat tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa
    20581 tagcaaaagc tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag
    20641 caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag
    20701 cttccggcgg tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa
    20761 gaatcggaac aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat
    20821 tttttcaaag gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga
    20881 agcgttagat aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac
    20941 ggaatcaggc aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa
    21001 ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca
    21061 tctagtcgta gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt
    21121 aatggcagaa ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac
    21181 gaaggcaacg accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact
    21241 catgttcatt cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca
    21301 gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt
    21361 ttaggtgcat gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac
    21421 acaacaactg cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag
    21481 tcagcttggg aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat
    21541 ggcttccaat atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca
    21601 ttgaatgctg gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa
    21661 gaacaagcaa caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga
    21721 ttagataacg ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt
    21781 gcagcagctt atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa
    21841 gtaatcgatc gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta
    21901 ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga
    21961 cgttcaattg ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc
    22021 ggtacaggta acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa
    22081 gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc
    22141 gaaaagaaaa acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat
    22201 ttggcacaaa aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg
    22261 aaagaagacg gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt
    22321 gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa
    22381 aactaaaacc atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta
    22441 gaacaagaat tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc
    22501 cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg
    22561 ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat
    22621 agaaatgcag aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt
    22681 acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac
    22741 attgatgaac gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg
    22801 gaaagaagta aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca
    22861 tcagcacttt atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga
    22921 gcctagtcgc gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc
    22981 tgaacagtat ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat
    23041 ggaacaacgg gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga
    23101 tgagccgaca gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga
    23161 attagtcaaa gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc
    23221 agtagctgac cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa
    23281 agacgtaacc tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac
    23341 gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa
    23401 agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt
    23461 acttggtatc gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg
    23521 tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa
    23581 accccgaaaa atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg
    23641 gttagtccta gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc
    23701 ttacagtaaa aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat
    23761 cgaagaatat gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg
    23821 aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt
    23881 tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat
    23941 cgaacaaagg gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt
    24001 aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc
    24061 gaaagaaaca tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc
    24121 acgaaaagag ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta
    24181 atttattagt gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg
    24241 ctgcttttgg ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt
    24301 cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag
    24361 gtttttctgt agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt
    24421 gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt
    24481 tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag
    24541 gtttacttcg agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata
    24601 tcttattata cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa
    24661 gtttggactc ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac
    24721 aagcttttgg cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt
    24781 tcgtttggct gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga
    24841 atccttttgc ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg
    24901 tcatttctgg cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa
    24961 acttctttgt tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc
    25021 ttggttctgg atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga
    25081 tcggtggact tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga
    25141 ttgctttgat tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag
    25201 tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc
    25261 aaaaccatga agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac
    25321 ttctacactg gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga
    25381 acgaagcggg atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc
    25441 catcgttttc aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat
    25501 tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag
    25561 tttgcgtgct gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg
    25621 tgttttcttg atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct
    25681 ggggtatttt tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa
    25741 aaatacattc ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat
    25801 cttcaagacg aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga
    25861 cacattaggt atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt
    25921 aggcggtatg ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac
    25981 gatcgttgga caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt
    26041 aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc
    26101 acaattacca gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct
    26161 gacaatcatt gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa
    26221 gaactatatc aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt
    26281 ttttcgtctc gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc
    26341 atgtttaaac gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat
    26401 gccgataagt tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga
    26461 ttgaacgttc cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg
    26521 gaatcagtga aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa
    26581 gatacgatga caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg
    26641 tttcctaatg gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa
    26701 gtcgtatgta acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa
    26761 atgaaaacag gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca
    26821 catgaagaaa tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata
    26881 acaaaagatg atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca
    26941 ggagacttca cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca
    27001 gtccttgatt ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat
    27061 attttcaacg gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt
    27121 gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta
    27181 gattttgatg cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa
    27241 gaatttgact tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg
    27301 attacagccg atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat
    27361 gtaccgttat tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat
    27421 tattcagata tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc
    27481 gaaagcttct tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt
    27541 taagcgagat gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg
    27601 attttggttt gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg
    27661 taatgatttc ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag
    27721 gaaagctagt ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc
    27781 actactatga aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac
    27841 taggggccca ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac
    27901 caggagattt gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg
    27961 gtccgaatga agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg
    28021 actatcaagc aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg
    28081 tatatatggg ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga
    28141 caataggagc cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca
    28201 gcggattaaa agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga
    28261 atctgagcca tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat
    28321 tggttaaaga aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt
    28381 gaagcgaaag aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct
    28441 aaatatattg cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg
    28501 ttaggcttta ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga
    28561 atgccatcag ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg
    28621 attcgagtag gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta
    28681 gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt
    28741 cctccaatca gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc
    28801 tatacggttc atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca
    28861 gagacgttcc aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt
    28921 tattacttag gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat
    28981 ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc
    29041 gaagtaggat tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat
    29101 taagtaataa gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta
    29161 ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa
    29221 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
    29281 ttgtgaccga gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat
    29341 cgtgtcacag tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa
    29401 gtcatcattt aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc
    29461 ttcgtcagag ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc
    29521 tactaaaaca gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa
    29581 agcgcgttct aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat
    29641 tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag
    29701 agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc
    29761 ttttttgttc aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat
    29821 ttgtggtacc caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg
    29881 agttaaaaca gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc
    29941 gccttctttt gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa
    30001 ttggttcaat gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt
    30061 tagctctcct tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca
    30121 attttttgga agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga
    30181 gaatataaaa aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa
    30241 ttgctcgatt tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc
    30301 gcattttgaa gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt
    30361 tttgatcgtt tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc
    30421 atctaatcca actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc
    30481 cattaattgg aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat
    30541 caagtgcagc ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt
    30601 gcctaatcga tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac
    30661 tgactccaaa tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat
    30721 ttcttgacaa ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat
    30781 ggttgtgcgt gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa
    30841 ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat
    30901 tttccgctgt ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc
    30961 gtcaagaatc tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa
    31021 aagcccatgt tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg
    31081 ttcgatgact tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc
    31141 tccgttttcg tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag
    31201 ctgttgggag ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac
    31261 taatttagaa gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc
    31321 gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc
    31381 accgtctatt gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa
    31441 tttttccgct tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc
    31501 ttcacgcata cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag
    31561 acctacgatc atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa
    31621 attcatcttt gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc
    31681 gtgatttacg gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa
    31741 ttgcatctct tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta
    31801 caggtttctg tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga
    31861 cattgcattt attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa
    31921 atcgtgggaa ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt
    31981 aaatcaaagg tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag
    32041 aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca
    32101 atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac
    32161 ttaattgaag ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt
    32221 gtattattac gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc
    32281 ggtgctttag atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact
    32341 aaaaaaccta aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga
    32401 agtaattact atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg
    32461 ttgcaaaacg tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga
    32521 tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct
    32581 ttgacttgat caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga
    32641 aaaacgttca acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag
    32701 taccagttga agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact
    32761 acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg
    32821 ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg
    32881 ccaaccgtgc gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata
    32941 agtcgacctc agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa
    33001 ggagaatgac cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca
    33061 tcgatgctgg taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata
    33121 aaattggtga aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac
    33181 gtggtattac gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca
    33241 tcatcgacac accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac
    33301 ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag
    33361 tttggcgtca agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca
    33421 agatcggtgc tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg
    33481 ctcacccaat ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg
    33541 taacaatgaa ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa
    33601 ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag
    33661 ctgaaactga tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg
    33721 agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg
    33781 gttcagcatt taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc
    33841 catcaccgtt agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag
    33901 aacgtcctgc agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc
    33961 cattcgtagg tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat
    34021 acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg
    34081 ccaactctcg ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt
    34141 tgaaagacac aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat
    34201 ccatggaatt cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc
    34261 aagataaaat gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg
    34321 aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata
    34381 tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag
    34441 tatcttaccg tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt
    34501 ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg
    34561 ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag
    34621 ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag
    34681 tagatatcaa agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg
    34741 ccttccgtgt tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa
    34801 tccttgaacc aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta
    34861 tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc
    34921 aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc
    34981 gttctgctac tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac
    35041 caaaatcagt acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg
    35101 aaaccggata gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt
    35161 gtaaaataat tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc
    35221 agacaaataa aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat
    35281 ttgaccgttc taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa
    35341 ctacattaac agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg
    35401 cttacgatca aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag
    35461 ctcacgttga gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg
    35521 cggattacgt taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag
    35581 tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag
    35641 ttggtgttcc ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat
    35701 tactagaatt agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg
    35761 acgatgttcc tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg
    35821 aagaaaaaat tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg
    35881 acaacgacaa accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta
    35941 ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg
    36001 ttggtattgc tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat
    36061 tgttagacta cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg
    36121 aagacatcca acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat
    36181 tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca
    36241 ctaactaccg tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac
    36301 cagaaggaac tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc
    36361 acccaatcgc tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg
    36421 gtgccggtgt cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca
    36481 tttattcgga cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta
    36541 tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa
    36601 agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc
    36661 cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc
    36721 acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac
    36781 gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata
    36841 ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa
    36901 caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct
    36961 agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg
    37021 actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc
    37081 gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct
    37141 ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg
    37201 accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa
    37261 ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg
    37321 gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca
    37381 aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt
    37441 gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt
    37501 ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg
    37561 gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt
    37621 cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct
    37681 ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca
    37741 gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact
    37801 atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg
    37861 aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa
    37921 atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg
    37981 tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt
    38041 aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa
    38101 tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct
    38161 aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac
    38221 ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt
    38281 cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt
    38341 gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt
    38401 gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt
    38461 gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa
    38521 aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca
    38581 aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca
    38641 tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac
    38701 gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg
    38761 ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa
    38821 gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac
    38881 aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa
    38941 caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat
    39001 cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc
    39061 aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt
    39121 agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa
    39181 agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga
    39241 aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact
    39301 tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt
    39361 agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat
    39421 cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat
    39481 gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc
    39541 accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa
    39601 atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc
    39661 acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga
    39721 gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac
    39781 ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga
    39841 tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga
    39901 atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta
    39961 atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa
    40021 aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa
    40081 gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg
    40141 aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa
    40201 acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc
    40261 gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat
    40321 cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta
    40381 ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta
    40441 cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca
    40501 acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca
    40561 ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa
    40621 ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca
    40681 aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct
    40741 caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta
    40801 tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt
    40861 cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac
    40921 ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac
    40981 actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat
    41041 tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg
    41101 gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg
    41161 caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat
    41221 cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat
    41281 gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag
    41341 tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa
    41401 aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact
    41461 gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa
    41521 ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca
    41581 cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt
    41641 atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg
    41701 gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc
    41761 atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac
    41821 atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa
    41881 gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca
    41941 aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat
    42001 aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact
    42061 gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc
    42121 aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca
    42181 gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc
    42241 cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg
    42301 aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc
    42361 gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa
    42421 aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt
    42481 gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt
    42541 gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct
    42601 ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt
    42661 gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa
    42721 gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt
    42781 atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca
    42841 aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca
    42901 atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt
    42961 ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct
    43021 gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt
    43081 ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta
    43141 gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa
    43201 tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac
    43261 tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa
    43321 gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt
    43381 tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct
    43441 agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc
    43501 caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag
    43561 aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg
    43621 ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat
    43681 ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc
    43741 gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca
    43801 tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg
    43861 aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc
    43921 cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac
    43981 aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa
    44041 atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa
    44101 acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt
    44161 ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa
    44221 cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact
    44281 gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc
    44341 gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt
    44401 cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga
    44461 aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa
    44521 aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata
    44581 gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac
    44641 acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg
    44701 cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg
    44761 tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga
    44821 agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt
    44881 agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc
    44941 tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc
    45001 gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt
    45061 gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga
    45121 cacgtaggat ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt
    45181 gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa
    45241 gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg
    45301 gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact
    45361 tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg
    45421 aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta
    45481 atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta
    45541 tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca
    45601 gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc
    45661 tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc
    45721 aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt
    45781 gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa
    45841 aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc
    45901 cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat
    45961 cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa
    46021 gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac
    46081 aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg
    46141 gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa
    46201 aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg
    46261 cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat
    46321 ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa
    46381 tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg
    46441 tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag
    46501 cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat
    46561 tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga
    46621 tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag
    46681 aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc
    46741 ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat
    46801 ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg
    46861 taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg
    46921 gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta
    46981 tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat
    47041 tcggttctga aaactggtac aaagtgatga tggagatctt taactataat acagcacctg
    47101 gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag
    47161 taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac
    47221 gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg
    47281 gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc
    47341 ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag
    47401 aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt
    47461 aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa
    47521 acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa
    47581 atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg
    47641 caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt
    47701 cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa
    47761 ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg
    47821 ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc
    47881 ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa
    47941 attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat
    48001 caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat
    48061 agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct
    48121 cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc
    48181 ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata
    48241 tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga
    48301 actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg
    48361 cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc
    48421 aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat
    48481 tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata
    48541 cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca
    48601 ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta
    48661 attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt
    48721 caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg
    48781 ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa
    48841 agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac
    48901 aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg
    48961 aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc
    49021 gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc
    49081 cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca
    49141 tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg
    49201 cacatatcca ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg
    49261 cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt
    49321 ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga
    49381 ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac
    49441 aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg
    49501 cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta
    49561 acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc
    49621 gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa
    49681 cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct
    49741 tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct
    49801 accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg
    49861 ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca
    49921 gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac
    49981 atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt
    50041 ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc
    50101 gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga
    50161 caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg
    50221 ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac
    50281 ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa
    50341 aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg
    50401 aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc
    50461 aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta
    50521 ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt
    50581 tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact
    50641 gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca
    50701 actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct
    50761 gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt
    50821 gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc
    50881 ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc
    50941 ttagaacttg tctaaaatca cactaataac atcactttat agatgattct tttagagtgt
    51001 tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg
    51061 cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac
    51121 agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt
    51181 tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat
    51241 tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga
    51301 tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg
    51361 aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa
    51421 agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat
    51481 ggtttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt
    51541 tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact
    51601 tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca
    51661 aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga
    51721 tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa
    51781 gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc
    51841 taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa
    51901 agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga
    51961 aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga
    52021 agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag
    52081 ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac
    52141 agttatactg cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg
    52201 aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg
    52261 gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt
    52321 ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac
    52381 tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg
    52441 ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga
    52501 gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg
    52561 gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag
    52621 gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc caattttgca
    52681 gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc
    52741 tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt
    52801 gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa
    52861 ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt
    52921 tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt
    52981 agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt
    53041 gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat
    53101 ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt
    53161 tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt
    53221 gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact
    53281 cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt
    53341 gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt
    53401 tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt
    53461 tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc
    53521 tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg
    53581 ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac
    53641 gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac
    53701 gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc
    53761 agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt
    53821 atctgaaaag aaagaacgaa aaa
//
LOCUS       DAZKCC010000011        50083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000011 DAZKCC010000000
VERSION     DAZKCC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.011"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..273)
                     /locus_tag="ACG8VH_000680"
     CDS             complement(<1..273)
                     /locus_tag="ACG8VH_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9109263.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGING"
     gene            complement(275..472)
                     /locus_tag="ACG8VH_000681"
     CDS             complement(275..472)
                     /locus_tag="ACG8VH_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9109264.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(866..2593)
                     /locus_tag="ACG8VH_000682"
     CDS             complement(866..2593)
                     /locus_tag="ACG8VH_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP9109265.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2586..3779)
                     /locus_tag="ACG8VH_000683"
     CDS             complement(2586..3779)
                     /locus_tag="ACG8VH_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9109266.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3966..4610)
                     /locus_tag="ACG8VH_000684"
     CDS             complement(3966..4610)
                     /locus_tag="ACG8VH_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9109267.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4704..4838)
                     /locus_tag="ACG8VH_000685"
     CDS             complement(4704..4838)
                     /locus_tag="ACG8VH_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP9109268.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4840..6972)
                     /gene="feoB"
                     /locus_tag="ACG8VH_000686"
     CDS             complement(4840..6972)
                     /gene="feoB"
                     /locus_tag="ACG8VH_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP9109269.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6969..7442)
                     /locus_tag="ACG8VH_000687"
     CDS             complement(6969..7442)
                     /locus_tag="ACG8VH_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP9109270.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7843..8067
                     /gene="nrdH"
                     /locus_tag="ACG8VH_000688"
     CDS             7843..8067
                     /gene="nrdH"
                     /locus_tag="ACG8VH_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9109271.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8226..10385
                     /gene="nrdE"
                     /locus_tag="ACG8VH_000689"
     CDS             8226..10385
                     /gene="nrdE"
                     /locus_tag="ACG8VH_000689"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9109272.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10435..11400
                     /gene="nrdF"
                     /locus_tag="ACG8VH_000690"
     CDS             10435..11400
                     /gene="nrdF"
                     /locus_tag="ACG8VH_000690"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9109273.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11489..12628)
                     /locus_tag="ACG8VH_000691"
     CDS             complement(11489..12628)
                     /locus_tag="ACG8VH_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9109274.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12937..13650)
                     /locus_tag="ACG8VH_000692"
     CDS             complement(12937..13650)
                     /locus_tag="ACG8VH_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9109275.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13904..14605)
                     /gene="nagB"
                     /locus_tag="ACG8VH_000693"
     CDS             complement(13904..14605)
                     /gene="nagB"
                     /locus_tag="ACG8VH_000693"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9109276.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14839..16371
                     /locus_tag="ACG8VH_000694"
     CDS             14839..16371
                     /locus_tag="ACG8VH_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9109277.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16562..17764)
                     /locus_tag="ACG8VH_000695"
     CDS             complement(16562..17764)
                     /locus_tag="ACG8VH_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9109278.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17846..18535)
                     /locus_tag="ACG8VH_000696"
     CDS             complement(17846..18535)
                     /locus_tag="ACG8VH_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9109279.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18641..19009)
                     /gene="rplL"
                     /locus_tag="ACG8VH_000697"
     CDS             complement(18641..19009)
                     /gene="rplL"
                     /locus_tag="ACG8VH_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9109280.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19062..19565)
                     /gene="rplJ"
                     /locus_tag="ACG8VH_000698"
     CDS             complement(19062..19565)
                     /gene="rplJ"
                     /locus_tag="ACG8VH_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9109281.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19769..19896)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19975..20664)
                     /gene="rplA"
                     /locus_tag="ACG8VH_000699"
     CDS             complement(19975..20664)
                     /gene="rplA"
                     /locus_tag="ACG8VH_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9109282.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20768..21190)
                     /gene="rplK"
                     /locus_tag="ACG8VH_000700"
     CDS             complement(20768..21190)
                     /gene="rplK"
                     /locus_tag="ACG8VH_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9109283.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21533..22201
                     /gene="sdaAB"
                     /locus_tag="ACG8VH_000701"
     CDS             21533..22201
                     /gene="sdaAB"
                     /locus_tag="ACG8VH_000701"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP9109284.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22214..23086
                     /gene="sdaAA"
                     /locus_tag="ACG8VH_000702"
     CDS             22214..23086
                     /gene="sdaAA"
                     /locus_tag="ACG8VH_000702"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP9109285.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23177..24277)
                     /locus_tag="ACG8VH_000703"
                     /pseudo
     CDS             complement(23177..24277)
                     /locus_tag="ACG8VH_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24369..25256)
                     /locus_tag="ACG8VH_000704"
     CDS             complement(24369..25256)
                     /locus_tag="ACG8VH_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9109286.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25333..25878)
                     /gene="nusG"
                     /locus_tag="ACG8VH_000705"
     CDS             complement(25333..25878)
                     /gene="nusG"
                     /locus_tag="ACG8VH_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9109287.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25979..26149)
                     /gene="secE"
                     /locus_tag="ACG8VH_000706"
     CDS             complement(25979..26149)
                     /gene="secE"
                     /locus_tag="ACG8VH_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9109288.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26173..26325)
                     /gene="rpmG"
                     /locus_tag="ACG8VH_000707"
     CDS             complement(26173..26325)
                     /gene="rpmG"
                     /locus_tag="ACG8VH_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9109289.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26487..27131
                     /gene="cbpA"
                     /locus_tag="ACG8VH_000708"
     CDS             26487..27131
                     /gene="cbpA"
                     /locus_tag="ACG8VH_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP9109290.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27187..27690)
                     /locus_tag="ACG8VH_000709"
     CDS             complement(27187..27690)
                     /locus_tag="ACG8VH_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9109291.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27703..27749)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28287..29252
                     /locus_tag="ACG8VH_000710"
     CDS             28287..29252
                     /locus_tag="ACG8VH_000710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9109292.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29351..30523)
                     /locus_tag="ACG8VH_000711"
     CDS             complement(29351..30523)
                     /locus_tag="ACG8VH_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109293.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30510..31655)
                     /locus_tag="ACG8VH_000712"
     CDS             complement(30510..31655)
                     /locus_tag="ACG8VH_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109294.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31669..32593)
                     /locus_tag="ACG8VH_000713"
                     /pseudo
     CDS             complement(31669..32593)
                     /locus_tag="ACG8VH_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32710..33789
                     /locus_tag="ACG8VH_000714"
     CDS             32710..33789
                     /locus_tag="ACG8VH_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9109295.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33786..34414
                     /locus_tag="ACG8VH_000715"
                     /pseudo
     CDS             33786..34414
                     /locus_tag="ACG8VH_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34524..34751)
                     /locus_tag="ACG8VH_000716"
     CDS             complement(34524..34751)
                     /locus_tag="ACG8VH_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9109296.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34854..37442)
                     /locus_tag="ACG8VH_000717"
     CDS             complement(34854..37442)
                     /locus_tag="ACG8VH_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP9109297.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37462..38121)
                     /locus_tag="ACG8VH_000718"
     CDS             complement(37462..38121)
                     /locus_tag="ACG8VH_000718"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9109298.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38170..38673)
                     /gene="trmL"
                     /locus_tag="ACG8VH_000719"
     CDS             complement(38170..38673)
                     /gene="trmL"
                     /locus_tag="ACG8VH_000719"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9109299.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38894..39760)
                     /locus_tag="ACG8VH_000720"
     CDS             complement(38894..39760)
                     /locus_tag="ACG8VH_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9109300.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39869..40510
                     /locus_tag="ACG8VH_000721"
     CDS             39869..40510
                     /locus_tag="ACG8VH_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9109301.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40572..41933)
                     /gene="mgtE"
                     /locus_tag="ACG8VH_000722"
     CDS             complement(40572..41933)
                     /gene="mgtE"
                     /locus_tag="ACG8VH_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP9109302.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41951..42850)
                     /locus_tag="ACG8VH_000723"
     CDS             complement(41951..42850)
                     /locus_tag="ACG8VH_000723"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9109303.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42852..43649)
                     /locus_tag="ACG8VH_000724"
     CDS             complement(42852..43649)
                     /locus_tag="ACG8VH_000724"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP9109304.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43627..44331)
                     /locus_tag="ACG8VH_000725"
     CDS             complement(43627..44331)
                     /locus_tag="ACG8VH_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP9109305.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44462..45038
                     /locus_tag="ACG8VH_000726"
                     /pseudo
     CDS             44462..45038
                     /locus_tag="ACG8VH_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45156..45833
                     /locus_tag="ACG8VH_000727"
     CDS             45156..45833
                     /locus_tag="ACG8VH_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP9109306.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45912..47720)
                     /gene="pepF"
                     /locus_tag="ACG8VH_000728"
     CDS             complement(45912..47720)
                     /gene="pepF"
                     /locus_tag="ACG8VH_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP9109307.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47896..48894)
                     /locus_tag="ACG8VH_000729"
     CDS             complement(47896..48894)
                     /locus_tag="ACG8VH_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP9109308.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49120..49773)
                     /locus_tag="ACG8VH_000730"
     CDS             complement(49120..49773)
                     /locus_tag="ACG8VH_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP9109309.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14995 a        10506 c         8779 g        15803 t
ORIGIN      
        1 accatttatt ccagcagttc ttccaatttc actcaaattt cttattaatt cctcttgata
       61 ttcagtgaat cttattttat tagtataagc agtaaaatat agataattca ccaatctatt
      121 tattaaagga ccgggatcta attcatattt ttgtttttct aattttaaat ataccttttg
      181 taaaacctca agaatatcag aatatttagg atctttactt atagatatat cattatataa
      241 ttcatgaaca atttgcttag catttttttt cattttagca cttccctgga attgctccac
      301 ctaaaaaacc acctatagac attcctgcaa tacaagtagt tgccttggcc caatcgaccg
      361 tacatttatt tttagtgcaa tacactccat ttccataata ttttccacta tgagtggtac
      421 ccccatagat aagttgtgtc tctttaatag acaaaatttt taaatgtttc ataatctaat
      481 cacccctaaa ttaatgtttt tagactatta ttctaacatt cattaaattt caaaaaacaa
      541 ttccttcttg aaacgtagca aaataattcc tgaataagaa tttaatcact tttaagtata
      601 taaactatgt atattcttta atattttttg agaatgcact tcgtacataa taaactttaa
      661 tcacattagt ctttcatacc tacgatcgat ctctttgact cgcacccata aattcataat
      721 agtttgtcgg cgtctttgga cgtttcgaag atgagctacg cattctcttt ttttatagac
      781 aaaaaagata gagacgaaca tggagcttcc aaatttggat atttgatcca attttggaag
      841 ctccatttat ttcgtgtctg tctttttatt ttttccagct ttcgactaat tcttggttct
      901 ctttgatcca gtcttttgct gcttgctgtg gatcttttcc attgttgatt tccaacataa
      961 ctttttccat atctttttct gaccaatgga aattgtctaa tactttgtaa gcttctggct
     1021 ggtcttcttt caatcctttt cgaaccatcg tatggatctg ctcttcgctt cccattccgt
     1081 tttcaggatc ttctaagtat ttcagatcat atttggcaaa catccaatga ggtgtccatc
     1141 ctgtaactac aattggttcg tgttttttga tcgcttgacc tagtgcaacg gtcattgctc
     1201 ctgaagaaga tgtctctact ttccagtctt tcaagttgtc atatttttgg atcgtgtttt
     1261 cagcagccgt aactacacca gctcctggct cgattcctat gattttctta cctgcctgat
     1321 cggttaactc atcgatcgaa tttacatcca tgtaggctgg cactgctaaa ccaacttttg
     1381 ctcctgtcaa attggcacct aggtcttcga cttgatcttt atattgtgca tattgtgaac
     1441 catgagtttt aggtagccaa gctgaaacca tggcatctga ctcgccattt gaaacagatt
     1501 tccacatgat tgaattatct aatggcgtca tcgttacatc atagcccatt tgtttcaata
     1561 cttcaccgac tacatttgtt gaagcaactt ctgtatccca ttcaacataa gataagttga
     1621 tacgttttgt ttctttggca gatgaaaaac tccctatacc aatcgcacct aagatcacta
     1681 ctactacagc accaatgatc agtccttgac gttttttact tttctgtgtt gttgctcctg
     1741 cagctttttt attgtttggc tggttcaaat gctgtgtaaa acggtcaatg atgattgcta
     1801 agataactaa tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg
     1861 ataatacgcc gcgtcctaag ccgggtgcac cgatcataga ggcaataacg accattgaaa
     1921 gtgacagcat tgttgtttga ttgatccctg ccatgatcgt acttttagct aaaggcaatt
     1981 ctagtttgaa tagtttttga cgacttgtgc tcccgaatga atcagaagct tctactaatt
     2041 ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc agagcaaaga
     2101 tgactgaggc aaatacccct ggaaccatcc cgataccgaa aaatgcaact gctggaatca
     2161 aataaacgaa tcctggcatt gtctgcataa agtcaaggat cggtttgata atactttggg
     2221 ctttactgct ttttgccatc aagatcccta atggtacccc aatgatgatc gagatcacac
     2281 tagataaaag aacaagtgtg acagtattca tcaaatcatt ccatagacct tggttataaa
     2341 taaaccatag accgattaat gtgaacaacg taagtcctgg acgtttctta gaaataaaga
     2401 aagctgctac cgtcaataac aagataaaca aaggagctgg aatcactaac agtaaatttg
     2461 taatccccga catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag
     2521 ttgtcgtaaa ccattctgtg atattttcta cccagtttgc cactggtaat tgatagttca
     2581 atagattatt catgttcact cacctcgctt gtttctgcta atgcttcaat cacactacct
     2641 cggatgacga caccttttaa ttttccatcc tctacaactg caagtggagt cggtgagtca
     2701 taaataatat tgaaaatatc acttaccacc atgtctttcc caatcaccgt cacattttca
     2761 tctaggaagt cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg atctgcagtg
     2821 atgatccctt tcaggtgtct ctttctgtca acagccatca gcatgctgac ttcttcatta
     2881 cgcatccttg tcaatgcaac gtttggtcca tcagattcga tattcgtcgt caatgcaggt
     2941 accatgatgt tttgagccgt aagcactttg gaacgatcga cttcttccac aaattcacgt
     3001 acataatcat ttgctggatt ggtcaatatt tcttcacctg ttccgatctg catgatttcg
     3061 ccgtctttca tcaaggcgat tctgtcccca atccgtaaag cttcattcaa atcatgggta
     3121 atgaaaataa tcgttttttg aacattggct tgcaggtcca gtaattcgtc ttgcatttct
     3181 cgccggatca gcggatcaag agccgagaac gcttcgtcca tcagcaagat ctcaggatca
     3241 tttgctaaag ctctggctag gccaacccgt tgttgcatcc caccagataa ctgactagga
     3301 tattggtctt taaatgaaag caaactagaa ttttctaatg ctttttctgc tttttcttgt
     3361 cgttcttctt taggtacacc acggacttct aacccgtatt ctgtattttc caagatcgtt
     3421 cgatgtggaa acagaccgaa gttttggaaa accatattga ttttatgacg acggacttct
     3481 cgcaattctt ctttgcttaa cttcgataca tcttctccat caatatagat actgcccgaa
     3541 gttggctcaa tcagacgatt caatagacgg atcaatgtgg atttcccact cccggaaagc
     3601 cccatgatca cgaagatttc tccttctttt acgtcaaaat tcacgtcata cacaccaacc
     3661 gttgcaccgg ttttctttaa aatctccgtt ttcggctggt gatcattcat catatctaaa
     3721 gcggcttgtg tctttttacc aaatatcttt gttaaatgtt ttacttctac ttttaccaaa
     3781 caaattctcc ttttttatgt aaattcattt ttctttctga aaaaagtgac cacactatgt
     3841 taacatcatg tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa
     3901 acttggctaa atctttgtaa ataaaaggtt tagccaagtt aatatttttt taatatttca
     3961 cacaatcatt ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta
     4021 tattcatttc caataaaatg aacgatgtcc cctgccgtaa atttagcata ggggccaggt
     4081 gaaacaagca gttcttcatt atgagaaatt gcgataactg tggcgccagt cgcctgccag
     4141 atattcaact cgctaattga acggtccaga tgcattgctt tttctgataa agataattca
     4201 aacggaagta aaggattcat cttatgtatt cgcttcgttt gattgaccag ctgatccaat
     4261 aattgagaaa aatgattcag ctcatcttgt tgcttctcca cacttgccat gatttctgct
     4321 tttgtatctt ggatggattg tacatcttga tattgcaata ggaaatcttt tgctttttcc
     4381 ttcgaagcaa caaatgcccc acttccatgc cgtacttcca tgattccaag atctaccagc
     4441 acattgatag ccttgcgggt agtctcagga gaagcattgt acgtactggc aagtgttgag
     4501 cgggcatgga tcttctcacc cactttatat cggttttcaa cgatacgttc ggctaaatca
     4561 acagcaatct gctggtagcg cgggcggacg atttttttct ttccagacat tttctttcct
     4621 ctttcttttt ttactttccc tcatcttact atgctctagc aaaataagca aaaaagggga
     4681 gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac tgggcaagct
     4741 gtttgacagt catcacaatt cttaccggat tttaaacgag tatagacaat tgcgcctgca
     4801 cttccaaaga ttaacacact taataagaat gtcgccatcc tattcctctc cttctaatga
     4861 caatatagta taaaactggt tcttggattt tggctgacgg aacaaaacat aaacgagtcc
     4921 tatcatcagt aaaatagcaa gatacgtccc taacgctacc tgtccaccct ccaataagtg
     4981 tcccaactga taaatcacaa aactaactaa ataagctaaa ccgcattgat agcctatggc
     5041 gatccaagtc catttcttgg cattcatttc ccgatggata gccccaattg ccgcaaaaca
     5101 aggcgcacaa agtaaattga aggctaacaa tgaataagct gctgcgggag tatatgtgtg
     5161 ttgtagcact tgccagattt ctctgccatt ttcggatact tccgcatgac caaacaaaat
     5221 accaaatgtg ccaataatat tttctttagc gaccaatcct gtaatcgctg cgactgttcc
     5281 ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc
     5341 taagatactt tggtcttcag aaacaggttg agcataaaaa ttataagaag agctgaacca
     5401 gatcacgata ctagaaacaa agataatcgt tcctgctttt ttaacaaaag acttgctgcg
     5461 gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat
     5521 gataaaagga gctgtatctc cagcaaacaa acgtgttttt ttcaagctga ttccagagaa
     5581 aataattgca gtcataccaa gaaaataggc agaaggagct acccaagatt gatgaggaaa
     5641 aaaggcccct gctactaagg caataatcgg caactttgca gaacaaggca tgaaggttgt
     5701 gaccatgatc gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc
     5761 tggcactcca catcctgtag caatcagcat aggaatgaag gattttcctg aaagaccgaa
     5821 tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc atatatccgc agtcttccag
     5881 aaagctcaag cataaaaaca atataatcaa ttgcggtaga aatcctaaca ctgctcccac
     5941 gcctgcgatg atgccgttca agatcagctc ctgcatccat cccgctactt gccattcagc
     6001 taacaatctg ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc
     6061 tgtccccatt gttcctactg tttgaatagc cagataatag atcagccaca tcacaaatgc
     6121 gaagatcggc aaggctaacc aacgattcgt tgcaatctga tcgattttgt cactcatcga
     6181 taattggaaa gaatcatttg acacaacaca gagtgtacat agttgggtaa taaattcata
     6241 acgttcattg accagaatcg tctcactatc gtcgtccaat agtttctctg ttaactggat
     6301 cagttgctcg atctcttttt gctgagaaac tgctatctcg taatcctctc ttaccttctg
     6361 atctcgttca aaatatttca ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac
     6421 atcttcaatt tcatgtaaag ctgcttctaa acgtggatca tatgtacagg gaacaggaat
     6481 agaaggccgc ttttttgcag actcctgaat cttttcgatt agtttttcga ttccttgctt
     6541 tttcaatacg ctgattggta aaactggtac accaagacca taggaaagct tttctagatt
     6601 gatttttttt ccttcttttt tgatgacatc catcatattc agaccaatga tcataggaat
     6661 gtctaaatcc aataactgcg tagttaaata aagatttcgt tctaaattgg tagcatcaat
     6721 aatgttgatg atcagttcag gctgttcctg aaccaaataa tctcttgcaa tcacttcttc
     6781 tggtgtataa ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt
     6841 ttttttcaac gatccttctt ttttttctac tgttacgccc ggccaattcc ctacatattg
     6901 attcgaacca gtcaagagat tgaacaagct agttttcccg ctgtttggat ttccgactaa
     6961 cgcgatattc attctttttc ctccctttgt atcaagatta gttcggcctc tgactttcgt
     7021 aacgtcagtt cataccctcg cactgtcagt tcgattggat ctcccagagg ggctagtttt
     7081 cggataacta tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg
     7141 ccttgtccgt ggattgctac gacgatccca gtctctcctg cttttaattg atcaagagaa
     7201 gtccagcttt cagaggaagc agtcttttta ttgacaaaga tttgacctag tatttcccga
     7261 cttactgcca ctcgatgact ttttaccagt aaaatggcat tgttgcctga taaattgacg
     7321 acgacgattt tacttccggc aacgatcccg atattttgca aatggcgttt tgttttttct
     7381 tctgtttcga ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc taaggtctgc
     7441 atattgattc ctctcttgtg aaataaatca tgtattaaaa tcattcatta tatgttctaa
     7501 ttttcttacg tgttacgcaa ataaatgaga atcattctca catagaatac tctcggtaat
     7561 tgagaatgtc aattatactt gtgatattat gagcgaatct ttagtattga tagataaaaa
     7621 tgagaaaaaa atatttcttc atagttttcc acaatttttt ctctattaaa tttgctattt
     7681 tcccacacga tacttgtttt cagctctttt ttcaaaaaca caaaacttag tattccacag
     7741 tttccacaac acaatatgta gtgtttgttg tttgactttt ttactaagag ttttatacta
     7801 tctattatag ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt
     7861 tctaaaaata attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt
     7921 gcttttgaag aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa
     7981 ggattccaaa gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct
     8041 gatcagttgc gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga
     8101 tttgaagggc ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc
     8161 ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg
     8221 tacgtatgag tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca
     8281 atcggccagt caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt
     8341 ttgaggaaaa tgtcaaacca aatacgatga cttttctatc tattatggaa aaaatcaatt
     8401 acttgatcga tcaagaatat atagaagcag aattcattca attgtattca ccatcattta
     8461 ttgaaaagct atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg
     8521 cttacaaatt ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa
     8581 cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag
     8641 cacttgctct tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct
     8701 tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg
     8761 acgatatgaa ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg
     8821 gcggcgtagg gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg
     8881 agggtgctgc aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt
     8941 ctaatcaatt aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag
     9001 acattattgc tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa
     9061 ctctttctct tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag
     9121 atatgtattt atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg
     9181 taaatattac agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa
     9241 tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt
     9301 acgtaatcaa tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga
     9361 gtaatttatg ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg
     9421 aatatgaagt attaggaacg gatatctcat gtaacttagg ctcgacaaat atcgtgaatt
     9481 tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta
     9541 ttacagatgc atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc
     9601 atacgattgg attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat
     9661 atggctcaga ggattcatta gactttacaa atatttactt catgctgttg aattattgga
     9721 cacttgttga aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa
     9781 aatcagctta tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa
     9841 aatcagaaaa agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga
     9901 atgctttgaa acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag
     9961 caccaaatgg ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc
    10021 gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc
    10081 tatccaatga gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa
    10141 tcgatgttta tgcaatggca caacaacacg tcgatcaagg catgagtttg actttattca
    10201 tgcgttctga aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac
    10261 gtgatttgaa tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg
    10321 tacgtacctt tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga
    10381 tctaatatca tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca
    10441 gaaacatatt atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact
    10501 tgggagaagc tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta
    10561 gatgattggc gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt
    10621 ttgacactgc tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc
    10681 cgcactcccc atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc
    10741 aaaagttatt cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt
    10801 gactggacaa atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac
    10861 aaacacggta caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc
    10921 tattcaggtt tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca
    10981 gagatcatca aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa
    11041 ttccaattag gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg
    11101 tacaatcttc tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat
    11161 ccaatcggct ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg
    11221 atgaacctag ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta
    11281 atgaacggga tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc
    11341 tatttgttag gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa
    11401 ggatttattt taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt
    11461 taaatgtaca ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac
    11521 tgtctaactt ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta
    11581 atattatcgt cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg
    11641 cgacacttcc tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt
    11701 tcccaaataa caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca
    11761 acgccatata caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg
    11821 ctccaataaa agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca
    11881 acaagctata aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca
    11941 aagcatctaa gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg
    12001 tttggcgtaa ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt
    12061 tgtgcccatc cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa
    12121 attgcatcat aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat
    12181 tgaacaaatt actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt
    12241 taaactcttc ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg
    12301 gcaagctctg ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag
    12361 ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga
    12421 tccgatgctt ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa
    12481 caagcaatgg taaaaaaact gccgaaagca ttttcaatat tggactaaaa atattcggca
    12541 tttgtaaaat aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata
    12601 attttgattg tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat
    12661 tctatcattt aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa
    12721 aataaacata ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga
    12781 caaaaattat actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg
    12841 taataagccc agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac
    12901 ttgatgtagg acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg
    12961 caactttgtg atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc
    13021 ttttggaaga tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac
    13081 gtcaaaattc acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc
    13141 tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat
    13201 cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga
    13261 atagtaaccc atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt
    13321 cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac
    13381 accactcacc acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg
    13441 tggatactct aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat
    13501 gatacatgct cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata
    13561 ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat
    13621 ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt
    13681 ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca
    13741 aaatttttgt catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat
    13801 tactcaacaa acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg
    13861 tcaaaacgct gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg
    13921 cttcttcatc gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat
    13981 gattcgttac aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg
    14041 ccatcaagac gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg
    14101 ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta
    14161 agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc
    14221 cattttgccc gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat
    14281 actttttgca tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga
    14341 atggtttttt atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac
    14401 cgcctagtcc aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc
    14461 ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt
    14521 ttgcaccttt gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag
    14581 cgttttttac acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat
    14641 tatagcattc tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat
    14701 tttcttgaca ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc
    14761 catgttgcat gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt
    14821 tatgaaaaag gagttattat gagtatactg acattaaaca acattaccca gcaatttggc
    14881 gacaaaatcc tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg
    14941 attggacaaa acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta
    15001 ccagatgacg gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat
    15061 gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa
    15121 tacgaaaagg aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat
    15181 tccttgctgg aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa
    15241 atcgacacct tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat
    15301 tcactgttga aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt
    15361 ttagaaaacc cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat
    15421 atccaatggc tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat
    15481 gaccaagaat ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt
    15541 actaagtata ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc
    15601 tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga
    15661 aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa
    15721 atcgaacggc tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg
    15781 cctattgtca cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg
    15841 ctacttgcac ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc
    15901 aacgggattg gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt
    15961 ggcgatttcc acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg
    16021 gaaaatccat tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc
    16081 aaagaattac gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct
    16141 ctcactacat taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac
    16201 caaggtaatt tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa
    16261 agcttacaag acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa
    16321 gaattttatc aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat
    16381 tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata
    16441 aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc
    16501 tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg
    16561 tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg
    16621 cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca
    16681 ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaatacag
    16741 tattatttcg gttgcttgct gcttcaccta gcccctgtac tcgtgtttga ttggcgactt
    16801 gaccaaattg attcccgacg tctaataaaa taatagctaa aagcaaaatg gccaaatggc
    16861 ttccgccaac caataaagca ataaagctta ttgtttgcat aacaagtccc atatacacga
    16921 tttttctttc agaataagaa tctgccagtc gtcccacgat aggtgcagca actgctccag
    16981 acaaaccaaa aatcgctaat atccctgctt cgaatgttcc ccaattgtag gcgggactgc
    17041 tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac
    17101 tgactgccgc ttctcttaat agacgttgag aagcaactaa atgcggcaaa cttttaagtg
    17161 aatcaaggta tgaaacagct gtactacttt gctggacaga acgcggcatt ttccagtaaa
    17221 gcagaagtgt aagaattccg actgcacata aagcaaataa atagatcatc cgccaagaaa
    17281 aaagactggc aacaatacca gataccgtcc gagaaagcaa aatccccacg agtaaaccgc
    17341 taagcaatgt acccatgacc ttccctcttg cttttggtcc cgctagtacc gcaccgtaag
    17401 gaatgatgat ttgcggcacg atcgaaagta atcctattaa aaaagaagcg catgcaaata
    17461 acgtaaacga aggcgcaaaa aaagcggcca acagagagac ggcagataac gctgccattc
    17521 gaatgatcaa ttttggacga tccaccacgt cccctaaagg aactaaaaac agtaacccta
    17581 acgcatagcc taattgggtc aacatcgtca atatcccgac tgcacttgtg ctaacagata
    17641 aactagctgc tacttttgtt ccaatcggct ggatgtaata catattggca acaacaacgc
    17701 cacaagtgat tgataatagt aaaataagcg aagttgttaa aggttttttt tcattactct
    17761 tcattgattt cccccatttc ctatcttagt ttacaactta tggaaaagat tggaaataga
    17821 aaaatacaac tattcgccac acttattatt tgtgcttttg ttttccttgt tttttcttgc
    17881 gatctttata aatgaagaca actaacccta ctagcacagt aacacttgca accaacgtga
    17941 ctgcagtttt gttatatccg tgaaacagag cttctccacc acacgcatac aacacagacg
    18001 aagggatgac tccaatcacg atcgacaata tgatttgctg tagaggcaat ttcatcgcat
    18061 ctgcagcaaa attgaccata gaagatggaa taaccggtat catatagcct aacattacac
    18121 ctatttttgg atgtttcatt tgtcggattg cttgcaccca atgattcgtt tcggttttct
    18181 tatctaaaaa ttttaagcga tgcataagga aaattgcagt gatgttacca agagcattac
    18241 ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct caaaccagca atgactccaa
    18301 ctactgaagt aggaatacct ggaacagcac acatcaccgc tgtcaatacg atcaagagta
    18361 tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg
    18421 gatttaaaaa taattggatg tctgtctgat attctaaata caaccgatag cccactacca
    18481 gtatcagaat gattcccaac gagatcaata tcaatcggat tattttcttt ttcatatttc
    18541 ctcctgaaaa aaatctatat tcattaaata atagtttaac ataaaaacaa aaaagcaccg
    18601 aaatccaaca taggatctca gtgcttttaa atagtaaaaa ttatttaaca gttacagatg
    18661 cgccaacttc ttcaagttta gctttcaatt cttcagcttc ttctttagat acgccttctt
    18721 taactggtgc aggagcgcca tcaactacag ctttagcttc tttcaagcct aaaccagttg
    18781 cttcacgaac tgctttgata actttaactt tttggtctcc agctgcagtt aattctacag
    18841 tgaattctgt ttgttcttca gcagcagcag cgccaccagc agcagctaca gctacaggag
    18901 cagcagcaga tacgccaaat tcttcttcaa tagctttaac taggtcgtta agttctagga
    18961 tagttgcttc ttttaactca gcaacgatgt tttcaatgtt caatgccatt ataatttcct
    19021 ccgtttaatt gttctttttt atttaatgct ttatgcggca attaagctgc atctccatct
    19081 tctttgtctg ccactgcttt gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca
    19141 gatagcaaca tagatagaag tccttcgcga tttggcaatt ttgccaatgc tgtgatttct
    19201 tccaatgaag agactttacc ttcgatgata ccgcctttga tttctaatgc ttttgcttct
    19261 ttagcaaatt cgtccatgat tttcgctggt gcgactacat cttcgttact gaatgcaaca
    19321 gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc cagctttttt agctgcacgt
    19381 gaaagaatag aatttttgat aactttcatt tctacgttag catcacgaag ttgtttacgt
    19441 aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa cgactactac tgatgcagct
    19501 gctgtaaatt tttcgcttac ttcatcaacg attgctgctt tttttgcgat aattgcttca
    19561 ctcatttttg agtttcacct cctgaatttt gatggaagag ttttttgcac tgaaaaggca
    19621 agtcactttt atagttactt tgctcaacca gtattattca tcacctattc acttcgttca
    19681 tagtgattct actcgctttg ctcgaaagga aactcctttc gctttgctca accagtattg
    19741 ttcatcatct cttcacttcg ttcatagtga ttcacaataa aaaactctat gccaccgcag
    19801 acatagaggg actattgaat atattcaata aacgtcctcg gtaggaaatt aaggcttgat
    19861 gccacctact gtcttcggta caagttaatt attaacctca actagcttag catgctaaac
    19921 tagttgagtc aagaattatt tttcagttgg atcaatcaac attctaatat ttaattagaa
    19981 agaagcttgg tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta catttttcat
    20041 gtattgacct ttagctgctg atggtttagc ttttagcaat acatcatgga ttgctgcaaa
    20101 gttttcaact aatttttcat cgtcaaatga aacttttccg attggtacat ggatatttcc
    20161 agctttgtcc acacggtaag ttactttacc agctttcact tcgtttactg cttttgttac
    20221 gtccattgta actgtaccag ttttagggtt tggcattaag ccttttggtc ctaatactcg
    20281 tcctagacga cctacagtag ccatcatatc aggtgttgca acaactacat caaagtcaaa
    20341 ccatccgttt tggatttttt gaaccatgtc gtcgtcacct acgaaatctg caccagctgc
    20401 ttcagcttct tttgctttgt ctcctttagc aaaaactaaa acagtttgtg ttttaccagt
    20461 tccgtttggt aatacaacag caccacggat ttgttggtct gcttttttag ggtctacgtt
    20521 caaacggtat gcaacttcaa ctgttgcatc aaattttgca atgtttgttt cttttgctaa
    20581 agctactgct tcggcaacag gataaacttt acttgaatca acttttttta atgcatcttg
    20641 catttttttg ctctttttag ccattttggt tcctccttga ttgtggttat aacggttgta
    20701 cctcccacgt gttctatatc tttcaatata gagccaactg agaagctgct gtcttagggt
    20761 gtatagatta ttctacagtg atccccatgc ttcgtgcagt tccttcaacc atgcgcattg
    20821 ctgcttcaac gttagctgcg tttaggtctt ccatttttaa ttcagcgatt tctcttactt
    20881 gatcgctaga aactttcgca actttatttt tgtttggttc gcctgaacct ttttcgattt
    20941 tagctgcttt tttcaataga acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac
    21001 ggtcttcgta tacagaaata acaaccggaa tgatcaaacc tgcttgatca gctgtacgag
    21061 cgttgaattc ttttgtgaat cccatgatat tgatacccgc ttgacctagt gcaggaccta
    21121 ctggtggagc tggagttgct ttacctgcag gaatttgcaa tttaacgatt ttttctactt
    21181 tctttgccac gagacatacc tccttaagtc cgtgatgtgg ttaattggag atgcatattt
    21241 ctcctcccac gttttatctg atctgaaaaa atccagacaa ataatgtgca aaacaatgca
    21301 cactggtaca gtatagcaaa tttatttgtt ttttcaagat ttttttctgc tatttttaca
    21361 ttttgcttga ataaacgcta aatttattct aaaaaaacaa caatttttcg ttctcatttc
    21421 ttcttttatg ttacacttgc gctagcatgg ttcaacagat gaaagcaaac aataacacgg
    21481 ttttttagac ccttgtacaa agattcctac ataacaaaat gaggtggaga aaatgaaaga
    21541 tttaaggtat agaagtgtct ttgatattat tggacctgtg atgattggtc caagtagctc
    21601 tcacactgcc ggagctgcta gaatcgggaa aatcatacgt agtatatttg gcgaacagcc
    21661 tgattctgta gatatttatc tatatgaatc atttgcaaaa acctatcggg ggcacggaac
    21721 agatattgca ttagttggcg gcctgttagg gatggaaccc gatgatgaac gtttagctga
    21781 ttctttgaaa ttggcatatg aagccggtat ggaagtcttg tttgttccta agagtgaaaa
    21841 agccgaacat ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa agctttcagt
    21901 cactggcatt tcgatcggcg gtggcaatat ccagatttct gaattgaatg gattcaaaat
    21961 ttctttaagc atgggtactc ctaccttgat cgttgtccac caagatgttc caggtatgat
    22021 tgctaaagtg actagtatac tttctgagac caatacgaat atcggcacta tgacagtgac
    22081 acgggaatca aaaggtgaaa atgccattat gataattgaa gtagatgatc ctcaagtaga
    22141 agaaacggta aaaagactca aacagctgcc aaatatcgac agcgtaaatt atttcgatta
    22201 aggagctaaa aaaatgtttt tatcgattga agaattagta gatcaagcaa aagcttatcc
    22261 aagtgtagca gaattgatga ttgcagttga aatggaaatg agtggccgtt cccgtagtca
    22321 catcatcgag atcatggaaa aaaacctctc agtaatggaa caatcaatca acgagggaaa
    22381 agaaggtgtt acctccgtca caggaattac cggtggtgat gcaaaaaaat tagatgatta
    22441 tttaaatgag gcagactttt taagtggaga aacaatctta aaagctgtac gcaatgctgt
    22501 agctgttaat gaagtcaatg caaaaatggg gttgatttgt gctacaccga ctgccggaag
    22561 cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat cggcttgatt tgacacatga
    22621 acagcaattg gaattcttat ttaccgctgg cgctttcggg ttagtcatcg caaacaacgc
    22681 ttctatcagt ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa gtgcaatggc
    22741 agcagcagct ttggtttgtg caaaaaatgg gactcctcat caagcagcac aagcagtagc
    22801 cattacattg aaaaatatga tgggactgat ttgcgatccc gttgcaggct tagttgaagt
    22861 cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct tttatctctg ctgatatggc
    22921 attagcgggt atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa tgtaccaagt
    22981 cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa ggtggcttag ctgttacgcc
    23041 aactgctaaa cgacttgcgg aagagatttt aaataagagg gcgtgataaa gctccttaat
    23101 ttcgagtatt aagagattag ttatcttcat aacaaaaaac gaagagagga ctaattgccc
    23161 ttctcttcgt tttttattaa tccatcaatt tcgtatattt tacttcatcg atcgtttttg
    23221 ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa acagatgtta
    23281 ctccttctgc cccacctaga ttcaacccat aaatcactgg tcggccaatc gcaactaagt
    23341 cagcaccact tgccagagct ttgaagacat gctctcctcg acggacacca gagtcgaaaa
    23401 tcactggtac acgtttgttg actacttcgg caatttttgg caatacttca aaggacgcag
    23461 gacctccatc tagttgacgt ccaccatgat tggataccca gataccatct gcccctgcag
    23521 agatagcgat tactgcatct tctggcgatt gtattccttt tacgataact ggaagatggg
    23581 tgatttcttt gattgttttg atgtcatctg gtgtcaagcc ttgttttgca gctgcataaa
    23641 tttctgcgat tccttttcct tcgccattac cacttgcact ttgttcgcta tacgcagcta
    23701 agttaggcat tggtaatgga aattggaatt gattgatgac gtcttcttca cggtaaccac
    23761 cgagcgttga atctgccgtt aaaatgatag ctttagcccc tgcttctact gctttgttca
    23821 aaatgaactc attgaatctg tcgtctttac tcatatataa ttggaaaaac tgcggtgcgc
    23881 ctggcgctgc atcggctgcg tctttgattg tcgtatttgc ataagtgctg atggaaaaga
    23941 ttgatccagc agcagctact cctttagctg tatcagcttc ccctttttca tgtgccaatc
    24001 cttgggccgc tgatggcgct tggataatcg gtgtatctaa ttcaattccg aaaacagatg
    24061 tatgcaaatc ggctgagtcg attccgcgta atatccgtgg catgattttc tttgtattaa
    24121 aagaagtcgt attttctttc atcgtccact catcttcaga accaccgcga atatagccaa
    24181 aagctcctgc ttccatgcgc tcttttacac gaccttctag ttaggcaatg tttacaatat
    24241 cgattggatg ttcttcatta cttgtatgat aagtcatagt agatccttct ttcatcttct
    24301 tgattacata taaatcatac taaacttaca aaaagtaagc aaacatttac tctcagatac
    24361 ttttatcgct attctttcca aataaatcga cttacaagct gatctacttc ttggttttca
    24421 tgaagcatac tatgagaggc attttcacct gtcactactt tttcctctat ttcatttccg
    24481 cgttgcttca acaacgcata gaccgctaat gcactgttta acggtactgt tccatcactc
    24541 aagtctgttt catctagttg accagcaatc aagaaaaatc gagtggttat aggaatatta
    24601 ccgatcattt gctggtaatc ttgatagcgg ttagaaacga cagcaggccc ctttgctaac
    24661 tcatctgtca gagactgcgc actatcatcg acaaaatcat taaaaggcgc gccaattgct
    24721 gttaatgtct gaacttgtgg caatgacgta tcctgtccat acgttcctaa atagcgtagt
    24781 gcactaactc ctcccatgga atgccccact atgttgactt tatctatgcc gtagttttct
    24841 ttcagataca aaagacaagt tttgatccat tctgcttgat tccactcgtt atttttatta
    24901 tcttcaaaca atacttggac catcggattg tctgcttttc cagacaaatc tcctgttgct
    24961 tgtatttgtc catctgttcc tactgttaaa accaattctt ttactgcttg ctgatttttt
    25021 tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc gtggaaaaaa
    25081 agtgttggaa tagtggtttt ttctatcact ttactggatt ctgccgtttt ttctttatca
    25141 gaaacttcag tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa caaactgata
    25201 aggactaaag ctgctgcagt tgcacgtttg cataaatttt tgattggttc tgtcatacac
    25261 ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc caatcatatg atcgacattt
    25321 caagtctctt tattaatcga ttttatctac ttgatcgaaa tcaagttctg tactggtttc
    25381 acgtccaaac atatcgatgt tgactttcaa tttttctttt tctccgtcaa cttctgttac
    25441 ttgtccttct aatccagcaa atgcgccttc gatgattttc acgacttcgc caacttctac
    25501 ttctagatca ttttgtcgca ggctcatacc gatagaacgc aagatatgat tgacttcttc
    25561 tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc ctgtcacacc
    25621 aggtgtgttg cggacaacat accatgaagc gtctgtcatg atcatttcaa caagtacgta
    25681 tcctgggaat gttttatgaa cgatttcttt ttctttccca tttttgactt cagtttcttt
    25741 ttcttcagga acaacgacac ggaaaataaa gtcggccatt ttcatacttt gcgcacgaga
    25801 ttcgatattt gcttttactt tgttttcata tcctgaatat gtatgcaaga cataccagtt
    25861 tttttcaaaa gattccattg aaggctcctc atttcattgt aaaataaaaa aacctcagta
    25921 cacccgaagt ttttctttcg cttcattata gcactaaaag tacgttgtgg ctagcgagtt
    25981 attttaaaat ccagccaaat aagctttgga tggctgtatc cattacgtaa aacatcaatc
    26041 cgaaaataat agaagtttcg atgactacta acgtatcttt acggagttgt tttctgcttg
    26101 gccaagttac ttgtttgatt tcatctttta cgctttttag aaatttcatg agacaacctc
    26161 ccttgctact gtttatttcg tttctttatg taatgtatat tgattacaat atttacaaaa
    26221 cttattgatt tccaaacgtt cgccgctttt tccttcactg actgattttg tgtagttgcg
    26281 agacccacaa acggaacaag cgagtgctgc ttttttcttt gccatttact tctgccccct
    26341 tgctgagaat cagacttata cttcataaca gtatcacgta actttttact tgtcaatttg
    26401 tgaaataaac tatctattgt tttggataaa attatgatat gattaatttg aaattaagct
    26461 aaaatgagac aattggaggt atagcgatgc tattaaaaac acttgtatat aagaaaaaag
    26521 atctgacaac cgttacagag actagtacat tagaagaagc attaaaaatt ttagaaactt
    26581 ccggataccg ttgtgtgccg atcttagatt cttcaggtaa tattttccgt ggaaacattt
    26641 acaaaatgca tatctatcgt cataaagcaa atggcggtga tatgacgctt cctgtcactc
    26701 atcttttgaa aaacgctaca aagttcattt acctggatac ttctttcttc aaagtattct
    26761 ttacgatcaa agagcttcct tatattgctg tccttgacag tgaaaatcat ttttatggta
    26821 ttttgacaca tagtacgttg cttaatatgc tctctcaatc ttggagcgta gaacaaggaa
    26881 gctatgtact gacaattgct tccacaggaa agcaaggaga tttggctgct attgcaaaaa
    26941 tcatttcaaa acacagcagc attgccagct gtattactct tgatatcgaa aaagacgaat
    27001 acattcgacg tacattgatc acgctaccgg ctaacactga agcgaaagta tgtgaaacga
    27061 tcgtcaacca cttagagaaa aagaattata aagtggtaga aattgaaaat ttgcaaaacc
    27121 aagaagcata atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt ttttcgcttt
    27181 ttataattag acacgctttt caaaatcaag cgcgcgatct aaataataca tcaatggtgc
    27241 tgaaacagcc atgacgataa actcacttaa tgcaagcgtg ccataggttg tccaaaatgc
    27301 ttggccacct gttggaacta acatataagc aatcagtccc atgctgatgg taaagaagat
    27361 cgtgttcaat actaaacgca tctttacatt tttcactttg ttttgcaata aagctgtcag
    27421 gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca tccatgatac caaaaccaaa
    27481 gaaggcatta tagacgatga ccccgcctaa aactccccaa agcaatttac ggttaaagac
    27541 aactaaatga ttcaaacttt ctgaaatacg aaactggatc ggccctgagg atacaggcgc
    27601 aactaaaata gttaacgcta gataaagagc catgatgatg ccatttacaa cgattacgga
    27661 taaccgttgt ctgcttttcg ttttttgcat tttctctcct cttttctcct agttttttta
    27721 acgcgggatg gttgatgaac cgcggttatg tgaaacaaac aataattata acagactttt
    27781 catttatttc aatccgtctt atctgctttt aaaaaagcaa aagcttaaac aatgtaaacg
    27841 tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa ggggtcataa gaattcccag
    27901 taaagcagac gaagtaggat ctgcaagaac agaattgttg tgaaacttaa ataaataaaa
    27961 aaggatgtgg gaatttgagc aacctcttac tagagaacgg tctagcaagc agctgaagat
    28021 ctaagtggac atgccgaacc ttggaacttc tccttacgta agtaatggat catgcgcttg
    28081 ctaggctttt ttgtgttttt cggtcttcgg acctttttca ttttcctaga cttctgctat
    28141 aataaccaag aattaatttt gtaacagatt tttttgaaaa aacaagtttc tttcattcaa
    28201 aaaatagaaa aagcttgcta attattggtt aaccagcaaa caggagtatt aaaatatctt
    28261 gttttattga atagcaggag gaaatcatga aattccatta tcatatattg atcaatccat
    28321 cggcaggcag cggaaatggc tataaagtag cagaaagaat tttaccggta ttaaaaaata
    28381 agcatattga ctatacactt cattatagtg aatacaaagg acacgatgct gagattgctg
    28441 aaactttggc aaaagaaaca ttgattcctt ggatagaaga agaacagaaa acaatcgata
    28501 ctttccccct tttgatcatc gtaggaggtg acggcacctt acatcaggta cttgatactt
    28561 tctatcagat ggaagtagaa ttcccagttg cttatattcc agcaggttct ggagatgatt
    28621 ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg attttagcag
    28681 ctcaatcacc agaaaaagtc cacataatgg cttatgaaga aaaaataagt gaaaaaaaag
    28741 gaatagctgt taataatttc ggtataggat tagatgcagc tatcgtccat gcgacaaatc
    28801 attcttccac aaaaaagcga ttgaacaaat acaacttagg atcattttcg tatttattct
    28861 ctattctccg tgctttattc actcaaaaag gatttccaat tctggtagat gcaggaggaa
    28921 aacaattaag tttttcaaac gcatttttat gtacagcaac gaagcatcct ttttttggcg
    28981 gaggaatcgc gattgttcca actgctgatg cgtctaaacc agtcattgat tttgtcgtgg
    29041 tcgaacgaat caatttcttt aaaatcttgt ggctcattct tttacttata cagaaaaaac
    29101 aattgaaatc gaaatatttc catcactaca caacaaacag attacgtatc gtttcaacaa
    29161 caccgcaaca tggtcaagaa gatggagaag aaatggaaaa acgacctttt gatatcacaa
    29221 taaccacaac taaacagttg ttttggtgtt aataaaagag gttttgtcag aagctacatc
    29281 tggaacaccg tttattcgga aaaaaggact gtgacacgac ttttgtcaca gtcctttttt
    29341 tactatgcag ttaattcttt gcccaattgg gttgccatcg atagtttctt tctttaccca
    29401 aaataaattg gatcattgaa aacaccgcag caaaagcaac taagacacaa ccattgaacc
    29461 aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag ttattatttt
    29521 gaacaaacca atatgttggt gtgaatgccg ctattttgct aaccatttct ggcaagtatt
    29581 ctgctggtac aaacacccca ccgataaagc aactgcctgt gataaaaata tctttgatcc
    29641 caccgctggt ttctgagttt tttaccaatg aagtgataca agccgaaaag ctaatgatcg
    29701 gcaataaaaa caagaaagta tttaaaatat gataccatac cgttatatcc cacgaggtgt
    29761 ggatcactaa cagaaccatt agaagtaaga agccactgaa caacagcagc gagtaactaa
    29821 tcaaactaaa ggttactttg cgattcaagt ttcgtttggt aatgggcgca ctctggtttc
    29881 gtttactgat ctctttgcga ttaaatgcta gattgatgac gctgaaaccg ctaaaaatgg
    29941 atagaaacaa cccataagac aagaaattaa aggttgtgcc cgtcaacagc tttttcatcc
    30001 gctgactata tcccgcactt agctcaactt ctccagtaac cgagagtgcc atttgtgtct
    30061 gttccatagc tgcatcgatt gttttatctt cttcttgaaa cagaatcagt gtttgcaaaa
    30121 aggtgttcac ttgctgtgta acaactgttt tagaaaaggc atctgggcga ctctgtatct
    30181 tgatcgtcgg catctttcct acttcgactt gttcggtaaa gtctgcaggc agatgtaaga
    30241 taatgtttac acgattaaaa taaagagcat catcgatttc tttttgtgag gttccatcca
    30301 atgtaacgac tgtttccttg tcattcaaat atttacgaag gccatcgact acaggcgact
    30361 cttcttcact aatcaaggcg attttcgcat cttgcagaga ggtattttca acatcaatat
    30421 tttgcgcaaa aaagaatgtg gcaacaacag tgataattac acctatcagt aaattcgatt
    30481 tatttgcttt aagcaatgtc caaatcgttt tataaagcgt cataacgcac ccctctttcc
    30541 atccaagcat ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag aagattggta
    30601 taaaaactag ccaaatcagt gtagaaataa agtttataca aactttcgct gatcaagttg
    30661 acgagattta attgaccaac aatcggaata tgaagatcaa tccaatactt cacttgttcc
    30721 gaccccatca taccagcaaa aaagctcatc gcaatcgtca ctgaaatcaa gatgctatcc
    30781 ttttgcacca gattcatttt tggtatcaaa ttaccaacca atgtacctaa caaaatcgca
    30841 cacaacgacc ccagagcggt aacaatcatc aaccattgcc aacgttcacc aaagtctact
    30901 tgatagataa aacgaaagac agctaacata atataaatct ctgtcaaaac aatcgtaaag
    30961 cttgctgcca aattagcgat tgaaactaac gattgctttc taggaatcaa ggacaagcga
    31021 ataccatttg acgattgatt tgcttgctga tcctgtacat tgcggacccc ccacatcgtc
    31081 ccgtagagaa tcgccattcc aatcagagtg aagaaataaa aacttttgaa actcatatta
    31141 cgacctgcat gaacttcttg aaaagatact tcttgttcaa aaacagtcat gatgggcgcg
    31201 agatcaacgt tctccacttc tgccacttta ctcgaaatta taccaatacg ctgtaaaaat
    31261 tgatccatga cgtttttcat caacgtctga ctcattcctg cttgacccac atggagcgaa
    31321 acagatgaac catcaatgag ataaaaacca gccaaatcat ctgcatttag cttttcattc
    31381 gcttctactt ccgatagttc ctgtacttca aataatggtt tatcattgac ttcgacttgt
    31441 tctaagactg tggtaaaatt ttccgaaagc gctgtttgat ccgctttgat tacaattcca
    31501 gtattaatcg tttcgagatt gccaccttga tcaatctcgc caaaggccgc cataaacatc
    31561 aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca tggatttatt tcgcagcagt
    31621 atttttgtgc gatatttata aaaatgccaa aacattattt cccctcctct agtctctcaa
    31681 atccttgcca gtaatctcta aaaagacatc attcaaggac gcttctttgg ttgctatttt
    31741 tccgtaactg atcttttctt cttgtaagat tgtaagaatc ggcaacaata ctgtatttag
    31801 ttcagtagtt gttacttgga gctgaccttt tttataagcg attccatcag aagctattgt
    31861 tcgctgacaa cacgttttct gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc
    31921 gtaccatatc ttttagagat tctttggttc ctttagcaat cacctgtcct tgatccatga
    31981 tcacgatctg gtcgcaaaga atttctactt cttccatgta atgtgttgta tagacaatgg
    32041 ttgctccttg tcgattcagc tccttgatac tttctaaaat tttattccga ctttgggggt
    32101 ctacagctac tgtcggctca tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg
    32161 cgatgttcaa ccgacgtttc aagccaccac taagttctcc tggacgaaac gaaacatatt
    32221 tttctaatcc cactaattga atcacctctt caatcaaggc tttccttttc tctttttcgg
    32281 aaacatataa accgcaaaaa taatcgatat tttcttgcac acttagttcg tcaaagaccg
    32341 caacgtcttg aggaacgacc ccaatcattg ccttgacagc ataattttca ggagtcattg
    32401 ttgtcccgaa caatttaatg gttcctttgt cataatttaa caaagaaagc atacagttga
    32461 tggcagtcga tttcccactg ccgtttggtc ctaacagacc taagacttct ccttcttgta
    32521 cttgcagatc aaaatgattc aatgctacaa aatctccata gcgtttgact aatttatcaa
    32581 tttcgataat catcatgaaa aactccttcc aaatttcttg ttgatctgac tataataaag
    32641 tgcaagaaaa gaaactagtg acgattggct aaaccacaca tgacatttgt cacaatgaag
    32701 gagtctgcta tgcataaatc tcggctactc aacgaaacta gctttctact ggttatctca
    32761 ttgcttttgc tggtaagtca aggattcgtt gctatgcaag ttatctattt attgatcgcc
    32821 gttttattca ctggtgtcag ttatttactg cctacaaagc caaattggat tttccaagtt
    32881 attctatttc ttttatgtgc ctttttacct gatcttgtcc tgtttctgcc agcgacgatg
    32941 cgagtgatcg cccctgaaga aagccacctt gattacttgt ttttgttagg ttttttgttg
    33001 atttcgacgg ttggtaatca tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt
    33061 ttttcctttt acttacgaaa aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt
    33121 gtctccaaag atactcattg ggaaaaacaa caagagttat cggaacaaaa taaagcattg
    33181 gccctgtcac aagaaacaca attagccttg caaattgtgg aagaacggaa ccgaattgcc
    33241 cgagacatcc atgacaatgt cggtcatctg ctgtcaagtg cgattttaca aattggtgcg
    33301 cttgaaatga tcaatcaaga accaaaacta aaagaaccat tagaaaattt aagtgccacc
    33361 gttcacactg ggatggatcg gattcgtcaa agtgtccatg acctccatga gacatcgatc
    33421 tcttttcaac actccttaga gtttctgatc gctgattttc cttcttctgt tacgattgaa
    33481 ggaaacttgt ttgaaggatt gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa
    33541 gaagccttaa ccaacagtat caaacatagt catgccaaaa ccgttgtttt acattttcga
    33601 acgttgccgg ccttttatcg cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat
    33661 tctgctcccg gtattggttt gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa
    33721 gtccatattt tccaagaaaa ccagcaattt cagttaacga tcattttacc aagggaggga
    33781 caccaatgat aacagtagtc attattgaga tgatccattt gtgacgaaat cgttacaaac
    33841 cattttagaa agcacgaaag agatccgtgt gttaggcatt ggtcattgtg ccaaggatgc
    33901 gctagtctta tacgaaaccc atcgtcccga tgttttgctg acggacatcc gcatgcctaa
    33961 acaaacgggc atcgatgctg ccaaagaaat tttggctaaa tttcccaaag ccatcctttt
    34021 gcttttgacg acattcaaag atgaagaata catccgcgaa gcattttcca tcggtgtcaa
    34081 agggtattta atcaaacaga acttgcaagc gattattcct tctgtcaaat ccgcttataa
    34141 tggacaggcc gtatttggta atgaaatcat cgagactttc actcaaatga tgaagaataa
    34201 accgacgatt gatagtcatg cttatgcttc ttttagtgaa cgtgagcttg caatcatcaa
    34261 agaagtagct gccgggaaaa acaataaaga aattgcagat gccttgtact tgagtgacgg
    34321 cactgttcgc aattacatca gccaattact agaaaagcta gatttacggg atcgcacgca
    34381 attggctatc tatttttatc agcatttgca ataagtctta tctaacacaa aaaaacctga
    34441 atgatgctcc ttcgcatcat tcaggttttt ttcacatctt ctattcaatt gtcccgcaaa
    34501 ccatttcctc acgattgaaa gaatcaagga ttcgcagggc tgccaggatt cgttggattg
    34561 ttcggcgtgt taggcggaaa aataggcgcc ttgtctaacg ttttttctag ttttgattct
    34621 tcacgatcgt ctcgttctac atcaggactt tcattcaatt tgaattcttt ttccatctca
    34681 tctaactttt taaagtcatt gccagattct tttgctaatt tcgctaattt atcttcggtt
    34741 tctttagcca taaagtaacg cccccctttt gatttcagct tagcatatca cactgaaaaa
    34801 acaaaagaaa acgctaacca acgatcattt ttagctattt ctcatcttta aaatcaagca
    34861 ttttttgccg gtataaaatc gtagggagaa ctgttttcca taccaatcat cggatctact
    34921 aactggttat cgatcacttc tgctgtaaga acgaaatctt tagaaacagc aggttctacc
    34981 tcttctttct tttcttctcc aacaaatgaa gaaaacagat ctggttccac tgtttccatt
    35041 acttggtttt tcgtttcatt cgtcccactg tcatcttcta caggtagctt ttcttttaca
    35101 acccgtggct cttttgtata gttcccatct tcaaatggat catttccgac caaagaatct
    35161 tcataagctg ctagtttggt ccggagagtg atattcattt tatcattatc catgatcctt
    35221 gatttcaacg tagtcatccg gcttgctgat tcattcgcta cacaagtgac ataggcttgc
    35281 tcttccccta acaaaatcaa cagttcctta ctgcgagtca ctgccgtgta gagcaaatta
    35341 cgctgaagca tccgctgata ttgatggacc atgggcaaaa taaccatctt gaattcactg
    35401 ccttgtgatt tatggattga gcaacaataa gaaagagtga ttttattcca ttcgtttctt
    35461 ttataagtga cctcatttgc atcaaactgg ataactaatt catccacttt atcttcagaa
    35521 tcttttgcca agatgatacc cgtgatttct cccatatctc cattgaatac attcaattcg
    35581 ggagtattga ctaattggag gactttgtct cctatccgat aaacggtgtc gttccaatgt
    35641 acttcctttt ttccaggttt ccctggattg aatatctcct gcatcatttt atttaacgca
    35701 tcgatccctg caggtccccg atacattggt gctaatactt ggatatcttg tgcacggaat
    35761 cccttatttt tcgctttttc cacaatttta gagatcaagg gttcaatttg ataagcatca
    35821 cttcggaaat aggaacgatc tttttgattc tttgtgaaat cttttggcag acgtccttct
    35881 ttgatctcat gggcaagagg aataatacta gaaccatcac cttgccgata aatctgattg
    35941 agttccattt tgggaatttg ttcgatttcc aaaagatcat ggagtacttg accaggaccg
    36001 acagagggaa gctgatcttt gtctcctact aagataactt gcatattcgt cggaatcgct
    36061 ttgaataatg tgttagccag ccatgtatcg accatagaca tctcatcaac aatcaaaagt
    36121 ccgccttcta attctttcgt actgactgaa ggtgtttttt cccgtccatt cagccccaga
    36181 agacgatgga tcgtactggc aggcagacct gtcgtttcgt tcattcgttt agctgctcgg
    36241 ccagttggtg ctgcaagtag aataggaaaa atttcttggg tatattttga aggatccaat
    36301 tccagtccgt tcaactctgc aaaaagattg acgattccgt tgatcacagt agttttcccc
    36361 gtacctggtc ctcctgttaa aataaataga ggggaacgga ttgcttcagc gatagcttgt
    36421 tcttgcgaag gtccatactg tatgccgaat attttttcga tacgccgaat attcttctgc
    36481 acttcttttt caggataatg gatttctttt ttacgactga gaaggcgctg gatcgaatta
    36541 ccgatccccc attcagaaaa gtacaaactg ttttcgtaaa gattagtctc ttcttgttgt
    36601 atctttcctt cttcaacaag ttggataatt acagtagcaa cctgatcagg ctggatttca
    36661 attgggcgac ttgtttctaa aatacggatc gtttgctgaa gcaatgtctc cgccggtacg
    36721 tatgtatctc ctgtttgaac tgcatgctga aaaatctgat gcaagatagc tgctcgaatt
    36781 cttctcggtg aatctgctgc aataccgatt tgttcggcta gattatctgc acgtttgaac
    36841 ccgattcctt ctatgtcttc gactagctgg tacggatttt cttcgatgat atccaaagac
    36901 tcgttcttat aggcctggta gatactgaaa gctaactgac taccgaaccc gtaacgattc
    36961 agtcccatga tcacttgatc catcccgtgg tttagccgaa tcgtctcagc aagcatttct
    37021 ctttttggct tcgtcaaacc ggagatttgt tttaataccg aaggatcagc aatgattttt
    37081 tcgatagcat cttctcccag caattctaca attttttctg ctgttttctt cccgattcct
    37141 gggaattttt cgctggaaag atacgctacg atcccatttg cagttgttgg cttttcttgc
    37201 tcatatcttt cgagtttaag ctgccttccg tatttaggat gatccactaa ttctccataa
    37261 aaacgataag tctcattttc ttgaatatcg ccgaaacttc cggtgactac aatctctttt
    37321 tcacggtaat cagctgtggt ttcactgatg tgtaccaaaa gcactttgta aaaattacta
    37381 ggattttgaa aaaaaatcgc ggcaactgta ccaacgaaat aaggcatttc ttctggctgc
    37441 atcggaagcc gcctccttct tctatagtaa aggttctggt gctgtttcag ttcctaaaaa
    37501 atctgtttgg ttccatactt tcagttcata tggcaataat ctgcaaggct cttcagtctc
    37561 taaaatcgtc aaactactat tgaacaatcc acccatttca cgcaactcac ttagctcttt
    37621 tcctgtcatc cattgaatgg ctgcagttaa agaagctcca tgtcctacga ataaaagtgg
    37681 gccatcttta tgttgggcaa ccgcatctgc tacaacagtt tcgatacgtg tgattgccga
    37741 ttcaatcgtt tctccgtgaa aaggagaagg atcatacaga tctaaatgat tacgcaaatt
    37801 cggcagatta tccggatatt tctggtacat ttcatcgatc gactgacctt ctaaagtacc
    37861 cagccctagt tctcttagct tatctgtata aaaaatctct accggacgag caagctcttt
    37921 gttgatacct tctgctgtat cacgtgctcg ctttgctgta ctggaataaa ttgctttaaa
    37981 aggaacatat tttacctttt tcccaaatgc tttgatttct tcgtaacttt gtggcaaaag
    38041 cggagaatcg ccattgctcc cttgaaaacg tctttcctga ttccattgtg tcttcccatg
    38101 tcgggtaaaa tatagttcca tacgcagcaa actcctgtct ttcttttatt ttactctata
    38161 ataaactgtc taatccagct tgtcattttc atagtgatgc gtcaattcta aattagggaa
    38221 tccttgctgg cgaagtgctt catacactat cattgcagct gtattcgata aattcaatga
    38281 gcggacatgc tcatcattca tagggatgcg taaacacttt tcttcgtttt cacgcataaa
    38341 tgtttctggc aaaccggttg tttcttttcc aaataaaaag aagtgctctt ctccatcagc
    38401 aaaattttcg tcactatata ctttatgggc aaattttgag atcaaatgta gtttacgatt
    38461 acctaaaaat gttaaaaaag cttctaaatt actgtggtaa gtaatattta catcattcca
    38521 ataatccaac cctgcccgtt ttagatgttt gtcatcagtt gaaaaaccta gtggctcgat
    38581 taaatgaagc ggagtattcg tggctgcaca cgtacgggca atatttccag tgtttgcagg
    38641 gattaatggt tcaaataaaa cgatatgatt catcaggatc attcctctca tatttatgtt
    38701 cgtttctatt ttctgacaat ttgatttttg cataaaaaaa aacgtatcaa ctcggtgtca
    38761 gtcactgcga gtcgttacgt tagtgaagct cttctcacta acttttatca gaacaggaac
    38821 ctgataaaaa ccaatgaaaa aacaacttgt tgctaatata acaccctcaa cttttttttg
    38881 caacctctac tcattaaaaa ctgctagaaa aacgttttct cttagttcct tttaataatt
    38941 ctatgtcaat tgtaatcttc ttcaaaggat tcttatataa gtctgctttg attcgctctt
    39001 ctacctgcca ttctaaaggt gacatggcca aaagatcttt ttgattttcc tctggtatct
    39061 caaattcata ggttacacgc tgacgttcag ttaatttcat tgcttcagca aacttggcta
    39121 ctacacgttc attggaataa ctctgttttt caggttgatt cggataaaaa gcctgacgta
    39181 gttcctttaa ataattttct tgtggcacca ccttcaacac ttctccatct tctgagagta
    39241 cacgttggaa ttcttggtaa tgagaaggcg aaaagatatt taaaaccgcg ttgaatgttt
    39301 gatcagcaaa cggcaaattg gtcaaatcag ctacacacca aaatgcttcg gcagattgtt
    39361 ctgtagctgc gtagactcct tcttttgaaa tatcaaatcc aacagcattt tgaatctttt
    39421 tcaattttga aagttcattc aagaagctac cttctccgca gccaacatct aatagcgaga
    39481 cttcttcagg taaatgctgc gcaatagtct caataacggg ctgatacatg ccagattgga
    39541 tcattctacg ccttggaacg aacattcctt gatcgtattc tgttttaacc gcatgggata
    39601 aaaaataaag tgttcctttt ttggaaaggt caaaacggtg attatttttg catatcaagc
    39661 cattatcaga cttttttact tcttcttggc aaaccggaca gcggaacagc tgatgatttt
    39721 ctgccaaaaa ctggcgtcct cggtcaattt tttttaacat agttccccct catttctgtt
    39781 ttatcttacc gttagatgct cactttggca actgctaaaa atatgataca atgcctagaa
    39841 gaaagtgaaa gataaaggag aagattagat gatcaaagaa ttttgcgctg aaaactacac
    39901 atctattcct ctagcaattg ctagaggagc cgatcggatc gaattgtgtg acaacttagc
    39961 ggtcggtggc acaaccccga gtactggtgt gatcgaagaa acgttaagtt atgctgggga
    40021 aaaagatatc cccgtaatga cgattattcg tcctagaggg ggcgacttcg tctataacga
    40081 tatcgaattg aaaatcatgc atactgattt gattgaagca aagaaattag ggacggatgg
    40141 cgtagtattt ggttgtctta ctccttcagg ctggctagac gaagaggcct tagaactatt
    40201 gatcgaaaca gcagaagggt tgcagatcac cttccatatg gcttttgaca gtattcctga
    40261 agatcggcaa ttcgaagcaa tcgattggct ggtagaacat ggtgttgacc gtatcttgac
    40321 tcatggcggt cctgctgaca aaactattgt agaaaatttc ccacggctta aagaattgat
    40381 tgcctataca gatggccgct taatcatttt gcctggtggt ggtgtcactg cagagaatgc
    40441 agcacatgta gctaaagaat tgaacgtaag cgaagtacac ggaaccaaaa tcgtggatct
    40501 tcagttataa tcttataaac aaaaaaacat ttgcaacagg cataatgctt attgcaaatg
    40561 tttttgtttt tttacataaa atagcccata aataaagaag caatattgaa ataaatcagt
    40621 acactcgtca aatcgctcaa agtcgtgata aagggaccgc ttgctactgc tggatcaaat
    40681 cctagacgat ccattagcat cgggattaaa ctacctgcaa gattcgccac tgtgattgca
    40741 aatagcatag ccatccctat gacaaacccc aagataaaat tatgtttcca aaaacctacg
    40801 attagaaaaa tcgtgatccc cgtaacagct cctgtgacaa cgccggtcaa gacttcagcc
    40861 aaaacaagtc gaacaaatcc atgatctttc tcatcagaaa tcgcaagtcg acgaacagcc
    40921 acagctagcg actgggttcc agcattaccg gcagttcctg taatcagcga aatgaagact
    40981 gctaaaatac tcgcttcact taccaagtct tcatagtgac tgattaaaga agcggtcgac
    41041 atacctaaaa acaacagagt gatcagccaa ggcaaacgtc gagacgctgc ctttactgga
    41101 ttttcattaa tttcttccac attgaccccg gcaagtcctg aataatcgct agcagcttca
    41161 tcatcaataa cgtcgatgat atcatcgact gtgacgattc ctaacaaatg atcatcataa
    41221 tcggtaacag gaagagctaa gaagtcataa tctctaaatg tttgtgccac atcttcttgg
    41281 tcgtcgccta catgaactga aagcactcgt tcgctcatca gatcagaaat cattgcgtcg
    41341 tcatcattca cgattaggtc gcgcagagaa ataaccccta ctagctgacc ttcttgatta
    41401 acgacataga tgtaatagat tgtttcagct acatctgctt cgttcttcag cacgtacatc
    41461 gcagagcgaa cagtttgatt ggctacgatc gaaacaaatt cagttgtcat gatcgcgcca
    41521 gccgtttcat cctcgtaatg gagaagttcc ttgatttcac tggcatcgtc tgccgacatc
    41581 aaacttaaat atttagcaat ttgcttttta tccagcgtat taagcaagtc aacagcatta
    41641 tctgtataca tctcagaaag catatcagct gcatagctag gacgcatttc atctaggtat
    41701 tctttcatat gttcgttgtc ttcttcaatc acatcgaaca tatccgctaa ttctttaggc
    41761 gacagatagg aatagatcag ctgcctgttc tcttctgtaa gagattggta gaattgcccc
    41821 tgctcataga tatgcatctc taaaaaactg tttctgaatg cctgaatatt attttccctt
    41881 aaatcttgag tcaactgggc aaatctttcc tctaattctt gattttcatc caaaccaatc
    41941 tcacctcgcc ttattcttca aaccgtctca caactgcctc catgtctgcg gctaaaggct
    42001 gcttgaactc cagcaactct tctgtaaatg gatggtaaaa acgcagatca taacaatgaa
    42061 gggcttgtct ttttattccc agatccattc gcccgccata catatcatca ccgagcaggg
    42121 cacagccaat tgcggaaaaa tgaactctta tttgatgtgt ccttcctgta tgcagctgga
    42181 tatcaactaa cgcgatttct gttagtcgct tctctatcca gtactcagtc tgtgccgaac
    42241 gtccttcatc agagaccatt cgcttcaata gcgaagaata atctcgggcg atcggcaaat
    42301 cgatatgtcc atgtgccgat aaatctgaaa gccttccact gactaaggcc tgatattttt
    42361 tcacaaactt cttttcacga agttgcatat ctaattttgc gtgcgcaaat ccatgctttg
    42421 caaaaagcat caaaccagat gtatcccgat ctagtcgcgt caccacatgg attacttggt
    42481 tttcgtaacc ttgtttttta taataagcct ttacacgatt agccattgtc ccatttggat
    42541 ggtattgggc aggaatagac gaaacgcctg ctggtttatt tactaccaga acgtgttcat
    42601 cttcatacac gatatcgatc ggtgtttcat ccaaaagtac ggtttcatgt tctccttcag
    42661 caggaatcac aatggttacc ttatcatcct tagctagaga aaacaagaca ttttctactt
    42721 gatcattaac tttgatttga ccgccttgaa attttatttt cgctaacaga ccctttgaga
    42781 tccctttttc ttttaaaaaa tattttactt gctgagaatg atctttctca taatcccatg
    42841 taaactgcat atcaatcctc actaataaag gcatctttca cacgatgcca aaaatgcata
    42901 tgtcgataag aagcaaaatg gatccgttct tctgctattc gataataaat tgagcggata
    42961 tttgcttttg cgacattcaa ttgatcaacc gttacaagat aatcttctgt attttccaat
    43021 tttatttcta gccattcatc atgggcaatc acgatcggag aacccaaagt acgaaacact
    43081 cgattattca gtgaagcaat ctctgccaac tgaaaggcat tgatactagg atggatcaca
    43141 gcaccgccga cacttttatt atatgctgtc gaaccagtag gcgtagaaat cgtcaatcca
    43201 tctcctctaa aactttcaaa cagctcgttt ttgatgtata catcggctac catcgtacga
    43261 ttggcccgtt taatcgttga ttcattcagt gctaaaaaat gctggtccgg tgtttcatcc
    43321 aaataactga tccgaacatc cagcaaggga taactaacac tttgttcccg gttcgttcgt
    43381 aagctgtcta ctagttcttc cagctcgtaa tcccgccaat ccgtgtaaaa ccctaaatga
    43441 cctgtatgca cgcccaagaa acgcacatca ttcagacaat gactgtagtg atggaacgcc
    43501 gacaataatg tgccgtctcc gccgactgag atgaccaatt ctggctcatg atcatctcgt
    43561 tgaatccctg cttgttctaa aagtgcgttc aaacgttctg tcacttctat agtctttgct
    43621 tctttattat gaacgatcgc tactttcatc ccctgcctcc tctctttcgc tttctttctt
    43681 caaaagcgtt cgatagtaga catcatctga ttttcctctt ccatgggaga ataaccgctg
    43741 tgcttcttgg atttcttcac ggatactgga catctcttca tccaactgat aggcagcttc
    43801 tgccgcacgc tgaagtcgtt cactcatttc ttctggaaaa acgccttgat atttataatt
    43861 cagcgaatgc tcaatcgttg cccagaaatt catcgcaaga gtccgaatct ggatttccgc
    43921 caaaattttc ttctcaccgc tgatcagctg tacaggatac tcgatcacca gatgataaga
    43981 acggtagcca cttgcttttt tatttgtgat gtaatctcgt tcttggagaa ctttcatgtc
    44041 ttttctttta cggatgatct caacaacttg atggatatct tctacaaact gacacatgat
    44101 ccttaacccg gcaatatcag acatttcttc ttctaaacgt cccattggaa tatgccgtaa
    44161 ttgtgatttt gttacaatac tatcgactgg cttgactctc ccagtgacaa attcaatcgg
    44221 gacatgtcga ttttgttcac ggaactgttt gcggatcccc cgcagtttca ctttcaattc
    44281 tgatacagtt tgttcatatg gtgctaaaaa tgcttcccaa tctcgttcca tagctgatac
    44341 ctctacttca ttaacattca ttgtttattc taccatatat gcaaagtttt ttcgtactct
    44401 tgatttatgc attgaccaaa aaaagaaaat taggcaaaat agacaaaaag gagtcgatca
    44461 aatgacacaa atgcttgaaa tcgaatataa atctatgctg acaaagcaag agtacgagat
    44521 attcatcgct cactatcaat taacagataa agatttccgt gtacagacta atatttattt
    44581 cgataccgtt gatgaacagc tgaagaaaca aaattgtggc ctacggatcc gtttcgttgg
    44641 tcatcaagct gaatatacat taaaaacacc tgctgaaaaa gggcgtctcg aaacaacgga
    44701 tacctttata acagaagaag cagaagcatt tatcttcgaa aaacgtctgc ctcgttcagg
    44761 cgcagttttt caaaaacttt ctgatttgaa tatcgatcct gcttcattaa tccaaaccgg
    44821 aaagcttaca acaaaacggg ctgaattccc tattgaagaa ggacttttag ctattgatga
    44881 aagctgggga gacaatctac acgattttga actggaacta gaggtaaggg gatgcatctg
    44941 tgggaaaaat tgcttttata aattttttaa aacgtttttc tttaccttat cgtcctgcta
    45001 agaacaagat ccaacgtatg cttgaagcta aaaattagaa acctttcatt ttcacaaggc
    45061 acttgagttc atttttttac ttttttctgt taagttatag gatgtgaaag aatctagtca
    45121 ctagttgtat agaatctatt ttgggggaga ttcatatgat cgaaatctat ttatttgtta
    45181 atcctttagg ggaatactgt ttcgaaatgg aacaacagtt attacaattt atcgaagaag
    45241 agtacgggac accatcaaaa gaaaagatgc aatttcgctt tcttcctttg gtcaatttgc
    45301 agacaattgg ggatgtcatg caacaaaaag gaatttctca gaacaatctt gaggaacgta
    45361 atcacttatt ctcaatgact tattctgcag cattggactg caaagcggcg cagtttcaag
    45421 gaaagaaaaa aggccgccgt tttttgatca aattgcaaga agcagtcggc tgtaatgaca
    45481 taccatattc tcaagaactt gctgaatcca tatttgaaga aatcggcggc gatttgaata
    45541 tgtttaaaga agatcgtcag tctgacttcg ttaaagaagt attcttttct gatcaaaaaa
    45601 ttgctcggga aatgggcatc acaaaacacc cttctgcagt cgtttacaat tatgcctgcg
    45661 accgtgattt tggtgtttta gtagaagtaa aaggtacaag tagcctttgg agaatcacag
    45721 agctgtgtaa aacaacagat aactcatatc aagtttttaa tggtgaacat ttacatacaa
    45781 atgattctca gttccattca agaactagcc atttacactt gctttctaat taaaaaaata
    45841 cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga gaaaatcaaa
    45901 aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg ttgttcaaac
    45961 atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc ttttttcatt
    46021 acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt ctctagtgcc
    46081 tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa gccagtcgag
    46141 tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc gaatttaatt
    46201 tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc ataactatca
    46261 cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat gaaatgctcg
    46321 aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa gtaatggttg
    46381 agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc cgttaggata
    46441 ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc gtacacataa
    46501 ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg gacaagcgta
    46561 aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt gtcatagctt
    46621 cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat ccaacgttca
    46681 gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag tgcttctaaa
    46741 gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa gaccggtgta
    46801 taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa ttccatgtat
    46861 cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta tacactttca
    46921 ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg gactttcgct
    46981 ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt tctaaattgt
    47041 tcgtaaacac tgtacaatcc cttaaatgct gcttcgcgca cccttcgatc cgtgctttct
    47101 agcaactgac catacacgcc atgagataat tggactattt caccattttc tccttcaatc
    47161 gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga agcatcaaag
    47221 atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg ggctcgatta
    47281 tctactattt gctggatata atggcgatag acttccaatt ttggttcttc tttgaaatat
    47341 tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga aacggcttca
    47401 ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc tgtatttcca
    47461 gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg gcgataaacc
    47521 ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag tgttcctttg
    47581 tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc aaactcttgg
    47641 tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc ttctcgtttt
    47701 ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct atctatcaaa
    47761 atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc attcatgtgt
    47821 ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc gattcctagc
    47881 ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt taaaaaactg
    47941 ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat gttcttttat
    48001 ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt cttcgtacaa
    48061 cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt tttgatagat
    48121 ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt gtatggatac
    48181 tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat cttcactcac
    48241 tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata tttctttaag
    48301 gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg atacagtctc
    48361 tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc agcaatgcac
    48421 tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt gcgattgtcc
    48481 aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat aattttgggt
    48541 tctttcaata aatctctggt gagacagtct agaacattgg atttctattg ctagattcgc
    48601 caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa atactggttc
    48661 tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct cttttgattc
    48721 tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag cacatttttt
    48781 ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt tctttccttt
    48841 ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca gcatttttct
    48901 ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg cgtgaaaaag
    48961 ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata tttttcgttt
    49021 ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa aaaagactgc
    49081 gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat aaaatttcgt
    49141 tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg tttctggtgt
    49201 acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta catccgcttg
    49261 gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga ataaatacaa
    49321 atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt gttcaaaatc
    49381 ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag ataattcttc
    49441 ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt ctgaatcaac
    49501 agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga taaacagttc
    49561 taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc ggaactcctc
    49621 ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt tgtgattgcc
    49681 aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa ttagtacacg
    49741 gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt tgccacaatg
    49801 tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa atctcgctgt
    49861 taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt atttaactat
    49921 aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc cctctctatt
    49981 tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa tactttttgt
    50041 aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttt
//
LOCUS       DAZKCC010000012        49852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000012 DAZKCC010000000
VERSION     DAZKCC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.012"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8VH_000731"
     CDS             complement(105..629)
                     /locus_tag="ACG8VH_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP9109310.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8VH_000732"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8VH_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9109311.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8VH_000733"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8VH_000733"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9109312.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8VH_000734"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8VH_000734"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9109313.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8VH_000735"
     CDS             3157..4026
                     /locus_tag="ACG8VH_000735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9109314.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8VH_000736"
     CDS             4052..4567
                     /locus_tag="ACG8VH_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP9109315.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8VH_000737"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8VH_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109316.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8VH_000738"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8VH_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109317.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8VH_000739"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8VH_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109318.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8VH_000740"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8VH_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9109319.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8VH_000741"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8VH_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9109320.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="ACG8VH_000742"
     CDS             complement(7873..9090)
                     /locus_tag="ACG8VH_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9109321.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="ACG8VH_000743"
     CDS             complement(9348..10625)
                     /locus_tag="ACG8VH_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109322.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8VH_000744"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8VH_000744"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP9109323.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="ACG8VH_000745"
     CDS             complement(11547..12491)
                     /locus_tag="ACG8VH_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP9109324.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="ACG8VH_000746"
     CDS             complement(12493..13179)
                     /locus_tag="ACG8VH_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP9109325.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13653..13973
                     /locus_tag="ACG8VH_000747"
     CDS             13653..13973
                     /locus_tag="ACG8VH_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9109326.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14127..15032
                     /locus_tag="ACG8VH_000748"
     CDS             14127..15032
                     /locus_tag="ACG8VH_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109327.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            15029..15802
                     /locus_tag="ACG8VH_000749"
     CDS             15029..15802
                     /locus_tag="ACG8VH_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9109328.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15983..17407)
                     /locus_tag="ACG8VH_000750"
     CDS             complement(15983..17407)
                     /locus_tag="ACG8VH_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9109329.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17618..18064
                     /gene="nrdI"
                     /locus_tag="ACG8VH_000751"
     CDS             17618..18064
                     /gene="nrdI"
                     /locus_tag="ACG8VH_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9109330.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(18129..20111)
                     /locus_tag="ACG8VH_000752"
     CDS             complement(18129..20111)
                     /locus_tag="ACG8VH_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9109331.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(20125..21021)
                     /gene="murQ"
                     /locus_tag="ACG8VH_000753"
     CDS             complement(20125..21021)
                     /gene="murQ"
                     /locus_tag="ACG8VH_000753"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9109332.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(21024..22085)
                     /locus_tag="ACG8VH_000754"
     CDS             complement(21024..22085)
                     /locus_tag="ACG8VH_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9109333.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22296..22823)
                     /locus_tag="ACG8VH_000755"
     CDS             complement(22296..22823)
                     /locus_tag="ACG8VH_000755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9109334.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(22890..24392)
                     /gene="eat(A)"
                     /locus_tag="ACG8VH_000756"
     CDS             complement(22890..24392)
                     /gene="eat(A)"
                     /locus_tag="ACG8VH_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9109335.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(24650..25771)
                     /locus_tag="ACG8VH_000757"
     CDS             complement(24650..25771)
                     /locus_tag="ACG8VH_000757"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9109336.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            25897..26838
                     /locus_tag="ACG8VH_000758"
     CDS             25897..26838
                     /locus_tag="ACG8VH_000758"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9109337.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26988..27884)
                     /locus_tag="ACG8VH_000759"
     CDS             complement(26988..27884)
                     /locus_tag="ACG8VH_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9109338.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            28153..29493
                     /locus_tag="ACG8VH_000760"
     CDS             28153..29493
                     /locus_tag="ACG8VH_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9109339.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            29711..30154
                     /locus_tag="ACG8VH_000761"
     CDS             29711..30154
                     /locus_tag="ACG8VH_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9109340.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(30658..32076)
                     /gene="nhaC"
                     /locus_tag="ACG8VH_000762"
     CDS             complement(30658..32076)
                     /gene="nhaC"
                     /locus_tag="ACG8VH_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9109341.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(32479..33702)
                     /locus_tag="ACG8VH_000763"
     CDS             complement(32479..33702)
                     /locus_tag="ACG8VH_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9109342.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(33733..34572)
                     /locus_tag="ACG8VH_000764"
     CDS             complement(33733..34572)
                     /locus_tag="ACG8VH_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9109343.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(34589..35269)
                     /locus_tag="ACG8VH_000765"
     CDS             complement(34589..35269)
                     /locus_tag="ACG8VH_000765"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9109344.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(35271..36344)
                     /locus_tag="ACG8VH_000766"
     CDS             complement(35271..36344)
                     /locus_tag="ACG8VH_000766"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9109345.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(36400..36638)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(36681..36914)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(36991..37662)
                     /locus_tag="ACG8VH_000767"
     CDS             complement(36991..37662)
                     /locus_tag="ACG8VH_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9109346.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(37749..40397)
                     /gene="mgtA"
                     /locus_tag="ACG8VH_000768"
     CDS             complement(37749..40397)
                     /gene="mgtA"
                     /locus_tag="ACG8VH_000768"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9109347.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(40739..40913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            41086..41523
                     /gene="ndk"
                     /locus_tag="ACG8VH_000769"
     CDS             41086..41523
                     /gene="ndk"
                     /locus_tag="ACG8VH_000769"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9109348.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(41545..42411)
                     /locus_tag="ACG8VH_000770"
     CDS             complement(41545..42411)
                     /locus_tag="ACG8VH_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9109349.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(42571..44664)
                     /locus_tag="ACG8VH_000771"
     CDS             complement(42571..44664)
                     /locus_tag="ACG8VH_000771"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9109350.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(45413..46036)
                     /locus_tag="ACG8VH_000772"
     CDS             complement(45413..46036)
                     /locus_tag="ACG8VH_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109351.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(46195..47592)
                     /locus_tag="ACG8VH_000773"
     CDS             complement(46195..47592)
                     /locus_tag="ACG8VH_000773"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9109352.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(47624..48595)
                     /locus_tag="ACG8VH_000774"
     CDS             complement(47624..48595)
                     /locus_tag="ACG8VH_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9109353.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            48969..49394
                     /locus_tag="ACG8VH_000775"
     CDS             48969..49394
                     /locus_tag="ACG8VH_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109354.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        14360 a        10386 c         8798 g        16308 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgtta atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    13201 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    13261 agacactatt tttaaatatt tttcgttatt tttttatcac aaaaattatc ttacttattg
    13321 taaggtaaag ctttttttaa tttattgccc gataatatat agaaataagg tctagttact
    13381 gtttttttta aatgattaat aaataacaac ttctttctat gaaattgcaa agcattcatc
    13441 ttcttttccg ctaacgatat atccgtcagt gcttggtaca ctttactaaa aagtatgaga
    13501 ttgccgatat ctggataggt gtgctatact acagaaaaag atatacaatc tgccaccggg
    13561 taggataaag aggatgatca ttccattagg tctttgaagg gatgattttc ctctttttgt
    13621 gtattttttt atagttaagg aggaatgtaa gtatggcatg gatggaatta atcatagcag
    13681 gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt tctaaaatca
    13741 attattcttt ttacacagtg attggcatga ttgctagttt ttactttttg tcaaaggcaa
    13801 tcaaaagttt gccgatgagt ctctcttatc ctatctggac agggattggc gcagttggtt
    13861 cggtcataat tggtgtggtc ttgtttcatg ataagctgaa tctttccact tggttctttg
    13921 tggggatgct actcatcagt atcattggca tcaagattac tagcggccac tagacacttg
    13981 gaataagtac tgatcagaaa aaataagcct ttttatttaa tattcaattt ttttatgaaa
    14041 aaataagaaa taagtccaat gacctagcga cttctccact catcaggtgt atgcttgtac
    14101 tatctataga aaaggagtga atatttttga aaagtctttc ggtccaagaa ttatccaaga
    14161 actttggtca caaacaagcc ctcagcgatg tcagttttag cttatcccct ggcaaaatcg
    14221 ttggattagt aggtcctaat ggcgctggaa aaacaacgat catgaaagct attttaggac
    14281 tgatccatta ctcagatgga acaatctgta ttggtgaaaa tgagatttct ccctctagtc
    14341 ataaagggtt ggaacaagta ggagcattga tcgaataccc gggtatctat ccattcttaa
    14401 caggctatga tcatctgaag ttattctctg aaaccaacga tgtatctgct atcgacacta
    14461 tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc tactcgttgg
    14521 gaatgaaaca aaaattggga attgctcttg ccttactgaa ccatccgtct atcgttattt
    14581 tagatgagcc gatgaacggt ttggatccac aagcaacaag agatgtgcga aaaatcatta
    14641 ccgacttggc tactcagggc acctctttct tgatctccag tcacttactc agcgagcttg
    14701 aaaaaatggt cgatgagctg atcctgatca atcaaggaaa gatcatcaaa caatgtacaa
    14761 tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca aatgatctag
    14821 ctgctcgaac gatcttgaca gaagctggat atactttgga agatcctgat aaaatcaagc
    14881 ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca gctattgtct gcacaaaata
    14941 tccatgtgac agacgtccag catctgacga atgatttgga atcttctcta ttggaaattc
    15001 tggattcagt aaagggggaa gaagaaaaat gacattgctt gtaaaacaag aattatttaa
    15061 attgatcaag aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt tgctcatagg
    15121 aacagcactt ctagccaaaa aatacacgac gatcattgat ccagttgaaa tgactgctca
    15181 gctttttagt gccacatctt ggattgtctt tatcatgatt gcagctgcaa gcacgatcat
    15241 ttcgatggaa gcacaatatg gtacattgaa aaatctcctt tatcgaaagt attctcgagg
    15301 agaaatatta gtaagcaaat ggatcacatt agttatttat tctgtttatt tatacctttt
    15361 agctattatt gtcactgtgt tgatgaaact gattcttttc ccttcgattt ccttcacgga
    15421 gaaggtttct actggtcaaa cactgatcca aagcttgttc acttatactt tgggtagtta
    15481 tatcggttta tggctgattt taagtttagt gttgatgatt gcttgcttta tcaatagttc
    15541 cggcgcatcc atctcagcag gaatcgtctt ttattttgct tcgtcaatca tatctggtat
    15601 ccttattgca ctcatccaaa aatgggagtg gatcaaatgg aatccgatca gtatgttgaa
    15661 tctgcaaaac caaattggaa acgaagagat catgaagcaa ttgactcatc tttctactaa
    15721 tcaaatgcta ttcggtaatt tagcttacat cgttttgttc ttagcattag gatatctcgt
    15781 atttaagcga aaaaatgtat gatgactcat caaaaaagag gctgtgaaag agacgttcag
    15841 ccttattgcc gattggctgt ctcttaaaca ccgtttattc tgaaaaagga gctgtgacaa
    15901 catttgtgtc acagctcctt tcctagtatg tttttgaata aacgacaggc tcacaacctc
    15961 tctttatact tcttttatac ttctatacgt gaagcatccc agtggcaaat aaaaccattg
    16021 caatcactcc gaccatcgtc acacctataa gtaaaatctt atctaagtgg atatgttgta
    16081 caggctcgtt ttgctcctta cgtgctttga ggtaaaaata aatgcctggc aagaaaagaa
    16141 caagaacaag cattacttca ttaaaggcta cgactagcat agcccatact tgaaaggcac
    16201 ttgctaaaag ccctactaaa aattggaccc attctttccg ctgataagaa agtttcattt
    16261 ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt gtgtaagcaa
    16321 agttataagc actgtcagtg aataataatg aaagtaaaaa gacattcgta cataacgtcg
    16381 tcaatacgat agcataagtt ggtgccccat ttttgttgag ttttccccat gctttaggca
    16441 aaagtccgtc ttcagccatg tttcccattg tttcaatcgg taagatcgtc catgagagcc
    16501 aaacaccaac taaagaaatg atcaaaccgc cgttgatcac gactgctccc catttaccaa
    16561 ccatcatttc aaacacatag cccatagcag gctgcgcctt gatcgtttgc agctgattca
    16621 tcgtcattgt cccatatgga agcatggaaa ccaagacgta aataataatc aagccgaata
    16681 tccctagaat cgttgctttc ccaacatctg attgtttttt tgctcgtgaa cctacgacag
    16741 aggctccttc aataccaaca aatacccaaa gcatcaccat gatgcaactc aatacttgac
    16801 tggaaaacgg gatcggctgt ccattcgcat tcaatataat gtcgctagaa aagttatcaa
    16861 tgaattgttg gatcgaaaac gtgctgatag ctactacaat aaataaaaat aatggcacta
    16921 atttacagaa tgtgacgata gcattgacga aactagcatt ttcgactccc cgattcacta
    16981 gccatgcata tccccataaa aagactgtac tcatcacgat cgatgccaga ttttgcccac
    17041 cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa caatgttgca aaagctacgt
    17101 tgcctaacca gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg aaacggccaa
    17161 aaccttcttc tgcgtaacta aagatcccgc cttttaaatc tggacgtttt ttattgatat
    17221 tattgatcga caaggagagc atcaacattg ctgttcctac gagtacccaa ctgaaaatag
    17281 ctgggccgcc agccgatccg gagatgtccg tcataatacc aaatacgcca cctccaatcg
    17341 ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat tccttttttc tgttgctctg
    17401 tatccatgta agcatcctcc aaaataaaac cgcttttatc aaaaagtata acaaaccttt
    17461 tttattttta cttaattaat aaaaacttta atagaaatat aattctaccg gcttttttct
    17521 cattatacaa gcaaagcgcc tagtcccttt ccaaattaca ccaccaaaaa acttaaaaaa
    17581 gatgctatac taaaaggaaa gaattggagg atgatccatg aacattttat atatatcgat
    17641 ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa tatgctgaaa aaatgcatgc
    17701 agaagatcct gtcaatcctg aagttacttt aaaagaaatc catgaaaata gtgattttac
    17761 gaaagaaaca gaaccatttt ttactttcgt tcctacttat ttagacggcg gaaatggttt
    17821 agataacggt gatacagaaa tcttgaccga aacgatgcgt gaatatctgg aacatgaaga
    17881 caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat aaaaacttca ataatcagta
    17941 ttgtctaaca gccaaacaat atgcacaaac atttggcttt cctttcttgg cagattatga
    18001 attacgggga acaccatctg atgtgacacg aatttatcag attttagcag aaaacaataa
    18061 ataataaaaa agaagaagct gaaaaataga tttcagtttc ttctttcgtt tctattgttt
    18121 ctatcatatt aagctttgac atccatcaca ggtgcttttg cttttgcttt acctgttttt
    18181 tccaagacaa agttttttac acgatccata ttggttacca agacgatcat ggatgtttct
    18241 ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta caagcgtatc aggtgtcact
    18301 tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc ctaagtcaac tgtgttgatt
    18361 cccatatgaa gcaagatttc taaaccattt ggcgttgtga tcccaatagc gtgtttcgtt
    18421 gggaagacag agctgactac cccagtaact ggagaataga tatgtccgtt ttctggttct
    18481 acaaagtagc cttcacccat catcttttgt gcaaaaactg gatcacttgc tttatctaaa
    18541 gaaacaactg ttccgtcagc aactgatgag aggatagtca cactttcagc tgaagtttga
    18601 ggtactactt tttcaggcac ttcatttact gtggctgttt cagtcgctaa ttcttctttt
    18661 tctaaacggt ctttaggaat accgaagaag aatgtagcaa cgaagccacc agcataagct
    18721 gccaataatc caaggatgta gcctaaccat ttaccgtcag agatcaatgg gatcaaagcg
    18781 gctcctgaag gtccaatagc gatagccccg acatttccga tcattccgat cactgccccg
    18841 ccgatacctc caccgataca agcagtaata aatgggcgtc ctagaggtaa tgttactccg
    18901 taaatcaatg gttcgccgat tcctaggatc ccgactggta aagctccttt gatcatttct
    18961 gttaattctt tatctttttt gcagcgaatc cataatgcca atgctgcacc cacttgtcct
    19021 gcaccagcca tcgcaagaat tggaagcaat agcgtacgac cagtttcatc aatcatttgc
    19081 acatggattg gagtcaaaat ttgatgcagt ccgaacataa ccatcggcaa gaataatgtg
    19141 cctagaacga agccggcaaa tgctccacca acgccaagta cccagttgat cccaccgacc
    19201 atgccatcag aaatgaaacc tgctaaaggc atgatgaaga aaatttcaat cagaccgatg
    19261 gcaatcaatg aaagagttgg tgtaacgatg atatctaccg catcaggtac tactttatgc
    19321 agttttttct caacgattga caatagccaa acagcgaaga tgacacctag gatcccacct
    19381 tgtccagctg caagagcttc accagtaaat aagtttttca gtggtgcttc cggattcatc
    19441 cctgtcagca tcacaacggc cccgataact ccaccaagag ttggtgttgc accaaaaact
    19501 tgtgcagcat tgatacctgt atagataact aggtagctaa acatcccgtt tttcaagatg
    19561 ttcatcacat cgatatattg ttgccatgtt gctgcatcaa gtgttccggc tgtgactaag
    19621 tttgctaaaa ctgcggcaat ccctgccacg atcccagtac cgacaaatgc aggaatcatt
    19681 ggcacaaaga tattagaaat gtcttttaat accgatttga ttttagatgg tttttgtttc
    19741 gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt tccagatgct
    19801 gatgaatttg ctgtagctgt acttacagaa gaaagttttt ctcctaattt cacgcctgcc
    19861 aaatcaacca tttgctgtgc tactttgttc actttcccag gtccgatgat gacttgaagc
    19921 tgttcatctt ctactacacc aagcactcct ggaatcgctt ttaattcaga gacatttaca
    19981 agatcatcat tgatgatctt catacgcaca cgagtcatgc agtggacaat gctttctaca
    20041 ttgcccatgc cgcctgcccc tttataaatt tgttttgcca gttcttccaa agtcagttcg
    20101 ttttttgcca tggtcttttc cccctcataa ggtccctttt acaaatccgt ttgcttcagc
    20161 taattttgct tgagcttcct ctttatcact atttgtcaaa atcatgacga tagccagctt
    20221 aacattttga tctgcttctt caaagtactt ttcagctgtt tcgtaatcag cttctgtcgc
    20281 ttgcatgatg attcgttttg agcgttcgat tagtttttca tttgttggtt tgacatcgac
    20341 catcaagtta ttgaatactt tacctatacc aatcatactg atagtagaaa tcatattcaa
    20401 gataagtttt tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa attctggacc
    20461 tgcatcgact tcgattggga aatccgcgtg tttactgatt tcggcttgtt tattgcaaga
    20521 aatcgttcct gtgacagcac cgatcgattt tgcgtaatct aatccaccga tcacatatgg
    20581 tgtacgaccg ctagctgcga taccaataac tatatcattt tttgtcaaat tcagcttgat
    20641 tagatcttct tgccctagct cataagaatc ttcggcccct tcgacagcta cagtcattgc
    20701 ttcttctcca ccagcaatta gacctatgac catttccggt tctacaccga atgtcggtac
    20761 gcattcagca gcatctaaca cacctagtct tccacttgtt ccggctccca tataaatcag
    20821 acgtccacct tttttaaagg actcaatcgt gttagcaatc actggttcga tacttgtcag
    20881 ttctttttca accgcagcgg ccactttctg atcttctttg ttcattagtt gaacggcttg
    20941 agaaatactc atctcatcta atccaaaagt ttcttggttt ctgcgttccg ttgttaggtt
    21001 ctctaaattg attttgctca tttttatctt ccttcttttc ttataaattg atagttcata
    21061 ccagctttga tttggttgat caatggtagc tcttctttga taactttagc tactacattc
    21121 actttttcat ccgctggcaa atcatatttg gttaattgga tctcacccat atagcgcaaa
    21181 tacttttcgt tatctaaagt aatagaccct ttcatacgcg tagctgtatt tcttgctggg
    21241 ataggcggaa tctctcgaaa acgtgcatct acgcttcgta tgacatctct tgcatcgtct
    21301 tgccgattcg tatgcttccc taatatatat tcgtaaaatt cttcatctac aggttccgta
    21361 tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac gttttctgac
    21421 agacctgcat ctccgatgta aacaatatcc gtgttacttg ctaatagatc tagagcgcct
    21481 gcaagaggat ggatccctcg atgttcttcc aaagttggca atccttgata tagtgggcct
    21541 ctcaactctg tatctcctgg tacaaaacct tggacaagga gaccttgaat tttgagccat
    21601 tgattttttt tctgatacca ttctttgtcc agccctgttt cgggacgtgg ataataatta
    21661 tgccaagctt caatctgtga gaaatcagct tctgcttctt tcaattcatc gatatcttta
    21721 ggcgtgatcg tgctagcatt caaactgatt ttcaattgat gtgaccagtc ggcaatctgc
    21781 tgattggaaa tatgataatc cattcttaaa cctgttacac caattgcttt caaaggcgtt
    21841 agatcttcta atgaaaaacc cgctcgttcc aatgcctctc ctgaaatatc gaccatcaat
    21901 tctagtttat tctttttcgc ttcgcttcct agttcgatca atcgcttttg atatgccgaa
    21961 acatcatctt ctggtatatg catggaagtg aaaataccga caaagccatt ggatgccatt
    22021 tggcggatat actgtttctt ctcttctgac aaagaatcgt tcataaatat tgaaaagcca
    22081 aacatattat ccctcttttt ctgtatccgt cttcatttct atgactagag tatatatcta
    22141 aagaaacaaa atttcaatac ttttattaaa ataattaaat ttcatttcta aaggttgagt
    22201 ttatattatc aaaagaattg tcataaccta aaaaaggcac tgctaagcaa aagcagtgcc
    22261 ttgaaatatt ttatttaaat aacacaaaaa cataattact cataagaaac caattctgta
    22321 ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata agcattgatc
    22381 actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc ttcgatgcat
    22441 tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc atttgggaat
    22501 cgtacagcga acgctaaacg tgctttttga aagttctcat ctttgcttgg cagtccatca
    22561 taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa gctgagacga
    22621 tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat gattttcgtg
    22681 attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat ctgtcctttt
    22741 tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca agttgtttct
    22801 ccgtgtctcg ttacataaag catgtgattc tctccttcgt tggcaaagtt ccccttaacc
    22861 ataccttgtt gttcttgaga ttacaagggt tagattctct caagagaaat aatctcagta
    22921 gatactttat ccaggaaggt ttgatcatgt tccactagta acatggcagg tttcacgttc
    22981 aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa tggttcatcc
    23041 catgtatata gttcagctgg ctgtgataaa gatttagcca attccacttt tttccgttgg
    23101 cccatactca tctgctcgat cttgttatga aaaacatctc tttccatccc aagctttcgg
    23161 aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc cgccaacgtt
    23221 cctcgattgt cttcataatt ttggcttgca taactaatgc tcagatgttt tggctggtat
    23281 ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat ggatgacttt
    23341 cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt aaaatgaatt
    23401 ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa aagtcgctta
    23461 tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc ttttaatagt
    23521 ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga tcgtttcatc
    23581 gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc agtatcgatg
    23641 aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc ggctttttca
    23701 gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg agccatttca
    23761 aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg ataaagttcc
    23821 agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa tccccggtca
    23881 tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac ttgttttcta
    23941 ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt gtcatccaca
    24001 aataaaagtg ccagtaggac ttttgtcttc tctccccccg atagtgtgct gaattctctc
    24061 cataagattt caggatctgt ttgcatcaat tggagctctc tttcgatttc ccatatctca
    24121 aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt tgtctgttgc
    24181 gggaaatagg caaattcttg ctgatggatt acttgccctt gataaggtag tttgttttgt
    24241 agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc tagtttccat
    24301 tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct gtcgtagccg
    24361 aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa aaaagaccag
    24421 tcctattttc agaactggct aaagaggatg caagggttgg atacacacaa aaagacccat
    24481 acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gcttccttgt ctccacaagg
    24541 tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg atccctcact
    24601 ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac tacttcggga
    24661 ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct aatgtttgcc
    24721 tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag acaattggga
    24781 gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg attattggtt
    24841 ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg atcgtttgct
    24901 gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt ttttccaatc
    24961 cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac aaaaaaaggg
    25021 ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc acctcccaga
    25081 aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct agcgtcgtat
    25141 ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct gtatatggat
    25201 tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt cttcgatcaa
    25261 aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct aaagaagcac
    25321 ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct aaagtgacaa
    25381 taatttgttc tacttcttga agcagtgcat ctttaataag ttctcctaag gcataactgg
    25441 aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc aatgcttgtg
    25501 cagattcgat gactgcggtg ttttttcctt gccaagttgt caataaatag ctgatttttc
    25561 gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccctg agcgcgtaat
    25621 agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt tttcttcctt
    25681 tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa gcacttcctt
    25741 taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt cttaagtaag
    25801 acactttttt tcattttttt caaacaaaac aatgctattt tcttccattt ttctatatac
    25861 taaaagggaa ctcattgaat aaaggagaga aaaaacatga aaatcgcaat agctggggcc
    25921 ggagcaatgg gcagccgaat cggattgatg ctacatcaaa gcggaaatga ggtcctcttg
    25981 atcgatcgtt ggcctgctca cattgaagcc attcgtacaa atgggttgat tgctgacttt
    26041 aacggtaaag aagtggtcgc aaaactgcca atctattcac cagaagaaat catcgaaagc
    26101 aacgaacacg tggacttgat cgtagcatta acaaaagcca atcaattaga tgatatgttt
    26161 tgttcgatcc aatccattat tactgacaat acttatgttt tgtgtttact aaatggatta
    26221 ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa atatcttatt tgggatcacg
    26281 atgtggacag ctggtctagc tggtcccgga aaagtcacat tgttaggtga tggagaaatc
    26341 gaactagaga acttggaacc agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt
    26401 caagaagcaa atttaaatcc gatctatagc cataacgttc gttattccat ttggcgaaaa
    26461 gcttgtgtaa atggaacatt gaatggttta tgcacgattt tggattgtaa tattgctgaa
    26521 ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa tcgtatctga atttgcttct
    26581 atcgcagcaa aagaaggaat catactagat caagaagaag tgtaccaaca tattgcttct
    26641 acttatgatc ctgacaacat cgggttgcat tacccatcta tgtaccaaga cctgatcaaa
    26701 aaccatcgtt tgactgaaat cgactacatc aatggtgcca tttggcgaaa aggacaaaaa
    26761 tatgatattg ctacaccata ttgtgccttt ttgacccaat tagtccatgc aaaagaagga
    26821 atccttggcg cagaataaaa ggctctctga tgagaatcag ctgaatcagc agctggttct
    26881 ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat gccataagaa
    26941 ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt aggtttttta tttgttttca
    27001 acagaggaga ggtcatattc gtattgctca taaatagtgg aatcgttagt gataaatcgt
    27061 tttgcccaat tttctgcatc ataaaattcc aatagctctg ataggattgg cttaaatggt
    27121 gtatgcagaa attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa aatattttca
    27181 ggacgataaa aatgataatg agcaatcgct tgattatccg tcactagttt cttttgaaaa
    27241 tacatggatt caaatgtccg caaagtcact ccttgctgac cctcccgcaa cagttccaga
    27301 atcccgcgcg attcccctgt ttttttcagg tactcttcat aagtaagata tccaggttgt
    27361 tcattccctc gatgtggaag aatgaaatag ttggtcgtaa catctctttt tataaattcc
    27421 tgcttatagc tttctgctcg ttcttttctc ccattattcg ttgcaccaaa aaatagatcc
    27481 gtctttggtt cgctagaaga aaaattcata tgtttcgaat aaaatgaact attatgtttc
    27541 agcccaagtt cttttgcttc caaaaaatca aaatgataaa tctcatcgat tgccggttca
    27601 tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga agtgattcca aaaaaataac
    27661 acaatttttg cattggtaaa tgggcgcacg cgtcgagcca tctccatatc aaatccataa
    27721 tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc tagctgattt aatgaattcc
    27781 gactgtagta aatcttttcc ggaccaaaca tctaaagagc ggatggaagg caaaaaataa
    27841 agtgatttat tttttgcaac tagcagtacg tcatttttgt tcatcttttc cgcctcactt
    27901 tctctgtcac tttttcctat gatagcctgt ttaaatagta aaaagccagt aaaacgtttc
    27961 ctatatttgt atgaaaattt aacatttgta tgaaaatatg atgaaataaa tcattcttct
    28021 attaaaattc gcctaaaagt tttctgaata gttgtaattg tctgaaaatt tgttatcatt
    28081 agtcattgtt aaatattgct atttattctt aatataaatt ttgattgtga atttaaaaga
    28141 aagaggtcat ttatggaaga taaaaaattg aaaaaacaac ttagctccag acatatcacc
    28201 atgttagctt taggtggagc aatcggtgca ggtctattta aaggaagtgg cgaagcgatt
    28261 gggatcgccg gcccttctgt attgattgct tttttgattg gtgggctgat tttatatatc
    28321 gtgatgaaag gtctgggaaa aatcgtttta agcggtggcg acacgcatca cggattatct
    28381 ggtttgatac gcccttatct aggtgctcat tctgctgatt ttactgattg ggtctactgg
    28441 tctatgtgga ttatcaatat catcgctgaa gctgttgcag ctgcttcctt tttgcagttg
    28501 tggttcccgc acattccagc ctgggtattt gtttttatgt tggctgtttt aactacgatc
    28561 atcaatcttt attcggtacg gttattcgca gaaacagaat attggctggc ttttgctaaa
    28621 atcagtgtca ttatcttact gatcattttt gctacttatc ttgtcggtca gcaaatgttg
    28681 ggcacaggcg ttttccctac ccttcagcaa ttgactgacc atggtgggtt tactcctcat
    28741 ggtatgaagg gaatcatcag ttctttgctt gtagttatct attcttacgg cggttctgaa
    28801 ctgattgcta tcacggtaag cgaagcagat gatcctaaac gtgcgattcc taaagcaatc
    28861 aaaggtgtca tgggaagaat catttccttt tacatcattc ctttgttttt gcttttgatt
    28921 ctttttcctt ggaatacttt agcaggcacg aatgtcagtc cttttgtgat ggtatttgaa
    28981 aaaatgaata tcccatttgc agcggatatc gtcaattttg tgattgtcct tgctttgttc
    29041 tcatctatca attcgggtgt gtatgcatct tcaagaattt tgttcttccg tttgaaggat
    29101 cgaaaaggtt cctctagaaa actagctgtt ttaaacaagc atcaagtacc gcaacgtgct
    29161 gttttatttt gtaccagtgt tctttatctt ggtgtagctc tttcttattt tgtcggtgac
    29221 aaattatttg gctatctggc aggttccctt tcgtatacag tattattgat ctggatcatt
    29281 atcagtgcgg catcctttgt actggcacta agaaaaggaa cattatggga taaaggaatc
    29341 agcttttttg cattagctgt cttaggattg attttccttg gtatcctgtt cacgaactcc
    29401 attggggtaa ctgtattgac tggcctgctt tacctgttca tttatttcag ttatcaaaaa
    29461 aagaatgatg cgttcgtatt gacgaatgaa tgaaatcgat agaaaatttt attttattta
    29521 tgtggaatag atggagctat tgaagaaagg agttgtgaca aaagtcttgt tgcaactcct
    29581 ttttccgaat aaacagtctc atgacctctt ggtaaacggt gttcaaaaac cctctgtgca
    29641 aaataattga ttgttctcca tgctcatgac atgatagaag aaaggaatga aaaaaaggag
    29701 tttgttttaa atgaaaaagt attggaagtt tttgttgatg atcggtgttt caacggtagt
    29761 tatgtttggt atgatgtact tgaatgtcta tgcgctcgat catatcttct tcagtcgtac
    29821 acgagtattc atggcgttga tgatgggtgc agtcatggca gtcatcatgc tgttgttcat
    29881 gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc ttagccgcta gtcttcttgt
    29941 ttttgccggt tccttgttta tggtacgaag ccaaactact gtcaacgaca cagcttggat
    30001 gaaggccatg attcctcatc attctatcgc tatcctggca agtaaacgag ctgatttaaa
    30061 agatcctgaa gtaaaaaaat tagcggaaga aatcattcag gcacaagaag aagagatcga
    30121 caaaatgaaa aaaatgatca aagaaatgga atgaccatta tccgtccaaa taaaaaaaga
    30181 ggatgttcca gaagttatca gcttcaagaa ataagaagaa gctacttctg gaacgccgtg
    30241 aataaggggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata
    30301 gcttctccta tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg
    30361 tttattaaga ggttgtaaaa tcagcgttct gacctgagta ttagagagac aaatggaaaa
    30421 atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc
    30481 gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa
    30541 atggctttcc acatttttgt ataaatttgg cttaataccg agaaactggc ttttgaatac
    30601 cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac aatccctttt tatgttttta
    30661 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
    30721 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
    30781 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
    30841 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
    30901 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
    30961 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
    31021 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
    31081 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
    31141 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
    31201 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
    31261 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
    31321 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
    31381 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
    31441 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
    31501 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
    31561 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
    31621 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
    31681 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
    31741 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
    31801 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
    31861 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
    31921 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
    31981 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
    32041 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
    32101 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
    32161 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggaaccac
    32221 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
    32281 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
    32341 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
    32401 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
    32461 acacaagatt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
    32521 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
    32581 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
    32641 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
    32701 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
    32761 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
    32821 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
    32881 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
    32941 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
    33001 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
    33061 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
    33121 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
    33181 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
    33241 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
    33301 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
    33361 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
    33421 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
    33481 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
    33541 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
    33601 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
    33661 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
    33721 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
    33781 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
    33841 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
    33901 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
    33961 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
    34021 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
    34081 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
    34141 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
    34201 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
    34261 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
    34321 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
    34381 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
    34441 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
    34501 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
    34561 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
    34621 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
    34681 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
    34741 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
    34801 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
    34861 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
    34921 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
    34981 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
    35041 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
    35101 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
    35161 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
    35221 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
    35281 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
    35341 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
    35401 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
    35461 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
    35521 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
    35581 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
    35641 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
    35701 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
    35761 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
    35821 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
    35881 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
    35941 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
    36001 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
    36061 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
    36121 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
    36181 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
    36241 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
    36301 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
    36361 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
    36421 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
    36481 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
    36541 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
    36601 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
    36661 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
    36721 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
    36781 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
    36841 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
    36901 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
    36961 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
    37021 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
    37081 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
    37141 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
    37201 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
    37261 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
    37321 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
    37381 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
    37441 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
    37501 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
    37561 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
    37621 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
    37681 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
    37741 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
    37801 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
    37861 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
    37921 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
    37981 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
    38041 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
    38101 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
    38161 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
    38221 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
    38281 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
    38341 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
    38401 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
    38461 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
    38521 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
    38581 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
    38641 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
    38701 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
    38761 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
    38821 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
    38881 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
    38941 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
    39001 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
    39061 ttcagcattt cttcaatcgc accttttgtg atcatttgtg ttttaccagc tggatcagca
    39121 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
    39181 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
    39241 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
    39301 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
    39361 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
    39421 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
    39481 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
    39541 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
    39601 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
    39661 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
    39721 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
    39781 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
    39841 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
    39901 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
    39961 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
    40021 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
    40081 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
    40141 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
    40201 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
    40261 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
    40321 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
    40381 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
    40441 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
    40501 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
    40561 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
    40621 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    40681 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    40741 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    40801 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    40861 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    40921 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    40981 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    41041 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    41101 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    41161 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    41221 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    41281 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    41341 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    41401 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    41461 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    41521 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    41581 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    41641 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    41701 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    41761 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    41821 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    41881 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    41941 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    42001 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    42061 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    42121 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    42181 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    42241 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    42301 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    42361 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    42421 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    42481 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt
    42541 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    42601 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    42661 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    42721 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    42781 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    42841 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    42901 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    42961 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    43021 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    43081 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    43141 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    43201 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    43261 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    43321 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    43381 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    43441 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    43501 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    43561 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    43621 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    43681 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    43741 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    43801 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    43861 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    43921 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    43981 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    44041 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    44101 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    44161 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    44221 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    44281 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    44341 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    44401 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    44461 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    44521 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    44581 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    44641 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    44701 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    44761 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    44821 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    44881 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    44941 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    45001 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    45061 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    45121 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    45181 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    45241 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt
    45301 caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
    45361 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc
    45421 cataggattg atattgtcct tgtgatgact cattcgtaat aattgaggct tgattttctt
    45481 cacttcccgt tatcgtttgt ccactgccag aatctgaagg acttgttcct gtcgactcgt
    45541 ttttttgaga agagacagtt gttttcttga cttgttcatt tgtctttttc cattccgcta
    45601 aacgatcggc gaccactttt ttgtctactt tttcagaata agcggtgatc acttttccat
    45661 ttttcaaacg ggctactttg tcgccatcat gagtcaaaag caaaacggtc aaatgcttgt
    45721 ctttaaattg atattgtctg agagaaatcg cactaagtac gtcatcgata ttaataaatt
    45781 gattttcttc ttttcctttt ggcaaataaa tgtccaattc gcctttttcg taattggcag
    45841 ttattttata ctccgcccac caataagttg agaattcttt tttgaaatta tctactgatt
    45901 ctttgcttgc tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg
    45961 aaaatgctgc ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg
    46021 gtcttttttt cctcatgctt tcctccactc ctatttaatt ttgactattc tattatatac
    46081 aaaaaatgaa atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt
    46141 tcacaaaatg gtaagaatgg agaatttcac gcatttgctc tcaactattc tttcttaagc
    46201 ctcaaatccg tttttatctg atacgtcttt gaaccaatga cccgattttt tgattgttct
    46261 ttttccatct tgtgctaagt ctactgaaat aaagccatat cgattcttat atgcattggt
    46321 ccacgaccag ttatccatac atgtccacat atgatagcct aaacaattgg caccttcttg
    46381 aatggcttga tggacatatt tcagatgaga ttggacaaat tcgatccgat aatcatcgtg
    46441 gatcatgcca tcttctttga taaatcgtgc ttcgttttct acacccatac cgttttcaga
    46501 aataaagcag tcgatatttc cataatgatc tcgtacattg atcaaaatat catagattcc
    46561 ttgttcatag atctcccatc cgcgataagg attcatttta cgaccaggca tttcataatt
    46621 gtcgaaataa tcctcaggca ttggaccgtt tttagtatcg attggtgttt cttttgcttt
    46681 tacccggcgt ggctggtaat aattgatacc taaaagatct actgtatttt ctttgataac
    46741 cgtcaaatcc tctgcatgga tcacaggcat gagatcaagt tcttttacta tctcaatcaa
    46801 ttcacttgga aactcgcctt ttacagctgg atcaaggaat gaacgattga agaacgcatc
    46861 tgctatttgc gctgctttaa catcttcagg gtttgattcg tctcttggat agcttggtgt
    46921 cagattcaag atgataccaa taacaccacc ttgctccatt tcatggtaag atttgatggc
    46981 taacgctgaa gccaacgctt catgatagcc aacttggatc gcttttttca tatctgactc
    47041 ttctggataa tgccatcgat ataaataacc tgcttcgact ggaacgattg gttcattatg
    47101 agtaaaccat ttttttactt tcgaaccaaa caattcaaaa caacttttgg catatttttc
    47161 ataggcttca accgtttctc ggttcaacca tccgcctttt tcttgcagat acatcggcat
    47221 atcgaaatga tacagattca caaagggttc gataccgttt tcaatcaact catcaaaata
    47281 acgatgatag aaacgaacgg cttcttcgtt gattgttcca gattcatcag gtatcaaacg
    47341 gctccattgg atagatgttc gaaatgaatt atgcccagtc tgtttcatca actgtacatc
    47401 ctcttgatac ttcgtataaa cttgcgaggt tttttcaggt cctacttgat caaagaactt
    47461 ttgcggttct tttttgtacc aataatcaaa aatgttctct ccttttccat cgcctgaaat
    47521 ccttccttct gtttgtgggc cacttgctgc agaaccccac caaaaatttg ctggaaattg
    47581 atattttttc atcaaagcac ctctttttct tttttagttc gttttagttg attccatgta
    47641 tgatattttc catcacaatc actttttcat tttcttcctc ttcttttttg attcgtttta
    47701 acagctgttc tgctgctcgt tccccgattt tatctgttgg tggcgtgatc gtgattttcg
    47761 gaaggatact cagatcgttt ttatctaaat aatcatagtg actgaacgca atatccttcc
    47821 cgattctgac accttcttga ttgaaacatt cgatggcacc aatcgacata ttgtaattgc
    47881 tgacaaaaat agcagttgcc cctttttcta ataatgcttt tgccccgtaa tagccgcttt
    47941 ttctagaata gtctcctgta aagacctcta attggatcga tggatcattt tggaccgctt
    48001 ctttggctcc tgctaaacgt tctcttgcta cgtagtctgt ttgagtggca gcaataaatc
    48061 cgattttttt atgtccttgt tcaccatgat gagcgatcac ttttgctaca ctttctcgat
    48121 cattcactaa tattgtatca atacctgctg gtccttctgg acaattcaat gcgataactg
    48181 gaatactcat ttttgtgagc atttcaaatc cagaccactc ttcttcggat aagaagacaa
    48241 tcagcccatc gatcacatgc ttcatcaggt attcaatttt ttgttcaatt ttttctggct
    48301 gatcggcaaa cccgcttaat aataagctat acccattttc ttcagctact ctttcgatcc
    48361 ctgagacaac ttccgaccca aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga
    48421 agcttcgatt attcttcaac cctttagcaa tgatgttttc tttatagtcc aacgctgaaa
    48481 tcgcttctgc tatttttttc atgttctttt ccttcagttg ctgaccattc aagtaacggg
    48541 aaactgttcc cactgaaaca ccagccatct tagccacatc acggatcgat gccatacatt
    48601 ctcactcctg ccagttacat ttgatttctc tttcttgttg ttcgattatg atgaccgcat
    48661 tctttgcata gtgttgcttg tcatcaatat ccatataact aaagtcttca gtagagcaac
    48721 ttcctctcac tattaattat acttaaacaa gtatttgaac cggttcattt ttaatatagc
    48781 atttttttaa aagattgcaa cactttttta aaacgctttc tgaaagtggc ggattttttg
    48841 cctttttaaa attttattct aaaatttact ctatttgtgg gtgttttgtg cgtttttctc
    48901 ttccatccgt ttgaagtttt acattccaat ggtataattt ctttatccta tcaaatgggg
    48961 gaagtatttt gaagaaatta atcgtactat ttattttgtc tgcgggactt ttgacattga
    49021 ctggctgtga tttgtttggc ggctctgacc aatcctcgga cgaaacaacc gattcttctg
    49081 tcttaaaagc ggaaaaagca gataccacca acagtgtata tacgctaaaa gtacgtaaag
    49141 atatggattt atccgcggga cagttgacat ttacagataa aaaaatggaa tggacacgca
    49201 cgtatcagac acaaggaact gattcttctg aatcatctga agaaaaaaca gtgctgacag
    49261 atatcaagat cactaccaaa gatgacgaat atattatttc aggaaaagaa aataaaaaag
    49321 aaacaacagt catatttaca aaaatcggta attaccgttt gaaggatgag gatggcaaca
    49381 tctacagtct atagtgccgt ctgttggatc ttcttttgtt tctccccttc ttttctgcta
    49441 tagtaaagaa gcaagatgat tgtaaaggag acgaaatgga tgatcgatag tcctgattat
    49501 tgtacatgta ataacaacaa aaatatccat acagaattta atgaccaagg aagcttgagc
    49561 atatgcgaca actgtcacaa actagtcaaa gattcattta aaacatttga atcttatcct
    49621 gttcaaaatt atgtaaaaag tagaagaaga taaacaaatc caagcaaaac tcactatggc
    49681 tgatttttat gaacttcaaa ctcagcttct tcctttgatc aagcttcatc agtaaaacaa
    49741 acagtggatc atttcagttt acaaatcaaa gagtaaagat ttatttattt caaatatttg
    49801 aatataaaat aaccaaacga tagaaaggct tattctacat cacgtagtaa tt
//
LOCUS       DAZKCC010000013        46563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000013 DAZKCC010000000
VERSION     DAZKCC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.013"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..930
                     /locus_tag="ACG8VH_000776"
     CDS             49..930
                     /locus_tag="ACG8VH_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9109355.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            943..2553
                     /locus_tag="ACG8VH_000777"
     CDS             943..2553
                     /locus_tag="ACG8VH_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109356.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2630..3289)
                     /locus_tag="ACG8VH_000778"
     CDS             complement(2630..3289)
                     /locus_tag="ACG8VH_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9109357.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3572..4237)
                     /locus_tag="ACG8VH_000779"
     CDS             complement(3572..4237)
                     /locus_tag="ACG8VH_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP9109358.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4384..4989)
                     /gene="dhaL"
                     /locus_tag="ACG8VH_000780"
     CDS             complement(4384..4989)
                     /gene="dhaL"
                     /locus_tag="ACG8VH_000780"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9109359.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5017..6027)
                     /gene="dhaK"
                     /locus_tag="ACG8VH_000781"
     CDS             complement(5017..6027)
                     /gene="dhaK"
                     /locus_tag="ACG8VH_000781"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9109360.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6002..6397)
                     /gene="dhaM"
                     /locus_tag="ACG8VH_000782"
     CDS             complement(6002..6397)
                     /gene="dhaM"
                     /locus_tag="ACG8VH_000782"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9109361.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6551..6871
                     /locus_tag="ACG8VH_000783"
     CDS             6551..6871
                     /locus_tag="ACG8VH_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9109362.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6983..7840)
                     /locus_tag="ACG8VH_000784"
     CDS             complement(6983..7840)
                     /locus_tag="ACG8VH_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9109363.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7948..9252)
                     /locus_tag="ACG8VH_000785"
     CDS             complement(7948..9252)
                     /locus_tag="ACG8VH_000785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9109364.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9249..9758)
                     /locus_tag="ACG8VH_000786"
     CDS             complement(9249..9758)
                     /locus_tag="ACG8VH_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109365.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9933..12068)
                     /locus_tag="ACG8VH_000787"
     CDS             complement(9933..12068)
                     /locus_tag="ACG8VH_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9109366.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12151..13806)
                     /locus_tag="ACG8VH_000788"
     CDS             complement(12151..13806)
                     /locus_tag="ACG8VH_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9109367.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13808..14122)
                     /locus_tag="ACG8VH_000789"
     CDS             complement(13808..14122)
                     /locus_tag="ACG8VH_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9109368.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14292..14552
                     /locus_tag="ACG8VH_000790"
     CDS             14292..14552
                     /locus_tag="ACG8VH_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP9109369.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14566..14880
                     /locus_tag="ACG8VH_000791"
     CDS             14566..14880
                     /locus_tag="ACG8VH_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP9109370.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15610..16164)
                     /locus_tag="ACG8VH_000792"
     CDS             complement(15610..16164)
                     /locus_tag="ACG8VH_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9109371.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16290..18219)
                     /locus_tag="ACG8VH_000793"
                     /pseudo
     CDS             complement(16290..18219)
                     /locus_tag="ACG8VH_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18525..19241)
                     /locus_tag="ACG8VH_000794"
     CDS             complement(18525..19241)
                     /locus_tag="ACG8VH_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9109372.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19242..21065)
                     /gene="glpO"
                     /locus_tag="ACG8VH_000795"
     CDS             complement(19242..21065)
                     /gene="glpO"
                     /locus_tag="ACG8VH_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP9109373.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21079..22575)
                     /gene="glpK"
                     /locus_tag="ACG8VH_000796"
     CDS             complement(21079..22575)
                     /gene="glpK"
                     /locus_tag="ACG8VH_000796"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9109374.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22711..24171)
                     /locus_tag="ACG8VH_000797"
     CDS             complement(22711..24171)
                     /locus_tag="ACG8VH_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109375.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24161..25456)
                     /locus_tag="ACG8VH_000798"
     CDS             complement(24161..25456)
                     /locus_tag="ACG8VH_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9109376.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25788..26465)
                     /locus_tag="ACG8VH_000799"
     CDS             complement(25788..26465)
                     /locus_tag="ACG8VH_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP9109377.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26495..27586)
                     /locus_tag="ACG8VH_000800"
     CDS             complement(26495..27586)
                     /locus_tag="ACG8VH_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9109378.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27687..28121)
                     /locus_tag="ACG8VH_000801"
     CDS             complement(27687..28121)
                     /locus_tag="ACG8VH_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9109379.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28705..30162)
                     /locus_tag="ACG8VH_000802"
     CDS             complement(28705..30162)
                     /locus_tag="ACG8VH_000802"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9109380.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30159..32054)
                     /locus_tag="ACG8VH_000803"
     CDS             complement(30159..32054)
                     /locus_tag="ACG8VH_000803"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9109381.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32112..33887)
                     /locus_tag="ACG8VH_000804"
     CDS             complement(32112..33887)
                     /locus_tag="ACG8VH_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9109382.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33937..35433)
                     /locus_tag="ACG8VH_000805"
     CDS             complement(33937..35433)
                     /locus_tag="ACG8VH_000805"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9109383.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35627..37249)
                     /locus_tag="ACG8VH_000806"
     CDS             complement(35627..37249)
                     /locus_tag="ACG8VH_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9109384.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37577..38890)
                     /locus_tag="ACG8VH_000807"
     CDS             complement(37577..38890)
                     /locus_tag="ACG8VH_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9109385.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39135..39359)
                     /locus_tag="ACG8VH_000808"
     CDS             complement(39135..39359)
                     /locus_tag="ACG8VH_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109386.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39379..39801)
                     /locus_tag="ACG8VH_000809"
     CDS             complement(39379..39801)
                     /locus_tag="ACG8VH_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9109387.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39943..40653)
                     /locus_tag="ACG8VH_000810"
     CDS             complement(39943..40653)
                     /locus_tag="ACG8VH_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP9109388.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40671..41168)
                     /locus_tag="ACG8VH_000811"
     CDS             complement(40671..41168)
                     /locus_tag="ACG8VH_000811"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109389.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41205..41657)
                     /locus_tag="ACG8VH_000812"
     CDS             complement(41205..41657)
                     /locus_tag="ACG8VH_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9109390.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41792..42397)
                     /locus_tag="ACG8VH_000813"
     CDS             complement(41792..42397)
                     /locus_tag="ACG8VH_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9109391.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42847..43467)
                     /locus_tag="ACG8VH_000814"
     CDS             complement(42847..43467)
                     /locus_tag="ACG8VH_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9109392.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43469..44338)
                     /locus_tag="ACG8VH_000815"
     CDS             complement(43469..44338)
                     /locus_tag="ACG8VH_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9109393.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44361..44768)
                     /locus_tag="ACG8VH_000816"
     CDS             complement(44361..44768)
                     /locus_tag="ACG8VH_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9109394.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44787..46076)
                     /locus_tag="ACG8VH_000817"
     CDS             complement(44787..46076)
                     /locus_tag="ACG8VH_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9109395.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46134..>46563
                     /locus_tag="ACG8VH_000818"
     CDS             46134..>46563
                     /locus_tag="ACG8VH_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9109396.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        13115 a         9903 c         7986 g        15559 t
ORIGIN      
        1 atttaatctt gtttagtata tatttttaga aaaaggagtg gggtttttat ggcagaaatc
       61 gtcaaagtca cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa agatgtctct
      121 ttcaccgtac attcaggtga agtcgttgga tttatcggac caaatggttc tggaaaatcc
      181 acgacgattc ggacactttt agggattata aaaaaagacg gcggaacggc aacgattttt
      241 gggcaagatg tatgggaaga tagtttatct atccatgaac gtctttctta tgttccaggc
      301 gatatctctt tgtggggaaa tttgactgga ggagcgatca tcgatctctt catgaagctt
      361 catggaaaag ggagtgctca aaaaaaagat gaactgattc aacgtttctc tcttgatcct
      421 aagaaaaaag caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt gattgctgct
      481 ttggctgttg attcggatct ctatttattt gatgagccta cttctggttt agatcctttg
      541 atggaaacga ttttccaaga agaagttgaa aaattgaaag aacagggcaa atccatccta
      601 ctttcttctc atattctcag tgaagtcgag cgactagcgg ataaagtcgt gatcatacgc
      661 gaaggaacac tcgatgaaga aggaacactc gatgaattgc gtcatttgac tcgttcgacc
      721 atcactatag aaacagaagc agagaatagt cacttagcac aattgaacgg tgtccatgac
      781 tttgtgcaaa aaggtcacct cgctcaattt gctgctgata acgaagcttt cgatgtgatt
      841 ttagctgaag cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc gactcttgag
      901 gatttattca tgagacatta tgaaggatga ggaggaagcg aaatgactga taaattttca
      961 cgctggtcca cccttttttc tcaaaacctt agccgtgact ggaaaaagat cctgatatgg
     1021 ctagtcggtg tcactgcttt ttctggtggt tttgtccctg cgtttgaaga attgacttcc
     1081 ggacaaggat tagctggatt atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc
     1141 ggaccaactc cagcttcgaa tgctgctgag tatactttag gagcgatgta tgcaaatgaa
     1201 atgttgcttt tttgcagttt gtttgctgct gtgatctctg ttttacacgt ggtgagccaa
     1261 acgagaaaaa aagaagaact tggtctgatt gaacttattc gttcctttcg agtcggacgt
     1321 caagcaaact ctttagctgt catcgctgaa agtattctga tcaatttgct tttagcaata
     1381 tcgatcagct gtacgatgat ttttttccaa gcggattcga tcactgcaga aggtgcttgg
     1441 ctttttgggc tatctgtagg tagtatgggg attttgggga gtgccgttgc actgtttgct
     1501 gcacaactga tgccaagttc agccagttcg aatggtctgt ctttagcgtt tttaggtgga
     1561 ctttaccttc tacgagcaat aacagatgta gctgattcct ccctttcgat gtggaatcct
     1621 ttaggctggt cttatttgac ctatccattt acaactaaca actggattcc tttgctttat
     1681 ttgtttttgt tcagtctaat cttgataatc agttcatttg ttttagagga acatcgagat
     1741 ttgaacaccg gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta
     1801 tccttgcgtg gtttattatt ctatctaaat aaagggatga caatcagttg gcttatcact
     1861 tttggtttga tgggggcggc atacggctct atctacggga atatgcaaca gtttttagaa
     1921 agtaacgact taatgaaaca gatgtttatc caatctggcg tctcaattga aaaaaacttt
     1981 acaagcacga tcatggtagt cttgatttgt ctagctgcta ttttaccaat tgctagcatc
     2041 aatcgtttat ttacagaaga aacccacgga cgtatgaatc agctgttcgc aacaaaaatt
     2101 tctaggaacc aactttattg gaccaatatg ttactagcta ttctatttgc tatattggca
     2161 atcctgatta gttctggtgc actagggctt tctgctttgg cttcgatgaa ggatgcgaaa
     2221 gacatggatc tattgatgtt tcttaaagcc gggttcaatt tcctgcctgc cgttttagtg
     2281 tttatcggaa ttagtggctt tctattcgga tggcttcctc gaatcggtaa acttgcttat
     2341 atctacttag cttactcctt tgcactcaat tactttggcg gaattttgga tttaccagaa
     2401 tggttttcca aaacagctgt ccctagctgg gttccacgga tgccaaacga ttcttttgat
     2461 ctttccgtgt tccttacgtt atcatgtatc agccttgcac tgttcgtcct tggttctatt
     2521 ggttataggc gaagagactt actagaagga taataaaaaa gcatgtacaa tttcatgcta
     2581 gcctccaaat ctgggtcaaa aatccaacat ttggaggtca tttttttatt tacaagtctt
     2641 ctaactgctc tttatcaatg atccagactt ctcgattctt tctttttaca taaccattct
     2701 cctctaataa tttaaatttt ctagataaag tttcgggggt cgtaccgata aatgcagcaa
     2761 gttctttcat tttcattggc aggaaaaagt gatccttccc tgtcgcttta gctaagtcaa
     2821 gcagatagac agcgactcgt tcttctactt tttccatcat caaaaaagtt gtctgctgct
     2881 gtacttttgt taatttctca acgttgattg ctaaaagttt cagactaagg gcgggataat
     2941 gcagtaagag ttccgagaaa tctttttgtc tcaatatgca aatttccgtt ttttctagtg
     3001 cttcgccaaa taaagactca tttggaacgc ctagcaactg gctctctccc tcatatccgc
     3061 ccggctctat tactcgtaaa agctgttcct ttccgctagc agataattga tacactttca
     3121 tactgccgga ggccacgatc actaattgag gttctccatt cggcgcaaga atctgttctc
     3181 ctttttccac atagtgatgt ttgacaagtg cgtgtacttt ttcttgatct tgctgtacta
     3241 agtgattgaa caaaggaaca aatgaaacac ataaatgatc atgactcata atctctcctc
     3301 cttctaaaca gctatttcta ataaagaaac ataacttccc ttattccaac tgataatttt
     3361 cttttatcat agcataaacg tcaagcgaac cagataccag caaatagagt ggatttcttt
     3421 ttgtcccttg ctttattttc cttcttttga gaccttaagt aattgattcc tatcaatttt
     3481 ttaaatattt actttttgaa taatcaaaaa caaaccttca ctcgcaagcc gagagtaagg
     3541 tttgtttttc gcattgctta tttcgtgttg tctattgaaa tttttccgca cctagctttt
     3601 gatatagtgg tgctagttct tgtaaggttt cagcggttat ttgctcaacg tgttcgattt
     3661 cttgcggata gaagaatagt ttacccattt gaaaggttcg gtctctcatc gcttttttca
     3721 actcttcttg tttctttgta tcggacaaat agtcagacaa agctaagtgg tcgtcaagtt
     3781 catgttctgg ttgcggccat atgtaataat ccttgatatc gactgttttg ctccactctt
     3841 tcagtatcgc aagcaactgg ttatacgctg caatgctacc atatcgttct tcgcttttat
     3901 aatgttggta catcaaatag atttggtatt gtttttgatt caacaaaact gctagacagg
     3961 cattctcgtc ttgacgagat tcgcttcggt acgcgctcca aaaatgactg cgtaaattcc
     4021 agccattcgt ccagctttcg attttaggtt tgctcatgcc gtacatatca ggcaaaagtg
     4081 cagctgtctg gagctgaatc tctttccatt tttgccagtg ttctttatat tctttcttga
     4141 tttgttcaat cttatctgtt ggatggctct ctttgagctg tttgaatgcg tagattggtc
     4201 gatcaaacaa ctgataatct gattttccaa gttccattgt ttcgcctctt tccgttatct
     4261 tttgtattct acctgaattt tgcgatatgg acaaatagag gttgtgaaaa agctgttctg
     4321 catcaagtca taagaacagc caaacaaaaa actgcgatga gcatcaccgc agttttattg
     4381 aacttaaggt tgattgatca catctaaaag cgttgaaaat aaaatggcac tagaagcagc
     4441 tcctgggtcg atatgtccga ttgcacgctc tcctagatag gaagctcgtc cttttttagc
     4501 taccaagact ttggtgtgtt cttttgcctg gtcaatgatt tcttttgtta aattccctgc
     4561 cttcaatgcc tctgtgactg gcagccaaac atctaacatc gttttatcgt cagcttctgc
     4621 ctttcctcgg ctttgaatac ccgctgttcc ctgctcgatc acttcgccta atttttcttg
     4681 agaatcaata gaatcgacct cttttgtcgc tttcatcatg ttcatgaatg cagatccgta
     4741 taacggacca gaagccccac cgactttcga tatcatcgcc atagagaata ctttgaatgt
     4801 atcgctgatt gtttcaggat gatcggcttg aaaagcttct tcataagctg aaacccctct
     4861 tcccatgtta tttccatggt ctccatcgcc aatcggggta tctagatcac tgagatattg
     4921 tttgttctga ttgattgcct ctgcaaattg cgtgagccat tcttggattt cttgaacggt
     4981 tagtttcata ttatcaccct tttatcttga gattttttac catgaaatcg tctgtacatt
     5041 gctttctaaa gaatctttcc attgtgtctc cgtcaagtcg atcagtgtta gagaaagacc
     5101 ttgcatatct agtgaggtca taaaattacc gacttttcgg aaaactacat tgacgcctct
     5161 ttcttccaac agagctaaaa catcattcat gaacacgaac tgttccatca aaggcgtccc
     5221 gcccattccg ttgactagta cacctgcttc ttttggtaaa ttcgagtaat catctaaaat
     5281 cttagtaaca agttcctttg ccagtacttt agaaggctgc atcttttctc gacgatagcc
     5341 tggctcaccg tggataccaa caccatactc gatctcatct tctcctagtt cgaatcctgg
     5401 tttccctact tctggtacag ttgctgctcg aagcgctaga ccgatcgtct tagttgcctg
     5461 tacgaccttt tctcccagtt ctttcaactc agctagggaa gcaccgtttc ttgctgcatc
     5521 gcccacgatt ttgtgtacaa ggacagtacc tgcaactccg cgttttccag cagtatacgt
     5581 actattttca acggcgatat catcatctac gatcacactt tctacttcaa tatcgtccat
     5641 agcagctaat tcctttgcca tatcgaagtt caatgcatcc cctgtataat tcttaacgat
     5701 cagcaaaact ccttttccac tatcggcttc cttaatagcc gtctgaatct gatctggagt
     5761 tggagaagta aacacatcac caagcaccgc tgcacttaac atcccttccc ctacaaaccc
     5821 ggcatgggat ggttcatgtc cgcttcctcc accagaaacc aaaccaactt gttccgcttt
     5881 ctgattcttg gctacaacac gggaagattc tacacgatga acaagttctg gatagctttt
     5941 tactaatcct tccagcattt cttcgacaat attgcttgga tcattcatga tttttttcaa
     6001 tttattccca tcctttcttt ccggcatttt gtgcttctgt taagatttgc tgagtatcat
     6061 ctgttatacc ggcagtgatg gctgctgcaa aagctccttc cactaaaggc gcatccacca
     6121 gatgataacg ttgctgctgc tcttcttcca acatgtcttt tgccagttcc gagttcaata
     6181 ccgcgctacc gatatccgca aaaacaaaat aactagctgc atcgcttgat tcattgactg
     6241 cttctacgat tttcatcggg tctgatccga tctcgccatt tattccgccg agagaaaaaa
     6301 tttgcacctc ttcggatttt tgcatctgct caatcatttc tttaatacca tctgttactt
     6361 tttgactatg agaaataagc aaaatacttt ttttcacaag aatcttcctt tcttttacat
     6421 agttgctaac gattggtttc gctttcatta atgaacaatt caagtatata ataaatttct
     6481 aattttggga aaatatgtgt agtgcttctt gaagtttgtt atactttaat taaaagagag
     6541 gagtgatcga atgattacaa caacaacgaa cagtgtagag ggaaaagccg tagtagctta
     6601 taaagggatc gtttttggtg aggtcattac tgggatccat gcatttaaag acttggaggc
     6661 ggggttgcga aatatctttg gcggccgctc aaaaagctat gaaaacgaac taatgggtgc
     6721 aagagaagaa gcacttgccg aaatggccga acgtgcaaaa gcattaggag ctgatgcgat
     6781 cattggcgta aaaatggact atgaagtatt aggcagcgac aatggcatgc tgatggtcac
     6841 atgtagtgga acagctgtct ccacgagata agtaggggac atctgtttta ataactaaag
     6901 agagatggga catgtagtgg cactcaagaa ttagattttt cagtctaact tcgaggtact
     6961 ctaccttccc atcctctctt ttttatttct ttttgcggat gattccgtaa gctgctgcga
     7021 ctaacacacc cgcccctgct aaggaataac tgacagaagc tgttttttca tttgtttttg
     7081 gcaaagtctg acttgttgat ttggatggct ctgtcgtttg ctgaactgtt tctgtcgtgc
     7141 ttccagaggt cactgatttt tctcctgccg aatgcaaaat atcttttatt cgttggaaac
     7201 tagctgctaa gtcagctttt tttgcttcaa aatcattagc aactgtttgt gcttcgctga
     7261 tctttccgtg gattgcttga ctggccgctg tgatatccgc ttgagtttga cttagctttc
     7321 cagaagctgt ttgagcaacc tgatttaatt gctgattgaa ttcttttgct tgttggatct
     7381 tcccgtcaag atctgtttgg acagcttccg cttttgcttt cgcttcggca agctttttct
     7441 caagttgtgc acggtcattt cccactttat ttttaagatc agctaaatct tgatcgattg
     7501 ctgcttggtc ttctttcaat ttgttttttg cagcttctaa tgcttcttcg agttcttttc
     7561 ttttattttc caagtcttgc aaatgatcga tttggtcaga agaggtagaa ctatttattg
     7621 atgtatcttc tgaagatgaa atcgtttctc ctgaattatc tgtcggtaca ttggaagtca
     7681 tttctgtttc cgaggcaccg ttttcagcac cagaagagct tatggttgtt gaaggttctg
     7741 tttgagcagg ttcagaagtt tccgcaaaag ctgtctgtcc caataagaaa atactggaaa
     7801 cagcaattgg ccagattaac ggcttcattc tttttttcat cacttttccc atccttacta
     7861 ctttttaact aatttcttat tataagcaaa aatgtttgga agaaccataa acacaacagg
     7921 ctgtttgtct aaaaaacggt gcatctttta tttttttgta cgttccgggc ttttccatgt
     7981 cccgcttcca taacctaaaa tcgttttgac tgctggaatg aaagtcgtca cgatcgttat
     8041 cgcgttcact atccagaaca aaagcatata tagtggtgcg aaaagaaggt atttcaattt
     8101 tcttcgtcga tcatctacaa tcagcgaagc tagcaactgg aaaaatcctg cgaccatttc
     8161 aaagcagatg aacacaaaac tgaatgtaaa agtaatataa atctgttcgt agtttccttg
     8221 atatacataa tgcccgatta atccagcaaa aagaactacg gaaatacaaa agaatattga
     8281 ccagacaata ctcaatgttt gatcaatgaa gatgatcgtt cttccaatat gtttaaaagg
     8341 atggagcatc acttttttga aattcgtcag ccagacttct gttccacctt ttgcccaacg
     8401 ttttctgtgt cgatatagca tcttcaacgt cacaggtact tccatgaaaa agataatctc
     8461 aggagcaaaa acagataacc agtcatttaa ctgatgatcc caagcaatac tgatgtcttc
     8521 tgtcgctcga tcttgtctga atccgcccac atcgatcaag gcttcttttc gatacatcgt
     8581 atttgctcca ctgtacgcat aaagaccacc gaaaacagct gactgtgttc gtttaatgat
     8641 gcccacgata cttgagaatt caaccgtttg agattttgca atcaatttgg tacgattttg
     8701 gacatccata tttgcagtga ccgcagcaat atgtcttgca ccagggcgaa tgaaataatt
     8761 cacataccta atcagtgcat ctggctccgg taccgtgtct gcatcattgc ttaaaatcaa
     8821 tttccctttt gcaaaggcaa gtccgatatt aaacgcgtgg gcttttcctt tatttttttc
     8881 gatccgaacc acacgaagat tagcgtattt tttcatcagt ctagctaata tttctggtgt
     8941 ttggtccgtt gaaccatcgt ctgtcactaa tacctcatag ttgtgataat tcaatttcgt
     9001 catcaaatac tcgattgtat attcgatcac aatttcttca ttatgggctg gcaccatgat
     9061 cgtgatgaac ggttcgacac tcaagggaac atctacccat tctttttgct tatgtttgta
     9121 taaaaacaca taacaccaga caccaataaa ccatacaaat ccgcctaaaa tcggataaga
     9181 aacgagaaaa aaatacataa tagacaaaca agtatctaag aataaattac ctgtatgggt
     9241 gattgagatc atttcagttc caccccattt tctttataca aatctgcaat gaatgtcgta
     9301 tctagatttt tctcttcgga gacagaataa aaacggaccg tttctctctc ttcttttgtt
     9361 ccaaaacgtt ccgtatacac ttcttcaagt aattcttgtc tttttttcaa tttttcatct
     9421 tcatattcaa aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat
     9481 acaaatgagg taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac
     9541 agcattttct tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc
     9601 gttaatggga aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc
     9661 actaacgttt gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc
     9721 aaaattgatg caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg
     9781 ttacgacgta ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt
     9841 ttcctaagaa aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt
     9901 atcagttgtt ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg
     9961 cttttcattc ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca
    10021 tcgttttcaa atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa
    10081 ggaatgattt ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt
    10141 tgggtttcct cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa
    10201 atctcagcta gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct
    10261 tttccttgcg gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg
    10321 aaaagctctg tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc
    10381 ggcagatggt agtcgtcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag
    10441 attgcctgat tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat
    10501 agacctaatt gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct
    10561 ggtacattta ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca
    10621 acagtacgtg tgggttgcca agcataagct gagccatatc gtttttcata aattctttca
    10681 aacaactgat gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat
    10741 tttttttgta aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc
    10801 aatttaccga ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca
    10861 gcaggacttt ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc
    10921 gttgcttgaa gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg
    10981 atcgtttttt tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga
    11041 tgatctaaaa acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa
    11101 cgttctgtct gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct
    11161 tctatttcct ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt
    11221 ctttcaccta atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat
    11281 agaatcagca agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata
    11341 cgctgcatga tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga
    11401 cttttcacat cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc
    11461 atttcattgg tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca
    11521 gacgtatcaa aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta
    11581 ataaccgtat cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg
    11641 taaaaaagtt cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct
    11701 gtgaaatttg ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca
    11761 acgacttttc caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct
    11821 gcgtttttct gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa
    11881 aaagtatcca ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct
    11941 ttcaacgact ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt
    12001 ttatcaatta gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg
    12061 ttgaccatac acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt
    12121 aagagtcgaa tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg
    12181 tcggacagtt tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg
    12241 cttcaagatt ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc
    12301 ttgttggctg tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc
    12361 ttccttgaat ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc
    12421 ttttgctagt tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat
    12481 gttcattgct gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc
    12541 atacgtcaat tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc
    12601 taaaatatcg atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc
    12661 gccagtctct ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct
    12721 tgcatgatct tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt
    12781 tctttcactt aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc
    12841 aataccgcct tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg
    12901 acgattggct ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac
    12961 aacgatagca tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac
    13021 taggataccc ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg
    13081 acgaacacca atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg
    13141 caatcgtttc ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc
    13201 ttgatgagac aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat
    13261 atgtggtgct tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat
    13321 ttccaatccg atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc
    13381 atgcatcact ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt
    13441 tatccctgga atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta
    13501 ctgttcaatg tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat
    13561 ggctgtcact tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg
    13621 cgtttggaca agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc
    13681 gcaatttgca aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat
    13741 taatcggcga agccgtgtag cagcagacat tccacctgct acacctccga caataacgat
    13801 tttcatctta tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat
    13861 tcactacatc ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg
    13921 attgacaaat cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta
    13981 atggaacatt tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat
    14041 caagtaacgc tggattttcc aaaagctttt cctttaattc tgggactgtt atcgatttta
    14101 ttgattgaaa tagcaaattc attttctgca cctcatttac atttttataa atacctcatg
    14161 gggtatatta aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca
    14221 ctacttttta tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag
    14281 gagaatgtgt tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg
    14341 gacaaattcg tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca
    14401 ctcaattaag cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata
    14461 atctgaaaaa ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag
    14521 aacaagcgat caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc
    14581 catattgatg aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa
    14641 gcttctccag aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta
    14701 gactctgcca atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat
    14761 gctatgcgtg ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca
    14821 gcaggtgtcc ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag
    14881 tcagtagagg ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga
    14941 agctacttct ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat
    15001 aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca
    15061 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag
    15121 tattagagaa aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc
    15181 gaaggtcagc tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg
    15241 agtattaaga aaaattttgt gaaaatggat ttctacattt ttgcataaat ttggcttaat
    15301 accgagaaac tggcttttga ataccgttta aggaagaggt tgtaaaatca gcggtttgac
    15361 ctgagtatta gagaaacaaa cggaaaaata gctgctcata ttttgccgtt ttttgagcta
    15421 ataccgaagg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac
    15481 tccgaatcac aagaaataat ttcaaaaaca acttctcctg tttttggttc ggttggctta
    15541 ttatcgagaa gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac
    15601 accccttttt tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga
    15661 ttgtaaaaga ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc
    15721 tgggaaaagc agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata
    15781 ccacttcgct ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat
    15841 aaagccttct tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc
    15901 catagacaaa atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc
    15961 taagtctgtt acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc
    16021 tgagacaaca atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata
    16081 aatcggcagc aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt
    16141 acgccagtct gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt
    16201 attttagctt acacggggaa taaaaccaat gatttcactg tttttatttt tctttacaga
    16261 cactgatatt atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt
    16321 tcttcctgtc gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc
    16381 tctaggtcac gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa
    16441 cctgtcgggt acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg
    16501 attatttttt tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac
    16561 gcttcttttt ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctgacgttct
    16621 tttttattct ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc
    16681 cgataggctt tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc
    16741 attttatgag caatccacaa agcttggctt tctcctgttt cttccaaccc gcaactgata
    16801 tttaggacga agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc
    16861 tccggcgaat ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc
    16921 atacatagat tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact
    16981 gccaatctca tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat
    17041 gttttgcata tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac
    17101 atcgacgaaa atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat
    17161 accgaacatc gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc
    17221 ggcatttgct ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg
    17281 taccgcatgc tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt
    17341 attgattcta ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat
    17401 atctaacacc gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc
    17461 accagtcaat gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg
    17521 atagtgttca ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt
    17581 tgattcatcg ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt
    17641 atagtattcg cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg
    17701 acgcaagaat ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg
    17761 tactttacgc aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc
    17821 aatcaactct tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg
    17881 tgtctggtat tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc
    17941 cgcatattcg atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag
    18001 tgcatcgatc ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg
    18061 tgcttctctt gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat
    18121 atcgctgatt cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat
    18181 ccgatcaaat tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga
    18241 gatacctgtc ccaaaataaa taagggccta gagataaata aaaggataga acacagctta
    18301 aaaaagtcat gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt
    18361 ttcataagca caccaaataa actttgttta ttggctattc gacttactga ttagatacca
    18421 gctacactca caaaacatac cccattcttc taggtcttat ttattttcca cttaactata
    18481 gcaagacaca ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa
    18541 tagacaccta ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat
    18601 gcccaatctg aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca
    18661 aagtcccgag cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct
    18721 aagatcaatc cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa
    18781 gctagcaacc caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga
    18841 taattccgta tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt
    18901 gcattccaat gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa
    18961 acttgcgcaa tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata
    19021 gctagagaga ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc
    19081 attgttacag cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta
    19141 cttttgttta aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc
    19201 ccaataaact cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt
    19261 tcccgcagat ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc
    19321 tcttctgtcc attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca
    19381 agctgatctc gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc
    19441 aaaaccattt cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca
    19501 ggataaggtg ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa
    19561 tcagcgatat aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat
    19621 tctgtcactg tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt
    19681 ttagagtcga tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat
    19741 cgcaaagctc cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc
    19801 aaaccatctg gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca
    19861 tcacggctgt tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt
    19921 tccttatatt cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca
    19981 ttatctccac cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc
    20041 cagcttgctt cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca
    20101 tccaatagat aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa
    20161 tccgtatctg tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc
    20221 ccatcttgct ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct
    20281 acaactaaat ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc
    20341 aagtttcgta ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt
    20401 tcgatcactt catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca
    20461 tataaaaagc caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt
    20521 ttgatattat caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg
    20581 ccttttagcc cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc
    20641 gtataattct catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct
    20701 actttgaccg agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc
    20761 attggatctg gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact
    20821 gtgtcagcta ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt
    20881 ttggtcgagc gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg
    20941 attcctgatg ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc
    21001 aacagatcca attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa
    21061 aacatatgga tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc
    21121 gttgtaaagt ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc
    21181 ataaaattcg cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa
    21241 tgctgcccct agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat
    21301 atctgtttgg aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac
    21361 aggaatcttg attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc
    21421 aatcgactgt aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa
    21481 catagcccct cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac
    21541 aacataaacc tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt
    21601 ttgcaacatt tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact
    21661 tccttctaaa gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt
    21721 atttttggat aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata
    21781 agtattcttg accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc
    21841 tcctgccatc cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc
    21901 ataaacttct gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc
    21961 taaaatctcc tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt
    22021 cgagtaatca gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat
    22081 cgttccaaac atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag
    22141 aatccagcgt atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg
    22201 gaaaaattct tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga
    22261 ttgccaaaca actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt
    22321 ttcacgctgg ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat
    22381 gaacgctccg gcaataactg actgtacaga attccagatt tcatttgcat catgttccac
    22441 ccaactttct tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc
    22501 tttcttgtca aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata
    22561 cgttgattct gtcattgtga ttccctccat catatgcaaa cgctttcttt aactactctt
    22621 atcatactac tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg
    22681 acagtttttt attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat
    22741 tcttcttttc aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca
    22801 ctgttttttg tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata
    22861 acttccaatc tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc
    22921 aatacgataa atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata
    22981 aaaatcgatc atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc
    23041 ttcagctctt ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg
    23101 tgttaaggaa tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac
    23161 ttcgatcttt ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac
    23221 tgccttttct tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg
    23281 aatgtaagta tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata
    23341 aaatatttct tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc
    23401 gtatacattc agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc
    23461 ttgataaagc cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact
    23521 tttttcattc aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc
    23581 tgatgccgta aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc
    23641 tggtgtattt tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata
    23701 ataaaaatat cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc
    23761 aaattcctct aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat
    23821 attaaacgct gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag
    23881 catcttaaac ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc
    23941 ttcgtccaac acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat
    24001 cattcggcca atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg
    24061 gacatgctct ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa
    24121 aatcaggatc tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat
    24181 tcgccacctt tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat
    24241 gttcttctaa ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa
    24301 ggtggaacat ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa
    24361 atagaaattt gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc
    24421 ctgtttcaat tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta
    24481 agaaatagcc aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg
    24541 agccactaat tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa
    24601 catacatttt ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa
    24661 cgtctttttg agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg
    24721 ttaattgttc ttcccacatc tgattgtcgg gacgagaatg attagccagc aaaacaagat
    24781 catagttctc ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca
    24841 caacttgttc ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt
    24901 ctgtcatttc aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga
    24961 tttgtttttt tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa
    25021 taattgcaat tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct
    25081 ttttaactgg gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac
    25141 cagtcgctag aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt
    25201 tttctttcaa tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca
    25261 gttcttcttt cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca
    25321 aattgaaccc gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac
    25381 ttgttggcat caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga
    25441 caattacaat gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca
    25501 atcagaaaac acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag
    25561 aatcaaccct ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac
    25621 ctaattgaag agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa
    25681 aaaactcgcc ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag
    25741 ttttagcgtg tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat
    25801 gcttcaacaa ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc
    25861 cgcccatcaa tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt
    25921 tctttcaaag catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct
    25981 tcttcattac tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct
    26041 gtaactggca acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga
    26101 tagatttgtg aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg
    26161 tggccaccag cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag
    26221 gtatttccca aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca
    26281 tccatattca aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa
    26341 tctgcatcga tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac
    26401 aaacctgtag gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact
    26461 ttcattttcc catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat
    26521 tatataatgg gcttggcaaa taaagctctt tatatgcttt ttcaaaaggt ttcgttacaa
    26581 attcatcctc acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct
    26641 gtccaattgc tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga
    26701 tcccaccgac accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa
    26761 tcgatttatc gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat
    26821 gcagataatc tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac
    26881 ggatacctgg tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt
    26941 tagcatattg ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac
    27001 gattttccaa gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt
    27061 gttggatcaa atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg
    27121 ctctacgtgt ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg
    27181 ctcgtggtgt ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt
    27241 ctccagataa agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt
    27301 ttgcgccttc tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat
    27361 gagcgatgct gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag
    27421 taataacagc accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga
    27481 tcactccatt gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca
    27541 attgaactcc tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca
    27601 taattcgctc tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta
    27661 catgcaacta atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc
    27721 attgtctggg ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga
    27781 agttcttccc agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa
    27841 actaatgaca agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca
    27901 acaaaacgag caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt
    27961 attccatctt tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc
    28021 cttctaaacg cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag
    28081 aaaagacatt catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg
    28141 tacatatcat agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct
    28201 ctgtctagat ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca
    28261 aaactccttc tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat
    28321 gaagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
    28381 aaaactggct tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag
    28441 taataagaac agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc
    28501 gatggtcagc tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga
    28561 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga
    28621 tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt
    28681 gtcacagtcc cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct
    28741 gtaccaatca aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg
    28801 atcaacatag atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc
    28861 aatgcagccc cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat
    28921 ctgctcaata tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc
    28981 ttctactttg tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct
    29041 atcatataat tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc
    29101 acttgatgga agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc
    29161 ccaattttct ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc
    29221 aacagggtag gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt
    29281 ttcttcaaac attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa
    29341 gaacaaagaa gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata
    29401 cggaacaaga agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg
    29461 acaagctttt gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc
    29521 ttgttccaaa tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt
    29581 tacacagttg atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa
    29641 tactgtttca gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata
    29701 ttctgtaacc aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc
    29761 atattttaga cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt
    29821 ttcttcatca ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa
    29881 acccatttca gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg
    29941 tcttttagga taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt
    30001 catcatatgc cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac
    30061 atcagacaca gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc
    30121 tgtcgctccg ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt
    30181 gctagttttc ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta
    30241 ctatttgtaa caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa
    30301 tctacttcca tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga
    30361 aagtatttgc catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct
    30421 tcttcactga ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt
    30481 acagaactgt agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca
    30541 ctagcaaatg ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta
    30601 cttaacacta tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa
    30661 gttagcatac aggaactgat catcgctaag aaataactag caactagcca aacaaatcct
    30721 tcaccaatat aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct
    30781 cctgcggcac cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt
    30841 cgtttatatt taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg
    30901 ataaatgcag ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca
    30961 agtactgctc ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga
    31021 ccgtagaaag aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc
    31081 actaacactt gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa
    31141 acagctaaat atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact
    31201 aataaaataa ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata
    31261 ctttcgtgca agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa
    31321 attgctggaa taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc
    31381 aaggttatgc tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata
    31441 accatagcga tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata
    31501 ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca
    31561 gtagttaata aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct
    31621 gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa
    31681 atgccttttt tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt
    31741 ttggataata cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct
    31801 ccagcttcga ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc
    31861 ttattatctt ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt
    31921 tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact
    31981 tcccccttgt taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa
    32041 aacggtttct ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt
    32101 gtatggttta cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt
    32161 ctcttatagt caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt
    32221 ggtatctcaa gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac
    32281 aacaaagtct cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc
    32341 tgaatggaat ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca
    32401 agcagttttg ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa
    32461 atctcccaag attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca
    32521 aaaataagct cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga
    32581 tttttgaaaa gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt
    32641 tgaatctgtt tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc
    32701 tccccttgtt ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt
    32761 tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt
    32821 tctaaaggat aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca
    32881 tccatcctcg ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat
    32941 aacgaaacag gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc
    33001 tctatttctt tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt
    33061 tcaattgctt gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt
    33121 gtgagggtct ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct
    33181 tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa
    33241 acaatataat tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata
    33301 ttggaaaaaa ttgtactgat ttgttgagga aaccttgcgt gttggatcac cgtttctgtt
    33361 ccaacaagag ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt
    33421 gtcgacgaaa cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt
    33481 atttgtaatg atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg
    33541 acataccctt tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg
    33601 gaatagattt tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt
    33661 gattctaaaa aataacctga acgtttattt gactggataa tgattttctt ctcgtagata
    33721 gcaagcatat ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct
    33781 aattcttcgc ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt
    33841 cgtttattta agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct
    33901 ctcaaatgac aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc
    33961 aaggttttta ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa
    34021 tctcctaaag cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt
    34081 cctcatttcc cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat
    34141 gatttggcaa ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc
    34201 acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc
    34261 attttcaact atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg
    34321 aaaccctaga ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg
    34381 attatcgaaa ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac
    34441 agcgctacga taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc
    34501 gtctccatct aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc
    34561 tggataatgc cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag
    34621 tgaaccaaat acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct
    34681 acacataata cccatcttag catttggatc gattttcttt aataattgag tagcacgggc
    34741 gttcgctaga aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat
    34801 gggttcaagt ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg
    34861 aaaagcatta ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc
    34921 aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc
    34981 atactctcct accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat
    35041 atcatatcga tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg
    35101 cttctgttcg tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt
    35161 taaacctaac tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc
    35221 ttcatgagaa agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc
    35281 attccactga atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg
    35341 taatttgccg tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca
    35401 taaaaaattt tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt
    35461 tcttacttga agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag
    35521 agaagaaaaa aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa
    35581 gacgatcccc gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca
    35641 acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat
    35701 aatttgacac aataaccctt aaagcttcac gtggaatagt gaacgtctgc ttttcactgg
    35761 acatattaca aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat
    35821 gctcaggcgt cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac
    35881 gcaaatcgat caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt
    35941 ctgcattgat ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc
    36001 cagcatgttc acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcct
    36061 tgattgattc caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat
    36121 tgattgtctc aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta
    36181 actcttctcc ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca
    36241 tcttggcact ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa
    36301 gatcgtgatt attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt
    36361 tggataatac ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc
    36421 cttcttgctg atctaatcct atgtgttcga attggaagac catggataat tcatttcttt
    36481 taggattgga ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca
    36541 tcagattttt gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag
    36601 gtccattact tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac
    36661 gaaagccccc aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa
    36721 cctgtttatt tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt
    36781 gccccgtttt ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag
    36841 gctcttttcc attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat
    36901 ttttacgggc ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca
    36961 tgatgatacg gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg
    37021 taccgaattc tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg
    37081 gagactcgta tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca
    37141 acctggaaat aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc
    37201 ttggatagat ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc
    37261 tataaatgga tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat
    37321 tgtaacgctt tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac
    37381 agctgaggaa attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct
    37441 gatttcacaa cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa
    37501 aacggcaaaa tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc
    37561 tgatttcaca acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga
    37621 acagaccata catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg
    37681 gatagaagat aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga
    37741 tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag
    37801 attgtgccat caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa
    37861 ctgatagtaa tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg
    37921 tgatacctga acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag
    37981 gagaagtacc aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc
    38041 ctacacgagg catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag
    38101 ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa
    38161 gagaattgat gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg
    38221 ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg
    38281 tagctgccat cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta
    38341 cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta
    38401 atgtagattc attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata
    38461 gaccgatacc gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt
    38521 cccgcagctt gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta
    38581 atgctgtttc ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga
    38641 tcccaagagc aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc
    38701 cgatcgcact ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac
    38761 ctaatacagt tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg
    38821 ccagtacctc acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt
    38881 tttccttcat cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc
    38941 gatttattaa taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa
    39001 aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt
    39061 tcatgaacat tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat
    39121 tctagtctca aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg
    39181 atgaattctt ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc
    39241 atttttcccc agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc
    39301 tcactatgtt ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact
    39361 gtttttcctc ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc
    39421 ttcagggtca gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc
    39481 ttccatgttc tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg
    39541 tcctaatacc tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa
    39601 ttcttttgag atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga
    39661 aatttctgca ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata
    39721 aagcatcaca cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc
    39781 ctgtctataa actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat
    39841 tcaagaaaaa taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa
    39901 aaaaaaacgg caatgaatgc cgctcttctt atttcgattc ttttataatt ctgcttctga
    39961 ttcatctttg gttagtgtgt gtctaacact agttacgcca aatgtttgat cgattttttc
    40021 cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt tcagcattga tggattcaat
    40081 gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt cacgccagtt
    40141 gagacagcga cagatcgctc cttgttcatc atagtagatg atttcttccg gaagtgccgg
    40201 agcatcggaa tctgcaccta aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg
    40261 cagatttcct gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa aagacaagga
    40321 aatactatta tagatatcta caagcggatt gattgggaaa aattcccgtt cttgatttgc
    40381 acgtttcagt aaagcttcaa tagaagaacg tgcacctttt ttagtcttga atgtggtaaa
    40441 tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat gtttcttctg taaggtaatt
    40501 tcttgcttct tttgctgcct cattcaataa ttgatgatac gtttcttcac tttcttttgt
    40561 tacgtggtta tcgataccat ttacggataa gatttcgatg gttgcttcag gaaatacttc
    40621 ccagaaattg gtatccacta ttactttttt catctgttca cacctcttga ttagaataat
    40681 tgcttgatga atagatagtc tgtttgggga tcatctccca tataaaaaac atgttcgctg
    40741 tatttgataa atcctttttt acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca
    40801 ccgagccaaa tggctgtttt ttgcttttct tttgcttttt caatagctgt atctagtaaa
    40861 aatgtaccga taccttgatg tttaaatttt tttcgtacat agattcgctc gatttctaaa
    40921 aaatgatcag ccatttcctc agattgactc gttcctgtat ttagtttcag gtaacccgca
    40981 ggctcttgat cgatactcac taggaaaaaa tcggtttctt tatcttttag ctcttctgta
    41041 agtttttcta gtgcataagc ttgtgtcaag taatcagcta aatgctccgg tgaattatct
    41101 tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa
    41161 ggcgtcattt ttcgaatttc gtatttattc acggcttctt cctttcaata gatcctttta
    41221 ttgccttttt tgacaaaatt ccaatcttcg ctaacattct tgcatacttt atttaaaaga
    41281 tattccaatt gttttgcttc ttcgtcagaa agaccattta atgcgactgc attggagtag
    41341 ttgttttctc gaatgatgaa aggatagatt tctgctcctt ttttagtcgg atagagtttc
    41401 ttgatttttt tattttcttt atcttccaaa cgttcgatca tcccattgga ttctaatttc
    41461 ttgattgctc gggcagtcgt tgtacgatct actttgatca tttctgctag tttttcttgg
    41521 atgattccag gattttcgca aatacgtact aaataaagat actgtcctct tgttaaatcg
    41581 acttctttga attcgatatt gctaatagaa tctagcgctc gggcaatcat tccgatgtct
    41641 cgaaggattt cagccatttc atcccacctt tattttgttt gtaagactag ttcacctctt
    41701 tttgttgcaa atgcaactat tttcaataaa aaaatcaagt tcttttttaa taactagtaa
    41761 agtatattct tttcttcttt tacgaaaaag ctcaatcaca atagatctgg tttttgtttt
    41821 ctaaaataag taactgtttc ttcgttgttc gcttttctga tagctttggc aactcttcta
    41881 tactaaaaaa tcccatgttg gaagtttcat tattctcaat aaacttggcc tcttcaccgt
    41941 ggatcgcaca agcaaaaaca agtttataat actgaaaaag ctgtggaata tctttttgtt
    42001 tattcgtatc aaaaactgct cgcaactgag tagtttctac tgttaatcct gtttcttcat
    42061 agacttcttt acgcacattt tcttcaggag acaatcctat ttcagcaaat ccaccaggaa
    42121 gtgaccattc tttcgtccgt ttatcttcga ttagtaggac cttgccatct tttttgatat
    42181 aggcacgaac atctactttt ggtgttggat gtccttctcc aatcgttgtg agcgctttta
    42241 tttcttcgaa agtttctttt cctatttcac tgatcagttc caatgaaatc gtccggatct
    42301 cctcaaaacg ctctttatca aatacatctt ttccataata tagaccagct tgagaaagtg
    42361 cgagtagtct tttgtattga gccaacttct tgttcattct cttcccttct tttttagcaa
    42421 atgtatgact atgtaatcaa gtatagaaga ctggtttaat tacggcaaat aaatataatg
    42481 aaatctgcaa taaataaaag aatgttaaaa aactcgagaa caaaagacat tcgacttctc
    42541 tggggctgtg aataaatggc gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac
    42601 gaaaaatatg acgagctatt cttgtctggc tgttcttatt aattgaggca ggacagcttt
    42661 ttcacaacct ccccactaaa cggtgttctc ctaccaactc ctcggtcaca agtcacaata
    42721 ttcatcaaac atgagaagct gtttttttga aattgctcct tgtgactcgg agctgaacga
    42781 caggctcacg acctctcggt aaacagcgtt caaaagccaa tttttcgata ttaagccatt
    42841 tctttgttac ttattgtcat tgatttcttt ttctaattta tccaatagtg gagtgtaatc
    42901 cacttgagta tagtctgtta catcaaattc atatagtgtt cctaactgga atttgtcata
    42961 ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta atatgattgg tccctttcgt
    43021 tcgatcttcc aatacgtcac catagtgata atgatccatg atatagctca aatgacgatc
    43081 tgattcacga tcacgtttgt accgacgttg ccataacact tcaaagtctg ctgttaggcg
    43141 aatcgtgatc acctgatatc cgtatctatc tgctaattct tggagtcgtg ctttttgctt
    43201 atcactaaaa ggatactcgg acacaatcac tttctttcct gcttccatat acaaatcaag
    43261 tgctttgtaa tagaaaggat aaactttcgt cctttcttgt tccgcccgtt cttccaaatt
    43321 gttaaacccg atggattctg catagtattc cttgatttca tcgagcgtga tggcatacat
    43381 atctggaaat cgttcccgta gtttattcat aagatatgtt tttcctgtac caggactacc
    43441 agccagaaga atgaaatact ttttcatctc atttatttcc taagttaatt ggactttcca
    43501 agttcataaa cagtccaatc ttatgcttga caatttcttt tactgcttca tttgctggaa
    43561 tcacattatg gcgaagagac ttgttgattt ctgtttcact aaaggtttct ttagctactt
    43621 tcgtccagtt gactaaaagc tccgttccaa cattgaattt gcggatcccg ttattgatca
    43681 attcaggata atcttcttct ttcacacctg ttcctccatg gataactagg gggacatcaa
    43741 ctactgcatc gatttctttc agcaagggaa tgtttacttc tgtttttgat ttgaattgtc
    43801 catgattcgt accgatagca atagctaatg catccacgcc tgtagcttct acaaatttca
    43861 ctgcgtcttc cggtcttgta tataccttgt cattttcagc gacatggatt ccttcttctg
    43921 taccgccgat tgttccaagt tctccttcta cggaaacacc gtgcgcatgg gcatattcta
    43981 ctactgcttt tgtcttcagg atattttctt tgaaaggcag atgggaccca tcatacatga
    44041 cagaagtata tccgctgtcg attgcttctt tgatatcatc aaagtctcgg gcgtggtcta
    44101 agtgcaagac tgcatcaacc atttcttctt ctgcaatagc tgtcattaca ttgactaaca
    44161 catgcatgcc gatatattta gcggtatcga cacttgtttg aataatgatc ggcgaaccca
    44221 tttcttttgc tgcgcctagc atagcaggaa gcatttctag gttatgcgta ttaaaagctc
    44281 caactgtaaa attcaactct tctgctgttt ttgttacttc tttcaatgtt gtatacatct
    44341 tttattgcca ccttatcttt ttatttgttt cctttgatcg ttacttgttt tgcaatttgt
    44401 cgcatctcat ccattttgcc catgtagtaa tcgatccctt gatcatcacc agcacgagca
    44461 gcatctgcac gcttctggtt atagaggctc atcagttttt tatacccatc tccgatcgtc
    44521 tgtttatttt ccaaactctt ttctaaatat tggatggctt gatcgttcac atacaattca
    44581 gaaaatttaa gtccaacttg ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg
    44641 gaaatctctt gtttcaattg tgtaatctct gattgaatct tttcggtcgc ttctggcgat
    44701 agggcttttt cttcttttgc cagaactttt ttcggttctt tttctttttt cttaaaaaat
    44761 ccaaacatca ttttccctac tttcaattaa aatgctttca aaactgggct taagatccag
    44821 gcatataaga gtgctgcggc tactgtatct acatcggttg ctgtagcatt gttgaatccc
    44881 atcgaattaa agatcgttac tagcaaagcg ggcaataatg taataaagaa accatgagca
    44941 ataccgccaa tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc
    45001 cctccggcaa agaaattggt tagcatacca ggtaaaatca tcgctaagcc gaacatgggc
    45061 aaagtaaaca tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca
    45121 tttgggctat aaggaaagaa aactggacaa tctaaggcag ggatcgcatc aggaactaat
    45181 ttcatagcta tccctcgaaa tgccggcacg atttcaccta acagtagacg tacaccagct
    45241 agcaatacat acacacccac tacgaattgg atggcttgta aaaaggcaaa catgatatag
    45301 ttttgatctc ctgataattc agaagcgaat ggttcgcctg caaataaaac agtgatgata
    45361 tatagcggga ccatcacgac catgacagac aaataagtat cttgcagaaa ttcgaaatgt
    45421 gtcggcaact tgatatcttc gatcgacttt ttattttcgc ctttctcacc aaaaagctta
    45481 gcaacaccgg cttcaaataa gtacccgatc gtacaaaaat gacctaaagc aatatcattt
    45541 gatccagtga ttttacgaat gatcggttgc gctactgccg gcatagcaat ggcgaaacaa
    45601 gctccgataa aaccaccgac caagatcaat acgatccctc gcagaccggc aaaatagcca
    45661 aagactgtcg tcatcgttgc catccataaa attgcttgac cagttaaaaa aatgtacttc
    45721 cattttgtga aacgagccaa aataatattg aatacaaaaa tggctaagaa cgtcagtgca
    45781 atatctctac ctaaacccaa atcattcatt gcttgaccat tgatagattc aatagaaggg
    45841 atgatccctt gcatatgaaa tccttcagta aagattttcc cgaaataagt taaacttcca
    45901 acgatgatgc tcgaaccggc actcaacact tgaaatccta ggagtgtctt taacgtgcca
    45961 gagatgactt gtcctgctga tttcttctgc agtagtagac ccaacatagc aatcaatgca
    46021 atggtgatcg atgcctgagt caaaatattt tcgatgataa aattgataac gcccatgttt
    46081 tgatgtgcac cccaaaagtt agactagaaa tctaattaaa aggggtgctt tttttgcgga
    46141 aatatacatt tgtttttaag aaaaaagtag tttcagacta tttaaataac gaaggcggct
    46201 acaaatatct tgcacataaa tatcaaataa atcgtacact ggttagacat tgggtaagga
    46261 tttataacta tcatggttgg gaaggcttgg ttggaggtgg caaaagctac actacaaaat
    46321 ttaaacttga tgttatagaa tatatggaaa caaatggtct ttctatccaa gaaactgcta
    46381 aaaaatttaa tatcggttca aatagaactc taagtaaatg gatagagcaa tatgaagaag
    46441 gcggtgcttc ttcacttgag agccaaaaaa ggggcagaaa aattagtatg aattccaagc
    46501 taaacattcc taaaaaactt aaagatgagt ctcttgaaga agaagttatt cgtttaagag
    46561 cag
//
LOCUS       DAZKCC010000014        45472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000014 DAZKCC010000000
VERSION     DAZKCC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.014"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..247
                     /locus_tag="ACG8VH_000819"
     CDS             <1..247
                     /locus_tag="ACG8VH_000819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109397.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
     gene            447..1637
                     /locus_tag="ACG8VH_000820"
     CDS             447..1637
                     /locus_tag="ACG8VH_000820"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9109398.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1739..2692
                     /locus_tag="ACG8VH_000821"
     CDS             1739..2692
                     /locus_tag="ACG8VH_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9109399.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2761..4110
                     /locus_tag="ACG8VH_000822"
     CDS             2761..4110
                     /locus_tag="ACG8VH_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9109400.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4293..4616)
                     /locus_tag="ACG8VH_000823"
     CDS             complement(4293..4616)
                     /locus_tag="ACG8VH_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109401.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4745..5908
                     /locus_tag="ACG8VH_000824"
     CDS             4745..5908
                     /locus_tag="ACG8VH_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9109402.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            5978..7327
                     /locus_tag="ACG8VH_000825"
     CDS             5978..7327
                     /locus_tag="ACG8VH_000825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9109403.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7372..7803)
                     /locus_tag="ACG8VH_000826"
     CDS             complement(7372..7803)
                     /locus_tag="ACG8VH_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109404.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            8191..10833
                     /gene="alaS"
                     /locus_tag="ACG8VH_000827"
     CDS             8191..10833
                     /gene="alaS"
                     /locus_tag="ACG8VH_000827"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9109405.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            11033..11629
                     /locus_tag="ACG8VH_000828"
     CDS             11033..11629
                     /locus_tag="ACG8VH_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9109406.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11751..12899
                     /locus_tag="ACG8VH_000829"
     CDS             11751..12899
                     /locus_tag="ACG8VH_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9109407.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(12937..13350)
                     /locus_tag="ACG8VH_000830"
     CDS             complement(12937..13350)
                     /locus_tag="ACG8VH_000830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP9109408.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13429..13860
                     /locus_tag="ACG8VH_000831"
     CDS             13429..13860
                     /locus_tag="ACG8VH_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP9109409.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(13959..14192)
                     /locus_tag="ACG8VH_000832"
     CDS             complement(13959..14192)
                     /locus_tag="ACG8VH_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9109410.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14375..16045
                     /locus_tag="ACG8VH_000833"
     CDS             14375..16045
                     /locus_tag="ACG8VH_000833"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP9109411.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16192..16578
                     /locus_tag="ACG8VH_000834"
     CDS             16192..16578
                     /locus_tag="ACG8VH_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109412.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16565..17263
                     /locus_tag="ACG8VH_000835"
     CDS             16565..17263
                     /locus_tag="ACG8VH_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9109413.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            17268..17732
                     /gene="lspA"
                     /locus_tag="ACG8VH_000836"
     CDS             17268..17732
                     /gene="lspA"
                     /locus_tag="ACG8VH_000836"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9109414.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17729..18640
                     /locus_tag="ACG8VH_000837"
     CDS             17729..18640
                     /locus_tag="ACG8VH_000837"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9109415.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18875..19414
                     /gene="pyrR"
                     /locus_tag="ACG8VH_000838"
     CDS             18875..19414
                     /gene="pyrR"
                     /locus_tag="ACG8VH_000838"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP9109416.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19428..20711
                     /locus_tag="ACG8VH_000839"
     CDS             19428..20711
                     /locus_tag="ACG8VH_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP9109417.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20724..21650
                     /locus_tag="ACG8VH_000840"
     CDS             20724..21650
                     /locus_tag="ACG8VH_000840"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP9109418.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21669..22949
                     /locus_tag="ACG8VH_000841"
     CDS             21669..22949
                     /locus_tag="ACG8VH_000841"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9109419.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            22954..24030
                     /locus_tag="ACG8VH_000842"
     CDS             22954..24030
                     /locus_tag="ACG8VH_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP9109420.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            24035..27220
                     /gene="carB"
                     /locus_tag="ACG8VH_000843"
     CDS             24035..27220
                     /gene="carB"
                     /locus_tag="ACG8VH_000843"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9109421.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            27274..28050
                     /locus_tag="ACG8VH_000844"
     CDS             27274..28050
                     /locus_tag="ACG8VH_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP9109422.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            28047..28979
                     /locus_tag="ACG8VH_000845"
     CDS             28047..28979
                     /locus_tag="ACG8VH_000845"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP9109423.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            28976..29692
                     /gene="pyrF"
                     /locus_tag="ACG8VH_000846"
     CDS             28976..29692
                     /gene="pyrF"
                     /locus_tag="ACG8VH_000846"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9109424.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29699..30328
                     /gene="pyrE"
                     /locus_tag="ACG8VH_000847"
     CDS             29699..30328
                     /gene="pyrE"
                     /locus_tag="ACG8VH_000847"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9109425.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30316..30630
                     /locus_tag="ACG8VH_000848"
     CDS             30316..30630
                     /locus_tag="ACG8VH_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109426.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30668..31309
                     /locus_tag="ACG8VH_000849"
     CDS             30668..31309
                     /locus_tag="ACG8VH_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP9109427.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31352..32044
                     /locus_tag="ACG8VH_000850"
     CDS             31352..32044
                     /locus_tag="ACG8VH_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP9109428.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            32041..32943
                     /locus_tag="ACG8VH_000851"
     CDS             32041..32943
                     /locus_tag="ACG8VH_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9109429.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(33101..34807)
                     /gene="efbA"
                     /locus_tag="ACG8VH_000852"
     CDS             complement(33101..34807)
                     /gene="efbA"
                     /locus_tag="ACG8VH_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP9109430.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            34997..35620
                     /locus_tag="ACG8VH_000853"
     CDS             34997..35620
                     /locus_tag="ACG8VH_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109431.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            36070..37119
                     /gene="trpX"
                     /locus_tag="ACG8VH_000854"
     CDS             36070..37119
                     /gene="trpX"
                     /locus_tag="ACG8VH_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9109432.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            37116..37997
                     /locus_tag="ACG8VH_000855"
     CDS             37116..37997
                     /locus_tag="ACG8VH_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109433.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            38000..38770
                     /locus_tag="ACG8VH_000856"
     CDS             38000..38770
                     /locus_tag="ACG8VH_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109434.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            39049..39903
                     /gene="aroE"
                     /locus_tag="ACG8VH_000857"
     CDS             39049..39903
                     /gene="aroE"
                     /locus_tag="ACG8VH_000857"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9109435.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            40081..41244
                     /locus_tag="ACG8VH_000858"
     CDS             40081..41244
                     /locus_tag="ACG8VH_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9109436.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41361..41771
                     /locus_tag="ACG8VH_000859"
     CDS             41361..41771
                     /locus_tag="ACG8VH_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9109437.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            41945..44173
                     /gene="topB"
                     /locus_tag="ACG8VH_000860"
     CDS             41945..44173
                     /gene="topB"
                     /locus_tag="ACG8VH_000860"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9109438.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44344..44628
                     /locus_tag="ACG8VH_000861"
     CDS             44344..44628
                     /locus_tag="ACG8VH_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109439.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            44788..45024
                     /locus_tag="ACG8VH_000862"
     CDS             44788..45024
                     /locus_tag="ACG8VH_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9109440.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            45021..45420
                     /locus_tag="ACG8VH_000863"
                     /pseudo
     CDS             45021..45420
                     /locus_tag="ACG8VH_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT        14916 a         7885 c         9869 g        12802 t
ORIGIN      
        1 tattcggaag aataggactg tgacaagact tttgtcacag tccttttttc gaattcaaca
       61 agtaaaactt ttaagaaata ttcagagagt aaaattaaaa attgctgata aacgaagatg
      121 gaaattttat gtatttttat tggtctatgc aataaataaa ttaaatattc agaatacaat
      181 tgattttaac gaaagggctt ctatcaatgg ttgtgttgta tttatctgcc caggtagagt
      241 aaaatagatc attatctaaa aggtaattgt tcatactata taagtcattt gaaagtaaaa
      301 agatactgta aataaaaaat gtggttaaat aattctcata atcagtgtaa gcgttatcaa
      361 aaatatagca tacttgcttt gtagatgaat gacacgatat atgtttggta tgttctgcaa
      421 gacagatgaa tatggaggtg gagacaatgt cttccaaaga actaacaatg tatttagaga
      481 aagaattgga tcaactgaaa gaaaaggggt tatacaatac gatcgacata ctagaaagtg
      541 aaaatggtgc ttgtatcatc gtagacggaa aaaagatgat caatctagca tccaacaatt
      601 atcttggatt tgccaatcgc gaagaattga aaaaagcatg tattgaagca accgaaactt
      661 atggtgtagg agcaggagca gtccgaacga tcaatggttc gctgaaaatc catcagcaat
      721 tagaagaaaa aatcgcagaa ttcaaaggga cagaagcagc aatcgccttt caatctggat
      781 tcaactgcaa tatgggcgct atctctgcag taatgacaaa agaagatgct atcttatcgg
      841 atgaattaaa ccatgcatct attattgacg gttgccgctt atccggagca aaaatcatcc
      901 gaattaaaca tcaagatatg aaagaccttg agaaaaaagc aaaggaagcg atagaaagta
      961 agaaatataa aaaaatcatg tatatcacag atggtgtttt ctccatggat ggagacattg
     1021 cacgattgcc ggaaattatt ccgattgttg aaaaatatgg actgatcacc tacgtcgatg
     1081 atgcgcacgg ctcaggcgta actggaaaag gtgcaggaac cgtcaagcat tttggattaa
     1141 gcgacaaaat cgatatgcaa atgggcacgt tatcgaaagc tataggtgtt gttgggggtt
     1201 atgtagctgg atcgaaaaca ctaatcgact ggttgaaagc tagaagtcgg ccattcctat
     1261 tttctacttc gctaactcca ggtgcagctg cagctgcatt ggcttcgatt actttgatgc
     1321 aagaacatcc ggaacttgtc gaaaaagttt gggaaaatgc taattatttt aaagaagaat
     1381 tgaaaaaagt aggctataac atcggtatgt ctgaaacacc tattactcca gtcattttgg
     1441 gagacgaaaa agtgactcag actttctcta agaagctgat cgaacatggg atttatgcta
     1501 aaccaatcgt ttatccaact gtgcctcttg ggacaggcag gatcagaaat atgccgactg
     1561 cggaacatac gagggaaatg cttgatgaag cagtagcagt ttatcaaaaa ataggaaaag
     1621 aaatggaaat tatttaacta aatttagttt tttcgtctat ttaaaattag agaacatgtt
     1681 cacgcatata ttgttaatca tctgaagata gtagtaaaaa agatcaggga ggatcatcat
     1741 gaaaaaagta ttggtgacag gatgtttagg acaaattggt tcagagttga tcacacggct
     1801 acgtcaagaa ctaggcagtg aaaatgtcat tgcgacagat atacgcatgc ctgaagtcat
     1861 tgagaaaaac gatatctttg agatcctaga tgtaatggat tacgaaaaaa tgcgcagcat
     1921 agcagaacaa tatcaagtgg acacattgat tcatttggct gctcttttgt cagctgttgc
     1981 agaagcaaaa ccacaattag cctggaattt gaacgtgact gggctggtca atgcattgga
     2041 agtcgctaga gaattccagc tgaaattttt tacgccaagt tcaatcggcg cgtttggtcc
     2101 aaatactcca aaagatcata ccccacaaga tactcttcag cgtcctacaa ccatgtatgg
     2161 ggtaacgaag gtagctggag aactactatg tgattattat tatgtgaaat atggcgtaga
     2221 tactcgtggt gtccgtttcc ctggattgat ttcttatgca accttgccag gaggcggtac
     2281 gacagactac gcagtcgata tttattattc agcaatcaaa gaaggaaagt acactagttt
     2341 cattgatcaa ggtacatcaa tggatatgat gtatatgcca gatgcgatcg atgccattat
     2401 aaaactcatg aatgcagatc ctgatcgatt ggttcatcga aatgccttca atatatcggc
     2461 gatgtctttt gacccggaag agatcaaagc atctattcaa aaaatcatac cagattttga
     2521 aatggattat gatgtggatc ctgtacgtca gtcaatagca gaatcttggc caaattcact
     2581 agatgtttct tgtgcgcaag aggaatgggg cttttcgccg aaatacgact tagatgtgat
     2641 gacaaaggat atgttggaaa aactgaaaga aaaattggta gagacggtat aaaacaagaa
     2701 ccacatccaa aaagtttttt atacttttgg gattggctat ggtatgaaag gggacaaggg
     2761 atggaagaat atcaagccac gccagtaaaa gaagtaaaaa tagaaaatga attaaaacgt
     2821 acgatgggat tttttactgc tttatcgact gttatgggca cagtgatcgg tgcaggggta
     2881 ttttttaaag ccgcaagcgt agcagaagtt acaggatccg ccagtttgca tatgttttct
     2941 tggtttctcg gtggtatgat ttctgtctgt gcaggactaa caggagctga actggcagct
     3001 gctattccag aaacaggagg gatgattaaa tatatcgaac ggatttacgg aaatacagca
     3061 gcttttttat tgggctgggc gcaagtcgtg atttattttc cagcaaatgt agcagccttg
     3121 tcgatcattt tcggtacaca gttcgtcaat ctatttggtc tttcccagtc aatgattgtt
     3181 cccgtagctg ttacagctgc tgtatcgatc ctgttgatca attttttagg ttctaaagct
     3241 ggaggggctt ttcagtcgat tacgcttgtc tgcaagctta ttcctttgtt tgtgatcgtc
     3301 attttcggtt tgttccgaca agaaggagta gacttccaac tgtttccgat ccaagcagga
     3361 gaaaatcttt catttttctc agccttggga gcgggcttgc tagcaacgat gttcgcatac
     3421 gacggttgga tacacgtagg aaatatttcg ggagaattga aaaagccggc gaaagatttg
     3481 ccaaaagcaa tttctttagg aatcatcgga atcatgatcg tttatttatt agtaaatgcg
     3541 gtgttcttaa gaaccgcatc tatcgacgga gtcgcaggaa acagcaatgc ggcaagtgac
     3601 gttgcaaaaa tgatttttgg cggcttcggt ggaagactgg tcacagttgg tatcttgatt
     3661 tctgtttatg gaaccatcaa tggctatacc cttactggaa tgcgtctccc ttatgttatg
     3721 gcaaaagaaa acaacctgcc ttttagcaag ctctttgcta aacttcatga taaaactaaa
     3781 gttcctgtag ccgcaggtat cttagagcta gtcatcgcta ttggcatgat gatgatcggt
     3841 ggttttgata cactaacgga tatgctgatc tttgtcattt ggatttttta tacgatggtc
     3901 tttgttggtg tcattcttct tcgtaaaaaa gaacctgatt tgtttcgacc atacaaagtt
     3961 ccaatgtatc cgtttattcc actagtagca atcattggag ggacttttat cgtatcaagc
     4021 acgcttatca ctcagacctt tttagcttcg atggggatag tattgacact tgctggtatc
     4081 ccgatttatc tctatttaaa acgtagatag ctaaaacatt cgaattttat tagaactgca
     4141 cttctctgcc aaacaaaata ttttatttaa atgaggagta cgtggaagaa atgtcaaaag
     4201 tactcgttaa ttttgatcac gtaaatgtcg ttttgtctac gatgaaatag caaagtaaaa
     4261 gacagaaatc gggctcaacc gatttctgtc ttttactttg atttgcgtaa ctgaactatt
     4321 cggcgtacgc cgcgataaat atttaataaa accaagcaac cagaaatgaa aatgggaagc
     4381 caaatcagat agacagggta gccaagcact ttagacaaag catgtggagt cattttatcc
     4441 atttcaagaa gtgcttgctg ccaagtctga agattgaaga ctagcatgaa acccgaaata
     4501 atcaggagaa taaatcctaa ataatgaatt gtagttcttt ttcttacata agcgatcgta
     4561 gcataaagga acagtaaaaa aataggtacg ccaaaaacaa agtacatatt cagcatctcg
     4621 acaccccctt tgtgtttatc ttactttact ttgaaagaaa tgagaaataa tagcctcgtt
     4681 acttgcttga aacagcaaag gactgctaag ataagagaga tacaaagaat caaggaggag
     4741 tcatatggca agttatatcg aaccgattcg ttttgcgctg attgtttttc catttttagc
     4801 cttagcgatc tcatctgttt tttttattta tgaatatcga aagtatggaa catttctagt
     4861 aacaagggca gttattttgt attctttcgt tttttattta ttgtgtgcgt attttttggt
     4921 gatcttacca cttccggcaa aggagacggt tgcccaaatg acagggccaa agatggagct
     4981 gcatttattt gcttcatggc aacatttcag agacaacacg gtattggtct tgtctgaacc
     5041 tagtacctac ctgccggcaa tgaagcagag tgtatttttg gaaccagctt ttaatgtttt
     5101 attactcata ccgtttggca tttatttaag atattatttc cggctttctt ttatgaaaac
     5161 gatgattgct agtttctgcc tttctctatt ttttgaattg acgcagctaa gtggacttta
     5221 ttttatctac ccaagaccat accgcttatt tgatgtgaat gatttagtga caaatacatt
     5281 gggtggaatc attggttttg tcgttacgcc gttatttaca tttatgcttc ctacaagaag
     5341 acgaatggat gaactttctt atgaaaaagg aaagactgtc tcattgactc gacgcttggt
     5401 cgcttatttg atcgactgga catttttatc tcttattaat ttggtagcgg cgattttctt
     5461 taaaattgcg ttcgattcag gggcagttac ttctcatatc tggtggcttt tattagaagt
     5521 aggggtatac tttatgattg taccgtttat aaccaatgga tggacacttg gcaaaaaaat
     5581 tgtgcgtatc cgtttggtgg aagaagaccg cgaacgaatt tctttaaaag cattgatgat
     5641 ccgctacggg tatctgtact ttctttttta tggtttaagc tggttggggc agttgcttca
     5701 gcaaatcatg aattcgacaa cgaatacggt gttgcagatg agtgcagtat tcatttctct
     5761 cggtgtcttg ttactacaag gtatttttat tttaaatatc ttgctatcat ttttgagaaa
     5821 aaaacggatt ctgttttatg aaaaagctag tcacacatat actgttagca cgattggcca
     5881 aacaaaggat gatgattctc gaaattagct gagcaaaaac gctttgtctg tgttataatg
     5941 ccaaagtcgt gataatttaa agagaagagg tgcacgaatg aataattttg atatattccg
     6001 cttcaaaccg tttatcaatg aagcgttaac cgcaaagggc ttcaaacagc ccacagaggt
     6061 ccaggaacgt ttgattcctt taattgcaaa aggaaaaaat atcattgggc aatctcaaac
     6121 tggtacagga aaaacacata cgtttttgtt gcctttgttt gaaaaaatca atgcagaaaa
     6181 agaggaagta caagttgtta ttacagctcc aagtagggaa ctagctgcac agatctatga
     6241 agcagctaca caaatcgcta aattttctac accggagatc cgtgtctcga attttgtcgg
     6301 cggtacagac aaacagcgtc aattaaatcg tctgcatcac caacagccac aggttgtagt
     6361 aggcacgcct ggtagaatat tagacatgat gaatgaacaa gcgctgaaga ttcacaccgc
     6421 atttgcattt gttgtcgatg aagcggatat gacattggat atgggttttt taaatgaagt
     6481 agaccaaatt gcaagtcgat taccagaaaa gctgcagttt ttagtttttt ctgccactat
     6541 tccagaaaaa cttcgtccat tcttacgaaa atacatggaa aatccaatca ttgaagaaat
     6601 caaaccaaaa gcagttattt ctgaagacat tgaaaattgg ctgatttcaa caaaaggtaa
     6661 agatgagaat caaatgattt atcaactgct gacaattggt catccttatt tagcaatcgt
     6721 atttgccaat acaaaacagc atgtggatga aattgccgat tacttgaaaa accaaggctt
     6781 gaaagtagca aagattcatg gagacatcac cccaagagag agaaaacgag tgatgcgaca
     6841 agtacagaat ctggagtatc aatatgttgt agcgactgat ttagctgcta gagggatcga
     6901 tatcgaaggt gtctctcatg ttattaatgc agaaattcca gaagatctag acttctttat
     6961 ccatcgtgtc ggacgtactg gacgtaatgg cttaaaagga acggccatta cgttatattc
     7021 gccaaatgat gagcaagcca ttgatgcgat agaaaaattg ggcgtctcat ttgaaccaaa
     7081 agaaatcaaa aacggggaaa tcgtcaaaac atacgatcgt aatagacgtg ccaaacgaga
     7141 aaaatcaaaa gatgtattag atcccacgct gattggtctt gtgaagaaga aaaagaaaaa
     7201 gatcaagcct ggttataaga aaaaaatcga ttgggcaatt gcagaaaaaa ataaaaaaga
     7261 acgcaaaatc gaacgcagac agcaaagccg tactgctcgt aaaaataaga aaaattcaca
     7321 taaataaaaa acaagaggtt gtgacaaaag gcgtgtcaca atctcttgtt tttaagctag
     7381 actttttttc attccgatac aaagtacttc atcttcaata aaaggttctg tgcaagtatg
     7441 atacccttgt ttttcataaa aatctacagc cgtcatttca gctactacat aactagtctg
     7501 acaaccttct gaaaccgccc gtttttcagc agcttcaagc aactgaccgc ctaacccttt
     7561 tcctcggtat tcttttgcta cgcaaaatcg ttccggcttg aagcaagtag gtgttttctt
     7621 ttcataacgc aaagtagcta ctggtctttt ttgatcaaga aatagataat aggtttgttg
     7681 cgggatatct tttccgtcga attctagttc tgcaggaatc ccttgttcta atacaaaaac
     7741 ttgatatctc aaaaagcagc tagctgcgcg tatccattca tccgctccaa ctattttttt
     7801 catcatattc ctccttgtca tgaatagcag agatgtattg attcctttat tcttatcgta
     7861 ccaaaaatcg tggagtatga ccaaaaggaa atgaaaataa aaattgagat attgacagtg
     7921 atcaatagaa tgcgtatact aattaagtac gaaaaggata tgatttgttt tttaagactc
     7981 acagaaaatt ctttcttggc tgaaaaaaga atagtcacta atcaaattgc tccctttttc
     8041 tattttggta gaaataccga cgcagctcag cgttatcgag tttaagaggt aatgattaca
     8101 catcattgca atcaaggtgg taccgcgagt tttcgtcctt ggctgtgatg gtgttttttt
     8161 gtatttaatt tatagaaagc aggtaatgac atgaaacaat taagtagtgc acaagttcga
     8221 caaatgtttc ttgatttctt ccaatctaaa ggacatacga ttgaacctag tgcgtctttg
     8281 atccccgtga acgatccaac cttattgtgg atcaactctg gtgttgcaac attgaaaaaa
     8341 tattttgatg gttcagttgt gccagaaaat ccaagaatca caaatgccca aaaatctatt
     8401 cgaacaaatg atatcgaaaa tgtcggtaaa acagcacggc accatacgat gtttgagatg
     8461 ctgggtaatt tttcgattgg tgactatttc aaaaatgaag ccatccactg ggcatgggaa
     8521 tttttgactg atccaaaatg gatggcattc gatccagaaa aactttatgt aacagtttat
     8581 ccaaaagata cagaagcaaa acgcatctgg catgaagaag ttggcctgcc actagatcac
     8641 attattgatg tagaagacaa cttctgggat atcggtgcag gcccatgtgg tccggatacg
     8701 gaaattttct atgaccgtgg agaagcttat aacgatttag cagaagacga tcctgaaaac
     8761 tatccaggag gagaaaatga acgttattta gaaatctgga acttagtatt ttcagaattc
     8821 aaccataagc ctgataacac atttgaacca cttcctcata aaaacattga tacggggatg
     8881 ggattggaac gcatggtctc aatcgttcaa gatgcgtcaa cgaactttga gacagacttg
     8941 tttatgccta ttatccacgc cgtagaaaag ctaagcaaac aagtaaaata tggtgaaaac
     9001 ccagtaacag atatttctta caaagtaatc gcagatcata ttcgcgccct ttcatttgct
     9061 attggcgacg gtgcattgcc atcgaatgaa gggagagggt atgtcttacg ccgtttgctt
     9121 cgtcgtgctg tcatgcatgg gaaaaaatta ggtattgaac aagcattctt atatcgttta
     9181 gtccctgttg ttggggaaat catggtaagt tattatcctg aagtacttca acaaaaagaa
     9241 tttattgaaa aagttatccg tacagaagaa gaacgtttca atgaaacaat caatgaaggt
     9301 ttggaaatct taaatcaagt gatccaagaa gtaaaaaacg aaaacgggac gactctagca
     9361 ggaaaagaca tcttcaaact atacgacaca tatggcttcc cggtagaatt aacggaagag
     9421 gtggcagaag atgaaggact gaaagttgat catgctggtt ttgaaaaaga aatggatgca
     9481 caacgcgaac gtgctcgctc tgcccgcagt actgaaaaat caatgggggt acaatctgct
     9541 ttattgacag atatcaaagt agaaagtaaa ttcattggat acacagaaca agaatcagat
     9601 agtcaattat tggtcatcgt taaaggtgag tctcttttga atgaagtcgc agaaggtgaa
     9661 gctcaaatga tctttgatca aacacctttc tatgcagaaa tgggtgggca agtagcggat
     9721 caaggatgga tcatgaatga agtaggcgaa atcgtagcag ttgttacaga tgttcaaaaa
     9781 gcgcctaatg gtcaatttat gcacaccgta gaagtaaaag caccaatgag tgaaggacaa
     9841 acctatcatc tattgatcga tcaatcgatg cgcaatcgga tcatcaaaaa ccatacagca
     9901 acccatctac tgcacaaggc attgaaagaa gtacttggcg agcatgcaaa tcaagctggt
     9961 tctttagtcg ctcctggaca tttacgattc gactttaccc actttggtca aatcacttca
    10021 gatgaattag ctgaaatgga aagaatcgtc aacgaaaaaa tctgggaagc tatcccagtt
    10081 gaaacaattg aaacagatat cgatacagcc aaaaacatgg gtgcaatggc tttgtttggt
    10141 gaaaaatacg gacatgacgt tcgagtagta aatattggcg gctggtcaat cgaactgtgc
    10201 ggaggcacac atgtagcaaa tacagaaaac atcggaatct tcaaaatcgt ttctgaatca
    10261 ggaattggtg caggcgtgcg acgaatcgag gctgtaacaa gtaaagaagc gtacgaacta
    10321 cttgaagaag aagaaaaaca actaaaacaa gtagcaacaa ttgttaaatc acctcaattg
    10381 aaagaagttg tttcaaaagc ggaacaattg caacagcaag taagagagct acaaaaggaa
    10441 aatgaacaat tagctagcaa gttagcgaat caacaagctg gtgatgtatt caaagatcca
    10501 gaaacgatca atggaaaaac aatcattgca gcacaagtaa aagtgaaaga tatgaatcag
    10561 ctgcgtcaat tagctgacca atggaaacaa aaaaatattt cagatattct tgttttagca
    10621 aacgaacaag aaggaaaagt cagtctgcta gcggcaatga cgaaagaatc aaatgaagct
    10681 ggactaaaag ctggcgactt gattaaagca atcgcaccga aagtaggcgg tggcggtggt
    10741 ggacgtccag acatggcaca agctggcggg aaaaatcctg ctggtattcc agatgcactt
    10801 aaagctgcga aagaatgggt tgcgcaacaa tagggcgttt acgtttgaca gaaaaacaaa
    10861 agttcaaaca ttgaacgaaa catgattatg ggggcgaaga caaacaagtc ttgccctcat
    10921 acttttttca attggatggc gcatctaatc aaatggtcgg attcggatgg tctttagttg
    10981 acgaaagtga cagaaatttt tacgattagt ataaataaaa aggggtgaaa aaatgaatag
    11041 tagaaataag acctttcgac tagtcttacg tgcgatcttg ttagcaatca tcatcgtaca
    11101 agcgatggtt ccttggctgg ggtttattcc attaggattc attagtttaa caattatcca
    11161 tatcacagta attattgctg cagtagtatt aggcccaaaa gatggcatgg tgattggctt
    11221 gttttgggga atcgctacaa ttgttcgtgc ctatgccatg ccaacaacgc catttgatac
    11281 attagtgttc acgaatccga ttatctcggt agttcccaga gtacttgttg gcttagtagc
    11341 aggcttggtt ttccactgga tttatcaaaa aaataaatca ataacgatca gctcagcctt
    11401 tgcaggggta ctgggttcat tggtcaatac agtcttagtg ttaggattca tgggattatt
    11461 ttatacaggt gcaacggcag atgcctatgg tgtagactct gctcttttat ttaaaacctt
    11521 agcaggggtt gcagcaatca atgggatacc agaagcaata ggtgcgggaa tcataacacc
    11581 attactagca aaagcgttat ttgcagcaac accgttgaaa ccagaataat gaccatcttt
    11641 taatagtaat aggacgcacg atagatagaa aaaggaagag aggagaggaa aacaatcgtc
    11701 ctctccatct ttttacctga gcaacaagca aactagaagg ggtaaaaaac ttggctaaga
    11761 aacaagaaga aaaaaagaaa caaataccgc aacagcggac aaaaaaagga gtactagcgg
    11821 gtatcttagg attggttacg aatctgatct tgtttgtctc taagctaatg atcgggcttt
    11881 tttcaggaag tgtctccatt atggctgatg cagtgaacag tctatcagat actgcttcat
    11941 ccatattgac tttgatcgga tttaaaattg ctgcaaagcc agcagatcag gaacatcctt
    12001 atggacacga acgcttcgaa tatatcagtg ggttatttgt ttcgatcatc attacttatg
    12061 taggatttca atttttagag tcttctgctg aaaaaatcat ccatccagaa aatgtctctt
    12121 taacacccat cgtctttttt gtcctaatca tttccatctt tttgaaattt gcacaaggca
    12181 aaatgtatca ggttattgcg caatccattg attctgaaac attgagagct acagcaacag
    12241 atagttataa tgatgtcttt actacactgg ctgtattgct ttcagcagca atcgaatggg
    12301 taactggatg gcgtattgat ggatatatag gttttgcttt agcattatat atcctttata
    12361 gtggattgac gatgcttcga aattttgtat acgaactatt aggaagtcgc ccaacgccag
    12421 cagagattga agaaatggaa gagcatttaa atgcttgcga tgatatactt ggctatcatg
    12481 atttattggt gcataactat ggacccaata aacgctttgc atcagtccac atcgaagtag
    12541 atgaatcaat gaatttgaat gtggctcata agattattga tctgatagaa aaagacttta
    12601 aaaagtcttt ggatgtggaa cttgtctgtc acttggatcc aatgcctgtt gataacgaaa
    12661 aatactataa tatcttgcgg cagttaaagc aaattcttct tgaaacaggt acagggctta
    12721 atattcatga ttttagagta attcatcaag gaaaaacgct gcaatttgat gtagtggtcc
    12781 ctgagaaatt ccagtttgtt gatacagaac tagaaagaat cttacgtagt caaatagaag
    12841 aaaaaatcgg agattaccaa ttggaaatta cgtttgatca caattatctt ttatattaaa
    12901 tgagaaacta taaaaagact ccttaaagtt tttaatttaa agagtctttt tttcttttaa
    12961 ttgtttatgg agagcctgac gtgcaagatt atctgctcct ttatttttgt tttcgggcaa
    13021 ccattgcagg atcaataaag aaaattcgga tagaagttct tggattcttt gaaggtaggg
    13081 aaggaagagc tcgttcttcg ttgcgttttt gtcgaccgta gaaactagtg ttttgctgtc
    13141 tgaaaaacag aaaatcgttt cattctgtaa gtcgtttttc tttagaaatt ctaaagtttt
    13201 taaaaagact tcgaactctg cttgatggtt tgtccctata gaaagcggga tagatagctg
    13261 ttcttgttta ccttgataga caatcaagat tccacctcca ctaggaccag ggttgccttt
    13321 tgtagaggca tcaatgtata cttttaacat aaaaagagat tctccttttt gttcaaacta
    13381 atgttatggt aagattatat cattactgaa tcggtgagag gttagagaat gaaaaaaaat
    13441 attcgatggc agcctgaact ggcacatacg atcatctatt ggtcattgac cttgatcatt
    13501 ctgtttttta gtctgattct ttctttagaa aacacaggtc cttattggaa aagcaatttt
    13561 gttttaggta tttttcttgt ttttctattt ctaggtttgc ggcgttcttt tattttaaaa
    13621 tcaaactctg tgattatacg ttatgctgct ttttggaaag accgagaaat atctgtcgga
    13681 cagattcgat ctttgacgcc aactaatact ggggtgcaaa tgatgttgca agatgagaaa
    13741 gccccatctg ttttcatgat gcgaaaaaaa gatcaagtag tcctttttga tcattttgaa
    13801 aaaaacggac ccaaatcaga atcaattcct tatccttata cgaaaaaaag aaaaagctaa
    13861 ttttaaaaat cagaacctaa aaaaacgaat ttttacagga aaagaaaaga gtgagacgta
    13921 cggttcatcc cgtttagcgt ctcactctat ttgtttatct attcatgcat actagtatcg
    13981 gtagtctgaa cgttgccttt cttgacatgg gcagcttgtg ttcctcgatt cccttcgatg
    14041 atctcaaatt cgaccacttg attttcttcc aaagttttga atccctcttc ttcaatcgca
    14101 gtaaaatgta caaaaatttc ttctgtgtca tcataactga taaagccgta tccttttttg
    14161 ttgtcaaacc attttacaat tcctgtggtc aaagcactca cgacctttct aaagtctgat
    14221 catagccttc attataaaga gtatttataa gtacgtcaaa taaattaaaa acctgtaatt
    14281 ttatgttttt tttctgaata aaaaaaggta taattggcat atcttaacaa agaagatatt
    14341 ttgttgaata aagttcgtga aaaggggtaa tcaaatgaag aatgatctgc aaatttcaca
    14401 agaaacagag ctagaaccga ttgttgaagt ggctagtaaa ttagatctta aggaagacga
    14461 tttggaactg tatgggaaat ataaggcgaa aatcaatttt tctggaatca atcgatcaaa
    14521 ggataacgct gaggggcatt tgatcttagt tacttccatc aatccaacac cagctggcga
    14581 aggaaagtca acgatcactg tagggttagg agacgcgctc aataaaatca ataaaaaatc
    14641 tgttattgca ttgcgagaac cctcacttgg tcctgtgatg ggaatcaaag gtggtgctgc
    14701 aggcggagga tatgcacagg tattgcctat ggaagatatc aacttgcatt ttactggtga
    14761 catgcatgct atcacaacag ctaataatgc attatctgct cttttagata atcatattca
    14821 tcaaggaaat gaattgaaca tcgatgcccg ccgagtgatt tggaaacggg tagtcgatct
    14881 caatgatcgt gaactgcgta aagtaattgt tggcttgggt gggccaatcc aaggtgttcc
    14941 aagagaagat gggtttgata tcacagttgc cagcgaaatc atggctatct tgtgtcttgc
    15001 ggcagacctt gaagatttaa aggctcgtct tgcaaggatc gttgtcggct atacatttga
    15061 ccgtcagcct gtgactgctg gagacttgaa agcagaaggc gctctggcat tactgctgaa
    15121 agatgcaatc aagccgaatc ttgtacaaac aatttacgga acaccagctt ttgtccacgg
    15181 gggaccgttt gccaatatcg ctcatggctg caatagtatt ttagctacca agacagcatt
    15241 gagattagca gattacgttg tcacagaggc aggatttggt gcagatcttg gcggagagaa
    15301 atttttagat atcaaagtac ctaacttgaa aaaagcgccg gatgctgtgg tgattgtcgc
    15361 cactgtccgt gcattgaaaa tgcatggcgg aatgaaaaaa gatgaactta aaaatgagaa
    15421 tcttgatgct ttgaaagtag gttttgcaaa tttaaagcgc catatacgta atatggaaca
    15481 atatcagcta cctgtgatcg tcgcaatcaa tgaattcgta acagatacag acaatgaact
    15541 cacactgttg gagcacttgt gtgaaaatca ggggattctt gccaagagag cgagtgtatg
    15601 ggcagatgga gcagaaggcg gcgttgattt agcaaaagct gtagtacgat tgattgatag
    15661 aaaagaagct aattacaagc cattataccg cctagaagaa acgatacaag aaaagacaga
    15721 aacaatcgtc aagaaaattt acggaggaaa tggtgttgtt ttcagcaaaa aagcgcaaaa
    15781 acaaatagaa gagtttacga agaatggatg ggacaaactg ccgatttgta tggcaaaaac
    15841 acagtattca ttttctgatg atccttcact gctaggtgca ccggaagatt tcaccatcac
    15901 gatccgcgaa atcatcccaa aattaggggc aggattttta gtagccttga ctggagatgt
    15961 tatgacgatg cctggtttgc ctaaaaaacc tgctgcattg aatatggacg tgacaaatga
    16021 cggagaagtg ttgggattgt tttaagcttg attataatct accatcgaag gagctgtatt
    16081 ttgactacag cttctttttt tagacgatag ggaattaatt ttgtttacac taggatcatt
    16141 ctttttcttt attgtatttt gagctatact aaagcataat aggagggtat gatgaaaacg
    16201 acatttataa gaaaaaccgg tttttacggt atgggtagct cgattcagct aagaatcaac
    16261 aatgaaaaac gcagtgttct cagtcatgaa caaaagataa cgcttgatct ggaagtgccc
    16321 tttacgatgc aggtcacctt ttattggcta aaaagccccg tttatacagt gaaagaagca
    16381 aagcctgctt atgtgatcac aatgaattac ttgattcttc aaatctatcc gtttttattt
    16441 ctagcaactg ggttgggggc cgttttccta caaaatttat ttttcagttt ttttgcagtt
    16501 attgtgttga ttgcgttctt tttctatata aaaaaccggg catatttgat caaggaggaa
    16561 aattatggca aactctgatc tttttttaat cagtttcaat cgaatcgaaa aatggatgag
    16621 agacgagatg ggaaatgcac gaaatatggg atttaccgaa cttgtcagac gcctagctca
    16681 aagaaaacaa ttaatgatca gaaaatacga agatgatctt ttacagcttg ctcagttaag
    16741 gaatgcgatc gtccatgatc ggatcgcagt cgattttgtc attgcagagc caaatgattg
    16801 gacagtcaag cgaatccagc tgatcgaaca agaactgatt cagcctgaaa ccgtgctgcc
    16861 tcgatttgca aaacgtgtaa caggttttga acaagatctt ccgttacttg aactgttaaa
    16921 gatagtagca gaaaaaaggt attcacaatt tccgttatac aataaaggaa aatttgtagc
    16981 attgattact ttacgtaata tcgggttttg gctagcaaaa gaaagccaaa aaggtccggt
    17041 tgatttaaca aataaacgag cgttggattt gatcatccaa aacggaaagt acacaaatta
    17101 tcattttgtt ccagctaaaa cacatattta cgaggttgaa gcgatgtttc gtgagcaagg
    17161 gactttggaa gccgtactta tcactaaaga tgggaatccc gatggtaatc tgctcgggat
    17221 cgtacgacct cgtgatattt ataaacaaat agaaaaggat tgattttttg ttaattattt
    17281 attggatatt cagcgctttg ctagtaggag tggatcagtt ggtcaaatgg tggatcactg
    17341 ataatctagc gcttggagag acgaaaaatc ttataccagg tattttgtca ctgaatcata
    17401 ttcgaaacac tggagccgca tggagtatgc tagaagggaa gatgtggttc tttactatag
    17461 tgacactcat tgcagtcgtt gtgattctca cattgatgat taaaaatcgt gaaaaaggta
    17521 atcgctggtt catgattgga ttgagtttga ttctagctgg tgcgattggc aattttatcg
    17581 accgtattcg tcttggctat gtagtagata tgttccagac agattttatg agctttccga
    17641 tttttaacgt agcagatgtg acacttgtat gtggagtgat ttgtatgtta atttatatta
    17701 ttttagatga aaaggatcaa agaaaaaaat gacagaactt aaaacgacga ttcaccatga
    17761 atctggtagg atcgataaag tattgactaa tttattcacc gattacagtc gttcgcaaat
    17821 tcagctgtgg ctgaaaaatg gagcggtttc tgtcaatgga cagccagtca aagcaaatta
    17881 caaagtaaaa caaggagacg agattactgt cttgatcccc gaaccagaaa cattagatgt
    17941 ggtcccagaa aatatcccgt tgacgatcgt ttacgaagat gaagatgtag cggtgataaa
    18001 caaacctcaa ggaatggtcg ttcatccttc agcaggtcat acgcatggca caatggtcaa
    18061 tgcgctgatg taccatatca aggatctttc tacgatcaac ggcgttattc gtccaggtat
    18121 tgttcaccga atcgacaaag atacttccgg actgctaatg gttgccaaaa atgaccaggc
    18181 acacgaatca ttagctcagc aattaaaaga taagacctct ttgcgcaaat atgttgcttt
    18241 agtccatgga gagattcctc acgaaaaagg aaccatcaat gcgccaatcg gccgttcgaa
    18301 ggaaaaccgg aaaatgcagg cgataagaga agatgggaag tcggctgtca cacattttac
    18361 tgtattggaa cgtttcgaag gatttactct ggttgaatta aaactagaaa ccggaagaac
    18421 acaccagatc cgtgtgcata tgaagtatat cggttatccg ttagctggag atccagtata
    18481 cggaccgaag aaaactttga aaggcaatgg tcagtttctc catgcgcaaa tgttaggttt
    18541 tacgcacccg actacaaacg aaaagatgat ctttgaagca cctcttccag aagtttttga
    18601 aaaaacttta gaaaaattaa gaaaaaacat tgcattttaa tttagataac ggtatagtaa
    18661 ttaacaacga aagcataaaa cttaatagaa ttcctttaag tttagtcccg tgaggctaag
    18721 aagggtggga gcaaggatac gtatggtgcg ccataagtag attgctcatt gacatagtat
    18781 agctatcagt gtatcagtat aagctcagtc taaacattcc tcctgtcctc ttagggcagg
    18841 agttttttta tgcaaaaaag tagggggaat gaaaatgcaa gcaaaagaag tagtagatca
    18901 ggtaaccatg aagcgagcat tgactcgaat tacttatgag attatcgaga gaaaccatag
    18961 tatccaagat atcgtattgg taggtatcaa aacacgagga atctacattg catcaagaat
    19021 tgccgaacgc ctgaagcagc tagaagatat cgatattcct gtaggagaac tggatatcac
    19081 tttataccga gatgacaaaa aagaaaatcc agaagaaccg gaacttcatt cttctgacat
    19141 cccagtttcc ttagaaggaa aagaagttat tttgattgat gatgttttgt atacaggacg
    19201 aacgattcga gcagcaatgg acgcagtaat ggattttggc agaccaagaa aaatttcatt
    19261 agcagtcctg gtcgatcgtg ggcatcgaga attgccaatc cgtgcagatt atgttgggaa
    19321 gaacattcca acatctcggg cggaagagat tttagtagag atgcaagagc ttgatggaca
    19381 agatcgcatc atgattttga aagaggaaga ttagaggaga gagaaacatg tcggagaaaa
    19441 aaggatttca taatgatgac gtggtgttag atatccacga tcgcccaacc ccaggacatt
    19501 gggtaggatt aagtttacaa cacttgttca cgatgttcgg agcaaccgtg ttagtcccca
    19561 tcttagtagg tatcgatcca ggtattgcgc tagtcagttc aggactaggt acattggtgt
    19621 acttatttac tactaaagga aagatacctg cgtacttagg aagcagcttc gctttcattg
    19681 cagcgatgca gcttttgatg aaaacagatg gttatccagc gattgcacaa ggagcagtca
    19741 caactggatt agtctatttg attgttgcgc tgatcatcaa aaagatcggt tcagcatggc
    19801 tggataaaat cttgcctcca atcgtcgtcg gtccagtcat catggttatc gggttgggac
    19861 ttgcggcaaa tgcagcaaca aatgctatgt ataatcaggg cacgtatgat ttcaagtata
    19921 ttgctgtagc gctgatcaca ttaggattaa caattttctt caacatgtgg ctaagaggtt
    19981 ttctcggatt gattccgatt ttactaggta ttattagcgg atacatcgta gccttgctgt
    20041 ttggaatcgt ggatactaag ccaatcgtag acgctgcctg gtttgctgtt cctaactttg
    20101 agattccctt tgtacaatat gaaccaaagc tatatcttgg agcaatcaca acaatggcac
    20161 ccatcgcttt tgtgacgatg acagagcata tcggccatct tatggtattg aataaactaa
    20221 ctaaacgtaa tttctttgaa gaaccgggtc ttcataagac gttgacaggt gatggattag
    20281 cacaaatcgt ggcagccttt gtcggaggtc ctcctgtaac aagttacggt gaaaacattg
    20341 gtgttctggc tatcacacgt gtccatagcg tgtttgtgat cggtggagct gcagtatttg
    20401 cagtcatttt aggattcgta ggaaaattaa gcgctttgat tttaagtatc cctggaccag
    20461 tcatctcagg aatcagtttt atcttatttg gagtaattgc cgcaagtgga atgaagattt
    20521 taatcgagaa caaaatcgat tttgataaaa agaaaaatct gttgattgct tcagttatct
    20581 tagtagtagg aattggcgga ctcgtctttg aagtaggcac atttacattg tcggcaatgg
    20641 cattagcaac tgtacttggc atcattttaa atcttatctt accagaaact tcacgaagcg
    20701 aagaaaacta aggaggaaac attatgatca tcaaatcaga acgtatcagc ttaaagcatt
    20761 tattgaccgt tgaggcactt actgatcaag aagttatggg cttgatccgc agagggcagg
    20821 aattcaagca aggtgctaat tggactccgc aaaagcgcca gtactttgct acaaacctct
    20881 ttttcgaaaa tagtactcgc acacataaaa gcttcgatgt agcggagaaa aaactagggc
    20941 tagaagtgat tgaatttgaa gcaagcacaa gctctgtcca aaaaggggaa acactatacg
    21001 atacagtact gacgatgtca gcattaggcg tagacgtagc ggttatccgt catggtgatg
    21061 agaactatta tgacgaactg atacaaagta aaacaatcca gtgttcgatt atcaatggag
    21121 gagacggaag cggacagcat ccaacccaat gcttgcttga tttgatgacc atttatgaag
    21181 aatttggtca ttttgaaggt ctgaaagttg ccatcgtagg tgatatcact cactctcgcg
    21241 tagctaaatc caatatgcag atgttaaaac gtctaggcgc acaagtattc ttctcaggtc
    21301 ctagagaatg gtacgacgat gaattcgaag tgtacggaca ttatatgcca ttggatgaac
    21361 tactagatca agttgatgtc atgatgatgc ttcgggtgca acacgaacgc catgatggaa
    21421 aagaaagttt ctcaaaagaa ggctaccatc aggaacatgg gttaacagtt gagcgtgcga
    21481 agaagttgca aaaacatgca atcattatgc acccagcacc agttaaccgt gatgtagaat
    21541 tagctgattc attagtggaa ggattacagt cgcggatcgt tcagcaaatg agcaatggag
    21601 tattcgtcag aatggcaatc ttagaagcag tgctaaatgg aaaagcgtaa ttataggagg
    21661 atgaaaaaat gaaaacactc attaaaaacg gtcagatcaa tacacgaaaa aatatgacga
    21721 caccagcaga aatttggata gaagatgggc gaatcaaagc aatcggtaca ggattctcag
    21781 aagccgaatt tgatgaagtg tttgatgcaa agggacaatt gatcacacct ggtttagtgg
    21841 acgtccatgt tcatttgaga gagccaggat ttacatacaa ggagacgatc gaagctggga
    21901 ctcgctcagc tgctcgtggc ggtttcacta ctgtatgcgc gatgccgaat ttaaatcctg
    21961 tacctgatac ggcagaaaag ctgagacaag tatatgacat cattagaaaa gatgcagttg
    22021 tgaaggtatt gcagtatgcg ccaatcacag aaaatctgcg aagtgagaaa ttagttgatc
    22081 aagaagcact gattgaagaa ggtgcttttg ctttcacaaa tgacggtgta ggtgtccaaa
    22141 cagctgggac gatgtatctg gctatgaaag aagctgcaaa aaacaataaa gccctagtag
    22201 cccatacaga agatgaatct ttattatttg gcggcgttat gcacgctggc aagaaggcag
    22261 aagaacttgg tcttcccggg atcttaagcg taaccgagtc ttctcaaatt gctagagatc
    22321 tgttgctagc tgaagcaact ggtgttcatt accatgtttg ccatgtttct actaaagaaa
    22381 gtgtccgtgt aatcagagac gcaaaaaaag caggaatcca cgtaacagca gaagtttcac
    22441 cccatcatct tattttgatc gacgaagata ttcccgaaga tttcggcttt tggaaaatga
    22501 acccgccgct aagaggacgg gaggacagag aagctttgat cgaaggcttg cttgacggta
    22561 cgatcgattg catcgctaca gaccatgccc cccatggatt agaagaaaaa agccaaagtt
    22621 tcatgaaatc accatttggt atcgtaggaa gcgaaacagc gtttcaactg atctatactc
    22681 attttgtaga aactggtcga ttcacgttgg aacaagtgat caattggttg gcagttaaac
    22741 cggctgagat ttttggtttg aatgcaggaa cgctaacggt tggtgccccc gctgatgtag
    22801 cagtttttga tattacacaa acttgcacga tcgataaaga agacttttta tcaaaagggg
    22861 agaatacacc ttttatcggc tggaaagtaa aaggcgaaac acaaatgact tttgtcaacg
    22921 gaaaacttgt gtggcagaag ggagaataaa agtatggaac gtttgcttat attagaagat
    22981 ggaacagttt tcgaaggaaa ggcttttggt gcagatgcta gtgtcttcgg agaaatcgtt
    23041 tttacaacaa gtatgactgg ttatcaagaa actatcactg atcaaagttt taacggtcaa
    23101 atcattacat ttacctatcc tatggtgggt aattatgggg taaatagaga tgattatgaa
    23161 tcgatttctc caacatgcaa aggagtagta gtaaaagaac atgcacgtgt agcttcaaac
    23221 tggcgaaacc aaatgacgct agattcattt ttgaaacaaa aaggaattcc tggaatatca
    23281 ggtatcgata cgcgcgccct tactcgcaaa atccgtgaag tcggaacgat gaaagctagt
    23341 atagttgatg ctggagattc cttcgaacat gcatttgatc agctgaaagc aaccgttcta
    23401 gcaaccaatc aagtgcagca agtctcaact aacaaacctt atccaagtcc aggaacaggt
    23461 cggaaagtca ttgtcattga cttcggcttg aaacatagta ttttacgaga attgagtaaa
    23521 agaaactgta acctcacagt acttccctat aacacagatg ccgagacgat tttagcgcta
    23581 tcaccagacg gagtcatgct gacgaatgga cctggtgatc caaaagatgt gccagaagca
    23641 atcgaaatga tcaaaaaaat ccaaggaaaa gtaccgattt ttggtatttg tctcggtcac
    23701 cagttatttg ctttagccaa cggtgcagat acctataaaa tgaaattcgg acatcgagga
    23761 ttaaatcacc cggtacgaga agtagcgacc ggaagaattg attttacttc tcaaaatcat
    23821 ggctatgcag tcgatgaagc cacgattgat ccagaaaaac tcatcgtaac tcatgtagaa
    23881 gtaaatgacg gaacagtaga aggagtcaaa cacagagatt atcccgcttt cagtgtgcag
    23941 tttcatccag atgctgcccc tggtccgcat gatgcactac atttatttga tgaatttatg
    24001 gaaatgatgg acgcaggaaa ggagcagtaa acagatgccg aaacgtacag atatcaagaa
    24061 aattatggtg attggatctg gtccgatcat cattggtcaa gccgcagaat ttgactacgc
    24121 aggaacacaa gcctgccttg ccttaaaaga agaaggatat gaagtggttt tagtaaattc
    24181 taatcctgcg acgatcatga cggataaaga agtggcagat caagtatata tcgaaccgat
    24241 cacacttgaa tttgtatcaa ggattcttcg aaaagagcgt ccagatgctc tccttccaac
    24301 tttaggcgga cagacaggcc tcaatatggc aatggagctg gcagcatcag gtattttaga
    24361 agaattgaac atagaacttc taggaacaaa gctttctgcg atcgatcaag cagaagatcg
    24421 tgatctattc aaacagttga tgaaagaatt gaatcagcca atccccgagt ctgaaatcgt
    24481 caatacagta gaagaagctg tagattttgc aaataaaatc ggctatcctg tcatcgttcg
    24541 tccagccttt actttaggcg gaactggtgg aggtatgtgt gaaaatgaag aagaattacg
    24601 tatcgtatca gaaaatggat taagtctttc ccccgctacc caatgtttga ttgaacgaag
    24661 tattgcaggt tttaaagaaa ttgaatatga agtgatgaga gattcggctg ataatgcaat
    24721 cgttgtctgc aatatggaaa attttgaccc ggtgggtatc catacaggtg attctatcgt
    24781 atttgcccct agtcaaacat tgtctgacca tgaatatcaa atgcttcgtg atgcttctct
    24841 ttcgattatt cgagcactta agatcgaggg aggctgtaat gtccaactcg ctcttgatcc
    24901 acatagtttt aattattatg tgattgaagt caatccgcgt gtttctcgtt catctgcgtt
    24961 agcaagtaag gcgactggct atccgatcgc taagctagct gctaaaatag cgcttggatt
    25021 aacattagac gaaatgaaaa atcctgttac aggaacgact tttgcagaat ttgaacctgc
    25081 attagattat gttgtagcta aaattcctcg ctggccgttt gataagtttg aaaacggaga
    25141 acggctttta ggcacgcaga tgaaagcaac tggtgaagtg atggctatcg gtcggaatat
    25201 cgaagagtct ttattgaaag ctgtgcgttc tttagaaatc ggtacacacc atatcgaact
    25261 tcctgagttg gcagacatca cggacgatga cctaatccaa aaagtggttc gagcacaaga
    25321 tgatcgcctg ttttatgtag ctgaagctat tagaagaggg tacaccatag aagaattagc
    25381 ggatttgaca aaaatcgatt tattcttttt agataaactg ttgcatatcg tagaaatcga
    25441 acagcagttg aaacagcagc cgttggatga caagatattg aaaacagcga aacaaaacgg
    25501 ttttacagac aaaaaagtgg cggagctgtg ggaaataaac gaagaagccg ttcgccagat
    25561 gagaaaagaa aatgatttga ttcctgtata taagatggtt gacacttgtg cagcggaatt
    25621 cgaatcaaaa acaccttatt tttatagttc ctatgaatat gaaaatgaaa gttcacgatc
    25681 agaaaagccc tcaattctag tactgggatc tggtcctatc cgtatcggac aaggggttga
    25741 atttgactat gcgactgtgc attctgtcaa agcaatccag caggcaggat acgaagcaat
    25801 catcatgaac agcaatcctg aaacagtttc gactgacttt tctatttcgg ataaattata
    25861 ttttgagcca ttgaccttcg aagatgtgat gaatgtgatc gagctagaac agccgatagg
    25921 agtaatcgtt caatttggtg gtcagacagc aatcaactta gccgaacctt tgactgctgc
    25981 tggtgtaaaa atcttaggga caacgatcga agacttggat cgtgcggaaa atcgtgattt
    26041 attcgagcaa gcgctgaaag agttggatat tcctcaacct ttaggggata ctgctacaag
    26101 taaagaagaa gccgtggtta tcgcttcgag aattggttat ccagttcttg ttcgtccaag
    26161 ctatgtatta ggcggacgtg cgatggaaat cgttgaaaac cagcgagatt tagaagatta
    26221 catggaacat gctgtgaaag catctccaga acatccagta ctagtggacc gttacttgct
    26281 tggtggtgaa tgcgaagtgg atgcgatttg tgacggtgaa acagtactca tcccagggat
    26341 catggagcat attgagcgtg ctggtgtcca ttcaggggat tcaatggctg tgtatccacc
    26401 tcaaacatta tctgaatcga tcaagaaaac gatcgaagat tacacgatcc gcttagcatt
    26461 aggtttgaat tgtgtcggga tgatgaatat ccagtttgtc atccatgaaa atgaggttta
    26521 tgtgatcgaa gtcaatcctc gagcaagtcg gactgttcca ttcttaagta aggtgacagg
    26581 aattccaatg gcacaaattg cgacgaaagc tattttggga gagaaactaa agacacttgg
    26641 ttatgaaaac ggactttatc cagaaacaaa agaagttcat gttaaagcac ctgtcttttc
    26701 ttttacgaaa ttgcagaaag ttgatactta cttagggcct gaaatgaaat cgactggaga
    26761 agtaatggga tctgacagac aattagacaa agctttgtac aaggcattcg aagcttctgg
    26821 attacacttg cctgaatatg gagccgtatt atttacgatt gcggacgaaa caaaagaaga
    26881 agcactagaa ttagctaaac gttttacaga aattggctat agtctgatag gaacgaaaca
    26941 tacagctgct tatttcgaaa aaaatggtct ggtcgttgaa acagtagcga aaatttctga
    27001 ggaagcagca gaaaaaaatg tagtcgactt aataagagat ggtaaaacac aagtggttgt
    27061 caatacgatt gataaagatc ggggaaatgc ttctaaagac ggtttcatca tccgaagaga
    27121 agcagttgaa catggtattc cattgtttac ttctttagac acagcagatg cgatcgttcg
    27181 agtgatggaa tcccgtgctt tttcaactaa ccctatttaa cggcgagcac atgttaagac
    27241 aaaaagacga aaaaccgttg caggagggga cagatgaaac aagaaatgat ggaaatcgtt
    27301 cagcaaaaac agctggcacc aaagattttt gaaatgacat tgaaaggtga tctagtaaaa
    27361 gaaatgcagc aaccaggaca gttcttgcat attctcgtgc cgcgtgcaga cttgctatta
    27421 cgccgaccga ttagcttgaa tcatatcgac cacgaaacaa atacttgccg tatcatttat
    27481 cgggtggaag gtgaggggac tagagtcttc tctactcttt ctgccggaga ccatttagat
    27541 gtcatgggtc ctttaggcaa tggttttgat ttgtccaatc ttcatacagg agacgaagta
    27601 tttataatcg gtggaggaat cggtgtccct cctctatatg agttatcaaa acagttagtc
    27661 gaaaaaggaa ttaagccaac gcatttttta ggttttgcca cacatgaagt cgtttattat
    27721 gaaaaagaat ttaatcgtct aggagaaaca agaatcgcta ctgacgatgg aacatacggt
    27781 atgcacggaa atgtcggtaa tttattgtta ggaacgaaga cggaacctac agctgttttt
    27841 gcatgtggtt caaacgggtt attgaagaca gtggaacagc tgttttcttc gcatcaaaat
    27901 gtccagttat cattagaatc ccgcatggca tgtgggatag gggcttgcta tgcttgcgtt
    27961 tgtcatacgc cggaagatga aacaggaaca aaaagtgtga aagtttgtga cgaaggaccg
    28021 atttttaaaa taggagaggt aattatatga atgagaatcc tttagcggtc aaacttcctg
    28081 gcctagaatt aaaaaatcct atcatgccgg caagcggctg tttcggtttt ggcaaagaat
    28141 acggaaaata ctatgatttg aatcagcttg gatcgatcat ggtcaaggca acgactccaa
    28201 atgcccgctt tggcaatccc acgccaagag tggcggaaac accatctggc atgttgaatg
    28261 caattggttt gcaaaatcct ggtctagacg ttgttatgca taaacttctt ccagaattag
    28321 aggagtacaa tgagttaccc atcatcgcaa atgttgctgg tgcttgtgaa gaagactatg
    28381 tagaagtttg tagcaaaatt ggcgaagcac caaatgtgaa agcaatcgag ctgaacattt
    28441 cttgcccaaa tgtcaaacat ggcggaattg cttttggaac agattctgat gttgcttttc
    28501 aattaacaca agcagtaaag aaagtttctt ctgttcctgt ctatgtcaaa ttgtccccaa
    28561 atgtaacaga catcgttcca atcgctcagg caatcgaagc tggaggggca gatgggttta
    28621 cgatgatcaa tactcttctt ggtatgcgga ttgaccttaa gacaagaaaa ccgattttag
    28681 ccaatcaaac aggtggactc tccggaccag caatcaagcc tgtcgctatt cgtttgattc
    28741 accaagtagc aagtatatcc agccttccaa tcattgggat gggcggcgtg cagacagtgg
    28801 atgatgttct tgagatgttt atggctggag caagtgctgt agctataggg accgcgaatt
    28861 tcaccgatcc gtatatctgt cctaaactaa ttaaagagct ccctgtgcga atgagcgaac
    28921 taggcatcga atcgctggaa agattaatca aagaagtgag agaggaacgt gaacgatgaa
    28981 tcaactagac caacgaccaa ttattgcatt agatttttca actaggcaag aggtagaaga
    29041 ctttcttcga tttttcccga aagaagaaaa attgtttgtg aaaatcggaa tggaattgtt
    29101 ctaccaagaa ggacctgaaa tcgtacgtta tctaaaagag gctggtcata atgtattttt
    29161 agatctgaaa cttcacgata ttccgaatac ggtcgaaaaa gcaatgcggg gattggcaaa
    29221 gcttggtgtt gatctcacct gtgttcatgc cgctggcggt attcgtatga tggaggcggc
    29281 aatgcgagga ttagaagagg ggactccaga aggtggaaaa cgtccgctac ttctagctat
    29341 cactcagctt acttctacaa gtgaagaaga aatgcatgcc gatcaactaa tcgaagtttc
    29401 tttagaaaaa agtgtgatcc attatgctag ctgtgccaaa aaagcaggat tagatggtgt
    29461 ggtttcttct gcttgggaag tagaagcgat caaagaaatg gcaggagacg aatttgtttg
    29521 cttaactccg ggtataagac cagaaggaac agtcgcagga gatcagacaa gagtagtcac
    29581 accttctcag gcaaaaaaga tcggctcaac ttttatcgtt ataggtcgac caatcactca
    29641 gtcaactaat ccttataaag catatcaaac gattcaaaca gaatggagct aacctaaaat
    29701 gaatgttgaa caaagtatcg caaaagactt attagaaatt gaagcagtat ttttaaatcc
    29761 aagtaatcct tttacatggg caagtggaat caaaagccca atttactgtg ataaccgaat
    29821 cacgatgagc tatccaaaag tccgaaaaga gatcgcaaaa ggattagcaa gcaaaatcaa
    29881 agaagcttac cctgaagtac aagtgattgc tggaactgca acagcaggaa tccctcatgc
    29941 cgcatgggta gcggaaatac tggatttgcc aatggtctat attcgaagta aggcgaaaga
    30001 tcatgggaaa ggtaaccaaa tcgaaggacg tatatttgaa ggtcagaaga tggttgtgat
    30061 cgaggatttg atttctacag gcggaagtgt cttggaagca gctgaagcag caaaaagaga
    30121 aggtgcggat atccttggtg ttgcggctat ctttacgtat gagttgccaa aaggaaaagc
    30181 aaatttcgaa aaagccgaaa tccctcttat gacgttgacc aattattctg tattgattga
    30241 agcagcttta gaagatcgtt atatcgatga gcaagaatta actctgctaa aagaatggaa
    30301 aaaagatcct gaaaattggc agatttaagc gcgcttgatg aagatagaga aatccgattt
    30361 gtgtctccag aatcatcaat tggaatagca aaaatccaaa aactgattgc gagaagcatc
    30421 ttttcatcag gtatgaaaag aaacttctat cgtgatcagt ttttgatttt tttaaaattc
    30481 atttttctaa gactggttag cagaaagaag agaataatct cttctctttt ttctattata
    30541 tatagatttt ctctattttt tggtaaggga gctgtttcaa aaatggtaaa gagcggaaat
    30601 gttgcgaata tcagttattt aatgatatga tattcggaaa aagaaatctc aaagggaggt
    30661 aacttttatg gggttgaatg aatttatttt aggattgaac aatttagaga cgatcacaag
    30721 ggcacctgga ttttttaaat atacagagca tacagttgca gcacattcgt atcgtgtagc
    30781 atctattgcg caagttctag gagacattga agaagcgaat ggacaaccga tcgattggaa
    30841 actgctctat gaaaaatcat tgaatcatga ctacacagag cgttttattg gtgatatcaa
    30901 aacgccggtg aaatacgcaa gcaaagagtt aagagggatg ctgcaaacag ttgaagaaaa
    30961 gatgacggac gaattcatca accaagagat tccagctgag ttccaagaag tttatcgccg
    31021 tcggttgttc gaaggaaaag atgaaaccgt agaaggagaa ctattagcca tttcagataa
    31081 ggttgacctt ctttatgaat cttttgatga aatcatcaaa aataacccgg aaaaagttta
    31141 taaggagatg ttcattgagg cagtgactac gattaaaagc tttgaacatc ggccttctgt
    31201 tcagtatttc tttaaagaga tatttccgga actattgaac caggaatttt atggaaaaga
    31261 ggcgttttta gaaaccgtct caacgattgt aaagaaagaa gaaaaataaa aatgatatga
    31321 gatgcagggg gaagtatagt tgaaaaagaa tatggatgtc gaatggggct attctggaga
    31381 caaaggtccg gaaaattggg cgagtttatg tgattggttt gcgagaggtg cggaatttcc
    31441 attgcagtcg cctattcatt tgaccaaggg ggaagaaacc aaaatagaag gagacacgct
    31501 ttcctttcat tatcaaaagc aacggtttac tgacaaggaa ttcaaaaaca cgattcattt
    31561 agtacctttt gaccaactca gttatgtaga gttcaagaaa gaacgctatt atttgacaga
    31621 tattcatttc cacttgcctt ctgaacatat catcaacgga aaacaggaag atattgaatt
    31681 tcattttgtt catatgaaca gcaaaaaaga gaatctagtt gtgggtgtca tgtaccagct
    31741 aatggtacag gaaggttggc tgtataatca agaaaatggg gaaagttgga acttagaaaa
    31801 acatgagcat tgggtaaatc cggatgtgtt ctttccggag caaaaaagcc atttccatta
    31861 tattggctca ttgacaactc cgcctaccag cgggccaatc caatggtttg taatggatca
    31921 tgtaggaaaa ctgaaccaag agtttcttct ccccttcgat ggtcagtatg ctcgaccgaa
    31981 caaccgacca atccagccct tgaatggaag agaaatttat tatttcgagg agtttgagca
    32041 atgaatattc agcagatgaa atacgtggtc gctgtagcaa acaatggtag tttcagagaa
    32101 gcggcaaaga aaatgtttgt ctcccagcca agtctttcag ctggtatcaa agaattagaa
    32161 accgagttag ggatcacgct gtttactcga acgaatcgag gggcatattt aacagaagaa
    32221 gggcaagact tcttaaatcg tgccgagaaa atattggtcc agcttgatac attagaaact
    32281 cactatctaa cgagtgaaaa gagagacaat ttttcgatcg ctgcccagca ttatgatttc
    32341 ttaggtcctt tgaccgcaca gctactcgag gaattcaaag acgagatcaa acaatttcgg
    32401 atcatggaga cgacaacagc gagagtgatc gaagaagtca aagagcagca cagtgagatc
    32461 ggcattattt atatgaatga gcataatcaa gctggtatca gaagatattt acaacaagga
    32521 gagctaagtg gccgcagcat tggaaaattc aaaacgcata tttttttaag gcaaggacat
    32581 ccattagctg aacacgcaga gatcaccaaa gaagaattgt tatcttatcc tcaagttcgt
    32641 tttacacagg atggaaataa tttcccgtat ttttatgaag accttatcga gatccctgat
    32701 caagaaacag tgatttatac gagtgatcga ggtactttga tgaatatcgt tcttgaaact
    32761 gatgcttacg cttcaggatc tggtattgtg attggaggga ttcgcgagca cttgcggtta
    32821 atccctttgg cagaaggcga actaaatgaa ttttatatca tttattcggc gaaacgtccg
    32881 ctcagtgaaa tcgctcaaag atttatcaaa gagcttactc ggttactgga ccagcagagc
    32941 taagaaaagc agatgagaac gctgtttatt gaacaccgtt gatcaaaaaa gaggggttgt
    33001 gacagaattt tgtcacaact cctctttttg tagcaaacaa tttataacta attgctgttg
    33061 tgggaaagta cgcaaatatg attctagaaa cactcattga ttacctgttt tgtttcatag
    33121 ctgtaatttt ttcttcttct ggtgtcacga taatcgtctt ttggttttca taaataacat
    33181 aaccaggctt ggcgccattt ggttttcgga tatgctttac ctgtacaaga tcaaccggca
    33241 cttgggcaga gtatcggtat tttgaaaaat aggcagctaa ctcggcagct tctgtaattg
    33301 tttcatctga gggcttgtca cttttaatga tgacgtgcga accgggtata ttttttgcat
    33361 gaagccaata gtcggttttt ttagctgtct tcattgtcag ctggtcattt tgaagattgt
    33421 ttttaccaac aagaatgagc gttccgtcag aagaaaagta ttgatcaggc tgagatggtt
    33481 tcttacgttt ttgttttttt aagttcttct ttttcaggta tccttctgaa gtcaattctt
    33541 ctttaattgc ttcgatatcc attgggccgg caatttccag ttgagataag acagattcaa
    33601 gatattggat ttcatctttg gcttcttgga tttgttcgcc aatcagctta acagcgttct
    33661 tcagcttttg gtatcgatgg aaatatttct gtgcattttg atttggtgtc aatgccggat
    33721 ctagtgctat tttgattggc cgatcttctt cataataatt aggtaaaaca acttcttttg
    33781 cgccgcgtgg tacttgtgtc agaaaagtag taagtagttc gccatttcta cggtaattct
    33841 cagcgttttc ggattctttg agtgtttgtt cacgtttttt caatttattt ttatttcgtt
    33901 ttagttcatt ttcgattttt cgaatcagtt ctccgccttg ttgttttgcc cgatctttct
    33961 ctgctttttc gtgataatag ctatctaata attgactgag tgttggataa gctgttacag
    34021 cagatgcctg ttcggacaat acctgataag caatcggtgt gaaaaaatct ttattttccg
    34081 tttcgtaaaa agtaggaata ggctgatggc tgatggctga gaaaaattcg tcccatacaa
    34141 ccatcttttc attaggacgt tccagtaatc ttttggtcag ctcttgagcg atatcgaaac
    34201 cgattccctg aaactgacgt tggattgctt ttggatctag ttcagtttgg gaaagccgct
    34261 gaaagatttt ttctttctcg gagctaaatg ggttcaagac ctcttgtttt ggcggagcaa
    34321 catattctac accaggaagc aaagaacgat aagtgttttg agagcttcca atgtgtttga
    34381 tcgcatccag tatttttcca gtttctcgat taactaagac gatcgtacta tgacgtccca
    34441 ttaattcaac gatcaatatg atattttgta gatcacccaa ctcgtttcgt ttagcaaagt
    34501 ggaaatggat cactcgatca ttttcgattt gttcgatgtc ttctaaaata gcgccatcca
    34561 aatattttcg aagcatcatc acgaagttag gcggattatc aggattctga taatcgattt
    34621 gggtaatctg tacacgagca taacttgggt gcgcagataa taataatcga tggtttttgc
    34681 cgcgtgaacg aatcaccagc acgatttcat tttcataagg ctgatggatt ttcgagatcc
    34741 ggccagatag taacgtttca cgcaattcgt tgaccatcgc gtgggtaaat acaccatcaa
    34801 aggacatggt tcattcctca tttcttttca taagtatatt cattataacg aaaaaaagaa
    34861 gaaaaaccaa tttggccgaa tagtaaagca aaagtctaga aaaatcttgg actaattaca
    34921 taaaaaacag tgtttctatc gttgaaatcc aaatttttag gtataataaa aaataggaga
    34981 aaaagtagaa aagaaggtgt ttgttatttg ggaagtagtt aaatcgagag tacaagaaat
    35041 gaatgaacat ttctcagtgt cattcgcaga aaatccaagt aagtattttc gacgtcttgt
    35101 ttttctgctc atcttatcat tgctgttaac agttgcaatg tttttattgc cggaaaaaat
    35161 ccgagaagag caagtaaata gtctcgttta ttcgtccata ccaaccagca aattgaaacc
    35221 ttttgattac agcaaagctg atcagaaaat caaacaaagc cgggcgatat cggtgatgtt
    35281 cagcaaaccg aacgggaaaa cctatcagga catgcttgat gtttttaatg atcctgagct
    35341 tatggaagaa ttgaatcgac cgatctttta ttatccgatc gtttatgatg tacatgaact
    35401 ggaacaaaaa tataacatac gaacggatga agtgacattt attttctttg atggaggaaa
    35461 agaagcaaat cggataactg cgggtaaggg aggaataact gattttgata aggagttgat
    35521 ccctgaactg aatcgacttc cgttagccaa tatcaaacaa ttagaagaag aattggatag
    35581 aacgggagta acttcccaaa attccgagat tgctaattga gacattggcg cgatgaaacg
    35641 gtgggtctat atagcgctat ttttaattat cagaacaagt gatgtttgat cttcaaaata
    35701 acaaataaaa aattaaaaaa tatagttgac gaaattcaaa aattcgatta gacttaaatc
    35761 aattcttaag gaaagggtga cctgtaatga acttatcaaa acgtatttca gcagaaaaca
    35821 aaaaaaattg gcgcagctgg cgtagataag ttgtattaat ggtgagactt tcccatagat
    35881 acaacttttt agagtgcatt tctaaagctt gtatctatgc tagacatgaa cattgactgc
    35941 cgcatagatt agaatctatg cggttttttg tgtaaagaca atgaccgctt tggccattgt
    36001 ctttttttgt agactggata cttgcctgat tataataaat aaaaagattg actttgcagg
    36061 aggaaaaaaa tgaaaaacaa acgattaata acagttgtag taattatttt agtttattta
    36121 gcaggtgcat ttatttatgg aaaagcctca ggcgaaaaag aaagtacttc gacgaaccag
    36181 acaaaaaaaa caacagttgt tggggtattg caatacgtga gccatccggc attagatgag
    36241 atttatcgag gaatcaaaga tggtctgaaa gaagaaggat taaaagaagg agaaaatctt
    36301 gagatcaaat ttcaaaatgg acaagccgat cagagcaagc tagccacaat gagtcaacag
    36361 ctagtacaat ctgatccaga tgccttgatt ggaatcgcaa cacctgccgc tcaatcatta
    36421 gcaaacacga cttcatcgat cccagttgtg ttaggtgctg taacggatcc tgtgggggct
    36481 ggattagtcg cttctttaga gaaacctggt ggtaatgtaa caggtgtttc tgatcagccg
    36541 cctgttactt cacaaatcaa gcttgcttct gatttgttgc caaatgcaaa aaaagtcgga
    36601 atcttgtatg cttcttcaga ggataattca aaataccaag tagcacaagc agaagaagct
    36661 gcaaaaaaag aagggttaga atcagtaaaa tatgcggtgc cttccactaa tgagattgca
    36721 caaacggttc aagtgatgag ccgacaagtc gattttatct atgttccatt agataatacc
    36781 attgccaatg cgatgcaaac agtggtaaaa gaagcaaaca aagcaaatat ccccgtcatt
    36841 ccatctgtgg atacaatggt ggaacaaggt ggactagcaa caatcgggat caatcaatat
    36901 caattagggc ttcaaagcgg taagatggcg gcagaacttg cgagtggaaa agaaaaaccg
    36961 gaaacgacgc ctgtttatac atttgatcaa ggggatacag tgatcaatca atctcaagct
    37021 gatcgcttag gaatcattgt tccccaatcg atgaaggaaa aagccaaaat cattacagat
    37081 gactcgcagc aagaaacatc taaggaggat gacaaatgat cgtatcagct atttcacaag
    37141 gcatgctttg ggcaatatta ggattaggga ttttcatgac ttatcggatc ttagactttc
    37201 ctgatatgac gacagaaggg tccttcccat taggtgctgc tgtctgtgtg acagctatta
    37261 ctcatggggt agcgccgatt gtagctacat tattaggcgt aggtgctggc atgatagcag
    37321 gactcgttac ggggttactt tatacaaaag gaaagatacc ggttatttta gcagggattt
    37381 tagtgatgtc aggattgaac tcagttattt tgtttgtgat gcgaacacca aatctttctt
    37441 tattgaatca tccgattcta caagacagct tccatcgctt tgggttacct gattactttg
    37501 acacagtcct tcttggcttc atctctttgg gcatcttgat tgctgtactg ctattcttct
    37561 tcaatacaga tttgggtcaa ggctatattg cgacaggaga caacgaaacg atggctcggt
    37621 cgttagggat aaaaaccgat cgtatgaaaa ttttaggttt aactttgtcg aacggtgtca
    37681 ttgcattatc cggtgcgctg attgcccaga acgacggata tgcagatgta aataaaggga
    37741 ttggcgtaat cgtcatcggt cttgcttcca tcatcatcgg tgaagtgatc ttccaagaat
    37801 tgacgctagc agaaagattg attgctattg ttgtcggcag tatcatttat cagctattga
    37861 ttttagtagt aatcaaacta gggtttgaca cgacttatct gaagttgttc tccgctatca
    37921 tcttagctat ttgtctgatg atcccgcaat tgaagaaagc actgaactta aaaacgggtt
    37981 ttgagaagga ggcatagtaa tgactgatat attgacaatc aaaaatggca gaaaagtcgt
    38041 aaacaatgga atgaatgaag agaaagtact attggatgaa attaacctta caatcagaga
    38101 aggggacttc atcactgtcc ttggcgggaa tggtgctgga aaaagcacac tgttcaatac
    38161 gatcgctggt acgcttcaac tcagtcaagg atcgatctct tttaaagatc agctgatcac
    38221 aaaagattca gaagaaaaaa gagcacgatt cctttctcga gttttccaag accccaagat
    38281 gggaacggct cctcggatga cagtagcaga aaatttgcta cttgcacaaa aaagaggtca
    38341 aaaacggaaa ttccgtttac gtaacttgaa aaatcaaaaa gaagattttt tcagactatg
    38401 tcaagaagtc ggtaatggcc tagagcaaca cttggatact ccagcgggtg aattatctgg
    38461 tggtcaacgc caagcattaa gcttgctgat ggcgacgatc acagctcctg agttactatt
    38521 attagatgaa catactgcag ctttagatcc taaaacagct aaagcattaa tgcgattaac
    38581 aaatcagcgg atcaatgaac aaaaattgac ttgtctcatg atcacgcatc gaatggaaga
    38641 cgccttgaaa tacggaaatc gtctgatcgt actccaaaaa gggaaaatcg ttcaagatat
    38701 ttctgaaaaa gaaaagcaac atttgacaat gggcgacctt ttgcagttct ttgaagaagt
    38761 agtggattga tttcgacaga aaaattcctt tcagtagaag gacttgaaaa ctcattgaca
    38821 tcttcctttg attcatccta tacttaaaac aataaacaat taaacatatt tgggtgtttt
    38881 gtaggatcac gtagtcaatg attttaacaa ttaaatagaa tattctttgc tctgacaagt
    38941 aaacgatgag aaaagaattt gctactttgc cacctagacg tttcgtactt tttcgtatca
    39001 aacgctaagt ggcttttttt attgaagtaa gaacaaagga ggaataagat gatttctgga
    39061 caaaccaaat tagctgggct ctttgctgat ccggcttctc acagtttatc cccattgatg
    39121 cacaatactg cttttgaagc aaacaacatc gatgcggtgt atttagcttt ccaagtaaat
    39181 ccaatgaatt tgaagcaagc agtcgaaagt attcgaactt ttgatatgtt aggtgtaaat
    39241 ctttcgatgc cgaataaaac agcggttatt ccttatctgg atgaggtaag ccaggaagcg
    39301 caattgatcg gagcagtgaa tacgatcgtg cacgaaaagg gtcgtttgat tggctataat
    39361 actgatggtg cgggcttcat gcaatccgtg caagaagctg ggatttcgat caaaaagaaa
    39421 aaaatgactc tactgggagc tggaggtgca gcaaaggcag tcactgttca agcagcgtta
    39481 gacggcgctg aggaaatcgt agtttataaa cgaaacaacc aaacattcgc tcaagtaaaa
    39541 gaaacattcg aacaactcag ctatcgaact tcttgtaaga taaaagttcg tgactttgcc
    39601 gatacgcatc aattaagaaa agacttgaaa gaaagctgtc ttctagtcaa tgcaacagat
    39661 atcgggatgg gaaagaaaat caatcagtca ccgctcttag atataagcat gatgcatgag
    39721 aatctagcgg tttttgattt gatttatgca cctagagaaa cacgtttatt aaaagaagca
    39781 aaaaaagtag gagcaaaagg atacaatgga cttggcatgt tgatctacca aggagcaatc
    39841 gcgtttgaat tatggacaaa ggaaaagatg ccgatccatg tgattgaaaa gctgtttgat
    39901 taaaaaagga taatagataa agatctttta ttctcgcata aacggtctca agaaaagtaa
    39961 tcctttatgt atcgaagcat ttatttcgtt attttttatt aaattatcaa aaaatgatag
    40021 gcttttgtcg attggttatc gtatccttta tttatcagta agaaacgagg aggaaccaca
    40081 atgagtcgga tggatcgcta taaggacatc cataagaagg cgaaacctct gaagcgggat
    40141 acggggagta tttttgctag aagagaacga aaacgaacgc gtatggaaaa tgaggaagat
    40201 gctacccgta tttatgaaag agagcagcat tcatttgaac atttgaacca atacgagcaa
    40261 cagatataca gagatgcacc aagagcagaa gaacaacaaa aaaataaaaa atttttcaat
    40321 cggcaaaaga agaacaaacc gttaaaacct cgaagaaagc gatcgtggaa aaaaatcatt
    40381 ctatcgattt tgcttgtttt gatcctttat tcaggtatct cattttttat tggtaaatat
    40441 gtagctgaac atgacacaag cttagaggca gcgacttcag aaacctttca tgggcaaaaa
    40501 gcgacgaatg gtgcgcataa tattttgatc cttggaagtg atacacgcgg agaagatgcc
    40561 gggcgagccg atacgatcat ggtcttacag ttggatggtc cttcccataa gccgaaactg
    40621 atttctttta tgcgtgacag cttcgttacg atcccagggg taggacaaaa taagatcaat
    40681 tcagcttatg catacggagg agccgatcta gtgcgtcaaa ctttagtaga aaattttgga
    40741 atcgattgtc aatactatgc gaaagtcgat ttcaaatcgt ttgaaaaagt gatcgatgcc
    40801 ttattcatga acggagtgaa gatcgatgca gaaaaagatc tgaatctgga tggtgtagat
    40861 attaaaaaag ggggccagaa gatggatgga catgttcttt tgcagtacgc acgtttccga
    40921 atggatgaac aaggagactt tggccgtgtc agacgccaac agcaagtaat gaacgctatt
    40981 ttcagccaat tgaagaatcc attgaatctg atccgtgcac cttatgcagc tggtaaagct
    41041 ttaggatata catccacaga tgtcactgct tggttcttga cgaaaaacct cttttcgatt
    41101 gcacgaggag ctggtggcat tgatcgaata agtgtgccag tagacggttc gtggaattat
    41161 gggcaaagtg attatgctgg cagtattctt gaaattgata atcaaatgaa tcaatctgct
    41221 attacagatt ttttgaataa atgatcataa aaaagatcaa gaagaagctg cgcaaggctt
    41281 ttgtcatgaa tcaatcaaac gattcactcg taaaaaacgc tttcttatgg tagatttaac
    41341 caagagagga gctgtttaca ttggatatct atctgaactt caaaaatgag tcgcgtgaag
    41401 ccatcgcttt ttatgaaaaa gtttttcaga ctgaagcatc aggtatcacc acatacggtg
    41461 aaatgcctgg aatagaacaa atgaacttgc cagaagaagc aaaaaatcta atcacgaatg
    41521 cacggctgaa catttatgat acattagtta tgtttgcaga tacgccagaa ggaattggtc
    41581 ctgaattagt tgtgggaaat aatgtttctt tagtcatcca gacaagaaat gaagaagaaa
    41641 cgaagcgcct atttgaaagc ttagctgagg gtggtaagat tgagatgcca ctggatgctg
    41701 ttccgtgggc tcagttgtat gggatggtaa ctgatcgatt tggcatcaac tggcagatta
    41761 accaagagtg acagctaaag atgaatattt tctggaaaca tcaagcagca aacggatcat
    41821 cgtttgtttg cttgatgttt ttgtgtctat ttcgactata cggtatcttt tttttattgc
    41881 cagatgcgaa aatatgttcc ataatagaga agtccgcaaa aaattaagta acggaggtca
    41941 gaagatgaag acggtgatac tagctgaaaa accttcacaa gcaaaagcct atgccgaaac
    42001 cttccaacag caaaagcggc atgaagggta ttatgaaatc agcgatccat tgtttgctgg
    42061 tgaagtaacc atcacttatg gttttggaca tttggtagat atggttgctc caggagctta
    42121 tgaagagcgt tgggcaaaat ggtctttaga aaatttgcct attttcccag aaaccttccg
    42181 ttacgaagtt ccaatggaca aacgtgcaca attttcgatc gtaaaacgag aattgcagtc
    42241 agccgatacc atcattattg caacagatgg tgatcgagaa ggtgaagcca tcgcgtggtc
    42301 gattattcga caagcaaacg cattttctaa ggaaaagaac tatttacgtt tatggatcaa
    42361 ttctcttgaa aaagaagcaa tctatgaagg ttttaagaat ctccaacctg gaaagaacta
    42421 ttttccaaaa tataaagaag cgcaagcaag gcagaacgct gattggctga ttggaatgaa
    42481 tggaagtcca ttatatagct tgctacttca acgaaaaggg attgatggca gtttttctct
    42541 tggtcgtgta caaacaccga cactctatat gatttatcag ctgcaagaag aaatcaaaca
    42601 cttcaaaaaa gaaacgtact ttgaaggaga aggtttaatt acgacaaaca aagggcagtt
    42661 ttctggaaaa atcgtaccaa aaaaagcatt taaaacccaa gaagagttaa cgaaggaaat
    42721 agaaaaactt ggagcgcatt taggtaagca aaacggacgg attcttgaag ttacgaaaaa
    42781 ggaaaagagg ttgcatagtc cacgtttatt ttcgctatct agtttgcaga caaagatgaa
    42841 tcagctaatg aaagcaagtg ccaaagctac acttgatgct gctcaaggat tgtacgaaag
    42901 caagtttcta agctatccaa gaacggattc cttttacatt acagaaaatg agcatcagta
    42961 tctggttaaa catctgcaaa attataaaaa atttttagga atagaagaag tacagacaac
    43021 tgagatcaaa gcaaagaaac gatatgtcga tgcgaaaaaa gttcaagaac atcatgcaat
    43081 tattccgaca aagacgattc cgactcctgc acgctttgcg aagctttcca aattacagca
    43141 agcaatctat ttgcaagttt tgcgcactac tgtggctatg tttgctgcag attatatcta
    43201 cgaagagacg accgtaatga cgggcgtaca gcaattaaag ctaaaaagca tcggaaagat
    43261 cccattacag caaggttggc aggaaatctt acgcatcaag tcaaaaaaga aagaagctca
    43321 gactcttcct cctatcgtag aaaatgaaga agcagaagta gaactgaaaa gtatggagaa
    43381 agaaacacaa cctccaaaac cctataccga aggaacgttg atcacagcaa tgaaaactgc
    43441 gggaaaaacg ctggataatg atgaagcgca agaaattctc aaagaagtag aaggaatagg
    43501 gacagaagct acacgagcca atatcattga aaatcttaag cagaagcatt acatcgaagt
    43561 caataaaaac gaaataactg ttacgccaaa aggaatcact ctttgtaagg cagtagccaa
    43621 tgaaccatta ctgacgagcg cagaaatgac tgcgcgctgg gaagggtact tgagaaaaat
    43681 tggtaaacaa gaggggacac catctgtttt tttagagaat atcaaaaaat tcatcctaca
    43741 tttattggca gaagttccta atcaaatcga caaggtagac ttttcagatg agattagcgg
    43801 gacacgtcag ataaaagcga tggagaagaa aaacgatcaa ttaggaagat gtccaaaatg
    43861 taaaaaggga atagtcatgc tttatcctaa aatcgctaca tgcttgaatc cggaatgcga
    43921 cttcaaattg tggccaacag tagctaaaaa gaagctgact aaaacgaatc tcagagattt
    43981 gctttcaaaa ggaaagacaa gtaaagttgt caaaggtttt actggtaaaa aagggaaatt
    44041 cgatgcagtt ttagtattaa aagaagatat gacaatcggt ttttcttttc catggatcga
    44101 aaacagtgga acaaaagcag ttgatggaaa caaagaaaca tcaagaaaaa ataatgaaaa
    44161 cgaagtagag taaatgaaaa caatgaaata gcggaatatt tattccgaaa agtgagaata
    44221 taaataccaa taaaactaaa aaatatcagt gaatcaagca aaaacgttgg ggtaggctga
    44281 ttttatatag atagcaagga gtttttttat ttgaaaaaag tgcattcatt tttaaattat
    44341 cttatgggag caatcacgat tgtggctgtg taccaagcat ttctaaagta tcctgattac
    44401 gggacgaagt tgttgtcatt gacgcacaat caaacagcat attcaccaac gttgatttta
    44461 tcgactttga ctgcaggagc taaaagcgct acttattttt ttcataaaac gacaagcatg
    44521 ctaaagcgaa agaaacagcg aaaacaattg ttggtggtgg tagcggctat gatcattatt
    44581 tattttctac cagttattac acaagtattt catcaatatt ttggttaaga atacgagagt
    44641 ttgtgacaaa agtcatgtca caaacttttt tctttgtcat tttttcgtaa gaaaaagcaa
    44701 aataaaagta tatacattga ttaaaagtat atacttggat ataatgtact tactatagaa
    44761 tgagaatggt atagagggtg gcgaaatatg aaaaaaatga aaaagtataa aattagaaaa
    44821 tctggcaata gcgatgtaac aactatccca ccagaagtaa aagaattcat gggtgtgcag
    44881 acaggtgacg caatcagtta tgtttttcaa tctgatggtt ctgtacgaat gatcaaagca
    44941 caagaagaac ctgatattga ttcattagtt gattcgatca tgaatcaata tgaagacgcg
    45001 ttaaaagatt tggttgattt atgaggtatc tttcgactaa agaaattatc aaaatcaacg
    45061 cgaaagttat tttaccgtca ctcgccaggc gaaatgatcg gtgtaaaaga tgcaaatgca
    45121 ttagatatgg ccgtcaaaca gccttcacaa gcagtattca atcaagaact ctatcctgaa
    45181 atctatgaaa aagcggcgat tttagcaatt aatttagcta aaaaacatcc ttttcataat
    45241 ggcaataaac gaactgctct tgttgccatg ttgttgtttc ttcaattgaa taattgtcca
    45301 gttgcttttt ctcgtcaaga agctgttgat tttatcataa tgattaccac tagttcattg
    45361 gatttcgatt cactaaagtc caagataacc agttatttac gacaaaccgc aataaaatga
    45421 acgaattaaa aactttattt ctcaattaga tcagcatgtc attaaggaat at
//
LOCUS       DAZKCC010000015        44883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000015 DAZKCC010000000
VERSION     DAZKCC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.015"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8VH_000864"
     CDS             212..604
                     /locus_tag="ACG8VH_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9109441.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8VH_000865"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8VH_000865"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP9109442.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8VH_000866"
     CDS             2698..2943
                     /locus_tag="ACG8VH_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109443.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8VH_000867"
     CDS             3165..4775
                     /locus_tag="ACG8VH_000867"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP9109444.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8VH_000868"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8VH_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109445.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8VH_000869"
     CDS             5067..5936
                     /locus_tag="ACG8VH_000869"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9109446.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8VH_000870"
     CDS             6101..7372
                     /locus_tag="ACG8VH_000870"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9109447.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8VH_000871"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8VH_000871"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9109448.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8VH_000872"
     CDS             8763..9026
                     /locus_tag="ACG8VH_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9109449.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8VH_000873"
     CDS             9471..9920
                     /locus_tag="ACG8VH_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9109450.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8VH_000874"
     CDS             9977..11395
                     /locus_tag="ACG8VH_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9109451.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8VH_000875"
     CDS             11395..12255
                     /locus_tag="ACG8VH_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9109452.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8VH_000876"
     CDS             12429..13202
                     /locus_tag="ACG8VH_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9109453.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8VH_000877"
     CDS             13349..13714
                     /locus_tag="ACG8VH_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP9109454.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8VH_000878"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8VH_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9109455.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8VH_000879"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8VH_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109456.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8VH_000880"
     CDS             15560..16216
                     /locus_tag="ACG8VH_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9109457.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8VH_000881"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8VH_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9109458.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8VH_000882"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8VH_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP9109459.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8VH_000883"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8VH_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9109460.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8VH_000884"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8VH_000884"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9109461.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8VH_000885"
     CDS             21888..22202
                     /locus_tag="ACG8VH_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9109462.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8VH_000886"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8VH_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9109463.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8VH_000887"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8VH_000887"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9109464.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8VH_000888"
     CDS             23546..23875
                     /locus_tag="ACG8VH_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP9109465.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8VH_000889"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8VH_000889"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9109466.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8VH_000890"
     CDS             24863..25687
                     /locus_tag="ACG8VH_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP9109467.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8VH_000891"
     CDS             25680..26027
                     /locus_tag="ACG8VH_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP9109468.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8VH_000892"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8VH_000892"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9109469.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8VH_000893"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8VH_000893"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9109470.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8VH_000894"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8VH_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP9109471.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8VH_000895"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8VH_000895"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9109472.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8VH_000896"
     CDS             31684..32451
                     /locus_tag="ACG8VH_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109473.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8VH_000897"
     CDS             32441..34483
                     /locus_tag="ACG8VH_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109474.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8VH_000898"
     CDS             34764..34913
                     /locus_tag="ACG8VH_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP9109475.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8VH_000899"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8VH_000899"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP9109476.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8VH_000900"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8VH_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP9109477.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8VH_000901"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8VH_000901"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP9109478.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8VH_000902"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8VH_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP9109479.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8VH_000903"
     CDS             39330..40412
                     /locus_tag="ACG8VH_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP9109480.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8VH_000904"
     CDS             40412..41215
                     /locus_tag="ACG8VH_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9109481.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8VH_000905"
     CDS             41285..42025
                     /locus_tag="ACG8VH_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9109482.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8VH_000906"
     CDS             42036..43040
                     /locus_tag="ACG8VH_000906"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP9109483.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44883)
                     /locus_tag="ACG8VH_000907"
     CDS             complement(43085..>44883)
                     /locus_tag="ACG8VH_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109484.1"
                     /translation="RIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFY
                     TKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKI
                     LYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKFYLGTTGPLI
                     SFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVS
                     EDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGV
                     LLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIH
                     CLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDL
                     LEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLC
                     SFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRET
                     HAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKD
                     VADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14428 a         7831 c         9344 g        13280 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa atttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gta
//
LOCUS       DAZKCC010000016        42291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000016 DAZKCC010000000
VERSION     DAZKCC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.016"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG8VH_000908"
     CDS             6..245
                     /locus_tag="ACG8VH_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109485.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG8VH_000909"
     CDS             312..521
                     /locus_tag="ACG8VH_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109486.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG8VH_000910"
     CDS             581..1252
                     /locus_tag="ACG8VH_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9109487.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG8VH_000911"
     CDS             1305..3281
                     /locus_tag="ACG8VH_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9109488.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG8VH_000912"
     CDS             3294..4049
                     /locus_tag="ACG8VH_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9109489.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG8VH_000913"
     CDS             4046..4870
                     /locus_tag="ACG8VH_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9109490.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG8VH_000914"
     CDS             4880..5128
                     /locus_tag="ACG8VH_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109491.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG8VH_000915"
     CDS             5171..7270
                     /locus_tag="ACG8VH_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFP9109492.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG8VH_000916"
     CDS             complement(7308..9365)
                     /locus_tag="ACG8VH_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFP9109493.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG8VH_000917"
     CDS             complement(9479..9691)
                     /locus_tag="ACG8VH_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109494.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG8VH_000918"
     CDS             9878..10345
                     /locus_tag="ACG8VH_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109495.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG8VH_000919"
     CDS             10342..10584
                     /locus_tag="ACG8VH_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFP9109496.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG8VH_000920"
     CDS             10602..10904
                     /locus_tag="ACG8VH_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109497.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG8VH_000921"
     CDS             10976..13030
                     /locus_tag="ACG8VH_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109498.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG8VH_000922"
     CDS             13030..15642
                     /locus_tag="ACG8VH_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFP9109499.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG8VH_000923"
     CDS             15639..18170
                     /locus_tag="ACG8VH_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP9109500.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG8VH_000924"
     CDS             18222..18554
                     /locus_tag="ACG8VH_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109501.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG8VH_000925"
     CDS             18555..19148
                     /locus_tag="ACG8VH_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109502.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG8VH_000926"
     CDS             19311..21005
                     /locus_tag="ACG8VH_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9109503.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8VH_000927"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8VH_000927"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9109504.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG8VH_000928"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG8VH_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG8VH_000929"
     CDS             24078..25283
                     /locus_tag="ACG8VH_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9109505.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG8VH_000930"
     CDS             25299..25943
                     /locus_tag="ACG8VH_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109506.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG8VH_000931"
     CDS             25954..26760
                     /locus_tag="ACG8VH_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109507.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="ACG8VH_000932"
     CDS             26782..27012
                     /locus_tag="ACG8VH_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109508.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="ACG8VH_000933"
     CDS             27558..27833
                     /locus_tag="ACG8VH_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109509.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="ACG8VH_000934"
     CDS             27875..28297
                     /locus_tag="ACG8VH_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109510.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8VH_000935"
     CDS             28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8VH_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFP9109511.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="ACG8VH_000936"
     CDS             29920..30255
                     /locus_tag="ACG8VH_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109512.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="ACG8VH_000937"
     CDS             30402..30596
                     /locus_tag="ACG8VH_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109513.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="ACG8VH_000938"
     CDS             30628..33000
                     /locus_tag="ACG8VH_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9109514.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="ACG8VH_000939"
     CDS             33061..34521
                     /locus_tag="ACG8VH_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9109515.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="ACG8VH_000940"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="ACG8VH_000940"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9109516.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36752..36901
                     /locus_tag="ACG8VH_000941"
     CDS             36752..36901
                     /locus_tag="ACG8VH_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109517.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36975..37568
                     /locus_tag="ACG8VH_000942"
     CDS             36975..37568
                     /locus_tag="ACG8VH_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP9109518.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="ACG8VH_000943"
     CDS             37584..38483
                     /locus_tag="ACG8VH_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP9109519.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8VH_000944"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8VH_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9109520.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="ACG8VH_000945"
     CDS             39337..39597
                     /locus_tag="ACG8VH_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109521.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="ACG8VH_000946"
     CDS             40038..40244
                     /locus_tag="ACG8VH_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP9109522.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="ACG8VH_000947"
     CDS             40244..40495
                     /locus_tag="ACG8VH_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109523.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="ACG8VH_000948"
     CDS             40510..40947
                     /locus_tag="ACG8VH_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109524.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="ACG8VH_000949"
     CDS             40940..41647
                     /locus_tag="ACG8VH_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109525.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41812..42012
                     /locus_tag="ACG8VH_000950"
     CDS             41812..42012
                     /locus_tag="ACG8VH_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109526.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42028..42207
                     /locus_tag="ACG8VH_000951"
     CDS             42028..42207
                     /locus_tag="ACG8VH_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109527.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT        15576 a         6716 c         8659 g        11340 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat tttttatatt cacgaagata aggtcaaaga tcacgtgaaa
    26461 gacgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 accttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacttcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ttctaaagct acccgaaaag atgggtattt
    34621 tgagatccaa gatcgattgt ttacaggaga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgagtta gattcgcccg atatgtacga tgaaaaatgg aaacaatggg cattagaaca
    34741 tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga aaaagcagtt
    34801 tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga ctgatagcga
    34861 tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct atatgattta
    35161 tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag gaaaagccca
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa cccaagcgac
    35281 acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa aacagccagg
    35341 tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc ttttttcttt
    35401 atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag atactttgga
    35461 agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg atacccctta
    35521 tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca agcatttttt
    35581 aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg tgaataataa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggctat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaagtata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct
    35821 tcagttaaag gcaatcgaaa aggtgccgct agatctaggt tggaagaagt tatttggaaa
    35881 agagagcgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtggatctac aagttcttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc c
//
LOCUS       DAZKCC010000017        41516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000017 DAZKCC010000000
VERSION     DAZKCC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.017"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACG8VH_000952"
     CDS             214..498
                     /locus_tag="ACG8VH_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP9109528.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="ACG8VH_000953"
     CDS             complement(779..1198)
                     /locus_tag="ACG8VH_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP9109529.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="ACG8VH_000954"
     CDS             complement(1200..1784)
                     /locus_tag="ACG8VH_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP9109530.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACG8VH_000955"
     CDS             complement(1831..3306)
                     /locus_tag="ACG8VH_000955"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP9109531.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACG8VH_000956"
     CDS             3487..3798
                     /locus_tag="ACG8VH_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109532.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACG8VH_000957"
     CDS             complement(3913..6249)
                     /locus_tag="ACG8VH_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9109533.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACG8VH_000958"
     CDS             6391..7059
                     /locus_tag="ACG8VH_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9109534.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACG8VH_000959"
     CDS             7062..8105
                     /locus_tag="ACG8VH_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9109535.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACG8VH_000960"
     CDS             complement(8342..9223)
                     /locus_tag="ACG8VH_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9109536.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACG8VH_000961"
     CDS             9382..10110
                     /locus_tag="ACG8VH_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9109537.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACG8VH_000962"
     CDS             10266..10583
                     /locus_tag="ACG8VH_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9109538.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACG8VH_000963"
     CDS             10576..11184
                     /locus_tag="ACG8VH_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP9109539.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACG8VH_000964"
     CDS             11239..12030
                     /locus_tag="ACG8VH_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP9109540.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACG8VH_000965"
     CDS             12168..12656
                     /locus_tag="ACG8VH_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP9109541.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACG8VH_000966"
     CDS             12707..13579
                     /locus_tag="ACG8VH_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9109542.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACG8VH_000967"
     CDS             13755..14117
                     /locus_tag="ACG8VH_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9109543.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACG8VH_000968"
     CDS             14126..14878
                     /locus_tag="ACG8VH_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109544.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACG8VH_000969"
     CDS             14892..16694
                     /locus_tag="ACG8VH_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109545.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACG8VH_000970"
     CDS             16971..18011
                     /locus_tag="ACG8VH_000970"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9109546.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ACG8VH_000971"
     CDS             complement(18063..20207)
                     /locus_tag="ACG8VH_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9109547.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="ACG8VH_000972"
     CDS             complement(20564..21937)
                     /locus_tag="ACG8VH_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9109548.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACG8VH_000973"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACG8VH_000973"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9109549.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACG8VH_000974"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACG8VH_000974"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9109550.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="ACG8VH_000975"
     CDS             complement(23385..24398)
                     /locus_tag="ACG8VH_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9109551.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="ACG8VH_000976"
     CDS             complement(24479..25078)
                     /locus_tag="ACG8VH_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9109552.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="ACG8VH_000977"
     CDS             25264..25965
                     /locus_tag="ACG8VH_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109553.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="ACG8VH_000978"
     CDS             25975..28686
                     /locus_tag="ACG8VH_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP9109554.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="ACG8VH_000979"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="ACG8VH_000979"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9109555.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="ACG8VH_000980"
     CDS             complement(30825..32006)
                     /locus_tag="ACG8VH_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9109556.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="ACG8VH_000981"
     CDS             32258..33781
                     /locus_tag="ACG8VH_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9109557.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="ACG8VH_000982"
     CDS             complement(33850..34578)
                     /locus_tag="ACG8VH_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9109558.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="ACG8VH_000983"
     CDS             complement(34591..35871)
                     /locus_tag="ACG8VH_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9109559.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="ACG8VH_000984"
     CDS             complement(36012..36614)
                     /locus_tag="ACG8VH_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9109560.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="ACG8VH_000985"
     CDS             36859..37065
                     /locus_tag="ACG8VH_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109561.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="ACG8VH_000986"
     CDS             37377..38096
                     /locus_tag="ACG8VH_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9109562.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="ACG8VH_000987"
     CDS             complement(38177..38860)
                     /locus_tag="ACG8VH_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP9109563.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="ACG8VH_000988"
     CDS             39078..41264
                     /locus_tag="ACG8VH_000988"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9109564.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13480 a         7352 c         8323 g        12361 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag tgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataaa ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    41281 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    41341 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag
    41401 ggaggtgtga caagaatttt gtcacacctc ccttatttat tttgaaaatg agaatactat
    41461 tatcaaaata gagaatatat agaaaaattc tcattattca aacgtaaata atcagt
//
LOCUS       DAZKCC010000018        36944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000018 DAZKCC010000000
VERSION     DAZKCC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.018"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..435
                     /locus_tag="ACG8VH_000989"
     CDS             250..435
                     /locus_tag="ACG8VH_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109565.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            448..696
                     /locus_tag="ACG8VH_000990"
     CDS             448..696
                     /locus_tag="ACG8VH_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109566.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            727..897
                     /locus_tag="ACG8VH_000991"
     CDS             727..897
                     /locus_tag="ACG8VH_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109567.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(999..1466)
                     /locus_tag="ACG8VH_000992"
     CDS             complement(999..1466)
                     /locus_tag="ACG8VH_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109568.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            1625..1942
                     /locus_tag="ACG8VH_000993"
     CDS             1625..1942
                     /locus_tag="ACG8VH_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109569.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            1875..2354
                     /locus_tag="ACG8VH_000994"
     CDS             1875..2354
                     /locus_tag="ACG8VH_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFP9109570.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            2371..2547
                     /locus_tag="ACG8VH_000995"
     CDS             2371..2547
                     /locus_tag="ACG8VH_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109571.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            2804..3235
                     /locus_tag="ACG8VH_000996"
     CDS             2804..3235
                     /locus_tag="ACG8VH_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109572.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            3241..3558
                     /locus_tag="ACG8VH_000997"
     CDS             3241..3558
                     /locus_tag="ACG8VH_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9109573.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            3677..4363
                     /locus_tag="ACG8VH_000998"
     CDS             3677..4363
                     /locus_tag="ACG8VH_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9109574.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            4338..5687
                     /locus_tag="ACG8VH_000999"
     CDS             4338..5687
                     /locus_tag="ACG8VH_000999"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9109575.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            5690..6151
                     /locus_tag="ACG8VH_001000"
     CDS             5690..6151
                     /locus_tag="ACG8VH_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFP9109576.1"
                     /translation="MGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIARNKNN
                     EVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNWDKRR
                     NKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            6153..6653
                     /locus_tag="ACG8VH_001001"
     CDS             6153..6653
                     /locus_tag="ACG8VH_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFP9109577.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            6668..8980
                     /locus_tag="ACG8VH_001002"
     CDS             6668..8980
                     /locus_tag="ACG8VH_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFP9109578.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            9227..9544
                     /locus_tag="ACG8VH_001003"
     CDS             9227..9544
                     /locus_tag="ACG8VH_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFP9109579.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            9541..9702
                     /locus_tag="ACG8VH_001004"
     CDS             9541..9702
                     /locus_tag="ACG8VH_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9109580.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            9705..10004
                     /locus_tag="ACG8VH_001005"
     CDS             9705..10004
                     /locus_tag="ACG8VH_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109581.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            10004..10318
                     /locus_tag="ACG8VH_001006"
     CDS             10004..10318
                     /locus_tag="ACG8VH_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP9109582.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            10311..10841
                     /locus_tag="ACG8VH_001007"
     CDS             10311..10841
                     /locus_tag="ACG8VH_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP9109583.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            10838..11107
                     /locus_tag="ACG8VH_001008"
     CDS             10838..11107
                     /locus_tag="ACG8VH_001008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109584.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            11037..11240
                     /locus_tag="ACG8VH_001009"
     CDS             11037..11240
                     /locus_tag="ACG8VH_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFP9109585.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            11237..11524
                     /locus_tag="ACG8VH_001010"
     CDS             11237..11524
                     /locus_tag="ACG8VH_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109586.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(11557..11808)
                     /locus_tag="ACG8VH_001011"
     CDS             complement(11557..11808)
                     /locus_tag="ACG8VH_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109587.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            12011..12424
                     /locus_tag="ACG8VH_001012"
     CDS             12011..12424
                     /locus_tag="ACG8VH_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9109588.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            13044..13208
                     /locus_tag="ACG8VH_001013"
     CDS             13044..13208
                     /locus_tag="ACG8VH_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109589.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            13205..13435
                     /locus_tag="ACG8VH_001014"
     CDS             13205..13435
                     /locus_tag="ACG8VH_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109590.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            13620..14165
                     /locus_tag="ACG8VH_001015"
     CDS             13620..14165
                     /locus_tag="ACG8VH_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9109591.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            14262..14483
                     /locus_tag="ACG8VH_001016"
     CDS             14262..14483
                     /locus_tag="ACG8VH_001016"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9109592.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(14640..14804)
                     /locus_tag="ACG8VH_001017"
     CDS             complement(14640..14804)
                     /locus_tag="ACG8VH_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFP9109593.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            14906..15427
                     /locus_tag="ACG8VH_001018"
     CDS             14906..15427
                     /locus_tag="ACG8VH_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP9109594.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            15414..15854
                     /locus_tag="ACG8VH_001019"
                     /pseudo
     CDS             15414..15854
                     /locus_tag="ACG8VH_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(15894..16052)
                     /locus_tag="ACG8VH_001020"
     CDS             complement(15894..16052)
                     /locus_tag="ACG8VH_001020"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFP9109595.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            16145..16906
                     /locus_tag="ACG8VH_001021"
     CDS             16145..16906
                     /locus_tag="ACG8VH_001021"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9109596.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            17093..18445
                     /locus_tag="ACG8VH_001022"
                     /pseudo
     CDS             17093..18445
                     /locus_tag="ACG8VH_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            18461..19681
                     /locus_tag="ACG8VH_001023"
     CDS             18461..19681
                     /locus_tag="ACG8VH_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9109597.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            19716..20393
                     /locus_tag="ACG8VH_001024"
     CDS             19716..20393
                     /locus_tag="ACG8VH_001024"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9109598.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            20390..21583
                     /locus_tag="ACG8VH_001025"
     CDS             20390..21583
                     /locus_tag="ACG8VH_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9109599.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            21598..21924
                     /locus_tag="ACG8VH_001026"
     CDS             21598..21924
                     /locus_tag="ACG8VH_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9109600.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            21928..22263
                     /locus_tag="ACG8VH_001027"
     CDS             21928..22263
                     /locus_tag="ACG8VH_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9109601.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            22260..22655
                     /locus_tag="ACG8VH_001028"
     CDS             22260..22655
                     /locus_tag="ACG8VH_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9109602.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            22652..23023
                     /locus_tag="ACG8VH_001029"
     CDS             22652..23023
                     /locus_tag="ACG8VH_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109603.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            23039..23599
                     /locus_tag="ACG8VH_001030"
     CDS             23039..23599
                     /locus_tag="ACG8VH_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP9109604.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            23724..24035
                     /locus_tag="ACG8VH_001031"
     CDS             23724..24035
                     /locus_tag="ACG8VH_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109605.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            24059..24250
                     /locus_tag="ACG8VH_001032"
     CDS             24059..24250
                     /locus_tag="ACG8VH_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109606.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            24250..28098
                     /locus_tag="ACG8VH_001033"
     CDS             24250..28098
                     /locus_tag="ACG8VH_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP9109607.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            28099..28971
                     /locus_tag="ACG8VH_001034"
     CDS             28099..28971
                     /locus_tag="ACG8VH_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFP9109608.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            28968..30017
                     /locus_tag="ACG8VH_001035"
     CDS             28968..30017
                     /locus_tag="ACG8VH_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109609.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            30021..30845
                     /locus_tag="ACG8VH_001036"
     CDS             30021..30845
                     /locus_tag="ACG8VH_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109610.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            30863..32923
                     /locus_tag="ACG8VH_001037"
     CDS             30863..32923
                     /locus_tag="ACG8VH_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP9109611.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            32940..33089
                     /locus_tag="ACG8VH_001038"
     CDS             32940..33089
                     /locus_tag="ACG8VH_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109612.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            33086..33532
                     /locus_tag="ACG8VH_001039"
     CDS             33086..33532
                     /locus_tag="ACG8VH_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109613.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            33534..33671
                     /locus_tag="ACG8VH_001040"
     CDS             33534..33671
                     /locus_tag="ACG8VH_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9109614.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            33706..33948
                     /locus_tag="ACG8VH_001041"
     CDS             33706..33948
                     /locus_tag="ACG8VH_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFP9109615.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            33964..34161
                     /locus_tag="ACG8VH_001042"
     CDS             33964..34161
                     /locus_tag="ACG8VH_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9109616.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            34172..35188
                     /locus_tag="ACG8VH_001043"
     CDS             34172..35188
                     /locus_tag="ACG8VH_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9109617.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            35303..36511
                     /locus_tag="ACG8VH_001044"
     CDS             35303..36511
                     /locus_tag="ACG8VH_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109618.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            36495..36665
                     /locus_tag="ACG8VH_001045"
     CDS             36495..36665
                     /locus_tag="ACG8VH_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109619.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT        13020 a         6095 c         8074 g         9755 t
ORIGIN      
        1 ttggttgaca attagctttt aagctagtat atttaattcg taagctaaat tgttagctaa
       61 ataagagcaa caaaaaactc tactaattta aaacattctc tcggtcgcca aacttagaaa
      121 tgttatttta gaggcttttt ataagtctta tttaactatg tattcataat agcttaaaag
      181 ctaataggtg tcaacgattt agctaatttt ttagcttaca aattatttgt ttagaaagga
      241 gctattttta tgtctgagaa tttagactta aaaattcgag cggagatgag aaaaagaaga
      301 atgactttca aagaactagc tgcgcttatc ggtatttcag gagcttattt atcagatatt
      361 ctaaacggca atcgtgatgg aaagaaagca caacagcata tcgaaacagt gaaaaaaata
      421 ttggacatcc gatagggggg gtaggcgatg acaaaactaa aaaaacaaga ttttgtaaaa
      481 aaatataatt attctccatc tacttatcaa cgtcgaatgt cggaactaaa aaatacagca
      541 attttctcag cggcgtatga acgggtcaca ggacaagaag tttggatcaa tacagaatta
      601 tacgataaat ttttgtcttt caaatcctat aacaggttac gcacaagaaa ggtaacacct
      661 aaagaattta tcgagaagca tttagttgat ttataaaaaa cagaacattt tgagaggtga
      721 aggttaatgg gtaaattcaa cagagcatta gtattcagcg caccgctaat cgtctatgct
      781 ttagggcttt ggggaagtag acaagcgttg ataggaacga ttgtgtacat ggtttggatt
      841 tttatagggc tggatgaagc tgagtacaaa acaaaaaagc cagtcgggag ggactgacta
      901 atgagtaagg acgatattgt atttctgtta attatgtttt gtgggattgg atcaataata
      961 attaatttgt atttgattat taaagaaaaa tttttcaatt aaaatgagtc ttttagcttt
     1021 aacttaaatt ttttttgata attagctgtt cttatgacca ttacaagttt ttcaccaggt
     1081 aatatatctt tcatatctaa aacttctgaa aaagcgactg agtagccagg agagattgct
     1141 tgtgccaacg gtataggaga taaaaggcta cttttatgaa tgcttcccat atcaaaacca
     1201 ttggcgtgat aatgtgaaca attaattttc ttaccgttgt aaagatacgt acattcgata
     1261 attgagtttg gcatttttga tgggtttgca attacgccag taagaatcat tcgattactt
     1321 ctatcatgct taactaaaaa ataggagagc gtaatttttc ttttatcttt aaataagtta
     1381 tagagggata aaaaaaatcc tgaaatagaa atgattaaag taatagcgac ttggtaatcg
     1441 ctcaaaattt ttatcaagta attcaccacc tttttactca aattatatca aaaaggagag
     1501 aagaaataat gcaagaatta gtaattttga aaaataaaaa aagacacgaa cgccggcaag
     1561 catgatcgtg tcagggaaat aaactatctt tctatatttt accacagaaa ggaatgaacc
     1621 gtaaatgcta attgcaacgg atacactaga ccgcatcttt ttaaaagacg aatacaaact
     1681 gcgcaatata gatgcgtcag gaattttagt atttgatctt tatgacaatg gaaaaattgg
     1741 tatctatcaa gcaagtgata ttgaagaaac aaaccttgct ttcgaacaaa ttgatgattc
     1801 tgtggaattg gatttagatg aggcaatcct agcttttgaa caaattgcaa aattattaaa
     1861 ggaggcacaa aagaatggca actctttacc aactcagcga gtcatatatc aaagtcctgg
     1921 aactggcaga acaattggat gaagaaattc ttcgcgatac tctcgattcg attaatgaag
     1981 cgatcgagta taaggcagaa aacttagcaa aaatagttaa agaagtagaa gggaaagctg
     2041 agttaatcga tagtgaaatt aaacgtctgc aggaacgtaa gacatcgctt ttgaacaatg
     2101 ctaagagtat caagcactat ttacaagagg aaatggaaaa gactggtaaa acgaagatta
     2161 aaggtgaatt attcaacatt ggaattcaaa ataatccagt atcggtcaat gtaatcaatg
     2221 agaacttaat tccaaaagga ttctttaccc ctgtgcctcc caaattggat aaaaagcaat
     2281 tgaaggagga actgaagcac ggagatattc ctggtgctga actcgtccaa acaaaaggtt
     2341 tgaggataag atgatgaagg aggagaaata atggaacacg aaaacgtaaa agacgctttg
     2401 aaagctgcta tcgaaatcgc ggaagctaaa ggaataaagg ttgacggcaa accagcaact
     2461 gtccatgaca ttcaaaattt gacgaaagaa catctttatt ttatcgccga cttgcttggt
     2521 ttgtcggaat tgtatttaga caaataaccc cacacggaag gagtggctag ggtggttgta
     2581 ttacaagtaa tagtctgcgt aacattcatt tggtcgtgca taccggtttg tatatatatc
     2641 atgaagaagc ttcgccctga gtcttacaaa ttatacgcaa tttacagcat aacagtagct
     2701 cttgcattta cattgtttgc aatttctagg cttccgtttt aatcgtggga atgaattttt
     2761 aatgaactaa attatctata aagcaaaaaa aggagaaaca acattgaaaa acaaactagc
     2821 aaaaacaaca gcaatcatcg gactagcact aggtagcggc gttatcggct acgcagcaag
     2881 caatgcgttt caagatttgg acacgatcaa agcaaatttc aacacagtcc tacaatacgg
     2941 tcaaacgaaa tcgcaacgtg tgtcagaact tgaatcacag ctatccaaca acactcgcac
     3001 acaggagcag ctgaaagccg aaattgagca aatcaaatcg gacaagcaga aggaaattga
     3061 agccaagcaa cgagaagtcg aacaaaagca acaggagatc gctacaaagc aacaggaagc
     3121 cgatagcttg cgccaacaac tatccacagt gcaaaacgac aaggagcagt tagaacagcg
     3181 cgtgagcgag ttacggcagt atacggatca aaaggtaggg gagttgggga aatgaaagag
     3241 ttgactattt atgacccaaa tatttcatgg gctaaacaca caattaaagt ttcttttatg
     3301 atttggggtt acaaaggata tgttacttac aaagtgggcg gtaacactaa gggtttatca
     3361 ttaatagcta ttgattcaga tgatctatat gatgctaatt tcgaggataa cccagttaat
     3421 ttccgtgacc ttgatgaaga ttggttctct atggaactta ctaacgataa aggcgacagt
     3481 acacttgtag aagatgaatt cgacagacta ggtgattata ttgtcggtgt tgaaattatt
     3541 gcgcacgaac cagagtagga gagtgaatac aaaatgatta aaagaatcac tatatcagtt
     3601 gaagaagata gtggatcaac aatgcagcat accgacaatc gaaaaacttt tttaaccaat
     3661 agaaaggaga attttgatgc agattaagaa ggcgacggat atcgaacaaa ccaagggaac
     3721 atatttgatc tacggtgctc caggaaaagg taaaacttca acggctaaat attttccagg
     3781 gaaaacactg atccttgata ttgatcggac ttcaaaagtc cttagaggtg agaaaaatgt
     3841 cgatatcgta tatatcgaca acgaagatac ctggaacgac tggggcaaca cgttggctga
     3901 tttaactacc aattatgtag gtgtttatga caacgtggtt gtcgataatg tcagcgaatt
     3961 ggaacggtgt attctgtcca gtttaggcgc agaaggaaaa aacaacggcg ttccttcgca
     4021 aggcgattat caatatatgc aatttagaat ggtgaacagc ctacgctata tgaaaaattt
     4081 agacagcaac cttatctgga ccgcatggga agaaatcgat ttgtggacgg attcggatgg
     4141 atcttcttac aacatcgctt tgccgcagat caatcgaaag attcgaaaca atattttggg
     4201 attatgtgac gttgttggtc gattgatggt aaaggaagac gatgaacgcg gatttatttt
     4261 acaagccaca gattcaacct atgccaaaaa tcaattggat aatagatctg gctgtaagca
     4321 aagtgagctg attttagatg gaattgtacg actaccagaa tgatttagtc gatcgtgcaa
     4381 gacaagccta tgttgatggt tacaaggcac catgtatcgt atcaccgtgc ggttctggaa
     4441 aatcagtcat ggttgcagag atcgctaaac gaaccacacg aaaaggcaac agagtgttgt
     4501 tcttggtcca tcgtcgtgaa ttgattgatc agatcaaaga tacgtttcaa aagatgggcg
     4561 tagataatca gcttgtaaat ttcggcatgg ttcaaacagt cgtacgacat cttaaaacca
     4621 tcaagaaacc acaactgatc atcactgacg aaaaccatca cgggttagca gcgtcctaca
     4681 gaaaaatcta tgagtattat tcggacgttc cgcgattagg ttttaccgcg acacccatcc
     4741 ggttaaacgg tagtgggttg ggggatgtga acgacttgct aattgaagga gtatcggcta
     4801 aatggttgat tgagaatcat agattagctc cttatgaata ttatgcacct aaattgatcg
     4861 atacagccga attaaagaaa gcttcgactg gagattttac aaagaaatca atggataagg
     4921 cagtgaagaa tactatttat ggcgacgtgt tgaaacatta taaaacgtta gcagaaggtg
     4981 aacaggcaat agcatactgt cacagtatcg atgcaagtaa acacactgct gaaatattca
     5041 acgatgctgg ttataaagca acacatatcg atgcaaaaac tccaaaagat gaacgtgcag
     5101 atattatcga agcgttcaga aatcatgaaa ttaaaatcct atgcaatgtt gatttgatcg
     5161 gagaagggtt tgatgttcct gattgttcga ctgtaattat gttgagacct actcaatcgt
     5221 tatcattgta cattcagcaa tcgatgaggg ggatgcgtta ccgaccagat aaaacgtcaa
     5281 tcatcattga ccatgtgggt aatgtgaacc gttttggatt gcctgatatg gatcgtgaat
     5341 ggagtttaga tacaaaaaag aaaacaaaaa ctgatagtga tttatctatc gttcaatgcg
     5401 cgttttgttt tggtgcttac gaacggccaa aaggcgataa tatttgtccg tattgcggag
     5461 agttgcagcc gattgaagaa cgaaagtcag agttagaaat cgatgaatcg gctgaattaa
     5521 tgaaagtcgg agaaacgaaa attactttga attttgagaa caataaatat tacaacatga
     5581 cggaagatga agcagaaagt atagaggact tatatgccat agcaagagct aaaggattta
     5641 aacctggttg ggcttatatg gctgctaaga ggaaggggtg gttgtaggaa tgggcggtat
     5701 ttttggtaaa aaagcagaag atgttcaagg ttcaataata aatggcgtaa caataatagg
     5761 tgatactgga aaaagagata gttcaggcaa tcaaattgta atcgctagaa ataaaaataa
     5821 cgaagttttt gaatcttctc tatctaatat aaaaagaggt gttgttaacg ggacaggcga
     5881 agagagtggc tacgttaatt taaaaaaagc taatgaaaaa taccatgttg aaggaactta
     5941 cgttaaggct ttgtctgtcg gtggaacaaa aggcgttaat tgggataaac gaagaaataa
     6001 atggaaagca cgattatatt tcaaaggaga atatgtctta caaaaggatt tcaacaccaa
     6061 agaagaagca atccaagcac gacttgcagc agaagaaaaa tatttcaaac ctatcttaga
     6121 aaaatatgaa aataaaaagg agaatgatta acatgacagg atttaactta gacttttcaa
     6181 aagcgcaaca agggaacgaa atcaaagacg gtacgtacga agtggtcgta aacaaagctg
     6241 tagaaaatgc aactaaatca ggcgcagaat ttatcgatat cgatttgatc gttcgtaacg
     6301 atgtggatca accgttccaa aataaacaca ttttcgcaaa aatctggaaa gccaaagcta
     6361 ccggcaaata taacgaaggt atgatcatgg caattgcaca agcgctacag ttggaagacg
     6421 gaaaatcata taacgggttt gatgaattgc tggcggactt tgttttgaaa accgcctcag
     6481 tacgcgtgaa aactgaagag tcaaatggat ataaaaacgt caatgtgaag tcttgggata
     6541 aaacgaacac acggggcgta atgaatcacc aattcaaaaa tggtgatgaa ccatccttcg
     6601 gaccggaacg ttcaagagcg acaacggtta gaaatgacaa tcttccattt taattgaggt
     6661 gtaatcaatg tatgaaaaaa ttccaaaaga gcttaaaaat ctcaaacaat ggtgtgttta
     6721 taagctagtt tgggatgaaa agcggcagaa gcacacgaag atcccttata acgccaacaa
     6781 cgggcataaa gccaaatcca atgacgagag tacctggtct gattttcaga ccgctctcgc
     6841 tgctattgat cactacggaa tgagtggact ggggttcttt ttcaaaccac cttatttcgg
     6901 cattgatatc gataatgctg aaggtgaagt tgagcgattt aaatcaggtg atatagaaga
     6961 aaatattatc tatgaattta tcgaaagcat gagatcctat gctgagtatt ctcaatcagg
     7021 tactggcatc cacattattg ctcgcggaga attgcccggc ggaagacgca ggaaaggtga
     7081 cgtagaaatg taccagaacg gccgattctt cgttatgact ggaaatgccg catcaaaata
     7141 tttagaaata actgagccga atccaaagga tatcaaacgc ctatatgatc gctacgttgg
     7201 agatgaaaaa atcatccaat tcaaagaaga aaatccgctg atgaataccg ttgatttacc
     7261 ggttgaagaa attatcaaac gtgctgaatc gtcttcccaa ggggcaagat tcaaagtctt
     7321 tatgaacggc ggttgggaag ctttttattc ctcgcaatcc gaagcggata tggcgttcgc
     7381 caacgacttg gctttttgga ccggtagaga ctttgaaaaa atggatgaga ttttcagagc
     7441 gtctgcaatg atccgtagca aatatgacca aaaaagggga tcggcaactt acggagaatc
     7501 tctactaaat aaggcaattg ctgatacaaa ttccgtctac aaccccaaac ggaaatctga
     7561 ttttaagatt tttatcaaag accaggaaca gccaaaagag gaaaaatact attcgtacga
     7621 cgacaccggc aacgcggatc ggtttactga tatctacgga acgttagtca aatattcgta
     7681 cattgataaa tcttggtatt actacgatgg gaaagtttgg ctacaggaca ataccggcga
     7741 agttcggaaa atgatcgata cgacagtaga catcatggga aaagaaccgt taacgatccc
     7801 tgaaggcgca gacgatgaaa ccaaagaagc tttgatgaaa gcgaaagaaa aacacgtcaa
     7861 gcgttctcgc agcaatgccg gaaaaaacgc tatgatggac gaattgaaac atcgactatc
     7921 cgtattgccg gaagagttcg acaaagataa aacgctgttc aacacacaaa gcggctatct
     7981 gtctttgcat gacggtcttc tacacgagca tgagatagac aaaatgttta cacgtgtttc
     8041 aaatgtcgag tacacagaat cggtcgattg tcctatgtgg gaagaattta ttaatcagat
     8101 ttttgacaat gatcaagaat taatcagata tatccagaaa tgtgtcggat attccctgac
     8161 cggatctacc agagagcaat gtatgttcat cctctacggt catgggtcaa atggaaagtc
     8221 ggtatttttg gaaattattt ctgaattgat gggaaactat gcgatgacta tgcaggcaca
     8281 aacgatcatg gtcaagcaaa gccaatcatc agcaaacagt gatattgcac gattgaaagg
     8341 tgcgcggttg gtcacttcgt cagaaccgaa tgaaggcgtc cgactggacg aaggtctggt
     8401 caaacaattg actggtggtg ataaagtcac ggctcggcac ctttatggaa aagaattcga
     8461 gttcgaacct gaattcaagt tgtggctggc aacgaaccac aagcccatca tccgtgggac
     8521 cgatgacggc atctggcgac ggttaaattt aattccattc actgtccaaa tcccggatca
     8581 caaaaaggat aaaaatctga aatttaaatt gcagacggag ttgcaaggga tccttaaatg
     8641 ggcaatcgat ggttgcctgt tgtggcagag agagggcttg gagaaaccaa agtcggttgt
     8701 tgcagcgagt caggattatc gaaacgaaat ggatcaaata ggtacattta tcgaaacgtg
     8761 ttgcgaaaca ggtccaggac taaaaatatc aggcggagaa ttatacaaag tttatcgtga
     8821 atgggcatca gataatggcg agcatacttt cactaacacg aagtttggca gagagatttc
     8881 aaagaaatat agcaaagaaa aagctggtgg atttatggtt tataaaggaa tcacattaaa
     8941 accaagaaaa tacgataacg ttagagaact ttttaaataa atctggaggg ttctggaggg
     9001 ttgagaacga aaccctccaa cccttagaaa cgttgatata tcagtattta tattatattt
     9061 ttctttcttt ctggagggtt ttaaaaaaat aataaagtat taaagtaaaa aataaaaagt
     9121 aaattatata aaaaagaata ggtttttggg tctgaccctc caaactctcc accaaaatct
     9181 tcctaaatgc tttgatacca acgattaaac agtctggagg gttacattga aatcagaaca
     9241 tcaaatacaa gacgagatca gaattgcctt atcaaaacat ggttgcacga tttttcgatc
     9301 caatgcggga caagtgacaa cgatcgaagg gcggcaattt tacgggatgc caaaagggtt
     9361 cccagatcta tgtggacatc gtaattctga tgggaaagca atttatatag aagtaaaaaa
     9421 tgaacgcggt aagttgcgtg aagatcaaaa aaggtttgct gaatttttaa acggtcaacc
     9481 tgtactatac ggtgttgcac gatcagtgga agaagcattg aaaattgtgg aggaatcatc
     9541 atgacaacag aagaagtgat tcaaatgcgt attcgaagcc ttcagcgtga gattgacgat
     9601 ctggaacgga caaaggcagt gatggtcaat gaaacggcga ggaaggcaat cgatttgcac
     9661 atagagaatt taagaaggga aatccatcga ttggaggaat gagcgtggat aagaaagcag
     9721 caatgaaacg aatcatcgaa ctgacacatt ctgagaattg gcaagaagac aaagaaatag
     9781 ttgcagaagt ccaaaagctc ggtaaatcaa tgtggagtga aaaaaccaaa cggagaacac
     9841 cgagaaaaat tgcaatctgg catggtgacc gaattctagt aacaggtact gctgaacagt
     9901 tatctgaaat tactggatta agcaaaaaca ttatctggga tagagctaaa cgcgagaacg
     9961 ttgattcgaa aggtcgtcaa tttaaacatt gggagaagaa ataatggagg aactaatcac
    10021 aaaagtagag cagtgggcaa aagataaggg attggatcaa gctgattcca gcaagcaaat
    10081 gttaaaaacg atcgaagaga ttggggaagt tgccgcttct ctagctagaa aagatgaata
    10141 tggtttaaga gacggaattg gagacgtagt agtaaccttg attattttag ctatgcaaaa
    10201 tgatatggat ttgtacgagt gtctgaacca agcatacaac gaaatcaaag gacgcacagg
    10261 aaaaatggta gatggtgtat tcgtgaagtc gagtgatttg gaggacagcg atgaataaaa
    10321 aagtattaat tgataaacaa gcattgattg atgaattaat gaaaatacct ggtgtgggat
    10381 ctaatagtga cgctttagaa acgattaaac gtttcccatc gtatgaaccg cagaagccag
    10441 ttgtgcctaa gtttgtggcg gaactactcg actattatcg tcagtcaaca gatgttgatt
    10501 tattagcttt attgataact ttccatgatt ggtattatcg caaaactaaa gacggtgagc
    10561 atgaagaagc aattgattgg ctagttgatc atcctgaaat ttatatgcgt gcatggctgt
    10621 ttggctacga gctcgagaaa gagccgttgt attgcgtaat aatcaatggt agatatcttg
    10681 tcaaagtatt cagcaataca aacgttgtca tattagttcc agcagatgaa ttttcacagt
    10741 acgttactca aaaatttcaa ttaactgaaa aacaaatcaa agcaattgat gaaagatact
    10801 ggccgtttgc tgtgccagtg gatgaggtgg tagaaggatg agcgacccat acattactaa
    10861 attagaaaag ttgtccgaag attttgagaa gaataaaaat aaactaacta aagataaact
    10921 tatagaactg gttaggttgg ttatcagagg agctattaca agagaaaagg cgcagcaaaa
    10981 catagaaatt tttgctcgta gtgtccgaga tggattagag aagaaagtta aacaaaatga
    11041 acaacagaca ccgcagaata acaaaactaa gaaaacggga actgaatgta ctaaagacaa
    11101 agtttgaaaa agaatatgga atttcagcag aagaaacata taaagtggca agtcagtgtg
    11161 ttgctgatgc gagcggcgct attcgtaagt ttggaatttc gatattaagt gatgatcgta
    11221 aatgggagga aaaagaatga aactaaaaga cggattttac tccagcagtc atggtatcgg
    11281 cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgtgagaaac caaaattcaa
    11341 agtcggtgac atggttcgct gtgaagcaga aggattcatc tatccatttt gtggatatgt
    11401 agaacatctc tataatcact cagcaatcat tcgtattgaa aacacgatgg aatgtgacaa
    11461 gtggttagcg aaaagcaaag agaatttagc agtagcgaga ttggtggata tggaggttat
    11521 ataaacagcg aaaaaatgta cttctaagag aaaaatttat tttttataaa aataggaaag
    11581 tatcattatg aaaaagacca cagagataaa taaacctacc acataaaaaa tgaatatttc
    11641 ttgcgattca atataggtat atttaacaat ttgtacaata aaaaatacta taggtaaaaa
    11701 agataacatg aggagcttat ttgttctaga gtgtttgaca aagtaacaaa atgcgacgat
    11761 accaatacaa acgggaatcc aaaataacag catgctccac atagtcactc aaccatctcc
    11821 tatacgcttt attatcaata gataatatca tttttcgtaa taaagataaa aaaataagta
    11881 aggcttctca ttaaaatata aaaaagacag ccgaccactg actgcctata taagagtatt
    11941 gaaataaaaa gctgctgata taataaattc cacaagttta ttatatcaca taaaggagcg
    12001 gtttgacttg atgcaattgt tacgagaggt agatttcaaa cagacaagat gtaatgcgag
    12061 agatgtgctg aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat
    12121 taagtcgccg attattacgg atatgccgaa ggcgccgaag cacggcaata aggcagaaga
    12181 cgcaatcatt cagatgatgg atatagaagc ggagagagac gcgattctag cagccttgat
    12241 ggctcttagt ctgattagtc gtcagatact ctactatagc ttttgtgtgc cagatagctt
    12301 ctcaaactac agaattagcc gtgaagtggg ttattcagaa agaagtatac aacggatgaa
    12361 gtcggaagct ctaatagagt ttgcagaagc atataaacac ggaagaataa ttgcttataa
    12421 ataatttggc ggctttttgg cggaatgatg gcggttttta gctatttacc agtgatatta
    12481 tggtagtgtc gaaagattag tgataggtct aagacaaaat aataataaaa ggaacatcgt
    12541 tttattattg tttcacaatt aagcttcgat agacagcaac ggaaatatta agaataagga
    12601 tgtgaatttt aactccttct aaattgttct tattatctat catccgttgc tgtctattgt
    12661 ttttttaatt attcacacga taactaaagg tgagcgaaga gaaatgattc cattaataat
    12721 ttcaattttt gcgctttgtc ttaatgtcta tatgattgga tttaaaaatg ggcaaaataa
    12781 aagatagtag taactaagaa agaaattttt atattgtcac tgtggcggaa agggtagacg
    12841 ctaagcatgt gtgctaggtc aatgcttcgg caactatgca atgttcgatt cattgccagt
    12901 gactttggca accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg
    12961 ttttgtgcat cgtgtaggtt gctattacat attagatcac tcgttgagtg gtctttttat
    13021 tttacataaa ggaggtaaca acgatgtata gaccacaata cttagaacag aagtatgaag
    13081 taatcactgt gcaaaacggt aacggtgaga tagtacgaaa gtatagaaga ccaataaaga
    13141 gcgatacata taaacgaaag gaaaacaatg aagttatttc ttttcgcaga aggagaaaag
    13201 ccaaatgaga aactactggt atgtatcgct aactaatgaa tatcctcgaa ccattgatga
    13261 ttgttcagtg cgtgttgtgc gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat
    13321 gctaagagaa gctacaccaa acgaagtgga taaatgcaag ctgatatatt gcggtcatgg
    13381 ctattggaaa gacgagtata tccaacaaaa cattgagagg tggatagata gatgagttac
    13441 ctcgaacatt tgaaacgttg ctacatgcat tctaagaata aacttcctga cagctacact
    13501 gtagatgatg ttgctataca tgtattgaag actgagagcc atagcagtcc tgatggtagc
    13561 agtaaggagc agacgctcgc gtggttcaag ttctttaagt ggattaagga ggaagagtaa
    13621 tgcacatgca cataggagaa aagatatata aattgagacg gcttaataac gaaacaatga
    13681 acgacttagt taaacaaact ggagtgtcaa aatcatggtt atctgatatt gagtctggaa
    13741 aaaagcaacg cgttgatttt aataagatat gtgcaatagc caaacattat aaagttagct
    13801 tagaatactt tcgggatgat tatttggata gtgatgaaca cgaggatatt attcctttga
    13861 caggcgaaac aagagttaaa tcagtgaaat cgcttgaaca aagcctaaag tatctgaata
    13921 acaaacaaga acctaacctt ctagagatca aactaaaaga tactgactca gtaccagagg
    13981 tttggtacaa gggtgagagg ttggatgaat tgcctaaagg attagtagat gtctcgtatc
    14041 attggaagac tgatgatttt actaatgatg atagaggagc gaacgacatc acgattcaat
    14101 acttttctag ctttaatgat aagtatccag ataagaaaac aatcggacac aagagagata
    14161 tgtaaatgaa agaagttaga cctagagacg aaatagacaa actatacaag accaaacgat
    14221 ggcgagacct aaggcaagta gtaatagcta gggacttcgg catgtgccaa gagtgcaagc
    14281 gtcgagggcg gaacacaagg ggcacgatca tccatcacat agtcgaggcg agggaagacc
    14341 tgtcactgtt ctggtccgta gataaccttg aatgtatctg tgtagcttgt cacaacagag
    14401 agcatccaga gagatcaggc gggaagaaga aaccaaaacc taaatcacat atcgttaaaa
    14461 tgtattcaac tcctgaaaga taagtttgca gcgaaatgaa ggtagccccc ctactctaaa
    14521 agattaaaga gtaaggcttg agaagaacgg tgctgtcctt ccttcgtaaa aagaccgctt
    14581 ttcaagtttt ttggagaaaa aggaaaaagc cgaccaattt aagccggctt tggacgaagc
    14641 tatttcttag tccatttgtt tcctttttga gaagtagggg gtaatcggtc gcctggatca
    14701 atttttactt cttgtccgcc ttggacattt ccaccacgag gacccacttc tttgtaggtt
    14761 ccttttggtt tgttgtcttc gccgggttta tagagttctc ccataggaat ccctccttaa
    14821 caaatttcgg cacagcactg ccgataacct aattataagg attgtgataa cgatttcaat
    14881 ctatcttttg aaagaaggtg atattatgcc gcaaccagcg aagagtgcaa aattacaatt
    14941 attaaacgga aacccaaata agaagaatac cgaagaactc cgcaagcgag cggccgcaga
    15001 agacaaatta aaaatggcta ctgacaaaat caaaccgccg ttatggctag attcgctagg
    15061 aaaggatacc tttgagttta tcgccgatga attgctgtct gtggatttaa tcagtaatcc
    15121 ggacgtccat acaatggctc tatactccaa ttggtattcg caatacgttt ctttagaaaa
    15181 acagcttcga aaactacaac gagagtacaa gttgaactat gcgcttgcga aaaaggaggc
    15241 agaggcgaga ggtgagccgt ttaatgaacc taatgaatta attggtaatc cgctctctcg
    15301 gcagatggat acagcgtctc ggaatctccg ttcttttggc gctgatttag gactatcacc
    15361 agcagccaga gctaagttag ctattaagat ggctgatgat ggtggtgatg acgatgacga
    15421 cttctaatat tttggatatg tcttacacag aacgtgtgga ctattggcaa agctatcttg
    15481 aggagcaagc ttcttggggt ggctttttaa aatgtccata tccggaattg ttaactactt
    15541 ggtatgcgga acgattaatc gatggaagca taccagccag caaagaaaat attcaagctg
    15601 ctaaacggca tatgcgtgat ttgcagcgcc aaggaacaga tgattttcct tggatctttg
    15661 acgaagaaaa aggtcaccgg cctattagat atatcgaaaa aaaatgtaaa ccaactgaag
    15721 gcgactttgg ttcgtttgtt ttgcaacctt ggcagcattt cataattgga tccatgtacg
    15781 gatgggtaca tcgtgataca ggagagcgtc gcttccgcga ggctcttatt tttgttggac
    15841 gtaaaaacgg taagttttgc cgtttaaaaa tcgggcaaaa tcggtgaaaa cctttatttt
    15901 tggctttctt ttagtaataa ttcgatagct ttatcaagca atttagacat aggaaccata
    15961 gtttcttccg acattttttt taatccttcg tataaatctt tatcaattgc gttagaaatt
    16021 ctaattctgt tttttagtcc gtatttgtcc atgtgtgaca ctccttttta ttttactata
    16081 acatccgatt ccaccgcttg caactaccat caattgatgg tagtatataa aagaggtgag
    16141 tagattgaaa aaatacatta tttacaaaca cttaaataaa ataaatggca aaatttatat
    16201 cggtgtcaca aatgatattg gcagaaggtg gaggagcggt gggattgaat ataaaccacc
    16261 aaagaacgaa acccaacacc acagaagttt ttggaatgca atacaaaaat acgggtggga
    16321 aaattttgat catttgatta ttgaagaaga cttaacgatg aaagaagctt ttgagaaaga
    16381 aaagttttat attgaacttt atgactcaac taataagaaa aaaggttata acatcgcaaa
    16441 aggcggaaat ggcggaataa tatataaagt tcaccccaaa ggaatgctag ggaaaaaaca
    16501 atctaaagaa ttttcttcaa atcacagtaa atgggcaaag aaccataaga ataattgtat
    16561 gacaaacggt gatgttgtat ggggtgttac tcacgaacac ccaaaaggca tgttgggaaa
    16621 gcaccacagc aaagaatcaa ttatgaaaaa gaaagcatat agtggtgaaa atgctgtaac
    16681 ctcaaaacca attgtcgcaa ttgaacggga tggaactaaa agagaatttc acagcgctaa
    16741 gctttgcatg gcttactatt cgattagtac atgtgttttt taccgacttt taaaagatgg
    16801 cgccccttat gtgataaatc ctaaagcgaa ctacagaaat aaagaaaaaa tattagctat
    16861 cgaaggaatt atgttcaaac ataaagaaga taccgaggta agttagtaga ttgcgaaagg
    16921 ttactaacta ccgtaacgcg taggagttga ataaatataa tgctcccaag agtgtccgac
    16981 agcagataat ttgctgaaaa tgtacgctaa actgggccag aactgactga ccgatgaaaa
    17041 tgagggtgac ctccagagtg cgagataaaa agctcgcaga taataacaat cgaaaacatc
    17101 actaatttca ggcctttcca catacatggt cgcttatgat gatgaacaag gcgccaacgt
    17161 ttacgtattg gcaaatgctc gtgatcaagc aagcttgttg tttgataagg ccgcagaaat
    17221 ggtcaaacaa tcgccggcgc tctttaagaa atttggtaag cctaaacgat caagtattaa
    17281 ttatgctccc gccttttcta aaatggaacc acgcgcctca gatagccgga aattggatgg
    17341 gctaaacact cactttggta tttttgacga gatccacgag tttacgaatt acaatctgat
    17401 caacgttatc aagaaatcaa gaggaaccag aaaacagcct ctgatagttt atatcacaac
    17461 tgctggatat gtattagatg gtccgttgat gtcttatttt gagcaaggtg tggactgttt
    17521 ggaacatttg gaagatgaca tcgatgaacg gactttctat tatctggcaa aacttgacag
    17581 tgcggaagag gctgatgacc caagattatg gatcaaagcc aatccgaata tttgtctaat
    17641 gaattttgtt ggcatgctag atgactatgt taaggataaa aaagatccaa aagaatatgc
    17701 tgactggatt accaagcaat ttaacttgtt ttccgatatc gatgagctgt catttgtcga
    17761 tatgcctacc attaaacgaa acaataaaac catcgatatt gaaacgctca aaggtaagaa
    17821 gtgtgtcggt ggttttgact tgtccgaaac ggaagacttt accgcagccg ttttagaatt
    17881 tccgcttgaa acaggcgagg tattcatttt gcaacacaca tggatcccac aagctagatt
    17941 tgatcgagat aacaatcaag agcgtatcaa agcgtgggag aaggtgggag atctaacgat
    18001 tattcctggt gattacgtca attatgaata cgtcttaaat tggtttgtag aaaattcgaa
    18061 aatctatgac attgtaaaaa tcaattatga caaggccaag gcgctacgat taaataaaga
    18121 actagaaaat gcaggatttg aaaccaacga gattcggcaa gggtttctat cattaggtgg
    18181 gccaatgcaa aacttcaagg aaatgctatt ggacggtaag gtgattttca acaattccaa
    18241 gctttaccga tggtatctat ccaacgtcaa gctggtgatg gatcgcaact caaactggat
    18301 gccgtctaag cagtccaaga gtagaaaaat agatggtttt gcagcaagtt tgaacagcca
    18361 cgccgaagtg ttgaatatgt tggttaatcc tgtcggaacc gggaaagtaa cctattactc
    18421 gatttccgat ttaatgaata tgtaagaaag gtgtggagga atgagtattt tagatcgttt
    18481 gcgttctttt ggccgagcga agccgaaagc gagcaaacaa gagtattttt tgaatgaccc
    18541 gggattgata ccgtatttag tcggaaaaga tgaaatatca gaagggattt tttccgtaat
    18601 tagccgtgta tcgaacgttt ttgcgtctct ccctctcaaa atgatagatg tggagtttgg
    18661 ccaaccggac gactgtcctg catacaactt gttgagcgaa ggccctcgat attttacaaa
    18721 gtttgatttt ttccgggacg tggaagtttt gagaaactac caagggaatg cgtatgtgca
    18781 gattttccga aatatcaatg gagaagtagc agatatggcg ttagtaaaac ctggtgcttg
    18841 ccatccagtg attgatatgg atagcgggga gctttactac caagtaactg cgactgacaa
    18901 aggcagttac aagcaagtta tctatgtaca ttacatggaa atgcttcact ttaaacaacc
    18961 gaggtttggc ggcttggaag gtacagaccc cacaaaagta ttaacgaata ccctcggata
    19021 tgatcgagaa gtccgaaaaa tctctttaag tcagcttaaa ggaagtaatg aagggctaaa
    19081 agttaagttt gctagcaata tggatgaaga agctaaaaaa gctacagtta aaaacattgc
    19141 tgatttttat cgacaaaacg gtggactact tgtggaagaa aacggtgtag aaatcgaacg
    19201 tttacaacga gagctggtag acagcaagct tttagatact gataaagtat ctcgctccag
    19261 aatcgctatg gtctacaatg tgccggagca tttcatcggg aataaccagt cgagttactc
    19321 ctctcaggaa cagctcaata tggaattttt gacgtacaat ctagtaccga ccgttaatca
    19381 atatgaagcg gaactaaata agaaaacact atcgagaacc gaaaaagcaa aaggttatcg
    19441 atataagttt aatgtcgcaa gtttgctaag agctgacact caggcaaggg gacaattcta
    19501 tcaaatcatg cgacgaggcg gagcatactc agcgaatgat gtccgaagat ttgaggactt
    19561 gcaaccgata aataaaaccg gtatggatga ttaccatatt tccggagacc tatatccaat
    19621 cgatatggat ccaacattaa gaaaaacaac ctcgtctaaa agcgtagccg aaaacggtta
    19681 ggttttttta gtttgcaccg aagggaggtg gaaaaatgaa aaaagtgacg ttaagcggcg
    19741 atgtcgtgga taacgatacc gcgtggcttt atgactggtt tgggatcaat tgtatctcac
    19801 cagggaaaat ttctgccgct cttacagaag cagcggggga tgaagtagaa cttgatatct
    19861 catcgaacgg tggggatgtc ctagcggcaa gcgaaatata taccgctatc cgtgcctatc
    19921 cgggaaaggt atccggaaat gttgtgagta ttgcagcaag cgctgcaagt gtaatcgctt
    19981 gcgcttgcga accgcttaga atctcaccta cggcacacat catgattcat aacgcatggg
    20041 tgaccactag tggcaacgct gaggaattaa aagccaatgc agaaatgtta agcagtgtgg
    20101 atgagtctat tgttaatgct tacgagatca aaacaggact cgatcggaaa aaacttgctg
    20161 atttaatggc gaaagatact tggttaaatg ctcaaacagc agtagcagaa gggtttgcag
    20221 atgaaattat gtttgcagaa gcaccagtaa cggtactcaa tgcctctcaa ccggttattc
    20281 caaaaaacgc agtaactaag ttgaaaaatt taatactcaa agcggaagca ccgcaagaag
    20341 aaacactctt acagaaaaaa ctaaaagcct taaatggagg gaaaaacgaa tgaatttaga
    20401 acaattaaaa aatgcgtggg tcgaggcggg aagtaaagtc tctgacttaa atgcacaact
    20461 caatgcagca ttggttgacg atgaaaaaac agaagaagat gtagtaagtt tgcaagcaca
    20521 agtaaaagca gcacgggcta aacgggacgg attgaaagag caagtggcaa atatggaagc
    20581 cgaacaagtc ttaaacgtca aaaaagaacc attagataaa aaagatgaaa acttgaaaaa
    20641 caagtttatc aaagacttta aagcgatggt caatggtgat cctgctatta tggctacttt
    20701 gacatctaat acggatgaat ctggtaatgc tatcggattg actattcctg tagatgtgca
    20761 aacgactatt catactttgg ttcgtcggtt tgactctttg caagaatacg taaacgttga
    20821 aaaagtgacc actaccagcg gttctcgcgt ttatgaaaaa tggtctgata ttacaccgtt
    20881 gactgctttg gatactgaag acggtgaaat cccagcaaat gatgatcctg cactttactt
    20941 gatcaaatac ttgatcaaac gctatgcagg tatttccaca gtaactaaca gcttgctaaa
    21001 agatactgcc gaaaacattt tggcatggtt gtctaaatgg atcgcgaaaa aagtagttgt
    21061 tactcgcaat acaaaaatct tggcagctat tgatggaatc aaagcggcac aaaagaaaga
    21121 tgttacagat gttgatggaa ttaaagatat cgtaaacgtc caacttgatc cagctatcga
    21181 agctacatct atgtttatta caaaccaaga tggcttcaat gttttagata aagtgaaacg
    21241 tgctgatgga tcttacttgt tacaaaaaga cgtagcttct gcaactggat ataccttctt
    21301 aggtaaacca atcaagaaaa ttgcttctcg tttcttgcca aacaaaggta cgcaagctac
    21361 tcctaaatat ccactgtaca ttggtgatct gaaagaagcc gttacattgt atgatcgcga
    21421 aaacatgagc ttgctgacaa cgaatattgg tggtggagct tttgaaacag acaccactaa
    21481 agtacgcgtc atcgatcgct tcgatgtgca actagttgat gatgaagcgg ttgttttggc
    21541 tacttttaca actattgcga acgagacacc ggcgaaagtt taaggagctg attccttatg
    21601 attcttgatc ctaaaatgga tttaggcgaa atcaaaaacg cactaaagat tgataccgat
    21661 gatgacgatg tggaagtaag ccgtgcggca caagctgcaa ttgcatacat tagaggggct
    21721 atcgggaatg ataagccctc tttttataag caagaaaatg aaacggttga tctgattaat
    21781 ttagctatcc tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg
    21841 aacttgcgag agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagttat
    21901 ttgctttttg tggaggagga gtagcgtatg ccccttatcc aaacaggaaa tttaaatcaa
    21961 cgcatcaagt ttgtccgaga tacgacggtt aaggatgagg acgggcaagt tgtcccgact
    22021 tctacaacca ttcttacttg ctgggcaagt gtgcagacac aacgcctgaa cgatattaag
    22081 acgtcgattg gtacggcttt ggaaggaaca ctgacgttca ttatccgcta ccaacaaaaa
    22141 tcagagctga ccaatgatat gaaagtgcgt tggaatggaa aaacgtttga aatcattacg
    22201 attacgaaag gcgagtttgc gaaggacttc acgacaatca ttgcaaaaga ggtttcaaaa
    22261 tgagtgtaga agtcgatgca accgaagtgt acaaagcgct tagggaagta aaagcaaacg
    22321 ttcaacgagt ggaaagccca gcacttagaa aagctgggga gtacgctcaa gaagagttac
    22381 gacaaaacac accttactgg gatggaacga agtcaaacgg taaacgtggt tcgtatatgc
    22441 aagaacatgc taagaaccat gtggttacaa gctcgataaa aaacggattg gtagaagtcg
    22501 gctatgacaa agatgtttct tggcggatgc actttatcga gttcggaaca atcaaacaac
    22561 gtccaaaagg tttcgtacaa aaaacacaaa agcaaatcga aaaacaagta acacaaatca
    22621 ttgctgacga agtaaaaagg aggctaggac tttgaaaacg gcagtatcac aagtctattc
    22681 aattctgaat agcaatgaaa aaacaaagaa cattgatttt tacaccaata gtgttccgga
    22741 atcagctcaa acagtaccta gccttccagt tggcagaatt acagagatat ccggcaacta
    22801 tgaagatttt gcaagcaaca atcctttgac cattcaattt aacgtacagg tagatgtatg
    22861 ggtgtcaacc atgaaagagg ttgatgcctt ttattttgcc cttgatgagg ttatgagggg
    22921 gaatggttgg caatgcgcat acacggaaca aacagatgac gaggacttgg aaggtgcaaa
    22981 gcggattatc aaacgatatg tagcaaatat ttcactaaat taaaaggaga gaaaatagat
    23041 ggcaacagta ggattcgaga gcgtcatttt tggcgtaaaa acaggtgcag gcggcactct
    23101 aaaagaatta gtagcagata agtcgaaagg cggagcgatc gaagctaaaa ttactggatt
    23161 aggcgcaact tctaacacaa catacgcttc aaacgtaccg ttcttcattg caagtaaagg
    23221 ggtttcgtcg ccaaaagtta cgcttgacgt ggcagactta atggataacg gcatttacag
    23281 cgaaatcatt ggtgctaaaa ccgtggatgg tgcaaatgta attggttcag aaactgaagc
    23341 gccttacgtg tcggtagtca tggttacagc gaacaaagaa ggaaaacgct tgttcatggg
    23401 attgacaaaa ggaaaattca gtcatccaga tatcgacatg aaaacagctg aagacaaagg
    23461 ggtagaattg caaaccgatt ccatcgaagg ggaattcatt tctgatgaac gtggctatgt
    23521 atacttaaca gccgtagaat cagaaggaat gaccttacaa aaattcaagg acttggtaaa
    23581 taacaaagag ggggagtagt taaccctgca tctacaccaa cgacagatac agggacacca
    23641 aaagaaccag aaccaaaaat tgatacacaa ggttagccat ttttggctag ccttattttt
    23701 tgtaaaaaca aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa
    23761 aaacattcaa acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg
    23821 agaaagcgga aaaagcagac ctaactgatt tagaagcgat tgattcaatg attgcaatta
    23881 cagcagatat tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg
    23941 cgagcgagtt agtaccggca ttagaaagtg tttttgaaca aataaatgaa ctgggaaaca
    24001 atgaaaaaaa gcagacggcg agcaaaaaga aataagtttt tctgaagcta ggaaagcaat
    24061 ggatcaaatc tacaaagatt taatcgaagc aggttggacg atgagagatg tggacgaagc
    24121 cgactatcat tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga
    24181 tggtgctgat ttcatcaaac aatttttatc ggctgaagac ttagtaaaac ttgaggaagg
    24241 aggtaaataa tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta
    24301 gatagcacta gttttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag
    24361 tcgaatatgc gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt
    24421 agtcaaaaga cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa
    24481 ttaacaaagc gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct
    24541 aaccttaaca cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg
    24601 aacgacacaa aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaatgaa
    24661 atcaaagaaa atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat
    24721 gaatctggtg cttttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag
    24781 aaagctatcg aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca
    24841 gcaaatgaaa ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata
    24901 tctagcaggc aattagaagc actcaaaagc tccagcgatc aatcaggaaa agaaatagaa
    24961 gattttggcg acaagtccac aaggtcagct aggaaactgg acggactaaa agacaaatta
    25021 agctcgctaa aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt
    25081 agcagtgtag taagtggcgt gcaaggtttg gttggcgaag cagtaaacgc atcggattca
    25141 ttgatgaagt tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc
    25201 tcgaaaaaag aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg
    25261 aacacaaccg cacaattggc atctaatggg attcctaact atacagaact aaccaaggcg
    25321 gcaggtaact tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg
    25381 atgctaacgc agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca
    25441 gatgcgatac cgggtgcttc aggattgtta caagacgcta tgttgaaaaa cggagcttat
    25501 acaggaaact tccgtgatgc aatggcgcaa ggtcaaatca cttccgacga gttcaaccaa
    25561 gcaattgtac agttaggtat gaatgacgga gcagttaagg cagccacttc tacagacaca
    25621 ttgagcggtt cttgggagca gatgaaatcc actgtaataa atgggctaca aagtattata
    25681 gaaaaaatag gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa
    25741 aattctattc ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc
    25801 gttgaaaata gagagccact aacatggatt gtcggaatca taggcggaat tacattagca
    25861 gtaaaagcat tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc
    25921 ccttttgtgg cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat
    25981 acaaaatctg aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta
    26041 gttatgagcg ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag
    26101 tggctttggg aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg
    26161 aagaacagcg ttgtgaatac ggttcagaac ctatggaatg gcgtgaaaaa cttcttcagc
    26221 aatggcgttg gagatacgtg gaataaggta gtcggttggg taaaaaacat tttcaacaaa
    26281 gcaactgaat tgaagaacaa cgtttctgat gtaatcggta acctgtggaa tggtatcaaa
    26341 gacacattcc gtagaggtat cgatacggta ttcaattggt tttcagaact accaacgaag
    26401 atgaagaatg ccattattgg cggtaaaaac gccattgttg atgcgttcaa aagtattttc
    26461 aacgcagcac ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg
    26521 gtactagaaa aattgggcgc agaacctcta caagaatggg atgtaccaca atacgctaca
    26581 ggtacaccag caggtgggca cccaatcaat ggtccaatga tggttaatga tggacgtgga
    26641 gcagaaacag ttatcacacc agatggtaga gccttcattc ctaaaggacg caatgttgtt
    26701 ctaaacgcac caaaaggaac gcatgtcttg acagcagaag agaccgcgca acttcaaggt
    26761 tctaaagctc ctaagtatcg ttacaaaaaa ggtactaact tctttggtaa catgtgggat
    26821 agtgtgaaaa aggttgctgg taatgtaggt cacacaatta aaaacgtagt aggtgacgtg
    26881 tgggacttta tttcagaccc gggagcgtta gctagaaaag tacttggggg gttagatgta
    26941 ttaggtggat tgacaaagta tccattagaa gtaggtaaag gcatcctatc taaagcaaca
    27001 agtgcactga ctgaaaaaat cactgggttg ttctcatctg gtaacttaga tacctccgta
    27061 ggaacaaatg gcgtctataa atatttggct gatgttgcta agtctgtgat gaagaaattc
    27121 ccaggatttg tggcaactag tgggtataga ccaggtgacc cttattcaca tggtaaacgt
    27181 aatgccattg atattgcatt accaggcgtc acaggaggct cacctagata cacagaagca
    27241 gcaaactacg cttttgacaa attcgcttcc aaaattggtt acgtaatcac taatgggaaa
    27301 gttcgtgacc gttcaggaca atcaggtcaa ccagcaactg gtgcatggga gccatggccc
    27361 gatggagatc actatgatca tgtgcattta aacggtgtga aagacccaca aaacactcaa
    27421 atttcaggag atagcgtggg aggcagtggg gtagaaagat ggcgcaatgt agcaattaga
    27481 gcgttgaaaa tgaccggtca atacagtact gcaaacttaa atgcattact aaatcaaatg
    27541 cgtacagagt caaatggtaa tcctaatgca gttaacaatt gggatattaa cgccaaaaat
    27601 ggaacaccat caaaagggtt gctccaagtg attgacccaa cattcagaca gtatgcaatg
    27661 ccaggattca acagcaatat ttttgaccca ctatctaaca tcttagcttc aatcagatac
    27721 gcactatcaa gatatggctc actaacaaat gcctatcgtg gagttggtta cgcaaacggt
    27781 ggaattgtaa accaacatca aattgcggaa atcgcagaag gaaacaagcc agaaattatt
    27841 attccgttag ataaggctaa acgatcaaga gcgatgcaat tattgtctat cgcaatggac
    27901 aaactaggag tttctcctaa aacttatgga aatgcgacaa ctgtttccag tgattacgaa
    27961 atcctagaag ccattgaaaa acaggcggca acaacgaatc agctgttatc gttattgctg
    28021 gcatttttca aaggaaatag ccgaactgat agagatttag ctttagatat tcagaagatc
    28081 ttggttagga ggatgtagat gcgaactgta ttactgagaa atagaataaa cgaagaaatt
    28141 gatttgtcca cagaagacta ttttgctact gggttgagta atatggggtt tgaagtaaaa
    28201 aaagaacatg tgggacagtg ggggaatttc agagaaagca gtgagagcgt tgaaatatct
    28261 gaatttcagt catctgtaat catttctgtg catgggtttc gagaaaaaga actgtacaat
    28321 tcacttgtgc agttcctatc ggaaggtccg tttgaactgg aatttgcttt tgacggcgaa
    28381 acaatggtaa gaagatgcag tctaaaatct ttaagtaaga ctgaaatcga tccgaaaaca
    28441 tcgttactaa cagacacttt agaactatat ttcacatcaa actggtattc cgtaaaacgc
    28501 gaaaaattaa ttcagcgacc gaatgtagtg aaaacacgtg gtaaggtttt tccgtataaa
    28561 agatcttata tctacactca aaacttgtgg gagaaaaaag gtgtattcaa attcaataat
    28621 aattctgtat acctaacgaa tagcaaggaa cggatgtccc cgctaaaaat tcgtgtgatt
    28681 gggaagtgtt caaatccgta ttgggaagta atccaaaact cacaaatcat cgcaacggat
    28741 ggatacttca tagatatgac tgaaacacag actctagaag tatcaagcct ttttgaagat
    28801 acaacggcga ttttaaaaga tattgcaggg gtagaatctt ctgtctacca acaacaggat
    28861 tatacaaaaa ctaattttgt tcaggcccca actggagaat tcagcattgt gtttcatgtt
    28921 gggggggcag acgtagagat tgagttatac gaggagcgtg atctgttttg attttagcag
    28981 tcacgctttt tcatcgtgat ttaatgcttt ataacgaaca tttattctct aacagttttg
    29041 aatttggtgt ggacgagatc aacgaagagg ctagtagctt tacgatggat aagtacgttc
    29101 ctgtaaaaac aggcgatttt ctactagcaa aatatattcc tagtggaaaa tttgcatatt
    29161 ttggtgtaat tacgtcgcag gaagacgaaa aaatcagttg taaaagctta cttagtttag
    29221 ctgatagtga gataccgact gcacgtgtgt caggagataa ctacgaagag catatccggc
    29281 ggttgattga atactatttg ctgaatgatc caacgaaaca actaaaagat attttagacg
    29341 tcaaagcaga aagtgcgacc tctcattcgt atcaagctac cgatacgaat aaacacaagt
    29401 taagtgcgta tattctcaat ggtttcaaaa aatacaacgt aaaatggtat ttcaagggaa
    29461 ttcaaaacag aaaaatttat acaggcatac gtgctgtaaa tgaatcaatt tacattaaag
    29521 acaattcttc tgaatttagc gattgggatg tgtttgttca ggcgccgggc gctggaaacg
    29581 agaacaagct attaatcgtt gataaagcaa tgaaagatat agagaaaccg ataatactgt
    29641 caacatggta tttggacgaa gaaaacaatt tgacacaaga tggatcgaaa gaaaatatca
    29701 cgaaaccaac tgtcaatttg gttaatatct acgatcaaac cgcagaagat aaagcatcct
    29761 acgaagatgt ggcaaaatca gagttgaaag gcaatacgta ttcgcatgaa atcaaagtga
    29821 acgttgtaag aaatgcaaaa aatttgaatg tcgaaacgat tgaaacaggg atgtttgcca
    29881 cgatttctta taaaggaaag atatacaagt cggttttgac agcttggcga atatcaagtg
    29941 ataaggagtt tgtggaattg actttcggaa acataagaag tcgttttatg gattattttg
    30001 aagataatgg gggataaaaa atggttagca atgtggatgg atatcaattt gaaaacgtga
    30061 aagtaagcgc agaaaatgat gctagacttt atcacgtttt atataatcgg aaaaatcagg
    30121 ttattgatgg ttacgatcag tctatgaatt tatcttcaag cggattaaca gtaaaagttg
    30181 ctgcaggagc agcgattatt caaggtcgta tggtcgttgt tcgacaagaa gaaagtataa
    30241 cagttccagc aaactcaagt ggttatatag cattaacagt ggatttgaca caagaagtta
    30301 tacctggatc tattcttcca gaatcggaag aatatgaatg gactaataat caagtcaagc
    30361 tagaatttat aacaaaagtt ataaaaggta atttaaacaa tggtgataag gtctataatt
    30421 taccactatg ctcagttaat tctactggat caactgtttc aatctcaaag atatcggata
    30481 gttacgagct gactctctct aaaggagaaa ttttgtggag ggggactgcg ttaatgcatg
    30541 atactcaaac tgtccaacct tcaaaaaaaa tttggcagac agtttcaggt tttttgttag
    30601 tatggctccc atacgaaaac gggcaagcaa ttgaggatag atatgtaact acgccttttt
    30661 ataaggagcg tgtaactttt actaatgttt taggagaaat tgtttcagga tttgatgact
    30721 atcacaaaaa gtggtttagt aaacgaataa actataattc gaatactaat atatttacag
    30781 gtgcagcaag caatgcaagt ggagataacg caaatatggt gcttaggtat atagtttctt
    30841 tttagttagg aggtatggaa gagtggcaaa tttagaaatt aaattatctg caaataagag
    30901 acagccttat ctacgtcacc gtgttgttgg tagagttggt gatggggggc tcacaacaat
    30961 caatgtacaa cttcttgagg aagatgaaat tacacctttt gtaattaatc taaacggtac
    31021 tttgaaattt gtgggcgaag tttcaaacgg taactatacc gagggagaac cagaaataat
    31081 cgattcgact aatgggttaa ttagttacac gttcactaag tcaaatttca gcacgagtca
    31141 tcaattcaaa caagcatatt ttgaatatgt agatcctaac ggcaaaaaag taacttttca
    31201 gaacttcatc atagacgtgt tagaacgagt ggatattaat tcggagcaag cgaaatacta
    31261 tatttcttca ttggaaaaat tacagagtga aatgcaaacc actttcaatc agttcattag
    31321 tgataaacag gtccaatacg atcaaatcta ctcgaaatat aacgaattag taagattgat
    31381 aaatgaatca gataagcaag taaatgatcg tattgatcaa accaatcagc aaatcggcga
    31441 tctcggcaag ctgaaaaaga tgtacagtaa cagcatcgac tttgggggct atgattattc
    31501 ggggaatccg aatttgttaa gtaagctatc atacgactta attgaaaatc aaaatacttc
    31561 agctggaaca ctttctaagg gtgaaaactc gtttaaatat aataagatat cagctgaagt
    31621 ggaaggtgga gtagagttat attataaacg aagaggtata gctaactggt taccctctaa
    31681 taaaacgctt gtaatgactg ttaagcttag agctggagcg gactatagtc cagttgacgg
    31741 aaaacttata ctgattagat ataggtatgt tgacagtgga actggcaaga ttgttttaga
    31801 cttacctatt aacagtaatt cgataactca ggaatggaaa gagtttagta ttactggaac
    31861 tactccaaca tttagcccac aagcatacca tccttggata caatttaggg ctcaagatgg
    31921 ggtacttggg gaaatagaaa tgagctatga catcaaaatc gaagaaggct ctacagctac
    31981 accatatcag cctaacttac ttgatgactc ttactggcta ggtaaaacac cgttgggtga
    32041 gaatttgata acgaatgcca agttcccaat tataacacga agcaatccta tttctggttt
    32101 tgatatttca gaagaattaa taataggtga aacttatacg gtatctttaa aagggactaa
    32161 gccagctact aaagaattcc acttatacta cggagaagca aattaccagc agttgcagta
    32221 tcaggcaact ttacttcctg tggaaggatt aacagatgtg tggagtgcca cttttacagc
    32281 cagaaatact agtgaaacga caaatttact tagagtagcg ttatggcaaa aacctaattc
    32341 tgcaacatat gatactgttc aaattgactg gctcaagatt gaaaaaggcg acacacgcac
    32401 accaaatatc agtcagttta aatactttgg tgaaggcttg aaagacagta acaatccgaa
    32461 cgactacagc tgggatgtca caccagagta tactgaaaaa agcttgaata atacggttag
    32521 tctgactgaa ccgcaatcca tcgaaggatt gaaaaatttc gaagatggta ttcaatcaaa
    32581 aggaaaatct gtattgacat cagacgacaa caaatatgaa gtcgtaacct taacagttac
    32641 aaacgggaat accggatcag caaagcttta tcgtgaagga aaaaccgtca ctatttattt
    32701 ttttgcgttg aatgggaaaa gcagtggtgg aaatgattca acgatactaa caattccaga
    32761 aggctatcgg ccaccaatta gttttgagca actggttggc tcgatagacc gttctacttt
    32821 gaacagtgct cagttatcta ttggtgcaga tggagccatt aaatggcgaa gaaactcaag
    32881 ttatggatcg gattatacct ttgcaattac ttacacgatt tagaaaggcg tgaatcgata
    32941 tgaaggcagc atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg
    33001 aagaacattt accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga
    33061 aattcaatta ctgggaggga aataaatgaa aaacatttgg aaatatggcc gtactggcgg
    33121 agagtatgca ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc
    33181 accgctcgaa ggggttcgtt cagatggcga accgctaacg attgctgatc agatgtttga
    33241 tcctaaattg aaccaatgga ttattttagc gaacgcgtta gatcacaacg atttaaacaa
    33301 tctcaaagcg atgtacgagg ctcttgaaca tgaaaacggc aacctaaaac agctaaatgc
    33361 caaactcatg ctaagcgatg tagcgattaa acaggaaaat actgcattga aagaaaaagc
    33421 tgacagttta gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga
    33481 catttcagaa attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta
    33541 gttttagcga tgtgaaaatg atgtatgatt ggggctgttt tactgacgat caagttcgtc
    33601 tattcgttcc actatgtatc acagatgaag aagcagatag aattattagc aaagaagaga
    33661 gcgcatctta agtgatgcgt ttttttgttg gaaagttggt ggaacatgaa agaagaagcg
    33721 ctccaagacg ttgtggagag attagtaaga attgaaacaa aattagacaa ctacgaatca
    33781 cttagagaaa aagctgatag tgcaaaagat ttggcagaca aagcctactc agtagcgcta
    33841 aacaacgcag aagatatcaa agaaatgaaa gccaataata aatggtcgtg gggttacatg
    33901 atcggtttag gcattacgat cattggctat ttcttgacta aattgtaaag gagttaagaa
    33961 gaaatgattt tacccgataa gtattatcaa gtcattaaat ggacggtttt aacagtatta
    34021 ccagctgcat ctgttttagt agccacgtta ggaaaagcgt atggatggaa tggaacagat
    34081 atgacagtac tcactatcaa tgcagtagca acatttttag gcgttatcac tggtgtgtct
    34141 gcatataact taaaaaaata ggaggaaaaa aatgaagaag aaaatcattt tatcattgag
    34201 cctaataatg gctctttttt tattgccaat taacgggttc gcctatacga tcaacaatga
    34261 atttaattta ggtgtaaatg aaggtagctc acaagtagca aataaccaat acatcctatt
    34321 gcatgaaaca gcaaacgaaa ctgcgacagg acgaaatgaa gcgcagtaca tgaaacgttc
    34381 ttggtacaat gcctatacag cgtatattgt tggcgatggt gggatcgttt accaagttgg
    34441 acaacctggt tatgtacaat atggcgctgg ttcatatgct aatgcaaata gtcctgtgca
    34501 aatcgaactg caacatacgc acgataaagt aacgtttgaa aagaactaca aggcatatgt
    34561 tgaattagcg agagattccg ctaataaata cggtattcca cttacattag acacaccgta
    34621 taatcaacca ggaatcaaat cacatttatg ggtaactcaa aacatttggg gcgatcacac
    34681 tgatccgtat ggatacttat cagaaatggg tgtaagtaag gaaaaactgg cttacgattt
    34741 agctcatgga tttacagacg aaaacccaac gacttctgag aacaagcctg tcattgatcc
    34801 aacccgagca ggtgcagcaa atccaacttt atcagatgga acgaaccatt ctcacattga
    34861 tcagtttgga gaaatcgaaa atgcgaactt acacgtcgct ggatggcaca tcgctaacta
    34921 taaatacgag tatattttca ttatggacta caatactggg aaagaattag ctcgagtaag
    34981 agctgatgga atttatagac cggatgtaaa tcaagcttat aatacttctg gaaacgttgg
    35041 ctatcatgta tcttttaaca tgcgcaattt ccctaataag aaagtatacg tcatgatgcg
    35101 ggcaacgaat gatccagaag gaaacactaa aggcggtgcg caagatttcc atgacaaacg
    35161 ttggtattta aatattccta aacgataaaa atagctcctc gttgaggagc agtacataga
    35221 attgaaaatt aacgttaaat caaaaaaata tttacaagat gtttatttaa gtagataata
    35281 aaagaaaaaa gcaggtgaaa ttgtgacagc agaaattggt attatgaata aaagtggaat
    35341 cgtgttagct tcagattcag catctacgat tggagatagc aaagtatata atactgcaaa
    35401 aaaattattt actttggatt ccatgcattc tgtaggtatc atgatatatg gtaatgctga
    35461 atttaatggt attccttggg aaataatcat tactcaatat aagaaaagca ttggtagttc
    35521 tgtttttaat actttggaag aatacgcgga taattttata gaatttgtca aaacagcatc
    35581 ttttattaga agtgaacaga ccgagcaaga acaaatgatt ggggtttttc aaaaaataat
    35641 ttcaggttta tttgagagta tagaagtaga tattaacttt ttaataagcc aaggtacaca
    35701 aatagacaaa gatgtcctag tgaagttatt acaaacaaaa atgaatacca atttgtctca
    35761 acagtcccag acattcattt tagatattga aaaggcgtta tttcttagta actatggaga
    35821 aatattaaaa gatattctta atagtatttc tacaatggaa ggtgtttcag aagctatatc
    35881 agaggaaatt caaagttacg tttatgaaat tataattaga gacgatgttt attcttcccc
    35941 tacaggaata gttatagcag gatatggaag aatggatatt ttccctaaac tatattctta
    36001 taatatgttt ggatttgtta tgaatattct gaaatactca gaatatgaaa gtgctcaaat
    36061 aggaaatgac aatggttcat taagatctac tatattacca tttgctcagt ctgatgtggt
    36121 aaatactgta gttcaagggg tagatccaca aattactaat tatctgtcga gtcaagtcga
    36181 tagttttgac gataacggaa aaaataccta tattaatatt ataaagaata tttcagagtt
    36241 tcaacaaaat caattcatat ttccgttact aaacatgatt gctttactcc cagttgaaga
    36301 aacagcaatt atcgcagaga ctttattaaa tttaaccagt tttaaacgaa aatatactac
    36361 ttctgtagaa acagtaggtg gcccaattga tgttttagct attacaccta atgatggccc
    36421 tatttggatt aaaagaaaac attattttga tattgataat aatattggat acagattgcg
    36481 aaaggagaga gcgaatgatt acaataatta aacctcagga aaaatcccct gttttgaaat
    36541 ttaatgagag caatctagcc cataataaaa tgagtataac tgatacagta tttgatgtaa
    36601 tggttgagca acaaaaaaaa attaataata acaatagaca aagaaaagaa attaaccaat
    36661 attaatgttt tttctgttgc gttttttgca gtcataattt aaaacctcgc tcaatcttgg
    36721 gcgaggtctt tttttatttt tctgaaaagt taaagtaaat aatagcataa aataatatat
    36781 tttacaaaga ataagtacaa tctagttttt tgctattaaa tgtgtaataa taaatatgcc
    36841 atcacaacat gaagaatgaa atccattatt atctagtcta gtccattctt tttgtttgca
    36901 gtatttgtga tggctttccg tacccttagc tcagttggtc agag
//
LOCUS       DAZKCC010000019        34687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000019 DAZKCC010000000
VERSION     DAZKCC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.019"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG8VH_001046"
     CDS             591..1280
                     /locus_tag="ACG8VH_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9109620.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG8VH_001047"
     CDS             1267..2475
                     /locus_tag="ACG8VH_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9109621.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG8VH_001048"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG8VH_001048"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9109622.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8VH_001049"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8VH_001049"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9109623.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG8VH_001050"
     CDS             complement(6578..7273)
                     /locus_tag="ACG8VH_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP9109624.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8VH_001051"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8VH_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9109625.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG8VH_001052"
     CDS             complement(8472..8663)
                     /locus_tag="ACG8VH_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP9109626.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG8VH_001053"
     CDS             complement(8684..9574)
                     /locus_tag="ACG8VH_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9109627.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG8VH_001054"
     CDS             complement(9561..10328)
                     /locus_tag="ACG8VH_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9109628.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8VH_001055"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8VH_001055"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9109629.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8VH_001056"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8VH_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9109630.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8VH_001057"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8VH_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9109631.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG8VH_001058"
     CDS             14899..15282
                     /locus_tag="ACG8VH_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109632.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8VH_001059"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8VH_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP9109633.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG8VH_001060"
     CDS             complement(16226..17044)
                     /locus_tag="ACG8VH_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP9109634.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8VH_001061"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8VH_001061"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9109635.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8VH_001062"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8VH_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9109636.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8VH_001063"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG8VH_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9109637.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG8VH_001064"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG8VH_001064"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9109638.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG8VH_001065"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACG8VH_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP9109639.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACG8VH_001066"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACG8VH_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9109640.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG8VH_001067"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACG8VH_001067"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9109641.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG8VH_001068"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACG8VH_001068"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP9109642.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG8VH_001069"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACG8VH_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9109643.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACG8VH_001070"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACG8VH_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9109644.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG8VH_001071"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACG8VH_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9109645.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACG8VH_001072"
     CDS             28352..30325
                     /locus_tag="ACG8VH_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFP9109646.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACG8VH_001073"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACG8VH_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9109647.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG8VH_001074"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACG8VH_001074"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9109648.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACG8VH_001075"
     CDS             33204..34496
                     /locus_tag="ACG8VH_001075"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9109649.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10569 a         6743 c         6858 g        10517 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaa
//
LOCUS       DAZKCC010000020        34611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000020 DAZKCC010000000
VERSION     DAZKCC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.020"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..903)
                     /locus_tag="ACG8VH_001076"
     CDS             complement(55..903)
                     /locus_tag="ACG8VH_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9109650.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1127..3067)
                     /gene="thrS"
                     /locus_tag="ACG8VH_001077"
     CDS             complement(1127..3067)
                     /gene="thrS"
                     /locus_tag="ACG8VH_001077"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9109651.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3404..3583)
                     /locus_tag="ACG8VH_001078"
     CDS             complement(3404..3583)
                     /locus_tag="ACG8VH_001078"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP9109652.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3630..5453)
                     /locus_tag="ACG8VH_001079"
     CDS             complement(3630..5453)
                     /locus_tag="ACG8VH_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9109653.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5731..8010
                     /gene="helD"
                     /locus_tag="ACG8VH_001080"
     CDS             5731..8010
                     /gene="helD"
                     /locus_tag="ACG8VH_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP9109654.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8103..8750)
                     /locus_tag="ACG8VH_001081"
     CDS             complement(8103..8750)
                     /locus_tag="ACG8VH_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109655.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8929..9648)
                     /locus_tag="ACG8VH_001082"
     CDS             complement(8929..9648)
                     /locus_tag="ACG8VH_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9109656.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9779..10930)
                     /locus_tag="ACG8VH_001083"
     CDS             complement(9779..10930)
                     /locus_tag="ACG8VH_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP9109657.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11147..11887)
                     /locus_tag="ACG8VH_001084"
     CDS             complement(11147..11887)
                     /locus_tag="ACG8VH_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9109658.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11891..12214)
                     /gene="rsfS"
                     /locus_tag="ACG8VH_001085"
     CDS             complement(11891..12214)
                     /gene="rsfS"
                     /locus_tag="ACG8VH_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9109659.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12271..12864)
                     /gene="yqeK"
                     /locus_tag="ACG8VH_001086"
     CDS             complement(12271..12864)
                     /gene="yqeK"
                     /locus_tag="ACG8VH_001086"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP9109660.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12848..13498)
                     /locus_tag="ACG8VH_001087"
     CDS             complement(12848..13498)
                     /locus_tag="ACG8VH_001087"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9109661.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13508..13819)
                     /gene="yhbY"
                     /locus_tag="ACG8VH_001088"
     CDS             complement(13508..13819)
                     /gene="yhbY"
                     /locus_tag="ACG8VH_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9109662.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13835..14944)
                     /gene="yqeH"
                     /locus_tag="ACG8VH_001089"
     CDS             complement(13835..14944)
                     /gene="yqeH"
                     /locus_tag="ACG8VH_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP9109663.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14941..15468)
                     /locus_tag="ACG8VH_001090"
     CDS             complement(14941..15468)
                     /locus_tag="ACG8VH_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP9109664.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15648..16433)
                     /locus_tag="ACG8VH_001091"
     CDS             complement(15648..16433)
                     /locus_tag="ACG8VH_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9109665.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16426..17295)
                     /gene="accD"
                     /locus_tag="ACG8VH_001092"
     CDS             complement(16426..17295)
                     /gene="accD"
                     /locus_tag="ACG8VH_001092"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9109666.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17296..18675)
                     /locus_tag="ACG8VH_001093"
     CDS             complement(17296..18675)
                     /locus_tag="ACG8VH_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9109667.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18683..19102)
                     /gene="fabZ"
                     /locus_tag="ACG8VH_001094"
     CDS             complement(18683..19102)
                     /gene="fabZ"
                     /locus_tag="ACG8VH_001094"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9109668.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19127..19603)
                     /gene="accB"
                     /locus_tag="ACG8VH_001095"
     CDS             complement(19127..19603)
                     /gene="accB"
                     /locus_tag="ACG8VH_001095"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9109669.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19606..20841)
                     /gene="fabF"
                     /locus_tag="ACG8VH_001096"
     CDS             complement(19606..20841)
                     /gene="fabF"
                     /locus_tag="ACG8VH_001096"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9109670.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20860..21597)
                     /gene="fabG"
                     /locus_tag="ACG8VH_001097"
     CDS             complement(20860..21597)
                     /gene="fabG"
                     /locus_tag="ACG8VH_001097"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP9109671.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21609..22526)
                     /gene="fabD"
                     /locus_tag="ACG8VH_001098"
     CDS             complement(21609..22526)
                     /gene="fabD"
                     /locus_tag="ACG8VH_001098"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9109672.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22633..22857)
                     /locus_tag="ACG8VH_001099"
     CDS             complement(22633..22857)
                     /locus_tag="ACG8VH_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9109673.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22910..23872)
                     /locus_tag="ACG8VH_001100"
     CDS             complement(22910..23872)
                     /locus_tag="ACG8VH_001100"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9109674.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23882..24322)
                     /locus_tag="ACG8VH_001101"
     CDS             complement(23882..24322)
                     /locus_tag="ACG8VH_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9109675.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24628..25593
                     /locus_tag="ACG8VH_001102"
     CDS             24628..25593
                     /locus_tag="ACG8VH_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9109676.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25650..25880)
                     /locus_tag="ACG8VH_001103"
     CDS             complement(25650..25880)
                     /locus_tag="ACG8VH_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP9109677.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25966..26853)
                     /locus_tag="ACG8VH_001104"
     CDS             complement(25966..26853)
                     /locus_tag="ACG8VH_001104"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9109678.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26942..28165)
                     /locus_tag="ACG8VH_001105"
     CDS             complement(26942..28165)
                     /locus_tag="ACG8VH_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9109679.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28182..29228)
                     /locus_tag="ACG8VH_001106"
     CDS             complement(28182..29228)
                     /locus_tag="ACG8VH_001106"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9109680.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29392..31119)
                     /gene="ptsP"
                     /locus_tag="ACG8VH_001107"
     CDS             complement(29392..31119)
                     /gene="ptsP"
                     /locus_tag="ACG8VH_001107"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9109681.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31119..31385)
                     /locus_tag="ACG8VH_001108"
     CDS             complement(31119..31385)
                     /locus_tag="ACG8VH_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP9109682.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31864..34098
                     /locus_tag="ACG8VH_001109"
     CDS             31864..34098
                     /locus_tag="ACG8VH_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9109683.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34246..34566
                     /locus_tag="ACG8VH_001110"
     CDS             34246..34566
                     /locus_tag="ACG8VH_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9109684.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9777 a         7486 c         6042 g        11306 t
ORIGIN      
        1 tggaaaacta cgttttgtta ttctaaaaaa cttatataaa aaatagtaag gtttttattt
       61 cgaatccata aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt
      121 ttcgttttta gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag
      181 tgaattagtt gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac
      241 atcggctatc ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt
      301 catgcttttc gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac
      361 aagccaaagc actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc
      421 tttttcaaaa atgattggct taccgacacg agaccatttt tgcagaatat cttctactac
      481 taaagaagag gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac
      541 gactttttgg atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc
      601 attgttacca agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa
      661 accttcatcg attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact
      721 ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc
      781 tgctaattca ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc
      841 caactttttt tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct
      901 cataagttcc tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat
      961 tcacggtcgt atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc
     1021 catcaatccc acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg
     1081 ttcggattgc tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc
     1141 tgtaattttt gacttctgct tccatagcat caataaagat attcaaatct tctacagctg
     1201 tttctttact tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa
     1261 caactaattg gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt
     1321 cattacgatc atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat
     1381 aagcataatc gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca
     1441 accatgttgg aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg
     1501 tagaaacaat tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa
     1561 catatgtcaa atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt
     1621 cttctgtacc aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct
     1681 cgccttctgc ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt
     1741 gggctttttc ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat
     1801 agctcaagcg gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt
     1861 caagcgtacg tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat
     1921 tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat
     1981 gcatcattcc taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt
     2041 tgtagaccat catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc
     2101 ccatatccat tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt
     2161 acaactcaac atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt
     2221 tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg
     2281 gcaaaccaga acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac
     2341 gatggtctct ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt
     2401 caaaaaaggc tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat
     2461 aagcacctgc cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg
     2521 gtccacgaca caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg
     2581 gcaattcact aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct
     2641 gttttgaaag aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg
     2701 cttcgattgc cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt
     2761 aaaaccctga atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca
     2821 tggcttgggc catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt
     2881 ctggtgtgat gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt
     2941 ttccgttcac tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa
     3001 ttgccaatgt tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt
     3061 ttatcataat agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac
     3121 aaaaaaatcc tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct
     3181 aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa
     3241 caagggtttc gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt
     3301 tcctctctta tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt
     3361 atttaaccac taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc
     3421 catttacacc gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg
     3481 gtgcctgcgt gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt
     3541 ccggactgcg accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt
     3601 ctgatcgttc ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca
     3661 ggaaaaatgg ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg
     3721 acttcttgca aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc
     3781 aaaaaaatac aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct
     3841 cttgaaatca ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct
     3901 gcactttcac tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca
     3961 gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta
     4021 gggatacttt ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct
     4081 aattggactt tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc
     4141 aaatttttca catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc
     4201 tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta
     4261 ttggacgttt ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga
     4321 ttattgacta ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac
     4381 agttggctgt ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt
     4441 tctgcactag ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc
     4501 gcctctactt ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt
     4561 gtacgtgcga gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa
     4621 cggctcattc ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc
     4681 actaccagta cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg
     4741 atcaaataag caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg
     4801 gcaggctttt cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg
     4861 gcacgttttt cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc
     4921 tgatctccag gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc
     4981 gaagattctc ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt
     5041 aactggtcat caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat
     5101 cgatgggcaa ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta
     5161 ttcgcgtaat cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc
     5221 cagtattctt tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca
     5281 tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct
     5341 ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat
     5401 aaagctatcc caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt
     5461 tctcctcttt tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct
     5521 taagcatagt ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc
     5581 actatcagcg tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact
     5641 ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc
     5701 gttatttacg gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac
     5761 gtggatgaaa cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa
     5821 gaattgactc aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct
     5881 ggttccaatg aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg
     5941 cttttgaaat accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg
     6001 aaagatagtc cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta
     6061 tatctaggta tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc
     6121 gcaccaattg ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa
     6181 acagatagaa tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt
     6241 attttatcta tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg
     6301 gatgaatcat caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat
     6361 gcaatcattc gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga
     6421 aagacatccg ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc
     6481 gaggctgaaa acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact
     6541 gtattgcctt cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg
     6601 cagctgctgc cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta
     6661 ggggaaaaag atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat
     6721 cgctatatcc aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc
     6781 cgtacgatcc tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta
     6841 cctttacatc atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg
     6901 caaaaagacg aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa
     6961 ctatacgcaa cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa
     7021 aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat
     7081 tatcaatttg tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct
     7141 cttctaacac gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt
     7201 acacacttgc gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg
     7261 cgccgaacat atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg
     7321 caggatttcg caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg
     7381 actttctgtg gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg
     7441 gaagaccttt tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc
     7501 acaaaagaaa tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca
     7561 acagcaagaa aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg
     7621 aattggttag cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca
     7681 atcatagcta aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg
     7741 aaacaacaag tccagctgat tttaaatgag agtgacttta tgaagcgttc cattatttta
     7801 cttccagcct ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat
     7861 gcagaacatt tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg
     7921 atgcatgaat tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat
     7981 atttcatctt atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta
     8041 gcagttgtct ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc
     8101 atttattcta tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata
     8161 atcattttca gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg
     8221 ccaatcccgc cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt
     8281 gattggaaat tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct
     8341 aggtatgcac tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata
     8401 agaatttttt caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc
     8461 cgatactctt cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc
     8521 agataatttt gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg
     8581 ttcagatacc ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg
     8641 tatttttgcg ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg
     8701 taaccgctta agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc
     8761 gtccatcttc cacatacttc atatcatttc acatgatact tatgctctca tcataccaca
     8821 ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt
     8881 gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa
     8941 tgccacatta tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt
     9001 ttcaaatgtt tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat
     9061 ctcagcgatt gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa
     9121 atccgctact tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc
     9181 gatgtctttt tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa
     9241 gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt
     9301 tttcccaaat gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc
     9361 aacaatcacc attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc
     9421 atctcctgca ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata
     9481 agttttagcg cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc
     9541 tgattttgct gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc
     9601 atctttagca gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg
     9661 ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca
     9721 cgattctgtg aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct
     9781 atactcggat tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga
     9841 tttgatcact tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat
     9901 aaatagaaat ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta
     9961 gtcgaagata atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga
    10021 cataacaaga taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca
    10081 caaaatcctc aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt
    10141 cggtcatctg atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca
    10201 aatacggcca ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct
    10261 gaggcggcac aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg
    10321 cactggctat cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga
    10381 tcggatgtaa ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca
    10441 agatatgatt tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct
    10501 cagtcatttt ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta
    10561 tcctatcttg attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt
    10621 cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt
    10681 tagcaaaata gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca
    10741 caccattttg taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc
    10801 tttgcaaaaa attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg
    10861 ccatttccac atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc
    10921 agcttttcat gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa
    10981 aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac
    11041 ttctgcgacg aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta
    11101 tgttgaagat ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg
    11161 ctacaaagaa ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg
    11221 cttcgacttt cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg
    11281 tacgctcttg atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa
    11341 cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca
    11401 aaaaagcaaa attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt
    11461 gataggtgga gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt
    11521 catcaaacac ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac
    11581 aggtaattgc ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt
    11641 ggatcgatgc gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa
    11701 ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac
    11761 cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta
    11821 accattgctg ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat
    11881 aagccatatg ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt
    11941 tttccagatt atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa
    12001 gatcgatcaa tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt
    12061 tttcttcttc tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg
    12121 ctgaacagat catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa
    12181 tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat
    12241 ctatgatgat ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt
    12301 ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt
    12361 tgtttcaaat gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac
    12421 cccaggaaaa tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag
    12481 actcatctta gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt
    12541 gattcctaat tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt
    12601 ataattcaat aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc
    12661 tggtcgttct tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc
    12721 accgtatttt ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg
    12781 ttgttcgctc attctcatct gtattttttg catcaggttt tctctagaat aagcagtata
    12841 ttcattacta tagtccatat tcatacaacc cttcattttg aatataatca attactttat
    12901 ctggaacaag gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac
    12961 tgatgtcgat ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc
    13021 ctggtcgacg tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt
    13081 ttggcaaata ttctaccata tccccaccga taataaagta ataatctgta tccggattgt
    13141 tttgcgtcaa ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa
    13201 tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat
    13261 gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa
    13321 atacgcggtc aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt
    13381 gaacgggatt gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag
    13441 gcatttcttc aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg
    13501 tcactcctta tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag
    13561 gtttgtataa tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt
    13621 ctaatacttg agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga
    13681 tcagttctct tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac
    13741 cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt
    13801 gttttcctct taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata
    13861 catctacacc ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc
    13921 ctagaccggc aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct
    13981 ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt
    14041 gataaaattc gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat
    14101 aagcaataaa tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga
    14161 aaagtgtttg cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa
    14221 tgatccgcaa atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg
    14281 gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc
    14341 ctggaaactg cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat
    14401 tgataagcgt cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc
    14461 ggtattcttc gattttttct aatagttccg tcatctcatg agatttctta gcactagtta
    14521 ataatacatc tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta
    14581 atttagtttt tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg
    14641 gattgtcacc gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat
    14701 ccaccacatt gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt
    14761 cgtcatccgt caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct
    14821 gacaatagac ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta
    14881 attcaccggg tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg
    14941 tcattcgatt ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat
    15001 tcttttttcc caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt
    15061 tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc
    15121 gcctaccatt actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc
    15181 tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg
    15241 cgcgacacgt tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat
    15301 ttctaaaatc caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt
    15361 attgtctaaa tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat
    15421 ctgatagatg gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca
    15481 tctgcaaaaa atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca
    15541 gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg
    15601 accaaaaata aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg
    15661 aacctcttgt atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat
    15721 ctttcaatca acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg
    15781 ttgaacgttt caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta
    15841 attttcatca actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca
    15901 aatccttctg gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct
    15961 aaacctagag caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt
    16021 acctttaaat cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct
    16081 tctgctccta caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg
    16141 aacttttccg cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa
    16201 ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt
    16261 accggtttgc catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa
    16321 cgatcaccat ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc
    16381 catcgttctt tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat
    16441 ccatcctttc tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg
    16501 gggaacaatc tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc
    16561 tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct
    16621 ttggggttct gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt
    16681 cgtcgggtcc gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc
    16741 agcagagatt tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc
    16801 tgaagctgtg aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt
    16861 gatcttttct ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc
    16921 aataatgaag ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc
    16981 tgttttatct tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt
    17041 ggttacaaga tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg
    17101 ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat
    17161 gtctttcgta taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac
    17221 agatggcgag ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt
    17281 cttaaacaga gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa
    17341 ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa
    17401 atccatctga aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg
    17461 ctgcatcttc attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat
    17521 tgaatcataa tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc
    17581 catcccacct gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa
    17641 agcaggattt tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc
    17701 ttgacacaca ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat
    17761 tcctgtgacc atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa
    17821 ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac
    17881 tgctttagct gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac
    17941 tacagaagga gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc
    18001 gcctaaatga atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc
    18061 aggataaatg attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg
    18121 ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt
    18181 cccgccacca cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc
    18241 caatgcttct tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc
    18301 ttcttgcatc aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt
    18361 aggaccaata aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga
    18421 taaaaaacca aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac
    18481 ttcctgaaca ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc
    18541 tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat
    18601 ccctagttcg cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa
    18661 tactttagaa aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt
    18721 ttttaccgtc tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata
    18781 tctcaacttc aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt
    18841 ccaatccgcc gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg
    18901 ctcctgcttg agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa
    18961 aatgtccttg aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc
    19021 cttcaaccat ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt
    19081 cttttatttc ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa
    19141 tcctaaagag tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga
    19201 tcactccgtc ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga
    19261 taacttctcc tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg
    19321 gttgcagata aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg
    19381 attctttttc tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg
    19441 gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat
    19501 ttttattcat atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt
    19561 gatcaaattg actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg
    19621 tttcatgcac agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt
    19681 aaaacttgca ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt
    19741 ttctacattg atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc
    19801 ttcgatcccg ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta
    19861 agtatctctg aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt
    19921 ggctggtgta cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc
    19981 ttcttccatc gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt
    20041 catatggtaa gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc
    20101 gcgagcttgt gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac
    20161 aaaaccattc cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga
    20221 gagtgctgtt aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc
    20281 tccagcaaga atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga
    20341 attcgttccg ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg
    20401 caaagcaata tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac
    20461 tcgttttgca cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc
    20521 gatcccagat ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc
    20581 gctcatctcc attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg
    20641 tttgctatct tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa
    20701 ggtaatccct gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc
    20761 ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc
    20821 agtaattacc actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc
    20881 gccatcgaca ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt
    20941 ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc
    21001 cttgactttg tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc
    21061 attacatgta atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac
    21121 gccagcttta ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact
    21181 agagagatta atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt
    21241 catattgaag gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat
    21301 tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc
    21361 aagcttttct tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc
    21421 ggaaactcct acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat
    21481 ctctccgcga ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc
    21541 tttgccgatt cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac
    21601 gttcctcctt atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga
    21661 actggtattg ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact
    21721 tcgatgactt gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca
    21781 ggttgcatga cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt
    21841 gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc
    21901 gccagttttt ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga
    21961 gggatcattc ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt
    22021 tcttcgccac caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg
    22081 tacttgctag cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca
    22141 accattttcc cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact
    22201 aacgcaactg cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct
    22261 aaactcagtc ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac
    22321 gcgatgctga ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt
    22381 tctgtaaagc aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc
    22441 tgctttacaa cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg
    22501 ccactaaata agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga
    22561 ttcttcctat tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc
    22621 taattttaat gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt
    22681 gattccgtct tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg
    22741 gaataaatcc aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc
    22801 ttcagtttct tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat
    22861 aatatattcc tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca
    22921 tgagaatact tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt
    22981 ttcctaaggt gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag
    23041 cagatgtatt cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt
    23101 ttcgggcaat tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg
    23161 gtttgtcttt catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa
    23221 tatctctgcc agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat
    23281 ttttattttg ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt
    23341 tttctttcag caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc
    23401 caaacaacac agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac
    23461 caatcaccag tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat
    23521 aaacaaatcc tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt
    23581 tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta
    23641 aaataaaatc caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta
    23701 aaatgcataa atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc
    23761 ttgacgaaat ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa
    23821 tcttttcagg aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc
    23881 ctcacttata gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct
    23941 gttcctccgt agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat
    24001 ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat
    24061 cttcactgcg gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca
    24121 aggtcccaac agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca
    24181 tcccgattgc ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt
    24241 tcagctcaga ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta
    24301 ctgtttttaa atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt
    24361 ttgatgttca aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt
    24421 actatatgag ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat
    24481 aatttctttt attagaaaaa tcatctatct cacatatttt gattatcaaa gtattttctg
    24541 tgaacagaac tatagaattc cctaaaactt gtccattatt tcaaaatttg ttatagtctt
    24601 tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac tctccccatg
    24661 ctgccgggtg tctatcttat gaaagaccga gatggtaagg tcatctatgt tggtaaagca
    24721 aaacggctca aaaatagagt aagcagctat tttcatcaga ataaacagca ttcaaaaaaa
    24781 gtattacgta tgattcatca tataacagat tttgatttcg tcgtagtaga cacggaatta
    24841 gatgctttat tgctcgagtg tcaattgatc caacattacc gtccttttta taaccgacaa
    24901 atgaattatt tcagtaatta taattatgta catattacca ataagggatt tgtattaaca
    24961 gatactccta cggctcgaac atacgggcct tttcgtctct ataaaaaaat gccgtctatt
    25021 ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt ctgaaattag tttgttagct
    25081 ttgcgagtcc agcttcctga cctacaggaa atgacttttg aacagaaaaa aaaggaactc
    25141 caagggctgt ttcaaggccg aaataaaaag ctactgactt acttgaaaaa aagacaacaa
    25201 catttcatct atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta
    25261 gttacttatt ttattagacg gatccaggaa caaaagcagt ttttaagaac accatcgctc
    25321 accttcagca tgccattagc agcagatgag agtcagaaaa agcactacct cgtttgttat
    25381 ggtcaacttg cagaaacaat gattacctct ggtgacgttt ctcctgactt ctattatgaa
    25441 aagaaagaag ctcatttatc tttgaaacgt caattgtcaa aagaagaaat cgatcctgta
    25501 caaatcttga tcagttaccg caaaaaatta gaaaaagaac agtttgaaat tgaactgtta
    25561 aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc tcggcttctt ttaggcatct
    25621 tcaacttcaa atgtcaatta tcctaaaatt tattggatta agtcatagat ttccatcgct
    25681 acgatatcga tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt taattcaaat
    25741 gtatcggtcg ctttatcata agttactgta cacttctcta cgccttcttt ttcaaaacgg
    25801 cgaacttgca gttcattatc tgctgactcg accatagcct ccagtcgttt tatgattgcg
    25861 actaattgtg aatgtgtcat cggaggtcct ccttttctta tttctgttat attgtatcaa
    25921 aagcatagcc tacttgcatt gattttctct aaaaaaactc gttttttatg attttttttc
    25981 tggtattttt ccttcaggcc accaaagttt gatcaaagct tgggttccgt cattctcaaa
    26041 acctttatct gccaactctt catatagttt caatgcttgg gtagttgctg gcaatacaag
    26101 atccattttt tctgcttctt ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa
    26161 aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa taattgctta aagaccagtt
    26221 agctgcactt ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc cgtttttttg
    26281 tgcatagaca agcatttccg tcattcctgt catcgtacct gcaatcatga tctgattagc
    26341 catttttgta tgctgacctt tcccagcaga accatgcaat gtaaatgtcg taccaaattc
    26401 tttgaataaa ggcagtactt gatcataaac ggcttcttct ccaccgacca tgatcgtcag
    26461 tgtaccattc tttgctccta agtcaccacc ggataccggt gcatctaacg catctgcgcc
    26521 tttttctttt gctgtttttg caattttttc tgccaggctt ggtgtacttg tagtcaaatc
    26581 aactaaaata tgtcctgaga tatctgcaga aaaaatcccc gactgtccat agtagatttc
    26641 ttctacatct ttggggtaac ctaccattgt aaagagaata ttactttgtt ctgctaccgc
    26701 ttttggtgta tctgcccaga tagctccttc tgttactaaa ggatcagctt ttgcttttgt
    26761 ccgattatag acagctactt cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc
    26821 catgacgcct gttccgataa aaccaagttt cattttttcg acctcctaaa aatcaaaaat
    26881 ccttgcttct atgctagtat acgctagaaa caaggatttt ttcataaaaa atgtttgaat
    26941 tttatctttt aaatgaattt aattttattt tgattcgttc aatcgagtct tcttcttctt
    27001 ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg ataaaattcg atcatagctt
    27061 cgccgaaatg tgtagatgaa atatcataaa gtttagcttc taaaactttt ggatctttag
    27121 gaacttcact ggaaatataa tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag
    27181 ttttccctaa tgaaggatga tcgaacaatc ggttcaaata agcatttcct tccacgacac
    27241 aaggtgttcc ggctgccatc gcttctgtat aagtaagccc ttgtgtttct gaagtagaag
    27301 cactgacaaa ataatctgcc gctttataat aaagtgctac atcattgttg tccacttctc
    27361 ctgtgaactg cacaaattct tctaactgca gttctgagat caattcctct aactcttcta
    27421 cataaggtcc ttttcctacg ataactaatc tggcgttagg ataagcggag atgacctgag
    27481 gaaacccatt aatcaaggct tggatatttt tctcataaga aatcctgctt aaagaaagaa
    27541 tcatcaaatg gttttcctga agacctagtg actcccttaa tttgctgata tcctgctgag
    27601 tgatatcaga acgtttgaat ttttcaatgt cgataccagt aggaatcacg cgaagcggtg
    27661 ctattacacc atagctgcgt aatgtatcaa taacacgttc acttggacaa actacacctg
    27721 tcgtatgatt tgcaaataat ctagacaagt attttacatg ggattgcctg atcacttttc
    27781 ctttaccgat ataatgaagg tagtcttcat acatcgtatg gtaagtatgg atcagaggta
    27841 ttttcaactt cttagctacc atcttcccaa gtatccctgc tccgaattct gtatgggtat
    27901 gaataagatc tagttctagt tctttggcaa ccaaataagc ataccacatg cctctaacaa
    27961 caactcttcg atccttgaaa gaaataaatg gtacacttgg cattcgaatc acatcttttt
    28021 ccaacttatc cgcattagga tcggtcgttg taaaaatata gacttcatgc ccatgtcttt
    28081 ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg
    28141 tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa actagtcctt gacctgattt
    28201 acttttttta atttgctaga gactttttca ttcagtacac tttcgtatac atttttaagt
    28261 tcttcaccga ttttattgat acttcgctct tttgctgttt gataaccggc ttgtgtcgta
    28321 ctcggtagtt cttgattggt aatctgttgg atcagtcgga taaattcttc gtttgtcttc
    28381 cccatatagc agttcttgct atcttctaac cagccattat acactggaat atctcgaaca
    28441 agtacgtttt gtttgcttgc taaagcttct aaaacaacga tcccttctgt ctcttcataa
    28501 gaagggaaaa agaatagatc tgcacctgaa tacgcacctt ctatgatctc tccacgaatg
    28561 taaccaggaa acaggacatt ttcaggatga tcttctttta caattttccg gatgttccga
    28621 ggaatcgagt acatgggaac atgaccaaac caaataaatg tgtattcggg catcttctta
    28681 gcaatttcta cgaaatcaat gattccttta cgttcgaaaa acaaaccgac acaaatgatt
    28741 actttttgat ccggagacag tttgaaatat tctctgaact tttgttcttt cattggatca
    28801 gggtgatatt tctctaagtc gatcccattg gaaatagctt gaatcggcac acggatacca
    28861 tagctttcca acaactgttt tgaataaggt gttggcgtga tcagatagtc cgctttttgg
    28921 tacaatccta ccagatattt ttttacgata ggagaaagct ggttcgatcc gataaaggaa
    28981 tttcggaaat cttcctctgt agaatgcgca tgatagacaa ctttttttcc ttctcttctg
    29041 gctttattga ccatattatg actattgatt ccgtatgtat tgatgtgcag gatgtcataa
    29101 tcttcttttt catctagtgt ataagagata ccgacttccg tcagagcacg tttttgatga
    29161 tcaagcgcac gcccgattcc agattttgca agcatctttt cactttcaaa atataataat
    29221 accttcacct tacgtccccc ttcacttata tagtataaca tagcctacaa actgtcactc
    29281 aaacttaagc cctatttttg tctattctta ctgacaaaaa cgttttctta aaaaagaaca
    29341 aaaaaactgg cgtagctctt ttacaagcca caccagcttt tgatacgttg tttattttac
    29401 atattcatga acaagttcaa cgacttcttc catcgtatcg cattctttca atgcacgatc
    29461 agcaagttca gccattttcg ctgtgtccaa gcgtttcatt aaactacgcg ttttaagaat
    29521 agatgtcgca ctcattgaga actcatctag acccatacct actagcaatg gaacagctgt
    29581 ttgatctcca gccatttctc cacacatacc tgcccatttt ccttcagcgt gtgctgcatc
    29641 aattacattt ttgatcaaac gtaaaattga tgggttatat ggttggtaca agtaagaaac
    29701 gcgttcgttc atacggtctg ctgccattgt gtattgaatc aaatcgttcg tacctacaga
    29761 gaagaaatct acttcttttg caaacttgtc tgctaagact gctgctgctg gaatctcgat
    29821 catgatgccg acttggatcg tatcagatac ttccttgcct tcacttatca atttttgttt
    29881 ttcttcttca aagatcgctt ttgctgcgcg gaattctttg agtgtagcta ccatagggaa
    29941 catgatacgt aaattaccat gaacagatgc acgaagcaat gcacgcattt gtgtacggaa
    30001 catttcgtcg ccttgttcag ataaactgat acgcaatgca cgatatccta agaatggatt
    30061 catttcatga ggtaattgca gatatggtag ttctttatct ccaccgatgt ccattgtacg
    30121 aacaactaca ggttttcctt ccataccttc taatacagct ttgtaagctt catattggtc
    30181 atcttctgtt gggaagtctg gagaatccat atacaggaat tcagtacggt acagaccaac
    30241 tgcttctcca ccattattat ggacaccaac tagatctttt ggtgttccga tgtttgcagc
    30301 taattcaaaa tgcttgccgt cagctgtgac agtttctgca ttttttaatt tttcccattc
    30361 tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat tcagcttttt gttcatctgt
    30421 aggatcgatg attacatcgc cttcgatccc gttgacagcc aaaatatcgc cttctttgac
    30481 tttagcagta atttcttttg tcccaacgat tgctggaatt tctaaagaac gtgccatgat
    30541 agcagaatgt gaagtacgtc caccaatgtc tgttacaaac gcttttacaa aattgcgatc
    30601 taattgtgct gtatcacttg gagtcaagtc atgtgcaaca acgaccactt cttcgttgat
    30661 cattgatgga tttggtaaag taacgcctaa taagtgcgct aaaatacgtt tagcaacgtc
    30721 acgaatatct gcagcacgct cttgcatata agcattatct tccattgctt cgaacatacc
    30781 gatatacata tccgttactt cttttagcgc tgattcagca tttacgctgt tgtctttaat
    30841 attttgcttg atttgaccta ccatttctgg atctgaaaga accatcaaat gggcatcaaa
    30901 tacttgcgct tctgcttcac ctaagctttg cgctgctttt tcacgaattt gctgcaattc
    30961 ttcagtagat ttagctaatg cagcatccaa acgagcttct tcagccgcag tatcttctac
    31021 tgttgcctta ctgaatgtta aatccggttg aactagcagg taagcttttg caacggctac
    31081 accgtcacta gcggcaatcc cttttagcat ttcaaccatt attcagataa gccttctttc
    31141 ttcattgttt caacgattgc tgccattgca tcagcttcgt cagcaccttc tgcagtaata
    31201 gtaacatcag aaccttgacc tacacctaaa gacataacgc ccatgattga tttcaagttt
    31261 actgatttac ctttatactc taagtttaca tcagagttaa atttgcttgc tgtttgtact
    31321 aatagtgtag ctggacgagc gtggatccca gtttctgcta ctacgtgaaa ttctttcttt
    31381 tccatattaa tcggtctcct ttaagtgagt gttgttttta tgtgagggtt ttctttttta
    31441 gtaattcaga gaatcctaac aaatgccacc ctttcatctg taaagattaa catttttttt
    31501 cgttagatac aacagttttc attcaatttc agataaaagt attcaaatta ttccggattc
    31561 tttttcatcc ttttcttcac attttataac aaacgtgttt atttcctttt tcttgtcgct
    31621 ttctcttctc ctgacgttta tcattctttc ctttgaccct ctttgttttc atatgaaacg
    31681 aattacttgc ttcccttttt atttttagta taataagagt caaaggtcag gaatggtcaa
    31741 tagagatttc accttttcta cctcataaat ctcttaaaaa actaggggtt attcttttac
    31801 ctcatgaaaa acgagatgta aaatatggat gtacccattt ttgtgaaagg acgtgaatga
    31861 ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga ttcacttgta tgccactgtc
    31921 aatggtcagc ggacacaact cgattactgt cagagctgtt atcaaaaaat caaaaatcaa
    31981 caaaatggag gtctgactaa tatggcccaa aatgatccat ttggatttgg cagtttggac
    32041 gatcttttcc gttccatgtc tcgccaaatg caacagcaag gaaattatga gcaaacacca
    32101 ccaacacaat ttggcggaaa caacaacttc aatggtggtc agccacctca aggcaataca
    32161 gaaggtttat tgggagaata cggaatcaat atcacagaac aagcacgaca aggtgatatc
    32221 gatcctgtga tcggacgcga tgacgaaatc aaacgagtca ttgaaatttt aaatcgccga
    32281 accaagaaca atcctgtctt gattggagaa ccaggtgttg gtaaaacagc agttgttgaa
    32341 ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa aattaatgga caaagaagtc
    32401 attcgtttag atgtcgtatc tttagtccaa ggtactggta tacgaggaca atttgaagaa
    32461 cgtatgcaaa aattgattga agagatccgt caagcagaaa atgtgatcct attcatagat
    32521 gaagttcacg aaatagtcgg tgctggttct gctggtgatg gaaatatgga tgctggtaat
    32581 attttaaaac cagctttagc tcgaggagaa ttgcaaatgg ttggtgctac taccttaaat
    32641 gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc gtatgcaacc tgtacgggta
    32701 gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag gtttgcaaaa acgttatgaa
    32761 gactatcatc atgtaaaata tactgatgag gccatcgaag cagccgcaac tttatccaat
    32821 cgttacgtcc aagatcgctt cttgccggat aaagccatcg atttgctaga tgaaacaggt
    32881 tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa caatcgagaa aaaactgcaa
    32941 gaagcagaag aacaaaaagt tttagcttct cgagaagaag atttcgaaaa agcagcttat
    33001 tatcgcgatc agatcaataa acttcaaaaa atgaaagaac gtcagctcac tgaagaggaa
    33061 acacctgtga ttaccgaaaa agatatggaa aaaatcgttg aacaacggac aggtattcca
    33121 gtgggtgaat taaaagaaaa agaacagaca caactgaaaa acttagcaga tgacttgaaa
    33181 gctcatgtca tcggacaaga taacgctgta gatcgagtag cgaaagcgat tcgccgaaac
    33241 cgtgttggat taaacaaaca gaatcgtcca atcggctcct tcttatttgt tggaccaact
    33301 ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg aactattcgg ttcacaagat
    33361 tcaatgattc gtttcgatat gtctgaatat atggaaaaac atagtgtctc taaattgatt
    33421 ggttctcctc caggttatgt cggatacgaa gaagctggtc aattaacaga aaaagtacgg
    33481 agaaatcctt acagtcttgt cttgttagat gaagtagaaa aagctcatcc agatgttttg
    33541 catatgttct tgcaaatctt agatgatggt cgattgacag atgcacaagg aagaacggtc
    33601 agctttaaag atacgatcat cattatgaca agtaatgccg gaactggtaa agtagaagca
    33661 aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt ctgtattgaa ccaattaaac
    33721 aactacttta caccagaatt tctgaaccgt tttgacggga tcatcgaatt cagtgcatta
    33781 tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag acgatgtcaa ccaaatgctg
    33841 gcagcacagc aacttcacat cgaagttcct acgaatgtca aagaaaaatt agttgactta
    33901 ggctatgatc catctatggg tgctcgtcct cttcgacgca cgatccaaga acaaatcgaa
    33961 gatggtatcg ctgaatttta tcttgatcac ccttctatcc atgagttgaa agcaaaactg
    34021 gataaagatg gaaaaatcat cgttacttct aaaccagaac gtttggctaa agaatcagcc
    34081 gaagaaacgg ctgaataaat ccagttttat agcgtgagac ggtatttctt tgagaaatgc
    34141 cgtctcattc ttttttcaga aaagtcaaaa tactgttcat tttataaaaa agaaattata
    34201 ataagagaaa caagcaatta aaaatacata aaagaggtga gcgccatgaa attggtaaat
    34261 gtaacaaaca gccattcccg tcttgtatta aaccagcttg aaaacactga tgcccatcta
    34321 gtcaaggtct acacagccgg aaataccact attgtctaca ctgaagcccc tcaacataat
    34381 gaaattctgc tgatcaatga caaacgtaaa atccagccga aagaaatcga agatgctaaa
    34441 aattatttct tagataaaat caaagacgcc gtttaccacg aggatgaaat caaagtaatc
    34501 gaattggcag gccttgttga aatctcgatt ccaaaacatg aattaggcgc tacattagcc
    34561 gtgtaaacaa attttgaaca taataattaa agagcgtggg acaaaagtaa a
//
LOCUS       DAZKCC010000021        33820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000021 DAZKCC010000000
VERSION     DAZKCC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.021"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..1455
                     /locus_tag="ACG8VH_001111"
     CDS             136..1455
                     /locus_tag="ACG8VH_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFP9109685.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(1658..2116)
                     /locus_tag="ACG8VH_001112"
                     /pseudo
     CDS             complement(1658..2116)
                     /locus_tag="ACG8VH_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            complement(2287..2733)
                     /gene="dtd"
                     /locus_tag="ACG8VH_001113"
     CDS             complement(2287..2733)
                     /gene="dtd"
                     /locus_tag="ACG8VH_001113"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9109686.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(2744..4957)
                     /locus_tag="ACG8VH_001114"
     CDS             complement(2744..4957)
                     /locus_tag="ACG8VH_001114"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9109687.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(5190..5954)
                     /locus_tag="ACG8VH_001115"
     CDS             complement(5190..5954)
                     /locus_tag="ACG8VH_001115"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9109688.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(5955..6902)
                     /gene="prmA"
                     /locus_tag="ACG8VH_001116"
     CDS             complement(5955..6902)
                     /gene="prmA"
                     /locus_tag="ACG8VH_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9109689.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(6915..7403)
                     /locus_tag="ACG8VH_001117"
     CDS             complement(6915..7403)
                     /locus_tag="ACG8VH_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP9109690.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(7369..8040)
                     /locus_tag="ACG8VH_001118"
     CDS             complement(7369..8040)
                     /locus_tag="ACG8VH_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP9109691.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            8178..9464
                     /locus_tag="ACG8VH_001119"
     CDS             8178..9464
                     /locus_tag="ACG8VH_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP9109692.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            9536..9730
                     /gene="ssrS"
                     /locus_tag="ACG8VH_001120"
     ncRNA           9536..9730
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8VH_001120"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            9808..10092
                     /locus_tag="ACG8VH_001121"
     CDS             9808..10092
                     /locus_tag="ACG8VH_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109693.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            10165..10644
                     /locus_tag="ACG8VH_001122"
     CDS             10165..10644
                     /locus_tag="ACG8VH_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9109694.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            10867..11010
                     /locus_tag="ACG8VH_001123"
     CDS             10867..11010
                     /locus_tag="ACG8VH_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9109695.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            11355..11483
                     /locus_tag="ACG8VH_001124"
     CDS             11355..11483
                     /locus_tag="ACG8VH_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109696.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            11588..13171
                     /gene="abc-f"
                     /locus_tag="ACG8VH_001125"
     CDS             11588..13171
                     /gene="abc-f"
                     /locus_tag="ACG8VH_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP9109697.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(13608..14792)
                     /locus_tag="ACG8VH_001126"
     CDS             complement(13608..14792)
                     /locus_tag="ACG8VH_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP9109698.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14817..15824)
                     /locus_tag="ACG8VH_001127"
     CDS             complement(14817..15824)
                     /locus_tag="ACG8VH_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9109699.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(15996..16355)
                     /gene="comGG"
                     /locus_tag="ACG8VH_001128"
     CDS             complement(15996..16355)
                     /gene="comGG"
                     /locus_tag="ACG8VH_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP9109700.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(16327..16752)
                     /gene="comGF"
                     /locus_tag="ACG8VH_001129"
     CDS             complement(16327..16752)
                     /gene="comGF"
                     /locus_tag="ACG8VH_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP9109701.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(16715..17062)
                     /locus_tag="ACG8VH_001130"
     CDS             complement(16715..17062)
                     /locus_tag="ACG8VH_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109702.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(17034..17504)
                     /gene="comGD"
                     /locus_tag="ACG8VH_001131"
     CDS             complement(17034..17504)
                     /gene="comGD"
                     /locus_tag="ACG8VH_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP9109703.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(17479..17757)
                     /gene="comGC"
                     /locus_tag="ACG8VH_001132"
     CDS             complement(17479..17757)
                     /gene="comGC"
                     /locus_tag="ACG8VH_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP9109704.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(17754..18803)
                     /gene="comGB"
                     /locus_tag="ACG8VH_001133"
     CDS             complement(17754..18803)
                     /gene="comGB"
                     /locus_tag="ACG8VH_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP9109705.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(18760..19689)
                     /gene="comGA"
                     /locus_tag="ACG8VH_001134"
     CDS             complement(18760..19689)
                     /gene="comGA"
                     /locus_tag="ACG8VH_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP9109706.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            19821..21575
                     /gene="ade"
                     /locus_tag="ACG8VH_001135"
     CDS             19821..21575
                     /gene="ade"
                     /locus_tag="ACG8VH_001135"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9109707.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(21673..22335)
                     /gene="trmB"
                     /locus_tag="ACG8VH_001136"
     CDS             complement(21673..22335)
                     /gene="trmB"
                     /locus_tag="ACG8VH_001136"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9109708.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(22405..23187)
                     /locus_tag="ACG8VH_001137"
     CDS             complement(22405..23187)
                     /locus_tag="ACG8VH_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP9109709.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(23391..24602)
                     /locus_tag="ACG8VH_001138"
     CDS             complement(23391..24602)
                     /locus_tag="ACG8VH_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109710.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(24595..25332)
                     /locus_tag="ACG8VH_001139"
     CDS             complement(24595..25332)
                     /locus_tag="ACG8VH_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109711.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            25470..25904
                     /locus_tag="ACG8VH_001140"
     CDS             25470..25904
                     /locus_tag="ACG8VH_001140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP9109712.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            25897..26241
                     /locus_tag="ACG8VH_001141"
     CDS             25897..26241
                     /locus_tag="ACG8VH_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9109713.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            26421..27431
                     /locus_tag="ACG8VH_001142"
     CDS             26421..27431
                     /locus_tag="ACG8VH_001142"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9109714.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(27500..28444)
                     /locus_tag="ACG8VH_001143"
     CDS             complement(27500..28444)
                     /locus_tag="ACG8VH_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9109715.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(28428..31148)
                     /locus_tag="ACG8VH_001144"
     CDS             complement(28428..31148)
                     /locus_tag="ACG8VH_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9109716.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(31145..32365)
                     /locus_tag="ACG8VH_001145"
     CDS             complement(31145..32365)
                     /locus_tag="ACG8VH_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9109717.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(32646..33491)
                     /locus_tag="ACG8VH_001146"
     CDS             complement(32646..33491)
                     /locus_tag="ACG8VH_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9109718.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        10113 a         6927 c         5872 g        10908 t
ORIGIN      
        1 aattaaaacc atgtgaaact gcctcacggc agcttacaat tactttttca tcttcacccg
       61 ttaagtgatg aaaaaagaac ccctttctgc tatggttaaa gtgactaaac caaccaaaag
      121 aaaggagttc ttttcatgac tagcttacac aaaaaccaag taaaattcaa ttcaaatatc
      181 actatttctc atacaggtgg tcaattatcg agtgattcgg gtctcgtcct agtgaaagag
      241 ctgatgaaca tctttggttt ttctgaactg gctaagcaac acattcacat tgaagacgaa
      301 cgagcgtatt ttactcatga caacttatcc atacttgagc agttcattat gcaattaatt
      361 gctggttact cagctgattc tgcagctaat ctcctgagac aagatcctgt gtttaaagct
      421 gttttagata ggaaagaact cgcttctcaa tcttcacttt cccgattttt agatcgacta
      481 tctgaggaga atatccatga acttcaagct ttgaaccaag aacttatcga taaagcacgc
      541 ctcatccgta atgatacgga attaatcatt gatttagatt cgacccattc agatacattt
      601 ggtcaccaag aacaaacgaa ttataataca cactaccaaa cctatggtta ccatccattg
      661 gtagcttttg acggattgac tggtgacttc ttaaaagctg aactacgttc aggaaatcaa
      721 tatacatcaa aaggcgtaaa ggagtttctc acacctttat tagagcacta taatcactct
      781 ctaccaaaca ctgacatctt ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat
      841 gattcgtgtg aatcaaaaaa gagtcattat gttattcgac tgaagaataa tcgtagacta
      901 ggtcaaatag ctgagaaatc agtactttat ggcgataatc aaaagtggga agaacgagaa
      961 gttcagtact tctcgaccac ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt
     1021 atacgctcaa cacgtgaagc gggcgaacta ctctttcgac atgaatttat cgtgacgaat
     1081 ctatcagaaa atgtttctcc tgataccatc ttttctatct atgccaaacg tggcacaatg
     1141 gagaacttca ttaaagaagc gaaagctggc ttttactttg acaaaacaga cagtcctcgc
     1201 tttttggaga atcatgtccg aatgatgctc agcttgatag cttacaactt agtcaacttc
     1261 ttaagaacga ttggctttga agaagtccaa aagggaatga ccattcattc tattcgattg
     1321 aagtttctga aagttgctgg gaaattagtc caaacgggta gacgagtcta tctcaaatta
     1381 tctagttatc atgtgtatca gaatgaattc tacagggtct ttgctcgcct gaggcgagcc
     1441 agtcaatgga tctaatccaa caatctaacg atttttttac tgggaagtta tccagggaga
     1501 agtatgctca aaatgcctta cacccaaaat ttattcctca atatttttga acagtgaatg
     1561 tttgaagtaa ctttttagtc aaaatcagtt gatagggata ttagtgatac atatttcaat
     1621 aaaatgtgcc aaagaattaa agctatgaat tattcaggga taaattgata cctgcccact
     1681 cgcaaataga tgtaaagaaa accatcttac ttaaatcaaa tagttcaaat tgaccatgct
     1741 gtgcttcaag tgcttgtgtc agattcttat ctaagatacg atgataatct tccattcttt
     1801 ccggtgtcat tagatccata aaaatatttt tccgatggtg atgagcagct ccatccaaag
     1861 tctgtactcc accttgacca aaaagtgtct tcaatactaa ttttggcata gcaccttttc
     1921 gtttgaaatt ccttggatca taaaatactt ttgctgctga tgatccgtag atcgtgatag
     1981 cttctttatt gaaaatcttt gctttgacga cgggagcgtc cgcttcatgg cgtaactcct
     2041 ccaacatgtt gtatcctttt tgatacaatt tttttaaatc tgtgattttg atatctacta
     2101 ctggtacttc tttcatcttc agcaacctcc tataaaaaat aacttgtata atttaagctt
     2161 aacttttttt ccgactgctg acaaaagaag cgctttttgc agacaaaaaa gccacttaag
     2221 ttagagttta tcagtctaac ttaagcgacg tgtttcatag tttttactag tgaaaaccag
     2281 attactttat ttgtttttag aatcaataat gatcgttaca ggtccatcat tgacgagaga
     2341 aaccgacata tctgctccga attgtccggt agcgactgtg atgccgcgac ttctgattcc
     2401 ttcattgaat gattcgtata aaggaatagc tgtttctggt cgtgcggctt ccacaaagct
     2461 tggtcgattt ccttttttcg tatccgcaaa cagtgtgaat tgagagatac ttaaaatttg
     2521 tcctcccaca tcttctacac taatattcat ttttccttgt tcatcttcaa aaatacgcat
     2581 ttgtgcaatt ttttttacca gatagtccac atctttttgc gtatcggttt catgaatgcc
     2641 taataaaaca agtaggccgt agccaatttt tccaatttct tgctgatcga ctgacacagc
     2701 agctttactt acccgttgaa caactgctct catatctgtc ctcctatcca ttcgtccggc
     2761 gtacactgta gacatcaggt actgctttga ttttatccac gatactcttt aggtgggata
     2821 aattctggat accaaccgtc aaatggatcg tcgccatttt atctttattc gtccgtgctt
     2881 caacgctgtt gagatttttc gttagcgcat tgactgtttg aagtacatca ttcaataagc
     2941 ctgaacgatt atagccgtaa atttccaaat ctgcatcata ctcttttcgt gtattcgatg
     3001 tatcttccca ttcgacttca atcaaacgtt ctgctacatt tggtttgtca ggctgaacat
     3061 tcggacaatc tcgacgatga atggatatcc ctctgccttt agtgatataa ccaacgatat
     3121 catcaccagg aatcggatta cagcagcgac tgatacgaat tagtaagttt tctacccctt
     3181 gaatgacaac gccaccttca tgacgtactt tcattttgtc aggttctttt ttcggctgat
     3241 tcatgatttc ttctgcttct tgcttttgct gctcgatttt ttgttcttta cgttcttttt
     3301 cagtcagacg attggccatc gtcaaggggc taacttctcc ataccctaca gctgcataga
     3361 gatcatcttc tgtttgataa ttaaaacgat cgagtgcttc ttgcagtttg ttcttcgtca
     3421 aaatatcttt aggcgtaaat cctagatctg taatacattt gaccacggat tcgtggcctt
     3481 tgatcacatt ttcttctcga tcttgggctt tgaagaaacg tttgatctta tttcttgctt
     3541 tgctagtagc aactaatttc aaccagtcgc gacttgggcc aaatgaattt ggagaagtca
     3601 tgatctcaat gatatctccg tttttcaatt tgtaatcaag ctgcaccatt ttgccattta
     3661 cttttgcacc agtggttttg ttaccgatat ctgtatggat actgtatgca aagtccaatg
     3721 gtccggatcc ttttggtaac tcagtaacgt ctccttttgg tgtgaagacg tagactttat
     3781 cactaaagat atctcctttt acgccttcca taaattcaga agcatcatag ctttcgtctt
     3841 gaagttcgag tatctcatgg aaccagctta attgtttcgt cataccatct ggttctactt
     3901 tttcattttt tccttcttta taggcccagt gtgcagcaac cccgaattca gcgatttcat
     3961 gcatttcctg tgttcggatt tgaatttcta ccggatttcc tgccggaccg atcacggtag
     4021 tatgcaaaga ttggtacata ttagcttttg gcatggcaat ataatcctta aatctgccgg
     4081 gcattggttt ccattttgtg tggattgctc ctaatacagc ataacaatcc ttgattgaat
     4141 ctacgatcac tcgaatagct agcaagtcat aaatttcgtt gaactgcttt ttctgatctt
     4201 tcattttacg atagattgaa taaatgtgtt ttggccgtcc atagatttct gcaaaaatcc
     4261 ccagctcttc tgtcgctatt cgaatatctt caacagtacc acttacgtat ttttcacgtt
     4321 cttctctctt cgtctgcatc aagtggacga tacggtaata ctgttttgga tttagataac
     4381 gaagtgctgt atcttctaat tcccatttga tccggctgat ccctagacga tgtgcaagag
     4441 gcgcatagat ttctaaagtt tcctgagcga tccttctttg cttatcttct cgcaggtgct
     4501 tcaacgtacg catgttatgt agtctatcag ctaatttgac catgataact cgtaaatctt
     4561 gagccattgc tagcagcatc tttcggtggt tttctgctag ctgctcttcg tgggatttat
     4621 attttatttt ccctaatttg gttacgccgt ccactaacat agcaacgtca tcgccaaatt
     4681 cttctctcag atcttctaaa gtgatttctg tatcttcaac aacatcatgt aagaagcctg
     4741 tagccactgt atggggatcc atttttaaat ctgctaatat acctgcgact tggataggat
     4801 gaataatgta gggttcgcct gattttctaa actgtccgtc atgtgcagca gtcgcgtatt
     4861 cgcatgcttt ctcaacaaat gctacatgtt cgggacccat gtattgacta acgagcttta
     4921 tgacaccggg tcccgttaaa atttcttctt ttgtcacagg aatcgatccc cctttctttt
     4981 ttcggtaaaa cgggaatcgg gacaaatatc tgtttgataa ttgtcccaaa atccgtgcag
     5041 ttatacgttt ttttacggaa atgttcgctt tttaaaagct ttttcaaacg atttttcgta
     5101 tccattgtat aaataattgt ttctattata caaattgttg actatttttt caattctttc
     5161 tgtcgatact cttttattaa tgaagaacgt tagctgaaag aaggattcat taattcccat
     5221 tgatagctag cagcacttaa taaataaaga ggtgctgttt ctgtacgaag gattcttggt
     5281 cctaatccac aaagtatccc tttgtgttct acaaaataat cgacttcatc gcgggataat
     5341 ccaccttctg gtccaaagac agctaagatt cgactatctg gttttgtttc actaagaatt
     5401 ttggctgcat ttgatttttc gccttgcttt gctgattcct cataagcaac caagaccacg
     5461 tcataatcat ctagccgctc aaataaatct tgtttcgttt ccaataactc tactgatggc
     5521 acgatctgcc gatgggattg ttccgctgcc tcttgagcaa tcttttcaag ccgttgttgt
     5581 cgtttcatac gtttttttgc atcccattta acgacagagg ctgatgccgg gaaaccaaga
     5641 aaagcttgcg ctcctaattc agtggatttc tgaacgatcc aatccagctt ttccccttta
     5701 ggatagccac tcgctatggt aatcgaactt ggcagttcct tttgattttc ttctttttta
     5761 atttctttca gataaacagc ctcttcagta atctccgtga tttctgcaac aatggctatt
     5821 tgatcattga atgccaaaaa aacttcatcc tcgggttcca tcctcataac ccgtacaatg
     5881 tggtgatagt tttccccttc taaaacaaaa tagtcttttg attcatatga ttcttttaaa
     5941 aaataccgct gcatctattc cacctctggt tttttcaaaa taattgcata ccagtctttt
     6001 tgctggaaaa tttgatccac gataaaccct tgttctaaca tggcatccag aaccatttgc
     6061 ttcttctctt caatgattcc tgaaacgatc agcgtgcctg tttgcttcag taaacgccaa
     6121 gcatctggga tcatcaataa gatgatatct gctaaaatgt tcgcaacgat tacatcactt
     6181 tcaatctcga ttcctgtcaa aagatcgtta gctgatacat ggacatcttt ggctatttca
     6241 ttcatatcca tattttcttt cgccgaacga acggctactt cgtctaaatc aaaggcatag
     6301 acatcttttg caccaagata ttttgctgca atacttagca cgcccgaccc agttcccaca
     6361 tctagcaatg tttcaccacc acgcaagact gtttctagtg ctgtcaatgt caaggaagtc
     6421 gtgggatgag tgcctgtgcc aaatgccata ccaggatcta gtgtgataat tttttctaga
     6481 ggatcttctg tctggtagtt ttcccagctt ggcgcaaccg tcagcattcg cgtaatacgc
     6541 accggatgat agtatttctt ccaggctgtt gcccagttgc tttcctctac ttcactaact
     6601 gttactttat tttttccaat agctagacca aactcaggta attgtgtgat acgatctttg
     6661 ataaaaggca agatttccgg taagaaagtc gtttccggaa aataagccat gactagtgcg
     6721 ccttctttca gcgatgtaaa atcattttta tctaaaatct caccatattg atccgactga
     6781 aaatgttcaa catctaaaga atcttcaatc gcgacaccac tcgcccctgc ctccatcaaa
     6841 atattagcaa ctgcttcgac tgcttcactt tcagtttcta cttttacttc attccatttc
     6901 atatctgtca ctccttaata acttgggtac ggtaaataag tatctgcttc attttgctgt
     6961 tttacttcta ctaattctgc tagttgctga ttatcccagt gaagctcgaa tacagcttcg
     7021 tcatcgtctt catccaaaaa atcaagcaaa tcgctttgcc cttgagctaa tacttcattt
     7081 agatattcca ctaaagaatc agctactgat tttgccaacc ctttttttcc ttcataaggg
     7141 attactgcta gataattgcg ttcatcaaaa acagatttct gaggattaaa aagaagtacg
     7201 ccgtcttcaa attcgataat tggttcttgc gaaacgatcc cttctgcatc atcaatctca
     7261 aattgtgctt tattttctgc aaacaagcga acaacgatct cgatcgtatg attttttgta
     7321 tcccaatcta aagctacatc atagtctgtc acttgttttt caattatttc atctaaatat
     7381 gttaatagat ccttcttttt cattttcttt cctccaaccc caatctttct cgatttggtt
     7441 ttttctttgt aaagagatat aaggatttcc cgacacaaca aaacgcaacg gcttttctgt
     7501 ccatacacct ttgtttggga taccgattct aggcaaagca ttgatctttt ttggtgtctt
     7561 ttttttctca aaaactaagc gtaacgaact atctgctata gactgaccat aaagttcttg
     7621 aggaattgct aatgcttcca ctaattttcc cggaccgttg ctgatatctg gaccagtttt
     7681 tccgccacga tttttactca tttgatcaat cattgcggcg ggttcgattg cacgtatcat
     7741 tactccttga ggaatccctt ctggctgagt aatgatgttc aaaatccgat gagtatgcat
     7801 cgtgtaaagg tagatcgtac ctggtttttc atacattgcc cgcacacgcg gtgttcttct
     7861 taaaccatag ctatgggcgg cctcgtcttc tggacctagg tatgcttccg cgtctacaat
     7921 atatccagct aatcttcctt ctggtgtgtc atgctccaaa tacatcccca ataaatcccg
     7981 tgccacttct ggcgtcgttt tattccaaaa aaattcatct atcttttgtt tatccgtcat
     8041 ttttctcacc tactttatca tacacaatat tagttggata agctatgaaa acaaaacaac
     8101 agaaggctgt tctttctagg catcttgcta aatatgatat aattaaggga cgtaaaaaca
     8161 agaaggagga aaatcctatg caacagccat tggcttatcg catgagacct cgaacgatcg
     8221 atgaagtcgt tggtcagcag catctagtcg gggaaggaaa aatcatacgc cgcatggtcg
     8281 atgctcgcat gctctcttcc atgattctat atggacctcc tggtactgga aaaacgagta
     8341 tcgcaagtgc gatcgccggc tctactaatt atgcatttcg catgctaaac gccgctacag
     8401 attcaaaaaa ggatctacaa gttgtagcgg aagaagcaaa aatgagtggt acagtgatcc
     8461 tcttattgga tgaagtacac cgattagata aaacaaaaca ggattttctg cttcctcatt
     8521 tagaaagtgg ccgaatcatc ttgatcggag cgactactga gaatccttat attaccatca
     8581 atccagcgat ccgcagccgt acacagatct ttgaagtcaa accattaaca gaacaagaca
     8641 tccagttggc agtcgaacat gctttaaggg ataaagaacg cggccttgga caacaagcaa
     8701 tccagttaga cgaagatgct cttcttcacc tttcgcgtgc aacgaacggc gatttgcgga
     8761 gtgcattgaa tgggctggaa ctggctacac tatcgactcc ttcagataaa gaaggaagga
     8821 ttcatcttac actgtcgatc attgaagaat gtgtccaaag aaaagctctt acccatgaca
     8881 aaaatggaga tgctcattat gacgttattt cggcttttca aaaatcgatt cgtggaagtg
     8941 atgtggatgc tgcacttcat tatctcgccc gtttagttga ggcaggtgat ctggcaagta
     9001 tttgccggcg tctgatggtt atcggttatg aagatatagg tttaggtaat cctgctgctg
     9061 cggctcgtac agtgaacgct gtattagctg ctgaaaaatt gggattgcct gaggcacgta
     9121 tcccactagc agacgtcgtc gtcgatcttt gcttgtcgcc aaaatccaat tctgcctaca
     9181 tggcattaga tgcagcactt gctgatattc gagagggcaa agctggagat gttcctgatc
     9241 atttgagaga tagccattac aaaggtgcga aatcactcaa ccgtggcgtg ggttatcagt
     9301 atcctcatca ttttgatcag gcttgggtca atcagcaata tttaccagat aaactgaaga
     9361 atgctcagta ttatcagcca aaagatacag ggaaatatga acaggctctg ggtcaacagt
     9421 attaccgtat aaaagagtgg aaaaagcatc ctccaaaaaa ctagacaaga aaacggttct
     9481 gtttctgcga gctctctcat tgctcttttt aaattcatat gctactatag aggtgggaaa
     9541 tctgtgatgt tcgaattgtc ctctttgtgt gttgaccgaa catttttatt gatatcttgg
     9601 gacccgtctt gtgtcactgc tggtaacatg ccttatcatt tggtagttcg aagctacgac
     9661 catgggaccc acctgcttga attcgcggga tcaatactac aggacaaaca acggcattaa
     9721 cggagattac catcttttaa agaataaagc aatttgctct tggcaaattg ctttttctat
     9781 ttgttgctta ttttggagga tttgttcatg attaggattt taatgattat cgccacttta
     9841 gtattattat tcgttagcta ttatttattt aataaacagg atatcttttt tgtattgatc
     9901 aacaaaaatg ataaaaatca aggattccta caattttacg gagctgcata tgctgtttta
     9961 ggcgtaatgg gtattttaac agcttttttc aatcaacgct ttattgcctt ggtcttttta
    10021 ctgatcgtaa ttattgtttc tgccaccttc agtatccgat ttgcaaaaaa aattgctgaa
    10081 ccaaagcagt aaataattct ctttttccct aaagtgttct acaataaaag agtacaatac
    10141 gattgacttg aggagtgaat gcttatgtta caacaataca aaaaaatcat ggttgccgta
    10201 gatggttctg atgaagctga attagctttt aaaaaggcgg tcaatgtagc tatccgaaat
    10261 aacggagaac ttctattagc acatgtcatt gacacacgtt ctttccaaac cgtttcatcc
    10321 tttgatggaa tgcttgcaga acaagctact gaaatggcaa agcaaacatt ggcagattat
    10381 gaaagcaatg cgaagaaagc tggcttgaat aatgtaacct ctgtcgtaga atatggctca
    10441 ccaaaacaaa ttattgccag agaaatccca gaagataatc aagtagattt gattatgtta
    10501 ggagccacag gattgaatgc tgtagaacgt ttatttattg gttctgtttc tgaatatgtt
    10561 attagaaacg cagcttgcga tgtgcttgtt gtccgaactg atttagaaaa ccaattgcca
    10621 gctaaagacc acaccaaagc ttaacacgta gacgacctgc caactatcgg caggtttttt
    10681 tgcacaaaaa accgtgataa aaacaaaaga cgcggcaagc actcatcggt ctttataatg
    10741 tttaaaataa agggaaaaca gtcaatgagt tcaagtaaaa taaaaaaaag tgaacaccag
    10801 ggcgattagt cgccctggtg tggtttaaaa cgacctaagc atgccaaagc ttattggttc
    10861 gtcaatgtgg aagtttccga tgaatctgat acgaaaaaat atgaagtatc gatggcggat
    10921 accgatttta tcgccaaact aaaattttat ttcgggttcg ggtaccgcga gaaggcaagt
    10981 gctacattcg gtaaatcatt caagaattga tgaaaggcta ccgcttgcca ttgcaaccac
    11041 aacgatactc ctgaacgcga tgttggtgag atttccaaag agaagagctg tttaatgcaa
    11101 cggtattaag caaatggcaa aaggaagtca tgtagacaaa gttttttatc aaacaccata
    11161 tgatttcgcc tgaacgctgg tttagctcag aatacaatga tgtggtccac gagacattac
    11221 cctcagtcag ataaaaaaag ctctctgaca gaattacaca tcgataaata ttaagtaaat
    11281 gtcagcgcta gttgaacttt ctgacacttt caggtacact ggaaaagaca actacacgaa
    11341 ggagggaaaa tactatgatg cgcatgacta aaataacaag taatgaaaat taccaacgat
    11401 accatatcgc atttagccat gcaattggtt ttacagttgc tgttgacggg acaagtttgt
    11461 ccaaaacagt tgaattcacg taaaatcaat ggccatctca aggtattttt ctcatgataa
    11521 tctgagttta aataacacag gccatggata accatggcct ttttgagtgg tctcaaggag
    11581 agagatcatg atcatacaag caatttctat taaaaaaaca atcggcggga atccattatt
    11641 tgaggattta tcctgtcaaa ttgaagctgg tgaaaaaatt gccttagtcg gtatcaacgg
    11701 aactggcaaa tcaacctttt tacaaatctt gaccggcaga gaaggtgtcg acagcggtgt
    11761 tatcagtcgc aaaaaaggct taaagatcgg gacggtcgaa caagaactta cagtttccga
    11821 agcgaccgtc aaccactact tgctccattc tgccgctgag atacaggacc tcaaacagca
    11881 aatgagtcgc tacgaagcat tgatgacaga accagataca aacttagaga agtgtctggc
    11941 ccgttacggt gagctgcagc atcgctttga agaactaggc ggttacgtat tagaagaccg
    12001 gattgcagcg atcttgcaag gcttagggat tccacaccgt caggaagcac ggttaacaga
    12061 acttagcgga ggcgaacgag ttaaagtcgc tttagccaaa attttagcag ccgatgccga
    12121 tttactttta ctcgatgaac cgaccaatca tttagatctt aacagtattc gctggttgga
    12181 aaactattta cagaatacaa aaaaatcggt tctggtcgtt tctcatgatc ggcaatttct
    12241 cgatcaagtc accacaaaaa catgggaact ggaagaaggt gcattgattg agtatcccgg
    12301 aaactacagc cgttttcgag tcttgaaaga agctcgcttg gctgagctga caaaaaatta
    12361 cgaactgcag caaaaagaag tccagcggtt gaaagtcatg attcgtcgtt ttcgtcagtg
    12421 ggcacatgaa ggagacaatg agtccttctt caaaaaagcc aaagaactgg aacgccgcct
    12481 ggcgaaactg acactcgtca aaccgccacc tcccccgaaa aatcggctgc aaagcttgtc
    12541 taatggtgga aaatctggca aagaagtttt catcattcaa aacttgcacc agcagtacgc
    12601 tgatcaagta ctattcaaag acagcagttt tgcagtctat cgtggagatc atttagccat
    12661 catcggcgac aatggtgcag gaaaatcaac cttactcaaa ttattactcg gtgaggaaaa
    12721 gccttacggt ggcaccatca aattaggtag tagcttgcaa atcggttatt tgccgcagca
    12781 gctgcagttt gccgatcccg atagccgttt gctggcttac gccatcacat tgactggaaa
    12841 cgaagaagtc gctcgacggc aattagccaa aagtggcttt tatcaaagcg atgtgggtaa
    12901 acggatcaaa gatttatccg gtggtgaaaa aatccgtttg gcgttgctga aactcttttt
    12961 agaaaaaatc aacgtcctga ttttggatga accaaccaat cacctcgaca gctatgctcg
    13021 agaagaaatt gaagaaatgc tgcaagacta tcaaggaacg ctcctagcag tcagccatga
    13081 ccgctacttt ttgcagcaac acttccaaga agcactcgtt attgatcagg agcagatcag
    13141 ccgccagccg ctcggccaac tgattgacta ggacgttttt tcgcaacaaa aaacttgatt
    13201 ttcatacctg tgctttcacg attcaggcaa agtacaggtg gaaaatcaag tttttattat
    13261 tcgatcaatg aatgtttcgg atcggttttc tagctaaaca taaagcgaag aataaagtcg
    13321 acagcccacg aggctgatct aaaaatgttt tttcttttta catatgtaat gtcccttata
    13381 aagatgaatg aaaataaaat attgggattt tcctgaaaat caataaataa aatgccagtc
    13441 ttttcccttt tgaccacttt tccaagcaaa attccctaag aagatcctgt aaaaaagaag
    13501 ggaaattgaa gttaaattac cattttgcgg tcttctccgc aaaaaaaaca catgcaagaa
    13561 ttccctcttg catgcgtttc atcttttatc ctaacgaatg ctttggctta gccgcgcaga
    13621 cgctccacat cacgggcaat cattagttcc tcatctgttg ggatcactaa tactcttact
    13681 tttgattctt ttgtagagat atctacttct tcaccacgca attcattcaa cgctggatcg
    13741 atttcacaac caaaccaaga catacctttg atgatttcac tacgtacatg tccgtcattc
    13801 tcgccgatac cagcagtaaa gacaattgca tcaacaccat tcatcgttgt cacataaccg
    13861 ccgatgtatt ttcggatgcg gtccacgaaa atgtcataag ctaaacgaat atgttctttt
    13921 tcatagttct tttcaagatc acgcatatca ctagacaatc ctgttaggcc tagcagacca
    13981 gatttcttgt tcaaaacatt gatcatttca tcaatatcaa tctctaattt ttccattaaa
    14041 tatgggagaa cagctggatc gatatcacct gaacgtgtac ccattgtcac cccagctaat
    14101 ggtgtgaatc ccatagaagt atccatggat ttacctccat cgactgctgt gatcgataca
    14161 ccatttccta agtggcaagt gatgattttt agttcttcga tcggtttgtt caataatttt
    14221 gccgcacgtt ccgacacata acgatgactt gtgccatgga agccatattt tcttactttg
    14281 aaatcttcat aataagacac cggtaagcta tataaataat tttgctttgg cattgttgag
    14341 tggaaagatg tatcgaatac agccacactc aaaatgtcag gtaaaatctt tttaaacgct
    14401 ttgatcccca ttaagtttgc aggattatgc aaaggagcaa attctgctaa ttcagcaatg
    14461 cggtccatta ctttctcatc gatcactact gaatccccat aagtttctcc tccgtgaaca
    14521 acacggtgcc ctactccggt gatttcatca taagagctta aaatttgcag ttcaatcaac
    14581 tgatctaaca gcatttttac tgctacttcg tggttttcga tatccatgat ttgttcgaat
    14641 ttttgattat cgccgtactt gatggtaaaa atagaatctt gtaaaccgat acgttcgacg
    14701 atccctttcg caactacggt ttcttctggc atttgatata attgccattt caaacttgaa
    14761 ctcccagcat tgattgcaat tgttttagac atatgactct ccttctcgtt gaatgattac
    14821 agagcagatg atttccagtt tttaaactcg ttgaaaaatt ccgtcacctt ctctggttct
    14881 tttaatgaag caagtttcac aagcaagact tcttttactt gttgtgcttg tggtcctttt
    14941 ttctgtaaga tcaaaatact tttttgcgat tgtttattac ggaacaattc atctggcaat
    15001 tgaatcattc cttgaagata accttcttct ttaaaccatt ttttgatttc ttcactttga
    15061 tccgtttcta aaaatccact cggcatcaaa aacaaaccaa aaccatccgg cttcacatat
    15121 ttcattgatt gctctagcaa taaatgatgt gcataactat ggccttcttc ggtacttgtt
    15181 aaaaactctt ttgctttttc gtcattcgga taataaccga ttggcaaatc gctgattgca
    15241 aaatcgacag gatctattaa taattcctgc aacccgtctt gatgaaagta ttgaacattt
    15301 gcttgtgtca aatcacttgt agaagctgcg acagccaaca atgtatcatc aatatccaca
    15361 ccaattcctt ctgtttggta ccccgcattc gataggttta aaagaactgt taataacaag
    15421 ttgcccattc ctgccgcaat atccagaatc ttaaccggtg cttttttatt tgtgtatagt
    15481 tgttcaataa gaaaaacaaa taaaaaaccg atactatcag gagtgagttg atggttcgcc
    15541 tgcaaatgtt ctgtctggct gcccttcaat aaaagtaatt gagacagtct gcgccattct
    15601 tctggctcga aagaaagttt cttcaactct tcataaagag ctgtgatccg ttgcgttgtc
    15661 tcttttgtcg gcactccatc caccacacgt acttgataat catctattaa attttctgca
    15721 ttttcgacat aggcttctaa aaaggaagtt cccaatgcat tctgtaaaag ctgaatagct
    15781 tccaggtttt gattaaaccc ttgttccatt ttttctggta acatggtgca cctcatttct
    15841 agatatatta taacaatttt ttcacctgtt taattctatc gaacaattga caaaatttca
    15901 atcgttttca ctgtttttta tttgtgaaac ggcaaacatt tttttattaa aaacagtgtt
    15961 ataacagaaa agagggcgtt ttcacgaaca ttccatcatt ctgtactagt atctgtatca
    16021 ttttttactt cttctgtgaa ccgaaagata aacggtgaga cttccacctc taagaatagg
    16081 gactgttgta cccactgata tgttacagat ccagttgtgt agtaaaaaac tccttccgaa
    16141 ctttcggcaa gcgtagactg ttccgataaa gccatctgct tcattatttt tgctatataa
    16201 taatttttga ttcgtatatt aagatccatt gacaaacggt agctttctaa tgctgccatc
    16261 aataaaaacg tacataaaag aaataaaagc aagacagaaa ataaaataga gcccttataa
    16321 cattttctac tgatcgtttt ttgtccatat ggcatatcca tcttctccat tttcaaagtg
    16381 tacataaaaa gaaattgtgt gttctttttc caataattga agctcagtga tacttgttaa
    16441 cataggttga tgtccgccgg aagttcttct tttacgtatc attgcctgat atctttcgat
    16501 gatatactgt ttatcctcga tcgtgtagtg cagttgctga tttttcacag agacattgtg
    16561 actgctcttg agttcttctt ctaattgtgc taaaaatatt agccactctt tttgatcttt
    16621 acgctgcaaa aattcactta cttggatggt ttgctgaatc aaaagattga atgtgagcaa
    16681 tagactgctc aatatcatca gtccaattag acattcaatc agcgtaaatg ctggaattac
    16741 ttgttttttc aatactgatc gcttctttct ccatattgac aaacactttt tcgattactc
    16801 cattattatc gacaatttgg atatcgtatg ggatcatttc ttcccttcgc tctggtcttt
    16861 tgtgatacat ccgctgcgtc ttgatatctt cataaatcat tcgaataaat agcagttcct
    16921 ccccactttt cttttctgcc tgaagcaaaa acagctggcc aaagctcact aagtagacac
    16981 agacagaagc gatcataagc gctgtaaggg actccaacaa aacaaatcct gttttattca
    17041 attctttttg tatagcgacc actccctatc tggaattgat acgtaatcgt ccgctctttt
    17101 tcttcccaat aaaattgata agcttttagt gaactttcat tcccggttct agcagcaaaa
    17161 gtaatagaag catggcgttt caaggtaatc tcatcaggaa tatctaatac ttcccaattt
    17221 attttatctg gattcggaac atgaaaaatg atttgattct cttcactatt ccaataaaag
    17281 cctgtttgga tctgattcac aatagcgatt ttttgcgtag aatagattcg tttttcaaat
    17341 tggccgaaaa attgatagac tgccaattca ttcttccatg cagaaaatgc gagaactgga
    17401 aaagaaagga tcgttgtaag taataaaatc agtaagagtg attcaaacaa cgtatatcct
    17461 gatgctaaaa ccttaccttc aaggccgctg atagatttct gcatgttctc gtttgatata
    17521 gccttcattc aacaattcct ccacagtagg aactcttcca ttattttgaa gcgcatagag
    17581 ctctttttgt gtgtctacca actggatgat cgcttcacga ctttcttggt ctacatgatt
    17641 cctataacgc gacaaattag ggacgaacaa caacactaat acactgatga tcagtaagac
    17701 gattagcatt tcgatcaatg taaaaccgct ataataagat atttttctct ttttcatggc
    17761 aaaacctcct ctatgcttcc gtaaacaggt aataaaacag cggcataaac taataaaatc
    17821 aaagccgcta cgcccaaaaa aatgattggc tgaataaaat gcatccagtg atgaagacgt
    17881 ttgaaaagct gttttcttgt ccattcgctg taaaaaatca gttctttacc taaacttccc
    17941 tttgcctctc cctgtaaaac aattttactg aattcttctg atagaaaacg ataattctgg
    18001 aactgctcag ccaatgacat acctgcctcc aatccttgaa tcaacttaga ggcaagatgc
    18061 tgaatcagac tttcttgttt cgtttccttt aagcagacta tgatttgcct taattcgaaa
    18121 ccttcatgaa aaagctttcc aatctctagt gacaaataag ccgattgata caaggaatat
    18181 atcggtccaa tcaataattg tctgcttatc cattcactct gatcaacagg agacaacttc
    18241 atcagacgat accttactag taaagccaat agagtaaccg ctaggatgaa tagtagaaca
    18301 taccaatgaa agacttgtat cagctgaata ccccaatgct cttgcatgac cattcctgaa
    18361 ccaataagct gaggcaaaac gaacaaccgc atactaaaca agatacctag caaaaaggtg
    18421 agcaatagca tcggatatgc aagcagcttt ttcaaatcat tgcgaaatcc ttcaacaagc
    18481 cgaaactgtt tggcaatttc caacaaagtt gtcggcagat tcccatgacg ttcagctaac
    18541 tcgatctgta ccagttgctc tgctccatat cctatttgcg aaagtacctc actaaacgct
    18601 tgcccttcct ttagctgatg ttggatattt gccaaaataa caggtggaaa cgaacggatc
    18661 gtgagtaata aataaaggga ttcttgcaga ctaaaaccag ataaaagcag ttctcccatg
    18721 gtttgtgcaa actggttttg ctggcttctt gttaatcctt tagaatagtt ctgactgcca
    18781 aatattttcc tgagcttctt cataactttc ctcccatgat ttttttactg attcttcctc
    18841 cataaattga taactccata aaacagaagg atggtgattg gcatcttcca aaagttcttg
    18901 ataaatgaca ccagctaaac attcagatag ctcttctttt ttctttgtca gttcatggat
    18961 tctgaccaat gtaccttcaa gattgcgtgc atgtacggtt gcaaaaaccc ggtgccctgt
    19021 tagtgctgct cttaccgcag catgtgcagt tgattcatct cttatctcgc caatgattag
    19081 caagtctgga cgatgccgta atgcgagtct caaaagtgta tcataagtct gtgctatttt
    19141 cggattgatc tgtagctgca gaaaaaacgg ttcttctatc tctacaggat cttcgatagt
    19201 aatcacttgc aatttttctt ccaaagccaa ccgatacatt agagaagtct tgcctgatcc
    19261 gactggtccg ctgaaaaggt ataatccacg tctgacggtt ttctgtaaaa tagttgcaaa
    19321 atctcgagaa ctaaagcaac gatggggtgt ttcaaaagta ccgtataata gccgaatcac
    19381 taaactctct cgttgaaggt aatctcctac agaagacaac cgcagacgaa cttcctgttt
    19441 ttctaaagaa taagtaaatg cacctaactg agcttttctt ttttctccga tatccatctg
    19501 tcccaaatac ttaaatcttg agatcagttg ctgtccatct gccgaagaga ctttcttcca
    19561 gaatgttcgt ttttttccat agcgaaaata gatgtaaaat acttctgaag ctggcaatat
    19621 atatacgtct tgggcatgtt tggcaaaact ttcacttatc atttgatccg ataatcgttt
    19681 tatttccatt attctcccct ccacaaaaaa catacgtctt tttgatcttt ttgtttcaaa
    19741 taaaatgaat ttacaaaaaa atattggcaa aaaccgaatt tatgttatac ttataaaaat
    19801 ttatcttggg aggcactcca ttggaaacaa cattaaggaa atatatcgaa gtagcaagta
    19861 aaaaaaagcc tgctgaccta gtgatcaagc atgctaagat cgtcaacgtc tttacgaaag
    19921 aaatcatgga aaaagatgta gcaatctgcg aaggaatgat cgtaggaatc ggtgaatacg
    19981 aaggaaaaag cgtacataat gctaatgggc aatatctagt gccaggattc atcgatggcc
    20041 atgtgcatat cgaaagctca cttttatcac caagcgaatt ttcaaaagta tccttgcttc
    20101 atggagttac tactgtagtg acagatcccc atgaaatagg aaacattgct gggagtacag
    20161 gtcttgagtt tatgatagaa gatgcaagac aaacactcat gaatatcttt tttatgctac
    20221 cttcttgtgt acctgtaacc ccttttgaga ccaatggtgc aaatttaaat gcaaaaaaat
    20281 tagccccttt tttacaaaaa cccgaagttt taggcttagc tgaagtcatg aactatcaag
    20341 cagtagcaaa taatgaaaca gacatcctag aaaagatcca attgatgcac cagcataaga
    20401 aaaaaattga cgggcatgca gctggtatcg gcatggaaga gttaaatgtt tatcctgcag
    20461 caggtattcg gacggatcat gaagctacaa ctgcaaaaga agctaaagaa cgtttggatc
    20521 ttggtatgta tttaatggtt cgagaaggga ccgttgcaaa agatctggca tcgctactac
    20581 cggcgatcac acctgaaaat tcaagaagat gcttctttgt caccgatgat aaactgatca
    20641 atgatttagt aaaagaagga tcaatcgatc atatcgtccg tcaagctatt gcacttggaa
    20701 tcgatccatt acaagccatt cagatggcaa cattgaatgc agcagaatgt tttgatttga
    20761 aaacactagg tgcgattgcc cctgggtatc aagctgattt cttcttaaca gacgatctta
    20821 ccaagctgcc aattcatgaa gttttctata agggaacatt agttgttcaa tctgggcaac
    20881 tccaagacca tttatttgct gatcattcaa attcatatac gtgttgtctg ccaaaactaa
    20941 acgctcaacc attaaatcca cattcgttag agctgcttct ttcttcttca caagcccatg
    21001 tgatcgaaat tatcccgaat agtctagtga ctaaagattt ggtagaaaaa gtggaccgac
    21061 aaggcgaacg attcattcct tcaatcgaaa aagaccaatt aaagattgcg gtgatcgagc
    21121 gtcaccatca aacgggaaac attggattag gcatagtgaa aggatttcag ctgaaacaag
    21181 gagcgatagc gacaacgatt gcccatgact cgcataatct agttgtcgtt gggacaaacg
    21241 acgaggatat gctgtatgct gctaatcttt tgattcaaaa aggtggcggg atgaccgttg
    21301 tcaacaaaca gcaggaaata gcttgtgttt ctcttcctat aggcggaatc atgacacagg
    21361 aacctttttc gatcgtctat gaacagttga atgaattaat aactaaagca catcaactag
    21421 gagcagctaa aacctttgat ccttttttga cactttcttt cttaacatta tctgtcatac
    21481 ccgaattgaa gatcaccgac aaaggattat tttcattttc tcgcttccaa ttgattccac
    21541 cttgtggaaa aaccgatgaa ataaatacat tttaaatcaa ataaatccca gaaaatacat
    21601 tacatctgta atgttcattt tctgggattt atttatggat attaatgttt ttatgaaagc
    21661 agaaagttag tttcacagac ttttcttttt ggtcttatct tgaaattgag cttctactcg
    21721 ataaatacgt tgaccttttg aagaaaattt ttcttcatac tccgtcataa tattgccttc
    21781 aaattgactt tgatgtaaat ctaaccatac ctgtttgagg atcatgccat attgagaaaa
    21841 acttgccaga gaatactcaa acaatccttg attatctgtt ttgaaatgga tttctccatt
    21901 tggacgaaga atctgttcat ctacagcaag aaaagtctta tatgtcaatc gccgtttttc
    21961 atgacgtttt tttggccatg gatcggaaaa attcagatag atttgatcta cttcgctgtc
    22021 agcgaaatac tctgtcaacg ctgatccatc cacatgtaga agtttcaggt taggaagcgg
    22081 ttgttcgatc aacttgtcta gtgcaatcga aacaacactg acctgcattt ctactccaat
    22141 ataattgatt tcaggatggg cttttgccat tccagtgatg aactggcctt tccccatacc
    22201 aatctcaatg tggattggat gatcgttttc aaacaattca ttccattttc ccttccacaa
    22261 tgttggatca ctaattacaa aattcgggtg agccgctaac atttccgctg ctcccggacg
    22321 atttcggaca cgcattattt atctccattc ctttcctcta attaaaaagg tctggccaaa
    22381 tctccgcaga gtttgggcca aacctcagcc actgaaaatt cgttttaatt gtaaaatttc
    22441 ttcgttcatc cgtcgatgat cttcacgttt gcaatatctg gtgatttctt ggagcaaatt
    22501 catggccgca taccaatgaa atttttcaag catttcgtca gtcgggcgta tgccataact
    22561 gatcagccac tgattccagc tagacagtgg cacataattt cctaaaatcg ttgcaatatc
    22621 caccgcaggg tctgcaaaca tgattgaatc ccaatcgact agatataaat aatttttaca
    22681 tactaaccaa ttacgatggt ttacatctcc gtgtactact gataagtttt ccttatctat
    22741 tgacgggatc tgctcacgaa gaaaacgatg gacaatttgc agaaattgat ttttctggag
    22801 ttcttccggt aattcttttt catatttttg cagcatcttc tccggcgtca tgacacgacc
    22861 accgatacgg gtcagcatgg ttttaagaga atttgaatga tgtaaatggt acaaaacatc
    22921 aatcacgtcg tttctttgac caatctcatt aggagtcagc aagcgacctt ccagccactc
    22981 ttgtgctgtc aaagtatcgc cattccccgt tcgttttgtc caaacaagtt ttggtgtaat
    23041 tccttccctt gaaagagcag ccaacatcgg agtcgtgttt cgtttaataa agactttgtc
    23101 ctcatcacgc atgcctatgt atgtttttcc tgtgtcgcct ttgatgggac gtaaccgcca
    23161 ctcttggtct aactgaaact ccatcatgca ttctcccgtc ctcttactct ttgttttgca
    23221 gtacttatta ttctagtcgc ctgaacacta cacgtcaagc acatatacgt tctttttcat
    23281 ttaattgcca ttcaaaaaac cagcttttct caaaaaaaat agaacgaaca gcattcttac
    23341 tgtcaacgct ctatttttca ttctcatttt taccctacac agctttgttt tcagtctgac
    23401 atcttcttca aacgagttgg tacatatagt ttaacaaaga gcatagtaac aatcaaataa
    23461 gccgctagcg gaacccatcg ctgctgccca tctaatacga ctgcagcaat caaaccaaaa
    23521 ataaccgacg cgataattaa tacccatgtg agtagatact gaatcgcctg cttcttctga
    23581 ttcttatgga ttggataaag ctgaacgaga accatatagc ggaactggtt cgctaaaggt
    23641 aacagttgga aaccgatcaa atataaaaat aagaaaccga taatcagact aaaataaaag
    23701 tcgactactc cggcaatcaa taacccacct ataattacta atcggaaata aagaccacta
    23761 aaatcagaac ctcttgccaa tcttcttgca aacaagtaaa gataagtgtt ttttgattct
    23821 cttttaatat gagtcaatat aggatccaaa tagcgtcttc ttttgatctt tgccgtaatt
    23881 tcaggaacat ctgtgaataa attgataaat tgatagatct gatgcaacct atgttcttct
    23941 acatgcacca ttttctccca atccaatcct ttttcttgtt gcttccacaa aaaccagtaa
    24001 tatagacttg cctgtactaa tgcacctgct attcctgccc atattagcca ataaaatgat
    24061 agaaacaaaa agatcagaga agaaaaaaac cagcctaatt tccaataacg aataacttgt
    24121 tgatctagtc gaaacaattc tgctctcttg atttgcagat gactgaattt caaactccat
    24181 aatatgatca gatagacgaa aaacatcgaa aaggattgct ccgtactaac caccactaat
    24241 ggcatggcca atccgcatgc caatatcaaa aagatcaacg gaaaaatgca agaatagcga
    24301 aaagctcgtt ccaaataggt ttgcatttct ttttctttag gaagtaaaaa aacaatatct
    24361 gctgcctctg caaaagtagc catcttaccc acgaataaac agagcatcca aaatacagag
    24421 attatgagcc ctccaattgc aaacggtggc tgcagggttt tcaaccaatt agaataatat
    24481 aatcccacac cgccaatcaa gaaaagacaa actaaaacaa aatgatcgtt caatacataa
    24541 cgcatatatt tcatcatttt cttttgatgt cttgctaaac gtttttgaaa aaaactactc
    24601 atgtgcctgc tcctttgtta aagcaatata gatttcgtcc aaagaagaat ccggcagctg
    24661 aaattctttt ctcaacatct caagagaacc gtctgctcgt atctctccgt tatgtaagac
    24721 cacaaaacga tcacaatatt tttctgcagt agctaaaata tgcgtagaca tcaaaatacc
    24781 ggctccttct tttttcattt ctaccatcaa ttcaagcaat gcattgatag ctagtggatc
    24841 caagcctaaa aatggttcgt cgatgatata taaagaaggc tcgatcaaaa aggcgcataa
    24901 gatcatcact ttctgtttca ttccctttga aaaattagct gggaaccagt caagacgatt
    24961 agataaccga aaagtctcca acaacttttc tgctcttttc attgctatat ccataggtat
    25021 atcgtaggcc atcgcagtga cttcgatatg ttctctcaat gtcaattctt catataaaga
    25081 aggagtctct ggaatatacc caatttgttt gcgatattct tccgtatttt tctttaagga
    25141 tagatgattg atttctatcg ttccttttga agcttccagc agaccgataa tgtgcttgat
    25201 ggtcgtactt ttccctgctc cgttcagtcc aatcaaccct actatctctc caggctgtac
    25261 atcgaaactg atatctttta acacagggag atgtccgtac ccccctgtga catgatcgat
    25321 aattaaactc attctttttc ctccgccatt tatttttatt tattatacca cgaagaaaat
    25381 gacggtattt tgtttttttc cttacaatag ctttaactta tggtaaagtt aagctgaagt
    25441 atttattctt tatagaaagg atgttttata tggaaaactg tattttttgc aaaatcatca
    25501 atcaagaaat cccaagctat aaaatatatg aagacgacaa ggtctatgct tttttagaca
    25561 tctctcaagc aacgaaagga cacacattag ttgtgcctaa acgtcatgta gcagatattt
    25621 ttgaatatga tcccgaactt gcaggagagg ttttttcgcg tgtgcctaag attgctcaag
    25681 cattggaaaa agcttttcca gaaatgaaag gtcttaatat cttgaataac aaccgtgaac
    25741 tagcctatca atctgttttt cattcacata tccatttagt ccctcgctac agcaaagagg
    25801 atgatttttc tattcacttt gtcaatcatc aagatagtta tggatcggaa gaactaaaag
    25861 ctattcaaga aacaatcgta aagcaggtgt cctgcgatga ttaaaaactt tttcaaagga
    25921 ctgttatttg gagctgctgc tggcactgtc ggaggtctgt tattggcgcc caggagcggg
    25981 aatgaaacaa gaaagaaact gatcgaagaa ttggaagaag caacaactct gacaaatgat
    26041 ttaaataaca gcctgaatca ttttaaaagc gcattgatcg aaacaaaaga aacagctgaa
    26101 tctgtgattc ctgctttcca agaagcaatc aaaaaagata tcgaagattt taaattccaa
    26161 gccgaaccac gtatcacaca aatcaatgaa caagtagaaa aactaaaagc tgatctgcca
    26221 gagtatcctg aacaaatata ataatgatta ttttctcaac agatttaact atataagtga
    26281 gaaaagtttg cgtatttcct aggaatgtga tgaaattttg aattttgttt gaaactacca
    26341 cgacaacgtt tttaaaggaa atgggatatg gtatagttaa tatataaaaa taataactaa
    26401 ttacaggagt gcaaatttta atgaaaaaaa aatcaattat tttggcagct actagtgcgt
    26461 tggctgtttt aactctagca gcttgttcag gcgatactaa taaagatatc gcaacgatga
    26521 aaggcggaac gatcactgta tctgatttct atgatgaagc aaaacttgaa tcatcgaacc
    26581 aatccttagt acaacggatg atcatttata aagtattcaa taacaaatat ggcgataaag
    26641 taaccgataa gcaagtggat gcagaatacg acaaacaagc gaaatcatta ggtgatactt
    26701 ttgaatcaca attagaagct gctggatata cgaaagatac ttataaagaa tatattcgta
    26761 acaatttagc ttttgaagct ggcttgaaag ctcacgtaga tattacagat gacgatttga
    26821 aaactgcttg gaaatctttc catccagaag tagaagcaca aatcatcaaa ttatcaagtg
    26881 aagatgaagc aaaagatgtg aaaaaatcag cggatgatgg agatgatttc tctaaattag
    26941 ccaaagacaa atcaactgat acagagacaa aagaagatgg cggtaaagtt aaattcgact
    27001 caacgacaac aactattcct gcagaagtaa aagaagcagc tttcaaattg aaagacggtg
    27061 agatctccga tgttattact acaacaaatc ctacttctta tgcgacagaa tactacgttg
    27121 tcaaaatggt gaaaaaccaa aataaaggca atgacatgga taaatacaaa gatcaactaa
    27181 aagatatcgc tactgaaact aaactaagtg acaatgcatt cacaactaaa gtaatcggtg
    27241 aagaattaaa agatgcaaat gtaaaaatca aagatgatgc ctttgaaaat gtgttgagtg
    27301 cattcacaac aacttcatca agtacgaaag actcaagtga aactacagct tcaacaaaga
    27361 gttctgacac aaaatcaact gattcaacaa aagaatcttc aactgaagaa acaactgatt
    27421 catcaaaata aaagaaaaca aaaaaagcga agacttatgt ctcgcttttt ttgttttgaa
    27481 tgaaaaaaat aaatttttgc tataaatctt ttggtacata gaaacttcgg ttgtccatac
    27541 cgaaaatccg atcggtgtat tttcctggtt ctgtttgacg aatcgaagtc aacatcatct
    27601 gcatcgaagc atcgatattg tcgatttgat gtaatatttc tgcttccatt attcttggac
    27661 gtacaggtga gccatattct aataagccat ggtgtgctaa aaccatatga cgtaatacga
    27721 gaacatcttc gtcagcttcg ctgattttca acgcaagaca ggctttagtt atttcttcgt
    27781 caatcaatac cagatgaccg atcaaatttc ctgcaagcgt atattctgtc gacataggac
    27841 cggaaagttc aatcaccttt cctaaatcat gcaaaatgat accagcatac aacaatgctc
    27901 gattcagctc aggatattca tcggcgattg atttccctaa gtgaagcatt gtcaaagtat
    27961 gataagctaa tccactggca aatgcatgat ggtttctttt tgctgccgga aactcaaaaa
    28021 attgtttttg atatttatta agtagatagc gaacaatacg attccaatgc gcattggtga
    28081 tctcaaacaa caattgattg atttcttctt ccatttcttc tttcttcaaa ggggctcttt
    28141 ccatgtataa actaggctct cctggctcat ctacccttgt taagcgcata tgaagaatct
    28201 ttacttgagg gtttccttga taaacctctc ttttaccgtt tagaaaaacg actgtgccgg
    28261 ctgtaaattt attgatttcc tcctccgaag catcccagta tttaccatcg atagtacctg
    28321 aagtatcttg gaaagtaaaa gcaataaatt tttttccgtt tttagcgatt cttacatctg
    28381 cacttttgat caggacaaat tgttcaaact gctcatcgat tactaaatca cgaatttttt
    28441 tcattatttg ctccttctaa ttgttttacg atttggttca tctcatggta atagcttacc
    28501 atttctttat cagaagaaaa acaaattatt tgatactttt ctccaaattt agctaaaagt
    28561 tcagctaatt gatactttcg ctgatgatca taatgaagcc agccatcatc aatgatgatc
    28621 gggcatatcg aacggttagc ttctaatgag aggtaagcaa atctcatcgc cataatcagc
    28681 tgatctttcg tacctgtaga taactcatag atgggaaatt ctgtatatcc gtctgttaaa
    28741 gtcagtgtat catttttcag taatatcttc tgataatttc cttttgttaa aatacctaaa
    28801 taatcacctg ctgctcttaa caactggggt aattgtcttt cactcatctc tgttgaaaga
    28861 tcacctaaga atgtcccggc gatttcaagt gctccccatt cctccagtaa ttcctgtaat
    28921 tttgcttttt gccggctagc ttgttgataa agttcgtcca atgtgccgtc tctttgcaac
    28981 tcttctactt gaagttggag tcgttgccga ttttcacttg cttctttttc ttcttgctgg
    29041 agttgggatt gcttttcttt cagttggcgc agtcttacag ataatttctc ttttgtcagc
    29101 tcttcaggaa atagtggagt aagcatttcc gataattcat tgtatctttt catctgctgt
    29161 tcggcttgtt tggctttttg taaaaagaca ggtacttccg aaggataaga taagcctgct
    29221 tgagtcaatt gttcgctgta ttcttgtatc catacttttt gtttcttttt caattgattg
    29281 atttcttgct tcaataaagt attttgctga tagcttctgg caaatttcat ctgttccatc
    29341 tcttgtataa aacgggaaag caataccatt ttttctgaaa ttgtcttatc ttgtatcggt
    29401 aaccattctt taacaaactc aaatttttgg tcgatttgtc gtagtttttc ttgtatttcc
    29461 tgttctcttt tatgtttccg ctcaaatacg ccatataatt tctgataatc tatgacttcc
    29521 tgtccatatt ctttcatgag tttgagcgta tccatctttc ctaagtgccg atgatccgca
    29581 aaacgatata cttcttgctg aagaatctgt ttttcttctc cagttctctt ttgtttttct
    29641 tctgcttcag ctaattgagc taaggctaga tcaagttgtc ctagtttttc ttgccacatt
    29701 ccttttatct cagagaaatc atttttagtt ttaatttttg gttgcctttt gtagaagaaa
    29761 tagccaatca tgcctgcacc aattagtaaa aggatcactt tcaagggcat tggtacaaat
    29821 atacctgcaa tgattattgc tccgccagcc atcagcatat acggtttaga tgaagagact
    29881 ggtgctatct ctgactgatt ttgtaataat gctggatagg tctcttccaa gtcattcact
    29941 tctgcttcaa tcatctcttg ctgttcttta gctatttgga gacgcacaga aatctggctt
    30001 tctagatcag caatccttct ttcttcagcc agaagatgct tccattcttc ttcatataaa
    30061 aattcttccg gtaacggatt tttctgccaa tgccaccttt gttccaaaat cttcaattct
    30121 gaatttgttt ctctagtctc aatttgtaat ctttgccatt cttcgtttaa ttgttccgct
    30181 gtaaattgaa gttgctggag atcttttagt tcctgctcct gctctaaata aaaataatat
    30241 ctggctgact gctgatccat cccgctatgc ttttccagtg ttgcttctaa ttcttccaaa
    30301 cgctcatttg tttgctgata ccgtttggag aaagactcca gatctaaaag aagttgctca
    30361 tcaggaagac ctgtagtgct gttttttttg agagattgcc attcttcata taaagaaaaa
    30421 ttaagttctt gctgtctggc taatgaaaat tgtttgttcg cctcttttaa ttgtgccaat
    30481 aactgctgct gttgcgactc gtattcctgt tcttgttgga tcaacttgcc aaaataagct
    30541 tcctgttctt gtttttgatg aattttttct tgcagctctt tccattcatt taatttttga
    30601 ttgattagta atttttggcc ttttttcttg aataattgtt gtgctttctg ataatattct
    30661 tgccgctttt ggaatacttg agaactgccc gtgatgccta aagaaatcag cgcatcatgt
    30721 agctcttttt ctctcaaatg atctagttcc atcaactgct cttgctggaa agtaaatacc
    30781 tgctggaata actctttcgt caaaggtgcg atcagttttt ctagtaactc atcacttccc
    30841 acttgatccc ccagccacac cttcgatttt ccgcgatttt gctgcttgta gcgctcgaca
    30901 gcaaattcac catatacagg atgtttaagc catattttgc cgccataggc tgagccgtct
    30961 tttggtgtat aatccttttt tttacggcct tttgaaggga aaccaaataa tattgcttga
    31021 ataaattgat aaatcgttga ttttcctgct tcgtttcccc catagatcag ctgattgcct
    31081 gaataaaagt caaaactttt ttgacgccat tttccaaaag cagcaatctc gatccttaaa
    31141 atcttcattc ctgatcctcc tcaaataaaa aatcttgatt aagcagctgc ttggcttttt
    31201 caagtgtttc ttcttgatat tccggcagtt cgctcaatat tctagccgct tcttgatgac
    31261 tatatacttc atttaaaatt tgttggaaat cttgaggtgt ctgatagtat tgaaataact
    31321 gttccatcaa tttagcagaa gcagcaagtg gggtcttcgt ataaaaagta ttctctatga
    31381 gagaaatacg ccaaataaag aaatcattta tcctatctga gaattcctct aacaaatagt
    31441 ctaatagctc tcctgattgt atccgttcca aaacatctgt attgagatgg tcataatctt
    31501 ttaattttat ctcaattaaa ctaaatccat cctctacttt tgaaagctgc tgacgaatgt
    31561 gaacaatgac ctcttttgtc gttcttgctt tcctcaatga tatttccttt gtcttccaat
    31621 agacctctgc tactttaatc ggatgtattt ggcaacttcc tttacttaat tcaactagta
    31681 gaatagatgt agaagcttgt tcctttttag tatgaccttg aggcgcacct ggataaacaa
    31741 ttgtttgctt ttcatttaag accgttggca catggatatg ccctaaagcc caataatcgt
    31801 atcccttttc ttgcatcttt gagggttgga aaggagcata gtttcctttt tgagagacgc
    31861 caggttcccc gtggtacaac ccaatatggt aatctgtgag ttctctagaa ggaaattcca
    31921 tcactttgtc tttttgaatc cacggttgac gataactaaa tccactgaga gagacagttt
    31981 ctccactttt tatcgttatt ttttttgtct ctaccgtttc ggatgtaaat aaatggacat
    32041 ttttaggaaa ggagaaccaa taccgctctt tttgatagaa atcatgattt ccgaatatta
    32101 taaatacagg tatatggttc ttttctaacc gtttcatctg ttctgaaaaa tgtttctgta
    32161 tttttaatga aggacgattt tgatgaaaat tatctcctgc aagtaaaaca aaatcaacgt
    32221 tatttattat tgcctgttca atgatatttg ataaaacggt cagattcgtc ttcaatagtt
    32281 tttcctgtac agttttatca agagttgtca gtccttcaaa tgaccgatcc atatgtaaat
    32341 ctgccgcatg gataaatttc accattaaaa atttcctcct ttttttcttt tatcatacca
    32401 ttttcaatgt tttttttcat caataaccga aaatttgttc gtattatgca aagaaattac
    32461 gataaatggc cgacttcaaa aaataagttt attcgaaaaa aagattgtga catgattttt
    32521 gtcacaatcc taaatatcta tgttatattc gcttcgtttg acactgaaga agtactattt
    32581 gtactggaat ttatgctcca tttttccgta tttagaattc tatgttctat actaccttaa
    32641 ttagtttata tggtaagaat tctttcaaat gcttgagata ctccgtcttt atcatttgaa
    32701 acagtgacat aatcagcttt tttcatgatt tcttgatgac tatttcccat tgcaaaacta
    32761 atatctgcac tttcaaacat agataagtca tttaaactat ctccgatcgc gattctcaaa
    32821 ggtttcttta aattgtagat tgattccaaa cgatcaaaca ccacaccttt agatacattt
    32881 tgaggtacga tttcaatatt tacaggtgcc gattcagtaa cagacacatt tagcatcttt
    32941 ttcaaatggt taaatatatt agctttcaat tgacgattag gaataaaaat aaaaaatttg
    33001 taaatgctga tagcatcctt ttctataatt tcctcaatat tattataagg gattgaacaa
    33061 atagattttt catactcaag ctcatagttt agctctttga tagaaaattc actccctacc
    33121 tcgtgaaaaa tttttttcat tttattttta taattctgag attcataact tccgagattt
    33181 gtatatattt tggttggaaa tttcgatagg acagaatcaa ttttttttac atcattttta
    33241 tcgaaggaag actcaaatat ctttttttct ccatcaaatc caacagaacc atttaaccct
    33301 actgctggta tccttaaacc agattttttg agaatattat taatgtcttc tacttctctt
    33361 ccagaacaaa tgaatggaaa acagtcttcc ggtaaatgtt tcattgttct tgcatttttt
    33421 tgactaatgc tctggtcaga attcaaaaga gtaccgtcta aatctatacc aattagtttc
    33481 atttcatcca tgtctcctat acgttgatta taaattcact gcttattgta agtctaaatt
    33541 tctcacaaaa catatactaa ttatacctaa tttaagtaag tacaaacatc attttaagaa
    33601 taggaaagga catttatata ctattctctg tatttttgat ttataaataa atatgaattg
    33661 aatccaaatc agctatatta ctattttata ataaccaact ataaaaaacg aagttaggtg
    33721 aaaaactaac ttcgatattc ataaaagact ctattagttc aacttagcaa aagatttaaa
    33781 caaaaaaaga tgaactattt ctagttcatc tttttgcctt
//
LOCUS       DAZKCC010000022        32505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000022 DAZKCC010000000
VERSION     DAZKCC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.022"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG8VH_001147"
     CDS             complement(<1..563)
                     /locus_tag="ACG8VH_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFP9109719.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG8VH_001148"
     CDS             complement(750..1400)
                     /locus_tag="ACG8VH_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109720.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8VH_001149"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8VH_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP9109721.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8VH_001150"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8VH_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9109722.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8VH_001151"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8VH_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9109723.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG8VH_001152"
     CDS             complement(4926..5657)
                     /locus_tag="ACG8VH_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP9109724.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG8VH_001153"
     CDS             complement(5661..6926)
                     /locus_tag="ACG8VH_001153"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP9109725.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8VH_001154"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8VH_001154"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9109726.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8VH_001155"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8VH_001155"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9109727.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG8VH_001156"
     CDS             12268..13770
                     /locus_tag="ACG8VH_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9109728.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG8VH_001157"
     CDS             13772..14509
                     /locus_tag="ACG8VH_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9109729.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG8VH_001158"
     CDS             complement(14680..15873)
                     /locus_tag="ACG8VH_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9109730.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG8VH_001159"
     CDS             complement(15952..16158)
                     /locus_tag="ACG8VH_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9109731.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG8VH_001160"
     CDS             complement(16151..17293)
                     /locus_tag="ACG8VH_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9109732.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG8VH_001161"
     CDS             17805..18440
                     /locus_tag="ACG8VH_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9109733.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG8VH_001162"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG8VH_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG8VH_001163"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG8VH_001163"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8VH_001164"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG8VH_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9109734.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="ACG8VH_001165"
     CDS             21086..21253
                     /locus_tag="ACG8VH_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9109735.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="ACG8VH_001166"
     CDS             complement(21313..21666)
                     /locus_tag="ACG8VH_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109736.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="ACG8VH_001167"
     CDS             22170..22589
                     /locus_tag="ACG8VH_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9109737.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="ACG8VH_001168"
     CDS             22615..22821
                     /locus_tag="ACG8VH_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109738.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="ACG8VH_001169"
     CDS             complement(23049..23222)
                     /locus_tag="ACG8VH_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109739.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23234..23866
                     /locus_tag="ACG8VH_001170"
     CDS             23234..23866
                     /locus_tag="ACG8VH_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HFP9109740.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEEVHLFKKTLACFSKNN
                     GSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(23948..25114)
                     /gene="dnaJ"
                     /locus_tag="ACG8VH_001171"
     CDS             complement(23948..25114)
                     /gene="dnaJ"
                     /locus_tag="ACG8VH_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9109741.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25265..27094)
                     /gene="dnaK"
                     /locus_tag="ACG8VH_001172"
     CDS             complement(25265..27094)
                     /gene="dnaK"
                     /locus_tag="ACG8VH_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9109742.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27145..27708)
                     /gene="grpE"
                     /locus_tag="ACG8VH_001173"
     CDS             complement(27145..27708)
                     /gene="grpE"
                     /locus_tag="ACG8VH_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9109743.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27802..28845)
                     /gene="hrcA"
                     /locus_tag="ACG8VH_001174"
     CDS             complement(27802..28845)
                     /gene="hrcA"
                     /locus_tag="ACG8VH_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP9109744.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29079..30244)
                     /gene="hemW"
                     /locus_tag="ACG8VH_001175"
                     /pseudo
     CDS             complement(29079..30244)
                     /gene="hemW"
                     /locus_tag="ACG8VH_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(30260..30487)
                     /locus_tag="ACG8VH_001176"
     CDS             complement(30260..30487)
                     /locus_tag="ACG8VH_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9109745.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30971..31578
                     /locus_tag="ACG8VH_001177"
                     /pseudo
     CDS             30971..31578
                     /locus_tag="ACG8VH_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            31751..32464
                     /locus_tag="ACG8VH_001178"
     CDS             31751..32464
                     /locus_tag="ACG8VH_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109746.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9523 a         6618 c         5737 g        10627 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagaagt acatttgttc aagaagactt tggcatgctt
    23701 cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa ttatttattc ataaaaacac
    23761 cttgcaatat cgattgaaca agttccattc tctcactggt tataccccta gaaactatga
    23821 cgattatcat attctaaagc tggctttttt gttggtacaa acatgacaac tacaaaaaaa
    23881 ccaggcgaac atatgattgt tcgtctggtt tttaattatt aatgacttag cgtttttact
    23941 agaaaaacta cttccgcttt ttcccgccaa aagcatcttt aaacttatcg aagaaaccgt
    24001 cttcttgctg ttcttcaact ttgatgccgc cagcttcagc gaatgcacgt aatgcttctc
    24061 tttgttgttc atttagattt ttcggtgtga tcagttttac tttgacatgt tggtcgcctg
    24121 ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa acggaagttc gtacctgtct
    24181 gtgttccagc agggatcttc aatttcacat ctccatggac agttggtact tggacttcat
    24241 cccccaaagc tgcttgtaca aaacttaaag gcaattcata gtaaatttca gaaccttcac
    24301 gatcaaagat atcactttct tctacacgga agaccacata caaatcacca aatggtccgc
    24361 cgttttctcc agcttctcct tgaccagcta aacgcatttg ttgtccttct tctacaccag
    24421 ctggtacatt cactttaact gaatgtgctt tcttctcatg tcctgagcca tgacaggttg
    24481 gacaaggttc tttgatttct ttccctgtcc catgacaaac atcacaagtt tgacggctca
    24541 tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt acctgcacca tgacatttat
    24601 gacaagtagt tggttgtgtg cctggttttg caccgttccc accacaagta tgacagattt
    24661 cttcccgatt atacttgatt tctttttcta caccaaaaat cgcttcttca aaagacaagt
    24721 cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt tggatcgact gaccgaccgc
    24781 ctcctccaaa gaaagagtcg aagatatctt caaaaccacc gaagccgcca ccagagaagc
    24841 cgccgaatcc accaaatccg ccaccagctc caccagcacc gtaatttgga tctgtgcctg
    24901 catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc acttaaaatc tcatatgctt
    24961 ctgatacttc tttgaatttt tcttccgcat caggttcttt attgatgtca ggatggtatt
    25021 ttttcgaaag cttacgataa gctttcttta tttcatcttc tgatgctcct tttgagagcc
    25081 ctaagacttc ataataatca cgttttgttg ccataggctt tcctcctatt ggttctcatc
    25141 attttcacgt cgcttgttat catatcatat tttgtttgaa acaaaaaccg atacttcaaa
    25201 ctatttttca aaagaaaaag ccaaagtagc aactttggcc ttctctcgaa attcagtgaa
    25261 ctgattattt atcgtcgcca tcgacttctt caaaatcagc atcaacgaca tcatctgctc
    25321 cgccttgagc tgcttctggg ttttcttgag cttgttgttg tgccgcttgt tcgtatagtt
    25381 taacagtcaa gttttgaacg atttcgttca atgcatcgcg tttagttttc atttcttcga
    25441 tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc tgtttctgct tttttcactt
    25501 cttcttcgtc cactttgcct tctaattctt tcaatgtttt gtccacagag aatagcaatg
    25561 catctacatc attacgtagg tcaacttcct ctttacgagc tttatctgct tctgcatttg
    25621 cttctgcatc ttttaccata cgttcgattt cttcgtctgt tagacctgaa gaagatttga
    25681 tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc agatacattt acaatcccgt
    25741 ttttatcaat atcgaatgtt acttcgattt gtggaatacc acgaggagct gctggaatat
    25801 ctgtcaattg gaatcttcct agagttttgt tgtcagcagc cattgggcgt tcaccttgta
    25861 atacatggat gtctactgct ggttgattat ctgccgcagt tgagaatact tgtgatttac
    25921 ttgttggaat cgttgtattg cggtcaatca atttagtgaa cactccaccc attgtttcga
    25981 tacctaatga caatggtgta acgtcaagta aaacaacatc tttcacatca ccagtgatca
    26041 cgccaccttg gatagcagca cccatagcaa ctacttcatc agggttaact gatttgtttg
    26101 gttcttttcc tgtttcttta cgtactgctt ctacaactgc agggatacga gtagatccac
    26161 cgactaaaat aacttcgtcg atttctgatt gtgataaacc tgcatctttt agcgcttgac
    26221 gtactggtac ttttgtacgt tctactaagt cagctgttaa ttcatcaaat tttgcacgtg
    26281 tcaatgtcat ttccaaatgc aatggtccag attctcctgc tgtgataaat ggcaagctga
    26341 tttgtgtgct tgatacgcca gataaatctt ttttcgcttt ttcagcagcg tctttcaagc
    26401 gttgtaaagc cattttgtct tgagacaagt caatgctgtt ttcttttttg aattctgcta
    26461 ccatgtagtc gatgattttg ttatcaaagt cgtccccacc tagatggttg tcgccagctg
    26521 ttgacaatac atcaaagaca ccgtctccta gttcaaggat agatacgtca aaagtaccgc
    26581 caccaaggtc gaatactaag attttttcgt cgcgatctgt tttatctaaa ccataggcaa
    26641 gagcagctgc agttggttca ttgacgatac gttctacttc tagaccagcg atcttaccag
    26701 catcttttgt tgcttgacgt tgtgcatcat taaagtaagc tggcacagtg ataactgctt
    26761 tttcgacttt ttcacctaaa tagtcttctg cgaatccttt gatatattga aggatcattg
    26821 ctgagatttc ttgtggtgta tatgatttac cttctacatc tactttgtaa cctgcttcac
    26881 cgatatggcg tttgattgat gaaatcgtat ttgggtttgt cacagcttga cgttttgcaa
    26941 cttcccctac ttggatttct ccatttttga atgatacaac agaaggtgtt gtacggtttc
    27001 cttctgggtt agcgatgatt tttgcttcgt ttccttccaa tactgctact gcagagttag
    27061 tcgtacctaa gtcaataccg ataattttac tcataattaa cgtctccttc tttaattgaa
    27121 agtgtaagtt ttatatccgc acaattattg tgcaacaatg accatggttg gtcttaacac
    27181 gcgatcatgt aatttgtagc cttcttgtag tacttctaca atcgtatcgg ctggtgtatc
    27241 ttcagtcgct ggaacagttt gtactgcttg atgaagatta ggatcaaagg cttctccttt
    27301 agcagggatt ttttcaattc cttcttcttc cagtgcgtta cgcaaacttt ccaagaccat
    27361 ttccacgcct tttttcaaac ctgccccttg atcatctgac acttctgtgg ctaatgcacg
    27421 ttccaggttg tcaattgatg gaagtaattt tttggctaaa tcttgtgaac ggtatttttg
    27481 taattgctca cgctcatttt ttcctcgatt tgccatattt gcgatttcag cacgagcccg
    27541 caagtattta tcttccattt cttctaactc agcttttaat ttcgctgttt cgaattcttc
    27601 cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg acatcgattt ccggttctgg
    27661 ctgagcatct gtcatttctt tatctagttc ttcttgattt tctttcacga tacagtcttc
    27721 ctttcattat tcagtcagct gtttgattgc tcttactaag aaagagagaa cgatcagctg
    27781 caaatagctt cgttattcac attacgagtt tgacaagtca agcgaacggt agtaatcagc
    27841 aagtttagct gctagttctc ttcgataaac atcgactaga ccgaacatcc gagaataagg
    27901 catactagta ggccctaaca aggctattgt acctcttcca tgaccatcta tctcgtaact
    27961 tgcttgaatc atactcatat tctgcaataa atcattgcct aattcagcac caatcttgat
    28021 atgaatattg ctatccatcg gagtgatcat ttgtgtaagt ccgtctgagt ctttcataaa
    28081 agaaaacatc gatttgaact gatcaagatc ttgacggggc tcataatcca agagattcat
    28141 ttgtccactg acataaacct tatcttcaaa aacatgacca agaacggtgt caaataactg
    28201 agacatccct tctgtcgttt ggaaatattt atgaagaatc atcggaatct ccgtacgaag
    28261 tttatggtac accgtcatca gaggatctcc gactagacgg tcgtttacaa tctgtaccat
    28321 tttttctagt tcctgacttc ccatatattc gggaagagtg aacacttggc tttctacatt
    28381 ccccatatct gttacgacga tagcaataat ctgtcgatta ttcaagggta cgatacgaaa
    28441 gccagtcagt cggcggtctt ttatttcagg tcccaatgaa aaagtcgtat agcttgtcag
    28501 ctcagataat atctcagcag attgcttaat aatctcattg atttcatgaa attctctgcc
    28561 tagagactga cgaatcagca gcaaatcatc ttctgctacg cgagcaggtt ttaacaaatg
    28621 atccacgtaa taacgatagc ctaaagcaga tggaatccgc ccagaagaag agtgagtctt
    28681 caacagcaat ccttcatctt ctaatgcttt catatcattg cgaatcgttg ctgagctggc
    28741 cttgactccc tcagacatca atgttttgga accaacgggc acgccactac tcgtatagtg
    28801 ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt aacatcttcc tcacctctca
    28861 ttagcactca tatattctaa gtgctaatcc acttataaat atagcaagaa gcccttctcc
    28921 tgtcaagaga aagactccat tttttagcac tctaaacaca agagtgctaa ttctaaataa
    28981 ttgatgtaaa tccagttata tcaacgtttc aatagtcctt tattgtattt ttgaactttt
    29041 tttatttaaa ttcagataaa aaacaaatat tctttaaact atttttcaaa caaaaagcgc
    29101 tcaaacacat tgttccccaa aaaggtccct cttggtgtca aataaacacg gtcttcttca
    29161 ttaacaagta acccttcttg ctctaattcc cttagcacat caccgtacac cgattcaatt
    29221 gttttttgat agcgatcata gaaatgtttt ttagtaatcc ctgttttttt cttagtccta
    29281 agaacatttc ttcttctatt tgattttttc gtgtcaattt ttcttcttcc aataccgggc
    29341 gttcatttgc tcgtaatggg cgtaaatagt gttggattgg tcctttgttg cggtaacgaa
    29401 tatctccaag ataaccgcta gcaccagctc caaacccaaa atagtggtca ttgttccagt
    29461 acatgaggtt atgttgagat tcatgtccgg agagcccaaa gttacttatt tcatattggt
    29521 gacgtccatg cttttccatt gcttcaatcg tctcttcaaa cattctggtt tccgtttcct
    29581 gatcaggcag ttcgagtctt ccttgtcgta cccagttcat gaacattgtt ttgttttcga
    29641 ggatcaatga gtacaaagaa tagtgaggca gatccaaagc caaagctttt tccaaagtat
    29701 ccagatatcc ttctagtgtt tgtcccggta atgcataaat caaatcgatg cttacattgg
    29761 taaaattttc tgcttctaaa aattgcatag tttgatacac atcttctgcg gtgtgtttac
    29821 gtccaatttt tttcaataag cgattatcaa atgtctgcac tcccatggat aaacgattga
    29881 cgccatagtt tttcattact tgaagttttt cacgggtcaa atcacctgga ttcgcttcta
    29941 cagtaaactc attaccattt tgaaaaggca ggatctctct agcacctgat aacagtcgat
    30001 caagttgttt agcagataaa gaagttggtg tcccgccccc tacgtaaagt gtttctaaat
    30061 tcgctgtcgg atgactttct agcattattt tcatttcatt cagcagcatt tccacatact
    30121 catctactgg ctgcccttct aaaaaaactt tattaaaatc acagtaatag cagatatgct
    30181 cgcaaaatgg gatatgaatg tacgcagaaa ccttgttttc tgcgggattt attgtttcta
    30241 tcattgaaat tctttctcct tacgtcgaaa agtcgtgcct gttttcgacc ttcttaaaat
    30301 cgcgttcaag caccaactca ttgaaataag ccgcatattt agaatcaagg aaacctagct
    30361 ctaaaaatgc ttgcttattg tcatcggagc tacacggtgc ttttacagcc tctcgataaa
    30421 cggtaatcgc aaagctgacc ttcggcatta agtcaaaatc tgcaaaaata tgaaaagcta
    30481 ttttcacaga ttcctcctta agtgctcaga ccacaacgct ttgcttacaa cctctcatat
    30541 acgcatacta aacgtatttg gcgaacaaat ccactaaaaa atccaagaat cgacttcttg
    30601 gatgattttt tctttcatta aactggttac ctagttatat atctctaggg ttgttttcat
    30661 gtctgagtgc ccgatatgat ttatttttcg tttctataat tactttttgc cttccagttt
    30721 cttttaatat tgtactcaag caagtgggaa ttggttcttt tgtcatccat gtatgcaaaa
    30781 tgttataatg ctggtaaaca aaaatgatta cctgtcattt ttatttagtt cgtatttaat
    30841 taagttgact atctgtatac tctgtctaaa acttaagaat tgttgttttc gctgtcgcta
    30901 cccaatttta atgaatgtct gtaggttaaa actgttgtcc ttgttataga tagtaaagga
    30961 gagcttgaaa atgaaaactg cgattacgtt tctattagag gaatatgccg attgggaagg
    31021 tgcttatatt actagcacac taaaccaaaa tgagaaatgg caaaacaaaa ctgcttctat
    31081 ccaatcagag gtcacttcca ttggaggatt aaaaacaaaa atcgattact taatcgaaca
    31141 aatacctgaa aaagttgacc tttttattat gatcggtggc aattcatggg acataaaaaa
    31201 tcatcattta tatcaaataa tcgataaatt gttaaaaaca gacgtaccag tagctgctat
    31261 ttgtggggca gttgattatt tagctcagag cggattattg caaggttaca aacacaccgg
    31321 aaatgcgcaa tttccttggt tagaatcgga aaattatcag aataaagaag attttataga
    31381 acaacaagct gtgaaagatc gtaatttgat tactgcgaat ggaacagctc ctatagaatt
    31441 cacagacttg attcttcaag cagtccattt tgaaacagaa gaaataagag aaaaaacgat
    31501 tcgtctatac aaacttggat tctatgatta ctataaaaag tacggagatc ctttctccta
    31561 atcattctct tcaattaaat actccttatc aatagtgtga caagtaattt tactgatgat
    31621 tatttgtcac actttttctg acatttatca aagaagatac actctattta tattcataga
    31681 caaggtttta cggattcaaa aattcaggat tatgctatac tggtattaaa aaaataatga
    31741 ggtattttat atgaaaaagt taggttttat tttattgttg gtctctttat tccttgtagg
    31801 atgtacgtct cctagtaaag aaaatagtga tccatctgcg accacagaca aaagttcaat
    31861 cagacaaacc agttcagctg aaaaatcaag tactcagaaa tcagcttcta ctgaaacgaa
    31921 tacatcttca acaagcagta aagtctcaga gcccaatact tcttctacat ctagtagtac
    31981 agttgagaca aatatttctt cttcaacaat agacacaacc tcctcacagt cttctcagtc
    32041 aatagatccg ttagcaggat attctgatct acaaattgag tacgcacgtg tttggctagc
    32101 tattagaggt aatactttta gagatacatc gggtgatttc gaattacatg ttattcatta
    32161 tcctgcagga acacctatcg ccccttatga tgcagggagt gcagtctatc cgaaagatgt
    32221 ggttatgctc actgggaaat acagttacca gggagtttta gtgtattcaa gtaaccatga
    32281 cgggacaatc actagttatc cagttccttc acattggcaa atgcctgcag atcaaacatc
    32341 ttatccagcg ttcattagag aaacaactca agaaatcatt gataatgcat ctgtagtagc
    32401 tattcctaca ggaaatccca atgacgtgaa acagttaatc gatgtgacag ttatagataa
    32461 ttagactttc taatatgtta tacaaaaaga agctgacagc atttt
//
LOCUS       DAZKCC010000023        32439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000023 DAZKCC010000000
VERSION     DAZKCC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.023"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..2774
                     /gene="clpB"
                     /locus_tag="ACG8VH_001179"
     CDS             165..2774
                     /gene="clpB"
                     /locus_tag="ACG8VH_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9109747.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2925..3611)
                     /locus_tag="ACG8VH_001180"
     CDS             complement(2925..3611)
                     /locus_tag="ACG8VH_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109748.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3589..4083)
                     /locus_tag="ACG8VH_001181"
     CDS             complement(3589..4083)
                     /locus_tag="ACG8VH_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9109749.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4103..5323)
                     /locus_tag="ACG8VH_001182"
     CDS             complement(4103..5323)
                     /locus_tag="ACG8VH_001182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109750.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5470..7305
                     /gene="lepA"
                     /locus_tag="ACG8VH_001183"
     CDS             5470..7305
                     /gene="lepA"
                     /locus_tag="ACG8VH_001183"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9109751.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7501..8412
                     /locus_tag="ACG8VH_001184"
     CDS             7501..8412
                     /locus_tag="ACG8VH_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109752.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9408..9659
                     /locus_tag="ACG8VH_001185"
     CDS             9408..9659
                     /locus_tag="ACG8VH_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109753.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9772..10014
                     /locus_tag="ACG8VH_001186"
                     /pseudo
     CDS             9772..10014
                     /locus_tag="ACG8VH_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10104..10343
                     /locus_tag="ACG8VH_001187"
     CDS             10104..10343
                     /locus_tag="ACG8VH_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109754.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10490..14773
                     /locus_tag="ACG8VH_001188"
     CDS             10490..14773
                     /locus_tag="ACG8VH_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFP9109755.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14872..15237
                     /locus_tag="ACG8VH_001189"
     CDS             14872..15237
                     /locus_tag="ACG8VH_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109756.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15239..15691
                     /locus_tag="ACG8VH_001190"
     CDS             15239..15691
                     /locus_tag="ACG8VH_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109757.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16140..17615
                     /locus_tag="ACG8VH_001191"
     CDS             16140..17615
                     /locus_tag="ACG8VH_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFP9109758.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17638..17949
                     /locus_tag="ACG8VH_001192"
     CDS             17638..17949
                     /locus_tag="ACG8VH_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109759.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18310..19566
                     /locus_tag="ACG8VH_001193"
     CDS             18310..19566
                     /locus_tag="ACG8VH_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9109760.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIVVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19563..20108
                     /locus_tag="ACG8VH_001194"
     CDS             19563..20108
                     /locus_tag="ACG8VH_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109761.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20109..20609
                     /locus_tag="ACG8VH_001195"
     CDS             20109..20609
                     /locus_tag="ACG8VH_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109762.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20968..21246
                     /locus_tag="ACG8VH_001196"
     CDS             20968..21246
                     /locus_tag="ACG8VH_001196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109763.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21266..21610
                     /locus_tag="ACG8VH_001197"
     CDS             21266..21610
                     /locus_tag="ACG8VH_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109764.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21629..22540
                     /locus_tag="ACG8VH_001198"
     CDS             21629..22540
                     /locus_tag="ACG8VH_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9109765.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22619..23611
                     /locus_tag="ACG8VH_001199"
     CDS             22619..23611
                     /locus_tag="ACG8VH_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9109766.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23608..23826
                     /locus_tag="ACG8VH_001200"
     CDS             23608..23826
                     /locus_tag="ACG8VH_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFP9109767.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            23839..24249
                     /locus_tag="ACG8VH_001201"
     CDS             23839..24249
                     /locus_tag="ACG8VH_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFP9109768.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            24301..26829
                     /locus_tag="ACG8VH_001202"
     CDS             24301..26829
                     /locus_tag="ACG8VH_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9109769.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            26845..29055
                     /locus_tag="ACG8VH_001203"
     CDS             26845..29055
                     /locus_tag="ACG8VH_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9109770.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            29063..30154
                     /locus_tag="ACG8VH_001204"
     CDS             29063..30154
                     /locus_tag="ACG8VH_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9109771.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            30175..30717
                     /locus_tag="ACG8VH_001205"
     CDS             30175..30717
                     /locus_tag="ACG8VH_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFP9109772.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            30912..31169
                     /locus_tag="ACG8VH_001206"
     CDS             30912..31169
                     /locus_tag="ACG8VH_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109773.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            31183..31515
                     /locus_tag="ACG8VH_001207"
     CDS             31183..31515
                     /locus_tag="ACG8VH_001207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109774.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            31560..32108
                     /locus_tag="ACG8VH_001208"
     CDS             31560..32108
                     /locus_tag="ACG8VH_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFP9109775.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            32115..>32439
                     /locus_tag="ACG8VH_001209"
     CDS             32115..>32439
                     /locus_tag="ACG8VH_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109776.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKI"
BASE COUNT        11101 a         5011 c         6881 g         9446 t
ORIGIN      
        1 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
       61 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
      121 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
      181 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
      241 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
      301 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
      361 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
      421 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
      481 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
      541 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
      601 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
      661 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
      721 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
      781 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
      841 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
      901 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
      961 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
     1021 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
     1081 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
     1141 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
     1201 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
     1261 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
     1321 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
     1381 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
     1441 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
     1501 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
     1561 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
     1621 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
     1681 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
     1741 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
     1801 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
     1861 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
     1921 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
     1981 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
     2041 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
     2101 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
     2161 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
     2221 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
     2281 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
     2341 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
     2401 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
     2461 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
     2521 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
     2581 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
     2641 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
     2701 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
     2761 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
     2821 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
     2881 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
     2941 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
     3001 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
     3061 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
     3121 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
     3181 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
     3241 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
     3301 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
     3361 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
     3421 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
     3481 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
     3541 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
     3601 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
     3661 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
     3721 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
     3781 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
     3841 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
     3901 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
     3961 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
     4021 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
     4081 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
     4141 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
     4201 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
     4261 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
     4321 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
     4381 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
     4441 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
     4501 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
     4561 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
     4621 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
     4681 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
     4741 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
     4801 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
     4861 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
     4921 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
     4981 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
     5041 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
     5101 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
     5161 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
     5221 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
     5281 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
     5341 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
     5401 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
     5461 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
     5521 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
     5581 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
     5641 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
     5701 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
     5761 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
     5821 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
     5881 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
     5941 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
     6001 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
     6061 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
     6121 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
     6181 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
     6241 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
     6301 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
     6361 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
     6421 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
     6481 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
     6541 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
     6601 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
     6661 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
     6721 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
     6781 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
     6841 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
     6901 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
     6961 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
     7021 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
     7081 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
     7141 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
     7201 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
     7261 ttcatggccg ttctgaaaat ggatgagcaa gatagtgtga aataacttat aatcaatcga
     7321 aaaacctatt aaataaacct ttttgagtag atgcaacact gaggattatc tgtataaaag
     7381 attttaaact gtcatttggt tatcacggcg atactaaaat taacaaaatt ggaatatttt
     7441 tcttgaagat ttttagctgt cagtatatga tgaaagcatc tcataggagg tgcttttttc
     7501 atggttgata attcaagtgg aagggttctt aaatctctaa gaaaagagaa aaaactatct
     7561 caaaaaaagt tagctgattt agcaggtatt tcacaaagta ctttagttaa atatgaaaaa
     7621 gggtcaagaa aaatccctaa agacgtagat aatactctaa gcaagattct gaatatagaa
     7681 actctaataa aagatgagga agataagatt gagattctca tcggtaaact aattgcatac
     7741 agagatatga ataagttact taacaaagag ttagctgata atataggaat tagtgaagta
     7801 ttactcagtt atgtgttaaa tagaaagaga aatccgtcaa aagaaatgca aaaaaaaatc
     7861 gatatttttc tattaagtaa tgaaaaagaa attttgaagg agattaatcg tgatagtgag
     7921 atatttagtt tatcaaaaga cgataaaatt gttatgggaa aaagaatacg agaagtccga
     7981 aagaaccggg aagagacgct tgaaaaattt ggtaaaaact ttaccatcta tacaggcaaa
     8041 aatgttataa gtagatggga aaaaggtatt aatattccag atattgaaaa actaatgaat
     8101 attgcctatc ttggtaaggt cactgttcct taccttatgt acggagaaga ttataagaat
     8161 atacttccaa aagatgaaag agttagcgat tttaaaaaga taaattcttt tagtatggga
     8221 ttaaggatga ggaaaattag gaaagattat tatctagaaa gggaggagtt tggaaagctt
     8281 ttttctcctt caatttcaaa atggtccata gataggtatg agaatggtag agatataccc
     8341 aatactaatc gaattatcca atatgcttat ataggaaatc tttctttaga atttttgatt
     8401 tacgggatat aaaaatagtt tacgtcgata cttattaagt tatcgacgtt ttttattata
     8461 gaaaaaattt ataaaaggag gtttcttgat atgataagaa acaaaaaggg tttgttgaaa
     8521 gttgctttac tggcagcttt tttctaagta actttttgac aagctcaatg gcaacacaag
     8581 cggaagaagt aatacaagaa ctaactgtta tgaataacag ctagataaaa tgggctatgt
     8641 atgttatcca acaatttagt aatcactaga agcctagcga aagttcgctt gataagttat
     8701 agatgagtat tgatttgtag tgtttccaaa tcaaaagggt acagagccac atgaaatatg
     8761 ttgtcctctg cagcgtcagg atgggaatcc ataccatgga gattcggaga tctgtgaggg
     8821 aatacttacg tggttaatat gtcagttgtg ttggacgtag taatggtcat agtgggtaga
     8881 ttgactgaat tcttatatga gaaaatgaaa ccaacaagaa gatagggtta tttatacaga
     8941 attggggatc ttttctggaa aagtaatcgg ataatgcgag attcgtacgg tcttttttaa
     9001 atttctctaa aaaaaacggc atcgaatggg ttgggtgatt gataagcaat gaatcataga
     9061 ctgatataga ataaaaaatg attctattta tctttttaca tctatcgttt tttcaagtag
     9121 ctgcaaatta tatcagcatt caacaatttt tgtatacaag ttctagtaaa aggatctgaa
     9181 gaaaaagttg gttgttgtga agataaaaaa agaatcaggt tttactgagt ttttcttcaa
     9241 aatttgtgtt attaggttag gacaaagata agaagaagta tgaagattta aaaaaggaat
     9301 gaggaaaaga tagtaatttc cgattattgg agttttttta tttataaaga agaagcgaat
     9361 aagttgtgat tcctagattt cgcgcgtgat taattaatga gcaaaaaatg cggaaagttt
     9421 tagtgtttaa tacacgagga caaagtttct taggtattga aggcacacgt gtaacggttg
     9481 atgggtggtg tgattgtttc gaggttggaa aacatgttga acttatggca tttactgagc
     9541 taaaagataa gaacaacgtg gaatttttcg agagtgatat tggttgggac gatcatgaag
     9601 aagtttatgg aaaatttatt ttgcggaagg gaaattctta tacgaatggg gaagcataag
     9661 tagcgatttg ttagagatca ttgataggat tgaaattgtc gggaatattt atgagaatct
     9721 agagttattt gaggtgatag aatgagtgat ggttttgaac aagtatcaat aattaaaaat
     9781 caagttcggg agattcttag aaaaaaaggc tatttggtag atagttattt tgaaggagat
     9841 tacgaaacat gggtaggtgt ctatgctcgt ccagagaata aacctaccta tttagatcct
     9901 acgacgtcag aagatgccta tttacaaaat aggtatcgag tagatggttt taagcaagac
     9961 tttgcggagt ggtttgaatg ggaaattgaa aacggagaag taaaagaaga ataagagtac
    10021 ttgttgtatc aaaataagcc agtagaacaa ccaaaccagt agctagtaat gacaataata
    10081 ctgaggaatc ggatcattat ggtatgattg atatttctct tccagaagat cttaagccag
    10141 atcctgtaac aattggcaat agtgtcatga tttttgattc attagatgaa gctatgtcat
    10201 atggttggaa agaaattaag gatgaaggta gtaattggta tggcatgaga tttggtgatg
    10261 gtgaagttgc caaaaaagct ggtattccaa ttgggaaatg gacagttgat ttccacgaag
    10321 caggttcttt ttttgttctg taattatagt gggagggtaa aatcattgat gaaatgtgtt
    10381 cagataagag cgccagataa aaatagaaga gacagttcac gtcgttatca cttttggtaa
    10441 cggcgttttg ttttattata taaaattctc aggaaaagga ggaattcttg tgtatgcaaa
    10501 aacgaaaatg aattggttga aagcactcgc attggtggct ttttctcaat tctgtttctt
    10561 ggtgggaact gctcaagcac atgcagaaga aacgacggct atacaaccta cgatggaagc
    10621 cattgcccaa gtcgcttctg aagtgacaga agtccaacca gcagaagccg gggtcccagc
    10681 tggtgaagcc actgttgaac aggcccctgt gacagaagcg ccaacggtca attccaaaac
    10741 gactctcgat cagacgattg gggaaacaca acacccaggg aatagtcctg gtacttcaca
    10801 accagttgaa gaaactgcct caattacaac ccctgcttca gaaacgtcca ctctagaagg
    10861 agaaaaagct gccgaagcaa cgccttctgt gcagccagag tcttctgaga aacaagcgga
    10921 agaaaagaaa cgaatagaag aaacagtgga acaaccagcg gctactgctg acaaagaagg
    10981 aaaaaatgag gaaaacgttg aaaaaacaat ctcttcaacc gcagctagtc aaaatcaacc
    11041 aacgaaacca aacaaagaat ctacggctag tattgaaaat caaaagaaaa aagagcagaa
    11101 cattccaaaa acaggtacac ctacactgtt agtaacagta ccaaccaatc gttcggttcg
    11161 tgttcgccgt gcaaccaatg cagctgtttt aggtggtcgg aaaattgatt ggggcgaaaa
    11221 tattggtgtt acttctagtc cgaacaatct aaacttcact ttaaattata tggttcgttt
    11281 tgagattaat ggacaagtgg cgttttgtat tgagcctggt gtctcagccg cagaaggtca
    11341 agtttataca gctactgctt tgaaaaacta tttgaaaaat tcaaatgtac ggaaaaaaac
    11401 aagcttgatc tcttatttag gatatgtcac aaataaagac aaatccgatg aacaatacat
    11461 tgcggcacaa tttatgattt gggaagcttt gggcacaaaa atcccaatca aaaatatccg
    11521 tgttgattat gatcgtcgaa aagcagaaat tcaacgtttg attaataact ttggtgaaaa
    11581 agcaactttt aataatcaaa cacatacggt aaaaattggc gaaacgttga aagtaaagaa
    11641 taaaaatgat tttttgaaat atgtgaaaga cattcaaaaa ccaaaaggtg tcaatgtatc
    11701 tattcacggc aatgaagtat ggattacagt cacaaaagag gcaccggata aagcaaaaat
    11761 tcaattgaat aaaattaaag ttcaaggtgc gccattagcc tatcgtcgtc caggagctca
    11821 aacaattggt gtgttacatc caacagaacc taatgcgtct gttattaatt tacaaattct
    11881 aaaacaagga aacattcgtg tgtttaaaga agatgcggaa actggtgtga aagcacaagg
    11941 tgcagcttct ttagaaaatg cggtctatgg tttgtatcaa gcagatggga aaaagattaa
    12001 agaaattacc ttgaaaaaaa ttaacggcaa agtacaggca gaaattaaag atcttgatcc
    12061 tggcaaatat gttttaaaag aaatcaaagc gccaaaaggt tatgtgcttt ctgatgaagc
    12121 aattgaaatt gttgttgaac ctggaaaaac tgtcaatgcg accgcaaaag atcaagtgat
    12181 caaaggaacc attgatttag tgaaagttgc taataaagat ttagtcgatt caacgaatcc
    12241 agataacaaa ccaaaattag ctggcattga aatcactttg acgtcaaaaa caactggcaa
    12301 agtggtgaaa accgttgtga cagacaatga tggttatgct acttttggta aaaataccgt
    12361 tgtctttgat acgtacattc tttctgaaac caaaggaaaa gaagggtata aactatttga
    12421 accacttgaa gtcacaattt ctgaaaatgg gcaaactttc cattatgttt tagaagataa
    12481 agtaattgaa caacgtctta aagtggtgaa agtggatcaa gaaacaggaa aagtgattcc
    12541 acttgctaat acagaattca agatttttga tactttagcc aatcgttatg tcacaatgga
    12601 gattccaaat gatacagaaa caacggatat ctttaaaacc aatgaaaaag gctttttcct
    12661 aactaacggt acacttactt atggaaaaga tcgctatcgt ttggaagaaa tcaaagcccc
    12721 cgtaaattac gttttgaatc aaaacccatt agtgttctcg attaccaatg atacagaacc
    12781 gatcaaagtg atccattttg cgaaccgtca agcacggaaa agtgtaaaat tattcaagta
    12841 tgaagaatgg aacaagaaga aaacgccgct cgcaggtgtg aactttacct tgtataacgg
    12901 taaaaatcaa gcaattggtg aatataccac tgatcaaaac ggggaaattt tagtgaaagg
    12961 tctagttgcc gatgactatt acttcttgga aaatgcccca ttagaaagtt tccaaccaaa
    13021 tacagataaa caagcattca cggttgcatt caaacaaaat gcgccagatg atggggaaac
    13081 gttagtggtt tctgtacgta attacttgat tccaccaaaa ctagaaacac aagcgtcaaa
    13141 taaagccgat ggtgaaaaag tggttgatcc attagaaacg atcgtcatta aagacagtgt
    13201 ttcttatacc aacttgtttg tcggaaaaga atacaccatt tcaggagtat tgatgaacaa
    13261 ggcaacgaat gcgccaattt tacaagatgg caaagaaatc acaagtacct tggcctttat
    13321 tgcagacaaa gtcaatggca caaaagacct tgaatttgtg gttaatgcta gtgttctgaa
    13381 aggtcaaacg atcgttgttt ttgaaaaact tttccgtgat ggcattgaag ttgcagcaca
    13441 tgcggatatc aacgatgaag cccaaagtgt gaaagtgaat aatccaaccg ttcataccac
    13501 ggctgtcttt gaagacggat tgacagtcgc agacccaaca ggattgttta ccttaaacga
    13561 taccgttgaa attaccgaga ttcttccagg aaaagcatac ttggtcaaag ggatcttaat
    13621 ggataaagca accggtgaac ccttgcttgt gcatggaaat acggtagaaa cgagtgttgg
    13681 ctttattgcc aaagcagaaa atgaaacggt gatcgtacca tttacctttg atttgacagg
    13741 tataaatggt cgtgaaatcg tcgtgtttga aagtcttttt agaaatgatg aagaactagc
    13801 aaaccataaa gacatcaacg acaaagggca aacggtacga gtaactaacc caagtgtacg
    13861 tacaacggct acacataaac ccactggctt gaaagaggtt aatccattaa cgaaagtcac
    13921 cattacagac gctgtgaaat accacgattt ggtcgttgga aaaacataca cagtcaacgg
    13981 tgttctaatg gacaaagtaa cggaaaaacc agtgctagta gatggaaaag aagtcacaag
    14041 taccttaacg tttgtggcag aaacaacgga tggtacagtc aatctagact ttattctaga
    14101 cgcacgtgcg ttgcgaggca aagaagtcgt ggtctttgaa gatctgttta gagaaggcgt
    14161 attattggct tctcacgcag atattaccga caatgaccaa accatcaaaa tcagtaatcc
    14221 aaccattacg acgaaagcga cgaataaaaa tggcggaaaa gagatcttgg ccttgccaaa
    14281 tcaaactgtg cttgaatggg tcaaagtaga cggtcttgcg attggtcaaa tctatcgctt
    14341 aattgttcaa ggctacttaa caccagacgg cacaccattt gaaggcactc atgcggaaaa
    14401 agtctttgcg gccgataaag aaatgatgga attcttattt gaattcttag tggacggtcg
    14461 caatcttgca ggaaaaggct tgagttttgc ggaatggctc caagcgaaaa cggaagatga
    14521 gaaagaaacg tttgaagaag tggcgaaaca caatgtagat ctaacaaaca aagatcaaac
    14581 ggttcaaatt gtggaagcgc caaaaccacc gcaaccagtg aaaccagaac aacgggttaa
    14641 accagctact gttgtaccaa tgttgccaaa aacaggtgaa caaacaagta gtatacttat
    14701 ggttcttgga ttagtagtat tagtcattgc tttaggtata acactttata aagtacgtaa
    14761 agccaaagat tagtcatgag gaacaagcgg ttaggatttt taaccgcttg tttgattgta
    14821 atgtagaaat atttagaatt tattttggtt tttaagagga ggaaaaactt tatggcagaa
    14881 aaagcattgc gtatgaagaa agagttttta ggtcaagtat ttttattggg ttcacgggaa
    14941 cctaatccac caaaggaaat taatggtgtg tctgggcgat atgttcaatt acaagcagag
    15001 tgttcgcgag aagaacagcc tgcttttttt gtttatctca cagctgaaga ggataaaaga
    15061 ttacgattac cagaaaaaga ggtgcgtatt tcatttgaag aagcttattt tgtttttcgt
    15121 aaagaacgag tgaatagtcg tgaaacaaat gatatgggac aaacagagcg tcgaacaact
    15181 ggctatcaag atgttggtgt ctttttaact ggtgtaaaaa ttggggaggt tttgtaagat
    15241 gggattaaaa tttccaaata attatgacat tacagaagca ttagatgaaa cttgtttagg
    15301 agccgtacgc tttggcgcat tagaagagtc tgatcgtgta tttgataatg aaacaggcga
    15361 ttatacggga gaaattcgat cacagcgagc acaattagtt tttggaaatc cagatgaacc
    15421 aaagattgca cgacgagtta aatttccagc agatgtagat ttgactggtt ttaaacgacg
    15481 tgatcctgtg aaattaattg atccagaata tcatgtggta tatattccag cacaagatgg
    15541 caatcgtgaa tattcagatt tagtgattta tgcgaaagga ttagagaaag tagaagctcc
    15601 aacaaataat cctaatatga aacaatcaca agaaaaaggt ttagatcaaa aacaaccaag
    15661 tgttgattct aaggaaaaag agaaaaaata aggttttttt gaaagaatag gaaggagtct
    15721 tcctattctt tttatctaag atagaaggaa ggggagattt tgaattcaga taaaaccaat
    15781 caaatacttt gagagattgt gcttgttatc aaaatatcca acaattgatt gagtattttg
    15841 gagacaatcc aacgattttt gtacaaacaa atgcatatat ttgtgcggtt attgggcagt
    15901 gtatgttttt acgtgatctt gttcgttttt cattaccgta ccagtggaaa caagatcatc
    15961 aggaagtgat ttggattcgt atagtacgaa tatgtaatat gtgtgcacac ccttataaaa
    16021 atcgattaaa tctttttcga ttatgttttt ttactaaatg tgatattcct gtattagttg
    16081 ctttttgtca aaagttttta gcacaggaaa agtaaaaaca gaaaggagga atgtgacaat
    16141 tgaaagctca ttttttcaag tatcgtggtc ggcgaattcg ctatttcaat cgttatttat
    16201 ggttttgcta ttatggtttc tttttgtttc catttttgat tctgttaggt tggtttagtt
    16261 ggaaagtatt ttatccacgt ttaagtacgt tttcgaattg gcaaatgtat cttattcctg
    16321 gaataagtat tgtgttgtat ttgttgctgg ttagtgggtt aattttaggg ttgatgcatt
    16381 ggtcgcgact aaaagaagga tattttgcct ctgtttattg gcgtcagtta cttgttcgaa
    16441 tgttaattga caatcggttt gtttatacaa agaaacaagt tagtgagaaa caaacaaggg
    16501 aaaaaatgag attaccaaaa gtttattttt atcaaaagaa acaagaatta gtggtttctt
    16561 ttcctttaga tggtgggcgt tttcacgaac gatttttaaa aatgggtcat ttgttatctg
    16621 aagtattttt gcgtgattta attcgtgaag aacgagaaaa agcccgaatc aattatgtat
    16681 tcctttcaga ttctggtcgg attcctattg atcaatgtat agcagaaaat ggacgtattc
    16741 atttaatgaa aactttggat tgggtatatg atcaatcacc aaatatgttg attgctggtg
    16801 caatcggtgg tgggaaaacc tatttattgt attctttaat tcaagcttgt ttaagtgttg
    16861 ggacagtaga tatttgcgat ggaaaagcgg cagatttagc agcattaggt gacgtcgatg
    16921 tattcaaagg acatgtgttt tacaaaataa atgaagaaat gattatttgt ttacgaaatg
    16981 cattaaaaga aatgaatcaa cggtatgcct atatgaaaac catgaaagat cctaaatatg
    17041 aaccagggaa aaattatgct tattatgggt taccaccgca ttttatcttt tttgatgaat
    17101 gggcagcttt ttatggttcg ttggatatgg aagcgagaaa tagagtcgat aaattgattc
    17161 agccattagt attaaagggt cgtcaagcag ggatgtacat tattttagca atgcaacgac
    17221 cagatgcaga atattttcca agtggtgttc gggataattt aacattccgt atttctgttg
    17281 gtcgattgtc tcccattggt tatttaatga cttttggaga agattttaaa aataagccat
    17341 tttacaacaa agatcaacct ggtcgtggat atgcttttcc aggatttgat gtacctgtag
    17401 agttttttgc gccatttgtt tacaaagatt atcactttat caaagaattt gagaaattta
    17461 ctgaaatgat tgtctatgat tttacagtaa aagaggatag tcaagatcaa acgatgggag
    17521 atgataatca gttgttacaa gaacaactag agcgccaaat gtatttagca aaagatccaa
    17581 aaggaacaag tttttctgat gcgcatattt tgtaatcaac taaggaggtg aagttaaatg
    17641 gcagagagtt gttattatag agtagctaca gcaatacgaa tgattaatcc ttctctttct
    17701 tccagaacgt tttatgattg gttgaatcgt attgaacaag taactgatta ccgctttttg
    17761 cgcaaagaac gtgtttttac aggaaaagtt attaatcaag tattgctaac gaaaaaagat
    17821 atagaacgat tgactcgatt gtatcattat cgagtagatt tagaagagga tttaactttg
    17881 tcaatttatc gcgttttttc tcctgaaaaa tatagtgaaa taacaaaatt agatcattta
    17941 attttgtagt tattatctat cgggagggct ctagacgtag tctggtagcc gcactctgcc
    18001 tggcggtgcg cattaaggct gcgaagcatg ggcctataat gcgcactcgc tagcagcgtg
    18061 tgcagctcac aaagaggtcg tgcgcttgcg cacggcctct ttgtgtaaag ccccccgata
    18121 tataatatgg ggggcagaaa taacataaca catagaaaaa agttttataa acccttttgt
    18181 atcaagtgat tatctaaggt cagcagttgt actacttttc ccaaaaaacg agggggacta
    18241 gttgatatat cggtgtttct aaagggttaa aaaaaatcga ttttgttatt ttataaaagg
    18301 agcgagaaaa tgacaccgat tgagctaaaa agctacagaa agaagttaag tgcaattcat
    18361 aatataagtg gagataaaaa gccgctatca caagcaagat ttgcgcaatt gatgggctgc
    18421 tcaaaagatt acattagaaa gatggaacaa gggaaaagaa aaatcccaga ggattttgaa
    18481 caacgaatca aggaacgctt tcgattgtat atgaaaagcg gaatagatct tcgagcaatg
    18541 attgattatt tgcgtctttc cttttttggg acagactaca aaacagtgat tcaacgtgta
    18601 ttacgaatgg atctgacaga atttgaaaca cgtccaacgg gcttgtatct ttttgactgt
    18661 ctttctgttc gtgggaatat ctgggtgttc tggcattcaa aacgaactgt aacagaaaat
    18721 gtacttgtgc aattttcagg acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa
    18781 caaacagatt ggcgaacatg gctatattcc atttggaaaa atcaaggaaa gttggaagct
    18841 ggttatacac gtgtgcaatg tagccgattc gatattgcgt tagatgaact ttggggattg
    18901 gaaacggaag aacatttcga tctctttgaa ttactagaaa aacaaaaagc aggtttatta
    18961 gaaatggact ttaagagatt ccagaatatt ggcggttgtt ttttagatga tggctatttt
    19021 cgaagtgaag gattatcttt gtattttgga tcgcgaaaga gtcctctctt ttttaatttt
    19081 tatgagaaac gttttgaaat tgcgaaccga gaaaaaattt cagaattaga agcattaacg
    19141 aaatatggta tttataatcg atatgagtta cggttagcca atgagaaagc aacacaagca
    19201 gtagaagcat ttattttagg tgattcaccg aaacgtttag gagagattgt cgttggttta
    19261 attaatgcaa aacttattgt ttgggataga ctggaaagtg atgcacggat tgttgataaa
    19321 cgatgggcaa aattatttcg ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa
    19381 ttctcattag atcgttcaga acgctggttt ttgaaacaag tagctccgac attaaagttg
    19441 ttacaagaag cagatcgtat tttaggaaga gactttgtac aagaagggat tgataatgcc
    19501 gaactaaatg acgagcaaca aaactatcta gaatggatgg gaacgtcagg agggaaaatt
    19561 gcatgaatgc atatacgaca acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg
    19621 aagcattaaa tgaaaagatg gttgatttgt cttattttcg ttttcgagtt ccttatattg
    19681 aacaagcggt aaaagctgga cgttatcagg aaaaagagaa ctggcaagaa attgcacgct
    19741 tattagaagt tcgtaaggga tacgaacaag aactcgaaga gttggaattt agccgaagga
    19801 aaggaaggct agaattcatt cgcttttatc gattttcatt gccaatacct gcgattttag
    19861 cggttaaaaa aggctgcgat aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca
    19921 gcgaaaaacc tcttatggaa gaaatttcat tggtgactgt cacttgggaa atgagtcgcc
    19981 agccaacaga agaacagaca tatctttctt tagaagagat tgagatagaa ttagaggaaa
    20041 ttggtcgcta tgccacttgt tctacctatt gtggatcggt cattagtatt gctggtgtga
    20101 ttgtctaaat gaaaaaaatt atgatttatg ttggtgcgta ttggtcacgt gatccaacag
    20161 tattagaaaa tccagaggcg gtacactatt gcttgcgcca attattctat ctgtataaag
    20221 aacgattaga atcgttgatt cgtcagttac catatactga cagacgttta gatgaacttc
    20281 ttctaaggta tccagcaatg tataaacgac gaaaaaatag attgttacca gaagagtatc
    20341 ccattgaaaa acatgaactt gaagggcgat ttgtggctta tttctatgat gatgttcgta
    20401 tgcgcttagt agaacaacga atggaaatag aaaatgatcg ctactatttt atcaattatt
    20461 gtaagaagcg taagtatcaa gtaactgatg atttttatga ttgtatttta caagatgggg
    20521 aagtgattct atccaaaatt gctccaagtt ttcgtgaact tgtgccgatt gattttttga
    20581 aaaaatgtca tttgcggatt cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa
    20641 gccaaatcgg tttctgcttt tttatttttt ttaggaagga gaagatggta tgtcactgga
    20701 atttttagga gagccaattg aaaaggaaga gttcgtttcg cattatatgg atttgtttat
    20761 tcaggtgata aaaaactttg tgcgattgat gaattttatc cacgcgaaca agactatcta
    20821 gccgctgaac gaataaaaat cagtttatta tgggaaaaat tcacagatga aaataaccga
    20881 ccgccagatt atcgctattt atcagattgt ttaacgaata atgtgctttc taaagaatac
    20941 ctatttcaca aacgtgagaa aaatcaaatg tcggttgaac atttttgtga ttgttatatt
    21001 gcattattgc gtagtcaaca gttattaaca gtagaacgat atggtgcaga gtttggttat
    21061 gtcatgcaag ctgagcgtca tagagctaca agatgctatg aaaaagaaga tcaagtgatt
    21121 gatggctatg aagaattatg tattcgaaac aatcgctatt tgcaacaacg tttatatgaa
    21181 caattgatga tggatttttc aaatctctat agagggtatg aatgttcgat gattcaagaa
    21241 aggtagaaga aaagggggaa tttttatgtg gcgattatta tttttggtgg tatgtggtat
    21301 ggcattgttc tatgtgtatc gtgtctatct ctatccacga atggaacgat ggacaagttg
    21361 gtatagagat cctttaaaac aacgaaaagg attgaagcgg tatcgttatg aattacttgt
    21421 tgaattttcg gatgggcgac aagcacagta ttattttttt gcaaactatc caaaatacaa
    21481 caccagtgaa ctattgattc aattattcaa aaataatcca tatgttgggt tagaagaaaa
    21541 cggacgggag ttcttttatc gttcagataa tattagtgcg gcgtctatga acaaaacacg
    21601 aattgcataa aaaattggag gaataatcat gaaaaaaaca acattagcag tcgtaacaac
    21661 actaggaatt tcattatttt cgtttcaatt agggagttca ttggtttatg cagatgaaac
    21721 tgtagaaaca cctaagtcag aaattgtaga aacattgcca ggggaaggat taccagatgt
    21781 tgagacagag ctaaatggag gaacaacgga aaaaccagca gaacctgaac aattgcagtc
    21841 agagaaacct gaaaaagagc cagtgacacc agaaccagag tttcctgaat caactttacc
    21901 gactgattcg atcgaagaag aaggtgtgca tagttcaaca acaacggatt caagtatagt
    21961 cacaactccg gaacaatcag gaacaaccag ttcttctgaa aagaatactg attcgtcaag
    22021 tacggaacag ccaacgatgt cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc
    22081 tgttgaaaaa tcagaagcaa ttccaaaaga aaataaacct attgttccag caaaagaagt
    22141 cacagtatcg gtaaccccaa gcggtgaaat tacgacgaat acttctcaag gaatgagtgt
    22201 accgattgtc actagtaatg tagaggaatt gacccatatt ccaacaccaa ccaccccatt
    22261 aaaagtagca ggtgatcaaa caattgttgg tgtaaaagat ggtattccat taatccaaga
    22321 tagtgaaggg aatttggtaa aggatttgtc gattcctgta aagaaacttc caagtggcaa
    22381 tattgaaatc aaaacagcag atggaaaaac aaaagtctta ccaaagactg gtgaagaaat
    22441 acatgttgca ctttctgttc taggaggagt gttgacaagt attgctggat ttgttggata
    22501 taaaagaaag agaaaaggaa tagaaaatga gggtaattga atggactatt tttcagaaaa
    22561 acaagcttat cgttgtcaat cgtacgtact agtaaaaaca tactagaagg agtagcttat
    22621 gaaaaaacga caactacggc ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa
    22681 gaaaatccat tcaaaaaaag cagtgaaatg gatttggtgt ttggttttat ttctttgttt
    22741 ttcagggggc ttggcatttt ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa
    22801 agatgaaatc caacaattac aaaagaaaga agtacaaagt cggtcagaat taacgtacac
    22861 acctgaaatt gaatccttta tgaatcgttt tgttgctttg tatatgaata cattcgataa
    22921 tcatgaacaa gaacaacaac gtcaagaaac attaaaaaaa gagttttatg cacaaaactt
    22981 tattcaagaa ccaggaagtg tttcaggtgt tcgaaaatta actgctgctt cgtttattgg
    23041 attaaaagaa attgaaacca ttccaactgc tagttataaa gtaacttatg atattcgctc
    23101 gatgaatcca gagagtaaga aagaaacaag tgtgacaatc acacaagtac tcaatcttcc
    23161 cttttctatt tcaaacaagg gctgtcgtat tattgcttat ccttatttta cggcactacc
    23221 aaagaataca gaaaaacaac gaatgattac ttatgatgac acattatacg aaacggttga
    23281 tgcgtcggaa gcgaaagatg ttgaaggatt tgtggaagat ttcttagata agtatgcgaa
    23341 aaacaatgca aaagatatgt catacatgat gaaagaacct gaaggactca atcaaacagc
    23401 taaagttgat caagtagaga tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat
    23461 tgtaaaagca acggtcgtct ttcgagatgg tgatacgtca cttttacaaa aagaacaaat
    23521 gacgattgtc attacgaaaa aaacaaatca gtattatgtt gaaaaattaa cacataattg
    23581 gttggatagg aaaggagaag aacggaaatg acactgaagg gaattgtaga ttttatatta
    23641 acgcaaggtg gctatttttt aataatggtg gttgtttatc tgggattgcg aaattttatg
    23701 aaatcaaaag taggaaatgc attaatatct atcgcagcag gtgccgcagt ttatttcttc
    23761 ttaaatgatc caacacgtgt atttgatgcg tttggtaaaa tcattgaaaa attatttatg
    23821 gattaggagg atagtgaagt ggattgggaa ccttatgatt attcgattgt ttatgcggaa
    23881 ccttatcgcg tacaacagat tacaagcaat tggcgtcttc catttccagt tgcattagtc
    23941 aatgcagcaa cttttttgtt ggtcgtaatc tttgaatatt tatttttacg ttcatttatt
    24001 ggttgggtca tttctttttt tccaccagca ggagttgttt tttatatagg actcccctat
    24061 ttatgtgtgt tagcactggc aaaactaaaa cctgatggaa aaaaagtgca tttttattta
    24121 tgggattggt tttgttatat gattcgtatg atgaatggtg attgtgtgta ttataactgg
    24181 gacaaggtat caaacaatgc attggaagaa ttactttttg tgaataagta tgtttattta
    24241 gaggattaga aaaagggaca atcaactggt tgttcctttt ttattgggag gtgaaattca
    24301 ttggtgcttg taaaagaacc agtaaaagca ttcaagaaaa attgtttgtt aaatggagag
    24361 gattgtatct gggcctacta tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa
    24421 aagacacagg aaataaaaga aatgatggct cgtttactgt tgcttttagc agattatgaa
    24481 gatgtggagt tggtgatgta tccacgaaca atgaatttac ggacgaagtt tgacacatta
    24541 ggaaagggat tttctcccga tagtcaagca attggtgcgt attatgcaaa agaaacagtg
    24601 acattgttgg aacgagaagg tcaagtctat cgtccttcat ttattttagg aatcaaagtc
    24661 aaagaaaaag caacggtgga gagttttcat gatgtaataa atctaatctg gttagattta
    24721 gcaggaagtt tagcacgctt atttggatta gaagtagatg atttggaaac tttgttttct
    24781 gaaagtgatc cttatgtaaa agaagtgaca caattacttt atagcttttc tattcgtccc
    24841 ttagtaaaaa aagagctgtt gtatctcaat cgtctgcaat atattcgagg aatggctcac
    24901 aaagtagaag aacaaaataa ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt
    24961 ataccgaagg aacataaagg ccttcttcaa ttagaagatg agttagggag tagttattta
    25021 gcattgttgc ctattgggga aacacctgaa aatatgagaa attgtgatct atttcaattt
    25081 gctcaggatc tttctttccc agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa
    25141 ggacagggag gtattggttt aaaactgaat tggctatcgg aacggttcaa aacagagcat
    25201 aaggaattgg ttcaaaatga tgaagaacct agtgaacgct tattaactgt tcgaggatta
    25261 gtaaaacatt taaagcaacg agtagataat gggcaacctg tcgtcgattg gatcgcatgt
    25321 tttattgtat acggtcaaac ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa
    25381 caacttcata cgattatcca tatccaccgt gcaaaaaatg atcaacgaga attgttctat
    25441 aaatgtttat tagggcaacc attgggacgg cagttacatt ggaagcaccg aacaactgta
    25501 gaagcagttg gcgaatggtt gttttcagca gtatcagaat taggtatgga atccggctgg
    25561 tatattggtc ggcaagatac attaggggat ggtggttctc gaggagaaaa aacaccatta
    25621 gaagaattaa tttatgcgtc aaatcgtttt gtctttttga atcttctagc cattgcggaa
    25681 ggaatcaaag gtgcgttgtt tgatgcgcct catgtagcag taactggaaa aaccggtaaa
    25741 gggaagacct ttttagttgg cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt
    25801 ttatttgtgg atcccaaagg agaaaagcgc aaatggttta cgaaactcat tcaagacccg
    25861 tattatcaga aacattaccc tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg
    25921 ttagatgcta caaatccccg taattatggt gtattagatc caattgtttg tttatcccga
    25981 gtagatgcga aacaagtagc ccaggatatg attgatgagt taagtccgtt aggtaccaat
    26041 caccttttaa aaggggcggt attaaaggga atccaagaag ttttagttca aaaagaagcc
    26101 ggacaacaag ttggcttgat gcatgtggtc gactatttag aacaaatgga acaaaaagag
    26161 gttcgagaat atggtgagtt tttacgcttg acggtagaag attcgatttt acgtttagga
    26221 ttttcttatg gagaaaaccc gggattagac tttaatgcca aaacaacgat tttagagatc
    26281 caagatttga aattaccaaa tgacaatgtc cgaccagaac tatatacaga tgcggatcga
    26341 aagtctttgt gtttgatgat tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat
    26401 agtagtaaga aaacagcgat ttattttaca gaagcatggg tatttaataa ttcgaacgca
    26461 ggacgtagta ttattgcagc aatggcacgt gtggggcgtt cccagatgaa tcaattagtg
    26521 ttagatacac aatttattgg tgacttaggt tcagataaag aaaaagggaa ttttggtgtc
    26581 gtctttgctt ttgatgaaga cagtgagcga gaaaagattt tgaatcattt acgtttggaa
    26641 gtaaatgaag cgaacttagc tgaaatgagc cacatgatta aaggacagtg ttggatgtta
    26701 gatccctatg gacgtgtagg aaaactcaat gtacacagtt tgtttgaaga aatgaccgct
    26761 gcattacaaa caacggaaaa aacaagtcac tctcaagtag aagaacagga tacgttaatc
    26821 atgcaataga aatgaggtga aacaatggct tatataaagc ataaggaaaa gaaatggtgc
    26881 gttttgtttt ttttaacgat tggattcgga ctgctttttg ttggtggatt ggtgtatgcg
    26941 gcagaaaagc aggtggatca atcgatctat gagattggga aatttcatat tgataccttt
    27001 tcttcttatt tatctgatag tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc
    27061 acagattttc taaaaatgat gacgaatatg ttctttagtt taagtaaatt gatttggcaa
    27121 ggccttgatt ttgtcattga gaagctctat gaaggagctg caatgaacga gttaattcac
    27181 cagtttttca ctttttcgga tactatttat tcaaaaatat acgatcctat tggtgcgggg
    27241 attgtcgtct tgtatgtgtt atatctattt actcttcgta tatttagagg gaaacattac
    27301 gcaaaaatgg tgtttattcg atttctcttc gttgtcattt tttcaatggt ttggtttggc
    27361 tatggtagta acgtacctag taaaggagaa gcttttacca aagggttaaa tgatttgtcc
    27421 gtggaggtag aaggctttat tttttctgct acaaatagtc tagatgaatt ggcgacaaaa
    27481 gatgtcaatg gtgttttagt gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa
    27541 ctttattata aagcggctgt tgttgatccg tatatattgt taaattatgg gacaacaaat
    27601 ttagaagaat tgtatcaagc agagattgaa ccacaagaat ttctagccaa ggaagcgtca
    27661 agtgaagaat tatctgatat tgagacaaaa gtgaccactg ctgcaggaga agatagaggg
    27721 aaaaagaagg cttatcgtgt ctatattcaa ccaactaaag ctgtttataa agcagtcgtt
    27781 ggttctatgg tgccattgct taatctaagt attggattac cacttgtagt gattggcatg
    27841 attcgtttta tctttcaaat gttggtgttg tgtttattac ttgtgattcc tttttcatta
    27901 ctcgcttctt ttgttccagt atttgattat atgctcatga ataccttaaa gtcctttttg
    27961 ggcttgatgt ttcaaaaatc tttttacagt gtgattattt tggtggcatt tttgatctat
    28021 aacatagtag acgatttaat tccaatgaat actgtttttg gttttttagg aaatatgttt
    28081 gtcaaagcac tcctatcaat tttactcatt gtaaaacgga aagatttatt aaagaaacta
    28141 gggttaggag ctgcagatca aactataaac aacacgaaaa acatagcaag tcattctttg
    28201 tctcaaatga aacaactgcc tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa
    28261 ggtgcaggat tagcagggaa tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata
    28321 catggatttc aaaagtttca acaaaaacgt acaccacaag aatcggtaaa agggaggcaa
    28381 gtggttaatc cctttgaact aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt
    28441 catgttcgtt atcatggggt agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg
    28501 aattctggtc tttcaaatgg tttgattcca aattcgtcca tgaattcagt aagaccaaaa
    28561 caacaggtac aatcattggc acaattgagt aagcaacaag ggaataatca aagaaaacca
    28621 ttgttaaata aaggtatcgc ttttgttggt caatcgattc cgttgccttc tggaaaagta
    28681 cccgtgccac aattaacagc tgctggttct ctttcacaac agattcgcca agtgaaaaat
    28741 caggcgaaaa atgggagtta tccagcaatg cagcagttat tgaaccaata ctctgttcct
    28801 aattcgtcgg tagccactct atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg
    28861 tctgaaagta aaccgtcgat ttccgcacca aaatcgttac aacctgcatt ttcttcttta
    28921 gataagccaa cagagcaatc ggaaaaaata gaaaaggatc ctaaaacact gccgattttg
    28981 gacgcaaaac caggaactga ttttacttta ccaaaaggac gaacagtcca aaaaccatta
    29041 tcgaagaaag gatgaggaag aaatgaagaa agggtgtcta ggttgtcttc ttttctttat
    29101 tcttcccttt tttctattgc cattcttttt ttcaggagaa ctttctgagc atggaggaga
    29161 agctacaggt ctagctgtcg cttttaatgg ggaatatacc gaaggattac cgattttttc
    29221 agagattaaa ggacgtggtc caatcacaga tgaacatgcg caagcagcag ttggtgcagc
    29281 tgtaaagtat cagttattac cgagtgtgat tctttctcaa ttaggctatg aatcagctta
    29341 tggacagtca caatctggaa gaaacgatac gaactttttt gggattacct ggtttcctgg
    29401 ttgtccctac ccacaaggtt ctgcacgtgg aattggtggt tctgaaggtg gtttctatat
    29461 gaagtttccg aacttaaaag cttgttatga ctattatggc tttatggtgg cgagccaaag
    29521 caactttaat caatgtgtgg gcaataaaga tcctggaagt tgtttattga ttctaggtcg
    29581 tggaggctat gcggcagcag gtattaccga agggagtccg tattatcgag gagcaatgag
    29641 tatcataacg tcctataact taacggaata tgatactttt gcgatcaagc attggaatag
    29701 cctaccaaca aaaaaaactt cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt
    29761 ggatcggatc attggacgat ccgtaaataa tggggaatgt tatggtggta cggcatggta
    29821 tgtatctcaa ttaggtggcc cacagttaat gggttctgga cacatgtttg cgatgttaat
    29881 aggggaagat tataactgga caacctatgg ttggcaagta atcatgaacc caaaaccaag
    29941 tgatttacaa gcaggagatg tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat
    30001 ctatggtcac accggaatta taacagatgt ttcatatggc ggacaacatt tctctagtgt
    30061 ggaacagaat gccgagcaag gacggattat ggctcggtat caacgcacgt acaatcaaac
    30121 aaaaattcgt agtctggttc gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa
    30181 aaagggataa ttttagtatt tagttggtta tgtttgatag gtcttactgg ttgtcattgg
    30241 ttttcttctg aaaaacaaaa aacacaattg agaacgacag aagaaacaat cgaagcagta
    30301 aaagaaacaa gtggaactct tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa
    30361 gacgagacag cgcaacaaat gcaaatagat gaaatgacaa aaaatttctt aaaagatttt
    30421 gctagaaaat ggagtaattt cacagatgtt tataaacgca atcaagctgt gagggactat
    30481 atgacagatc gttgtattaa agacaatagt attgatgtag acccgcatgt acaaatgaaa
    30541 acaactggga aactttacca ggtgacacaa gatatagaga atccggatca atatttgtta
    30601 tttggagaag aaacaatgaa taaggttact caatttgtat tacttcaatt gaaagtagat
    30661 caagaagctc aaaagattga tgcgatcaag atttattatg tacggagtgc gtattaaaat
    30721 aaaaaatgaa cactcatatt tattatacac cgaagaaata gaaaaaaaca gactgttttt
    30781 ttaagaaaaa ctatgtcatt ctttacttga aaaaagttgt tataggacag aaaggataaa
    30841 acaaaaacgg aaaaaatatg tcttatgaat aactataaga actattctat tttttaggag
    30901 atggatacga gatgaaaaaa ctaattggct atagtgtgat gtgtattgga tttgttggtg
    30961 tgggattgtt tgtatcgact gaatctgctt ctgcggctga atggtataat cctatctggt
    31021 ataatccgaa cattgaatgt caaaaaggct atccgtatgg accgacaaat ctgaaaaatg
    31081 gaaaatgtag agtaaattgg aatggcgttg tagatagtgt aattactggt tcaatggact
    31141 ctgctctaca tggctttacg ggtcactaga aggaggaaga aaatgtctga agagaaaaaa
    31201 gaaatcgttg aatcattagt agctttaaaa gattctcttt ctaagattga atatgttgat
    31261 aggcaagaaa ttcaaaaact aattgatgat actattattg aaatccaaga tgcgcgttgt
    31321 gaaggtataa aaatttctgt agctttatct aaagtaatag aaaaaatgaa ccgttcatta
    31381 gcgtttaatg gattgaagct agatagacaa actagtctga tatgggatca tttaaaagac
    31441 ttgtatgaca agtctaaaag aagtgaacgg actgctgtat caatcttaaa aggcctttgg
    31501 ggaatgaatt cataaacaag atatttaaaa agtaacttat tttttatgga ggagaaatta
    31561 tgaaaagaat aattaaaata tttggtgttt taggtgtttt cttattaggt ttaagtactt
    31621 ttgttagtac tggaacaagt gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa
    31681 taaagaataa attagtagat gaattaactt tttattttga tacagttggt tttttagacg
    31741 aacatggtgt ataccatata aaggattttg gtgctattcg tgaacgaatc agtcaagata
    31801 acgaacaagg aagagcagca agagtctttt atgaacatat tattgttcct agaatggaga
    31861 aagttgattg gaaacgatat gctggatgtg tagccattaa ttctcttcct ggaggaggag
    31921 tggcttgggc tataggtacg cagggagatt ttattgaagc attaaaagct tttaattttg
    31981 aaaaagctgc ggatttatta ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta
    32041 aagagttagc taaggtaaat attgtagcag gaattgcagc agctgctgct agctgtgcat
    32101 tagagtaaaa gtttatgaaa agtttagaac aagagcatat ggcttttaaa caagcaatgt
    32161 ttaaagaaaa tatctattta aaccataact atattcgtgt ttctaaggca tgttcaccag
    32221 tattgaatat gcttggaggt ggaaatggat tatatcattt actgtttgtc gatgtttgtt
    32281 ggttaatttt tttaccagat gaactagtta tagtcaatga aaaaataaca agtaaaaatg
    32341 aagtgtttaa ttattcttta acaagaatta attacaaaga gattaccaag ttttcactag
    32401 aaaaagttcc tttttgggga gaattttgtt taaaaatta
//
LOCUS       DAZKCC010000024        32322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000024 DAZKCC010000000
VERSION     DAZKCC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.024"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..801
                     /gene="truA"
                     /locus_tag="ACG8VH_001210"
     CDS             55..801
                     /gene="truA"
                     /locus_tag="ACG8VH_001210"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9109777.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(1256..3925)
                     /locus_tag="ACG8VH_001211"
     CDS             complement(1256..3925)
                     /locus_tag="ACG8VH_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP9109778.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(4163..4351)
                     /gene="rpmB"
                     /locus_tag="ACG8VH_001212"
     CDS             complement(4163..4351)
                     /gene="rpmB"
                     /locus_tag="ACG8VH_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9109779.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            4621..4983
                     /locus_tag="ACG8VH_001213"
     CDS             4621..4983
                     /locus_tag="ACG8VH_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9109780.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5035..6717
                     /locus_tag="ACG8VH_001214"
     CDS             5035..6717
                     /locus_tag="ACG8VH_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP9109781.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            6834..8870
                     /gene="recG"
                     /locus_tag="ACG8VH_001215"
     CDS             6834..8870
                     /gene="recG"
                     /locus_tag="ACG8VH_001215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9109782.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            8919..9920
                     /gene="plsX"
                     /locus_tag="ACG8VH_001216"
     CDS             8919..9920
                     /gene="plsX"
                     /locus_tag="ACG8VH_001216"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9109783.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            10042..10284
                     /gene="acpP"
                     /locus_tag="ACG8VH_001217"
     CDS             10042..10284
                     /gene="acpP"
                     /locus_tag="ACG8VH_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9109784.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            10552..11556
                     /locus_tag="ACG8VH_001218"
     CDS             10552..11556
                     /locus_tag="ACG8VH_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109785.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            11557..12501
                     /locus_tag="ACG8VH_001219"
     CDS             11557..12501
                     /locus_tag="ACG8VH_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109786.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            12501..13463
                     /gene="opp4B"
                     /locus_tag="ACG8VH_001220"
     CDS             12501..13463
                     /gene="opp4B"
                     /locus_tag="ACG8VH_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9109787.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            13490..14404
                     /locus_tag="ACG8VH_001221"
     CDS             13490..14404
                     /locus_tag="ACG8VH_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109788.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            14430..16211
                     /locus_tag="ACG8VH_001222"
     CDS             14430..16211
                     /locus_tag="ACG8VH_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9109789.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            16559..17245
                     /gene="rnc"
                     /locus_tag="ACG8VH_001223"
     CDS             16559..17245
                     /gene="rnc"
                     /locus_tag="ACG8VH_001223"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9109790.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            17265..20846
                     /gene="smc"
                     /locus_tag="ACG8VH_001224"
     CDS             17265..20846
                     /gene="smc"
                     /locus_tag="ACG8VH_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP9109791.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            20855..21664
                     /locus_tag="ACG8VH_001225"
     CDS             20855..21664
                     /locus_tag="ACG8VH_001225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9109792.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            21675..22673
                     /gene="ftsY"
                     /locus_tag="ACG8VH_001226"
     CDS             21675..22673
                     /gene="ftsY"
                     /locus_tag="ACG8VH_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9109793.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            22860..25127
                     /gene="gshAB"
                     /locus_tag="ACG8VH_001227"
     CDS             22860..25127
                     /gene="gshAB"
                     /locus_tag="ACG8VH_001227"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP9109794.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(25216..25668)
                     /locus_tag="ACG8VH_001228"
     CDS             complement(25216..25668)
                     /locus_tag="ACG8VH_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP9109795.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            25818..26768
                     /locus_tag="ACG8VH_001229"
     CDS             25818..26768
                     /locus_tag="ACG8VH_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9109796.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            26761..27720
                     /locus_tag="ACG8VH_001230"
     CDS             26761..27720
                     /locus_tag="ACG8VH_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9109797.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            27717..28472
                     /locus_tag="ACG8VH_001231"
     CDS             27717..28472
                     /locus_tag="ACG8VH_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109798.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            28495..29448
                     /locus_tag="ACG8VH_001232"
     CDS             28495..29448
                     /locus_tag="ACG8VH_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9109799.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(30092..30388)
                     /locus_tag="ACG8VH_001233"
     CDS             complement(30092..30388)
                     /locus_tag="ACG8VH_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP9109800.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            30743..31099
                     /locus_tag="ACG8VH_001234"
     CDS             30743..31099
                     /locus_tag="ACG8VH_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9109801.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            31109..32008
                     /locus_tag="ACG8VH_001235"
     CDS             31109..32008
                     /locus_tag="ACG8VH_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9109802.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        10708 a         5732 c         6906 g         8976 t
ORIGIN      
        1 ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta
       61 cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca
      121 aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa
      181 gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta
      241 atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc
      301 cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca
      361 agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt
      421 ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag
      481 cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc
      541 tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa
      601 gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta
      661 gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt
      721 attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg
      781 tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa
      841 ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc
      901 tacttctgga acaccgttta aggaagaggt cgtgaaatca gcgttttgac ctgagtatta
      961 gagaaacaaa cgggaaaata gctcctcata ttttgccgtt tgttgagcta ataccgaagg
     1021 tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca
     1081 caaagagcaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga
     1141 ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg
     1201 aacgttgttt tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt
     1261 ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc
     1321 cataaaaggg gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata
     1381 ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat
     1441 gtttacctgc tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg
     1501 gtttcccatc aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg
     1561 aatcttcagc ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac
     1621 ctttttcttt gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt
     1681 taggctccat gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg
     1741 tctcttgagg ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc
     1801 gcgatgtacc attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg
     1861 ggaacaaact taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt
     1921 ttggctggtt atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg
     1981 tattcaactg attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag
     2041 acaatgtatt gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat
     2101 cattaacgga caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg
     2161 tatagtcgcc gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt
     2221 tagaaatgtt atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac
     2281 ggatattcaa atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat
     2341 tacgatttct tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt
     2401 ttgcagatac tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt
     2461 catttacaag agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat
     2521 tccaatcatc aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca
     2581 tgatcaataa taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc
     2641 ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca
     2701 cataggtgta gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga
     2761 ccaaaatgat tcctaacaac agcccacgat cgtcttgttc tatcttttcc catccttttc
     2821 ctgcaagcag gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg
     2881 gtgcatgcat accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta
     2941 tggcgaaaag actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat
     3001 aaaacagaca gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt
     3061 ttgtcgtgtc ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt
     3121 ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg
     3181 ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa
     3241 caatcgtttt tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag
     3301 aaaatacgcc aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga
     3361 taaataaaag tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg
     3421 gaagataaaa aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga
     3481 tcaatgcgta actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg
     3541 caagaaacca aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg
     3601 catctggcat catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat
     3661 agtaagaaat taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac
     3721 tttgctttcc aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg
     3781 catcacttgc caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa
     3841 gtgctaaaat caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa
     3901 agaatgactt taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt
     3961 cagtttatca tatccttgtt tttctctgct taaaaagagt ctagttttca caaaaaaata
     4021 aaatttagaa taaataactt cataatttgt tcgtataaca aaaataaacg caaaaaaagg
     4081 gtaggaatca aaagtcccac ccttaaaaat ttttattaaa taaaaaaccg ttcactatct
     4141 gaacggtcca agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag
     4201 ttgacaccca aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag
     4261 gtttaacagt acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct
     4321 tgcgacctgt aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga
     4381 gcaatagaat aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag
     4441 tacattttca tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat
     4501 acctgaaatt ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg
     4561 ctatgataaa atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc
     4621 atggctgtaa aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca
     4681 acagttgttg gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat
     4741 caaattaaag ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt
     4801 gtccgccaag aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg
     4861 aaaatctctg aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta
     4921 ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac
     4981 taggcaggag actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt
     5041 gtaacagaaa taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg
     5101 caagtaaatg cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg
     5161 ggaacaaata tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca
     5221 gaaaaagtag gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga
     5281 aattccggcg ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt
     5341 gtctcattaa atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat
     5401 aaagcagtaa tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa
     5461 gctggagaaa agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta
     5521 aaaggagcaa agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc
     5581 ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca
     5641 ttgaatggag aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat
     5701 atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat
     5761 tttggttact gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca
     5821 ttcgattacg aagaattccg taactattta gatggtatag gagattcttt acttgtggtc
     5881 aatgatgacg agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat
     5941 tatggacaaa aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat
     6001 gagacgattt tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt
     6061 atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt
     6121 ttaggcgcta attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt
     6181 gttaaagcga ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac
     6241 atcttcatgg cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct
     6301 tcaagaacga tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta
     6361 gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact
     6421 cacgcgatca gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc
     6481 atgatcgatg gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca
     6541 ctagaacaaa tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga
     6601 acagttgaac aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa
     6661 atcgagatcc accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt
     6721 ctgtgtcaga atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc
     6781 aggtatgatc gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt
     6841 ctttagatga tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg
     6901 ccagcttggg catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag
     6961 atattcagga aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa
     7021 tcgtagtctc gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc
     7081 gtatgatgca agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag
     7141 aaaaagtcat tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag
     7201 ccttgaacgg gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc
     7261 acgtcaacaa atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag
     7321 aatacggcac attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc
     7381 ttgaccgagc ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc
     7441 aagccaaacg acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat
     7501 tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat
     7561 tgaaacaatt tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga
     7621 atgaaatctg tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg
     7681 taggtagcgg aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt
     7741 tccaaggtgc gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc
     7801 aacagctgtt tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa
     7861 aagaacgacg tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca
     7921 cacatgcatt gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg
     7981 agcagcaccg ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag
     8041 atgtcttatt tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg
     8101 agatggatgt atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct
     8161 gggtaagaac acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag
     8221 gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa
     8281 atgcagtgga aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct
     8341 tgttacatgg taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca
     8401 accagatgca gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg
     8461 cgaccgtcat gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc
     8521 gtggacgtgt tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa
     8581 atgaactagg tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa
     8641 gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc
     8701 tgcctcaatt tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag
     8761 aagaggctgc agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt
     8821 tagcgcaaag cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc
     8881 tatactttat actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa
     8941 tgggcggaga tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt
     9001 ttccagaagt agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa
     9061 atgaaaaaaa catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag
     9121 taaaagcaat tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa
     9181 acggtgaagc agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt
     9241 tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag
     9301 tagtagggaa agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca
     9361 aaccagaaca tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag
     9421 gtatcggacg tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg
     9481 aagtgacgaa aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa
     9541 acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta
     9601 caggcaacgc tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa
     9661 aatctgctat tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg
     9721 gcttacgttc tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg
     9781 gcttgaaagc accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata
     9841 cgatccgcca aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat
     9901 ttgaaaaaag cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag
     9961 gtggacattc ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta
    10021 tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt
    10081 atttcaaatc attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat
    10141 gatttaaaag ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc
    10201 ggtacggaaa tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat
    10261 tatatcagca gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat
    10321 gcagcttttt atccttcttt ttttaatctt tttgacacgt aaaagtcatt tttctgataa
    10381 ttctggcgat aaacagaaat tatgttgcaa agtaaagcgg ttttctatat aattattaat
    10441 aattcattac acaaagataa gaataacctt tgttataaaa cgatttattc tgtgtaatgt
    10501 ccttttttat taagaaaaac ttccatttag gaagcagaga ggggtattcg attgtccgat
    10561 aaccaattat tagaagttca gaacctacat acaggattcc gactcaaaga tgactattac
    10621 gatgcagtag atgacgtttc atttacttta gacaaaaacg agattttagc aatcgttggg
    10681 gaatctgggt gcgggaaaag taccttggct actacgatta tgggattact tgatcctaat
    10741 aacacaaaaa ttacaggtga gattttatat aacgatctga atttaatcga cctgaacgaa
    10801 acgctctata ataaaatccg cggaaatgat atcggaatga ttttccaaga tccgttgtct
    10861 gcattaaatc ctctcatgag aatcgaagac cagatcaaag aaggactttc ctatcacaca
    10921 aagatgaatg cagaacagcg tcaagctcgt gcactagaat tgctagaaca agtagggatt
    10981 cctaatcctg cgagagttgg acgccaatat ccccatgaac tatcaggcgg tatgagacaa
    11041 cgtgtcatca tcgcaatcgc aatcgcctgc aaacctccaa tcgtcattgc agatgaaccg
    11101 acgacagccc tagatgtaac catccaagca cagattcttg atttactgaa agaccttcag
    11161 gaagaaacaa aaacagggat catcttgatc actcatgact taggagtcgt agcagaaatg
    11221 gctgatcgag tggcagtgat gtatggcgga cagttcgttg aagtagcagg tgtaaaagaa
    11281 ctgtttgatc atcctaagca tccttataca cgctctttac tgaactctat accacaagaa
    11341 ggaaatcatg atgcagaact ccatgtgatc gaaggtgtcg ttccttctct gaaaaacatg
    11401 cctcgtaaag gttgtcggtt tgcaccacgt attccatgga taccagcaag cgatcatgaa
    11461 gaagagccta tccttcatga agtcgcaccg aaccacttgg tacgttgctc ctgctataag
    11521 catttccact ttagagacga aaaaggagag gtgtagatga cagaattaat cacaatcaaa
    11581 gacctgaaag ttcattaccc tattcgcagt ggattcttca atcgagtaac ggatcatgta
    11641 tatgctgtag atggtgtgga ttttattatc gaaaaaggaa agacatatgg tttagtggga
    11701 gagtctggct ctggaaaatc tactacggga aaagccgttg tgggattaga aaaggtaaca
    11761 gccggacaaa tcatgtatga aggaaaagat gtaacgaagc gaagcaaccg caaaaaaatg
    11821 ggttataaca aggatgtgca gatgatcttc caagattcaa tgtccagttt gaatcctaaa
    11881 aagcgagtgt tggatatcat tgctgaaccc atccgaaatt ttgaacggtt aagcgatcaa
    11941 gaagaaaaga aaaaggtaaa aggactgcta gatatcgttg ggatgccgga agatgcgcta
    12001 tataaatatc ctcatgaatt ttcaggcgga caacgtcaac gtctaggagt agcccgtgct
    12061 gttgctacca atccaaaact gatcgtagcc gatgaacctg tatctgctct ggatctatct
    12121 gttcaagcgc aagtattgaa tttcatgaaa cgaatccagc aagaatacgg cctcagctat
    12181 ttattcattt cacatgattt aggtgtagta aaacacatgt gcgacaatat cgcaattatg
    12241 tacaaaggtc gatttgtaga aatcggtaca agagaagaca tttataatga cccgcgacat
    12301 atctatacga aacgtctgct ttcagctatt ccgcaaatcg atgtagaaaa tagggaaagc
    12361 cacaaagaaa atcgacgaag agtagaacaa gagtatatcc aaaaccaaaa agaatactat
    12421 gatgcgactg gtcgagtcta cgatttgcgt acgatcacaa ctacacacaa agtggcgttg
    12481 aaagatggag gtgcaagcta atgtggaaaa cgattctgcg acgtgtttta ttgatgatcc
    12541 cgcaagttat tattttaagt gttctgatct tcttattagc ccaagcgatg ccgggggatc
    12601 catttaccgg tttgatcaat ccaaatcagg atccggcagt gatcgaacag atgcgacaag
    12661 cagctggctt gaatgaccca tggtatgtcc agtacttccg ttggatcgga aatgctttgc
    12721 aaggcgattt tggtcaaagt ttcttataca aacgctctgt tgctgacttg atcggcgaac
    12781 ggatcatcaa cactttatat ctatcgatct tgacagttgt ccttacttat ttgattgctg
    12841 tgcctctggg gatgcttgct ggccggtatc aaaattcaat cttggataag attgtcgtaa
    12901 tctacaactt ctttagtttt gctgttcctt tatttatctt cggcttgatc atgctatttg
    12961 tttttggtta tcgtttaggc tggttcccaa caagtggatc acaaacacta ggtttgcaag
    13021 gtggatttat cactcaatgg atagaccgtt tgaaatatat tctgttacca tcaattaccc
    13081 aagcattttt ggcgactgcc gtcacaatcc aatatctgcg aagtgaagta atcgattcga
    13141 aatcgctcga ttttgtcaga acagcccgtt ccaaaggggt accgacgaat aaagtattca
    13201 gtcgacatat tttccgaaat gcagcgttgc cgatggcttc tcaaatgggg tatgaaatca
    13261 cttcgttgat tgctggttct gtagttatcg aaaagatttt tggttatccg ggagtcggaa
    13321 aactatttat tgattccatc ggccaacggg actataccgt gatcacatcc ttagtattga
    13381 ttttaggaat cgcgacgcta gtggggacat tactttcaga tatcatcatg agtatcgttg
    13441 acccgcgtat tcggattcaa taaaaccaat cagaaaggag aatggatcga tgagtgaaaa
    13501 aaacaataca gcagttcaag aaagtattcc tcctatgggg atacgaatga ttgctcgaga
    13561 atttaaaaaa gataaaattg ctattttttc attaggtttg ctagtggttt tattattagt
    13621 gatctttatc ggtgcgatgc tgaccgatca agacaaagtg atgtatgtta gtatcttcga
    13681 caaatacgct gcaccaggaa cagtttcgac acaaggacaa aagttcattc tgggagcaga
    13741 tgaaggcgga cgtgatgtat taggacaatt gatcataggt gctagaaact ctgttacgat
    13801 cggatttgcg atcacgatcc ttacttcgat catcggggtt ggattaggga tcatctctgg
    13861 ctactacgga gggatcattg acaacatctt gatgcggatc gttgatttta tcatgattct
    13921 accgatcatg ctgatcatta tcgtcttcgt atcgattatt tccagataca atgtctggtc
    13981 ctttatattc atcatgagtg cattttattg ggtagctaaa gcccgattat tccgaagcaa
    14041 gacattatct gaagcaagaa gagattatgt cagtgcctca aaaacaatgg gaaccaatga
    14101 ttttaaaatc atgttccgcg aaatcatgcc gaatctaagt tcattaatca ttactaattt
    14161 gacgatgaac tttgcggcaa acatcggtat cgagacaact ttgactttct taggtttcgg
    14221 cttgccggca aatgtaccta gtttaggtac attgatcggt tatgcaagta acggagaagt
    14281 cttagtgaat cggcaatgga tctggttacc cgcatcaatc cttattttag ttttgatgtt
    14341 gagtatcaat tatgttggac aagcattcaa gcgttctgca gatgcgcgcc aacggttagg
    14401 ctagtataga agcgaagggg gaaataggaa tgaagaaaac gatttttgga tttgcagcgc
    14461 tgatgtctgc tgtcgttctt gcagcatgcg ggggcggagg tacagatacc tctggaaaca
    14521 actctggagg caacggcaat aacaaagagg aattaacttt tccaattgca acaaaaaata
    14581 atgatgatgc aatcgaagac ggcgaattag aagctgcggt cgcaacggac agtcagttta
    14641 aaggattgtt ccagtgggag ttttaccaag attcatatga tgcagctttt atggctcctt
    14701 ctcatgaacc gctatttgca aacgatgacg atttcagaat cacagacgac ggagctgcaa
    14761 cgcttgatct agatgaagat acgaaaaaag cgacaatcaa agtaaaagac aacgtcaaat
    14821 ggtcagatgg tgaagatgta acggcggacg atgtgatttt cccttacgaa atcattggga
    14881 ataaagatta cactggtatc cgttatgacg acactttccg taacatcgta ggtatggaag
    14941 aatatcatga tggaagtgcc gacacaatct ccggaatcaa aaaaatagac gacaaaacag
    15001 ttgaaatcga atataaagaa gtaaacccaa gcatgcttca atcgggtggt ggtatctggt
    15061 catatgcagc accaaaacat acgttagaag gtgtagcaat caaagatatg gaatcaagcg
    15121 atcaagtacg taaaaatcca gtgacttttg gcccttatta catgagtaat atcgttgcag
    15181 gagaatcagt agaatacttg ccaaacgagc actactggaa tggtacgcct aaattgaaaa
    15241 agatcacgat gaaatctgta ccgatggcaa gtgcaactga agcattgaac tctaaattat
    15301 atgatatgat tttccaaatg ccaacagata cttacgatac ctataaagaa attgctggct
    15361 atacaaactt aggccgtgaa caaacttctt atacttatct aggattcaag ttaggtaaat
    15421 ggaatgcaga tacaaagagt gtggaataca atccagattc aaaaatggct gataaatcat
    15481 tacgtcaagc aatgggctat gcattagata ataatgcagt aggtgaacgt ttctatgcag
    15541 gtttgagaag caatgcgact tcattgattc ctccagtatt ccagtcatac catgatgatg
    15601 agatgaaagg attcacacaa gatctagaca aagcgaaaaa actattagat gatgctggct
    15661 acaaagatgt ggatgacgat ggtttaagag aagacaaaga tggaaataaa ctaacaatca
    15721 actttgcttc gatgtccggt ggggaaacag cgcaaccatt ggcagattac tatatccaac
    15781 aatggaaaga aattggtttg gatgtccaat acacaacagg tcgtctgatc gaattcaatt
    15841 ctttctatga taaattagaa aacgatgatc ctgaaattga tgtttaccaa gctgcttgga
    15901 atacaggaac tgatccaaat ccaagtggac tatggggacc aaatgctgcg ttcaactaca
    15961 ctcgttatga atcagaagaa aatacaaaat tgatcaacga tatcagttca agcaaagcat
    16021 ttgatgaaga gtaccaaaaa gaagcatatc aaaaatggca acaatatgca tttgaagaag
    16081 catttgcgat tccaacgttg ttccgtaatg aagtattgcc agtcaacgac cgtgtcaaag
    16141 actgggattg gtcttatgat gtaccaaacc catgggcaaa tgtttctgtt acagcagatt
    16201 cacgttcata atcactaaaa atgaccgaaa aagggcgatg tcctttttcg gttttttgat
    16261 atttttctgt ttatttctct aatattcagg tcaaggcgct gatttcatga cttctccaat
    16321 aaacggtgtt caaagtccag tttctcggta ttaagtcaaa tttatgcaaa aatatggaaa
    16381 gccattttca caaaattttt tcttcatact cgaaactaaa cgaatttttc acaacttctc
    16441 gtcaatcact aggattatta aagctttact taccttttga ccgaaccatt tcaaatttga
    16501 caaaaaacca gtataataag gaagtatcga aaaaatatcc ataaaaggaa tgtggaaaat
    16561 ggacaaccag ctgacaaaag aactaaaaga aaactataac atcgtttttc acgatctaaa
    16621 tttgcttgaa caagctttta cccattcatc ctatgtgaat gagcatcgca atctgcaatt
    16681 atcggataat gagcgacttg aatttttagg agatgccgtt ttagaactga tggtttctca
    16741 gtatttattc cgtttatatc ctcatttgcc agaaggaaaa ttgacaaaaa cacgagctgc
    16801 gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa tgtcactttg atcaatatgt
    16861 tttgttgggc aagggagaag agaattcagg aggacgtacg cgaccagcat tgctttgcga
    16921 cctattcgaa gcttttttag gcgcgttata tcttgaccag ggattcgaca cgacccatgc
    16981 attcatcgaa aaagtgattt ttcctaaagt caaagcaggt gctttttcac atgagatgga
    17041 tcataaaaca aaactacaag aagtcttgca aaaatcaggt gatgtatcaa tcgaataccg
    17101 attgatcaat gaagaaggtc cagctcatga acgagtattt tggatcgaag tttatgtaga
    17161 tgatcaattg attggcactg gacaagggaa atcgaaaaaa ttagcagaac aagctgcggc
    17221 ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa agctttgtat ttgaaacgaa
    17281 ttgaaatagc cggttttaaa tcatttgcag acaagacagt cattgatttt gaaaatagcg
    17341 tcacagcagt ggttggacca aatggcagcg gaaaaagcaa tattacagag gctatccgct
    17401 gggtattagg agaacaatct gctaaaagtc tgcgaggcgg aaaaatgccg gatatcatct
    17461 ttgccggttc cgatacccgt aagcagctga atattgcaga agtcacagtg attttagaca
    17521 atagtgatca ttatctgcca ttggagtaca atgagatcag tgtaacccgc cgttatcgac
    17581 ggactgggga aagcgagttt ttcatcaata aacaaagctg cagattaaaa gatatccaag
    17641 aattgttctt agattctgga ttaggaaaag aatcgttctc aatcatttct caaggaaaag
    17701 tagaagcgat tttcagtagt aaaccggaag accgtcgtgg gatctttgaa gaagcagctg
    17761 gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca gaagcttttt gaaacagaag
    17821 acaacctcag ccgcgtccaa gacatcatcc acgaattgga agaacagtta actcctttgg
    17881 ccgcccagag tgaggcagca aaggagtttt tacggctaaa agaaacactg actcaaaccg
    17941 atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa agattgggac aacaaacaag
    18001 cacagcttgc gaaattcaat ctagaattag gcaaactatc tgagtcgatc caagaacaag
    18061 aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc cgatcgtctg atcgaaaaaa
    18121 atcagcaagt attattagat ctttcggaaa aactgaagca gacagaagga caaaaagatg
    18181 ttttgcagga gcggacaaag catacacaaa aaagctcgca agaatatcaa acttcattag
    18241 cagaagcaca aaaaaaggtt aaacattttg aaaagcttca agaaagcttg atgaaagcag
    18301 cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa tctcataaaa acacagcaag
    18361 aattagaaaa ataccaaaaa tcaacgaaag aattattggc agaattaaga gatcaatatg
    18421 ttgatttgat gcaagaacaa gcagctgtag gaaacgaact gaagtatcta gaacgtcaat
    18481 atatacaaga aacagcaaaa agcaagcaga cgttagccaa gcaatcggaa gtagaagcat
    18541 cagtagaccg attgatctta caaaaagaag aattgacaca aaagcaggcg cagctaaaat
    18601 catctttaac tgaaacaaaa gagaagttag aaatgatcca acaaaacggg aaaaagttcc
    18661 aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt aatgaatcaa gtccagcagc
    18721 tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa ctattttggt ttttaccaag
    18781 gggttcgatt ggtcttgcag cataaacagc agctttcggg tattgtcgga gcagtggctg
    18841 aactgatcga tgttcctgca gatttcacgt tagctatcga agcagcttta ggtggggcag
    18901 cccagcatgt catcgttgaa aatgaaaaag atgctcggca agccatcacg tatttgaaac
    18961 agcaaagagg aggaagagca acattccttc cactgacgac aatcaaacca agacaattac
    19021 cggctcatat attgacacaa gcagcagctg tagaagggtt tatagggatt gccagtgaac
    19081 aagtcagtta tcccgatcaa atccaaactg tggttcaaaa cctgctagga acgatccttt
    19141 tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac gatccgctat caatatcgag
    19201 tggtttcctt agaaggtgat gtgatgaatg ccggaggatc catgacaggt ggagcgaata
    19261 aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga attgaaacag ttgactagtg
    19321 aatttgaaga agcagataaa caactccaag cacaagaaaa aaaagttcag gagctgcaac
    19381 aagaaacagc tcgtttagcc gaagaacaag aagttttgcg tacacgaggc gaacaattac
    19441 gttttgaaga acaagaagcg acaaatcagc tacaaaacat cataaatgaa cttgaacgtt
    19501 ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga actgcagcag tttatcgaag
    19561 attacgaaaa gcaaacgaac gaattgaaag acaaacaaac agatcttgaa tcgcaacgac
    19621 aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag cgaccagatg gaagccagaa
    19681 gagcacaagt acaaagtcaa aaagcacaag aacaagcaga tcttgctgtc ttgaaagagc
    19741 agtttaatca tctgcagatc cagctccgcg gtgcccgagt ccaaaaagca gaggcaaccg
    19801 aacgtcaaga agcgatagaa aagcagttgg cgacattgac agcagacttt tctgatcatg
    19861 aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt atctgctcag cgagaaacat
    19921 taaaagccga gttggcaaaa gccaaagagc agcgagatag aaggcaaaag gagatcgata
    19981 agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa agcacgactg tcggaacaat
    20041 ccaaattaga agtccaaaaa gaccgagctg aaatgatttt agataatcat ttgacctatc
    20101 tgcagtcaga atatcagctg acatttgaga aagcttccca agactatcaa gaaacaaccg
    20161 atatcgaaga cagtagaaca aaggtttcat ccttaaaaga acagattgaa aaactgggac
    20221 cagtaaactt aaattcaatc gaacaatacg aacaagtcag tgaaagacat acattcttag
    20281 caacacagcg agatgatctg ttagcagcga aaaaccaatt gtttgaaacg atggatgaga
    20341 tggatgatga agtgcggaca agattcaaag aagtatttga agctatccgt caagaattca
    20401 aaattgtctt tccaaatatg tttggcggcg gtaaagctga attagtactg acggaaccaa
    20461 ctgatttgct gaaaacaggt attgagattg aagtacaacc tcctggcaaa aaactgcaaa
    20521 gtctaagttt gctttctgga ggagaacgag cacttacagc catcgcgttg ctcttttcca
    20581 ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga ggtagaagca gcgttagatg
    20641 aagccaacgt gaaacgtttt ggtcgttact tgagcgattt ccaagatgac acacaattta
    20701 tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga tgtactgtac ggagtgacga
    20761 tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat ggaagatatc aatgaagagg
    20821 cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc aaattagtag caatcgattt
    20881 ggacggtaca ttattaaatg aacaaaagct gatttcagat gagaataaac aagcattagc
    20941 acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca ggacgcccct tagctgcgat
    21001 ggcgcattat ctgcaagaat taggactggt agatgaaggt gactacagca tcactttcaa
    21061 cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg gaaaagaaag tgatggaagt
    21121 atcagatatc caacgcttat acaaactggc tcagcaattg gatctgccac tagatgtttt
    21181 gtctgacaat gtggttttac agctgcctag tgccccaagg aagcaatcct tgtacaatgt
    21241 gttgaataag ctgctgcaat tccaaccagc ggatataagc gatattacag atgaatttgt
    21301 gcttaataag gcagtaatcg cttatgacca agaagaatta gatcctaaaa tcaaagaaat
    21361 accagcagaa ttccatgaaa tgtatgagat catcaaaaca agaagtatgc ttttggaatt
    21421 tatgccaaaa ggtgttacaa aagcttatgg tatttcttta ttggcaaaag atcttggttt
    21481 gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat gatttaccga tgatccaata
    21541 tgctggacta ggcgtagcga tggagaatgc tgtgcctttc gtcaaagaag cagcagattt
    21601 cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg atccaaaaat ttgtattaga
    21661 gtagggggag tcaaatggga ttttttgata aaatcaagcg tgcttttact ggcgaagaga
    21721 aaacagaaga agttgaagta caggagaagt acgacaaggg tttagaaaag acgcgtaaaa
    21781 catttggtga acggatgaat gagttgtttg ccaattttcg aacagtcgat gaagactttt
    21841 ttgaagaatt agaagaaaca cttatcggtg cagatgtagg ttttgaaaca gcgatacgaa
    21901 tcaccgaagc attacgtcaa gaagtgaaac tcagaaatgc gaaaaaatcg gcagaagtcc
    21961 aaaatgcgat catcgagaaa cttgtggatc tctatgaagc ggaaggaatc aatgaagtaa
    22021 atgaactaaa tatccaagaa gacggactaa cagttatttt gtttgtcggt gttaatggtg
    22081 tagggaaaac aacaagtatt ggtaaacttg cccatcagtt caaaaacgaa ggtaaaaaag
    22141 tattgatggc agcagcagat accttccgcg ctggagcaat cgaccagtta gttgtctggg
    22201 gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg aggcgatcca gcagctgtcg
    22261 tattcgatgc tttggaaaga gcaaagacag agcaggcaga tgttttacta gttgatactg
    22321 caggccgact gcaaaataaa gtgaacttga tgaacgaatt agaaaaaata aaacgagtaa
    22381 tccagcggga attaccagaa gcaccccaag aaacacttct tgtattagat gcaaccactg
    22441 gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac aacagatgtg accggtcttg
    22501 ttttgacaaa attagatggt acagcaaagg gcggtatcgt acttgctatc cgtaatgaat
    22561 tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat cgatgacttg gagccgtttg
    22621 atccaaatga ttttgtcgtt ggattgttca aaggattact aaaagaagaa taacccatgt
    22681 ctaaaaagca ggagctgtga caaaagttgc agctccttgt ttgaatgatt gtttttaaca
    22741 tagctgtgtt tttgatgcct tcatttttat acttatttgt aagcggttct caaacaattt
    22801 cgttcttttt cttagacttt ttgattataa ttagattcat atgaattgga ggtcacttaa
    22861 tgatgaattt taagcaatta ttattgcatg tcaatgcgcg cccttttatc gaccaagctc
    22921 gatttggtat agaacgggaa ggacagcggg ttgatcttgc aggaaatcta gcaaaaaccg
    22981 atcatccagc aatctttggc gatcgatcct atcaccccta tatccaaaca gattttagcg
    23041 aaacacaaac agagatgatc acccctgtta ccgattctat tcccgaatta tttcagtatc
    23101 tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa agaagagatg atctggccat
    23161 taagtatgcc acctgcctta ccggaaaaag acgaagagat cattattgca aaattaaaaa
    23221 atttcgaaga tgtcttgtat cgacgttatt tagcaaaaga atacgggaag cgaaaacaga
    23281 tggtaagcgg tattcatttc aattttgaat ttggcgatga gttgctaaga acattgttca
    23341 gccatcagga agaatttcaa gatttttccg aattcaaaac agaactttat ttgaaaacag
    23401 cgagaaactt tatgcgctat cgctggatga tcacttattt attcggtgct tcaccaatga
    23461 gtgagaagaa ctacttttta gatgaatcac atccgcaaga acctgttcgc agtattcgca
    23521 acagtgcgct gggctatacg aatcatccaa atgtgaaagt ttcctatgct tctatgaaac
    23581 agtatttagc agatatcgag cgaatgatcg aagaagggaa actttcggag gagaaagaat
    23641 tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc agatttagca acaacaggtg
    23701 ttcgttatat tgaattgcgg aatatcgatt tgaatcctta tgcaagattg ggtatcaatc
    23761 cagagcaagt tcggttctta caattattcc tgatgtacat gttatggaca gaagaaaaag
    23821 aagactgtga ccaatgggta gcagaaggta cgactcgaaa taacaaagta gcactagaac
    23881 aaccatcaga tcaaacagaa tttcatcaag aaggtagaga aattcttgaa ggaatgaagc
    23941 aaatgctggt tgaattggat tggttggatt ctctttattt agtagaggaa gcattgactc
    24001 aaatggatca tcctgaacaa acgttagcag cgaaactcta tcaagaagca cagctatcga
    24061 gtcagcaaga agttgctgtg gcattgggac atcaatatta taaagaaagc catgaacgtc
    24121 cctatcagtt ggccggtttt cgcgaaatgg aactttctac tcagatattt atgttcgatg
    24181 ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc ggatcaattt ttgcgtctgc
    24241 aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat gacaagtaag gacagctata
    24301 tcgtcccact tatcatggaa aacaaaacag taacgaaaaa agtcttaaaa gaagctgggt
    24361 tccgggtacc aggaggcgca gaattttcat ccatggaaga agcggtaaaa gcttatccga
    24421 gatttgccga gcaggcattt gtgatcaaac ctaaatcaac caattacggg ttaggtatca
    24481 cgattttcaa ggaaggagct agtctggaag attatcaagc aggtttagcc attgcttttc
    24541 gtgaagacag ttcggtttta gtagaagaat ttatgccggg aacggaatat cgtttctttg
    24601 tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc tgctaatgtt attggtgaca
    24661 gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag tgaccctttg cgtggaacga
    24721 atcatcgtgc acctttagaa ttgatccaat taggtgaatt agaacaattg atgttgaaag
    24781 aacaagggtt gacgatcgaa tcagttcccc aagccaatca aattgtttat ctaagggaga
    24841 actcaaatat cagtacaggt ggtgattcga tcgatatgac tgatgaattt tctgaagctt
    24901 acaaaaaaat cgccgtttct gcggtagaag cactaggagc taagattagt ggtatcgatt
    24961 tgattatacc agataaagaa atcgatccta caactgataa aaaagcatat gggatcatcg
    25021 aagcaaattt caatccagcc atgcatatgc atgtttatcc ttttgcagga aaaggcagaa
    25081 gattgacgat gaatgtattg aaattgttat atcctgaagt attttaaaag aaaaagcttt
    25141 tgacagattg tcctatttga tgtcaatctg atcaaaagct ttttttgcat gtaaaaattt
    25201 atgtgttttt tcgttttaaa ataaacgatc ccagaagctt tttttctttt ttggtttttc
    25261 tttttcagct ggcgaagagt tttcttctgg gtattttttt tcgatatcaa tcacaggttc
    25321 atttactgct tcttttgctg ttaagagcaa tccgaattcc tcaggatttg cagtgacgta
    25381 atcgttgact acagtgaaac ggctattgtt tttcgttgct agctggatat attggttttg
    25441 gacagaaagg ggtagagccc cattcaagag tatggaagca tctggatgtt ttttgagttc
    25501 ttttaaaaag ttttctttgt tttttggttg gatcatttca gcaacagtca tactaagata
    25561 gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct tcagggttca ctaaaggtgt
    25621 accataaagt cccttatcca aatggctttt tacatcatct tgtgccataa aaaataccct
    25681 ccttcttctc tttgtctttc ttacgattat agcatattcc caataatacc taaaacatat
    25741 gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata agaatgaaaa tcattctcaa
    25801 ttagaaaggt ggattggatg cgctacggac gactagtagc attattagtt attttaagta
    25861 gtatttcatt gttcatcgga gtatcgaatg tacaattgtc agaactgctc cagttagatg
    25921 ggcaaagctt acaaatcttg ctagtgactc gtttgccacg aacgatttgt ttgatccttg
    25981 tgggagcgac cagtagtatt tgtgggttga tcatgcaaca tttgacccaa aataaatttg
    26041 tttcaccaac gactgcgggc acgatggata gtgcgcgttt aggtatttta gtggctatga
    26101 ttttcctacc tggtgcatcc ttgattgtcc gatccttgac agcattttgc tttgcttttg
    26161 caggaacatt gcttttttta tcccttacgc gattgttcct ccagaaaaat caattgatgc
    26221 ttccgttgat tggagtcatg ttagggaata tcattggttc agttgctact ttttttgctt
    26281 atcaattcca gttggtacaa aatatgactt catggcttca agggaatttt gcgactgtga
    26341 tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt gttgctcttg gtttggctat
    26401 ttgcttaccg atttacgatc attggtctgg gcgaatcgtt tgcagtaaat ctgggaatag
    26461 attaccagaa gatgcagttt ttaggcattg gcttagtatc attggctagt gcagtcatgt
    26521 tgatcatggt aggaagcatt ccttttatcg gagtgatcat tccgaatctt gtctctagaa
    26581 tatatggaga tcaagtgcac aaaacaattg gcgtaactgc tttagcaggt agtttgttct
    26641 tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc ttatgaaata ccagtcagtg
    26701 tgatcgttgg aatcatcggt ggggctatct tcctttatct tctgataagg ggacgacgat
    26761 atgcttaaaa aaaagccatc tatttttatt ggtatgcttg ttcttgtttt atttgttaca
    26821 ggaatgtact tgacgatcca tacatatgga aactggcatt ttgcatgtgc attgaggagt
    26881 aagaaaattt ttgcatttat ccttgtctcg ctagctgtca gcagtgccac aatcagcttt
    26941 cagacaatta cgcataacca gtttttgact ccttcagtat tgggacttga gtcgttatat
    27001 gtccttgtcc aaacggtcct tttcttcttt atcggaggag tgacgatgct tcagcagaca
    27061 ggtacagttt ttttcttatt gaacgttggg ctgatggtcg ggctgagtct attgttattt
    27121 ttctttcttt tgaaaaaaat gagagcgaat ctttttttat tactgatgtc agggatgatt
    27181 ttaggtacgc tttttggtag tgtcagtacc tttttacaag tcatcatgga tccgaatgaa
    27241 tatgatttgc ttcaaggcag attatttgca agctttggca atatccagag tcagtatctt
    27301 ttgcctgctt ctttgatcat tatcggctgc tggtacctgt tatttcgata tcgacatgtg
    27361 ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc tcgggataga cacagatcgt
    27421 tttgaatttt tgattttatg ttgtgtcagt cttttgacag ggacatcaac ggctcttgtg
    27481 ggtccagtta cttttttagg gtttattgtc gcaaatatca gttatcgtgt cttttctact
    27541 tatcgtcatg gtcagctatt tatcggaagc tttctggtag ctatgctgtt tctgacagga
    27601 ggacagctcg cagtagagca gattttcggc tggaatacaa cagtgagtgt cgtcgttgaa
    27661 tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg aaaggaagag tatcctatga
    27721 ttgatttaca aaaggtcaat aaactgtacc atggaaaatc agtggttaaa gatgtacagt
    27781 taacgattcc tgaaaagaag ttgactgctt ttatcggtcc aaatggtgct ggaaagtcaa
    27841 cggtgttgtc gatgatcagt cgtttgatac caaaagatac aggtgaaatc tatttagacc
    27901 ataacgaagt aaaggcttgg aaatccaatg aattagccaa aaaattatcg atcctacgac
    27961 aatcgaatga aataaatgtc caaatcaccg tgaaagaatt agtagaattt ggacgatttc
    28021 cttatacgaa aggaaaattg acgacaaaag acgaggaaat cgttcaggaa tcactggaac
    28081 acattgggtt agaagaaata gcggatcaat cgattgacac tctttcaggc ggtcaactcc
    28141 aacgtgcgta tattgcaatg gtgctagcgc aagacacgga ttatatttta ctggatgaac
    28201 ctttgaacaa tctagatatg aattttgcgg ttcaaatcat gcagatttta aaaaatttag
    28261 tacaagaaat cgggaaaaca attgttattg ttcttcatga tatcaacttt gctgcaagtt
    28321 atgcagacga aattgttgca atgaaagatg gacagttatt tacgcaagga acgacccaag
    28381 aaatgataaa aaaagatgta ttggatgaat tatacgaaat ggacatacgg atttgtgaga
    28441 ttgaaggccg cagattctgt ctttatttct aaaactaaca ttaggagaga agagatgaaa
    28501 aaattagtag taggtatctt gattctagca tcatttggtt tagcagcatg tggaaatagc
    28561 aacaacacca gtcaggcaga cacaaaaaaa agcagcacac aaactgaaac tacattgacg
    28621 ataacagata gtaatggaga ccaaatcgag gtgccaaata atccagaaaa agtcgtcgta
    28681 ttcgacaatg gttctttaga tacgatggat gctcttggtg ttggagataa ggtagttgga
    28741 gcagctacaa gcagccttcc tgagtatctt tcatcctata aaaaagtaga atcagcaggt
    28801 ggaataaaag aaccagactt agaaaaaatt aatcagcttc agccagatct aatcatcatt
    28861 tctggccgac aaagagattt tcaaaaagat ctaagtcaaa ttgctccgac cattttcctt
    28921 tctttagatg cgaaaaatcc atgggaatct ttccagcaaa atgtcactgc tttaggggag
    28981 atctttggta agcaagaaga agctaaaact caattagagg aattatctag tgcaatcgat
    29041 cagacgaaaa agaaagcaga agctactgac aaaaaagcac tagtcacact tgtgaatgaa
    29101 gggcaattat cagcctacgg atctggttct agattcggtt tcatccatga tttatttggc
    29161 tttgaacaag cagatgatca aatcgaagca tccactcacg gtcaaagtgt ttcttatgaa
    29221 tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag acagaacaaa agctattggt
    29281 ggagacgaca gcaaagatga tatttctgcc aatgaactgg tagcacaaac gaatgctggt
    29341 aaaaatcagc agatcatctc ccttgaacca gatgtctggt atttaagcgg gggcggttta
    29401 gaatcaatga aactgatgat cgaagatgtg aatcaagcat ttaaatagca taaataacag
    29461 aggattaaac tctcatacta tgtaacaata atgtttctta cattcttaaa tagcaagagc
    29521 ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg tattgatgat tttacggatg
    29581 gtttttattt atttgagaaa tcttttttca acgtgaattc attatctaca tcatacgggt
    29641 tgattctaga atggggagtg gcaaaaaata gtgtgatcca ttttttcagc atcaagatcg
    29701 cctcctttga tttgaatcaa aggtacaaag cgaaacttag ataaaactta aatttagata
    29761 tttttatttt gtaccgatct cttctttaaa cgatgttcca tctgctgctt ctcggcaata
    29821 agtccactag actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa
    29881 acaaatcgca gttgtcacaa tctcttctaa acgatgttcc atctgctgct tctcggcaat
    29941 aagcccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa
    30001 agcaaatcgc agttgtcaca atctcttcta aacaatgttc aatcagctga ccttcggtat
    30061 tagctcaaag cactgatttc acaacctcta tttaggcatg tgtctcagca agcggttgat
    30121 agttgatgat ctgcattttt cgataaaaat cggcgggaag cttactccac cgttttagta
    30181 attgatagaa ataactctct tctgctttga ttttatactc gcttaatatc aatccaaaac
    30241 tattcgtttt ttttgaagct aattttttat aaatatccaa tgctttggtt ttatcttcat
    30301 tgacaaagag tgtttttact tcaatataag aaccctcgat attttgtgtt tcttcgataa
    30361 aatagatgtt ttttatatat tccttcatag tgattcctcc aaagaagtct tttatttgat
    30421 aagtttactc taccgaagag ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa
    30481 tagttgttaa aggtttttta ttttttatag tattttttgt tatttaagaa aggaataagg
    30541 ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa
    30601 agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca
    30661 ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat
    30721 tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt
    30781 gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc
    30841 atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa
    30901 aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc
    30961 aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga
    31021 accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca
    31081 agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca
    31141 tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa
    31201 gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag accgttgcct
    31261 agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca
    31321 cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt
    31381 aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta
    31441 cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta
    31501 ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct
    31561 tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc
    31621 cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg
    31681 gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc
    31741 aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg
    31801 tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga
    31861 ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt
    31921 gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga
    31981 acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc
    32041 ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata tgctgctttt
    32101 tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac
    32161 tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatatacat
    32221 tttttattta attataatta atattgattg aagtctaggc agtcaagtcg agagtgtaaa
    32281 atattttgtg taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DAZKCC010000025        30963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000025 DAZKCC010000000
VERSION     DAZKCC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.025"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(157..321)
                     /locus_tag="ACG8VH_001236"
     CDS             complement(157..321)
                     /locus_tag="ACG8VH_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109803.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            616..1944
                     /locus_tag="ACG8VH_001237"
     CDS             616..1944
                     /locus_tag="ACG8VH_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP9109804.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2146..2979
                     /locus_tag="ACG8VH_001238"
     CDS             2146..2979
                     /locus_tag="ACG8VH_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9109805.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2995..3732
                     /locus_tag="ACG8VH_001239"
     CDS             2995..3732
                     /locus_tag="ACG8VH_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP9109806.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3887..4855)
                     /locus_tag="ACG8VH_001240"
     CDS             complement(3887..4855)
                     /locus_tag="ACG8VH_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9109807.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5087..5920
                     /locus_tag="ACG8VH_001241"
     CDS             5087..5920
                     /locus_tag="ACG8VH_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9109808.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5954..6793
                     /locus_tag="ACG8VH_001242"
     CDS             5954..6793
                     /locus_tag="ACG8VH_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9109809.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6819..7379
                     /locus_tag="ACG8VH_001243"
     CDS             6819..7379
                     /locus_tag="ACG8VH_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9109810.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7578..9299
                     /locus_tag="ACG8VH_001244"
     CDS             7578..9299
                     /locus_tag="ACG8VH_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP9109811.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9464..10606
                     /locus_tag="ACG8VH_001245"
     CDS             9464..10606
                     /locus_tag="ACG8VH_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9109812.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10622..11833
                     /gene="thiI"
                     /locus_tag="ACG8VH_001246"
     CDS             10622..11833
                     /gene="thiI"
                     /locus_tag="ACG8VH_001246"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9109813.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12406..13053
                     /locus_tag="ACG8VH_001247"
     CDS             12406..13053
                     /locus_tag="ACG8VH_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9109814.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13182..13398
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13446..16091
                     /locus_tag="ACG8VH_001248"
     CDS             13446..16091
                     /locus_tag="ACG8VH_001248"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9109815.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16401..17714
                     /locus_tag="ACG8VH_001249"
     CDS             16401..17714
                     /locus_tag="ACG8VH_001249"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP9109816.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17704..18357
                     /locus_tag="ACG8VH_001250"
     CDS             17704..18357
                     /locus_tag="ACG8VH_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9109817.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18412..19104
                     /gene="radC"
                     /locus_tag="ACG8VH_001251"
     CDS             18412..19104
                     /gene="radC"
                     /locus_tag="ACG8VH_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9109818.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19254..19454
                     /locus_tag="ACG8VH_001252"
     CDS             19254..19454
                     /locus_tag="ACG8VH_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9109819.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19708..21387)
                     /locus_tag="ACG8VH_001253"
     CDS             complement(19708..21387)
                     /locus_tag="ACG8VH_001253"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9109820.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21389..21601)
                     /locus_tag="ACG8VH_001254"
     CDS             complement(21389..21601)
                     /locus_tag="ACG8VH_001254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP9109821.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21994..23724
                     /gene="efrA"
                     /locus_tag="ACG8VH_001255"
     CDS             21994..23724
                     /gene="efrA"
                     /locus_tag="ACG8VH_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP9109822.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23721..25481
                     /gene="efrB"
                     /locus_tag="ACG8VH_001256"
     CDS             23721..25481
                     /gene="efrB"
                     /locus_tag="ACG8VH_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP9109823.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25576..25773
                     /locus_tag="ACG8VH_001257"
     CDS             25576..25773
                     /locus_tag="ACG8VH_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109824.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25926..26411
                     /locus_tag="ACG8VH_001258"
     CDS             25926..26411
                     /locus_tag="ACG8VH_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9109825.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26515..27108)
                     /gene="clpP"
                     /locus_tag="ACG8VH_001259"
     CDS             complement(26515..27108)
                     /gene="clpP"
                     /locus_tag="ACG8VH_001259"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9109826.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27288..27815
                     /locus_tag="ACG8VH_001260"
     CDS             27288..27815
                     /locus_tag="ACG8VH_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109827.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27906..28643
                     /locus_tag="ACG8VH_001261"
     CDS             27906..28643
                     /locus_tag="ACG8VH_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9109828.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28647..29564
                     /gene="miaA"
                     /locus_tag="ACG8VH_001262"
     CDS             28647..29564
                     /gene="miaA"
                     /locus_tag="ACG8VH_001262"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9109829.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29566..30795
                     /gene="hflX"
                     /locus_tag="ACG8VH_001263"
     CDS             29566..30795
                     /gene="hflX"
                     /locus_tag="ACG8VH_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP9109830.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(30829..>30963)
                     /locus_tag="ACG8VH_001264"
     CDS             complement(30829..>30963)
                     /locus_tag="ACG8VH_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9109831.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT        10222 a         5291 c         6604 g         8846 t
ORIGIN      
        1 tcgagtccta ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg
       61 ggtttctttt tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg
      121 taatctttac gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct
      181 aaatacgaaa taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg
      241 gatgacattc ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc
      301 gccagcaatc aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct
      361 ttcaatgagt attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat
      421 tatacaggcc attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat
      481 accgtatgac atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca
      541 tcatagaaga gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt
      601 tggaaggatg attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag
      661 tttcaagcaa atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact
      721 gcttctgctg aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg
      781 gctacgggga aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg
      841 aacacattcc gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa
      901 aattacactt ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt
      961 gcaacaggaa aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa
     1021 caagatggcg gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct
     1081 aaatcggtga tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg
     1141 aacaaattac tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca
     1201 ttagacgcta ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct
     1261 atctcattag gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac
     1321 tatcatcttg accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa
     1381 ttagatgatt atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct
     1441 tatacagtag agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt
     1501 gatatggaaa ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg
     1561 tttggttgtg atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct
     1621 tattcaatgg atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat
     1681 tttggagaaa gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg
     1741 caatcaacaa aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc
     1801 tttgttatga gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat
     1861 ttattgacga aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg
     1921 gatccaatgg gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa
     1981 aaagagctgg cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt
     2041 ccctgtgcta taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa
     2101 atatgatgac gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact
     2161 gttttatctg atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat
     2221 cagacctatc gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta
     2281 aaaaaaggat tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt
     2341 gtaccggata cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg
     2401 atcgagccgg gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag
     2461 caaacagcgc cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa
     2521 aattcttttg aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt
     2581 tctttggcag aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt
     2641 aaagaacgtg tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga
     2701 gacctgtgga gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca
     2761 gctgtttctt atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc
     2821 cgaccggaat ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac
     2881 cgactgccga tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt
     2941 tacggttccc aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca
     3001 aaaaaatata ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg
     3061 acatttcctt cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg
     3121 ggaagaggca gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc
     3181 atggagatta atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg
     3241 gagtacaata agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa
     3301 ttagtcaaaa tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt
     3361 gttaatgaag agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag
     3421 gaaaaaattg aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat
     3481 atcgatagta atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta
     3541 ggatatgact ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa
     3601 gtagagtacg ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc
     3661 aaaacaaaac atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg
     3721 aaaaataaat aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat
     3781 ttacgaaaat tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt
     3841 tttgtcacag cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa
     3901 acgaaatcaa ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg
     3961 ctttttgact attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc
     4021 cgccttcata atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta
     4081 cagggatttt ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc
     4141 ccgcacctaa agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata
     4201 agtccccttg catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa
     4261 tgtccagtcg ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta
     4321 agccgatggg acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg
     4381 tcacgtagcg tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg
     4441 cttcttcaca acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa
     4501 gttcattact tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg
     4561 tatcaagaaa aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg
     4621 atatacctga tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca
     4681 tgatgtgtgg tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga
     4741 ttgtgatttc tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca
     4801 tgttttcttt catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac
     4861 agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga
     4921 gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat
     4981 taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct
     5041 tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg
     5101 attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga
     5161 atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa
     5221 gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt
     5281 ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat
     5341 gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt
     5401 tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa
     5461 acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta
     5521 cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc
     5581 catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa
     5641 tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga
     5701 taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact
     5761 agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa
     5821 taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa
     5881 gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa
     5941 aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa
     6001 tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat
     6061 tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt
     6121 ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat
     6181 tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa
     6241 ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt
     6301 aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg
     6361 tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac
     6421 gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca
     6481 gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt
     6541 agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt
     6601 cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct
     6661 gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc
     6721 agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg
     6781 tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc
     6841 aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata cccacggctt
     6901 ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg
     6961 ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag
     7021 cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg
     7081 ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct ttttcattac
     7141 taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat
     7201 ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact
     7261 ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag
     7321 aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat
     7381 cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag
     7441 ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga
     7501 cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga
     7561 aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta
     7621 tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat
     7681 tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg
     7741 atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc
     7801 aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag
     7861 tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag
     7921 aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat
     7981 tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag
     8041 aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc
     8101 aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg
     8161 gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg
     8221 aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac
     8281 aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag
     8341 aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca
     8401 atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga
     8461 ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta
     8521 accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga
     8581 ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac
     8641 gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga
     8701 tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag
     8761 tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa
     8821 aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga
     8881 aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt
     8941 ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca
     9001 tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga
     9061 agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc
     9121 gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca
     9181 ataaagaata tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag
     9241 aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa
     9301 gcaaaaaagt tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt
     9361 ttgtatattt gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac
     9421 aaccgttata atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa
     9481 cagtgcaaca accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg
     9541 gattattggg aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca
     9601 gcaagcaaga aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac
     9661 ttcaggcgga acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga
     9721 atatgggaat catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga
     9781 acaattaaaa gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt
     9841 acgtgtcgac gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc
     9901 tgtgaataat gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga
     9961 ttttcctaaa attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc
    10021 gcaatggttg acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc
    10081 acggggaact ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg
    10141 tggagggcaa gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat
    10201 ggttaaagct ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg
    10261 cttaaaagta gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga
    10321 aggaactgac tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt
    10381 tctcgtccat gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc
    10441 acgtaaacat atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac
    10501 atctgctgtt cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat
    10561 gattattttc aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct
    10621 tgtgaaatac agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg
    10681 aaaaagcttt atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga
    10741 aatcaaaatc catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca
    10801 gctagttatt ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg
    10861 tgtagaaaaa gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta
    10921 tactgggaaa gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac
    10981 aagtaatgaa ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca
    11041 agttcaaatg cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta
    11101 tctatcgtat gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg
    11161 aatgcttatg ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg
    11221 gggagtggaa attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact
    11281 gcaaaaagca aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat
    11341 cgaagtgcca tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg
    11401 gatgacaatt actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca
    11461 tggtttagtc attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag
    11521 tatggtggct atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga
    11581 taaacttgaa atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc
    11641 ttttgaagat tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga
    11701 aaaggtccat caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc
    11761 tggcttgaag atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc
    11821 agaattgcta taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac
    11881 gaaaaaatag ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga
    11941 ataccattta tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa
    12001 ttttatttag agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa
    12061 atagcgaggt tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt
    12121 gcttttcaaa tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc
    12181 gtgtatcaga ggttgcgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    12241 atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca
    12301 ccgttttatt tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa
    12361 acatgttaca ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca
    12421 gatacactgc caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg
    12481 ctggatgact caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt
    12541 ccttctgcaa cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat
    12601 ggctatgatg tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa
    12661 aaacgaatcg cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt
    12721 agaagaaatg aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat
    12781 cgagtaattg atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca
    12841 aaaaatgtga cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat
    12901 gaaattgtca aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc
    12961 ccagcttatg tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac
    13021 tttgatgaga attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt
    13081 gcgagaactc gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca
    13141 cgaccacttg ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga
    13201 ggagtaactg gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac
    13261 aaccagtgaa ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg
    13321 atcgttaact cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt
    13381 aaatcatacg tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa
    13441 acagcatgac ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg
    13501 gacgttatca gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa
    13561 aaccttattc aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg
    13621 cttgggatac gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata
    13681 ctttatggct gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga
    13741 agcttagaga gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag
    13801 tgtgggaatg gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct
    13861 tatctttaga ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac
    13921 gtaaagtttt tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca
    13981 actgggatcc tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg
    14041 aaggcgcctt ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg
    14101 cgacaacccg tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg
    14161 agcgttatca agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc
    14221 caatcattgc agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc
    14281 cagcacatga tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg
    14341 ttatggatga tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt
    14401 ttgctgctcg taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg
    14461 atacgatgat ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc
    14521 tgtctactca atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag
    14581 aaacagatca agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga
    14641 tggaaaatgt ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg
    14701 catggtatca caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg
    14761 aaaactgggt acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt
    14821 tctcaacaat gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta
    14881 gcactttagt aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa
    14941 gcttggaatt tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg
    15001 acgaacaagg gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa
    15061 tcgaaaagta tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac
    15121 aagatgtccg cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct
    15181 ggaatgcttc acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt
    15241 tatctggaga aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg
    15301 aacgagtaac tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata
    15361 actttatctg ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg
    15421 gagatgatgc tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga
    15481 tcctacgttt attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc
    15541 ctcatcaggg aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg
    15601 atgaaacagc ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata
    15661 ttcgttcaga agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg
    15721 atgctaaaat cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc
    15781 ccgaagagtt gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc
    15841 tgacaggtgc agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca
    15901 aacgattaga aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac
    15961 tttctaatga acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa
    16021 aagaaaaaga ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta
    16081 ctattcaata agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc
    16141 tgattgaaca tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga
    16201 gatgtgacat aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg
    16261 agggaggtgg atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct
    16321 gtttcttttc tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa
    16381 gagaaatggg ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat
    16441 taccatttgg ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc
    16501 atccagagag aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg
    16561 tagcttattt gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac
    16621 cttatatcga aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg
    16681 atttgatcca gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag
    16741 cagtagctgg tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga
    16801 tgaagaaagt ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca
    16861 atgtagctgt accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac
    16921 ttggagatac aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc
    16981 cagtagtaac aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag
    17041 caaaaaatgc accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg
    17101 atgagatgtg gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa
    17161 ccactccgct tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt
    17221 ttgtttactg tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga
    17281 agaaatctcg atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag
    17341 atggtgccca taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca
    17401 aaggatatca aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc
    17461 tagaacgatt gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa
    17521 aagccatcga attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat
    17581 tgtggcaatt cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg
    17641 tgaccggttc actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag
    17701 atcatgaagt ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta
    17761 gtgtattacg aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac
    17821 ctgtatttaa aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt
    17881 ttctcggaat ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg
    17941 cttgaacgtt tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct
    18001 tttttagaag aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt
    18061 gaacttttat tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa
    18121 aatgttcaac gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt
    18181 acagcgaaga aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct
    18241 gcggctgaga tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc
    18301 aaaaaaacag ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa
    18361 tccatctctt ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa
    18421 cgattgatca aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc
    18481 gcggaagctt tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct
    18541 tatagtgtga tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta
    18601 cgacaggcta ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc
    18661 gaaatcaaag ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct
    18721 ccaccggttc gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac
    18781 aaacaagaac atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa
    18841 acagtgttca tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat
    18901 gctgtacgtt attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt
    18961 attccatcgc agcaggatat gaattttacc aaaaggatac aaaagtgtgg agaaatgatg
    19021 gggatcactg tactggacca cttgatcatc ggacgcaaac gatattttag tttgagagaa
    19081 gaaggaatga tggaagagaa gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca
    19141 attatattgt aattttgttt gggaatagaa gttgaaacat ctgttgcacc tctatcgtac
    19201 acgagcaatg ttacaattta aattagtgct tttggcacga ggaggatatt attatggaaa
    19261 acggaacagt aaaatggttc aacgctgaaa aaggatttgg atttatctca cgtgaagatg
    19321 gaagcgacgt attcgttcac ttctcagcta tccaaggtga cggattcaaa acattagaag
    19381 aaggtcaagc agtgactttc gacgttgaag aatcagaccg cggcccacaa gctgcaaacg
    19441 ttgtaaaagc ttaattttat ctgtaaatca aattgatggt agacatcaat ttttacgaga
    19501 gaacaaagcg agtaacaaaa atacctgcca caagagacaa tttctcttgt ggcaggtatt
    19561 tttttgtatg aaaaagtaaa taaatttaag aggctatgaa agagccacct tgtatcaagg
    19621 aataagaaca aacaaaaatg gcttctcatg tttttcattt gttttctctt atattcagga
    19681 caaggtgctg atttcataac ctcttattta ttcttcatct ggtgtcatta ccattggcaa
    19741 aatcattgga tgtctttctg tacgttcgaa taagaaaggc tgaagcgcgc cagtcatcgc
    19801 ttctttcaat ttatattctg aacagtcagg tccattcaaa cattctctta aagcgtggaa
    19861 taagattcgc tgagcttcat tgatcatttc tcctgattct cgcatgtaga caaatccacg
    19921 agagagaata tctggaccag ccaagatttc ttttttcttg atatccactg tcgcgacagc
    19981 taatacaaga ccttcttctg aaagcaggtg acggtcacgt aaaacgacat taccaatatc
    20041 accgatcccg ctaccatcga tatacacatc tccagccaca aaatgcccag caggacgcgc
    20101 agaatcgcta gttaacgcta aaacatcgcc attttccatg atgaagcagt tttctgcagg
    20161 aacacctaca tcttgtgcta gagaagcatg tactttcaac atgcggaatt ctccgtggac
    20221 aggaataaaa tattttggct tcatcaaacg aagcattaat ttttgttctt cttgtccacc
    20281 gtgaccggaa gtatggatgt tattgatttt tccgtgaatc acttctgcac cagcttcaga
    20341 cagcaaattg atcaaatgat tgacacttgt tgtattacca ggaataggtg aacttgaaaa
    20401 gatcactgta tcgtctggct ggatttggat ttgacggtga gtaccattcg cgatgcggct
    20461 caacgcggcc atcgcttctc cttgagaacc tgtacatagg atcatcgttt cattggcagg
    20521 caagcgattg atttcgtgtg catctacaaa tgtgccgtct ggaactttga tatatcctaa
    20581 acgttgaccg ttgacgatgg cattttccat gcttcgtccg aaaacagcga ttttacgacc
    20641 agttgccaca gcggcatctg ctgcttgctg caaacggaag atgttagaag caaagctggc
    20701 aaaaatgata cgtccttcga tttttgaaaa gatcttcaaa atagaatgcc caatcgtttt
    20761 ttctgattta gtaaatgttg gaatctcagc atttgtacta tcagataaaa ggcacaagac
    20821 tccttcttcc ccgatacgag ccattttatg caagtttgct ggttcgccaa caggagtgaa
    20881 atcgaatttg aagtctccag tcgctacgat atttccagaa ggtgttttca ccacgatccc
    20941 taatgcatct gggatactat gggtcgttct gaaaaagctg acagaagtct ttctaaaacg
    21001 gatgatcgta tcttcattga tctcatggag ctctgcatct cgaagcaagc catgctcatc
    21061 taatttgttt gtgatcaacg ctaatgcgag aggaccagca tagataggaa tatttgcttg
    21121 tttcagcaag tagggtattc cgccgatgtg gtcttcgtgc ccgtgagtaa tcaccaaggc
    21181 ttttactttg tgtaagtttt gtatgatgta gctgtagtcg ggaatgacat agtcaattcc
    21241 aagtaaatcg tcttccggga atttgatccc ggcatcaatc aaaatgattt catcttggaa
    21301 ttgtacccca tacgtatttt tcccgatttc acctaatcca ccaatagcaa agacgcctgt
    21361 ctcgttattt ttgatggaaa gtttcatatt aaaactccac taatgagaaa tcagcatttt
    21421 ctttttcgta ctctagatga cttccttcta gaagttgaac gaattcaata ttgtatgggg
    21481 tattttcttc cacacgttgg cgagcgtcta catcgctttt tgcctcaatg taaagagatt
    21541 ttgtttcttc ccgtttaggg ttgcgtactt ttgtttcttg ataataaact ttatagatca
    21601 ttgttttgtc tcctttatct gcgtaaagca gtgatttagc tagttgattt ttttagacaa
    21661 cacaaaacga ggggacaagc cacctggttt ttcatatgct ttgactagaa tactggattc
    21721 gagtcaaaga gacaagcctc gtttttgctt atctgttcgg atattttttc aaccgtacct
    21781 tctgtataaa aaatatacaa caattaattg ttattctagc ataaaacagc aaataagtat
    21841 agaaatcaac tagattgaaa gaaaaaatca atgtttatca tcccgctata atcataatga
    21901 cttttttatg agagaagtgt tctattaata ggctcgttgt gctatcattt ttagtaatga
    21961 aaatgtaaca aaagattaaa aggagatgag aaagtgaaac taatgtggca ttataccatg
    22021 cgttataaga aattcttgtt tttcaatttc atatgcgtat ttggttttat tttgattgaa
    22081 ttaggacttc ctacgatttt agcacgtatg atcgatgtag gaatccgcaa tgatgatttt
    22141 gattatgtga agcaacaagg gatatttatg atcgttatta ctgtaatcgg tattattttg
    22201 aatatcttgt taggttattt tggttcgagg attacgacta atatcgtagc agatattcga
    22261 gatgatttgt tcgaaaagat ccaaagttat tcgcatcagg aatacgaaaa tctaggcgta
    22321 tcttcattga tcactcgtac aacaaacgat gcttatcaga tcatgttgtt cctgcaaaat
    22381 attttacgta tcggttttat ggcaccaatg atgttcattg tcagtttgta catggtcatg
    22441 cgtacaagtg tgacactcag tttgtatgta gtaggtgcat tacctttact attattggca
    22501 gtcgtagcga ttgctaaggt gtctgaaccg ttatctaaaa agcaacaaaa aaacttagat
    22561 aaaatcaaca gtatcttgcg ggaaaatctt tcaggattgc gtgtgattcg tgcattcgtc
    22621 aatgaaaagt ttgaagaaaa acgttttgct gtagtcaatg agaattatgc ttctagttca
    22681 aaaagcttat tccgtttgat ggctgttgca caaccgggtt tttatttctt atttaatatc
    22741 gtaatggtat tgatcatctg gagcggggca gtgcaaatcg atgcaggcga gcttcgtgta
    22801 ggagatttga ttgcgtttat cgagtatata ttccatgcct tgttctcatt catgttattt
    22861 gctagtgtgt tcatgatgta tccaagagca gctgtttcag ctggacgtat ccaagaagcc
    22921 ctagatgcca aaccttcgat tgtagaagat ccaaatggta tatctgatac aaaaactaaa
    22981 gggtatatcg aatttcaaaa tgtaacattt gcttatccag gccattcgca agagccagtg
    23041 atacgaaatg tcagttttac agcctctcca ggagagaccg ttgcttttat tggaagtacc
    23101 ggtagcggaa aatctacgtt gatccaattg atccctcgct tttatgatgt gagtgaagga
    23161 aagatcttga tcgatggagt agacgtgaga gattatcggc taagcaagtt gcgagataag
    23221 attgggttta ttccgcaaaa agcgctgcta tttactggaa cgattgcaga taatttgcga
    23281 tacgggaaag aagatgctac gcaagaagaa atggaacgtg cagctgagat tgctcaagct
    23341 tcagaattca tttctcgaaa accagatgga tttaatgaac atttatcaga aggtggttcc
    23401 aatttttcag gtggacaaaa acaacgattg gcaatagcca gagccatcat acgccgtccg
    23461 gaaatctata tttttgatga cagcttttct gcattagatt atcaaacgga tgcgaagtta
    23521 cgtgctcgtc tgaaaaaaga aacgacagaa tctactgtat tgatcgtagc acaacgtgtg
    23581 ggaacgatca tgcatgcaga taagatcatc gtattaaatg aaggagaagt cgtcggtatg
    23641 gggacgcatc gggagttgtt ggaaaaatgc ccgatttatt atgatattgc cgcttctcaa
    23701 ttatcaaagg aggaattagc atgaaaaaaa ccttttcttc cttaaaacgt ttagctcgat
    23761 atatcagacc gtatcgtgag acttttcttt tagtgatcct atttacagtt ctgacagtgg
    23821 ctttcaacgt ggctttgcca tatgttacag gacttcctac tacagaaatc agccgtaata
    23881 ttgccaatgg cgaatcaatc aatttttctt atatattcaa atgtctcatt tggatcacag
    23941 ttgttggaat agggtattgt atttcgcaat tactttctgg tgttttgatg acaaatgtcg
    24001 ttcaaagtgc catgcatgat ttgagaagag acatagaaga aaaaatcaat cgtcttccgg
    24061 tttcatattt cgataaaaac cagcaaggaa atattttgtc tcgtgtgacg aatgatgtag
    24121 atgcagtcag cggtgcttta caacaagctt ttattggtat cgtaaatgcg atattgggaa
    24181 ttacgttggc tgcagcaatg atgttttata tccagccgat gatggcgctg atctctatga
    24241 tcatgattcc aacatcgatc tggatctcaa aacgagtgat caatgcttcg cagaaatatt
    24301 ttcaaagcat gcagaattct cttggtgagc taaacggcta cgtccaagaa aatatgacag
    24361 gttttagtgt attgaaagta tatggacgcg aaaaagaaac attggaagga ttcaaaaaag
    24421 ttaatcacag cttgaaatac tttgggtttc gtgcagcatt catttctggc ttgatgatgc
    24481 ctctagttca attaactgcc tatgctactt atattggtat ggctgttcta ggaagctttt
    24541 atgttattac tggggcgatc gttgtcggtc aactacaagc attcatccaa tatatttggc
    24601 aaatcagtca gccaatgggg aacgtgacac aattgtcttc agttttgcaa agtgcatcag
    24661 cggcaacgaa acgtgtattc gagattttag atgagccaga agaaaaagaa aacaaagtag
    24721 atgcttcact gccagaaacg attttaggag atgtgacatt cgatcatgtc gattttgcat
    24781 atgatccgaa gaaaccatta atccaagatt tgaattttga agtgaaagct ggtcaaaccg
    24841 tagctgtggt cggtccgacc ggtgccggca agacgacatt gatcaatcta ttgatgcgtt
    24901 tttatgacgt agacaaagga gcaataagga ttgatggaat cgacaccaaa tcaatgagtc
    24961 gcagtgatgt gcgttcgcta tttggtatgg tcttgcaaga tgcatggctt tatcaaggaa
    25021 cgattgcaga taatatccgc ttcggaaagc ttgatgcgac agattatgaa gtagttgatg
    25081 ctgcgaaaac agcaaacgta gatcatttta tccgaacgat gccagatgga tatgatatgg
    25141 ccatcaattc tgagggagac aatgtgtctt taggacaaaa acagttgctg acgattgccc
    25201 gagcagtggt ctccgatcct aaaatcttga ttttagatga agcaacaagt tcagtagaca
    25261 cccgtttaga agcgttgatt caaaaagcaa tggatcgcgt gatggaggga cggacaagtt
    25321 tcgtcatcgc tcaccgttta tcgacaatcc gtgatgcaga tctgattcta gtgatggatc
    25381 aaggtcagat cattgaaaaa ggaacgcatg agagtttatt ggcacaaggt ggattttatg
    25441 aaaaactgta taacagccaa tttgctgagg aagcagaata agtcatataa gaataaaaca
    25501 gtttaaaact tattttttgt tctctattct tgataatggt aaagtaaagt tatcaagaaa
    25561 agtgaggaca cggagatgga acagttttta aatgaaaaaa ttactgaagc caaaagagaa
    25621 gttgatcgtt taacgcacgt ctgtcagacg aagccttttg attcagctga tttttttgaa
    25681 aatgagtttc aaatcgaacg cttggcagca caaatagaag tgtacgaatt aattttaaaa
    25741 aaagtaatag aagaaaatat agatgtacaa taaaaacagg taaaacgtca gttaccagat
    25801 actgtaaata gacatctggc ctgagcaggt tttacctgtt ttttttctta tgaaataaat
    25861 atatgctatg atacctttat tcaataggat tagtttctac aaaaaatgaa aggagaaaaa
    25921 taaatatgga acggtttata gcatcaaatg ccacagtgat tggtgatgtg acattgtcag
    25981 aagatgtgac gatctggtat caagctgttt tacgcgggga cagtaattgg ataaaaatag
    26041 gacaaagaac gaatatccaa gatggtacaa tcatccatgt cgatcatgat gcacctgtag
    26101 atattgcgga gaatgtgacg gtaggacatc agtgcatgct acatggctgt acaatcgaaa
    26161 aaggggcgct gatcgggatg gggaccacga tattgaatca tgcggttatt ggtgagaata
    26221 gtttgattgg tgctggatca ttagtaacag aaggaaaagt tattcctcct aatgtcttgg
    26281 cttttggtcg cccagctaaa gtgatccgtc ctttgacaaa agaagaaata caaaaaaaca
    26341 aagaaaacat ccagcattat gtcgagatag gtcggatgca tgcgcaagga aaattcaagg
    26401 agcaaagata ggcagtagca tccataaaat aaaaaaggat ggtcagtttg agttgaccat
    26461 ccttttttat ttggtttaga taagagagct taaaaaagct tcctttgaaa cagattaatt
    26521 taatgaagaa ctgttttcca tgacttcatc gatcaatcca tatgcttttg cttgttcagc
    26581 tgtcatatag ttgtcacgat ctgtatcttt ttcaatcact tcaagcggtt gtccagtgcg
    26641 ttcagctaaa attttgttta agcgttcgcg tgtttgcaaa atatggcgtg ccgcaatttc
    26701 gatttcagtt gcttgccctt gagcgccgcc aagaggttga tggatcatga tctcagcatt
    26761 tggtaaagca aatcgtttac cttttgtacc agctgtcagc aagaagcttc ccattgaagc
    26821 agccataccc ataacaattg tttggacatc tgctttgaca aagttcattg tgtcgtaaat
    26881 agccatacct gctgtaactg aaccaccagg agaattgata taaagataga tatctttttc
    26941 agaatcttga gcgtctaaga aaagcaattg ggcgatgatc gaattagcaa gatcatctgt
    27001 tacttggccg cttaacatga tgatacggtc tttcaataaa cgtgagtaga tatcgtaagc
    27061 tctttcacct cgtgaagatt gttcaatgac tgtaggaatt aaattcataa tagagttcct
    27121 ccttgatttc gtttagaaat agtgtaccat aatttagttt ctaataccta aaatgtagta
    27181 tacatttttg gtcaaaaaag gtcaaataaa aaacttaggc aaattagtcg cataaacccg
    27241 ctttatggta tgataagcag agaaacaaaa caaggaggaa caacagattg atagttggta
    27301 ttactggcct tatcggagtg ctgacaggtg ctgtagttgt ctatatcggc atgatactgc
    27361 aaatgcgtca cttggaaaat aaagaacagc gtagacgtga attggaattg aaagtcaaag
    27421 aaatcgagac gctgaatcaa ttaaataaaa agatcaacga gatcttgcaa aaacggacaa
    27481 cgctgatgga agaatatgtc agctttgatg catttgatga ttgttatatc acgattgacg
    27541 attttgcgta tctccaatca ttttcagcgc aaaatagttt ttatctgcca aattatattt
    27601 tagacgagct atttaagaat atctcccatc gaaaagtgat tttatctcca gatgaaacaa
    27661 tgaagattgg tggatatgct tataaaggcg gacgcgtgat tttagagaat tttacggata
    27721 gtctgactga aatgatcaat gaaaaaaaag ctcagttgaa gactatgacg cgccagccga
    27781 ttggtttctt ctcgaaagat gatgtttgga gctgatattt tgaagcattg ccgttgcaca
    27841 tgagagcgtg gtaatgcttt ctttttttct taatgataca atagaactga acggagtgaa
    27901 gaaagatgtt acaaatctat gcccatcgcg gaagcagcgg cacccaccca gaaaatacgc
    27961 ttcctgcatt tgcagaagct gtacgagtag gtgcagatgg tatcgaacta gatgtccatc
    28021 taagcaaaga tggttatttg atcgttatgc acgacgaaga agtggaccgg acgactaacg
    28081 gtaaagggct gattcgagaa aaaacattgg aagaactcaa aaaattgaat gcagggggca
    28141 aattcagcaa agaatttcca gcagctaaag ttcctgaatt gagagaagtg ttaggactgc
    28201 tcctccaaaa aaattaccga gggatgctaa tgattgaaat taaaactgat cagtatgaat
    28261 atcccggtat cgaagaaaaa ttagccgaaa cattaaaaag tagagattgg ccttttcaac
    28321 atgcatattg cagctttcat ttggattctt tggaaaaaat cgcattactg gaaccggaga
    28381 tacagctgga cttattgatg agtacttctg ataaaaagat ccaactagca gcagaaaccc
    28441 cttatataga aggagtacat ccaaaaaata cttggttgtt cgaacatcca gtccaggcag
    28501 ctgattttcc aaaagcaatt cgtgcatgga cgattaacga tgaagaagaa atggccaaat
    28561 gtcaagaact caaaatagat ggaataataa ctgattttcc ggagaaagct cttcggtgga
    28621 aacaatcaac gaaaagtagg taaaatatgg aaaaagtatt agtgatcgtc ggtccgacag
    28681 cagtcggtaa aacagcgcta agtgtggaac tcgcacaact atttggagga gagatcatca
    28741 gtggtgattc attgcaaatc tataaaaatc tggatatcgg tacagcaaaa atcaaagaag
    28801 aagagatgaa taatgttccg catcatttga ttgatgtgat cgaacctact caacgttact
    28861 cagccgttga ttttcagcat tcaggtcgac aattgattac cgagattgcg aaccgagggc
    28921 acctacctat catagctggt ggtacgggtc tctatatcca atcccttcta tatgattatc
    28981 agttaggagc gacagaagaa gcgaatcctg agattagaaa aaagtatgaa acgttagctg
    29041 aaacaattgg ttcccaagca ttatggaatc agttgaaaga aaaagacccg gcagctgctg
    29101 agaaaatcca ctggaacaat caacgcaaag tgataagagc tctggaagtt ttagagacga
    29161 caggacatag tatcacggca cctaaagaag agccaaagca attgtatgat tacctcatga
    29221 ttggattaga tacgaagcgt agtattcttt atgaacgaat caatcaaaga gtcgatttga
    29281 tgctagcaga aggtttagta gcagaagcgc gtaaggtgta tgagcttgga gaagtccagg
    29341 ccagtcaagg tattggatat aaagaatttt tcccttattt tgaagggaaa gaaacattgg
    29401 aagaagcaac agaacaagtc aaactacatt cgagaagata cgcgaaacgt cagttgacgt
    29461 ggtttcgtaa ccgtttggat gcacagtggt ttgatcttat aagtgagcca aatcaaaaaa
    29521 gtcagattga gcaaacaatc aaaaaatggc tggaggcgga ataaaatgga agaaaaagtg
    29581 atcatcgtag gggtagaaac cgaagcaaac cagcgttact tttcagagtc aatggaagag
    29641 ctagttcagc tgacgaatac cgcatcagga gaagtcgtat tcacaattac acaaaagaga
    29701 ccacaagtgg atcggcaaac gattatcggt aaagggaaat tacaagaatt agttcaacaa
    29761 gcggatgcgc atgaagcgga tttgatcatc tttaatcatg agctaactcc gagacagagc
    29821 caactgatta ccgatgctgt tggtcttccg gtaattgatc gcgtgcagct tatcttagat
    29881 atttttgcga tgcgtgctcg ttcgaaagaa gggaagctgc aggttgaact tgctcagtta
    29941 gaatacctgc tgcctcgttt agtagggcaa gggaaaacat tatctcgtct tggcggtggg
    30001 atcggaacaa gagggccagg tgaaacaaag ttagaaacag accgccgaca tatacggaat
    30061 aaaatttttg ctgttaagaa agaattgaaa gaagtagaag ctcatcgaga gcgcaatcgc
    30121 caaaaaagaa aaaatagcga gatttttcag attggactta ttggctacac gaatgccggg
    30181 aagtcaacta tcttgaatct attgacacaa gcggatactt attcgaaaga tcaattgttt
    30241 gcaacgttag atccattgac taagagatgg cgttttgctg aaggatttga gattactgta
    30301 acggataccg tcgggttcat ccaagacctg cctacgcaac tgatcgatgc tttccattct
    30361 acattagaag aaagtcaaaa tatggatctt ttgctacatg tagtcgatgc tagttctcca
    30421 gatcgtattt tacaggagca gactgtctta aaattgatgg atgaactgaa tatgaaagaa
    30481 atgccgatac tgactgttta caataaggca gaccagatcg acccagcgat gttcacgcct
    30541 acgctattcc caaatgtatt gatttctact cagacgaaag aaggtaagat ggcgttgatc
    30601 gaagcaatca aacgtcagct gatggaacta atgacccctt atacactttc tgtgccaagc
    30661 aacgaaggac agaaattaag cgagttgaga agacaaacaa tggttttgga cgaagcgtac
    30721 atggaagaaa caaacgaata tgaagttaaa gggtttgcaa aaaccaattc aaaatggtta
    30781 agaaaaaacc aataaaattt tattttttta acaatattat ttgtcaaatt aagcgagaca
    30841 aaactttcca tttacgtaac tcaaaaaaac ttctaatggt gtttggtaat ttagtgattt
    30901 tctagggata tggtttcttt tggacgcaac ggatgagatg aatccttgat tgacttggtt
    30961 gaa
//
LOCUS       DAZKCC010000026        30381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000026 DAZKCC010000000
VERSION     DAZKCC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.026"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..1682)
                     /locus_tag="ACG8VH_001265"
     CDS             complement(372..1682)
                     /locus_tag="ACG8VH_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP9109832.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1694..3052)
                     /locus_tag="ACG8VH_001266"
     CDS             complement(1694..3052)
                     /locus_tag="ACG8VH_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9109833.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3254..4822)
                     /locus_tag="ACG8VH_001267"
     CDS             complement(3254..4822)
                     /locus_tag="ACG8VH_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP9109834.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4826..5236)
                     /locus_tag="ACG8VH_001268"
     CDS             complement(4826..5236)
                     /locus_tag="ACG8VH_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9109835.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5233..6156)
                     /locus_tag="ACG8VH_001269"
     CDS             complement(5233..6156)
                     /locus_tag="ACG8VH_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP9109836.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6153..7031)
                     /locus_tag="ACG8VH_001270"
     CDS             complement(6153..7031)
                     /locus_tag="ACG8VH_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP9109837.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7018..8355)
                     /locus_tag="ACG8VH_001271"
     CDS             complement(7018..8355)
                     /locus_tag="ACG8VH_001271"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP9109838.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8358..9461)
                     /locus_tag="ACG8VH_001272"
     CDS             complement(8358..9461)
                     /locus_tag="ACG8VH_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9109839.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9535..11043)
                     /locus_tag="ACG8VH_001273"
     CDS             complement(9535..11043)
                     /locus_tag="ACG8VH_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9109840.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11009..11701)
                     /locus_tag="ACG8VH_001274"
     CDS             complement(11009..11701)
                     /locus_tag="ACG8VH_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9109841.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11723..12697)
                     /locus_tag="ACG8VH_001275"
     CDS             complement(11723..12697)
                     /locus_tag="ACG8VH_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109842.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12997..13869
                     /locus_tag="ACG8VH_001276"
     CDS             12997..13869
                     /locus_tag="ACG8VH_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109843.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14327..16525)
                     /locus_tag="ACG8VH_001277"
     CDS             complement(14327..16525)
                     /locus_tag="ACG8VH_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109844.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16757..17749
                     /locus_tag="ACG8VH_001278"
     CDS             16757..17749
                     /locus_tag="ACG8VH_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9109845.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17809..19230
                     /locus_tag="ACG8VH_001279"
     CDS             17809..19230
                     /locus_tag="ACG8VH_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9109846.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19223..20722
                     /locus_tag="ACG8VH_001280"
     CDS             19223..20722
                     /locus_tag="ACG8VH_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP9109847.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20761..22155
                     /gene="uxaC"
                     /locus_tag="ACG8VH_001281"
     CDS             20761..22155
                     /gene="uxaC"
                     /locus_tag="ACG8VH_001281"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9109848.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22314..22997
                     /locus_tag="ACG8VH_001282"
     CDS             22314..22997
                     /locus_tag="ACG8VH_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP9109849.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22999..23835
                     /locus_tag="ACG8VH_001283"
     CDS             22999..23835
                     /locus_tag="ACG8VH_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9109850.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23846..25600
                     /locus_tag="ACG8VH_001284"
     CDS             23846..25600
                     /locus_tag="ACG8VH_001284"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109851.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25876..26217)
                     /locus_tag="ACG8VH_001285"
     CDS             complement(25876..26217)
                     /locus_tag="ACG8VH_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9109852.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26285..28456)
                     /locus_tag="ACG8VH_001286"
     CDS             complement(26285..28456)
                     /locus_tag="ACG8VH_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9109853.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28667..29524
                     /locus_tag="ACG8VH_001287"
     CDS             28667..29524
                     /locus_tag="ACG8VH_001287"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9109854.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29595..>30381)
                     /locus_tag="ACG8VH_001288"
     CDS             complement(29595..>30381)
                     /locus_tag="ACG8VH_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9109855.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9079 a         6258 c         5380 g         9664 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt ttaacgactt agttatctta tcacgtctaa
      241 aaacatgtgt caactctttt ttggaatttt cataaaatgt ttcaatgaag cgcgacaacg
      301 ttgtttaata tatcattaaa aaaaataaag gtcaagctaa aatattattt aagcttgacc
      361 ttattataat ttcaacgatt gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata
      421 gataaatcac agcttttgaa acataagttt tgtactgctt cgattcgccc gaattctttt
      481 gcagttattt ggcaatgaga aaagcagaca tcttctatag gcttttcagg aaatgctact
      541 aaatcaaaag ctcgggaagg aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg
      601 atgtgtatgt ctttgacttt cgtcatttgc tttgattcag gtatttcttc agccactgct
      661 tgccaacttt ctggcatttg agcaaggtca ttgctatttt ttctattgta ttcattatgc
      721 cagtccatta tccaagaaaa aggaaattgt acatcaatca tattcagatt ttctaccctg
      781 atattttcga tcactccccc tctttctttt gaagatttca ttcgaatacc acagtctgac
      841 tgaaaaaaat cgatatcgtg aatatatacg tcactaactc cagcactaac ttcgctacca
      901 atagttacac cgtatcctga acggatttta catctagcaa cctcgattct tgatgatttt
      961 cgattgactc ttgcaccatt gccatctctg ccagacttaa tagcaataca atcatcacca
     1021 caggccaaat cacattcata gacacgcaca tttgttgaag aatcaatatc aatcccatct
     1081 gtacttggtc catcattgtt gcggatgatt actttttcta ctaatacatc gttagaataa
     1141 gtgatttgca agttccaaaa acctgatttc tgaaaaatca aatcacgaac aacaacatgt
     1201 tctgcatgat acagtaaaca agcgcgtgga cgtttaatag cataatccgc aatccaacga
     1261 agtccctttt gatcgtattc agctcttgtc ccgctttttt gatcttttcc ccaatacagc
     1321 tcccaccaat ggtctccttg accgtcgatt attccttttc cttctataag aatatctttc
     1381 gccgagagaa cattcaaaat cgctgctggc catttcattt ctacgccggc tactcgtgta
     1441 tcgatttctg gaaatgcttc gatatctgtt ctccccttga ggactgcacc ttcttcaaag
     1501 ataagatgac tcttatcttt taaaaaaagg gctccgacta aataaatgcc ttttggaata
     1561 aatacaggta agtgttcttt cgcaccttga tcgattgctt tttgcagtat tattgtatca
     1621 tcttgttgag tgtcgtttgt tttaaagttc aaagcattga taatcccttt ttttgtccgc
     1681 aatacgtttc tccctatctt tcattgattt ttttcaattc ttcaccaata aatacagttt
     1741 ttgaacgatt cagtgggtaa cctactagca aaatgattgc ggatacaaat aagcagaatg
     1801 ctggtaacaa gtttgccatc gtataaatcc tttcttgtac taaaactgat tggatggctc
     1861 cacctgttgt agacgattgg taaccaatga gtgccagcat aaatccgcct accgctcctg
     1921 cacatgcttg tcctactttt ctcgcaaaag aatttactcc atatactgtt ccatcttctc
     1981 ttaagcctgt cttatattga tgataatcaa tcacgtcagt aatgaatgcc cagaccatca
     2041 aattgaataa accagatcct aaagtagcga taaataaaag aatcagatat acccatgcat
     2101 ttgttatatg aaggaaatac atcagcaagt acatacatga agaagcaagt aacgcaaata
     2161 cgcttgcttc ctttttacca aataattttg tcagctttgt agcaaatggt gctaaaacta
     2221 ctactgttcc gtaagtaaat aaaagagcaa cagacattgc ttgtttatta tggaagtaat
     2281 cattgaataa ataagtcata ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt
     2341 caacaatgac taaaataatc agtgctcgat tagctaacaa accttttacc aactgagaag
     2401 cagatacttt ttcgtttttg acaactctta ctctttcagt agtcaattta caagttaaca
     2461 tatatgcgac aaatgctatt gcagcacatc cgattgaaat ccaaaagaaa tgattccctg
     2521 aaacgacttg ttgtccactc ttgttatggg tatacattaa aatcggaatg aagaatcctg
     2581 tagttgatcc gccaatcgca gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat
     2641 taggatttga gctaattgct gctgccattg ctccatacgg gatattgatt gttgagagaa
     2701 aaatcccata aacaatgtat gaaacaaaaa tataagcaat cttcaccgtc attggaagat
     2761 gttggacgaa tggcaaaaac aaaatgacag tcactaagca aaatggatac ttcatttttc
     2821 tgatccaagg aataaatcgg ccagtttctg ttaactctga gacatcacat aatcttccaa
     2881 ccatcacatc agcaaacgca tccaaaattc tcgaaatcaa gaataacgta cctacgattg
     2941 cccctgaaac gccaagaaca ttcgtataat aaatcatcaa gaaactattg actaaaccta
     3001 aaataaaaca gttacctaga tcaccgaaca tgtagcctat cttatcggaa atcccaaatt
     3061 cctttttttc ttgtttttat attcacaatg ttggcttttt attgcattat cttgacagat
     3121 aaaaaacaaa aaataatcta aaaacattga tgcataagat tgataacttt ttttacataa
     3181 caatatatac aaacaaaaaa attcactaac tagtgaaaat aagttagtga acctcattta
     3241 ttaaagaatt aaattacaga tgatcttttc catcatactt gaaaaactca aaatcagcat
     3301 aacttcctct ttttttattt agatcatgca ctgctatccc gataaaattt ccagtaaacc
     3361 ctcctgagag tatacacatg tttttttgcg gacctacttt cttccattct tcagttgata
     3421 cgtctttcaa atgaaactgt gctgtctctt tctttacagt gactttcaaa aaagtcttct
     3481 tttctgtgag tggaatcgtc caatcttcta tttgtgctac tccgttttct gattctatca
     3541 tacgtaggca ttttcccttg tcctcattcc aagtaacata acaataaata taattggatt
     3601 catttaagta cagtaccagt ccagccatct gattaaaatt attaggcatg tattcaaatg
     3661 ctgtctccgc atcaaaagta aaatccgttt gccgaatagc taacaaatga tgttcgaagg
     3721 tagattgaag agattcaccg gaaatcagac gtaaaaaacc tggtctacat tttgtatcac
     3781 accaatcagc atttggcaaa atgcgtctgg cattccagtt cttctttagc tctccttcaa
     3841 atgtatccat aaactgatga tctttttctt gttgtacctc ttcatttgtc tcaatcatta
     3901 tttcttccat cggagttgtc tggctatctt taagtcttaa ccaatcgtcg gacgtccaat
     3961 acacttcttg aatagcggtt tctcttccca aaatagccgc accgtttaat ggtctggtac
     4021 aaagatatgt catgtaccat cttcctgacg gggtttgtac taagctccca tgtcctgtac
     4081 attgcaatgt agaagcaggt ttgtcactag ctgtcagcat aggaaaacca ggatcaagct
     4141 cgtaaggacc agtaatattc tttgctcggc aaactgtaac tgaatgtcct cttcctgtac
     4201 cgccttctgc cgtcatcaaa taataatacc cgttacgaaa atacaaatgt ggtgcctccg
     4261 ttttcgctaa ttctgtacct gcaaatattt tatagatggg accatcaagc ttcttcttct
     4321 ctgcatcata ttcttgtaat acgataccgc tggatttatt cggtgtgtcc agacgataat
     4381 cccatagttc attgacaagc catttttttc ccgtttcaag atcatggaac aaagaaggat
     4441 caaagccgtt actatttaaa taaattggtt cggaccaagg acctgtgatc tcagatgcag
     4501 tgatcaaata attgtggaca tctttaaacg gacggcctgc acttttgaca tctgtgtaaa
     4561 caagataaaa caatccatca gcataactga tctgtggcgc ccatatactt ccatttgtag
     4621 gatttccttg caaagatact tgagtggtca aaatatccgt tgtatgtgac cagttgacta
     4681 aatttttcga ctcatatact cggattcctg gaagccattc aaaagaggag acaataatgt
     4741 aataccgctc tttcactctt aatatattag gatctggatt gaatcctctt aaaataggat
     4801 ttttcacttt gatagtctgc atgattcagg ctccttttct cattcttact cgatataaac
     4861 tgaatgcccc agctgcgaaa taaaaaaatg caaaaggaaa cgcaataaac tgagtgccaa
     4921 tcaacaatat gaatcctgtt ataaaataca aaccagtggc agttgttaag tattttcctt
     4981 tagcagtcaa aaacaatgct actgctaaaa aagcaagtga aataaacaac acaacggcaa
     5041 acagaccagt catgtcctgt aataatccaa atgtattttc taaagacaaa gttgaatgat
     5101 ctacagcaag cgactgataa ctttttttaa actctgacgc agacatatta cttaaagtaa
     5161 ctgctaaccc gccaaagaaa atcaaagata gactgccacc gaccattcct agcaatagtt
     5221 cccattttct tttcatttct gaaccccctg cttttctaaa tgtatccatt caggtctttt
     5281 gatcgatgtt tcataattgt tattacgttc agagaatttg acagtctgtt catttttctt
     5341 gtcatcccaa ttatcccaac cagttgggtg aacatgccca cctatcttac acgcttcaaa
     5401 taagacattt gcatacggac gccacggtct tcctaaatag acgtttctag tatcaggttc
     5461 tgcagtcaag gtacagcttt caaaacgata ccctgttttt tgatttttcg gcgtagatgc
     5521 tgctgtgatg tatccaggat ttttactggc taagctatgg atatgacact gatgaaaaac
     5581 ggcatctgcc ccaccaaata tgaaatccac cgttccttca atccagcaat attcataggt
     5641 ctgacgacaa tactcaggat tggcagaagc cggtgtacaa aaaggagtac catctttttg
     5701 caaatcaggc aacggacccg tgcataaggt atcctgataa ccagatagtc gacagttttt
     5761 taaagatacg cgatctcctg aattgaataa agcaacagcc tgtcctacct tttctcctgg
     5821 acctgctgta ttttcgatgt gaagattttc taatacgaca ttcgatcctt ctaagaaaaa
     5881 agtagcactt cggaaagtcc ctaactggct accgtcaaga tactcctgtt ttgcaaaacg
     5941 attaccggag ataactactg ttcctgctcc cacaagttta aaattggata atctgctttc
     6001 tatatattca tcatatcttc cttcttttac tatcaagacc tttggatggc tatccggcag
     6061 gcctgccaaa taatcgattc cttcttggat agtttcaaaa tccgtcgggg tatctatagc
     6121 attctttcca attatcagtt tgtctttcat cctcataaat gctcccttat gaattgtttc
     6181 caaatatttc tcatttcttg tgtttccata tgaccgcctg ttaaagaaaa agaagaaaag
     6241 ttttctggac tacccattga ttgatacttt ttgtccagcc cttttctcag cttcagtacg
     6301 ccttcgtcca agcacatcgt atcgtttttt ccaactaagc taagacgagg gcgcggagcg
     6361 attttttctt ggattgctaa cgttgtataa gctgtcaata gatttggcac atagtaataa
     6421 aacccatgat gatctaggcg gcgatgtagc aaaagtattt cgatgtccac ctgtgccgct
     6481 agatcaatac aaactttgac acgagaatcc aatgctgtca cccaccagct taataagcct
     6541 cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg tgtcttccct tgtctccaaa
     6601 taatcgatca agctgatcac atcataaata cgcattcccc ataacgtttt tcctgttaat
     6661 aaaaattgtt tgaacaactc gctttcagaa atacctccgc gttcttcaaa tccccaagca
     6721 tcaatcgacc aaacagcata tcccattcct gtcagtactt ctgcaaaaga tggagaagct
     6781 aagtaaggag caccatggat caattcactt ttcccgactg aaaaatctcc gccgtgagaa
     6841 tgcaggtaaa ggaccggcgg aaacggtcct tctttttctg gataagcata gagggctggt
     6901 actgtttcca attcatttaa gtacaattga tagtattcaa gcataaaacc gttttgtttt
     6961 tctcggtata atcgttttcc actaggggta tgtctgctag atggagcacc taaaaaatca
     7021 ttcaactgca taagcaaagt ttttacgaac aaccagttca tccatatttt ctgattgatt
     7081 tgattggaat ttctcaatac tgatttcttt agtattttct aatagaaatg ctggtccctc
     7141 gtgattttca atcgacatct ggttaaagga aaccttaccc gcaaagccaa tataaaatcc
     7201 ttgtctgctc atttcctcta ttcctgtcat catagcagga acaccgggaa ctgcatcttg
     7261 ggccatcgag acatcgatat tgtcaaatgt gatttcttgt atgaattgtt ccgctaatcc
     7321 ataaataaaa cctgcagaag catggacatt ccttgccgtg atattcgaaa aatggatcct
     7381 tctgaatgct ggtgtccgtt catctatcgg ataagccttt ttttcccata catacggttc
     7441 tttccctcgc ggaccacaga aataataaag attcagtata aatggacaca tcacattatc
     7501 catgactata ttggatactc gaatatcttc aacgatcccg cctcgacctc gtctagattt
     7561 caatcgtatt cctcgatcag tttcttggaa gatacaattt gaaatggtaa tattacgaat
     7621 actaccactc atttcacttc ctaatacgac accgccatgt ccatggacca tcgtgcaatt
     7681 tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta tcttcagtcc ctgccttgat
     7741 tgcaatacag tcatctccca cgtcaatatg gcaattacta atacgcacat ttttgcatga
     7801 ttctggatca attccgtctg tatttggtga atccgctggg ttcaagatcg tcaagttatc
     7861 aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa cttggtgact ggatcaattt
     7921 aatatctttc acagtaattc gatgacagtt atggaaactc atcaatttag gacgaggata
     7981 agcgagatta tctggttcat ttctaaaagt atgccaccat ttttttccat ttccatctaa
     8041 tgtaccgaaa ccagtgacag agatgttctc tacattttga gcatagatac aggaagcata
     8101 aacttttcga tggactcctt cccaacgtga atgaacaaca ggataatctt ttggatcgtc
     8161 agagaatttc agaaccgctc ctgcgcttaa atgaagttcg acattgcttt ttaaaaataa
     8221 agctcctgtt agaaattcac ctgctggaac aactacggtc ccgcctccgt cacttgctgc
     8281 agcatcaatg gcttgttgta ttgcttcagt attcaattca tcaattgatg cgccaaatgt
     8341 caaaatatca tacatattta taatgcctcc acttcaatca atgcttgcaa gaatgcacca
     8401 acgccttttt gatcattaca aataattggt tcactgatgt agtaagcata tgaaccatct
     8461 cgctgatctg ctccgcctaa ccctgctact tgacagtttt tattcaagtt cagccagcct
     8521 tcttttgttt ccagaacaaa ttcatcaagt aatccttgat gtgatttctt cacttgtgga
     8581 agccattctt ctttgttcag cagtcccaaa cgtattcctt tagccattgc gtaaacaaac
     8641 atactactgc cggaagcttc cagataattt cctttccgtt ttgtttctgt tgttacttga
     8701 taccatgtcc cgctgttttc ttcttgatat ggaagtaatg cagccagtgt cttttctaga
     8761 atttctttca gcaaagaaca atctgtttct tcatgaagta attcataggt atctgctgca
     8821 gccatcaaat accagccaat cgatcggctc caaaaatgat gggatagacc tgtttttgga
     8881 tcagcccatg gttggacctt cttttcatcc catgcatgaa tcagcaaatt tgtttttgga
     8941 tcgaatagat gtttataact gatttcaaac tgtctcaaaa catcttggta atctttttct
     9001 tcactaaaat tctggataaa ttcagcataa aaaggagaac ccatatataa gccatccaac
     9061 caaatttgat aaggataaat ttctttatgc caaaaagctc cttcggatgt gcgtggatgg
     9121 tttgccaact gctgggaaag gagttctgct gcttttttat atttttcttc gcctgtttct
     9181 ttgtacaata aaaagaaaag ctttcctgta tttacatggt cgatattgta ttcttcaacg
     9241 ctgtagccac ggatcgttcc atcttcttgc acgaaatagt cgatattgtc ttttatgaaa
     9301 tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt ggaacccttt aagtacgacg
     9361 ccatagtcat aagaccattt tcctttatac cacttttctt catataagcg aggtgttctt
     9421 tccatgatac tttttgctaa tttctttgac cagttttcca ttatttgctc tcctttaaaa
     9481 attaaaatag gatttgggac aaataccaag aagtatttgt cccaatcgtc tatattactg
     9541 tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt cgatcgcctc cttgtgcttt
     9601 ccagtcttct acagcttttt tgaatccatc ttcgtcaagc tgtcccatga tgtattgcac
     9661 cgtagcatca atgacgattt tttcaatttc tgatcctact tcattgttcg tcggtgaatc
     9721 caatggcaca gaaggatcca ttacagcaaa ttcttcattt tctttgatca attgattaga
     9781 gaattctttt tcagggtctg tatctttgaa agagaacgtc acttcactag gacgagagct
     9841 tgataacggt tgcacctctt gttgccaaag atccatatcg ataaattcga tagaaccatc
     9901 atcattcaat tcataatgcg ttccttcgat accgccagtc atcaatttat acacatcttc
     9961 ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat tgtgcttcgt cttttacttg
    10021 agatttggga aatgctagca agccgccaat accatttcct tctgaccaaa catgataacc
    10081 gccgccttca ccatctgaga tcttgtttac aggcactaat tccatatcat cttgaatacc
    10141 ttgacccatc gtcttcagat tcttgatatc caccattcca gtgtagattc cagttcttcc
    10201 ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact gcaaagtctt gtgccaaata
    10261 gccattctca aatagatctc ttgaaaaatt caaagctttc atatactctt ctgtatcaaa
    10321 ttctggagtg aatttgccat tatcgtctac agcccagcca tttggtgctc caaaataaga
    10381 agtgaaaagt ttaaagctgg tataacgaat atcactaggc gcacgatcgc caaaaccaac
    10441 tgtgtcgtcc acaccatttc catcaggatc gttttctgta aattgtttag ctacttcata
    10501 aagctcatcc atggtagttg gaacatctag tcctagatta tccaaccagt cttttcgaat
    10561 gactaagcca gcacgagcgt agtctttttg tatcggtacg ccgtacaagg caccgtcgat
    10621 actcgcagct tcgattctat cttcagaaat ttgacttaaa tttttatagt cttttaaata
    10681 ttctgataca tcccagaata cgcctgattt taacgcattg cggacagaag agtttttcaa
    10741 catcgcaatt tgaagtgtaa cgatatctcc tagttcatca gaagccaatg cagttgttaa
    10801 tctttcttct tttgaagcat caggaatcca gttgaatgtc agttccgtat cggttttttc
    10861 ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca tgatgcaaaa tatttaacca
    10921 agtgatttta gcttttccat cttctgtctc tgaagaactg ttacttgaac tgctgccgca
    10981 tgcggcaagt gctagaattc ctgcaaaact aagacctaat tttacgagcg ttgctttttt
    11041 cataaatttt ctccttcact gttttaataa tatttttgtt ttcttctgtt ttttctgtat
    11101 atgagttctt ccattccaaa tggcaaaata tagaatttgc tgtccacgtg actgcggaaa
    11161 agaagagact gccaccgata aaggttaaaa cacctggcat tgtttggatg atcatcagat
    11221 aaatccctac catacttaat atcaacacta atgagtaatg aagatgtgag aatccgaata
    11281 aaaatgccat tttcattttg aaaaaatatc ctttccaatc ataatgagcc atgattggga
    11341 aaatatagag tgctgagagc acatatatga acgcagcaat atacaaagcg atcctaagat
    11401 accaattgtg tatgaaagaa agatccacaa gtaaaacaat cccgatggcg ccatatatcc
    11461 agctcatcag caaggtttca cgatagtttt ctttaaaata tgtgaagtac gttttcgtca
    11521 aaggaaacgt ttcttttgag cgcagccatt ttctaatcac tgcaaccatc gcatacgatg
    11581 caggaccgaa agtaaaaact cccaaacctg cgatgactaa accgccccac agaaaattca
    11641 aataaaccaa ctgtaaaata cgatttagcc ttccgtttat tttatctaat gactgcagca
    11701 tatctttccc tctctttcta tcttagtaaa cgccgtcttc tcctagcttt ttggcaatct
    11761 tattagctaa aattaccata aacaatccta cgaccccttt aaacaatccc attgctgtac
    11821 tgaaacttaa ctgtccattc ttcaatccag ctgtataaat ataggtgtcg aaaatttctg
    11881 caacgcttcg attcaatgaa ttcaataaca agtacatatg ctcgaaccct aagtctaatg
    11941 tctgaccgat ttttagaatc agcaaggtaa tgattactgg acgaattgcc ggcaatgtaa
    12001 catgccatgt tttgcgtaag cgtcctgctc catccatttc tgcagcttca tacaactgtg
    12061 tatcgactgc agttatcgct gaaaggtaga tgatcgtaga ccagcccaac tctttccaga
    12121 tcacttgtcc gatatatact gtgcgtgtcc attcagggga tgttagaaaa ctgatttttt
    12181 caaatcccat tgttgcaagg aattcattca atacccctcc gtcaacattc aaaaatacat
    12241 aagtaatcga aacaatgatt acccaagaca tgaagtgagg gatgtaaatg atcgtttgaa
    12301 tcccgttttt caatcgtttg ttcgttactt catttaatag taaagacaaa atgattggca
    12361 tcggaaaaaa cacgaagata tttaatccga acaagatcaa cgtatttctt aataatgtaa
    12421 agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa accgacccaa ggacttccca
    12481 ttattcctaa aaatggttga taatcttgaa aagcaataac gagtcccccc ataggaagat
    12541 acctaaaaat caagaaataa aggactcccg gcagaatcat cacatataaa aatttattcg
    12601 ttttgattcc tgcaattatt ttttttcgtc ttctgagctt ttcttctttt ttcgatagcg
    12661 tcagttgaaa tggttcttgt ttctttacac tttccataag cccctcctta gttttgtgaa
    12721 tgtttgctgc ttatgacttc atgatagata actatctgaa ttacgtatat aatcattttt
    12781 accgtaagta aaaacgctta catagcaatg atttttgtgc tatacaaaac gataaagtgt
    12841 attttttcac aaataaacta cgcatagcaa accttctatt tttttctatc tgacacttag
    12901 cataggaaat gattatatac gtaatctttc accgatgata aattgaacat atagaaacag
    12961 aaacatttaa tttattttga ggaggagcct atctatatga aaaagaaagt ctctatagga
    13021 gaaagaagtt ttacactttt caacaatacg tttctaattt tgctagcttt gatctgtatc
    13081 gttccttttt taaatatcat cgctacatct tttgcttcta cacaagaagt cgtcgcgaaa
    13141 aagtttatcc tttttcctac taccttttct ttagacgcgt atcgctacat tctttctacg
    13201 ccgaccattt ttagagcact tgctgtttcg attggtgtta caggtgtcgg aacgatcgtc
    13261 agtatgtgtg caacttcact gatggcctac ggtctttcca gaaagtattt gtttggtcga
    13321 ggtttcgtca actttcttgt tgtattttct atgttgttca gcggggggat gattccaacg
    13381 ttcttagttg ttcgttctct agggctggtc aattcttact gggccatgat cctgcctgtg
    13441 gctgttaatg caatgaatat gatcattatg agaaactttt tccaagcctt gccggatagt
    13501 ttggaagaat cagcaaaaat ggatggttgc acagattttg gtgtattttt caaaattatg
    13561 ctgccattag cgttaccgtc tattgctact atctcgcttt tttatgctgt tacttactgg
    13621 aacacttata tgactgctat tttgtatatc aacgattcat ctaaatggcc gatacagatt
    13681 cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag cagaaagtag tgccgttgat
    13741 gtgattccac cagcacaaac agttaaaatg gctgttatcg ttattgcaac agtgcctatg
    13801 ctgattgttt atccttttgt tcagaaatat tttgtcaaag gagcactcgt aggttctgtc
    13861 aaagggtgat tatgtactga acgcaaaatg gtagttgtgt cagaagtggt cagcttcaag
    13921 aaataagaag gcatttacga aaattgcttc ttaaattttt gtgaaatttc ggcttatttt
    13981 tcgaagaatc tactcctgga acaccgtgta tcagaggttg tgaaagagac gttcagcctt
    14041 gagcgatgat aaaaactctc acaaacattg cgacgagtaa cccacaggag caataagaag
    14101 cgattttgaa aaatagcttc ccatatttca caaaattatg ccttattgtc gatagcctgt
    14161 ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa gacttttgtc acagttcttt
    14221 ttgcgtgctt agccttgaaa tttaactatc cataaataca aatactaggg ttattctaac
    14281 ttcttttatt gcttgaatca gtccacaaga aaaacagcct cttgattcac aaatattttt
    14341 ttcgatattc tcccggcgta atcgattcct tctttttaaa gaaacgaata aaattttgcg
    14401 gatttcgata ttgcaatctt tctgcaatct cttttacgga gaggttagtc tctttcaacc
    14461 atttttttgc tacctctaaa cgatgatttt gaacaaaatc accaaatttt tctcctgttt
    14521 cctttttaaa tacattactt aaatagttag ggttataatg caacctatca gcgatcacat
    14581 ctaaagacag atcacgatca tattctgtgt agacgatatg cataatattc tccgacaaat
    14641 ttttaaattc atgatcagtg attgcttgag agcattctgt gattggcaga actaattgtt
    14701 ctaaaacaag tttttctagt tctttgggat gataattgtt cagtgctttt acataaattt
    14761 gtttcatgtt atcaaagttt tttgaatcta ctcccaataa ctgtcctaat tgaacgagtt
    14821 cattgatcaa gcggaccgaa actacttccc gactcaaagg atttttattt tgtgcaaaca
    14881 atgcatcaat caattcatgt actaaattct tcacttcctg atttccagaa cgaatggctt
    14941 caaacaatcg attttgttct tctatcggat atctaataag tgttttttca tgagcagcag
    15001 gaacgatttc ctgataaaaa ataattgaac ttggaccagt atttacacga taatacaggg
    15061 cttcttttgc ccgatcaacc gattgcttgc tttcattcag cgtttcgaac cggttactga
    15121 tgcctatact gatagttaaa ttcaagtatt cttttacacg tttttgaatg agtcggcaat
    15181 actccatgac ttttttgctt gcttctgcat catctttatc cattcgataa atagtggctt
    15241 gcatttcttc attcaaaacg atcggcagca ttcgattttc ctttggcaca atttccgtga
    15301 tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc accgtgacta tcttctaaca
    15361 gatcgatttg aaccaaagct gtataatagc agtcattttg agaactataa ccaaattgct
    15421 gcaaccgctg atctagttct cgcctttcta ctcgatttct aaataaacta agtacaaata
    15481 gcgtttccag ctgtggcttt tgggtaacta agtgtgcact taacgcttct ttttctccta
    15541 tgatccgact gactgattga gcgaccaaat ctagttcttt cccactgaat ccttttgatt
    15601 taagattcag acgttcctgt atttgagaaa tcggacgggc aaatctgtcc gaaaataagt
    15661 aggaaagaag accaatgagt cctaccagtg ttaaagacac tgcaactagc ccgattctca
    15721 gattgcggat cattgcgcca attgcactag agtcaacttc taatctgtaa atccaattat
    15781 tataatctga tttcagtact atatacgttt tatctttttc tcttatcact ttcagctgat
    15841 tgtaatctag atccacgtcc gttaatacag ttggttgctc tttatttttt actgtctgag
    15901 tagtaaaaag gttgccaact tcgttttcaa tagaaagtat gtcttctcca ctttcaccaa
    15961 caacttgttc tattgtatgt ttctctatcg tagcaattcc caatgcatat ttttcttttt
    16021 caaacattgg caaagaaatc accatttcga ttcctttgtt tttcttttgc caaaataaat
    16081 tatttttgtt atttatgtac ttctgttgat acttttcaat ttcctcttcg gtcagttgac
    16141 tcaacgatcc attgattact ccccatttac cagccaaact gactaaatca taattattat
    16201 catccataac aatcgtttct ataaaattta gttctttcct gatttcagaa taagtggagt
    16261 aatctgtata tgtcagcgga tgcttgaata tgtccgagaa atttttggtc gaaccatacg
    16321 tagaaaacga atattcaatc gttctaattt tccattcaac gttagtttta acttgcctga
    16381 gcaaattctt tttgtctgtt aaataactcg attcgatcga ttcataggta ttctggtaaa
    16441 tgaataaact aaatcctatg acaagtacgg ctcctaataa aaacatcggt aaaaatattt
    16501 tataaaatac tgtacttttc ttcattttct tcctctcctc cttttaaaat aaaaggcttt
    16561 catttttctg taaaattgat actcatctaa ttaaatgagc attccccgct ttcaagaata
    16621 gcataattaa aaataaaaat ccaagtaatt tttaacgtgt gcaatctttt catatcaaag
    16681 atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa agtgaaaaag
    16741 taaggaaaat gatggaatgg taacgatcaa agatgtcgct aaagcagctg gcgtttctca
    16801 tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa caaaacaaaa
    16861 aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa aaggtttagt
    16921 gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag gaacttcatc
    16981 aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa attacagttt
    17041 atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac gctacgatgg
    17101 actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc tcaaagaaaa
    17161 agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta tcaacgtcac
    17221 tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat gcggacatac
    17281 gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac gggaaaaagg
    17341 ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ccagcctatt ttttaacagg
    17401 agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac tttctgacct
    17461 gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga gagctgtaca
    17521 tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg attctccagt
    17581 cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag aaatgtgtca
    17641 agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac aatctaaaat
    17701 tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa ctacaagtaa
    17761 ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat gaaaaaactg
    17821 acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt tggtgaaggc
    17881 aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca acaattgttt
    17941 aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat gatgaaccag
    18001 caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat cgttgatact
    18061 tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg ggatgcttat
    18121 ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac agaggcagga
    18181 atccaattcg tggagactga taatttaaca gatactcccc cacagagcct cccgggcaaa
    18241 ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac catcattcct
    18301 tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg ctacgcagaa
    18361 aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt tttctgttgt
    18421 agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt tttcaatcaa
    18481 cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct ttgggtgatc
    18541 gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt gaatgtcatc
    18601 atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa tggaccgcat
    18661 acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga agtaatgaaa
    18721 gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat tcctatgctt
    18781 tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg tttcttgaat
    18841 ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa gttcagcacc
    18901 cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc accgttaatc
    18961 actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa gccacatgat
    19021 gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac agctattcct
    19081 cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc gaatgtgaca
    19141 gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc agtattacaa
    19201 gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga aattaaatcg
    19261 acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac tgacgattga
    19321 tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg cattgggtga
    19381 tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg tgacgacaga
    19441 cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt ctggtgaatt
    19501 ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga accgaacatt
    19561 tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt ttttagtacc
    19621 taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca aggaaaaaca
    19681 tccagatctg ggacagtttg accatattac gattttaaag catccttatg gctgttcgca
    19741 gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa atcatcccaa
    19801 tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc ctgaattcaa
    19861 aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc aagatgttta
    19921 cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg cggaaaatga
    19981 ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg gcggttctga
    20041 tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact acttgattgc
    20101 tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag aacaagtatt
    20161 gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga tcaatgactt
    20221 caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc ctggtaataa
    20281 agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa aatcaggaag
    20341 ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag ggcttagttt
    20401 attgcaagct cccgggaatg atcttgtagc tgcctctgct cttgcttcat ccgattgcca
    20461 gttggtcttg tttaccacag gaagagggac tccgtttggc tcatatgtcc caacggtcaa
    20521 ggtatctacg aatacaactc tttttgaacg caaaggtcat tggatggatt ttaatgcagg
    20581 cgtcttatta gaacaaccaa tggaagtaat cttagaatcg tttgttcaaa aaatcatcgc
    20641 tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac gttcgagaaa ttgccatttt
    20701 taaaaacgga gtaacacttt gattttcata aaacagtata aaaagaaaga aggaagattt
    20761 atgtttttat ctgatgattt tttattaaaa gacgattggg cgaaaaagct ttaccattcc
    20821 tacgcaaaaa acatgcccat tattgattat cactgtcact tagatccaaa agaaatttac
    20881 gaaaacaaca atttcagcaa tctgacagaa gcttggttag gaggcgatca ttacaaatgg
    20941 cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca caggaaatgc ttctgatttt
    21001 gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta tcggaaatcc gctttacagt
    21061 tggtcacatc ttgaattgaa gcgtttcttc ggtattgact tgttgataaa tgaagaaaat
    21121 gcagagaaaa tttggacaca agcaaatgaa attttggcag ctcctgattt tcgccgtcgg
    21181 gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa cagatgatcc cgcagacgac
    21241 ttgcactatc atcagttgct tgctcatgaa gaaccagatt tgaaggttct accttcattc
    21301 cgtccagata aagcactcaa tattgaaaaa gaaggattta atgcatgggt cacactactg
    21361 gaagaagcag ctgaccaaac gatcaccacc tatcaagaac tgatagaagt cttagggcag
    21421 cggatagaat acttccatca acatggatgt cgtgtttccg accacgcttt agacatctta
    21481 cgctataaag aagcggatga aacagtatta gaagagattt ttcaaaaagc acgagctaac
    21541 aaactattat cttcagatga aatcgatgct tatcgtacag aaacactgat acatttgacc
    21601 cacttctatc attctcataa ttggacgatg cagctgcaca tccatgctta tcgtaactgt
    21661 aatacacaaa tgttggaaaa attagggccc gataccggct atgatggaat gaatgatctt
    21721 tctttgacta ttcctctgca aaaattattg aaccgtgcag aagaaacgga tcagttgcca
    21781 aagacgatcc tttattcatt gaatccgaat gactatcctg cgattttagc tttgatgggc
    21841 tgtttccaaa aagaacagaa tggaaaactg cagttaggtt ctggatggtg gtacaacgat
    21901 actcgagctg gtatgcgcga tcaattgaca caatttgctg atggtagtgc tttggggaat
    21961 tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct acactagaca tgaatatttc
    22021 cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc gaggagaagc acctgaagat
    22081 gaacaacttt taggaagtct agttcaagcg attagttata caaatgctaa aaattacttt
    22141 ggcttttttg cctaattgat taatagtaac agtaacgaat caaaaacagg atagtgaaat
    22201 aacggaatcc gtttatccac gttttagaga agaagacgga ccaaatttgc tccgtcttct
    22261 tctcttgctg ttctgcttca ttgcctataa acctttatga aagaagagat aacatgccta
    22321 ctatccatat tgccggtgat tctactgcgg cagaaaaaga tgaaaacaag cgtcccgaga
    22381 caggttgggg agaaaaaatc tctgcttatt tttcacccga gatccagatc aataaccaag
    22441 caaagaatgg acgcagcacc aaatcattta tccaggaagg aaggttagcc cgtctagctt
    22501 cacagtttca gccgaatgat ttcttatttg tacaatttgg acataacgac cagaaaatcc
    22561 aagaagaaag aggtacgctt cccttcggta gctatttaaa aaatctgcag atattttttg
    22621 aatgcgcaaa aaaagcgaac gtccaaatgg tcttactgac atctgtgacg cgacgagatt
    22681 acttagaaaa cggaacattg aatccagata tacttggaga ctatccaaaa gcgatgcgtg
    22741 catttgctga gaaacaccat attcctcttt tagatgtgtt ctcaagatca caagaattgt
    22801 tccagacctt ctcaaaagaa gagacaaaaa aattctacct gcatcttata cctgatactt
    22861 gtaaaaatta cccagaagga ctaaaagaca atacccactt tagtccagaa ggcgctgata
    22921 aagttgcccg aataattgtt gaattgataa aagaatcccg tctgcctctt gctaattatt
    22981 tacaaaaagg agtttgagat gtttccatta aatttaggca tccgtgccca cgatttagat
    23041 gttcgaaatc gtgaagaaat cgttcaaaaa gtatcacagc ttcacttgtc ccatatccaa
    23101 tttgcaccac aaaaagcatt tcctgaacaa cttaaattaa ctgacatgac acttgggagt
    23161 gctgcctatt ttggtgaata cttccaaaaa aacaatatcg aactttccat tttaggatgc
    23221 tatatcaacc tatccagcaa aaatcaagaa gttcgtgcac aagcattggc aactttcaaa
    23281 cagcaaattg ctctgtgctc tgcctatcaa gccaagatga ttgctacgga aaccggtagt
    23341 gtctctgtcg gttacacaaa agaaaatttt actgaagaag cttatcagat cgccagaaat
    23401 tctatcattg aattagtcgc tcatgcagaa aattttggcg taactgttgc tgttgaagca
    23461 gggatcaatc atccaatcta taactatcag ttagctaaac gtttaatcga tgaagtagct
    23521 tcaccaaatt taaaaatcat tttagattgt gcgaatttga tgcataaaga aaatcatgct
    23581 gatcaggaaa aagtgatccg aggtgccttg gacaatttac atggttatat tacagcgctt
    23641 catctcaaag actatatcat ggaaaatgga aagatccgca tggtacctgt tggcaagggc
    23701 tgtttagatt tccgcccggt actgcattat atcaaatatg agcgtcctct aatgtacgcc
    23761 actcttgaag ctactcctga gatttatgtt cctgaagcaa tagaacactt gcagtttatt
    23821 taccaagatt gttaaggaga agattatgga ggaacgaata cagaaaagtt atcaaaaata
    23881 tcatgcaaat gcttttcaga cattgctgca tctttatcag ggaaattatt ggcgttttgc
    23941 catgtcagga ctctttttta ttatcaaaca tcttccatct tggttaatgc cgatcgctat
    24001 tgccaatgta atcaatgcag cttcatcgaa agattcttct cagttgcagg ccatttattt
    24061 caacgctttt ttgatggctt gtctgattgg tcaaaatata ttgacgaatt attttcatgt
    24121 aaaataccat agcacttcga tccgtcaagt agaagctgga ctaagagcga cccttatcaa
    24181 gaaaatccaa gaactatcta ttccttatca aaaagaattg caatctggac gaatccagtc
    24241 aaagatcatc cgtgatgtag aagctatcca aacgttgtcc tctcaagtat ttgtcagcgg
    24301 attaaatatc gctgtcaatt tattcgtagc tttaggtatc acgctttata aaagtcctgt
    24361 tgttttcatg tttttcttat tgactgttcc tctcgccgca ttcgttactc tctttttccg
    24421 taaaaggatc agacagtcca actcagactt caggcaagaa atggaagaga cttctgcaaa
    24481 agtgatcgaa gtgatcgaat tagcaccgat tgctcgagct catgggttag aaaaacacga
    24541 aacaactcgc ttaaataagc attttactca aatctatgaa aaaggattcc acttagatat
    24601 cctgcaatct gttttcggat cagtcagctg ggctagtttt cagatatttc agctgatttg
    24661 tttgattttc actgggatat tagctttaca agggcgcatc caacccggcg atgtcgtgat
    24721 gtatcagacg tatttcacga cgatcgtcaa ttccgtttct ggaatgatca cgctgattcc
    24781 tacgatttca aaaggcatgg aatctatcac ttctattggc gaactgttga ttgctggcga
    24841 tgtagaaact tatcacggta agaaaaaaat ccaacatttg gatggaaatt ttcgttttga
    24901 ggatgtttct tttctttatc cggactcact caaaccgatt atcgatcatt tttctctaga
    24961 ggtgaaagct ggagaaacaa ttgcttttgt tgggccatct ggctctggaa aatctacact
    25021 ccttaactta ttgattggat ttatccagcc gacttcagga aaaatttttc tagatgatca
    25081 gcctttggat gaactggata tgcgaagtgt gcgaaattat ttagccgttg ttcctcaaac
    25141 cactttgttg ttctccgctt cgatcaaaga gaacatcact tatggcttga aaaacgtatc
    25201 aaaagaacgt ttagatgaag tcatcgaagc tgcgcaactt tcttcactga ttgaccagct
    25261 tcctgatggt ctggatacct tagtaggcga acatggcaac aagctgtctg gtggccaaaa
    25321 gcaacgtatc tccattgcac gagcgttgat acgtcagcca aaaatcattt tgctagatga
    25381 agcaacctca gctttagata accagtcaga gaaaaaaatc cagcaagccc tgaattacct
    25441 gacagaaaca cccactactt ttattgtggc tcatcggctt tcaacgatca aagaggcaga
    25501 taaaatcgtt gtgatcgatg aaggaaaaat cgttgaaata ggtacttacg aaaaactgtt
    25561 agctcaaaaa ggctatttct accgcttgaa aaatgaatga caaaaaatag gagctatgac
    25621 aagacttttg tcgtagctcc tatttctgaa taaacggtgt tctcctgcca actcctcggt
    25681 cacaagtcac aatagtcatc aaacatgaga agctgttttt tgaaattgtt ccttgtaact
    25741 cagagttaaa cggtaggctc acgacctctt ggtaaatggt gttcaagaga cagcctatcg
    25801 gcaataagaa gcaattttgt catcgctcaa ggccgaacgt ctctttcaca ccctctgttg
    25861 tttcataaaa tagttttatt cgctgtaaag gtcgcgaact ggtgtcatga tgatacggtt
    25921 caagtcattg atgattgtac tgaagccttg ctctttttgc atcaagtcat tgattacttc
    25981 ttctgattgg attttcacag ccatttcttg tgctttcatt gcatcttcgt cgctgaactc
    26041 ttgtccactc atttgttttt cttgtaattc gatttgcatc gcttggaagt ctttgaacag
    26101 tttgtgtgct tcttcgtttg cttgtacttt ctcgtacgct tctttcaacg ctttgaattc
    26161 tggtaattca cggatctcac gttcgatttg attcgcgcta tcgtagatgt ttgacatgtt
    26221 atgccctctt ttcttttagt agttactctc tattgtaaca tggggttatt cgttttcaaa
    26281 ggggctactc tccttgatat ttccgccaga ggtctttagc cgcttcacca attccctgaa
    26341 cgccttcttt caccatatcg ccgaagtttt tcagcccttc ttttgcttgt tcaccatatt
    26401 gattcaagtt atcttgccaa tttccagact ctccattaga atttcctgtg tttccaactt
    26461 gatctgcagc taccacttgt cctcctgtgg cataggcatc tgctacacta aatcctgttt
    26521 ttgaagtata cggaaggatg ccttctgccg ctgatttgaa gactttcccg acaacatttc
    26581 cacttgtccc ttccaaatag tgcagcttgc tggattcttc gtaacccatc catgtcgaaa
    26641 tcactacgtc tggcgtgtaa ccgatcatcc attggtcatt tgcttttgtg gcatcgaaat
    26701 tagtttctgt ggttcctgtt ttccctgcta tcgtataacc tgcaggattc gcagcagccg
    26761 ctgttccatt tgaaaatgta ccaagaagca tgctggtcat ttcatcggct gtttcctttg
    26821 atatgacctg ttttggtttt ttgtttgtgt tatcgacgat caccgctcca gtcgaatcaa
    26881 cgattttcgt gataagatgc ggcgtataca tctgaccatc attggcaaat gcactgtaag
    26941 cacccgccat gatcaatgga gatactcctt tttccagtcc accaagggct aagccataat
    27001 acttatcaga ttttgctaaa ggcaggccga attcttctgc tttatcatat cctttttgta
    27061 aaccgatctc atgcaaaagc catacagtag gcaaattcaa actttgtgca acagcttgat
    27121 acatcggtac ttctccttgg tatgtcccgt caaaattatg cgcatcgtaa taactttgcg
    27181 gttcgtcttt caagatactg gaaggcttgt atccagcttc cagtgctggt gtatatacgc
    27241 ttaatggttt gatcgttgat cctggtgaac gacgcatctg tgtagcaaag ctgaatcctc
    27301 taaagacatg atctcctctt cgtcctacta gtgcttgtac accacctgta ttcggatcta
    27361 aagctactga agcagattgg accatggcac catctgaagc atttgctggg aataatgcac
    27421 tgttttgata agtagcttct aattgctttt gataatcctg atctaaagct gtatagattt
    27481 tatagccttt attcatgaca tcttcttcat caagaccata ctcgttaact gcttcatcga
    27541 tcactgcatc aaaataataa ggataacgat aaccagaatc tgaattatcg tatgtgtcat
    27601 taagatacgc gctgatatct gtctggcttt ctgcttgttc ttgttctttt gtgatttttc
    27661 cgttttcttc cattacactt agtactgtgt ttttccgatc attcgcattt tttgggtgat
    27721 cgatcggatt ataaatacca ggacctttca gcattcctgc caaagtagca gcctcagaca
    27781 gcgtaacatt cgttgcatct actccaaaat actttctgga agcgtcttgg acaccccata
    27841 ccccgttgcc aaaataggca ttgttaagat acatggtcaa gatttcttct ttgctgtact
    27901 tcttttcgat ctcgattgcc aaaaataatt ccttcgcttt tcgatcaaac gtttggtcta
    27961 atgtcagata cgcatttttg gcaagctgct gagtgatcgt acttcctccg ccccctgatg
    28021 ttccgccaga caagaccatt cggactgctg cacgggcaat ccctttgata tcaaacccgt
    28081 gatgctcgta aaaattccta tcttctgtcg agattactgc atcttggata tagggagaaa
    28141 tcgcgtttaa ttctacatat gttccttttt gggcgtacag tgtgccagct tcttcgtttt
    28201 tcttatcata aatgactgtc gattggctta atcctgattt taacgtttcg acatttgctt
    28261 gttttgctaa aataaagagg tagatacttg tcactaagac acagatcaaa ccaatcagca
    28321 aaatcaattt gttcacatga tattttttcc agatgcgctt tcgccatcga tggaattgtc
    28381 cgagatatgg tctgatccaa cgccaaaaac tgatcaaagc agcttttatc ttttctaaaa
    28441 tgctttgaaa atccatttgc ttcttctcct gtctatttga ttcaatcgtc ttattttacc
    28501 atagtgaaag agtgttttct tcctgcttaa gttgtagttc aatatgtttt tttacgttag
    28561 tgcaagtaga gtaacataag aatgaacaga aaaatgtaag ttttttctta aatatgtttt
    28621 aaaaatggta caattagtct gcgattgcac tgaaaggagt acacttgtga aaatcacgat
    28681 cactttacct atgaaccatc ctgaaacaac aattcgcgac ttgcttgaaa aggaatggtt
    28741 agttcctaga aaggttcgac actttttgcg tatacgtaaa aacgtttgga tcaatcaaca
    28801 acctgctttg ttccatcatg aagtaaaggg caatgaccag atcacattgt tattcgaaga
    28861 aacagattat aattatcaag aagttcaatt aggagatgcg aagcacatcc gtgttttata
    28921 tgaagatgag catttgatta tagtggataa gcctgcagga atgaagactc atccaaatga
    28981 accgacagaa aacagtacct tactcaatca tttagctgct tatctcgaca ctaaacagca
    29041 acgaccttac gttgtccacc gcttggacaa agaaacaagc ggtgccgttt tatttgcaaa
    29101 aaatccattt gtcttgccga tacttggaag aatgttggaa caaaaaatga tctatcggag
    29161 atatcaagcc atcgtatggg gagaaattgc caaagatatg accatcacca ataaaattgg
    29221 acgagatcgc catgatcgga gaaaaagggt cgtagatgat aaaaatggtc agagcgcctt
    29281 gacacatgtc actgtcaaca aagtactttc tgaacaaaca caagtctatt gtgttttgga
    29341 aaccggacgt acacaccaga tcagagttca tttagctcat aagggacatc cgatcgttgg
    29401 agaccctctc taccaaaata gaacagctga acgactgatg ctccatgcat tagaactgca
    29461 catgatccat ccatttactc aagaaaaaat agtcggtaaa gctttacctg gattatggga
    29521 atagaaataa aaaagaacag agtcttatca gcagataatg ataagactct gttctttttg
    29581 tgtaatttgt ctgcttactt ttgatgttcg tgttctgcta acagcaaact accgataatc
    29641 ccactttcat tcggcagtcc ccaacgaacg atatattcac ttacttgagg tgtttccata
    29701 taaccaccca tcaattcaac aaattgctga cggatttttt gaaggagatg atcctgattc
    29761 attaccccac cgcctaaaat aatcttttca ggtgctaacg tcaacgtata gttgatcagt
    29821 gcttgagcaa tataataagc ctgaatagcc caaacaggat gatcttgcgg cagatcttgc
    29881 cctttgatcc ctgtgcgtgc ctcaatagac ggtcctgctg ctaatccttc taaacaatct
    29941 ttatgataag gacaagtacc ttcataatgg tcatctggat ggcgtttcac ccaaatatgt
    30001 cccatttcag gatgtgcgat ccctgaaaaa atttcttcat tgatcacgac acctgcaccg
    30061 attcctgttc ccacggtaag gtaaatacaa ctattttttc cttgtgctgc tccttgcttc
    30121 aattccccat atgcagcagc attgacatcc gttgtccaag cagcaggaat attgtagcgt
    30181 tccttgattg tgccaagaaa atcgaattgt gcccaccctt ttttgggagt tgccaaaacg
    30241 tatccgtaat tatcttttgt tttgtcgata ccaatcgggc cgaatgagcc aatcccgatt
    30301 cccttcaatt cataacgatc aaaaaattca aatacctgat ccaatgtttc ttctggtgtt
    30361 gtggtaggga tacttaattt t
//
LOCUS       DAZKCC010000027        29017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000027 DAZKCC010000000
VERSION     DAZKCC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.027"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..746
                     /locus_tag="ACG8VH_001289"
     CDS             <1..746
                     /locus_tag="ACG8VH_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFP9109856.1"
                     /translation="ELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSG
                     LTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLT
                     PGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVE
                     TQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTG
                     ETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1099..1947)
                     /locus_tag="ACG8VH_001290"
                     /pseudo
     CDS             complement(1099..1947)
                     /locus_tag="ACG8VH_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2100..3212
                     /locus_tag="ACG8VH_001291"
     CDS             2100..3212
                     /locus_tag="ACG8VH_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9109857.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3290..3583)
                     /locus_tag="ACG8VH_001292"
     CDS             complement(3290..3583)
                     /locus_tag="ACG8VH_001292"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP9109858.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3751..4065
                     /locus_tag="ACG8VH_001293"
     CDS             3751..4065
                     /locus_tag="ACG8VH_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP9109859.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4086..6065
                     /locus_tag="ACG8VH_001294"
     CDS             4086..6065
                     /locus_tag="ACG8VH_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9109860.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6078..6404
                     /locus_tag="ACG8VH_001295"
     CDS             6078..6404
                     /locus_tag="ACG8VH_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP9109861.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6420..6938
                     /locus_tag="ACG8VH_001296"
     CDS             6420..6938
                     /locus_tag="ACG8VH_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109862.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6938..8290
                     /gene="celB"
                     /locus_tag="ACG8VH_001297"
     CDS             6938..8290
                     /gene="celB"
                     /locus_tag="ACG8VH_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9109863.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8374..9879)
                     /locus_tag="ACG8VH_001298"
     CDS             complement(8374..9879)
                     /locus_tag="ACG8VH_001298"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP9109864.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9971..10561)
                     /locus_tag="ACG8VH_001299"
     CDS             complement(9971..10561)
                     /locus_tag="ACG8VH_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9109865.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10740..12386
                     /locus_tag="ACG8VH_001300"
     CDS             10740..12386
                     /locus_tag="ACG8VH_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP9109866.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12435..13154
                     /locus_tag="ACG8VH_001301"
     CDS             12435..13154
                     /locus_tag="ACG8VH_001301"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9109867.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13633..14580
                     /locus_tag="ACG8VH_001302"
     CDS             13633..14580
                     /locus_tag="ACG8VH_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109868.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14592..15512
                     /locus_tag="ACG8VH_001303"
     CDS             14592..15512
                     /locus_tag="ACG8VH_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109869.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15553..17010
                     /locus_tag="ACG8VH_001304"
     CDS             15553..17010
                     /locus_tag="ACG8VH_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9109870.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17355..17969
                     /locus_tag="ACG8VH_001305"
     CDS             17355..17969
                     /locus_tag="ACG8VH_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9109871.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17962..19686
                     /locus_tag="ACG8VH_001306"
     CDS             17962..19686
                     /locus_tag="ACG8VH_001306"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9109872.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19683..21134
                     /locus_tag="ACG8VH_001307"
     CDS             19683..21134
                     /locus_tag="ACG8VH_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9109873.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21607..23760)
                     /locus_tag="ACG8VH_001308"
     CDS             complement(21607..23760)
                     /locus_tag="ACG8VH_001308"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP9109874.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23762..24808)
                     /locus_tag="ACG8VH_001309"
     CDS             complement(23762..24808)
                     /locus_tag="ACG8VH_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9109875.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24958..26247
                     /locus_tag="ACG8VH_001310"
     CDS             24958..26247
                     /locus_tag="ACG8VH_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP9109876.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26260..28962
                     /locus_tag="ACG8VH_001311"
     CDS             26260..28962
                     /locus_tag="ACG8VH_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9109877.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9435 a         5016 c         5876 g         8690 t
ORIGIN      
        1 tagaattgac gaaagcaaac cgacttacac caggcggtgt cgtgttgaca aagatcgatg
       61 accaaagcgg ggaagtcctg caaggagctg tttttgagtt acaagatgcc aatggaaccg
      121 ttctgcagtc aggtctaacc acaggagcag atggaaaact ggcgattgac ggcttagcac
      181 caggagtgta tcagctggta gaaacccaag cgccaacggg gtatgaattg gatgcgacac
      241 caatcgagtt tgagatcgaa agaagtcaga cagcggtggt agaattgacg aaagaaaatc
      301 gacttacacc aggcggtgtc gtgttgacaa agatcgatga ccaaagcgga gaagtcctgc
      361 aaggagctgt ttttgagtta caagatgcca atggaaccgt tctgcagtca ggtctaacca
      421 caggagcaga tggaaaactg gcgatagatg gcttagcacc aggagcatat cagctggtag
      481 aaactcaagc gccaacgggg tatgaattgg atgcgacacc agtaactttc aagatcgaaa
      541 aagaacaaga agtggcgatc tttttaacaa aagagaatag aaaagttgct gatagctcag
      601 atataagagg aaacaatcca acaagcagtg gaaataagca tcttccaaaa actggagaaa
      661 ccatcattag ccagtttata ttgtcaatat tgggtgtgct acttgttttc atcagtataa
      721 aatttagaaa gaaacgaaat atatgagcta aaataaaaaa ttgaataagc atagttttta
      781 aaatcaacta gtacccatca attgaatgga aactaccgat tttagaaggg gtatacaaac
      841 aaatattagt atcggagtag agaaggttga acccaaccag taaataagta aagacaattt
      901 tgtgctgcgt tgagaagtga aaaataaacc tttgtagtaa tactagttaa taaaaaatat
      961 acaaaaacac tgcgaaaatc attcttgagt ttcgcagtgt tttttgccta atgactcaag
     1021 tttattgggt aatactttga ttttctttct acaaataatc agcgatcagt tgtaattttt
     1081 tctctagttt taatggctct ttgtcaataa ggagaaaaac aagtcgggtc ttttgtctcg
     1141 gaaagcttca accaactcgt gaataaaggt tacgcctcct tgaaaggcgt ggaaaaacca
     1201 agtaatcgat caatcaccaa tttcgcattt ggtagaacat gtttggtgag ctgaaagtag
     1261 gtggcgttca tgtctgtaac taagaattct atttctttag gattagtgca gctaaggaaa
     1321 tagtttgtta atcgaggaat tttttgtgta ggtaaatttt ctcgattaat ttttctgtct
     1381 cgccatccgc ggaaataaaa ctcagtttat cctcaacgga aacgtgagaa cgaaattcac
     1441 aaccctcaac actcaagaca gaattctttt ggaagacttt ggtaaatagc ttctcaactc
     1501 tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa cttttcgaaa taaaaaataa
     1561 cgacactttt tcagtcagta aagaagtgat tttatgtctg acatgatttg aaatcgagtg
     1621 ccttggacgg ataaaataac tttgagcgtt ccaatggctt cgacagtttt tacaggtata
     1681 gcgctgcttt tttaggcgta taaccattgg catatgatta tattattcaa agtggacaat
     1741 ggtttccttc ttcttgtttt tcaccaccat taatttccta tttccatcta ccacagcaga
     1801 accacaattt ctacagacac atggagcagg gaagagaact gcagcaacga tcaatgtttt
     1861 tttcttctgt aaggtttcgt aagagacctc tgtaatcatc aaatttttgt ctattgatct
     1921 cagaattttt ttgatagaac cattcataaa gcatatcgtt ctctcagttg tttattttgt
     1981 ggtgatttaa tcatacaaga aaacgatatg tttttttata aaaacatgaa taggaaaaat
     2041 agttgacaag aaaatgacaa cgccatatga tgttccatag tatttacaac ctgtcataga
     2101 tgactgtaaa aaataaaagt aaaaagaaag gacgacaaca ggtggatttt tatcagtctc
     2161 tacaactaga tccgtttatt ttaaaacaaa aaatacgaga agcagcttct aaaaaagaaa
     2221 agtgcatgta tatttgttca ttgtttttac gatcattatt catcgtattg tttgccattt
     2281 gttttatcat tataattaca acattatttg aatcgaagca taaaccatac gctgtggttt
     2341 tattctgtat gttgatgagt attcgttttg ttgactttgg ttataagatt tcccattcga
     2401 tcataggttt agcaatcgtc atgttctcac tattagtagc accgtatgtc cagctgatca
     2461 aatggtcagt tatgggcatg ttgattcatt ttattttact aagcagcata ttaatggcaa
     2521 ccgctagtga tcccaaaatg gggaatgctt ctttgtacgg cttttcatat ctctttattg
     2581 tttattcgtt acctaaagat tcgttgaaca agaatttctt tacacaaaca agtagcttat
     2641 tatttttgtt tttttgttgg ttttcagttc tcctttacag aaaacatcga gaaaagaaca
     2701 aagacaaaag cttgtttaaa gagatttttt tgaaaggcat gtattcgcag gaaaaaattt
     2761 ggatgctcat ctatgcattt ggcattagtc ttttgatcgt tgcaggagag tatgtccctt
     2821 ttcagcgatt gatgtgggca gggtttgcat tttcttctat tgtatctagc tatggattga
     2881 tgtcgattgg tttcaaagaa cgagcagtag accgaatcat tggctcattg attggatgtg
     2941 ttttatttat tggtatcagc caatttattc cttttaactg ggtcggtata ttaggtggtc
     3001 tagctttagg tgtttgttct acctatagat ataaaacagt ttttaattct tttggtgctt
     3061 tggcaatcac tgccagttta ttcggggttc caggagcagt aacgatccgc atttttgaga
     3121 atatccttgg ggtatgttta ggaatcatgt atattggggt gactgaaata cttatccgaa
     3181 aaataagaga aaaacacggt ttgaatcatt agatgagatc gaataaaaaa taaggaaaaa
     3241 gtgaagatac acatggatga taaattgcta tcattccatg tgtatctttt tacggtaaat
     3301 cagtgaaacg ttcgactttc atttttaatt tatacttttt acggagttcg tttatttgag
     3361 ccatcatttc ggatttgtga tgcttttcga ttgcttcttc atctttccaa cggtcgatca
     3421 gtaaaagaga ttctggatct tctagtggaa agtaatagtc atatttctcg tttccttctt
     3481 ctgcacgaat cgctttgaca atgtttcttt cgaccatttc ttctgcaaat tttctggctt
     3541 gtccatttgt acttgtatac aaaatattca ctgtgatact cactaaatcc cctcctgaaa
     3601 aaattctatc actagtatat cacggtgcca gaaattatta ttcaagatat gatacacatt
     3661 ttttccgcaa tgctacggaa atatatattt tgaatagaaa acgcttacaa gatatgcttg
     3721 gtttataaaa ataaaaggag tgattgattt atgaaaaaag cattgattat ttgtgcggca
     3781 ggaatgtcat cctcgatgat ggcctcaaag acaacggatt tctttaagca aaaaagagaa
     3841 gaggtactcg tagatgcagt ttcagctaca gaaggcgata atatgattaa gaatagtgat
     3901 tttgatttat ttctgatcag cccgcaaacg acgatgtttt tggataaatt caaaaaatta
     3961 ggagaatctg ttggaaagcc tgttgttagt atccctttcc aagcgtatgt cccaatccct
     4021 acaggcatcc agaagttagc agaggtgatc gaagagaaca tatgatggaa taggagtgga
     4081 tgataatgaa acagaaggaa aaattgctgt tgagatattt gattgatcac aagaaggagt
     4141 atgtcaccag tcaacgttta gcttctgaat tgttattatc tgatcggaca atccgtaatt
     4201 acttgcagcg gatcaaagag ttagtagaaa aaaacggagg taagatcatt gctaaaccag
     4261 gttatggcta ccaactgcat attttacaaa gactgacatt tgacttgttt ttgagtcggc
     4321 aagaaatagt acctggatat gctagagaac cgcaaggatt ttatgagtcc gaagatcgac
     4381 aaaagtatat attgaaccaa ttgcttttgg aagatgccat tctttttatg gatgatttgg
     4441 cagaagagct ttatatcagt cgttccagtt tgacaaagga catgcaagaa atcaaggagc
     4501 gattgattcc atattcacta aaaatcgttt ctaagcatgg tcaagggaca tggatcgaag
     4561 gggaagaaag aaatcgacgg cattttatca tggatacttt ttttggaaac cattatggaa
     4621 attcattgaa agaatattta gggcaaagcc gttttttccc agacatcagt tttgaagagc
     4681 tagtcattat cattctcgat gaaacaagag aagctaagtt aaaagtttcg gattttatca
     4741 tacagaatct gactttacac ttggctttgg ggatcaaacg gctccgagaa gggtttgaaa
     4801 tcaaggaatt aggcatcgaa caagaagttt ctaagcgagt agagtatcaa gtggcgcagc
     4861 ggatcgttca acgtattgag gtaatcgcag gtgtatcgtt tcccgaagaa gaaattgctt
     4921 atttgacatt acatctcatg gctaagtcga atcacaaaga aaatcaaaat gatcaggaac
     4981 tatcaaagga attagcgaaa gttctgaaaa gattgtctca ggaatttcat ttttcattag
     5041 tagaggatct ccagcttttc aatggactac tcgatcattt gtcaccgatg atgattcgat
     5101 tgagccgagg tattcgcatt gagaacccat tgactgagga gataaagaaa gaaaatccca
     5161 aagtctttga cgcagtaaaa aggcatttca gcaacatgcc ggctttgaaa aactatacca
     5221 tcaatgaaga tgaatgggcc tatttggctt tgcatctcat ggcggcattg gaaaaagaaa
     5281 gagctgcgca taaactacat gcattgatta tctgtgcaac gggttacggc agtgcccaac
     5341 tattaaagaa tcgcgtagtc agtgaattcg gtaaaaatat tacagtggta agtgtaaaag
     5401 gctattatga gatcaatgaa acaactttaa aaggaatcga cctgattatt tcttctattg
     5461 atctttcgac aatgtttttc aaaattccag ttttacatgt cagtattttt ttgaatgaag
     5521 atgatgttcg taaaatacga aaggtagttg gagaatctat accctatcat tcctcagata
     5581 gaccgatacc agcattttct ctacagcaaa aaaagcagat ttacaccgaa cagttgtctg
     5641 aaaaattttt caagacttat acgtacgaac cgaaaaagga agaaattctt catgatttgc
     5701 tgcaaaagct gagcataaat gaagaagaag gatatataag agaaatgcga aagcagatcc
     5761 agcaacgtga aaatatggga caaatcatat tcagtgatac agtggcagtc ccacatcccg
     5821 ctgttccagt tggaatcgaa acaaaggtag ccgttgcatt gattccttct gggatgcagt
     5881 gggatcaata tcaagaaatt cattttgttt ttttagtttc gccttcttat gtcgaaaatg
     5941 aaggaattac ggtcgtgact aaagcaatcg ttaggttagt tgaccagcta aaagtacagc
     6001 aggaaatctt agctgaacct atttttgaga atttcagcag taaatttatc aaactgatcg
     6061 aataaggagg aaaacagatg gctgaaaaaa taaacagtga aacactacaa gtgacttctt
     6121 ttgatatcat tttccatagc ggaaatgcac ggacactgat acatgaagca tttcgctcga
     6181 tgcgtaaaga gatgtttgaa gaagtagaag agaagttgcg acaagcaaat gaagagattc
     6241 ttcatgcgca tcagtcacaa actcaacttc tgaaagaata tgccagcggg acaaaaatag
     6301 aaatggaaat tatcatggtc catgcacaag atcatctgat gacgacgatg acattattgg
     6361 aagttgcaca ggaaatgaat tatttgtatc aaaaaaataa atagaaagga aggatcattg
     6421 tgcaggatac agtaaaaagg aaaatcgaat tagaaaaaaa gcaacttagt ttgaaaaaca
     6481 tgtatttcaa tcgttatcta atcgtacgtt acctgactgc ctttttcttt ttcatgaaca
     6541 tgcaatggat gattctgctt ctatcagcta aaagtttagg aagtagttta ccgatgattc
     6601 tgattttagc tattttgcct gcagtaggag aacaagtaaa gctttatcga aaacaccaaa
     6661 cgaacgttcc atggacaaag tggtattttt tgttccagag tgtctttaat ttattagtga
     6721 ttccagtgtt gtttacctca ggatttacat tcttgtatcc ttttatggca aataacaatg
     6781 gaggacagtt atttgttttc attcttgttg taagcggtat ttttgccagt gcatttatac
     6841 agtatcgttt aaagaaaatt tcactcaacc aagaccaaca gtatatccgt attaaacaat
     6901 atgaaaaagc gttatattta ggaaaggaga ataactaatg tttgattttt tgcagaaata
     6961 cctgatggga cctatgggga aaattgcgca acttaaaatc gttcgggctg tcatggcagc
     7021 ggggatggct tctattcctt tcacgattgt aggctcgatg tttttggttt taaatatttt
     7081 gccattaccg ttcccatttt tagaaggatt ttttaatgca acttttttca aagttagtga
     7141 cttgtacatg atcgtcaata caatgacgat ggggatttta tctatttact ttgccattgt
     7201 gtttgcttat gaattaacgt caattgaacg agatgaacaa aaattaaatg ttaatccgct
     7261 cacgggggca ttactatctg tttttgcatt ctttatgtgt atccctgaat tgatcatgac
     7321 tgatggtaaa atcagcttga ttgcttctat caatgagaca gaaactgtag taaatggtat
     7381 taggatgggc tcttttgtcg aacgtttagg aacttcagga atctttacag ctattattat
     7441 gtcgattatt gcggtcgaat tgtattgtat gtgtgtcaag agaaattggg tcataaaaat
     7501 gccagatgtt gttccgccag gagtttctcg ttctttcact gctctgatcc caacatttgt
     7561 cattgcgttt gtggtgatga tcatcaatgg attattagtt gcactaggaa cggatatctt
     7621 caaaatgatt gctatcccat ttgggtttgt tacgcagttg acgaatacat ggcttggtgt
     7681 catggtgatt tatttcttga tccatgcatt gtggatcgta gggatccatg gagcgaatat
     7741 catcacttct ttcttgaccc cgatcgtatt agctaatatg gcagctaacg cacaaggcgc
     7801 aaattatccg ttggcaggag aattcaacaa ttcttatgta accgttggtg gatcaggtgc
     7861 gactttagga ttgatcatct ttattgcatt cctcgcgaaa tctgatcagc tgaaagtgtt
     7921 aggaaaagcc tcacttgtac caggattatt caatatcaat gaaccaatca ttttcgggat
     7981 gccaatcgtc tacaatccct atttagcggt tccgtttttc ttagctccga tggcttctgc
     8041 tacactcgct tattttgcca ttcggctgga aatcgtcaaa ccgatgcttg cacagatgcc
     8101 gtggccttca ccagtcggga tcggagcatt tgttggtagt ggtggtgatt ggaaagctgc
     8161 tgtgttagca gtcatttgtg caatcatcgc attcattatc tggttcccat ttatcaaatt
     8221 ctatgataat aaattattaa atgaagaaag agaaaaggca gcagaattag ctagtgaaaa
     8281 gttaaaatga tttctaagaa aaaaggaact gtgacaaaaa atcacagtcc ctttttttct
     8341 tagaaatggg aaaccactag tatataaata ccattattct tcaatcaacc gcttcacgat
     8401 tgcataatcc ccttcataag gttcatctac accattgatt ttttgatagg gatccacgcc
     8461 ttttcccgca ataacgactg aatcttgcgg agcgctttct gctaaagcgc gtcgaatcgc
     8521 ttcaggacga tccatttcat aatgaacgat caaatctgga ttagtaattg cttcattgat
     8581 ttcttccgcg atttttttcg gatcttcaaa tgctgggtca tctgccgtca aaaaggcgat
     8641 gtcagctgtt tctgatagca cttttccaaa atcatgtcga cgggagattg ctttgtttcc
     8701 tgggctgcct aatacaacaa ttacacggcc atctggatgt tcatttttgg caaatttcag
     8761 caatgttttc aagcttagat aattatgtgc atagtccaca tagacatgcg ctccattgga
     8821 ttggatcaat tgatccattc ggcctggtac acgagcttct tttaatccgt cttgtgcatc
     8881 ttgactggag gcacctgtta gtgctgcggc aatgatcgca ctggctgcat tttcttggtt
     8941 gaatcctcca gccagcaaaa tatcataagc agtatcgcag atagctaagc gatcttcatg
     9001 agaagataaa gtaaaaccgt gagtaccttt atctgaacgg atcacttgat aattgctttc
     9061 ttccgaacga ccataagtga tcgttggaat ctggaataga tcggctgttt ccctaagtaa
     9121 atcaaaatag tcactttcat gatttaaaac aactgttttt gaattcaaga tcaattgtcg
     9181 cttgcaatag aaatagtcat caaatgtcgg atgttcgatc ggactgatat ggtcaggcga
     9241 gatattcaag aagattccga catctaaagt caagccgtat acgcgttgtg ttttgtaagc
     9301 ttgtgaagaa acttccatca ctagatgagt cataccattt tcaactgctt ctgacatcat
     9361 acgatataaa tctaatgact caggcgttgt caaatgggat ttgaaatatg ttttgccatc
     9421 taacgttgta ttcatagtag aaagcaatgc tgttttttta tttgttgtgt gatccaagat
     9481 tgcttttgta aagtacgcag cagttgtttt accttttgtt ccagtgaatc cgatcacttt
     9541 caatttattt tgcggatagt cgtaaaaagc catagcaaga agcgccattg ctttacggat
     9601 atccgttacg atgattccta ctgctgaatt ttcgtatggt tgttctgata cataatattg
     9661 aataccctgt tcgattgctg aatccaaata ttcttctttg aaattcattc ctttacagaa
     9721 aaacaaagta gaagcatcag cttttcttga atcataactt aactgaagaa attcagcttt
     9781 ttctggtaca tcaagatgcc agcctcttgt tgaaacaaat tcttttaata gattttcttt
     9841 taatagtaaa tgacggatgt cttctaaaga gaagctcatt gattgatccc tcgctttatt
     9901 caatttcttg gtttattata gcacactctt tttaatatat cgtttgtttc cgctatgaca
     9961 aaatccagct ttattcaaat tttcttgaca caggggacaa gcggatgagt attttcataa
    10021 acaatgaact gactgtaata gataggagaa cgaatagtgt ctccaacaat ccaaaaacat
    10081 aataattcaa aaagacgata agactattgg taagtaaaag tccttgcggc attggaagcc
    10141 gaaaaaattt gtggaaaata atcggcggta tcgttatacc gccatttgag gccccgactg
    10201 tgtacaatgt gtaaacccca agactgaaca aaagacttcc tgaaatcagt gaaaagaaat
    10261 gtgaggagat catttccact tttggaacaa tctctaaaac aacaggaaaa agcagacttc
    10321 ccgctacagt cttaaaaaat actgattttc ctaataggac agcggctaga agcagcatca
    10381 taagattgat gctccaaaca gtaactgagc gttggataga aaaggccgtt tcaactaaat
    10441 gtccgatggc gcccactccg gcggaagcta tatcgtgagg gagcaagaac caattcaatc
    10501 caactgtgat aaagaaaaga cctaaaaata taacgggtag tttttttatt tcttgtgtca
    10561 ttgatatctc ctataaatgt tagccataaa aggctgtata tttctttact ttaccaaaaa
    10621 aacgaaaccg aattcaaaaa aacaattgtt cttataaaag ttatataaaa tcgattgcca
    10681 gtttctgttt taccaattaa aatgatagta atgttttgta gaaagttgga atctgaccga
    10741 tggataatca aagaaaacca gcattagatc aattaacacc gcaagaaaaa atggcacgag
    10801 gctctgcttg gatgaccgcc agtaatatca tttcaagatt gctaggcgct gtctatatca
    10861 ttccctggta tgcttggatg ggcgaaaatg ctaaggctgc caatggatta ttcaatatgg
    10921 gatacaatat atatgcattg tttctactga tctctacagc aggaatacct gctgcgattg
    10981 caaaacaaac tgcacgctat aattcattga atgaatatgg cacttcacga cgattattta
    11041 tccgtgcatt acagatgatg ggcgggttag gcctgttgtt tgctttgttc atgtacatcg
    11101 cctctccttg gctagctcat gcttctggtg gcggagaaga actgattcca acgatgcgtt
    11161 ctttgagtat cgctgtattg gtttttcctt gtatgagtgt gatacgaggg tatttgcaag
    11221 gcaatcaaga aatgatgcct tatgcgctgt cgcagattgt agaacaggtg gcacgtattt
    11281 tttatatgct attggctaca ttcatcatca tgaaagtcat ggatggtgac tatgtgacgg
    11341 cggtcacgca atcaactttc gcggcattta tcggtatgct agcaagtatt cttgtccttt
    11401 tgtatttttt gaagaaacat caagcgtata catcggcgtt tattcactac agcgaaaaca
    11461 aaataaatat tgcaacgaag gaattgttgt tagataccgt taaagaagcg attccgttta
    11521 ttattgtggg ttcaggggta acgatcttca aattagtcga tcagttcact tttatgaaga
    11581 tcatgtctgc atccaccgag tattccaatg cacagttact ggatcttttt tcgattttta
    11641 gtgccaatcc agataagttg acgatggtag ttattgcatt ggcaacatcg atcgcgtcta
    11701 ctgggttacc gttgattaca gaagctgtta cgttgaaaga tcgagtaggt ctggcaaaat
    11761 tgacaagcgg aaatttacag ttgttttcat tttttatgtt tccggcgaca tttggtgtta
    11821 tgttacttgc ctatccgcta aatacattat tttatacacc ggattctcta ggaagtaatg
    11881 tactgatcca agcaagtttt gtaggactct tcctaggact atatatgcta gtttccaaca
    11941 tgctgcaagg aatgtttgaa aacaaggcgg caattaatta tttggttatt gggtttctag
    12001 tgaaactcct tcttcaatac ccagccattc gtttatttga agtatacggc cctttattgg
    12061 cgacaatgat cggatttgct gtgtcttgta ccttgatctt aaagagaatc catgaagtag
    12121 cccgctttaa accaagtttt gtctggcgca ggacattgct gatctttatt cttaccttga
    12181 ttatgctttt ggcttcgtgg atcacaaaga tgatttttgg tgctttttta aacgaagata
    12241 gcaaattcca gtctctattg ttgattcttg tagtagcagg tataggggct ttagtgtatg
    12301 gttatttagc tttgaaaatc cgcttggcag aaagactact aggaccagga atagcgcgtt
    12361 tacgacataa attaaaaata aaataagtga atcagaagag ctagattgat gctcttttga
    12421 aatgggggaa acgcatgcgt ttagataagt ttttagcaga tatgggactt ggcagccgca
    12481 aagaagtcaa aggattaatc aaaaaaggaa cgatcgaagt caatggacaa gtggtgaaaa
    12541 gcgataaagc gcaagtaaat gaattcgaag atgcggttac ttatttagga gaaatcatta
    12601 cgtatcaaaa agatttttac tacattttgc ataaacctgc aggagtcatt tctgctactc
    12661 aggataatca tgatcaaacc gtaatcgatt tattggaaga ccaggattat cgggaagatc
    12721 tttttccagt cggccgatta gataaagaca cagagggcct cttgatttta acgaatgacg
    12781 gggtgtttgc ccatcagtta ctatcaccta aaaaacatgt ggagaaagaa tatctagctg
    12841 aggttagcgg tataatgaca gaagaagata tccgtttatt tgcagaagga ctgattattg
    12901 atggcgatga gcagacattg ccggcaaaac tccaaattga tcaagtagat caagagacag
    12961 aaacttcaat tatccgtctg attttgcatg aaggaaagtt tcaccaagtt aagcgaatgg
    13021 tcaaagcagt aggaaaagaa gtcttatatt taaaaagaat ccgtatggga aaattttggc
    13081 tgcctaaaga tttgaataaa gggcaatatc gacccatgac caaagaagag ctagaacaag
    13141 tgagagaaaa ataattgaat ggatagctaa aagaataagc agagtagttg tagcgccatc
    13201 tgaaaagcag acttgtccgt ctgactttcg gatggctttt ttgtgaaatt tcggcttatt
    13261 tccgtagaag ctacttctgg aacaccgttt gttcggaata agggactgtg acaagacttt
    13321 tgttacagtc cccattgttc cttgtgactc agagttagat agtaaggaca cgatctccct
    13381 aatactcagg ccaaaacgct gaattcacaa cctctgataa acggtgttca atcagctgac
    13441 cctcggtatt agctcaaaaa acggcaaaat atgaggaact attttcccgt ttttttctct
    13501 aatactcagg ccaaaacgct gatttcacaa cctctcctat actttgtttc attttcccta
    13561 tactttgttt atttattctc attattgaaa gcgctataat tacgttgcga atgaatcgaa
    13621 aggggttttt agatgaaaaa acgaaagggg ttcaatcatt tttttatgaa tctatggcgt
    13681 tataaagcac ttgtcctgat ggctgtaccg ggtatgcttt ggatgatttt tttcttttac
    13741 atacctgtaa tggcgaatgt cgtagcattc aaaaattttc atatttcagc agatgggttc
    13801 ttggctagct tgagggaaag tccttgggtg ggttgggaaa atttcaaatt tttattttct
    13861 tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc atttatcctt
    13921 ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag aaataaacga
    13981 ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg ggtcattatc
    14041 agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca gtggatcact
    14101 ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc gtttatcttg
    14161 gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt tgcatctgtt
    14221 atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag caaatggcag
    14281 cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt aacgatttta
    14341 gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc taggaattcc
    14401 ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg cctgacatcc
    14461 acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt cggctttgtc
    14521 ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc attattctaa
    14581 ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct ttcaataaga
    14641 cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt attttgccat
    14701 ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact cacgggtaca
    14761 gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac cagatgcagg
    14821 ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt acactgatca
    14881 atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt ccattccgtc
    14941 gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg gtggccaatt
    15001 atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg atcttgccaa
    15061 tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt tcggtctctg
    15121 attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata tttgttcaga
    15181 tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc gcattgggtt
    15241 actggaatga ctggttcaat gctttactat acatccaaga tgataagcta gttccactac
    15301 agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat tcaggagcag
    15361 gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga atggcgatcg
    15421 tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag cattttgtca
    15481 aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat cataaaatta
    15541 ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc agcgttgttg
    15601 ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga agctagcaat
    15661 tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga tcaattgatg
    15721 gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat caattacatt
    15781 ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga aaattatgat
    15841 atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc tgatttgact
    15901 gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc atatattaaa
    15961 ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt ctttgctcaa
    16021 caagtactta cttttaacaa agctttacta gataaatacg atctttcaat tgatggaatc
    16081 caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga accaaacacg
    16141 gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta tcctttaggg
    16201 aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat caaccaatac
    16261 gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa acaaggactc
    16321 attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa tacttggcta
    16381 atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac aaatgcagca
    16441 aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc tcaagcacaa
    16501 atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt agaacttcta
    16561 gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat cgagggagaa
    16621 gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg ttatcagcca
    16681 aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac acaagagtct
    16741 attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga aacttcaccg
    16801 attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa tatttctaat
    16861 gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga tgaaacatta
    16921 ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa agaaatgcaa
    16981 aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg gttgtgacaa
    17041 aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt ttctcggtat
    17101 taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc ttactactcg
    17161 aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct aaccctcggt
    17221 attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc tctaataatc
    17281 aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata aacttaatgt
    17341 ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg atctgggtca
    17401 ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc atctttggat
    17461 taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc gagtataaag
    17521 aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga ggcaatctgc
    17581 tatttagttt atttttatct ggtactgctt tattgagtta caatttatat ttatctgttc
    17641 aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc ttgctctgcc
    17701 tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc tctgttccta
    17761 atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt ctaaaaataa
    17821 ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg atcctgtttg
    17881 gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg cgtgaagaat
    17941 tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa aggaacctta
    18001 ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat gactgctacc
    18061 gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa catgacaagt
    18121 gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag taggatcatc
    18181 aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta cttaacaaaa
    18241 cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga tgaattagtt
    18301 tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag tgcgatatat
    18361 atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa aggtggggaa
    18421 ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc tatcacacaa
    18481 ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact agatatgatt
    18541 ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg aacgacaaat
    18601 cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag acaagaaatg
    18661 atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct ggaaaaaaac
    18721 aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt actagataaa
    18781 aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg tatcattatt
    18841 ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc acagcaagta
    18901 tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac gcggatcgac
    18961 ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga aatgttggat
    19021 agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca agacgcacat
    19081 atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt agaatatatc
    19141 cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta tgcgttttcc
    19201 gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga agaactggac
    19261 ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt agcctatcat
    19321 tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat ccagccatta
    19381 gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc gctaagtgtc
    19441 aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg aaaagggatg
    19501 actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt ggaacttcac
    19561 ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg cagcagttat
    19621 tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac attcaaggag
    19681 ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg aatgggttaa
    19741 aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca gagaatgcca
    19801 aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg gatgtgacaa
    19861 tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt cataattttg
    19921 aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt atgcaattag
    19981 gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa agcctcaaga
    20041 aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa atctatcaag
    20101 aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa gaagaaataa
    20161 taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca agaactcatg
    20221 atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat caacgaaatt
    20281 acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat ggcttctgtt
    20341 gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg gaagagacag
    20401 attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta cagttgcatc
    20461 aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa acaaaagatc
    20521 aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaatta gaagtcgctc
    20581 aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg ccggaagata
    20641 tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt gatctggaag
    20701 atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg caagaacttc
    20761 agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac agtgacaatg
    20821 tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca ctcaaagaag
    20881 tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa aaagaaacga
    20941 aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa caactgcttt
    21001 tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac aacaccacgt
    21061 atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac cgagaaaaca
    21121 gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa attgtctaga
    21181 aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat gacctctcga
    21241 taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtggaa
    21301 agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc acgacctctc
    21361 cactaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg caaaatatga
    21421 ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt tcacaacctc
    21481 tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa acgaaaaata
    21541 tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct ttttcacaac
    21601 ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat atttcttagg
    21661 gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat cttgatgggt
    21721 aaaatataat ttcgtatatt ctttgtccat ccgtttgatt ttatggataa attgttgctg
    21781 cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa gcaaaacagc
    21841 cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga caggataaaa
    21901 tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat ctccagggta
    21961 acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg gctgtgccat
    22021 ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct ggtttgaaat
    22081 agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct caaaaccata
    22141 tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt ctttatatct
    22201 ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag caaagctgct
    22261 ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa atggttctct
    22321 gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa agatattttg
    22381 atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta gtaccttggc
    22441 aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga ctgattcgtg
    22501 gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc gtccataatt
    22561 tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt caggcatcaa
    22621 gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca atgtcccagg
    22681 cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt ctctatagct
    22741 gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag aataaagagg
    22801 ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac caggttttat
    22861 agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt cataaaatgt
    22921 ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt gttgttgatc
    22981 atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac tgtgagcaag
    23041 gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt gttccgagct
    23101 gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat acaacgggat
    23161 cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat attcaaatct
    23221 caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg tgatttttcc
    23281 ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg ataaaagtaa
    23341 accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc taggtatcaa
    23401 tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc aaaatatact
    23461 ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta gttttccatt
    23521 gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt gatgggtaag
    23581 acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt tttgatccgt
    23641 agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct ggcagttccc
    23701 attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac cgatattcat
    23761 tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta ctatcggatg
    23821 gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta cctaactgtt
    23881 caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct tggatatcat
    23941 caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc aagagattca
    24001 tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca tacttagtga
    24061 tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg tggaaagtca
    24121 aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg gattgtggat
    24181 gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc aaataagagg
    24241 cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt aactcataga
    24301 tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa agtaagtcaa
    24361 gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg attagctctt
    24421 ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc tgtttatctt
    24481 gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg acatatggaa
    24541 gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc ggagaagcgg
    24601 atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc tctaattcag
    24661 tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt tctttttcag
    24721 ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc aaatcattaa
    24781 atattgtctg gtacaatggt tgattcatat aaacacctct taatgatata tcatattcaa
    24841 ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc gtagacataa
    24901 ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt tttattagtg
    24961 gcttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac acaaaaatgt
    25021 ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa tactctgttg
    25081 acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga cattccagcg
    25141 atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc aaaagaagat
    25201 gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta tatcaatatc
    25261 gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca aacagatcat
    25321 acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt atgctaccca
    25381 atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt caatcaatct
    25441 tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga tcatgaacac
    25501 tctccttata catttgtacg agacacgaca agaaaagaag atacgctgat caatgacggg
    25561 cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc tagcgatgat
    25621 acttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt gttaggttat
    25681 gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca aacacagaaa
    25741 ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa tcaaaaaggg
    25801 caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat ggatgacagt
    25861 aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca agaggaagat
    25921 tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta tgaaggaaag
    25981 tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc gattgcatta
    26041 gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt gaatttactt
    26101 gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa caatcctgaa
    26161 aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt agtcatggat
    26221 tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa agtatatatc
    26281 atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca tcacatgcga
    26341 ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc cttcaacagt
    26401 tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag gccagaaaaa
    26461 agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc attttatatc
    26521 ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct gattggcatg
    26581 gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga tacatttgga
    26641 aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc agcgtttggc
    26701 cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa ctattcttcc
    26761 caatactcag agatgtggtg gaaaggacct gatcaaacag caatctttgg tctgctattt
    26821 gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc tcttgccttt
    26881 tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt attgatgatg
    26941 aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca tctcgcaaat
    27001 gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct ggaagcagtt
    27061 caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag tcaagaaacg
    27121 gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa acaatggaat
    27181 accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat ggcttatgaa
    27241 gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt aatgcaaaat
    27301 catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga aatgatacct
    27361 cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt agaacagctg
    27421 accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt cattgtaaat
    27481 actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga aagaaaaaga
    27541 ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc taaacgtaag
    27601 tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga agaaactgtc
    27661 caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc aagaatgatc
    27721 aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt cgcgcttgta
    27781 gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg acgaatcatc
    27841 gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat ggaagatcgc
    27901 aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga tataggaaat
    27961 gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa caaagaaaac
    28021 tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat tctacaggaa
    28081 atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat ggttgtggaa
    28141 tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat aaaaacaatc
    28201 atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga caatcagatg
    28261 aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca tcacgaagca
    28321 gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg ggaaaacccc
    28381 acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta cggtgtaaca
    28441 gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat cgcagtgacg
    28501 ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc agaagcacag
    28561 tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga agaaaaattc
    28621 tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca aatcaaacat
    28681 catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga aacgtttgca
    28741 attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg tttcaatatg
    28801 tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt catattgaat
    28861 ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta tgagattcgg
    28921 acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc ggaacaaaat
    28981 ttgtgtgtgc ggtttcagat ggtggagaaa ttttaga
//
LOCUS       DAZKCC010000028        28593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000028 DAZKCC010000000
VERSION     DAZKCC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.028"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..1867
                     /locus_tag="ACG8VH_001312"
     CDS             227..1867
                     /locus_tag="ACG8VH_001312"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP9109878.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2021..3343
                     /locus_tag="ACG8VH_001313"
     CDS             2021..3343
                     /locus_tag="ACG8VH_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP9109879.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3405..3836)
                     /locus_tag="ACG8VH_001314"
     CDS             complement(3405..3836)
                     /locus_tag="ACG8VH_001314"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109880.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3900..4736)
                     /locus_tag="ACG8VH_001315"
     CDS             complement(3900..4736)
                     /locus_tag="ACG8VH_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9109881.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4926..5228)
                     /locus_tag="ACG8VH_001316"
     CDS             complement(4926..5228)
                     /locus_tag="ACG8VH_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP9109882.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5254..5685)
                     /gene="ruvX"
                     /locus_tag="ACG8VH_001317"
     CDS             complement(5254..5685)
                     /gene="ruvX"
                     /locus_tag="ACG8VH_001317"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9109883.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5682..5948)
                     /locus_tag="ACG8VH_001318"
     CDS             complement(5682..5948)
                     /locus_tag="ACG8VH_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP9109884.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6202..6927)
                     /locus_tag="ACG8VH_001319"
     CDS             complement(6202..6927)
                     /locus_tag="ACG8VH_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9109885.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7293..7363)
                     /locus_tag="ACG8VH_001320"
     tRNA            complement(7293..7363)
                     /locus_tag="ACG8VH_001320"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7329..7331),aa:Gly,seq:ccc)
     gene            7480..8232
                     /locus_tag="ACG8VH_001321"
     CDS             7480..8232
                     /locus_tag="ACG8VH_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9109886.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8248..8727
                     /locus_tag="ACG8VH_001322"
     CDS             8248..8727
                     /locus_tag="ACG8VH_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9109887.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8978..10558)
                     /locus_tag="ACG8VH_001323"
     CDS             complement(8978..10558)
                     /locus_tag="ACG8VH_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP9109888.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10551..11294)
                     /locus_tag="ACG8VH_001324"
     CDS             complement(10551..11294)
                     /locus_tag="ACG8VH_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109889.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11470..12246)
                     /locus_tag="ACG8VH_001325"
     CDS             complement(11470..12246)
                     /locus_tag="ACG8VH_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9109890.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12494..13330)
                     /locus_tag="ACG8VH_001326"
     CDS             complement(12494..13330)
                     /locus_tag="ACG8VH_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP9109891.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13341..14270)
                     /locus_tag="ACG8VH_001327"
     CDS             complement(13341..14270)
                     /locus_tag="ACG8VH_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9109892.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14462..14815
                     /locus_tag="ACG8VH_001328"
     CDS             14462..14815
                     /locus_tag="ACG8VH_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9109893.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14889..15545)
                     /locus_tag="ACG8VH_001329"
     CDS             complement(14889..15545)
                     /locus_tag="ACG8VH_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9109894.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15771..17705
                     /locus_tag="ACG8VH_001330"
     CDS             15771..17705
                     /locus_tag="ACG8VH_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9109895.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17791..19014)
                     /locus_tag="ACG8VH_001331"
     CDS             complement(17791..19014)
                     /locus_tag="ACG8VH_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9109896.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19008..20879)
                     /locus_tag="ACG8VH_001332"
     CDS             complement(19008..20879)
                     /locus_tag="ACG8VH_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP9109897.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20900..22018)
                     /locus_tag="ACG8VH_001333"
     CDS             complement(20900..22018)
                     /locus_tag="ACG8VH_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9109898.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22101..22934)
                     /locus_tag="ACG8VH_001334"
     CDS             complement(22101..22934)
                     /locus_tag="ACG8VH_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109899.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22927..23844)
                     /locus_tag="ACG8VH_001335"
     CDS             complement(22927..23844)
                     /locus_tag="ACG8VH_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109900.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24071..25195)
                     /locus_tag="ACG8VH_001336"
     CDS             complement(24071..25195)
                     /locus_tag="ACG8VH_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9109901.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25378..26286
                     /locus_tag="ACG8VH_001337"
     CDS             25378..26286
                     /locus_tag="ACG8VH_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9109902.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26427..28040
                     /locus_tag="ACG8VH_001338"
     CDS             26427..28040
                     /locus_tag="ACG8VH_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9109903.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8890 a         5621 c         4868 g         9214 t
ORIGIN      
        1 ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag agatacgcat
       61 agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt gatttctttt
      121 gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact atatcctcat
      181 ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc ttacagggct
      241 aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag aagaacgggc
      301 aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac aacaatctgt
      361 ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta tcttcttaat
      421 gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac atttagttga
      481 attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat acaacgaaat
      541 tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa atatcaaagc
      601 aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga cagatgcaga
      661 aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta tcggtaaatt
      721 gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg tatcaatcga
      781 cgctggtact aataacgaag aattattgaa taatccatta tacttaggga atcgtcatgc
      841 tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat cagctactga
      901 attattccca gaattgttat tacattggga agattttgga cgaggaaatg ctgcaacgat
      961 cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag gcacaggaat
     1021 cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat tgaaagatca
     1081 aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca tccttttaga
     1141 tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct atcaagtaga
     1201 taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa tcccattcgc
     1261 tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag cagttgtaaa
     1321 agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat tcactgaaga
     1381 aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt tgtctaatcc
     1441 aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg gaaaagcttt
     1501 agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt accaaatcgg
     1561 tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg cttcaacagc
     1621 tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg gaatcgttga
     1681 tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg aattctctca
     1741 aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc gtgaagaaat
     1801 cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata aatctttaga
     1861 agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc taaaaagaaa
     1921 aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc ttctctttgg
     1981 cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa aaacaacaac
     2041 cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa ttaccggcgt
     2101 aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg ctttaggaaa
     2161 attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta atttatttca
     2221 ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct ccgtttttgc
     2281 gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg tagtaaaatc
     2341 cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg ccttgattac
     2401 tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc gttttattcc
     2461 cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag gtatgcttat
     2521 cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg ctggtggtgt
     2581 tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta cggacgcttc
     2641 ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag ctatcattgg
     2701 tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta atggtaaatt
     2761 aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt ccattccaca
     2821 attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa tctgtaacta
     2881 ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta tctgtaaaat
     2941 cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca acttgattgt
     3001 caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga atctaaatgt
     3061 attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg ttatcgcgat
     3121 ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg aatcaactat
     3181 tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg tcttatctgc
     3241 agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg gtggtgccat
     3301 cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa aaatacctga
     3361 tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag gattttcaaa
     3421 aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg gtgtgcttct
     3481 actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt cgcttcttct
     3541 acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga cacagcaagt
     3601 gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc ttgtacatag
     3661 ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt ttctggctcc
     3721 ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata tcctaattgt
     3781 tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg caacatccaa
     3841 atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata ctcctttttt
     3901 cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc ttctaatgtt
     3961 tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac aatcgcttga
     4021 aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc ccacaaacct
     4081 ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg cataaagcta
     4141 cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt agatagagaa
     4201 tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga aacattgact
     4261 ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc acatataagg
     4321 taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta gaagaacgca
     4381 aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt ctcgttttca
     4441 aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac aacagctcca
     4501 ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac ttgtcgcaca
     4561 gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat ccctgcaaca
     4621 gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc catcccggaa
     4681 atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc aatcatcttg
     4741 tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt tattatacag
     4801 taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt tagtgctaaa
     4861 cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt atcgattctt
     4921 ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat atcccattct
     4981 gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc gatgtatgca
     5041 taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa aagaacatag
     5101 ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa tgtttcattt
     5161 ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga atggttatgt
     5221 tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac ctgagccgtc
     5281 taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt ttcgtttcgc
     5341 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttagtcgttc
     5401 atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat atgacatgga
     5461 agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa ctacaaaacg
     5521 gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact cttcttctgc
     5581 ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat cgctcacagc
     5641 aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
     5701 tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg acggcgaatc
     5761 aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag caagtagccg
     5821 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
     5881 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
     5941 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaccctt
     6001 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga ataaattaag
     6061 aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc ctctttccat
     6121 aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca taaagttcat
     6181 aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg tctcataatt
     6241 gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt ttttgtcttc
     6301 aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct cgaaatgagg
     6361 agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa tatcattttc
     6421 gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt ttttaatgtt
     6481 attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata accattgtcc
     6541 gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt ctctcttttc
     6601 agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt tcaatttgtc
     6661 tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt ttgtaaactc
     6721 cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg gcacatccgc
     6781 tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga agatatctga
     6841 atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga cgatccccac
     6901 gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt tccctaaatc
     6961 ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta aaaaaataaa
     7021 accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt ctctactcaa
     7081 tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct taatactcaa
     7141 aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg catctgcggt
     7201 aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt cttattactt
     7261 gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg aacccgcgac
     7321 atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa aaacagtata
     7381 cgcctctttc actccttcct caagtttttt tattctttac gcttccgata tgttttgtag
     7441 taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa agaattagat
     7501 ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa tgcggagatg
     7561 tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt ccgtttggtc
     7621 agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac catccgaatg
     7681 gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa tgatctttca
     7741 cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact gcaagcaatc
     7801 gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt
     7861 ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac tttagtcatt
     7921 ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat caaattttta
     7981 tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt acgttttggc
     8041 ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt tcctggagtt
     8101 caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg agtttctcgc
     8161 ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt tattttccag
     8221 cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt ttcttttagt
     8281 tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc attgatttgt
     8341 tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca aataggcgga
     8401 tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag cgatcttttc
     8461 tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt tcctctagtc
     8521 cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta tcgagaagcg
     8581 gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg tcttgtatta
     8641 tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg
     8701 aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag
     8761 acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt aaccgcagga
     8821 gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt ctcacatttt
     8881 tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat ctgtaaaaaa
     8941 ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg
     9001 ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg atcacaggaa
     9061 tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg ataagaataa
     9121 tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct gaacctctgt
     9181 taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct ctagcgaaat
     9241 aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta gggataaaaa
     9301 acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt aaaaaactct
     9361 gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag attggcaaag
     9421 aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata aaagaagtct
     9481 gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg attaacttat
     9541 tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata aaaatgattg
     9601 gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt actaattgag
     9661 cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt ctttttattt
     9721 tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc agctgtttta
     9781 cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat aaaccttgtt
     9841 gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc ggatgccgat
     9901 ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt ccagcaaatg
     9961 cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt tttttaaaca
    10021 ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga ataatcaaaa
    10081 atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga tcagctcgcc
    10141 atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa
    10201 ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt
    10261 gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga gttaaaccta
    10321 acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca aagttcatag
    10381 caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca
    10441 aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta ttcgtgagct
    10501 gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc ttatacatac
    10561 ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc tagcgtttca
    10621 tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc acctttcttc
    10681 aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt agaaaaaatg
    10741 actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa tgctgcttga
    10801 ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg cagcaacgtg
    10861 ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt ttcattttga
    10921 tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg
    10981 tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa
    11041 atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg attttccgtc
    11101 aatttcattc catctactaa aatcgttccc gatgatggtt caataatact aactaaactt
    11161 ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt
    11221 cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt acttacattg
    11281 atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg tacccttcgt
    11341 tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa cgactaggct
    11401 gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag atatgagtct
    11461 ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct ttagtttatc
    11521 aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta tttcgtttac
    11581 tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat ctttacataa
    11641 atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga gaatctcttc
    11701 atctttcaat actaggccta ttttattttt ataacctggc atgctcatta gataaaggat
    11761 ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc accttttttt
    11821 cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt ggatgctttt
    11881 tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa tctttcgatt
    11941 aataacagtt attgctttat tcggatgaga cgttagataa acaattttac acttttcatt
    12001 cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca aatcgatgtc
    12061 taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa caaattgtat
    12121 aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc tggattcttc
    12181 caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa atatacgcgt
    12241 taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat acaacttttc
    12301 aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac acctttgaaa
    12361 ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa atgaatacat
    12421 catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat
    12481 ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca gagactttct
    12541 cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa tagtctgatt
    12601 ggatggttcc ttctatttta taccattcat catttttaac cgttactcct tccggcatat
    12661 gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg aatcggaaca
    12721 agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca tagctgattt
    12781 ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct aaatagtttt
    12841 catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca ttcatttggt
    12901 ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat tgtgtctgca
    12961 tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg gcttctacga
    13021 tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc aaagctagca
    13081 gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt
    13141 gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact aaagccaaaa
    13201 gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg atatattgat
    13261 cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat aaaatcaaaa
    13321 accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct aagggcaatc
    13381 agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat cagtagattt
    13441 ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt gccgaacaga
    13501 cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat cgtaaaggcc
    13561 aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag cataattcta
    13621 gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa ataacgacct
    13681 gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg gatcagctga
    13741 ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg gtgattttct
    13801 gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata ggcaagccag
    13861 acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc catctgccaa
    13921 gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat cggagcagtc
    13981 agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg atgaacgatc
    14041 ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc agtaaaaata
    14101 gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa aaatacttgt
    14161 aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat aacgatcgat
    14221 aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat gagtttcctc
    14281 ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt
    14341 gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt gactttttct
    14401 gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag ggagaataaa
    14461 aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt catccgttgc
    14521 cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc cacgactaga
    14581 ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg aaacaccaga
    14641 agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc atcaaggaaa
    14701 agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc tactagatga
    14761 actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa aataatcgtt
    14821 gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct
    14881 ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg caagaaatag
    14941 atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc tgacggagca
    15001 ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt taactgattg
    15061 actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat ttgttggatc
    15121 ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac gatgcgccca
    15181 acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact ttgatggaat
    15241 ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac atttgtcaat
    15301 tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc ataaccatac
    15361 ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc actatctact
    15421 tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt atttaaaatc
    15481 cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat aatatgatct
    15541 ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca tcatagcagt
    15601 atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt ctttattcat
    15661 ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac tactaattga
    15721 aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc atgatattat
    15781 tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa aacatacatt
    15841 tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga
    15901 ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc gctaaaaata
    15961 aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat gaaacagttt
    16021 ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc
    16081 tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct tatttaaaag
    16141 aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat gaaaatgaaa
    16201 ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag aaaatcgata
    16261 ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg caacaccctg
    16321 acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt tcctggcttg
    16381 agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac cgttattttt
    16441 tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc cattacaaag
    16501 gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa tggaaggctt
    16561 atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg aaccttgttc
    16621 gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa atggaccggc
    16681 tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata gaaaagcctt
    16741 ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat gtcaccttat
    16801 ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta ggtccgaatg
    16861 gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt atccgtggaa
    16921 aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa gctgatctgc
    16981 atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact ccagaaaaag
    17041 agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa aaaccaatcc
    17101 ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg atgaacaaag
    17161 aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac aaagaagtat
    17221 tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat gaccgatatt
    17281 tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc aaagtctatc
    17341 taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc gccgaattgt
    17401 tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat tattaccaaa
    17461 gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag gttgaagaag
    17521 aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc gcgccagaaa
    17581 acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct gctagacaag
    17641 atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa gaatatgaaa
    17701 attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct ttttttattt
    17761 tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat tactagtcat
    17821 tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat ttaccccatt
    17881 atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt ccgttgtagc
    17941 atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata agttcttttc
    18001 tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca tgatcaccat
    18061 actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc aataacaaat
    18121 ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta aatcaaaata
    18181 tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc atgcttggcc
    18241 tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc ttggttttcc
    18301 atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta ctaaataatc
    18361 tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc cagtttcttc
    18421 actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc taccttgttg
    18481 tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc aaaaacgctg
    18541 aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc ccgttatttt
    18601 ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat tagtgataat
    18661 ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt gttgaaataa
    18721 aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc cttctgtcca
    18781 accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg ggaattgata
    18841 ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc
    18901 attattccat tctttttcca ttaattcttt ataacattca tttatccatt tttctggatg
    18961 tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta gcataatttt
    19021 agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc aaaatcaatt
    19081 ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc aaccactcct
    19141 gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat aaaagtgttt
    19201 tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt tcgtttaata
    19261 gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct atagttttcc
    19321 ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc aaaccctgtc
    19381 tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg ccagccttca
    19441 aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt agaggcaagt
    19501 acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg tccataggtt
    19561 ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg aatccctagc
    19621 attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga aaaaccaaat
    19681 tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa atttggccct
    19741 aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag caaattggct
    19801 tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc cgtccaatac
    19861 tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa tggagataac
    19921 gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat attcaggata
    19981 cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt gactaacccc
    20041 ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga ccaaaagctt
    20101 gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc atcttccgca
    20161 aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat ctggctaatg
    20221 ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc aaaaggatta
    20281 taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag ataacaattt
    20341 tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc aaatttttgg
    20401 ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca ttcatattca
    20461 aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa ttttttccat
    20521 aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac catacgctgg
    20581 tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc agcttttatt
    20641 cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg ccccgctccc
    20701 cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc taccccttga
    20761 tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg ttgatttatc
    20821 tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg atgtttcatt
    20881 gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat cgaagtaagc
    20941 cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt tctctatggt
    21001 atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt gcatagtgct
    21061 tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat cgtacagagg
    21121 aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca ctggtatcaa
    21181 tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt tcgattttct
    21241 gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt tttgtgtaac
    21301 gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa caagaattct
    21361 cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca tcaaattctg
    21421 cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta tctgcatgct
    21481 gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa tacagtaatg
    21541 gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc catgctacta
    21601 aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt aaccctagtt
    21661 gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt aagaataaaa
    21721 aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat aattgttctt
    21781 gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc tgccataaaa
    21841 taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt acgtctgtat
    21901 attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat tccgcatcta
    21961 tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt tcattcatgg
    22021 atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac gaactagatg
    22081 gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc cttaaattta
    22141 gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa aattagcata
    22201 attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt gataattaag
    22261 ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata ttgattccaa
    22321 cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat cggtacagcg
    22381 attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc catcagtgct
    22441 gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa
    22501 gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat gtaaaataaa
    22561 ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt gaacaacagt
    22621 ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga actggctgta
    22681 gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa gggataatta
    22741 tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt gaaattgatt
    22801 ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata taaatatggc
    22861 atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct gctttgtatc
    22921 ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata atataacaga
    22981 tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca tttcaaaact
    23041 tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat tggaagtccc
    23101 aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta aaactgtggc
    23161 cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc gaaatttcga
    23221 tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa gaccagctaa
    23281 aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca ttgatattcg
    23341 tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat tcaaaaatcc
    23401 attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag ccaccgttga
    23461 tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta actgtgctga
    23521 attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg atcctgccat
    23581 gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga tagctagata
    23641 attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa aagcaaacga
    23701 gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc
    23761 ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct ttattgtctt
    23821 gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa tcaccagtta
    23881 attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat
    23941 cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca tctgaataag
    24001 tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt tcatagtatt
    24061 tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag gccaatgagt
    24121 tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct gtcagcataa
    24181 atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca taaaaacttg
    24241 atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc agtggagcca
    24301 tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca taccagccgc
    24361 cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat
    24421 gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc gcttctttag
    24481 tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg gctagcatgt
    24541 tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta ccagtttttt
    24601 cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct tcaaaatcta
    24661 cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta aaatcactag
    24721 cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat ttttgatcat
    24781 aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa aaagcatctt
    24841 gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata tccggcatat
    24901 cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc gaagctacat
    24961 tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt gcagcatttt
    25021 caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg gcttttttta
    25081 tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat gcacttaagc
    25141 tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt ttcatgtcta
    25201 tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata ttaacggaat
    25261 tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct tttcattttt
    25321 gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga aaggaaagtg
    25381 gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact aacctattcc
    25441 atagataccc atcttcattt atctgctgct ccctattttc gactactaat aaatgaaagc
    25501 gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc ttccctttta
    25561 ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac acaaactttt
    25621 atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg acaatctttt
    25681 tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca gttcaaaatt
    25741 gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa ttcttttttc
    25801 atactatgcc aacagatgag cattcatttg ctttccttat tagttactta ttgtctgcaa
    25861 aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt attgacaata
    25921 ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga tgaattagtt
    25981 gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct tgaacaatta
    26041 gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa agtgaatgga
    26101 aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg tctcgtgcta
    26161 gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc gatccatcat
    26221 ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta tctatcctta
    26281 aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt tctgtctatt
    26341 caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata acatagtacg
    26401 atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt gtagctggcg
    26461 gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc attatctatg
    26521 aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta ggaggtgtga
    26581 tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca aaaaacaaca
    26641 tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag cgattaacag
    26701 ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg atcatctctt
    26761 ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa aacggctttg
    26821 tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac
    26881 aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag aatttcaagc
    26941 atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc ccttatgatc
    27001 cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc gttcatttag
    27061 aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca gatggctccg
    27121 tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat gaagtggtga
    27181 aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac cagttacaaa
    27241 ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt gtagtgactg
    27301 gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat atgctggcag
    27361 atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa
    27421 tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca
    27481 taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc tatccagaag
    27541 ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt ttaggtggac
    27601 aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta gcaatcaccg
    27661 gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca ccgtattcta
    27721 ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg acgaatatcg
    27781 tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg ttctttttag
    27841 atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa aatattccaa
    27901 tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt tacattactt
    27961 tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg ggtatcaaag
    28021 cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct aggagttatt
    28081 tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat gtatatagaa
    28141 tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg ttatagttta
    28201 agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat aaaaaaacat
    28261 gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac tacgcctaat
    28321 attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc cctttcgaat
    28381 attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa tcgactggct
    28441 tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg taatcatatt
    28501 tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt gtgtattaaa
    28561 taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DAZKCC010000029        28156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000029 DAZKCC010000000
VERSION     DAZKCC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.029"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..1498)
                     /locus_tag="ACG8VH_001339"
     CDS             complement(554..1498)
                     /locus_tag="ACG8VH_001339"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9109904.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1794..2738)
                     /locus_tag="ACG8VH_001340"
     CDS             complement(1794..2738)
                     /locus_tag="ACG8VH_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9109905.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2959..4173
                     /locus_tag="ACG8VH_001341"
     CDS             2959..4173
                     /locus_tag="ACG8VH_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9109906.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4195..6210
                     /locus_tag="ACG8VH_001342"
     CDS             4195..6210
                     /locus_tag="ACG8VH_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFP9109907.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6293..7666)
                     /locus_tag="ACG8VH_001343"
     CDS             complement(6293..7666)
                     /locus_tag="ACG8VH_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9109908.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7692..7976)
                     /locus_tag="ACG8VH_001344"
     CDS             complement(7692..7976)
                     /locus_tag="ACG8VH_001344"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9109909.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7978..9993)
                     /locus_tag="ACG8VH_001345"
     CDS             complement(7978..9993)
                     /locus_tag="ACG8VH_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9109910.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10172..10477)
                     /locus_tag="ACG8VH_001346"
     CDS             complement(10172..10477)
                     /locus_tag="ACG8VH_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP9109911.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10566..11597)
                     /locus_tag="ACG8VH_001347"
     CDS             complement(10566..11597)
                     /locus_tag="ACG8VH_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9109912.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11804..12679
                     /locus_tag="ACG8VH_001348"
     CDS             11804..12679
                     /locus_tag="ACG8VH_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9109913.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12831..13673)
                     /locus_tag="ACG8VH_001349"
     CDS             complement(12831..13673)
                     /locus_tag="ACG8VH_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9109914.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13765..15642)
                     /locus_tag="ACG8VH_001350"
     CDS             complement(13765..15642)
                     /locus_tag="ACG8VH_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9109915.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15639..17060)
                     /locus_tag="ACG8VH_001351"
     CDS             complement(15639..17060)
                     /locus_tag="ACG8VH_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9109916.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17063..20452)
                     /locus_tag="ACG8VH_001352"
                     /pseudo
     CDS             complement(17063..20452)
                     /locus_tag="ACG8VH_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
     gene            20709..22100
                     /locus_tag="ACG8VH_001353"
     CDS             20709..22100
                     /locus_tag="ACG8VH_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109917.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22173..22820)
                     /locus_tag="ACG8VH_001354"
     CDS             complement(22173..22820)
                     /locus_tag="ACG8VH_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9109918.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22828..23514)
                     /locus_tag="ACG8VH_001355"
     CDS             complement(22828..23514)
                     /locus_tag="ACG8VH_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109919.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23520..24593)
                     /locus_tag="ACG8VH_001356"
     CDS             complement(23520..24593)
                     /locus_tag="ACG8VH_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109920.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24815..25489
                     /locus_tag="ACG8VH_001357"
     CDS             24815..25489
                     /locus_tag="ACG8VH_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9109921.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25494..26834
                     /locus_tag="ACG8VH_001358"
     CDS             25494..26834
                     /locus_tag="ACG8VH_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9109922.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26983..27969)
                     /locus_tag="ACG8VH_001359"
     CDS             complement(26983..27969)
                     /locus_tag="ACG8VH_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP9109923.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8242 a         5282 c         5092 g         9540 t
ORIGIN      
        1 attaattttg aaataattga tttaaaagga atacaaaaga gtgaatagta ggaaatagtg
       61 gtttagaact agtgggctga ttttttatat attttagttt acataatata tattatacaa
      121 agtagattta aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt
      181 tccatcaaaa tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt
      241 tgtcgtttaa ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa
      301 ttagatatga atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa
      361 cattgataac gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg
      421 gatacatttt cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat
      481 caataaaaaa ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca
      541 tattcattgt ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc
      601 tgaacattgc tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt
      661 cgtcaacgat tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat
      721 aagcagatac aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag
      781 tactcatacc aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat
      841 tacggacttt atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta
      901 caatttcaaa cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat
      961 caccatgttc acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg
     1021 cgatttcttt acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg
     1081 ttggcagacc agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa
     1141 caacgataat accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg
     1201 acgcatttgt tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg
     1261 tgataacaac gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat
     1321 tttcatcgcc ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt
     1381 gattcacatc gatcaatact aattcattag agattccttg gttgatcatt gcataagcga
     1441 tacttgttcc aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga
     1501 ataaacagct ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt
     1561 cattttatca acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc
     1621 ctgaatacgc ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac
     1681 caaaagttta gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag
     1741 caatcgcacc taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt
     1801 ttatttctcc attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa
     1861 gtcgtttgtt tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga
     1921 atcagttcca acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca
     1981 aaatacattt tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt
     2041 tcttttaacg tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca
     2101 ttttctgcgc atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg
     2161 atcagtaata gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa
     2221 taaatcaaca gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa
     2281 tgaatgaatt gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt
     2341 agattatttg tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat
     2401 aagatgttga ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta
     2461 tcaatgatca ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa
     2521 catgtaccgc tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc
     2581 gcatatcgac ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg
     2641 atttttggcg gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt
     2701 tcacgctgca gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag
     2761 ttccaattga ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac
     2821 tgctattata tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa
     2881 cttcctgtga ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct
     2941 tacaaaaatg aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca
     3001 gtctctctta ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct
     3061 acttatgcca cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg
     3121 gtcactattt ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact
     3181 gtgatcatcg gattagtgat tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt
     3241 tacagtattg tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct
     3301 ttggcagtca gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt
     3361 tttcaaagtg catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta
     3421 gtacagttcg gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc
     3481 ttgtttgttt tgtttgttcc tacacctgaa acaactgaac ttcaatcgca tggcacagcc
     3541 ccaacaagac aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg
     3601 ctggtcatcg tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat
     3661 tctctaggaa acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg
     3721 gctgccggag tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt
     3781 tctttactac tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg
     3841 attgggtttg gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc
     3901 aaccaagtaa gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg
     3961 gttggcgcta atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct
     4021 tctcttggga gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc
     4081 gcgcttatta gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat
     4141 gaagaaataa aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca
     4201 aatgaaaaac ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga
     4261 ctgctacaaa atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg
     4321 gatgattgcc atcggaacgt caagtttaag agatattact ttcttgatca accttccatg
     4381 tttaaagtga cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt
     4441 ggaaaatggc tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca
     4501 tcagggctac cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca
     4561 gcaagtaatt ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca
     4621 aaagatccaa atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag
     4681 attggcggag gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata
     4741 tctctggatg accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct
     4801 atggatatcg attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt
     4861 aaacgcgctt ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa
     4921 gtttttcatg aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta
     4981 ctgaatcaga tctggtcagt atcagaagaa gccttcaagt tatgtagtga tttgtttttt
     5041 atcgacggta tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg
     5101 atcaatcaat atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga
     5161 ggacaaaatg aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg
     5221 attggtatcg aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac
     5281 cctaagatgc aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt
     5341 tatgggagag caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg
     5401 cttgctgcag aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat
     5461 atcttagctg aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa
     5521 tatcaactat tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt
     5581 atcgattgct atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt
     5641 ttattcgatt ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat
     5701 gacactatca cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc
     5761 aaactcggac agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta
     5821 tctcatgatg cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag
     5881 tacagtatgt atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca
     5941 atctatcttt taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa
     6001 tttatgatac aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa
     6061 gatggaaaga tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca
     6121 ggcggaacag tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc
     6181 ttaacaagct attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag
     6241 actttaaatc ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct
     6301 gttctttaga aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa
     6361 gtaaaaagaa agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc
     6421 caagtacaag tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa
     6481 gatctcctaa tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag
     6541 cagaacctaa taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag
     6601 ctccgcagaa gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca
     6661 tagcaaacat ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat
     6721 taggtgcata tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt
     6781 ttttagcaat cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa
     6841 ggacgataac aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt
     6901 tgtctccatt gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga
     6961 catatacgat cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac
     7021 ttttaggaat ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc
     7081 atccactaga agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag
     7141 tgatgttctt cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga
     7201 agaccccgcc taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga
     7261 ctgctgttag acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc
     7321 ttgtgaatct agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag
     7381 gtgtaccgta ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt
     7441 gagtatgcag agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac
     7501 cagcaccgcc gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt
     7561 ctgaaaattt cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg
     7621 agggctgact gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc
     7681 ttgaatcatt tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg
     7741 tcaatgatcg attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc
     7801 aaagaatgct caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta
     7861 tgacttactt caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc
     7921 atctctacca taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca
     7981 ttcgtctcct tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc
     8041 ttgaagattt tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc
     8101 ctcattatcg atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag
     8161 cagatccaca ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc
     8221 tggacgagca tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc
     8281 cacgcgctcg atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg
     8341 ctgtgcggca aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat
     8401 ttcttctgtt aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt
     8461 gttcatttta cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc
     8521 gatataaggc agtagcgccg acaacaattc ttgggcagaa ggcattgaaa atcctggtat
     8581 cagccaatct ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg
     8641 atgcagaaca tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat
     8701 aatatcgtaa gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa
     8761 aaagatcatc gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc
     8821 acttggacag acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct
     8881 gatctctccg ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc
     8941 tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg
     9001 atgcttaatt tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata
     9061 ggcagggacc aagtgataaa agacattcat caataattca cgaaacgaag caaattctac
     9121 ggccatcagg aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag
     9181 tgcaggctcc tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat
     9241 ctccgaattt tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt
     9301 gactgcaaga gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat
     9361 tcgaatggtt gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg
     9421 aagatctgat agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat
     9481 ggaaagggtt gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg
     9541 agcagtgcga ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc
     9601 agttttccta gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt
     9661 gatatctgac aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga
     9721 aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt
     9781 atacacagct tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa
     9841 tttctttata cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat
     9901 cgcctcgctt gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg
     9961 tgttttcagg agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc
    10021 tagtatggta gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt
    10081 tacgactgta ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg
    10141 tctcctgctt ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg
    10201 tcagttcttc aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt
    10261 gattgggctc attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt
    10321 ctttattttc tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt
    10381 caccagaatt agttcctgct aaagccactt cttcttctgt tttaggggcc attgaacctc
    10441 ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta
    10501 tactttttca tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa
    10561 atatgtcata tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc
    10621 atttgtaaga ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg
    10681 taaatccctt cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga
    10741 taactccctt gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca
    10801 aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt
    10861 tgtttcacga acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg
    10921 aaataactgg attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa
    10981 tcattcattc gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg
    11041 ccgaaataag aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc
    11101 gcagtagaaa attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt
    11161 tctacttcta aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag
    11221 tcagaatcga accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat
    11281 gcaaataact cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc
    11341 ttaccatgtt cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg
    11401 acaactaatt ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg
    11461 tcttcgaatg ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg
    11521 ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga
    11581 taagcgattg taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa
    11641 aagcgcagaa agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta
    11701 tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg
    11761 gaaccaagtg tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa
    11821 actaacaaaa caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag
    11881 gaatatcgaa gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc
    11941 ccattcagct atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg
    12001 tttcgagctg gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc
    12061 taattttcct ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt
    12121 atcgatccag tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat
    12181 cggtaaagta ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat
    12241 cacggcaagg aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga
    12301 agaatacagt gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt
    12361 tattagctac agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt
    12421 agaggcccct accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga
    12481 tatgtgtctt gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc
    12541 tcaaattgcc ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt
    12601 tgaagagaat atccgcaact tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa
    12661 agaatatcgg aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat
    12721 aggctctctt tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct
    12781 gtttgtttac ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg
    12841 tttcttattt tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat
    12901 gcagctaaca atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt
    12961 tgaacttctt ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg
    13021 accatataag gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga
    13081 tcttttgtat cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt
    13141 ccattgattt cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac
    13201 tttgcttgag gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta
    13261 ggataagacg ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca
    13321 tcaaaaattg gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc
    13381 tcaaaatagg ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct
    13441 ggatttccac cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta
    13501 gccatgactt cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga
    13561 tccagataat tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag
    13621 agaagcaaga aaagcatcag cagatctaga agtttccttt tctttggttt catttgtgtt
    13681 ctccttgtga tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc
    13741 ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag
    13801 tccagcaatc agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc
    13861 tgttgaagga agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc
    13921 tgctgttaca taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata
    13981 tttttctggc gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc
    14041 gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct
    14101 gacggctttt gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg
    14161 aacgacgaat tctgctccag caagtgtttg gtctgatgta acgtcaccat ctactttaac
    14221 aaatcgtttg cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc
    14281 gttatcgaca tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg
    14341 catatagtaa acgaatttca atgtaccgcc aggagttaat gaaggaatat aattcggatc
    14401 aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa taatttactg ggtcgatttc
    14461 tttatttcca tcatataagg cataagcata cgttcctgaa gaatcattat caaatgttaa
    14521 agcagcgtca tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt
    14581 atcagcgata cctaatggga tattgacaga aatctcatat tgaatttttt caccgattgc
    14641 gacatctttt ccattcaatt gaggtgttgt tttatcaact ttagacgtat catttttgat
    14701 ggtcttttct actggtgttt ggctatttgc caggatctca aaaggcgtga ttgtttgctt
    14761 ttcaatcatt tccgcattat caggagcttt tacttcttct aaattatatt ttccaacttc
    14821 taaatgatta acgatcaact ggcctttttc aatagatgcc tcggcaaagt cattgttgcc
    14881 gatgtcataa gaatgaccag taatgaaatg tttggctttg gtttgatcag ttgtccaagt
    14941 gtacaatcca tctgtgacac tttggatgta ttttttgacg cttggtgttc cttcttcttt
    15001 agaaataata aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac
    15061 tttcaacgtt ccatcattac cgactgtatt tttagggtag agatggatag tatctagttc
    15121 ttctgtcccg tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc
    15181 taaaaccatg tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac
    15241 tgcatctttc ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt
    15301 cgttcctgaa gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc
    15361 cacggacgct cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa
    15421 tgaaatatcg gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg
    15481 gattaaagga tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt
    15541 ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa
    15601 aaggactcct aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt
    15661 tttttgataa acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt
    15721 gtatttttcg tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact
    15781 actttcaata tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga
    15841 atttctgttt cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt
    15901 cctgttttca acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca
    15961 tctgattcgt tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct
    16021 ttgtttttaa taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt
    16081 ttgacaaatt gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca
    16141 aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc
    16201 atctgataag aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat
    16261 aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca
    16321 ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac
    16381 aattcgaact tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg
    16441 attttttcac caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga
    16501 tcagggtagt cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg
    16561 ttttgtccat gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct
    16621 ggtactttgg attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc
    16681 agagaaaaag aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta
    16741 aacataccgg aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg
    16801 cccttttccc gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca
    16861 tttaatcctc gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc
    16921 ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct
    16981 gtttctgttg ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta
    17041 agccaaccaa gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc
    17101 aaatagaaaa cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc
    17161 ccgtttcagg taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt
    17221 tggctcctgg tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt
    17281 cgtgtataac tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt
    17341 cggtcagcgt ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc
    17401 catcttttgg cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta
    17461 atgtctgtcc gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt
    17521 gatgagtgag cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt
    17581 ttccatattt atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac
    17641 cagctgtttc aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt
    17701 tttcattcgt tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta
    17761 gggaaaatgc tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac
    17821 gtactgctac aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg
    17881 aagtagtgac tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc
    17941 aagtagtctt tttgcttaat ccatgccctt atggagcttt tgtttctgtc agcgtatagg
    18001 tcatttcttt ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg
    18061 ttagtaaagt atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt
    18121 cacctgtaga ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt
    18181 tccatgtcat tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg
    18241 tttggtaact gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat
    18301 aactttctcc gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg
    18361 tatctttagc tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg
    18421 tatgttcccg ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa
    18481 attcttccca taactttttg atgtgaagac tgactccggg agctttagca gatggtatac
    18541 cgaattcagc taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt
    18601 gataccagaa atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga
    18661 tttggatttc ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta
    18721 tggaaataga tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac
    18781 caggctgata aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg
    18841 ctgagatctg tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg
    18901 attcatagta aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta
    18961 cttctgcttt tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta
    19021 tttcgattcc tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt
    19081 cgattcttct ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag
    19141 tatttcccgg ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt
    19201 gtgcgtgtac ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga
    19261 tcactttttt atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt
    19321 gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg
    19381 aatcaaatga acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc
    19441 cgacatatcc catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac
    19501 gatcgacacc aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt
    19561 ctacgaccaa tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca
    19621 aataaacatc aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt
    19681 ctgcttgatt accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc
    19741 cattccaaga atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa
    19801 gaacatttgt atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg
    19861 ttgtatattc tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg
    19921 taggcaatgt aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt
    19981 cgtctggctt tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt
    20041 catattcttt attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga
    20101 ttggaaaact cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc
    20161 cgctatttac ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat
    20221 tttcgactag accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct
    20281 tgatgttcag tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta
    20341 aaactgttgg atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac
    20401 tcaatattat cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc
    20461 tcctttttct ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt
    20521 tacataattt tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca
    20581 caccaatttt cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt
    20641 ttcattgaaa gaaaagggtt gagggagaaa aacatatgat caggttgttg gaaggaaact
    20701 atagaaagat ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa
    20761 aagaacttgc caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga
    20821 tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc
    20881 ttgaacattt gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta
    20941 caaacagtcg tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt
    21001 gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa
    21061 ttttagaaag ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta
    21121 aaagtgagct ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac
    21181 gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt
    21241 gtcagatgaa tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt
    21301 ttgttgtgca tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact
    21361 cttatgtaca gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag
    21421 gaaaattgaa gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt
    21481 cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct
    21541 actgttcgtt ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat
    21601 taacgcaaat ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc
    21661 gtgcaactta ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc
    21721 gtgcagaact tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta
    21781 gctatatctt ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa
    21841 aaatactgat tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca
    21901 gtgatattct tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc
    21961 tgtcgaaata caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg
    22021 cccatgttcc tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca
    22081 tccgcttatt cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa
    22141 atttcaggac aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga
    22201 gatttatagc gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc
    22261 gtcaaagtgt tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac
    22321 gattgaatgg agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa
    22381 tcagccgata gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat
    22441 aacctttgcc aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg
    22501 atccccaaaa agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact
    22561 atgtgagaac tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta
    22621 taaaaagcac cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg
    22681 atgtgctgtg agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc
    22741 gataaattga aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt
    22801 ggatagccct tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta
    22861 cccatcttcc attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac
    22921 cataatagtc gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata
    22981 tgcatgatct gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt
    23041 atataaagcg cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata
    23101 actattttta agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc
    23161 ttcttttttt gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg
    23221 aatcaaatta gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt
    23281 tttttctgga agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt
    23341 ttgcaatcct cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat
    23401 gctgacgaat tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt
    23461 atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat
    23521 taacctccta ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg
    23581 cctagcattg cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca
    23641 gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc
    23701 agaacgccta ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa
    23761 tttttgatcc cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg
    23821 tacatgaaaa taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt
    23881 aaggtcatat tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat
    23941 aactgtagac ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc
    24001 ccattgatcg gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc
    24061 cacgtatcta gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata
    24121 ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag
    24181 cctgaatcag ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct
    24241 ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct
    24301 ttgttttttc ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca
    24361 atgtatttca gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc
    24421 gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt
    24481 ccattcgcta gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg
    24541 atcaagccat atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt
    24601 tcctccctat gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa
    24661 gtgacaattt gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc
    24721 gataaaacct taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga
    24781 taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga
    24841 cgataaagta ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca
    24901 agtttatgat ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat
    24961 cttggatttg atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga
    25021 aaaaatacag actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga
    25081 aggatttgaa cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat
    25141 catgcgcgtg aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa
    25201 gtataaggat atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt
    25261 gcccatacaa ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat
    25321 tttgacaaaa gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac
    25381 agttattgaa gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa
    25441 agaaatcaaa accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg
    25501 gagatattac acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt
    25561 ttatcttttt tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag
    25621 aaacagatct gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac
    25681 aaagatatca gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa
    25741 acatggaaca gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt
    25801 ggtctaaaaa tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca
    25861 aagaattaaa aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag
    25921 acagtggctc atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag
    25981 atggcgttgc ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga
    26041 cttttcgcac gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca
    26101 gttattactt gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag
    26161 aatttgttga gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt
    26221 tggaacatct ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc
    26281 aagcattgtc agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc
    26341 ggaatgattc tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg
    26401 aacaacttgt taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc
    26461 ataacgatgc aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta
    26521 ttttgctgga taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat
    26581 ctgttaacca taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag
    26641 aagatcaagc aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga
    26701 ctggcggata cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac
    26761 agatttctgt atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga
    26821 agcctaaaaa ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc
    26881 ttttttgttg cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt
    26941 ctttaagctg accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg
    27001 gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc
    27061 tgtcactttt tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac
    27121 tgtatgtttg cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc
    27181 caatttttcg tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat
    27241 ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat
    27301 gtcgacttct ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg
    27361 aattgtatca tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat
    27421 tgtatcagtc tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc
    27481 cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt
    27541 ttctaatcca gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc
    27601 ttctgttgtt tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat
    27661 atctcctcta tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt
    27721 atcaaaagaa aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat
    27781 ccgattttca tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc
    27841 tgcttcatca gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg
    27901 ttcaattact gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt
    27961 ctcttccatg taaattcctc ctagcaaata gatttatgat tggtatattg ttacttataa
    28021 atttattcta taggagacga ttgttttcta caaatattta gattggaatt tgattaaaac
    28081 aatttaattg aaatttgaaa acatttatgt taatgaaagt catattctaa taaaatgtca
    28141 tttgtttcta tatgaa
//
LOCUS       DAZKCC010000030        27472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000030 DAZKCC010000000
VERSION     DAZKCC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.030"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG8VH_001360"
     CDS             275..1753
                     /locus_tag="ACG8VH_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9109924.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="ACG8VH_001361"
     CDS             2030..4090
                     /locus_tag="ACG8VH_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP9109925.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="ACG8VH_001362"
     CDS             4173..4742
                     /locus_tag="ACG8VH_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109926.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="ACG8VH_001363"
     CDS             complement(4917..5723)
                     /locus_tag="ACG8VH_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109927.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="ACG8VH_001364"
     CDS             5861..7375
                     /locus_tag="ACG8VH_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP9109928.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7778..8761)
                     /locus_tag="ACG8VH_001365"
     CDS             complement(7778..8761)
                     /locus_tag="ACG8VH_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP9109929.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8766..9413)
                     /locus_tag="ACG8VH_001366"
     CDS             complement(8766..9413)
                     /locus_tag="ACG8VH_001366"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9109930.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9498..10130)
                     /locus_tag="ACG8VH_001367"
     CDS             complement(9498..10130)
                     /locus_tag="ACG8VH_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9109931.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACG8VH_001368"
     CDS             complement(10146..10697)
                     /gene="coaC"
                     /locus_tag="ACG8VH_001368"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP9109932.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10694..11431)
                     /locus_tag="ACG8VH_001369"
     CDS             complement(10694..11431)
                     /locus_tag="ACG8VH_001369"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP9109933.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11428..12417)
                     /locus_tag="ACG8VH_001370"
     CDS             complement(11428..12417)
                     /locus_tag="ACG8VH_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9109934.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12675..13217
                     /locus_tag="ACG8VH_001371"
     CDS             12675..13217
                     /locus_tag="ACG8VH_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9109935.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13234..14319
                     /locus_tag="ACG8VH_001372"
     CDS             13234..14319
                     /locus_tag="ACG8VH_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9109936.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14331..15137
                     /locus_tag="ACG8VH_001373"
     CDS             14331..15137
                     /locus_tag="ACG8VH_001373"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109937.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15138..15965
                     /locus_tag="ACG8VH_001374"
     CDS             15138..15965
                     /locus_tag="ACG8VH_001374"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9109938.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15962..17035
                     /locus_tag="ACG8VH_001375"
     CDS             15962..17035
                     /locus_tag="ACG8VH_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9109939.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17463..20159
                     /locus_tag="ACG8VH_001376"
     CDS             17463..20159
                     /locus_tag="ACG8VH_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9109940.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20224..20724
                     /locus_tag="ACG8VH_001377"
     CDS             20224..20724
                     /locus_tag="ACG8VH_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9109941.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            20736..21230
                     /locus_tag="ACG8VH_001378"
     CDS             20736..21230
                     /locus_tag="ACG8VH_001378"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9109942.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21251..22060
                     /locus_tag="ACG8VH_001379"
     CDS             21251..22060
                     /locus_tag="ACG8VH_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9109943.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22057..22878
                     /locus_tag="ACG8VH_001380"
     CDS             22057..22878
                     /locus_tag="ACG8VH_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9109944.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22929..25028
                     /locus_tag="ACG8VH_001381"
     CDS             22929..25028
                     /locus_tag="ACG8VH_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP9109945.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            25025..26143
                     /locus_tag="ACG8VH_001382"
     CDS             25025..26143
                     /locus_tag="ACG8VH_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9109946.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26182..27060
                     /locus_tag="ACG8VH_001383"
     CDS             26182..27060
                     /locus_tag="ACG8VH_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9109947.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27206..27403
                     /locus_tag="ACG8VH_001384"
     CDS             27206..27403
                     /locus_tag="ACG8VH_001384"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9109948.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8779 a         4712 c         5747 g         8234 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     7501 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     7561 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     7621 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     7681 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     7741 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     7801 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     7861 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     7921 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     7981 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     8041 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     8101 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     8161 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     8221 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     8281 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     8341 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     8401 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     8461 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     8521 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     8581 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     8641 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     8701 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     8761 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     8821 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     8881 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     8941 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     9001 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     9061 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     9121 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     9181 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     9241 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     9301 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     9361 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     9421 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     9481 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     9541 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     9601 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     9661 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     9721 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     9781 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     9841 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     9901 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     9961 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
    10021 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
    10081 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
    10141 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
    10201 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
    10261 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
    10321 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
    10381 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
    10441 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
    10501 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
    10561 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
    10621 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
    10681 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
    10741 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
    10801 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
    10861 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
    10921 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
    10981 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
    11041 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
    11101 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
    11161 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
    11221 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
    11281 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
    11341 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
    11401 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
    11461 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
    11521 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
    11581 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
    11641 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
    11701 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
    11761 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
    11821 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    11881 aaaggtaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    11941 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    12001 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    12061 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    12121 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    12181 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    12241 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    12301 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    12361 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    12421 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    12481 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    12541 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    12601 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    12661 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    12721 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    12781 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    12841 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    12901 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    12961 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    13021 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    13081 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    13141 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    13201 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    13261 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    13321 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    13381 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    13441 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    13501 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    13561 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    13621 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    13681 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    13741 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    13801 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    13861 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    13921 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    13981 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    14041 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    14101 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    14161 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    14221 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    14281 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    14341 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    14401 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    14461 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    14521 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    14581 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    14641 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    14701 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    14761 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    14821 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    14881 gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    14941 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    15001 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    15061 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    15121 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    15181 ttttattctt atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    15241 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    15301 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    15361 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    15421 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    15481 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    15541 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    15601 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    15661 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    15721 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    15781 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    15841 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    15901 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    15961 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    16021 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    16081 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    16141 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    16201 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    16261 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    16321 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    16381 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    16441 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    16501 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac
    16561 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    16621 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    16681 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    16741 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    16801 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    16861 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    16921 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    16981 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    17041 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    17101 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    17161 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    17221 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    17281 actatgcaaa tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag
    17341 ttcaatattt gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta
    17401 gataaattat tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa
    17461 aattggaata caaatataga atagacatat tgcagccgat agtttattat gcttttagac
    17521 acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga
    17581 tacgactaac agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta
    17641 tctctcgtca gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga
    17701 aattaccggg aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata
    17761 cggagcaaat aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg
    17821 tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa
    17881 atggagccac gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc
    17941 atgagcaaat tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg
    18001 acaatcccga actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg
    18061 ctgaaaagga taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg
    18121 aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa
    18181 actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg
    18241 ccacgacgga aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta
    18301 gtgttatgtt gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat
    18361 tattttttca aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct
    18421 tgtctttaat gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta
    18481 aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa
    18541 atgagatctt gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta
    18601 gtttattgat aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg
    18661 attcgatttt ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt
    18721 cttcgataaa gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat
    18781 caatgagtag aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa
    18841 aaacgtacgg attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca
    18901 tttccccaag ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac
    18961 ctcggacgac ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg
    19021 acgagaactt tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca
    19081 tcgagctgaa gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag
    19141 tagtgaatca attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta
    19201 atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata
    19261 gtctagtgct gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg
    19321 atttaaaagg ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg
    19381 gcttgaagat gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt
    19441 attcaatcaa tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct
    19501 gtatgtcggg tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg
    19561 atgaatccat cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa
    19621 atgatgaaac ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt
    19681 ccttttcaat tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt
    19741 tggagcatat tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc
    19801 tagtaaaatt cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga
    19861 tcgtgatgag tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt
    19921 taaatggccg tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat
    19981 tcttggctcg gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg
    20041 aattttatta tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag
    20101 tcaatgctta tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa
    20161 gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt
    20221 aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac
    20281 agaaagaaag gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct
    20341 tcaggctttc ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt
    20401 aatgcttatg tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg
    20461 aacgagcagg atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag
    20521 attaccaaaa cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg
    20581 ccaattctgc tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa
    20641 ctggtaaata aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta
    20701 aatgaagagg aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg
    20761 aacgattaat tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt
    20821 tagtagtagc aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg
    20881 ctgcaccatc gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa
    20941 aagatccgcg ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg
    21001 taaaagttgc gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga
    21061 aaatgacacc gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt
    21121 cagaaattca agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta
    21181 caccggcgat ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag
    21241 gagaaaaatt atgtatgatg catttgtagt cgctttggcc gtttttattg gagttgctgg
    21301 acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg
    21361 agcgttgatg ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt
    21421 tatgggtgtc gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct
    21481 ggctacagcg ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt
    21541 tccactagca gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc
    21601 gatggctgct aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct
    21661 gcacttcttt atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt
    21721 tttatttgga gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa
    21781 cggattaaca gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat
    21841 gctttggagt aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct
    21901 cgagttacca ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca
    21961 aagagatcta caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa
    22021 tgcaacgctt gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct
    22081 aaagatgaga aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg
    22141 gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat
    22201 catttctaca aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt
    22261 tcgacgacca ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa
    22321 aattctgaaa aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg
    22381 ggaccattgg ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg
    22441 ggactagcga tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt
    22501 atgttcaata tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta
    22561 ggctctaaat atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct
    22621 gctagtatct tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat
    22681 accgttttga gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat
    22741 agtattttta aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat
    22801 aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca
    22861 ttacttggaa tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga
    22921 gggaaaagat gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg
    22981 agttaaacga cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg
    23041 atgtaaaggg attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt
    23101 gcgggacgat tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag
    23161 gtgtttgggt caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa
    23221 acgggattgt ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag
    23281 actgtttgcc tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg
    23341 ctcccatact ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg
    23401 aaacacacga aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt
    23461 cggatcagca aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg
    23521 aagtggcaag cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt
    23581 ttcaggaatc tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat
    23641 ttgggcagct gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc
    23701 aaagcttttc tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca
    23761 gctatcctgc tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca
    23821 ccatgtttga accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta
    23881 agtttcctgg ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca
    23941 gtgcgttact ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt
    24001 gtccgcaggt tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt
    24061 caggggaacc aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt
    24121 atcataccgg cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg
    24181 aagccctaga acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg
    24241 aatcaatttg taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa
    24301 tcggtgatga ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc
    24361 aagggttaat ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg
    24421 atggagaaga atcagacact acgttgatgc ctttttatca atttttagat aataaagatc
    24481 atttatatca gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg
    24541 aaatcaaagg catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg
    24601 ttttaatgag cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt
    24661 gtttggcaga tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag
    24721 gagaagtcgt gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca
    24781 gcctaatgat cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc
    24841 ctgttgataa tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga
    24901 ttgaatgtac gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa
    24961 gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa
    25021 aaagatgagt atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac
    25081 tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc
    25141 aagccgcaaa ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga
    25201 tattactgga acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt
    25261 tggcagtttt gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc
    25321 tgctgttgaa agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt
    25381 gaactcgttg ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc
    25441 tttccaaaca tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt
    25501 tttttatacg ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca
    25561 cgcaaattgg catcgggaac gcattaccaa attacaggag gattataagt taattgatcg
    25621 attaaagggt caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta
    25681 ttggtggggc gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt
    25741 aacctcaaca ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga
    25801 gttcggacgt ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc
    25861 aaatagagat gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt
    25921 tggtgacgac gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg
    25981 ttttaccgaa gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt
    26041 tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa
    26101 tactaaacgt catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat
    26161 attcgaaggg aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt
    26221 gtctgtgcag tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca
    26281 actccagttg aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg
    26341 gcaggcatcg gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc
    26401 ggtcatatca catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta
    26461 aagcaatatt tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt
    26521 gaatatgttg ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca
    26581 ggtatcggag gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa
    26641 atggggcata tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc
    26701 caccaagatt gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta
    26761 aaaggccaga atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc
    26821 caatgtgcat acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt
    26881 gtaatgaaac aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat
    26941 ggatatgttg aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat
    27001 gccggaataa tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa
    27061 ttaatgaatg tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca
    27121 aatgaagttg gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt
    27181 gttttcatta aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc
    27241 gctgtatacc ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta
    27301 ctttatgtct gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt
    27361 tgacttcata atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt
    27421 ataaaataaa tcgtaattct attatagaga gttgtgatta taaaaagata gc
//
LOCUS       DAZKCC010000031        27463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000031 DAZKCC010000000
VERSION     DAZKCC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.031"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..1410
                     /locus_tag="ACG8VH_001385"
     CDS             241..1410
                     /locus_tag="ACG8VH_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9109949.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1883..2437
                     /locus_tag="ACG8VH_001386"
     CDS             1883..2437
                     /locus_tag="ACG8VH_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9109950.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2610..3047)
                     /locus_tag="ACG8VH_001387"
     CDS             complement(2610..3047)
                     /locus_tag="ACG8VH_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9109951.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3095..4042
                     /locus_tag="ACG8VH_001388"
                     /pseudo
     CDS             3095..4042
                     /locus_tag="ACG8VH_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4313..4748
                     /locus_tag="ACG8VH_001389"
                     /pseudo
     CDS             4313..4748
                     /locus_tag="ACG8VH_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4764..5597
                     /locus_tag="ACG8VH_001390"
     CDS             4764..5597
                     /locus_tag="ACG8VH_001390"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9109952.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5842..6867
                     /locus_tag="ACG8VH_001391"
     CDS             5842..6867
                     /locus_tag="ACG8VH_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9109953.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7005..7130
                     /locus_tag="ACG8VH_001392"
     CDS             7005..7130
                     /locus_tag="ACG8VH_001392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109954.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7155..8270
                     /locus_tag="ACG8VH_001393"
                     /pseudo
     CDS             7155..8270
                     /locus_tag="ACG8VH_001393"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8353..9249
                     /locus_tag="ACG8VH_001394"
                     /pseudo
     CDS             8353..9249
                     /locus_tag="ACG8VH_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9265..10089
                     /locus_tag="ACG8VH_001395"
     CDS             9265..10089
                     /locus_tag="ACG8VH_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109955.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10121..11431
                     /locus_tag="ACG8VH_001396"
     CDS             10121..11431
                     /locus_tag="ACG8VH_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFP9109956.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11579..12838
                     /locus_tag="ACG8VH_001397"
     CDS             11579..12838
                     /locus_tag="ACG8VH_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9109957.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12848..13720
                     /locus_tag="ACG8VH_001398"
     CDS             12848..13720
                     /locus_tag="ACG8VH_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109958.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13732..14562
                     /locus_tag="ACG8VH_001399"
     CDS             13732..14562
                     /locus_tag="ACG8VH_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9109959.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14602..16785
                     /locus_tag="ACG8VH_001400"
     CDS             14602..16785
                     /locus_tag="ACG8VH_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9109960.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16846..18066
                     /locus_tag="ACG8VH_001401"
     CDS             16846..18066
                     /locus_tag="ACG8VH_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9109961.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18063..19523
                     /gene="gtfA"
                     /locus_tag="ACG8VH_001402"
     CDS             18063..19523
                     /gene="gtfA"
                     /locus_tag="ACG8VH_001402"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFP9109962.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19600..20706
                     /locus_tag="ACG8VH_001403"
                     /pseudo
     CDS             19600..20706
                     /locus_tag="ACG8VH_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21028..21237
                     /locus_tag="ACG8VH_001404"
     CDS             21028..21237
                     /locus_tag="ACG8VH_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9109963.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21230..21379
                     /locus_tag="ACG8VH_001405"
     CDS             21230..21379
                     /locus_tag="ACG8VH_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9109964.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21533..22198
                     /locus_tag="ACG8VH_001406"
     CDS             21533..22198
                     /locus_tag="ACG8VH_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9109965.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22482..23567)
                     /gene="xerS"
                     /locus_tag="ACG8VH_001407"
     CDS             complement(22482..23567)
                     /gene="xerS"
                     /locus_tag="ACG8VH_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP9109966.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23901..24134
                     /locus_tag="ACG8VH_001408"
     CDS             23901..24134
                     /locus_tag="ACG8VH_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFP9109967.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24131..24538
                     /locus_tag="ACG8VH_001409"
     CDS             24131..24538
                     /locus_tag="ACG8VH_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9109968.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25675..26193)
                     /locus_tag="ACG8VH_001410"
     CDS             complement(25675..26193)
                     /locus_tag="ACG8VH_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFP9109969.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26560..26808
                     /locus_tag="ACG8VH_001411"
     CDS             26560..26808
                     /locus_tag="ACG8VH_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9109970.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            27311..>27463
                     /locus_tag="ACG8VH_001412"
     CDS             27311..>27463
                     /locus_tag="ACG8VH_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9109971.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         9405 a         4176 c         5075 g         8807 t
ORIGIN      
        1 tcttttatta ctatgtttgg gttttatagc aaagaaagtt taataagagt ttggacctag
       61 caaacaatat taatgaggtc attaaatctt attatcttta gtgatataac gaaatatcta
      121 cacagacaaa atgatagcgt aaaatatttt gtgtaaatag atatttaggg tagaaaaaaa
      181 gaaagtccat tctgtaaaat taaagttacc acaaccgaaa ttttatagga ggactttctc
      241 atgcccaatt ttactacaga aattatggaa acactaatca ataaaggtga tttagatgaa
      301 ttatttcgat gtcatttaga attagctgtt aattcattat tgcaggctga attaacagct
      361 tttcttgatt atgaaaaata tgatcgtgct gggttcaatt caggtaattc ccgtaatggg
      421 aattattcac gttcattcag aacagagtat ggagaattaa atttggtaat tcctagagat
      481 agaaatggta agttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
      541 gaaaccacta ttattcagct atttcaaaaa ggaattacta tgtctgaaat ctctgaattg
      601 attgaaaaaa tgtacgggca tcactatacg ccacaaacca tttcaaatat gactaaaata
      661 gtttctgaag atattattgc ctttaaagaa aggtccttag aatccagata ctctgtcatt
      721 tttatggatg ctactcacat tcctttaaaa agacaaactg tttcaaaaga agcagtttat
      781 attgtaattg gcattcgatt agatggaacc aaagaggttc ttgggtttag tcttgctcca
      841 actgaatctg cttatgtttg gaaagagatt cttcaagacc taaaagaccg tggtttaaaa
      901 gaggttttat tagtcgtaac tgatggttta agtggcatta acgatagtat tcatagtgtt
      961 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
     1021 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta tcaagcaagt
     1081 tcaaaagaag aagcaaataa tcaaataagg tttatgatag ataaatggaa aaagcagtat
     1141 ccacgagtgg ttaaattgct catgaatcct gctatattaa ccttttataa tttccctccg
     1201 tcaatcagaa gaactattta ttcaactaat ttgattgaag gttttaacaa acaattaaag
     1261 aaatatacca aaagaaaaga gcaatttcct aatgaagaat ctctggagag atttctagta
     1321 tctcaattca acaactataa tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa
     1381 atacatgata cattagaatc aatgttttaa cttgatcaac agaatgggct tttccattta
     1441 cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc gtaaagttat aaaatattaa
     1501 taaaaatatt ttattaatga aaagcttggt agcacggtta ttcatatgct aatttatttc
     1561 actttatagt aaggggagtt cttagtatat tgttcaaact taaatttcat ataatttaat
     1621 tatgtaaatt agctattcta agaacttcta cggataactg cccgtctaaa atttaaataa
     1681 gagcttagaa tcttcatata agtaaggtta atttttttgt acaaaaaagt cggaatattc
     1741 tgagttactg aaatgatata aaatcataaa ctaaatccct tgattttatt gactttcaaa
     1801 agtttatata tataagttaa tgattatggt aaaatatata cataatcatt aactcagaat
     1861 atttttaaga agggaaacaa tagtgagcta taatgtacaa ccgttaagga caaatgaaga
     1921 gatacaagat tttcagtttt ggctaagaag aacaagtaat ccggaaagag atagtttttt
     1981 atttttgttt ggaatcaata atgggttacg tatgtcagat attataggat taaaagttgg
     2041 agatatacga ggcaaaagta agcctattat tgttgaacat aaaacaggta agcgaaaacc
     2101 gatttttata gataatttga gagaagaaat tttattatat actgaaggta aagaagaaaa
     2161 tgattggtta tttccgagtc ggcaacaagg tcgacatata acaagggatc gtgtctatca
     2221 gatatatgca gatatagcag aaaaattagg acgagatgat ataggaacac atacattaag
     2281 aaaaactttt gggtatcact actacaagaa aactagagac attgctacct taatgtttat
     2341 ttttaatcat agctcgcaag caattacaaa aaggtacatt ggtataactg aagatgaaat
     2401 aggagcatca cttcgtggat ttaaactagg tgtataattt aaaaattttt ataaacagta
     2461 aagacaaaga ttaaaagcta tttttatcta gaacgatttt atttcataaa gttcgcggaa
     2521 taaaagctgg ggttactggt acttggtata ggttcgtgtt atataaacta agtaattaaa
     2581 taacattttt aggttggaaa aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa
     2641 acgcaaacgc tgcggaataa gaaaaatact gataactcaa cgtttagcag actatctaat
     2701 agggatagtc tgcttttttc gtttttgacc atacttttga ccttctgcaa acttccttgc
     2761 ttttcctctg tagaactcat gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt
     2821 ttcgcgatat gggtgtatat atcatccata gtggttttaa tatctgtatg tccaagtcga
     2881 acatacactt ctttgacaga cgcaccagat tcaaagagca gactacaatg ggtatgacga
     2941 aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa tgatgatttt caaattgtga
     3001 tttacatagt ctaaataaag gggcttattt tcttctgatg aaaacaaaag ttgttcacca
     3061 tcttttatag gtttgattct catttttaga tattatgccc gcacgtattc atcacatttc
     3121 tattgtgaat cgctttattc gaccgacatt tgacttttat cataatatac tcggtttaaa
     3181 attactaatg aaaacaatta atcaagacga tcataccatg tatcacctgt ttttttcaga
     3241 taatcaccac cgtgtgggaa ctgaactgac tttttttgaa gtacaagaag ggaacaacca
     3301 gttttttgga accaatacga ttgaacgaac gatactaaaa gttccttccg aagcatcatt
     3361 gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt
     3421 ttctggacga cctatcttac gttttgaagg gccagatgca acacgattag ctttagttcc
     3481 cttacgcgaa tttgaagata ttgatgatta tttcccgtat gtcacagacc agatccctac
     3541 agagcatgcc attttaggaa ttgatgccat tcaactacgc gtacaatatt ccgatgcgac
     3601 tgaacgagag ttattgtcgg taggctggta gcacaaagaa tacgtcagtt tcttttcggg
     3661 tcatcaacga gtgacaatct tagaaaatca agacactcag ttctatcaag aagttcagat
     3721 tattcaagat cgtgagaatc cagttgccat tgaagggatt ggtagcgtcc atcatgttgc
     3781 gtttggcgta gaaaataaat ctgatttaca acgcatcgac aaacaattac aagagcgcaa
     3841 ttttataaat tcaggaataa aagatcgtga attttttgta tcactttatt atcgtgaccc
     3901 aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga
     3961 aaatcaaagt ccacgctttg aagaaattcc tttattttta ccgcagtact taaatttcat
     4021 tcgtgaacaa gtggagcaat aattagctaa tcaaatccat gacgaagagt aagaggttga
     4081 ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat tctaaagaat gttgatttga
     4141 tgcggttcct atttttataa actatcattt tcgactaata aaactactta tgagtcagcc
     4201 tctctttttt tcttgatttt tatacctctt tctaggtaaa ctaaacatct gccaaaaata
     4261 ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa ggggagtacc aaatgaacga
     4321 gtatattgaa attatccacg caagagaaac aacttaaaaa tgtcagtctc aaaattccca
     4381 aaaataaaat tagtattttt accagcgtat ctggttcagg aaaatcatcg attgtttttg
     4441 atactattac ccaagaagct ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt
     4501 tatttttacc taaatacagt cgtccagagg ttgatgagat tgataatctt tctacggcga
     4561 ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc aacgttaggg acgattacta
     4621 acatcaatgc gttatttcgt gtgttgtttt catgttttgc aacacctagt ttaggatatg
     4681 caaatgctta ttcatttaat gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta
     4741 agacgattgc atttttaata ggattgcttt atttttcggc tattgataat gctgattttg
     4801 tattttctga tccatttacc gcagaagttt tgtctggaat tgaggaacag gcaaagaatc
     4861 atggttattt tgtattggtg cataatgtta attcagcaga agatgttaaa actattcaga
     4921 gaaattggcg ttttgagggg ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat
     4981 taaataacgt ttttgatggt cctgttgttt atattgatac gcatctttca aaagagacat
     5041 taagagaact tgaacaacaa atgaatcgtt tatttgtaaa tacaaatgat taccaagcta
     5101 gtacattggc agtggaacat ttgattcatt atggtcatga gaaaatagcg tttctttctt
     5161 ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg acatcaagct tatatcgata
     5221 cattaaagaa atatcattta acctttaaat cgcattggat atataacaac tctgaatttg
     5281 agcgaattta tcaagaatta gctgaattta ctgcaattgt tgttactgga gattatttag
     5341 cggctaaatt agtaaaattt ttaaaaaata agaataaacg agtgatggaa caattatcga
     5401 ttattggatt tgatgatatt acttttgcag aattaatgga tcctgcgttg acaacaatac
     5461 gactaaatcc aggtaacaag ggggcgattg cttttcacca actgattgga ctggccaaac
     5521 aagaagaaat aaaaaatcaa gtactactat tagagggaga attgatttct cgagactctg
     5581 ttcacaaaat caaataaaaa agctctgtga aataagtata gttaacaata aaatatccga
     5641 acgatagaga gaatgaactg tgatctagca gttcctcgtc gcaaattcac tagaattagc
     5701 attatttact ctaaattgtt cggatatttt ttcttgtttt taattatgat tacagtgcct
     5761 atttttaacg aaatttgctt attttcaaaa tgtatgatta tatgttagta aacactttca
     5821 aatatgaaaa gaggagctat tatggtagga attagggatg ttgcaaaacg ggcgggtgtg
     5881 tccattgcaa cagtatcgcg cgtgctaaat gaggatccga cactttcagt aaccgatgaa
     5941 accaaacgaa agattgcaga atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt
     6001 gcaaaaaatt taacaaatat tgcattaata acgacagttt ctgaagtaga tgagttggaa
     6061 gatccctatt ttcgaacgat tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc
     6121 aatttgaaga aaattattcg tttgtcagaa acaccgttgg agcaagacga tttgctaaaa
     6181 tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg tgattgaaga agtatcagca
     6241 attaattcga atgtcgtagt gattgatgac cctagtattg aagctgaagt agatgcagtt
     6301 tatactgatt taacaaaagc aacgaatcag catttagatc gattgtataa aaaaggacac
     6361 cgaaacattg cgtttatcgg aggaggacgt gttcgtttag atagccaagg aaataaacac
     6421 gtgtcagatg atgattatcg aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta
     6481 gaaaaatata gtcaaattta tttagaaggc tggaccacac ttgacggaat gcaggcagca
     6541 gatttacttt tgaaaaaaca tgctaaagat aagttgccga ctgctatcat ggtaggaaat
     6601 gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa atggtctaca tattccagaa
     6661 gatatttcga ttgtgagttt tgataatatt gaagttgctg aatttttaac acctgcatta
     6721 aatacggtga atgtgaatac agaagaaatt ggaaaactag cagttcgcat ggttaatgaa
     6781 cgaataaaag aagttagaaa tgtttcgatc aaagtgattg tttctaataa tattattgtt
     6841 agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt
     6901 tttgtttact aatattttta gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt
     6961 gttgttaaca aaaagaaaac gcattcaaaa caggaggaat taagatgaaa catttcaaaa
     7021 aaatagcttt aacagggata gctgttttaa ctgggacatt actcgcagca tgtggtgggg
     7081 gagatagtaa ttctaaagac gggcaagtaa ctttaagcta tgcgagttga gatactgacc
     7141 aagctccagg actaagaaaa atgttagatg aatttgaaaa acaaaatcct gatatcaaag
     7201 tagaaatgga gacaacgcct tgggatcaat attgggttaa attagaagct gcaacaacgg
     7261 gaggtaatat gccagatgta gtaaccatgc actctagcga atcctataaa tatatgtcac
     7321 agaatgcttt gatgggactt gatgatgtta tcaaagaaaa tgatattgac atgggaaact
     7381 ttactgaagg aattgctgat ttctatacgc ttgaagacga actatacgca atccctaaag
     7441 atgcttctgt agtggggcta tggtacaaca aagaattatt tgataatgca ggtgtcgatt
     7501 atccagatga aacatggaca tgggaaacac tcagacaagc tgcaattgat ttaacagatg
     7561 aatcaaaagg catctatggt tttgcatcac atattggaac agagactggc tactggccat
     7621 ttatttatca aaatggggga gaagtatttg ccgatgacaa tacaaaatca ggtttgaata
     7681 ccccagaagc acaagaagct ttagcttttt atcgagatat tgcacttgtt gatcatgctt
     7741 caccaacaat tacagaatta caagatcctg ataagttaag tcgttttcaa tcaggtagat
     7801 tagcaatgat gattgaagga aactggtttg caccgggatt tttgaaaaat gactatctag
     7861 ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt taatgcaaca attacaaatg
     7921 gacttggttg ggctgccagt gcgagtacaa aatacccaga agaagtgaaa aaactaatgg
     7981 catttttagc tagtgaggac gccaataaaa tccaagcgga aacgggggca tcaatcccag
     8041 cactaaaagg ctttgaagac gagtgggctg cttcttcaga tacaattgat ttgagtgctt
     8101 ttggaaaaat gttggagtat ggagtccctc gtccatacaa taaagatgga ttacgagctg
     8161 aacaagcaga agttcaaatt attaaccgta ttctgtctgg tgaagtaact gttgaagatg
     8221 gaaccgctca aattgaaaaa gaagtcaatg cgatattgag cggaaattaa attagttaat
     8281 tatacaatat acaggcgaaa ggattctatt tcgtctgtat attaatggat gaataccaaa
     8341 aagggggata ttatgaaaaa tcgaattttt ggaaaatatc gtaaagatca agtgatatgg
     8401 ggctatattt ttatttttcc aacggcatta ggattaattt tattaaatat atggccagca
     8461 attcaaacag caattttaag ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc
     8521 ggattaagaa attaccaaaa aatgtttgca gacggtgaag tttttcagtc tttattaaac
     8581 acattaattt atgcagtggt ttcgatacct agtattgttg tattatcctt gttggtggca
     8641 gttttaatga atagaaaagt taaaggattg tcgatttatc ggacaattta ttttctacca
     8701 gtcgttgctg caccatccgc agtcgcaatg atttggcgct ggatgttcaa taatgattat
     8761 ggactgatta ataacatgtt ggctaaaata ggtctcgatg gaccagcatg gttaacggat
     8821 ccatcgattg cgatttattc tattatcatt gtaggagttt ggagtgctat tggttataac
     8881 atggtgttat tactagccgg cttataagaa attcctaaag attattatga agcagcggaa
     8941 attgatggtg ctagtccaat ccagcagttt ttcaacatta cgttaccatt agttacacca
     9001 acattatttt ttgtagttgt aacaacagtt atcaatgctt tccaagtatt tgatgttatt
     9061 tttatgatga ttactcctaa tagtaccgcc atgtttaaat cacaatcatt agcctattta
     9121 ttctataaac attcatttat tttaaatgac aagggatatg gttcggcaat cgttatgttc
     9181 cttttagtga ttatattaat tattactgca attcaaatga agttgcagaa aaagtgggta
     9241 aactattaag gaggctatca aattatgaaa aataaatttt tttcaaatgc gccaatccat
     9301 atcatgctta ttttaggtgg tattgcaatg gcattacctt ttatttggat gattttaact
     9361 tcagggaaaa cgttggttga atctacccaa attccaccta caattttccc tgagaaattt
     9421 cagctaagta actatcaaca agtacttgat ttattaccat tcatggcttt ttatatcaat
     9481 acatttttaa tggttgttgg acgagtgatt ggctcggttt tattttcagc aatggctgca
     9541 tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc ttttcagctt agtacttatt
     9601 caaatgatga ttcccggttc catttttatt actccacagt atttattagc acaaaaattg
     9661 ggtattttaa atacaattcc agcattaata ttaccagggg tagttagtgc gtttggtacg
     9721 tttttattaa gacaattttt cttaggttta cctgatgaat tggaggaagc ggcctattta
     9781 gaaggtgcca atatttggac gatctttttc aaaattatgt tgccgcttgc taaatcagga
     9841 ttggttgcac ttggaatatt cacagcgttg tttgcattta aagatttaat gtggccattg
     9901 attgtcaata cttcaattga taagatgcct ttagctccgg gattagcaaa cttacaagga
     9961 caatatgcca cgaacttccc acaattaatg gcaggttcgt tattagcaat ttggccaata
    10021 gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag gaattgctac aacaggtagt
    10081 aaattataaa actattaatt agtgattgga gagtaagtac atgacaaaaa ttacatttat
    10141 tggtgcagga agtactattt ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag
    10201 tctacaagat gcacatattg cattgtacga tattgatgaa aaacgactaa aagaatcaaa
    10261 tttaatgctt aaaaatatta ataaaaattc gaatgaaaat cgtgctgata ttgtttctta
    10321 ttatgatcgc aaagaagcgt taagagaggc tgattttgtt atcaatgcta ttcaagtggg
    10381 tggttataaa ccttcaacag tgattgattt tgatattcct aaaaaatatg ggttgcgaca
    10441 aactattggt gacaccatgg ggattggtgg gatttttaga accctacgga cactaccagt
    10501 aatgttagac tttgctaaag aaatggaaga agtttgtcca aatgcttggt tattgaacta
    10561 tacaaatccg atggcgatgt taaccatggg tgttttaaaa gcgacgagta ttaagactgt
    10621 tggtttatgt catagcgttc aagtatgcgt accagaattg tttgagcatt taggaatagc
    10681 cgatcaatat gacttaaatg actttcaatg gaaaattgca ggcattaatc atatggcatt
    10741 cttgttggaa attacaaaaa atggggaaga tttctatcca acaattcgtc agttagctgc
    10801 agaaaaacca aatccacatc cagattctgt acgttttgaa ttgttgaaac gttttggtta
    10861 ctatattaca gaatcaagtg aacacaatgc agaataccat ccatacttta ttaaatcaaa
    10921 atacccagaa ttgattgacg aattagggat tccgttggat gaatacttac gtcgttgtga
    10981 acaacaaatt aatgattggg aaagcatgcg agaggacatt gtaaataatg gggagttaac
    11041 acatacgaga agccgtgaat atgcttcata tatcatggat tctattacga caggaactcc
    11101 gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc acaaacttac cgacagatgt
    11161 ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg caaccaacct atgtcggtga
    11221 attgccaaca caattagctg ctttaaatcg gacgaatatc aatgttcaag aactgacagt
    11281 agaagccgct ttaacactag ataaatcaaa aatttatcaa gcagcgtatt tagatccaca
    11341 tctatcatct gaattgtctt taacagatat caaaaattta gtggatgatt taatcgaagc
    11401 acatggggac tatttacctg aatataatta aaaagaagct gaaagagtaa ggaattttca
    11461 attccttact cttttcttag ttaaatttag taaaaatatt gacaaaacac ttagtaaaca
    11521 gttacattag tgttaacaaa aagaaaacgc attcaaattg tataaggagg agcataaaat
    11581 gaagtggtct aaaaaaatta ttttagcttg tatagcacta ggagcaacga tctctttgac
    11641 tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta gaaatagagt tttttaatca
    11701 aaaaaaagaa atgactcaaa cgattcagga gattgctaaa gattttgaag caaaaaaccc
    11761 tgatattcat gtaaaagttg tggatgtacc gaatgctggt gaagtaatta aaacacgtat
    11821 gcttgcaggc gatgtaccag atgtcattaa tctttatcct caaagtatag aattgaaaga
    11881 atgggcgaaa gctggttatt tagaggactt aaccgaagaa ccgtatttag agaatatcaa
    11941 aaatggatat gcacaacgtt ttgctattga ggatcgtgta tatagcatac cattaaccgc
    12001 taatgtctat ggtttttact ataacaaaac tgcttttgag gaaatgggga ttcaagcacc
    12061 agaaacttgg gcagaatttg aaaaaatagt agctgagatt aaagatcaaa acaaagtacc
    12121 gtttgcgatt gcaggagcag aaggctggac tttaaatggt tatcaccaac tagctatagc
    12181 gactgctgct ggtggtgaag aggaagcgaa caatgtttgg cgtttctcag aagttaatgg
    12241 aattaatgct gaaagcaaag aaatgcaaaa agattttaat cgattagatt tgttaagaga
    12301 accaaaagct ttgcaaaaca actggcaagg tgccggttat aacgatacag tagttacttt
    12361 tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg gcaatgccga tgattcattc
    12421 acaaaatcca gattttgaag taggaacttt tccttttcca gcagatgaag caggtcaaag
    12481 tctaacgatt ggcgcaggag acctagcctt atcagtttct gcaacttctc aacataaaga
    12541 agaagccaaa cgttttgtag aatatatgac aacacctgaa gcgatgcaga aatattatga
    12601 tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag aatactcagg attcaccatt
    12661 aagtggttta actgaacttg cttttactga tcgtcattta gtttggttag ccaaagattg
    12721 gaatagtgaa aatgattttt ataccttaac aacaaattat ttacacaatg gcaatcaaaa
    12781 agccatgatt aacgcattaa atgctttctt taatccaatg aaagctgacg gaaggtagga
    12841 aaataaaatg aaaataaaag aaattttaaa tcgttactgg gcaatcattt ttgtagcaat
    12901 tccaattatt ttgcagatga tttttttcta ctttccaatg gttcaaggtg ctttttacag
    12961 tttaactaat tggactggtc tgacttataa ttttgatttt gtgggtttga acaattataa
    13021 gattcttttt tctgatccga agtttatcaa atcgattggc ttcactttaa ttgtgacgct
    13081 ttgcatgatt gctggacaaa ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa
    13141 tattaaaggg aaaacattct ttagagcatg gttctttttt ccagcggtct tatcgggcgt
    13201 ggttgtttct ttagtattta aacaaatttt taactatggt ttaccaaaaa ttggtagtat
    13261 gttaggcatt gaatttttac aaaccagtct tttaggaaca acatggggag cgatttttgc
    13321 agtaattttt gttttacttt ggcaaggaat tgccatgcca gtcatcattt tcttagcggg
    13381 ttttcaaagt attccacaag aaattattga agctgctaga atggatgggg cgaatgataa
    13441 gcaaattttt tggaatattg aaatcaccta cttattacca agtatttcaa tggtatttat
    13501 tatggcatta aaaggtggct taacagcatt tgatcaaatt tttgctttaa ctggtggtgg
    13561 accaagcgat gccacaacat ctttggggtt acttgtttat aattatgctt ttaaaaacaa
    13621 tcagtttggt tatgcgaatg ctattgcttt agtcttattt atcttaattg ggattgtttc
    13681 actggcacaa ttaaaattat cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca
    13741 tcaaaagaaa aaaatatttc taaatacttg cttttattat tagggggctt catgatttta
    13801 gttcccttag tttcaacgct attcggttca tttaaaacaa cacccgatat tatgaataat
    13861 ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc gaagattgtt agcggatgga
    13921 attggtcatt acttttggaa ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca
    13981 gcaattattc ctatggctgc ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac
    14041 ggattgatgt atttattttt aattttagga atttttgttc ctttccaagt aattatgatt
    14101 ccgattaccg ttatgatgag taaaattggt ttagcaaata tgtggggatt aatcttactc
    14161 tatctcactt atgccgtacc acagacacta tttttatatg ttggttttat taaactaaat
    14221 gtgcctatca gtctggatga ggctgctgaa attgatgggg ctagtcactt tacaacttat
    14281 ttcaaaattg ttttcccaat gttaaaacca atgcatgcga cgactattat tcttaatgct
    14341 ctatggtttt ggaatgattt catgctacca ttattaattt taaatcgtga ttctaatatg
    14401 tggacactac cattgtttca atataattat acgggacaat attttaacga ctatggtcca
    14461 agttttgctt cctatacagt aggaattgtc acgattacac tagtttatct tgctttccaa
    14521 agaagtatta tagctggaat gagcaatgga gcagtgaaat aaccaataaa ttattctcat
    14581 tataaaggag ttcataaact tatgattata tttgacaatc acacacaaac atttcattta
    14641 caaaataaaa aaattagcta tctattagga atcgaagaaa ataattattt aacgcatctt
    14701 tattttggaa aaaagattac gcactattct ggtgggtatc gctacccccg aacagatcgt
    14761 tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat tatctttaga cacattacca
    14821 ttagaatttc caggatatgg acatggtgat tttagagaac cggcgcataa tattaaatta
    14881 ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct acgaaattca aaaaggaaag
    14941 cccagcttag cgggattgcc agccttatat acggaagatg atgatgaagc tgaaacgctg
    15001 attattcaat tgaaagacgc gattagtgga ttgactttac agttgttcta cacaatttat
    15061 caaaatcaca gtgtaattat tcgttctacg caattagtaa ataatagtga tgaaacagtg
    15121 gagattaacc gcttagctag tcaatcaatt gattttccaa atcgtccact cgagttaatt
    15181 catttaaatg gtacctgggc aagagaacgt caaatgacta gagaaaaaat tcatacaggg
    15241 ataaaagtat tagatagtaa acgcggatca agcagccatc aacaaaatcc gtttgttgct
    15301 ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat atggatttaa tttagtttat
    15361 agcggtaatc acgaaacggt gattcaaaaa gatccatttg accaaacacg ggtcattatg
    15421 ggaattaatt catttaattt tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca
    15481 gaagtcgtga tggtttatag taatgaaggt ctgaatacgt tatcacagac gtatcatgaa
    15541 ttgtataatc accgtttaat tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc
    15601 aacaactggg aagcaacgta ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat
    15661 gatgcgaaga acttgggcat tgaaatgttt gttttagatg atggttggtt tggcggtcgt
    15721 gaaaatgact tcacttcatt aggtgattgg gttgaaacag aaggcaaact tgcttgtggt
    15781 ttggaaaatt tagccaagca agtgcatgat aacgggatga aatttgggat ttggtttgaa
    15841 ccggaaatga tttctgaaga tagtgaacta tttcgagcac atccagattg ggcattgggt
    15901 attccaggtc gtgaccgctc aattagtcga aatcaatatg ttcttgattt ttcaagacaa
    15961 gatgttcgtg ataatatcta tcaacaaatg acggcgattt ttgatcgtgt accgattgat
    16021 tatgtgaagt gggatatgaa tcgaaatatg acagaagtct attctgtctt gttggactca
    16081 acgatgcaag gtgaagtggc tcatcgttat atgttagggt tatatgaatt tttagaaaag
    16141 ttaacaacga attatccaac tattttattt gaaagttgtt cgggtggagg cggtcgtttt
    16201 gatgcaggtt tcttgtatta catgccacaa acttggacga gcgacaatac cgatgccgtg
    16261 gctcgtctga aaattcaata tggaaccagc ttagtgtatc caatttcaag tatgggtgcg
    16321 catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc
    16381 aatgctgcga tggctggtgt ttttggttat gagctagatc taacgcaatt aacttctgaa
    16441 gaaaaagcag aaattaaaga acaagttgcc ttttataaaa agcatcgcca acttttgcaa
    16501 tatggaactt tttatcgctt ggaaagtccg tttgaaggca atgatactgc atggatgttt
    16561 gtctcaaaag accaaacaga agccatggtc ttttatttcc gagtattagc agaagctgcg
    16621 catccattag tcacattgaa attagcagga ttagcagaag aagcaactta tcaaatggaa
    16681 gatgtaacat ttgctggcga tgaattgatg aatctcggtt tttatattga ctcagaacta
    16741 catggtgatt atgcgacaca acgcttttat ttgaaacaga tatagtagct tgaatttaca
    16801 atataagtgc aacaccaaaa gagactcatt ccaaacgaag aaaatatgtt ttatttacac
    16861 gcatttacta aaaagcagcc tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc
    16921 tatgcaatga ttaactattg gttagataaa ggactaggcg gatttagaat cgatgcgata
    16981 ttaaacatca aaaaaaaact agagtatggc atctttgaat ctgatggaga agatgggtta
    17041 gccttcattg ggccctggat tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg
    17101 agagagcgga catttaaacc acataatagt atgacggtag cagaagccga tgttcctaat
    17161 gaacgtttag atgaatatat tggtgaagat ggctattatt cgatggtttt tgattttagt
    17221 tatacggata ttgatgtacc tgaaactggt gaatggttta aagatagcca atggacatgg
    17281 acggatatgc gtaccaatat ttttacgaat caattagtta cgcaagataa aggttggggc
    17341 gccttatatt tagaaaacca cgatcaacca cgttcaatta ataaatatat tccagaagaa
    17401 tggatcaatg attatagtaa aaaaatgttg gcgacgcttt ttatgttact aagaggtacc
    17461 ccttttattt atcaaggaca agaactagga atgaccaaca tagaaatggc atcattagaa
    17521 gattttgatg atgtagctac gcattctcag tataaacggg cattagaata tggattggca
    17581 ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg ataattctcg cacacctatg
    17641 caatggaata cgcaaaaaaa tgccagattc tcaacttctg ataatgtatg gttaaaagta
    17701 aatcctaatt atccagaact caatgcagaa gctcagttaa ccaatcaaca gtcggtattc
    17761 aattattatc atcaattaat taaattaaga aaaaatagtg tatacaaaga cgtgctaatc
    17821 tacggacaat ttctaccagt gaaagataaa aatgaacata ttttgcttta tgaacgccaa
    17881 ttagaagata aaatcgtatt agttatctta aacgttacag caacagaaca agaatattat
    17941 gtagatgaac agtaccaaca agtattaatc aataattatg aagatgtttt attagatcaa
    18001 agtcgaaaat tgcgtttacg accgtttgaa agtattgtat tagctaatta tggaggacaa
    18061 ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc agatagttta ggaaaaaaca
    18121 tgggcgaatt aaaacaagtc ttagacaaac atttacaagg tgttattggt ggtgtacatc
    18181 tgttgccttt ttttccatcg acaggtgatc gaggctttgc gccgagtgat tatacgacag
    18241 ttgatccagc tcttggaaca tgggaagatg tggaagcatt aggtgaagat tattatttga
    18301 tgtttgattt tatgattaat cacatttctc gtgaatcaaa atttttccaa gatttcaaaa
    18361 aaaaccatga gcagtcacca tacaaagaaa tgtttatccg tattcatgaa tttttcccag
    18421 aaaatcgacc aacacaagaa gatattgatt taatctacaa gcgtaaagac aaagcgccgt
    18481 ttcaaacggt agaatttgcg gatggtacga cagaagaggt ttggaatacc tttggggaag
    18541 aacaaattga tttagatgtg accaaagaag ttgtgaaaga atttattcga gaaaccatca
    18601 aagatatggc gaaccatggc tgttctttaa ttcgattaga tgcgtttgct tatgcgatta
    18661 aaaaattaga taccaatgat ttcttcgtgg aaccagatat ttgggaactt ttagacgaag
    18721 tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc agaaatccat gagcattatt
    18781 ccattcaaat gaaaattgcc aatcatgatt attatatcta tgacttcgcc ttgccaatgg
    18841 tcactctgta ttcactttat agtggaaaat cagaacgttt agccaattgg ttgaaaatga
    18901 gtccgatgaa gcagtttaca acattggata cgcatgatgg aattggtgtt gtggacgctc
    18961 gtgatttatt aacagatgaa gaattggatt acacctctga ggaattgtat aaagtcgggg
    19021 cgaatgtgaa gaaggtctat tcaagtgcca attacaataa tttagatatt tatcaaatta
    19081 acagcaccta ttatagtgca ttaggcgata atgaccgtag ctatctgtta gcacgtgcga
    19141 ttcaagtatt tgcgccaggg attccacaaa tttattacgt tgggttatta gcaggaaaaa
    19201 atgatattga actgttggaa gcaacgaaag aagggcgtaa tattaatcgt cattactatg
    19261 atttagccga aattgaagaa gaagtgcaac ggccagtgat tcaacaatta tttgcacttt
    19321 taaaatttag aaatgaatct gcggcgtttg atttggatgg aacgattgaa gtagcaacgc
    19381 catcagaatc agaaatagtg attacacgta aaaataaaaa cggtagtgat agtgcaacat
    19441 taacggctaa cttggatacg aaagaattta tcattgagtc taaagatata aaaattttca
    19501 ctagtgttgc attaaaattt taacctattc catagaacaa aaaatccttt atactagcac
    19561 ttggatgact tatccaagac caataaaaag gactcataca tggataagta taaaacaaat
    19621 acagcattta ataaatggtt ttctgccata aaattaggaa aactttctgt caactcccaa
    19681 aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag
    19741 ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt
    19801 atcgaccgat cgctccaact agaaaccgat gtgtcatcga ttagtcatac ccaattgtct
    19861 cgagcattta atgcacttga acctaaagtg ttagaagaaa tttttcaaca actgttagaa
    19921 aaagttcaac atcaaatcaa accaaccaag cgaaatcatc tgtatctgat tgattccagt
    19981 acgttttcgc ttagtttaaa acgtcataag tgggcgactt tccgtcaaac aaaatcaggt
    20041 gtcaagttac acttaaacta ttgttatatg gacgatacta cgatgtatcc aactgatttt
    20101 accttaacaa atgcccatga acatgatctc aatcaactac cagtattggt gaatcaacca
    20161 gaggcaactt acgtgtttga tcgtgggtat taggactttg aaaagatgga tcagatgcat
    20221 tgggatggct atttcttcgt cactcggatt aaaaaaaaca ccaaagttca cgtgatagat
    20281 actctggaaa catcgccaga aacagaaatt ctacgagacg agctggttcg tttaggatct
    20341 aaaacctatc taaccgccaa tttccgacta gtcactgttc aagataagaa cggacgtgtg
    20401 tttcaattta tcaccaatcg aatggacgtc tcttcaaaag aaatctctga tatgtatcac
    20461 gctcgttggc aaatcgaatt gtttttcaag cacatcaaac aacacatgac cattaaaacc
    20521 ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga ttttaacgat gatttccgct
    20581 ctcctcacgt ttctcatcaa attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa
    20641 cgattctttc gttatttact gtttcagccc tttgaatgtt gtctagaaaa attaattccc
    20701 acatgagaaa aaagaaattt ggctcgttac ttttggtttt cagttgaata acagataaaa
    20761 aattttctgg aaaaagtcat cctctgcgtc agcacagttt tttttgactt attttcagag
    20821 gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat
    20881 tagatttcta atgtgcattt tttgagtatt aaggagcaag tatacaaaga tttataaagc
    20941 tgattaaatt tgaataaaaa tatgcaggaa cgctactatt tttagtagtg ttctttttta
    21001 tatttgaata aaaaatgtgg tggttaaatg gtggttataa caaaaaatga attaatgaaa
    21061 ttaggttatg ggaagtatca gtctgagaga atcatacgac aggcaaaagc aattatggtt
    21121 tcaaaaagct atacctatta cgaaaataga agattaggac tagtacctgt tgaatctgta
    21181 gaagaagttt taggtatgaa gatagaggta gaggaggatt ttcagaatca tggttaattt
    21241 taaaaaagat aaaaatggga aatatcattt tactgctagt ttgggattcg acaaacgaac
    21301 agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt
    21361 ataccgaact tgtttctaat tatggaaaag aagcagaaac ttctcaaagt actatgttgt
    21421 ttgaggagtt tttttataaa ttattcctac cttattatca agggaaagta gaagaaagaa
    21481 catataaaaa tcggttatct acaatcaaaa aaacttttct tatttcttta aaatggagtt
    21541 aagagaaata cgtccaattg acattcaaaa gtggcaaaat aatttattga gcaaatataa
    21601 aaattcttat gtaagaaaca tgtatgggat ttttcagatg tcattagatc gagcagttgt
    21661 tttgggacta cttgaaaaaa atcctgcaaa agttattgga aatgtgaaga aaaggaaaac
    21721 tacagttgat ttttggacaa aagaagaatt tacaaaagtt atttcaactt tttatattga
    21781 tgatttgtac caacattttg gttttatttg tatgtggtta ctttttatga ccgttatgcg
    21841 tttgggagaa gccactgcat taacttggaa agatattgat tttgaaaata aaaacttgcg
    21901 tgtgaataaa acactctatt ataagagagc caaaacctat gagtttgtag aacccgaaac
    21961 ggcttctagt attcgtacaa tcgaactaga cgacaataca attacctttt taaaagcttg
    22021 gaaggagcgt caagtttcaa taggaggcat gtcgtttgta ctttcttata atggaacacc
    22081 tactcagaaa tatacaatcc gtcatattat gagaagacat gcaacgatcg ttggtgttca
    22141 tataatccgc atacacggat taaggcatag tcatgcttct ttgcttatat cacaataaaa
    22201 agatgcactt cgaattaaag aaaggttaaa acatgctgat gtacaaacga ctttaggaac
    22261 ttatggtcat ttgtatctaa attcattgag agagataaca aatgatccca cagattatat
    22321 ccaaactaat ttttctaaaa tcaaaccgac acctatttac gataataaaa aataaatgat
    22381 aaacaaaagt aggatcacac caaaagaaac acactagaaa aactataata caggcttttc
    22441 tagcgtgttg tttcttattt ccgcctattt atacattata ctcataaagc atttaatgca
    22501 tcgatcgtag ccgcattgtc tatatgggta tataaagttg ttgtattggt acttgaatgt
    22561 cccaattgtt gggcagttaa taccaatgaa tttgtctgtt ggtagagtct agtagctaat
    22621 gtatgacgca gtttgtgagg gctgacttga attctaaagc cttttgaata ttttgctacc
    22681 atccgttcga ttgcaatacc ggatatacgc ttggcttctc cttggtaaat tgctagaaat
    22741 aaagcttttt cgttttctgt tactttatat cgttctttcc gaatagctag ataattagct
    22801 aaatattcca ttgcgaatgc tgctatattg acagcatctc ttttatttcc ttttctagtt
    22861 actaccacat tattcttatc taaagatagg tcatctaaat taatatttat aacctcggag
    22921 agtctcaatc cagatcctaa aatcagtgca ttgatcgcca ggtctctttc tacatctcgt
    22981 tgataataga tcaaagctcg tttactcaaa gtatttttat attcattggc aatataatct
    23041 agataatttt gtgtatcctt atctagaaat aattttttct caatggcatt ggcacgtgca
    23101 gctaacgtag ttttatctgt gatggttgct acttgatcca taggattatt gtctaagtat
    23161 ggttttccta gaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgcc
    23221 gtgatatttc tattgatagt agaagtagat agacctaagt tattattatc tttgagctta
    23281 gtttttgttg tctctttttt tacattttct tttgtgcgac gtttcaaatg agatttgtac
    23341 aagtttaggt cataggcagt taactgtctt atcgaggtga tagaagtatc aggaacgata
    23401 gactcactca gaagccattc ataaaagagc ttataatttt ttagatattc taaagtggtt
    23461 cgaggtgatt gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata
    23521 tgaagtatga ttgtatctat tttttgatat atttctgtat ttttcatgtc attcatcctt
    23581 tcaataagta ggggagaaga gtatctctat aattggacta taccaattat agaatgtttt
    23641 gtcaattggt atgctctaaa atttattaat ttgtttcaaa aaaagcatat aaacaggtat
    23701 acgtttaata atttaaaata aatggtatac ataaataggg aaggaaatag aagggagcaa
    23761 tctaaatttt ttgaattttc ttaattaatt atatagaaag aacaatcttt ctatagagtt
    23821 agacatgaga gggactatgc ttgatttaaa atcaacaagc gtatatactg tatatacaaa
    23881 gaaaaaggag ggagaagact atggcaatta aagaaagaaa atttagggaa gtggggaact
    23941 cagtagtatt aactatttca aaggagtttc ttaaagaaaa tgggttaaat cccggagata
    24001 cggttttttt agatgaagaa aaacttcgag cggcaattac aaaaaaacaa gccttatctg
    24061 atgaaattga tttagcaata tctcaagcat taactgaata tgatactact tttagagagc
    24121 ttgtcgataa atgagtattc attacctaga agccaaagat attataaaaa tgaatgtaat
    24181 ccaaataaaa aaatactctc ctaatgaacc ccttggtgta aaggatgcga atgctttaga
    24241 aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa gaggtttatc cttcgattta
    24301 tgaaaaagca gctatcctac ttatacaatt gataaagaaa catccttttc acaatgcaaa
    24361 taaacgtact gctttcttag ctacttttgt ctttttgaaa atcaatggac agcttttagc
    24421 tattgatcaa aaagatgctg taaatttagc ggtatatatt gctacgtatg ataaggattt
    24481 cgatcaacta aaatatgaag tgatcaacac tataaagaca catacgcagc ccatttaagg
    24541 agttactgta tgtgtcttta tagggttatt agctgctata gcgaaataat tgaagtaaat
    24601 tacaaatagc tataattcaa atccaactat atagaaaacg taaattttct atatagttgg
    24661 attaagataa cgactattta ttttatcata tttttactat accctatact tttacttggt
    24721 atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt agattaataa
    24781 aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa ttgatggtat
    24841 ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga cgattgctac
    24901 tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga aatgaaagac
    24961 cttttacatc ttgcagaaat cgttactggg attaaactaa gcaataaaaa ctaatgttcc
    25021 cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac gatttcacct
    25081 tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc agtaatagga
    25141 taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat ctgtatcgta
    25201 tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa gcgttgaatc
    25261 gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta ccggacttgc
    25321 cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg gtactatcgg
    25381 cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc ctaagtccct
    25441 ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt ttatctgttg
    25501 gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag gggagtgcac
    25561 ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg tatgttctta
    25621 ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg gaatttacaa
    25681 agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag caactgtatt
    25741 tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt ctgcccattt
    25801 tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca tgttaatatc
    25861 atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg cataggtatt
    25921 tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag aaggcgtttt
    25981 aacagataat ccatttcttt catcattttc ccattttaga attaaattcg tccaaccaac
    26041 ttgatatgct aaattttcag caggtgttct atccacttcc tcaactcttt tatccttcaa
    26101 ttcttcagga atagtttcaa attctaaaat atatttagta taagtcctat ttatttcttt
    26161 ttttaattct tctttatttt tatatgttct cattatatta cctccataaa ttcctaattg
    26221 tctcctttaa ttcatctata atttcatcca cgatatttta atccgttcta caatgcatat
    26281 ctgatatgca ttgactgtct aactcatatg tgaatgattt ataaaatatc ccaacgtctc
    26341 atctaaagaa atgttgatcg tcagcaagga gctaaaaaat acgaatatat ttcttttgta
    26401 gtaaaatcgg actggtttgc atatcatttt cttcgtaaat atcaaaatca tgttaactac
    26461 tatcaattac taataaaaac taccacttta agcgttaaga actgttaagt ttagttaagt
    26521 gaggatagtt cacaaaatcc attgttaaac ttcacgaaag tggtaaaact caaaatgctt
    26581 tatataggat tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa
    26641 gtaaaaattg acgatgacac gttccttacg gctcatcaga tgaaacagct acaaaaacga
    26701 aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat tgccatattc acgcctcgct
    26761 cgacgaacta ttaaaggtca agatttacct gaagatcatc ctttttagga aattgaagct
    26821 ttttatgttg cacaatgcat ttataatact acaatgatgt tttcaccaga tattcttcta
    26881 gggtgcggtg taatgaaaca gatacacttg caaaagaaaa ttcaccgcga atttactaaa
    26941 attatgaata attatgttaa tcttccgaaa tttttttcca aaattaaggg atcaagctgg
    27001 tgttatagat tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat tatagaaaac
    27061 cgatactctg ttaagttggt tgacacggtg tcgattttta tgactttctt attctgaaac
    27121 ttcactaacg gacagtacct aaaaagtcta tagtatggac gtttacgagt actattttat
    27181 caaaaatgct gctcatttca acttatatgt tttagtgaac tacccccact tagcttggat
    27241 aggctacaat taactagaca gaaaaattaa ggtgtgtaga ctagaagaaa acataccagg
    27301 aggaattttt atgtctaaga gaacacgaag aactttttca caagaattca agcaacaaat
    27361 cgtcaatctt tacttagctg gaaagccacg tgtagaaatc attcgagaat atgaactaac
    27421 ggcttcagca tttgacaaat gggtaaagca atctaaaacg agt
//
LOCUS       DAZKCC010000032        26956 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000032 DAZKCC010000000
VERSION     DAZKCC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.032"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8VH_001413"
     CDS             complement(164..388)
                     /locus_tag="ACG8VH_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFP9109972.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8VH_001414"
     CDS             complement(586..2166)
                     /locus_tag="ACG8VH_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9109973.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8VH_001415"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8VH_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9109974.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8VH_001416"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8VH_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9109975.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8VH_001417"
     CDS             5331..5765
                     /locus_tag="ACG8VH_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9109976.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8VH_001418"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8VH_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP9109977.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8VH_001419"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8VH_001419"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9109978.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8VH_001420"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8VH_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9109979.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8VH_001421"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8VH_001421"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9109980.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8VH_001422"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8VH_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP9109981.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8VH_001423"
     CDS             11001..11471
                     /locus_tag="ACG8VH_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFP9109982.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8VH_001424"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8VH_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9109983.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8VH_001425"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8VH_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9109984.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8VH_001426"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8VH_001426"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP9109985.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8VH_001427"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8VH_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP9109986.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8VH_001428"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8VH_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9109987.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8VH_001429"
     CDS             16164..16967
                     /locus_tag="ACG8VH_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP9109988.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8VH_001430"
     CDS             17145..18092
                     /locus_tag="ACG8VH_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9109989.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG8VH_001431"
     CDS             complement(18160..18453)
                     /locus_tag="ACG8VH_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP9109990.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG8VH_001432"
     CDS             complement(18481..19146)
                     /locus_tag="ACG8VH_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP9109991.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG8VH_001433"
     CDS             complement(19286..19918)
                     /locus_tag="ACG8VH_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9109992.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8VH_001434"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8VH_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP9109993.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8VH_001435"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8VH_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP9109994.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8VH_001436"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8VH_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9109995.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8VH_001437"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8VH_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP9109996.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACG8VH_001438"
     CDS             23883..25052
                     /locus_tag="ACG8VH_001438"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9109997.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25319..26953)
                     /locus_tag="ACG8VH_001439"
     CDS             complement(25319..26953)
                     /locus_tag="ACG8VH_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP9109998.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         8249 a         5325 c         4287 g         9095 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
    25381 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt
    25441 tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag
    25501 cattttttga attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg
    25561 ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac
    25621 tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt
    25681 tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt
    25741 cattagaata agttccttga aaattcattt ctttcaccat gttaactact gatgaataat
    25801 ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt gtataagaaa
    25861 atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaac aatgttgaaa
    25921 atatcataaa acgccaagga gcttgaataa tatttaaagg agtattttgt aaggcaaacc
    25981 atggaaatat ttttgtacaa agtagaatag atattatacc cattatataa gtgattttag
    26041 ataagattcc taatttttta aagttaaatg ttagtataat taaacaaata agcaaagtta
    26101 agccaatacc aaatgatagt aattctgaat ctatacttcc aatcaaaaac tctttaccca
    26161 gataaattgc tcttttttgt aatatatcca cataaggtct attcagatct tgatacaatg
    26221 tctgctcaat cataggacct ataaacgcta gtgaaagaag taaagtaact atagcagctt
    26281 ttattaattt aataattaag aagatttcta ttttcttttt ccgataaaat tgaataatta
    26341 aagcaattat aatcattacc atagtcatag ctacagaaag aacatgagtg taaattaata
    26401 aagtcatgct aatcgccaac ataacccaag attctttttt tgagtcgtaa agctttataa
    26461 acccgagtaa aataagagga agtattgaca ttgcgagtaa ttcccccatt gcccctcgaa
    26521 gaaatatttc tacactccta aaagaagatg ttgtatatat aatagcgaaa aaggagctag
    26581 taacatgttt tttggtaatt tcatacatta cataatgaca tataaataga gtaatgagcg
    26641 taacaatata aagaaaaagg ttatagccta ttgctaaatt agtaaacttg atcaataaaa
    26701 ataaaggata tagagttaac catgggtaaa aattatttac ttgcatgcca tatgatctaa
    26761 acgtttcaaa attaataggg gagctaaaaa tagattctaa acttaacgca cgattaatgt
    26821 gaaatgccgt atcataaaag ccaaatattt ccatgttttt aaatgctgga aataaatata
    26881 ataaactaac aaatgcataa actataaata tttttacaga aaaaattttt ttcaaattca
    26941 tataccacct catgtt
//
LOCUS       DAZKCC010000033        26796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000033 DAZKCC010000000
VERSION     DAZKCC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.033"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG8VH_001440"
     CDS             complement(104..1336)
                     /locus_tag="ACG8VH_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9109999.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG8VH_001441"
     CDS             1591..2160
                     /locus_tag="ACG8VH_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9110000.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG8VH_001442"
     CDS             complement(2338..2778)
                     /locus_tag="ACG8VH_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9110001.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG8VH_001443"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG8VH_001443"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9110002.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG8VH_001444"
     CDS             3732..4655
                     /locus_tag="ACG8VH_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP9110003.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG8VH_001445"
     CDS             4731..5870
                     /locus_tag="ACG8VH_001445"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP9110004.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG8VH_001446"
     CDS             5863..6663
                     /locus_tag="ACG8VH_001446"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9110005.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG8VH_001447"
     CDS             6663..7490
                     /locus_tag="ACG8VH_001447"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9110006.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG8VH_001448"
     CDS             7495..8202
                     /locus_tag="ACG8VH_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9110007.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8VH_001449"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8VH_001449"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9110008.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8VH_001450"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8VH_001450"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP9110009.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8VH_001451"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8VH_001451"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9110010.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8VH_001452"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8VH_001452"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9110011.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG8VH_001453"
     CDS             11787..13820
                     /locus_tag="ACG8VH_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110012.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG8VH_001454"
     CDS             13864..15144
                     /locus_tag="ACG8VH_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP9110013.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG8VH_001455"
     CDS             15354..16160
                     /locus_tag="ACG8VH_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9110014.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG8VH_001456"
     CDS             16172..17392
                     /locus_tag="ACG8VH_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110015.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG8VH_001457"
     CDS             17382..18968
                     /locus_tag="ACG8VH_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9110016.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG8VH_001458"
     CDS             19007..21145
                     /locus_tag="ACG8VH_001458"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9110017.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG8VH_001459"
     CDS             complement(21513..22496)
                     /locus_tag="ACG8VH_001459"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9110018.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG8VH_001460"
     CDS             22874..24265
                     /locus_tag="ACG8VH_001460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9110019.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG8VH_001461"
     CDS             24281..25321
                     /locus_tag="ACG8VH_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9110020.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="ACG8VH_001462"
     CDS             25340..26425
                     /locus_tag="ACG8VH_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP9110021.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26796
                     /locus_tag="ACG8VH_001463"
     CDS             26458..>26796
                     /locus_tag="ACG8VH_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9110022.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKN"
BASE COUNT         8597 a         4450 c         5439 g         8310 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa
    26761 gataatgagt atatttataa ttattttaaa aagaat
//
LOCUS       DAZKCC010000034        26660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000034 DAZKCC010000000
VERSION     DAZKCC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.034"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..729)
                     /locus_tag="ACG8VH_001464"
     CDS             complement(19..729)
                     /locus_tag="ACG8VH_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP9110023.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACG8VH_001465"
     CDS             complement(726..2198)
                     /locus_tag="ACG8VH_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9110024.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACG8VH_001466"
     CDS             2336..2809
                     /locus_tag="ACG8VH_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9110025.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACG8VH_001467"
     CDS             complement(2962..3513)
                     /locus_tag="ACG8VH_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9110026.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACG8VH_001468"
     CDS             3618..4148
                     /locus_tag="ACG8VH_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP9110027.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACG8VH_001469"
     CDS             complement(4631..5074)
                     /locus_tag="ACG8VH_001469"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9110028.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACG8VH_001470"
     CDS             complement(5090..6250)
                     /locus_tag="ACG8VH_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9110029.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACG8VH_001471"
     CDS             6757..8457
                     /locus_tag="ACG8VH_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110030.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACG8VH_001472"
     CDS             complement(8559..9392)
                     /locus_tag="ACG8VH_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9110031.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACG8VH_001473"
     CDS             complement(9696..11861)
                     /locus_tag="ACG8VH_001473"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP9110032.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACG8VH_001474"
     CDS             12121..14415
                     /locus_tag="ACG8VH_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP9110033.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACG8VH_001475"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACG8VH_001475"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9110034.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACG8VH_001476"
     CDS             15066..16073
                     /locus_tag="ACG8VH_001476"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9110035.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACG8VH_001477"
     CDS             16095..17114
                     /locus_tag="ACG8VH_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9110036.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACG8VH_001478"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACG8VH_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9110037.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACG8VH_001479"
     CDS             complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACG8VH_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP9110038.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACG8VH_001480"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACG8VH_001480"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9110039.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACG8VH_001481"
     CDS             complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACG8VH_001481"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9110040.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="ACG8VH_001482"
     CDS             24539..25840
                     /locus_tag="ACG8VH_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9110041.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="ACG8VH_001483"
     CDS             complement(25909..26508)
                     /locus_tag="ACG8VH_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9110042.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8098 a         5483 c         4772 g         8307 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta
//
LOCUS       DAZKCC010000035        26593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000035 DAZKCC010000000
VERSION     DAZKCC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.035"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG8VH_001484"
     CDS             172..516
                     /locus_tag="ACG8VH_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9110043.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG8VH_001485"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACG8VH_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACG8VH_001486"
     CDS             complement(1028..1378)
                     /locus_tag="ACG8VH_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110044.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACG8VH_001487"
     CDS             complement(1344..2675)
                     /locus_tag="ACG8VH_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFP9110045.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACG8VH_001488"
     CDS             2931..3497
                     /locus_tag="ACG8VH_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9110046.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACG8VH_001489"
     tRNA            3642..3729
                     /locus_tag="ACG8VH_001489"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ACG8VH_001490"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ACG8VH_001490"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9110047.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ACG8VH_001491"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ACG8VH_001491"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP9110048.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ACG8VH_001492"
     CDS             complement(7452..7898)
                     /locus_tag="ACG8VH_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9110049.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG8VH_001493"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG8VH_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9110050.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ACG8VH_001494"
     CDS             8363..8881
                     /locus_tag="ACG8VH_001494"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9110051.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ACG8VH_001495"
     CDS             complement(8958..9914)
                     /locus_tag="ACG8VH_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9110052.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ACG8VH_001496"
     CDS             10080..10886
                     /locus_tag="ACG8VH_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110053.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ACG8VH_001497"
     CDS             10883..11872
                     /locus_tag="ACG8VH_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9110054.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ACG8VH_001498"
     CDS             11869..12873
                     /locus_tag="ACG8VH_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9110055.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ACG8VH_001499"
     CDS             13005..13547
                     /locus_tag="ACG8VH_001499"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9110056.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ACG8VH_001500"
     CDS             13567..14265
                     /locus_tag="ACG8VH_001500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9110057.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ACG8VH_001501"
     CDS             14286..14498
                     /locus_tag="ACG8VH_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP9110058.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ACG8VH_001502"
     CDS             14498..15460
                     /locus_tag="ACG8VH_001502"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP9110059.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ACG8VH_001503"
     CDS             15478..15873
                     /locus_tag="ACG8VH_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9110060.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ACG8VH_001504"
     CDS             16036..16401
                     /locus_tag="ACG8VH_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9110061.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ACG8VH_001505"
     CDS             16398..18209
                     /locus_tag="ACG8VH_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFP9110062.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="ACG8VH_001506"
     CDS             complement(18271..18438)
                     /locus_tag="ACG8VH_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP9110063.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="ACG8VH_001507"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="ACG8VH_001507"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9110064.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="ACG8VH_001508"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="ACG8VH_001508"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP9110065.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="ACG8VH_001509"
     CDS             21195..22175
                     /locus_tag="ACG8VH_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9110066.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22366..23088
                     /gene="tsaB"
                     /locus_tag="ACG8VH_001510"
     CDS             22366..23088
                     /gene="tsaB"
                     /locus_tag="ACG8VH_001510"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9110067.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="ACG8VH_001511"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="ACG8VH_001511"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9110068.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="ACG8VH_001512"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="ACG8VH_001512"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9110069.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="ACG8VH_001513"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="ACG8VH_001513"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9110070.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25707..26408)
                     /locus_tag="ACG8VH_001514"
     CDS             complement(25707..26408)
                     /locus_tag="ACG8VH_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9110071.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8608 a         4543 c         5540 g         7902 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct gatatttatc
    25681 agggtcactg cttatttttg ttcgcgttat tctctcatag ctggagtagt agctgtctta
    25741 cagaaagtaa aacgcttttt gtcataatct aacagcccgg aatcacgtag ttgtttgatt
    25801 acttgtccag cagtttcacg tgtcgtgccg ctattcactg ctaattcttt taaggtgatc
    25861 ggataaggaa tggtgatatg accatctttt gttggtacgc ccaattcttc tttcaaaatg
    25921 aataaatttt gttttacccg gtcaaaggcg ctagaggtca atccgatttg gatacgcaat
    25981 tcatgtattt caactacttt agaaattttt tgtatcatat agatcaactg ttcgggattt
    26041 tttcgtgcta atttttcata gtaggaaaca ggaaggtaga atgtttcaat atcagtcatc
    26101 gcttgagctg tataagtgta ggtctcttct ccaaacattc ctgacatagg aaaaaggtta
    26161 tttgtattca catagtcata gtagtaataa gttgcacttt catcataacg ttctaaacga
    26221 acgagtcctt tggtcaagat atacaaccgt tgtctgggat caccatcgtc gaaaaggatc
    26281 tgtccttttt tataagaacg aagaacagta tgttccttga tctgttggaa ctcttcatcc
    26341 gagaaatgtt caaaatcagg ttgatatctg agttttgaaa agtgaaaatc gattttatta
    26401 acacgcatta aaaagtcctc tccttattgt tcactatatt tattgtcact ttttttaaag
    26461 aatttgtaaa gttttaacat accaacatta gaaacaagtt ttaatacttt acaaatataa
    26521 tttgttatta tgtaaaaaat atacaatggt attgaataat ttgttttgaa gcgaatgatt
    26581 tgtgtataaa aaa
//
LOCUS       DAZKCC010000036        25794 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000036 DAZKCC010000000
VERSION     DAZKCC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.036"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..519
                     /locus_tag="ACG8VH_001515"
     CDS             <1..519
                     /locus_tag="ACG8VH_001515"
                     /inference="COORDINATES: protein motif:HMM:NF028094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP9110072.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            512..1042
                     /locus_tag="ACG8VH_001516"
     CDS             512..1042
                     /locus_tag="ACG8VH_001516"
                     /inference="COORDINATES: protein motif:HMM:NF019106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFP9110073.1"
                     /translation="MNELWMDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIM
                     KPSDNGKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVEN
                     LEWLSSDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRH
                     TVLNIASGKTFSFLED"
     gene            1147..1977
                     /locus_tag="ACG8VH_001517"
     CDS             1147..1977
                     /locus_tag="ACG8VH_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFP9110074.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1994..2845
                     /locus_tag="ACG8VH_001518"
     CDS             1994..2845
                     /locus_tag="ACG8VH_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9110075.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2842..3201
                     /locus_tag="ACG8VH_001519"
     CDS             2842..3201
                     /locus_tag="ACG8VH_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFP9110076.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3216..3377
                     /locus_tag="ACG8VH_001520"
     CDS             3216..3377
                     /locus_tag="ACG8VH_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9110077.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3374..3679
                     /locus_tag="ACG8VH_001521"
     CDS             3374..3679
                     /locus_tag="ACG8VH_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110078.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3679..3996
                     /locus_tag="ACG8VH_001522"
     CDS             3679..3996
                     /locus_tag="ACG8VH_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP9110079.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            3993..4388
                     /locus_tag="ACG8VH_001523"
     CDS             3993..4388
                     /locus_tag="ACG8VH_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP9110080.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4385..4723
                     /locus_tag="ACG8VH_001524"
     CDS             4385..4723
                     /locus_tag="ACG8VH_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110081.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4720..5088
                     /locus_tag="ACG8VH_001525"
     CDS             4720..5088
                     /locus_tag="ACG8VH_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110082.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5085..5390
                     /locus_tag="ACG8VH_001526"
     CDS             5085..5390
                     /locus_tag="ACG8VH_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110083.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5414..5617
                     /locus_tag="ACG8VH_001527"
     CDS             5414..5617
                     /locus_tag="ACG8VH_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFP9110084.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5614..5910
                     /locus_tag="ACG8VH_001528"
     CDS             5614..5910
                     /locus_tag="ACG8VH_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110085.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            5986..6402
                     /locus_tag="ACG8VH_001529"
     CDS             5986..6402
                     /locus_tag="ACG8VH_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9110086.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7310..7654
                     /locus_tag="ACG8VH_001530"
     CDS             7310..7654
                     /locus_tag="ACG8VH_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9110087.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            7659..7940
                     /locus_tag="ACG8VH_001531"
     CDS             7659..7940
                     /locus_tag="ACG8VH_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110088.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            8043..8357
                     /locus_tag="ACG8VH_001532"
     CDS             8043..8357
                     /locus_tag="ACG8VH_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP9110089.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            8335..10029
                     /locus_tag="ACG8VH_001533"
     CDS             8335..10029
                     /locus_tag="ACG8VH_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9110090.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            10049..11227
                     /locus_tag="ACG8VH_001534"
     CDS             10049..11227
                     /locus_tag="ACG8VH_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9110091.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            11190..11876
                     /locus_tag="ACG8VH_001535"
     CDS             11190..11876
                     /locus_tag="ACG8VH_001535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9110092.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            11876..13036
                     /locus_tag="ACG8VH_001536"
     CDS             11876..13036
                     /locus_tag="ACG8VH_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9110093.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            13046..13921
                     /locus_tag="ACG8VH_001537"
     CDS             13046..13921
                     /locus_tag="ACG8VH_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110094.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            13918..14229
                     /locus_tag="ACG8VH_001538"
     CDS             13918..14229
                     /locus_tag="ACG8VH_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110095.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            14219..14572
                     /locus_tag="ACG8VH_001539"
     CDS             14219..14572
                     /locus_tag="ACG8VH_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9110096.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            14562..14963
                     /locus_tag="ACG8VH_001540"
     CDS             14562..14963
                     /locus_tag="ACG8VH_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110097.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            14956..15360
                     /locus_tag="ACG8VH_001541"
     CDS             14956..15360
                     /locus_tag="ACG8VH_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110098.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            15372..15980
                     /locus_tag="ACG8VH_001542"
     CDS             15372..15980
                     /locus_tag="ACG8VH_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9110099.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            16000..16362
                     /locus_tag="ACG8VH_001543"
     CDS             16000..16362
                     /locus_tag="ACG8VH_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110100.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            16365..16547
                     /locus_tag="ACG8VH_001544"
     CDS             16365..16547
                     /locus_tag="ACG8VH_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110101.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            16565..19885
                     /locus_tag="ACG8VH_001545"
     CDS             16565..19885
                     /locus_tag="ACG8VH_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9110102.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSIFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKIQNDNASAWR"
     gene            19936..20673
                     /locus_tag="ACG8VH_001546"
     CDS             19936..20673
                     /locus_tag="ACG8VH_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9110103.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            20683..22974
                     /locus_tag="ACG8VH_001547"
     CDS             20683..22974
                     /locus_tag="ACG8VH_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9110104.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPIRGTNSDLIIEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITAAQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHKTRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            22998..25088
                     /locus_tag="ACG8VH_001548"
     CDS             22998..25088
                     /locus_tag="ACG8VH_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110105.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSLDFGDYDYSGNPNLMANINADSFS
                     QGTGALSVIDDGDEVVITLDPNHKLDIYKMKSQPALVVGETYNMSIEIMLEDDFTGDP
                     SKIALRYFKMPNWVPALTTPNTLTTAKGVWQKLTVTAKMTTAIDNAESWYVTLYNQDA
                     NNSLSGRLRLRHAKLEAGSTATPYQPNLLVEPWSIGKVPLGDNIKDKGTVFPIKTSAY
                     AVYSGANTEPYIAGQKYTLTMKATKPATQTFNVFLDAGTIGVGSMTPVDGLTDVWQKT
                     FTVSQANIDAGVTNRLAIYQVPNSSVGSVQIDWLKIEKGDTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            25105..25254
                     /locus_tag="ACG8VH_001549"
     CDS             25105..25254
                     /locus_tag="ACG8VH_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110106.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     CEGNK"
     gene            25251..25697
                     /locus_tag="ACG8VH_001550"
     CDS             25251..25697
                     /locus_tag="ACG8VH_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110107.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            25699..>25794
                     /locus_tag="ACG8VH_001551"
     CDS             25699..>25794
                     /locus_tag="ACG8VH_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9110108.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         9246 a         3855 c         5561 g         7132 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac ttccgtttcg aaggtgtcac agggcgcaaa
      121 ataagcctca aagatgaatc gagcacaacc gtaagtgatg ccaatatctt agcgaatatc
      181 atcctagatt tcatctttga gcatgatatt ccgtttaaaa atggatatga aattttgccc
      241 caaaacgaac agtatttctt ttacaaatgc ctcacgaaca gggtgtgctg tagttgcgga
      301 aagaaaaatg ctgatataca tcacgtagac tctgtcggca tgggaaataa ccgaaaaaaa
      361 ataaacaatt ctggtaggcg ctttatggct ctgtgcagag aatgccatac gaaaattcat
      421 gtcgaaggtt ttactacatt tacaactaaa agaaagctta cggcagttgt tctcaaagac
      481 agtgatttaa aaagattggg gttgaatatc attgaatgaa ttatggatgg atatcgaagg
      541 atacgaggga gtatatcaag ttagtagctt aggaagagtt aaatctaatc gccctaacta
      601 tgtaattaat aagggatacg ttaacaaaga aggatttatt atgaaaccat ctgacaatgg
      661 gaaagggtat caaattatct tcttaagcgg caaaggtttt agagatagaa ggtatgtcca
      721 tcggctagta gcttttcact ttttaaaaga agcgtatttc gaaggtgctg aagtcaatca
      781 taagaacgga gataaatcta acaatactgt tgaaaaccta gaatggctca gttctgatga
      841 taataaaaag catgctagaa aaaatggatt aactaacttg aaaggaccat caaaactaac
      901 tgatttgcaa gctttagcta tcaagcgttt atatacaaac aaattgatga gctccggaga
      961 aataatgaaa ctttttaatg tcagcaggca taccgtgctg aatatagctt cagggaagac
     1021 atttagtttc ttggaagatt aagagaaaca caaaataggt gtgactaatt tcaaaaataa
     1081 atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg
     1141 ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa
     1201 atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta
     1261 ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct
     1321 tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg
     1381 ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc
     1441 agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct
     1501 gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac
     1561 gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat
     1621 aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa
     1681 agtaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca
     1741 gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg
     1801 atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg
     1861 agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag
     1921 caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa
     1981 ggagatgcat cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt
     2041 agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa
     2101 taaggatggt tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg
     2161 tgtgaaagag gatatacaga ccgaacggat atataacgat agcttaaaag cacgagcgtt
     2221 gagttttttt caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga
     2281 aaattatcac gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac
     2341 gaatgaggtt ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg
     2401 aaaaagccac ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa
     2461 gaaaatactt tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa
     2521 tactgaactg agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt
     2581 ggtggttttt gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt
     2641 tacaaacaac acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac
     2701 aacaaactta tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat
     2761 atttaagaat tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa
     2821 accagtgaaa ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt
     2881 atcaagtttg attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa
     2941 gcaaatggct ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta
     3001 gacggaaaga agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc
     3061 aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa aagactttaa gatcaaggca
     3121 aagttgttct gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg
     3181 ttcaaggaag agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa
     3241 tgcgtattcg aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg
     3301 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc
     3361 gtagattgga ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac
     3421 caagtcagaa tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     3481 atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg
     3541 tgatcgaatt ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa
     3601 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
     3661 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
     3721 atcatgatga agattcagaa agacaaaaaa tcaaaagaac gagcggaatt agttaagttg
     3781 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
     3841 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     3901 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca agtttgtaaa gaaatataaa
     3961 gagatattag acaaatacat ggaggacagc aaatgatact gaagtacagg gcatggaata
     4021 agaaaactca gtcatttatt gattatggtg atttggtttt agatttgaga agcggaaaaa
     4081 tttacgctgg ggacattggg ttagtggaaa gcaccattga tgtgactgac caaattgaac
     4141 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     4201 taattacatc aaaaggagcg attggatatg ttgcgtatct tcaacaagaa gccggctttg
     4261 tagttgtact aaagaaaagt gattatagat taggccatag gaatactggc gaaagttatg
     4321 atgtagcaac tgctcatgaa gtcatcggaa atatatacga gaatagcgag ttattggagg
     4381 tagagtgatt atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt
     4441 cccagcattg ctaaattgtg gaattaaaag caagaataga atcattttgt tacggatatc
     4501 ggctgattta gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac
     4561 acgttatcag tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc
     4621 tgacatgcat ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc
     4681 cgatgatttt ccaaatttat ggttgaagga ggaagcggaa tgaacgagga aataaaaaaa
     4741 tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat tgaaagtcat
     4801 aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt acgtaatgca
     4861 aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa acacagcgaa
     4921 ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga ggaaaatgga
     4981 tactacattg caactgagta tgacgatggc gattatgcag ataatggcta ttctgcttat
     5041 atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat gttcaagttt
     5101 atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc cacccatccg
     5161 aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca agagatgatg
     5221 cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg tatacggctg
     5281 taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa agacctaaga
     5341 aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga aaacaaaaca
     5401 gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
     5461 gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag aaacatataa
     5521 agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg gaatttcgat
     5581 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttacgct
     5641 agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc taaaacacgt
     5701 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg gttcatctat
     5761 ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg cattgaaaac
     5821 acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt ggtgagattg
     5881 gtggatattg aactaatcaa tgacaaataa aaaagccgga tcgctccgac taacataata
     5941 aaacagacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
     6001 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     6061 ttggagcgta tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     6121 ccgaaggcgc cgaagcacgg caataaggca gaggacgcga tcattcagat gatggatata
     6181 gaagcagaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     6241 atactctact acagcttctg cgacgtaaat aagcattcta attatgaaat aggtcaattg
     6301 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt gattgaattt
     6361 gctgaagcat ataaaaaagg cgtattaatt cagtatcgtt gattttgtag ggtttttgta
     6421 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
     6481 gaaacaggac ttcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
     6541 gcttcgatag acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa
     6601 attgttctta ttatctatca tccgttgctg tctattgtca ttatgtcact gtggcggaaa
     6661 gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt
     6721 gaccatgcaa tgttcgattc attgccagtg acttaaggaa cctacggaaa caattcatct
     6781 tatcggatgc cgatgaattg gctgactagt cgggatgcta ctagcagtta gaaggcataa
     6841 aatactagcg cagacgtgcg ccactctcag gtgtaggtta ggagagaaac attagttggg
     6901 gttattagga atacgataac ctgcttgcga caaagctttg tactgtcgcg ttggtcatga
     6961 acagagacgg tattctgttt cagtattcgt tagcaaccga gggatgtggc agtggtgagg
     7021 tgcaggaagt atcagacttg tttgtgtgca agttactatt acatattaga tcactctttg
     7081 agtggtcttt ttattttgcg taaaggaggc acgacgatgt atagaccaca atacttagaa
     7141 cagaagcatg aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg aaagtataga
     7201 agaccaataa agagcgatac atataaacgg aaggaaagca atgaagttat tccattgtat
     7261 ggcaaaagaa tagctaagta ttaaataaga ttgcgaaagg agatgggaca tgaccgagga
     7321 attctataga tggttattac agttgataag agaagatcgt ttggttaagt tctatcagtc
     7381 tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg aatgccaaga
     7441 gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa aggaagtcaa
     7501 ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg ttgaacatca
     7561 taatgaagtt catggcagat atcttacagc gttagataaa caagagaaga agatagaaag
     7621 cttcgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat gacaatggca
     7681 gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga ttaattaaac
     7741 gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac tgttgtagta
     7801 aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc gaaagaataa
     7861 aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc gctgaagctt
     7921 tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt gggggtgatg
     7981 aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta
     8041 aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa
     8101 taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt
     8161 atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta
     8221 gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa
     8281 tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat
     8341 caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc
     8401 aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga
     8461 gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac
     8521 tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga
     8581 tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat
     8641 ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt
     8701 aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat
     8761 gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg
     8821 aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct
     8881 agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa
     8941 gattattcaa tgataaatgt ggctacttct ggtggtggta aaattcgaga ttatagagaa
     9001 ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa
     9061 tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt
     9121 ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg
     9181 gaaaaagctt gtccaactat taattacaat gcagatttaa aacggaaaat gtttcaagaa
     9241 tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac
     9301 agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa
     9361 gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga
     9421 cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa
     9481 catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat
     9541 atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat
     9601 attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat
     9661 atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt
     9721 gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt
     9781 aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat
     9841 gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa
     9901 acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct
     9961 gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta
    10021 gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt
    10081 cacaaaaaaa gcaacggttc ctttagaaga atacttttgt aaattgcaag ttgattttgt
    10141 gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc
    10201 ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa
    10261 tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt
    10321 aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat
    10381 tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca
    10441 aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata
    10501 cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat
    10561 ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa
    10621 tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca
    10681 atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta
    10741 cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat
    10801 agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg
    10861 agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat
    10921 tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc
    10981 agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt
    11041 tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagagcg
    11101 cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa
    11161 tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa
    11221 agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct
    11281 ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag
    11341 aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga
    11401 acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg
    11461 ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga
    11521 tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg
    11581 accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca
    11641 cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt
    11701 tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta
    11761 aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa
    11821 ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta aaataatgac
    11881 agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga
    11941 tggtaacgaa gaatctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa
    12001 aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca
    12061 gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt
    12121 tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga
    12181 acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg
    12241 gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa
    12301 agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat
    12361 tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac
    12421 tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa
    12481 agatatgcca gtcggaatga tgcgtgacat gagcaaacaa actagcggag aatatgctga
    12541 aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg
    12601 actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc
    12661 tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac
    12721 tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca
    12781 gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg
    12841 tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg
    12901 catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt
    12961 attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac
    13021 tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg
    13081 gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat
    13141 cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg
    13201 tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa
    13261 accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt
    13321 ctatatcgat tcggatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa
    13381 agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag
    13441 ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga
    13501 atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta accgtcctaa
    13561 agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg
    13621 gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat
    13681 gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc
    13741 aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga
    13801 agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt
    13861 tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg
    13921 acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc
    13981 tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa
    14041 agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc
    14101 gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt
    14161 gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat
    14221 ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca
    14281 aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc
    14341 tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg
    14401 atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa
    14461 agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga
    14521 taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta
    14581 aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt
    14641 ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt
    14701 acttcattct cgaaacagga gaaatagaaa tgatcactga gccaaaatat agcgtgggtc
    14761 aaaatctata tctaactttc tattcagaaa atagagaaga tttaacagga gactcactag
    14821 atattatttc attgattcaa aatcgttcga ttcgttttca gagaatggat cccaatcatt
    14881 taaaactaga gaaccaagat cgctatatcg atcaattggt atttacgttt agacgattat
    14941 tgaagagtga ttgtcatggc taaaaatagt tgggagctaa aaataaatgg acatgatgaa
    15001 cttcttgtgc ggatggaacg ctattcaagc gagagcgaac gactgattaa cgaagcattg
    15061 aaatcaaaag gttcggctat tgcagtggat aggattacag aaaaaattcc tgtttctgaa
    15121 gcagatttaa gaagagggca ccaacacgca aaaaatagtc gtccacttaa gactcaatac
    15181 attaatttgg gtttcatcat tagacctaca agaaaatttg agtatttaaa atatcctgat
    15241 ttggggatag gtacttctaa aagaaatcag ccggacgaat ttatgagaag aggattaggt
    15301 cttgcacttg atccaattac agaacttctg attcgtcaat tcgataaatt aaataaatag
    15361 ggggaacaac aatggctaaa acaaaaactg tagtaacaac gttcgataac ataagtatca
    15421 aacgaattgc ttttaatttt aagaacgcag gaaatgcaat cgtaacgaat tgtaatggac
    15481 aattagatgg cgaaacagaa atgcaaacgg tggttaaaaa atgtggagcg acagaagtaa
    15541 aatcaaaatc taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag
    15601 tttatcgacg tttcaatggg ttgaaacaag atggacgtat taaaccaggc atttactctt
    15661 acggtcctga ttccgtaggc gaagatttct cacttgctgc agagatcgtg gatgacttcg
    15721 aagaaaatag aaagttagtt ggtatgttag catgcacttc gaatacagga ttaacattct
    15781 ctattgaaaa tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag
    15841 atgaatttgg taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag
    15901 atcaatggat gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta
    15961 cggccactca atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa
    16021 attgaactaa acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta
    16081 ttgatgatca atagagattt taacacagac gagtttgcaa aaatgtcgat gggaaaaggc
    16141 tctatggata ttacagttat tcaaggtgca aaagccgtat atgtagctta tcgtcaagcg
    16201 aacatggtcg attacatttc attcgatgaa tttatagata aatgggattt tgatatggag
    16261 gttgcagtag ctgtatacag tactatgatg ttcaaacaag cacgtgatgc ctatcaaaaa
    16321 gaattcgaaa aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag
    16381 ttgaaacgtg ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt
    16441 tggttttaag cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagcgctt
    16501 atgaaggatg gaagaactat gcaataaatc aatcccagaa aaactaaagg aaaggaggta
    16561 aaaaatggct aaaaagagaa cggaagcaga agtaactttc atagctaacg atgacggatt
    16621 gaaatctacg ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact
    16681 agaacaagct caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc
    16741 ttctttagaa aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gtcaacgttt
    16801 agccaatgcc aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga
    16861 actgattaat caacaaacag ctcagcaaag gttatctaat gatatagata aaacgagtaa
    16921 ggcattggct caatctaaag gtgaatttaa aacgtatgag tcaactatga aagacttaga
    16981 taatgagcaa caacatctaa agtcaagcgc atcattagta gaatccgaat ataaaaaatg
    17041 gcaagcaact gctggccagt ctgctacaga atctgaaaaa ttagcaaaag cacaagaata
    17101 tgtcggcaaa cagagtgata ttgctgaaca aaaaatcgaa gtgctaaaaa aacaattaga
    17161 tgccacacaa aaagaatttg gagaaacatc taccgaagct ttagaaatga aaactaagct
    17221 taatgatgcg gaaagagaat tcgaagaatt aagtaatgct gctaaaaacg ttgatacctc
    17281 cacagtagat gatatcggca aaaaacttga tatgggtaat ttaatggaag ctactgatca
    17341 cttgtcagtg atcggagata agcttattga tgtaggtagt aagtctattg aagcagctgg
    17401 aaaagcacaa gctatgcagg cccaatttaa acaagtcttt ggctctttag aaggggaagc
    17461 acaggacgcc gttgagggaa tggctgaaga atttggaatg ttaccaaata cgatcaagcc
    17521 tgtttttata caatatacgt caatgtttaa aggacttgga tatgatacca aagaagctat
    17581 ggagttagct ggtgatagta ctcagttagc agcagatgca gcagcttttt acgataagtc
    17641 tatggatgat gctagtgaat ctcttaattc atttataaaa gggaactacg aaggtgggga
    17701 gcaaataggt ttatttgcta atgatactca aatggcagct tatgctgtta agcataattt
    17761 gataccagcg actgaaggag caaaaaaagc cagtgaagag tcattgttag ctgttgaaaa
    17821 agcacaatct aagtatgctg atgctattaa gaaacatggt gaaggatctt tagaagcaag
    17881 agaggctgct ttaaaactta aagatgcgca agataaaata aatgaagaat taggcccaca
    17941 aacgcaaaaa tggtctgatt tagatgaggc taccaagcaa gcagttcgag ttcaatatgc
    18001 tgaagatatg caaaaattag ccggtgctac aggtcaggct agtagggagt ctgatggttt
    18061 agaaaaccaa atgacaagag caaagcaagc gttagaagat ttttatgcct cattgggtga
    18121 agatatactg cctgtgttta tcaaagggtt gcaagcagga gcgaaagctt tgcaaggatt
    18181 agccgaatgg tggagtaaac ttgatgggcc aatgaaaaat ttcattttag ctcttggagg
    18241 aattctagca ttattaagca cattagctcc tgttataacc gcagttgtta cgatagttgg
    18301 cacatttggt tctacagttt tgctgccaat aataggaatt atagcgggcg ttgcagctgt
    18361 aatagcgatt gtaattacag cgtttcaaaa ctggggcgca atcactgact ggtttagtga
    18421 tttatggaaa aaatttaccg attggttagg tgatacctgg gaaagtataa aagaaagtgc
    18481 ctcatcagtt tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga
    18541 aatttggcaa ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc
    18601 ttccaacatc tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa
    18661 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
    18721 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
    18781 tatctttgat gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat
    18841 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa
    18901 tggaattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
    18961 atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc
    19021 tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
    19081 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
    19141 ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc
    19201 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg
    19261 gaacggtatc aaaaacactg ctttgaatct ttggaatgga attacaagca ctattagctc
    19321 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
    19381 ttccgacata ttcaataatg ttaaaagtac aattgctaac atttgggaag gtatcaaaaa
    19441 gactgttagc gctccaattg attggatcag agataaaatc agtagtatct ttgataattt
    19501 gaatatttct ataccacata ttccgttacc acattttaaa ttgagcggag aattcaatcc
    19561 attgaagggg aaaatcccaa cgttgggtgt tgattggtat gcgaaaggta gtgtgtttaa
    19621 ttctccgaat attatcggtg tcggcgaagc aggacctgaa gcagttttac ctttgaaaag
    19681 atctgtgctg caagaaattg gtgatcgtat cttgagtagc acatcagttt catctagggc
    19741 acaaacgatt caacctgtga ataactacga attcaatttc acaattgatg gtaacgcaga
    19801 tgaggttact atgaagcaaa caactcaaca aatcattgat agcattacaa aaattcaaaa
    19861 tgataatgct tcggcatggc gttaaacagg agagtatttc tcctgttttt ttagtattaa
    19921 aaaggatgtg aaaaaatgac tgattgtata cattctataa tcgatggatt tcctgattat
    19981 ttgcataaat tggctttagc tgaaagacca accatacctt ctccaaaaag acagagagtt
    20041 gaaacttctg ttttaggtag gttaggtggc ttagtacaag attactcgtt tgaagatatg
    20101 tcgtttacat tgcactataa ctatttagag gatgtggaag accatcaagc gttcaagcaa
    20161 tcgttttata tcatgcgtca ttggttaaac tatgcaaaga aattagaatt ctctgatgat
    20221 cccaacgtct attatgttat ccagactatc gatattgggg atgcagaaaa cgatattgtt
    20281 gaatggggag agttcgatgt aaatattact gcgaaaccat tcgcaagagt tcaagaagat
    20341 gtacctataa ccgtagataa accacagtca tttaacttgc tgaataatag tttagaagaa
    20401 agttttccaa agattatcat cactccttca gctacttcat gtcagttcat cttaaatgat
    20461 tatgtgttta gttttgaagg cttagtagta ggaactgaca tagtcattga tagtgatcta
    20521 atgctttgct acgaagagca atcggacgga gatattttag atcggtccaa caaaatgaag
    20581 accatgcaat atccgacatt gcaagtggat attaatcatt ttaattgcac tggtttgagc
    20641 aaaatacaaa tttatcgtaa cgggttaagg taggtgaaat agatgatcga taatttaata
    20701 actatttacg ataaaaacga cgcgaataat ttagctgaac atttatatga tacgcaaggt
    20761 ttaggcgctt tgtcagactg gttaacagcc actgttagca ataaactaaa cggagccgag
    20821 atatttcagg gtacttatcc aataagagga actaattcag acttgattat agaaggacgt
    20881 attattcagt gctatgtaga tgaaaatcga gcaaagcaac gtttacggat ttattatgca
    20941 aagacttctg taataggaaa tacgatagaa gtaaaagctg aacctatttt caatgatata
    21001 agaaaatcgg tgttgaataa atatgacagc ggaacagaaa agatcactgc tgctcaggca
    21061 tggcaaaatg caaaagtttt agcaaaacca gctattcctt cacagttttc tttctcatcg
    21121 ttagtagata cgcttgctaa tgtgaagata gaaaaagcga attttttaga attctttggt
    21181 ggaaaagagg gatctattct agatcgattt catggggaat ttctaaaaga taataacaca
    21241 ttacgtcata aaacaaggct aggcacggat cataaaatca aagcgattta tactaaaaac
    21301 ttaactggtc ttgacttaga gatagatgct caaagcgttt tagttggagt ttatccattc
    21361 attagcagtt cgtcagaagg agaggatgaa atcactctac cagaagaagt cattttcacg
    21421 gattacgtgg aagattatcc tgctggatat gtatcttttg ttgatttcaa agacaaagcg
    21481 actgatgtag ccacattaag ggaagctgct aaagactggt tgaaaacaaa tatagataaa
    21541 caaaaaccac aagtgagtgg ttcgattgaa ttagtaccat taaggcatca gcgaggctat
    21601 gaaaaatttg ttgatttaga aaaagtttcg atgggcgacg gagtagatgt gtatcatcca
    21661 cagttaaaag tgaatatgtc agcgagaatt gtggaatata cgtttaatgt tctaaccaat
    21721 tcatacgata aattagttgt aggaaacgtc aaaacaaact tcttagaaaa cacagagaat
    21781 aatgtcagca atttgattaa tgatgccatt gatcaattga aaaatggtgg cgaaatcagt
    21841 gatttaatca atgatattgt agatcatcaa actgatatga ttactggcca agatggtggg
    21901 tatgttttat tagatcctaa agaagcacct agtcgtattt tgattatgga cacgccagat
    21961 aagaatactg cacggaacgt tttgcaaatc aacaatgcgg gtattggttt ctctaaaact
    22021 ggtattaatg gaacgtatga caccgcatgg acgttagatg gcggattcaa tgcctcgttt
    22081 attacagctg gtgagatagt agggattact attagaggta ctacattaat tagtgatggt
    22141 gctgattata gaacaagtat tgctaatggc aaaatgactt ggtactcaaa aaaagttaac
    22201 aaagatatta tggagctaga agcacgtgat tatgtaagtg ctgatgccgg tattgtatca
    22261 tacaccatga aaactggtgg tggtttcatg attagaaatc cacagggtaa cttggttttt
    22321 agtacgtggg ataatggtaa taacagaccg tttttatctt ttggtgcgcc aaatttcaga
    22381 tacagtaatg ctagttatat aaatgatggt gacggtaatt ctttaggtat tgatggtagt
    22441 gcaggtaatt catgggaatt taaagtagct ggtaggacta tgaaatttac tagtgacggt
    22501 atgctaacgt taccaggttg tttttttggt tcatgggaag atgggaaact tgctaggttt
    22561 gaacaatcaa cggtacaagt atataaagat tttactgtta gaggtactaa aaactcaacc
    22621 gtaccaacag aacattatgg acaacgacta ttaaacgctt atgaaactcc agaatattat
    22681 ttcgctgatt atggggaagc cgttacaggt gataatggta aagttcgtgt tgatattgac
    22741 cccatgtttg ctgagacagt aaatctaagt cggtatatga cacatgtgac acctacagag
    22801 ctagttttgt gcgcagttac tcacgaagat attgaccatt ttatcattga aactagtaag
    22861 ccaaacgtat tagttagatg gaatttagtg gcacaccgtc tagggtatga agatattaga
    22921 ttaaaagagg atacagcata tgatagcaca gtgcttgacc aaaaacgttt ttaaaacgaa
    22981 gacaaggagg tatataaatg gctagcagtt tatataattt ggctttagat ttcagcaaag
    23041 aattaaacta caccaaagct attatggctc gtcaaggtga taaagggatt acggtgactg
    23101 ttaaaccatt tttaaatggc ttgcagatgg atacgagtgg cggaacattt actttaaaag
    23161 gaacaacgcc atctaaccgt tacgtagata gtgtggcaac tagtgtaact agtgaagaag
    23221 tcacgttttc tcttgatggc acatttatga gtgaagcagg atattataaa cactgctacg
    23281 tagaatatag aaaagacgat caaattttaa cgacgcaaga tatcattttt ttctcactag
    23341 gagtgtctga catttcgcaa ggccaagccg atgaatacgt ttcgcaattg gaagagttga
    23401 ttcaaaagta taacgaaact tttgatgctt ttatggctga aatcgaaggt agagtggaca
    23461 gcttaaatca acagattact gatttaactg gtcaagccaa aacactacaa gacaagttag
    23521 atgctctgaa agaagaaatt tctaaattag gtaacttgca tgtgatgtac tctaattcat
    23581 tagacttcgg ggactatgat tatagtggga atccgaattt aatggctaat ataaacgctg
    23641 acagcttctc tcaaggtact ggtgctttat ctgttattga tgatggtgac gaggtagtga
    23701 tcacgcttga cccaaaccat aaattagata tatacaagat gaaaagtcaa ccagctctgg
    23761 tagtaggcga aacatataac atgagcatag aaattatgtt agaagatgat ttcactggag
    23821 atcctagtaa gatagcacta agatatttta aaatgcctaa ttgggttcca gcactaacta
    23881 cgcctaatac attaactacc gctaagggtg tatggcaaaa gctaactgtt accgctaaaa
    23941 tgactactgc gattgataac gctgaaagtt ggtatgtaac actatataat caagacgcta
    24001 ataacagcct atctggtaga ctacgtttga gacacgctaa acttgaagct ggttcaacag
    24061 caacaccata tcagccaaat ttactagttg aaccttggag cataggcaaa gtaccattgg
    24121 gtgataatat taaagacaaa ggtacagtgt ttcctattaa aacgagtgca tatgcagtgt
    24181 atagcggtgc aaatacggaa ccttatatag caggtcaaaa atatacatta actatgaaag
    24241 ctactaaacc agcaacgcaa acatttaatg tttttctgga tgccggaaca ataggtgtag
    24301 gctcaatgac ccctgtagat ggtttaactg atgtttggca aaagacattt actgttagtc
    24361 aagctaatat agatgccggt gtaacaaata gacttgcaat ttatcaagta cctaattcgt
    24421 ccgtaggctc tgttcaaatt gactggctca agatcgaaaa aggcgacaca cgaaccccga
    24481 atattagtga gtataaatat cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt
    24541 atgtttggga tctagcacca gaatatgtcg aagataattt ggccacagat attaaaattt
    24601 ctgaaattac tggtaaagca aacaattata ccgatgggaa agtatcggag attaattcgc
    24661 agttgactgc ttcaattaat gaagtagaca ccacagctaa ggatgctcaa acaaaagcga
    24721 atgctaatgc gactgctata gatgaattag acaataagat cgatgaacgc attaatgata
    24781 cagctactac cacattaaca gttacaaacg ggaataccgg atcagcaaag ctttatcgtg
    24841 aaggaaaaac agtttctata tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg
    24901 attcaacgat actaacaatt ccagaaggct atcggccacc aattagtttt gagcaactgg
    24961 ttggctcgat agaccgttct actttgaaca gtgctcagtt atctattggt gcagatggag
    25021 ccattaaatg gcgaagaaac tcaagttatg gatcggatta tacctttgca attacttaca
    25081 cgatttagaa aagcgtgaat cgatatgaag gcagcatata gaccaattga accttacgga
    25141 ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg aagaatgcac agaagtcgaa
    25201 ccaccgattc caaattggaa accgaaattc aattactgtg agggaaataa atgaaaaaca
    25261 tttggaaata tggacgtact ggcggagagt acgcaggaaa agtattggac gacatgcttg
    25321 tatccgttcc ttacacagat cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt
    25381 taacaatcgc tgatcaaatg tttgatccta aattgaatca atggattatt ttagcgaacg
    25441 cactagatca caacgattta aacaatctca aagcaatgta tgagtcgtta gaaaatgaga
    25501 acggcgattt aaaacagatc aatgccaaac tcatgctaag cgatgtagca attaaacagg
    25561 aaaatactgc attgaaagaa aaagcggata gtttagcaca aatcaattca aaaatgatgc
    25621 ttgcttcgtt acaaaatagc aaagacattt cagaaattaa agagcaacta aatccagctt
    25681 caaagggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta tgattggggc
    25741 tgtttcacta acgaacaagt aatggttttc gttccgttgt gcattactga agaa
//
LOCUS       DAZKCC010000037        24634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000037 DAZKCC010000000
VERSION     DAZKCC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.037"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..287)
                     /locus_tag="ACG8VH_001552"
     CDS             complement(<1..287)
                     /locus_tag="ACG8VH_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9110109.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIR"
     gene            complement(314..904)
                     /locus_tag="ACG8VH_001553"
     CDS             complement(314..904)
                     /locus_tag="ACG8VH_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFP9110110.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(1222..2283)
                     /locus_tag="ACG8VH_001554"
     CDS             complement(1222..2283)
                     /locus_tag="ACG8VH_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9110111.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(2851..3918)
                     /locus_tag="ACG8VH_001555"
     CDS             complement(2851..3918)
                     /locus_tag="ACG8VH_001555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9110112.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(3938..4417)
                     /gene="pssE"
                     /locus_tag="ACG8VH_001556"
     CDS             complement(3938..4417)
                     /gene="pssE"
                     /locus_tag="ACG8VH_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFP9110113.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(4434..4895)
                     /gene="pssD"
                     /locus_tag="ACG8VH_001557"
     CDS             complement(4434..4895)
                     /gene="pssD"
                     /locus_tag="ACG8VH_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFP9110114.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(4909..6159)
                     /locus_tag="ACG8VH_001558"
     CDS             complement(4909..6159)
                     /locus_tag="ACG8VH_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFP9110115.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(6537..7988)
                     /locus_tag="ACG8VH_001559"
     CDS             complement(6537..7988)
                     /locus_tag="ACG8VH_001559"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9110116.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(8309..9073)
                     /locus_tag="ACG8VH_001560"
     CDS             complement(8309..9073)
                     /locus_tag="ACG8VH_001560"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP9110117.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(9101..9799)
                     /locus_tag="ACG8VH_001561"
     CDS             complement(9101..9799)
                     /locus_tag="ACG8VH_001561"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP9110118.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(9811..10590)
                     /locus_tag="ACG8VH_001562"
     CDS             complement(9811..10590)
                     /locus_tag="ACG8VH_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP9110119.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(10606..11550)
                     /locus_tag="ACG8VH_001563"
     CDS             complement(10606..11550)
                     /locus_tag="ACG8VH_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9110120.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(11596..12375)
                     /locus_tag="ACG8VH_001564"
     CDS             complement(11596..12375)
                     /locus_tag="ACG8VH_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110121.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(12756..14831)
                     /gene="glyS"
                     /locus_tag="ACG8VH_001565"
     CDS             complement(12756..14831)
                     /gene="glyS"
                     /locus_tag="ACG8VH_001565"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9110122.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(14833..15750)
                     /gene="glyQ"
                     /locus_tag="ACG8VH_001566"
     CDS             complement(14833..15750)
                     /gene="glyQ"
                     /locus_tag="ACG8VH_001566"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9110123.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(16136..16954)
                     /gene="recO"
                     /locus_tag="ACG8VH_001567"
     CDS             complement(16136..16954)
                     /gene="recO"
                     /locus_tag="ACG8VH_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9110124.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(17097..17996)
                     /gene="era"
                     /locus_tag="ACG8VH_001568"
     CDS             complement(17097..17996)
                     /gene="era"
                     /locus_tag="ACG8VH_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9110125.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(18011..18406)
                     /locus_tag="ACG8VH_001569"
     CDS             complement(18011..18406)
                     /locus_tag="ACG8VH_001569"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9110126.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(18390..18866)
                     /gene="ybeY"
                     /locus_tag="ACG8VH_001570"
     CDS             complement(18390..18866)
                     /gene="ybeY"
                     /locus_tag="ACG8VH_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9110127.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(18895..21087)
                     /locus_tag="ACG8VH_001571"
     CDS             complement(18895..21087)
                     /locus_tag="ACG8VH_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP9110128.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(21111..22082)
                     /locus_tag="ACG8VH_001572"
     CDS             complement(21111..22082)
                     /locus_tag="ACG8VH_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9110129.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            22787..24166
                     /gene="brnQ"
                     /locus_tag="ACG8VH_001573"
     CDS             22787..24166
                     /gene="brnQ"
                     /locus_tag="ACG8VH_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9110130.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            24448..>24634
                     /locus_tag="ACG8VH_001574"
     CDS             24448..>24634
                     /locus_tag="ACG8VH_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9110131.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         7053 a         5131 c         3988 g         8462 t
ORIGIN      
        1 cgtatccccc agataatcaa tcctatattt tctaaattaa taactgagct acaaatagaa
       61 actagtaaaa acaaaactat cagcatctca tttttgctag catatattcc ttttctgcgt
      121 ctaaagctaa gtgcatttaa taacacaaag atattaatta tatctcccaa atataaaatt
      181 gctcttgggg cgttaaaagt atatacaaga actatggaga ttgcaaaaaa tgccaacgtg
      241 aacaatgtcc aatctgctcc tgaccatctt tttattgaaa tacgcatcgt tattcacctt
      301 ctttcgctaa aaattattct aaaaatactt ttgttaaatt aacataccct attatttttg
      361 ccggtacacc tgcaagaatt gcaccattct ctagacaatc tttgataaca atagctcctg
      421 ccgcaacaac tacattgtta gctattttta cattaccaat aatctttgca cccacgcata
      481 cacgtacatt atttccaatt acaggacact taggatcgat tccatcattt ccaatacagt
      541 tattcccata taagtaacaa ttttttccta ttatggcgtt accattaata acaattcctt
      601 gtgtatggta aaatcttaca ccttcatcaa atacattttc accacactct ataccacatt
      661 ttcttcccaa actattcatt cttctgcaag acacaatata cataatcgca taaatgatat
      721 ttttctttct ctttgaataa taataagatg taattctcat tcgacgagta taccgccaag
      781 cgttgtagtc cggatgtgat ttcattattt ttagtaattt tgtgtgttgt cctttagatt
      841 ctatatatag attcttttcc atacgaagaa ccgcttttaa atattcttta ctgctaattt
      901 ccattcatat ctcctattaa ttcaatttat acttaacaat tttattcgga tttcttgcaa
      961 caagagcatc attcaatgct actgaatcta caacgatgga tatgttatct tcatcgttga
     1021 ttccaccgga tacaatatta cttataaaaa tcgtaacatt ttttccaaaa gaagggatgt
     1081 ccgtgatgta taatgcccat tttccgatat ttccagttat ttaaacctaa cgaattttta
     1141 caatagtaca agtatcacaa tcattaatat ataactggaa tataaatctc caaaaattca
     1201 ctatagtaca gttttttatg attaaaatct cctatttcta cccattccgc ttaaatatgc
     1261 taatgtttta accccaataa tggattggtt tagtaaaaaa tagttaaata ttcgttgttt
     1321 aattggattt tgtttatatg ggtatgagtg aatacaggca atcaactcat cgttcataag
     1381 tatcttagac aaataggagt ttaaatcaaa ttctttatta aaactagcat atctaactgc
     1441 cttttgaata caagtacgac agcgtcccaa caaaagtctc cttgttttgt ttacaaatat
     1501 ctcattttct aatatatagc ttaaatcgcc ttcaattttt ttaactaatt caatattaca
     1561 atcgaactca ttatctctaa aattatgtgt caatgaatta ttattattac atctataata
     1621 atagatacaa tctttaataa aacttgttat ttttgaatgt tgcaaatatg aaatatggaa
     1681 tatgatatct tcagatatat aatccctttc tgacggaaac ttgatattgt gctgttgaat
     1741 tatctcagat gaatacagcg catgccaaac tgacattggc aatataaaat cgactttata
     1801 actcggctca gtgccaatca ttccaagcaa cactttttca attatagcgt tatttgtaaa
     1861 tatcgtacta tcatatcttg ctggctttga aataatatta ttatcatcat aatattcata
     1921 atatccagca taacatgtgt ctgcattact cctttttata ccatttaaca ttgcttcaat
     1981 ataattagct gcaacaaaat catcagaatc aataaaagca acatattttc cttttacatg
     2041 ttcaagtcct gtatttcttg ccaatcccaa gccagcatta tttttatgaa taatacgaat
     2101 ccttgaatcc tttaatgcat agtcttcaca tattttatta gatgaatcct tagatccatc
     2161 atccacaatt ataatatcta ggttcttata tgtttgatta cacactgatt ccaaacaact
     2221 tcctacatac ttttcaacat tatatacagg tatgattaca ctaattagat cactcgaatt
     2281 catccttcta tcctttcact cacgttttct caattagtca atgacggcat caacgatttt
     2341 cttaaattct ctgaaaaaaa tagactctga tcttgatgat gtgaatgaca tactatcttc
     2401 ttctagaaaa attatttcct ttaaattatt tattaaatca ataatatgat cctcgtaagt
     2461 atgctttccc ttggaataat gaaaatatac ttttgtatta cacaatattg caattttatt
     2521 tcttaatatg attatttcac tcattcatta ctgccacata tacacaactt ataaccaccc
     2581 cctgcaaagt ttagggtaag ttatcagata atatcttcct cttcatcatc cagaatacaa
     2641 aatctattta tttatagaaa tatcattaat gtttttatat aatgtaccgt gcttgtactc
     2701 ttgctctatt acaagcacta aaacactact attcatttga attcttacac ggataaaaat
     2761 ttacatcgtc tatttcaata ttttttctct ctattttatt cacttaataa gttcattaca
     2821 taatcgaaac attttttatt ttccttgtca tcactccttg ctcccttttt gatacagatt
     2881 taaaattcta tcaaagaaat ttccatcgtt aaatttcgtt attgccaaat ccttcgaaca
     2941 acgtgaaaat tttacgtatt tatctttatc attgattttt agtattgcag atgtcaacgc
     3001 tgatacgtca ccaggttgaa acaaatatcc attttctcca tcacgtactg ctgaagaaat
     3061 atctcctaca tctgtagcaa ttacaggcat tccataagaa atcgcctcta gaatagatat
     3121 aggcaatccc tcgttatagg acggtaaaat catcacattt gatcttctat agcactcctc
     3181 ctttttttct cccacaaccc aacctgtaaa ttcaaaatac gattgcaaat tatattcttc
     3241 tatcaattct ttgagcgata tttcttcatc acctgctcca gcaatctcaa aaataaactt
     3301 tttttctgga tattgttctg caatgttttt aactgcttct accaaatcgt gtacaccttt
     3361 tcgctttatc aaaactccca taaacagaaa tcggcacatt tttgaattcc actttatggt
     3421 atattctgga actacaatag aattttctac aacaacagtt ttacaatttg gctcaatttc
     3481 tttaattatc ttcttccatt tttctccaag tacaatcgtg gaatcagact ctcttaacag
     3541 caagcgaatc tgattttgtt tttttacaga aacactatca taccattttt taaattccga
     3601 tccatgaaga tggatgatta caggaatatt taataatcta aaccacttat tcagcacata
     3661 tttcctacta aaactccctc tgtacgacat gtgcatatga ataatattag gtttactcaa
     3721 taaagagata cctataattt tagcaaatgc atacataaaa tataaagatt taaatattgc
     3781 atttccaccc ttataactcg gtataaaagt catctcgata ttcttttttt cccaattaaa
     3841 attgagcatt tgtgtaatta tacttgtaat tccacctttt accgagctgt cattaccaat
     3901 cataagtact ctttgcataa cgctttactc cttatattta tattttttct attaatacct
     3961 ctatcgaatt aataatcgtt tccgtgttag atacatactt gttaaacatc cgaactcttg
     4021 tttctttaac ggcatcttct aaattttcta attcataaca aggatagata atattcatct
     4081 catcaaattg tctaattaac tgcagttggt gatcatcgac atgctcacca tacttagcca
     4141 atcgaggaac agcaatgacc cttttacctt tttttacggc cccaattata gcacctgtac
     4201 ctccatgagt aatcaccatt tctgcttttt gcatagtttc agtaaactca tccctattta
     4261 gaaatgcttt ataggaataa tttagtggtt tataatcact ataaccactc tgtgcaaatg
     4321 tactttcgat tattactcct ttttcaatta aatcatcaat agcttttaac aatctatcaa
     4381 attgaaattt ttgtgaacct aatgttataa atatcatttt tttctcctat tatttaatat
     4441 atcccaccca aataaatagc attaggataa aattgtagca tagtttccca ctgaacataa
     4501 aattgatctg caaaatgata catcaatttt cctgtctgag tagggctggt tacttttgca
     4561 aacgattcaa tataaatcag tttttttccc atcatttttg ttattaagca gatagggatc
     4621 atagctaaaa cacctgtaca aatcacgaga tccggttttt cctttatata tataataatt
     4681 gatttgaata aattaataag tatagtacac aaaaaaaact tttcttttct atttacttgt
     4741 ctcatgtagt acgttttttc gccttttgac tcagctgaat attctgtctg ttctgttata
     4801 acaaaactct catacttctc catcagtggt tttaacatca ttaactgttc ataatgcccc
     4861 ccagaggaag ccgcaaaaca tatctttttg ttcatattaa cctctctatc agtcacgctg
     4921 aaaaatatct ctagtgtaaa ctttttcttt aatatcatcc aagcaggtat catatcgatt
     4981 ggcaataata gaatcacaca tcgccttaaa ctgctctaaa tcgttgataa ccttacttcc
     5041 gaaaaaagtc tctccatctt tcagtgcagg ctcataaatc accacagtcg cacctttagc
     5101 ttttacgcgc ttcataactc cctggattga actctgacga aaattatcac tgttgctctt
     5161 catggtcagc cggtatatac cgattaaaac tggattttcc ctgttgacat catactgatt
     5221 atattcagcg tagttgtaat agcccgccat ctgaagaaca cgatctgcga tgaaatcctt
     5281 acgggtacga ttagattcaa cgatagcctc aatcaaattt ccaggaacat ctgcatagtt
     5341 tgccaacaac tgcttggtat cttttggcaa gcaataacct ccatatccaa aggatggatt
     5401 gttgtagtga gttccaatac gaggatcgag acaaacacca ttgattatct gctgagtatt
     5461 cagccccttc atttcagcat aagtgtctaa ttcattgaaa tagctgactc gcagtgctaa
     5521 ataggtattt gcaaacagct ttactgcttc ggcttcagtg aatcccataa aaagagtatc
     5581 cacgttctcc ttaattgcac ttttctgcag aagtgttgca aaaacatgag cagcatttaa
     5641 caagcgctca tcagtcatat cagtaccaac aataatacga gatggataca gattatcata
     5701 taaagccttg ctctcgcgca gaaactctgg actaaaaata atgttcttac tccccgttgt
     5761 ctcacggact gattcagtgt agccaacagg aattgtagac ttgatgacca tgatggtatc
     5821 aggattcacg tccatcacaa cctcaataac tgactcaaca gcagaagtat cgaaatgctg
     5881 agtacgactg tcatagttag tcggtgtagc aataacaacg aaatctgcgg atttataagc
     5941 gctccttgca tccaaagtag cagttaagtc aagttctttt tctgtaagat acttttcaat
     6001 atattcatcc tgaattggag acttccgttt attaatcagt tcaatctttt cagggataat
     6061 gtctaccgcg gttacctggt gatgctgcgc caatagtgtg gcgatactca aaccaacata
     6121 accggtgccg gctactgcga tgttgtactt tttcttcaca atatctcctc tttcctctct
     6181 taagtatgga gaatgttact cctacggcta ttcatcatgt caaaataaca cagcttagaa
     6241 tgttgataaa ataattttcc agaatgtgat aaaatctctt tcatttataa accacacatc
     6301 taattttatc tattctccac aagacatgta gagtattctt taataacaag atacgacctt
     6361 actaaatact tcaaaaaatt atcatattta attcataaat ataaatcttc attttaaatg
     6421 tatgcccaaa aatttatctg taatgcgtca tcaaaatcta gattccaaat ctttatcaat
     6481 ttttatccac tcattctacc actttagtaa tattttctat ttataacact ttcgtcctac
     6541 attgatcccc ccctctgaag tacagttttt actgtcttaa ataaaatttt aaaatctaac
     6601 cccatactcc aattttttat atattccgtg tcaagttttg ctacttcttc aaaatcagta
     6661 atatcacttc taccacttac ctgccacatt ccagtaattc ctggcttaat agcaattctt
     6721 gcacgatgat gataatcata ttcttcatac tctgatttaa ggcatggtcg aaatcctata
     6781 aggctcatct ttcctgttag aacgttgaaa aattgaggga attcatctaa agacgtggat
     6841 ctaataaatt gaccgattcc tgtctttttt gttccatctg gaagtatttt atttccgata
     6901 attcttggat caaactccat cttaaacata agattattgc tcatcttatt ttctttcatt
     6961 aattctactt ttctttcttc tgcatccata tacatacttc taaacttata catcttgaac
     7021 ggtttaccat tctgacctat acgttcttgt gcaaagaaaa ttgggcctgg tgaattaata
     7081 taaataagtg gagcaataaa gataaaaata attcccgttg caatacatcc aacaatacct
     7141 gcaagaatat caattgttcg ttttataaat gcctgttgag ttgtcataac attgatactt
     7201 gttgtaagta ctgtatattc tcctatacga ccaatagatt gttttcccaa agcactatct
     7261 gatacttttg caagattcaa atgtacagta aggcccattt cagagcatcg ttcaataaat
     7321 tctttcatac acgaagtact tttattcaaa ataataaaga cttcatctac ccatccctga
     7381 cataagtaat ccgctgcatt tttggaatca gaaacaactg tcactccctc aacatcttca
     7441 ccaatcatgc ttttatctat gataatgaca ccatttattt tatacatttt atagttatgc
     7501 tctttcacat tacgaataac tacttcagca atactactag atgtaactat aataagtgat
     7561 ttgttcccct catttttcat tttatgtttc aagtaatgtt tccataagag gcgagttata
     7621 tatgtaatta caacgtataa acctcccatc aagaaaagaa cgactctaga ataatcattt
     7681 ccaccctgaa cactaatgag atacaaactt cctaacagca ccaacataat cgcttgcttt
     7741 acagaagaag caaattctat atagtaccct ctttttaata cacctctata tgcttcaaac
     7801 atgaaaataa ctaaaagatc tgccagttca atgaagactg ccatgttttg atacattgga
     7861 atcacataag gattccaatc tacctggcgt aagacatacg atagataaaa agcaagctgg
     7921 atacacaata aatctaaaat aataaaatca atatgtttaa cccatcccct tgaacttttc
     7981 ttgtacattc ctttttactc ccctcttttt aataaaataa attgtattac ttgttttctc
     8041 tcctaaatat ctttctgaag gattattgac tcgacaaatt ggcttgtgga tattcttata
     8101 tatagaacat ccgtaagcca acttaaaact ccttaacaaa atttttttac tcatattgta
     8161 gatcaaaaca agtatccaag aaatcatatt ttatttcttt tttctgctta ctcaacaaca
     8221 gctaaaagaa aaatcatcat aaacaaattc aactaagcca acgatcccct tgtgaaatca
     8281 agttttttct tgatttacga ataaagagct aaaataaacc aaacttcttc ttttttacct
     8341 cttcatagac tggataactg atttcatccc cgttgatcag atcctttgcg acttgtttca
     8401 tctgtactac tttttcctta ccaaagtcct ttgcaatctg ctcatagcac tcttttaagt
     8461 aaaaggtgcg cttagagacc ccatgggcat cagaagcagc catctgcacg agattatgtt
     8521 tgaccatcgt tttggctgtt ttctggatgt ctttgccaaa taccccaaca taacttggcg
     8581 cagtgagttg ggccaaacat cccatatcta ggaacggaag caaatgattt gggtcttcgc
     8641 gaaaatgcac gttgcgttct ggatggacga ttactggtgt ctttcctagt tctaacaact
     8701 caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg atcagaagat
     8761 atgtatcatc taaatctgta aataatattt catctcggtg gatgtcttcg atgagcgttc
     8821 cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt ttctctaatt
     8881 cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat
     8941 tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca
     9001 ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg atatggcaat
     9061 gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg tagtaataac
     9121 cagcatcttt gctatgttct gctccgttgt acacaacacc taagactcgt gcttgtacca
     9181 tctccagcag ttcttttgct ttagtcaagg attctttacg tgacatattt tcacgtacga
     9241 ccaataaagt gccatccgtt tttaaagaca tgatctgtgc atctgttacg gctactactg
     9301 gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcggatagg atctgatcca
     9361 ttcttggcga gcttaacaat tctgatgggt tgggtggctt aggtccacta ggcaaaatcg
     9421 acaagttctc gactggcgtt cgttggatca catctgctac acttcctgat gaactaagtg
     9481 ctgtacttaa tccactggca ttgttcagct ggaatgtctt atagacgacg ggtttacgta
     9541 aatctgcatc gactagtaga actcgttgac ctgatttagc aaaaacgact gcaatgtttg
     9601 ctgcagtggt ggatttccct tctccaggac ctgatgaagt cacgacaatc gttttgattt
     9661 gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga
     9721 ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt gctgcttttt
     9781 gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg
     9841 ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt tacggatacc
     9901 agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga atcctaatgt
     9961 atctgtcaca tatttttcat ctttcactgt ccgatcgagt aattccaata agaatgccag
    10021 acctacacct accatcattc ctaacattaa gccagtcatt aaattcaatt tattatttgg
    10081 cgaaacaggt gtcgtattgg caactgcacc tgaaatgatc gagattttat caacattcat
    10141 cacatctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct gttgagcagc
    10201 cactgcattc gtacttattg cctgaatcga gaacatttgt gaattttctg cctgattaac
    10261 tgagatggca tctttgattt gttcagcagt cattttcaca ttgtcttctg tttctaaacg
    10321 atctttgact tcattgagca ctaaatcact gacgatcata tctttatacg tattgatcat
    10381 ttgcaggttc gtattgacat cattgacatt tgcagtgtct gattgtggca gtgtcacgat
    10441 caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat
    10501 ggccaagcca gcaaacattg agatgatgat tattaccaag tgtttgcgta atgtttcaaa
    10561 aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt tattctctag
    10621 attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcac tgactttttg
    10681 ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct taatcgtacc
    10741 aaatgcctcc cgataatcta aagcaatttt tttcatttca tcaaagttca tgtctgtttt
    10801 catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttagactcaa
    10861 tacttttttc gctacgcctt ctacgatctt tctttggcgc ccttgtctac cgtaatcacc
    10921 atttgggtct tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtaat
    10981 tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat
    11041 accaccaact gcatcaacga gttcctttag tcctttcata ttgatagaga catagtgatc
    11101 gattgggatg tctacatatt tttgtacggt atccatcgcc atggcagctc cgccgaatgc
    11161 gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatctcg
    11221 aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt
    11281 atctgatctt ccttgatcga cccgacctaa atctccagta tcaatcccca tcaatagaac
    11341 agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc
    11401 cactgattcg taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac
    11461 tcctacaatt gctaatacta atacgataag aactgctaaa ataatcaaaa atattttttt
    11521 ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa
    11581 tcaacgtttt aactgtcaat agcttatatt gctatagccc atttgctgag caacagtttg
    11641 tgcttttgtt gcgtatgcgg ctcttatttt ttcattccgg atctgctcta tttgactgag
    11701 cgccgtcaaa tattgatcgt aagtagcggt agctgttact tgaccgtctt tcagcatcgt
    11761 atcgaatagc tgatctagtt gcgtgacttg tgcaatttgg gtatcagcga atcctagata
    11821 ttgcataacg actgttttcc aagcactatc cttaaaaaag ctcatatttt cacgtacatg
    11881 ggcgacatct gcacttgcca acttttcttt gatgatgaca tcatccacag cttgctgcca
    11941 gtttgatacg tttttagtaa ataatttatt gacagccgtc tggatcttca gttttgattc
    12001 tgcatcctct aactgcgtta agactgcttt cttttcttct tggatcgcag cagcttcttt
    12061 aggtaattct gattccttga tgtcataatc atctgccgaa agtttgatag aagatagttt
    12121 atgtgacaaa tttgaaactg tttcttctgt tatttcagga tttaggaaaa ttttttcttt
    12181 tgtactgtac agcttatcta cttcttttga taaagaggtc acggtgtctt tttgattggt
    12241 gatggcagat tctgcgtaat tgatccgttg atgatattgc cgctgattat tgagatggta
    12301 tccaaacaag caaatcacaa tagcgcctgt gcctaccgcc acagtagcca ataacttgac
    12361 gaacggcttt tccatagatt cactcctaaa tattgatgat agataagtaa agatgtgatt
    12421 cattcacatc agaatgagcg ctttttattt taaaagcata attaaactaa tatattcata
    12481 gaaatattta taaacaggtt ttccctatat aatcatacca catacagttt ttttgactag
    12541 ggaaattcct aatttaaaag taaataaaac agtaacaaga ttataattaa tcgttttcat
    12601 ttttatttcc tcacttttca tgagcgtttt gaaaattcga acaaagaata aaataacaaa
    12661 aaattattaa aatagaatta ataacagcta tttataacaa aaaaaacaga gacacagcac
    12721 gtttcgtgca tgctctctgt ttttcaaaca aaagactatt ttgtgatgat ttgatccaag
    12781 cttgctaatg acaaagccat ggcagctatt tgacttagtt gtgttagtcg gttcttacgt
    12841 actgctggtt catcagccat gaccatcgtt tgatcaaaat aattttcgat cagtggacga
    12901 agagcgacaa gctggtcgta gttttcgtgg atcgtttggt cagcaaaact tgctttcatt
    12961 gcttctactg ctttgtgtaa ttctttctct gcgtcgtttt cgaacagctc agggtctact
    13021 tctgattgtt tttcaagctg cgcttttttc gctaagttca cgacacgtgt caaagcttcc
    13081 attgaaggtt tgaagtcttc atctgcaaga tgttgtttga acaattgtgc tgctgcaaat
    13141 acttttgtca gatctttttg ttccgcattc aaaacagcat cgatgacatc atgacgaata
    13201 ttcttcgttg tcaataattg gcgcagacgg cctttgataa agtcgatcac ttgttgttgc
    13261 ccttctgata aaccaatgcc gaaacgatcc gtatctttac tgataacatc gctaatcgtt
    13321 tcttgcagaa cagacaaagg aaaggcccat tttttcgatt cgatgatccg aacgacaccg
    13381 tatgcttggc gacgtaaggc ataaggatcg tttgacccgc ttggaatcat cccaactgca
    13441 aagaaagaaa agatgctatc taatttatct gcaatcgcta aaacagaacc tactgtagac
    13501 tgaggtagct ctccttcact ggatataggc atgtaatgct cacgaatcgc tgtcgctaca
    13561 gcaggtttct cgcctttcag aagagcatat ttttcgccca tgatcccttg tagttcagga
    13621 aattccccta ccatgttggt tactagatca aatttataga tttcagcagc tcgttgcaaa
    13681 tccatcaatt cttcctcaga tagtcctact tgttcgccaa tgatacgggc aatgacttgt
    13741 acccgttgca ttttctcata gatactaccg attttttcat ggaaagtaac agtttttagt
    13801 ttttcaacgt attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct
    13861 tctaaacgag ccgtcaatac tttttgattg cctcgaacca catcttccaa ttttacccga
    13921 tcaccattac gagcagaaat aaaatgagga agcagcatac cttgatcatt ccgtacctcg
    13981 aaataacgtt gatgttcttt catagaagtg actagtactt cttcaggaac agaaagatat
    14041 ttttcttcga agtcgccaga aaagacagtt ggatattcga ctaaattgtt gacttcttct
    14101 agtaaatctt cgtctagatc aataacccag tgattttttt ctgctaattc gtttgcttgt
    14161 gtaacgatca tttcttgtct tttttgcgga gacgcaatga cgaattgttc attcaatttt
    14221 tcttcatatt cttttgcatg cgcaaatgtc acatcttcgc ctaagaaacg atgacctcga
    14281 gaggctctgc ctgttttgac atccaagatt tcgaatggga ttacttcgtc atctaacaat
    14341 gccacgatcc agtgaatagg gcggatatat tcaaaatcgt attctgccca atgcatcgtt
    14401 acagggaaag tcaaactttt gatcacccct gctaagcctg tcaatacttc ttttgcttcc
    14461 ttaccatgag tgtatttcgt tacatagaca tattccacac cttttaattc tctaaaagtg
    14521 atagcgtctg tcgtcagacc ttgtccgcgg acaaatcctt ccgctgcttt cgtccagttt
    14581 ccatctgcgt ccaaagcaat ttttttggct ggtcctttga cttcttcttc actgtccgct
    14641 tgtcgttctg gaatatttgt tactttgatt gctaaacggc gcggtgtgga aaatgtttcg
    14701 atcgtatcat aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt
    14761 ttcatgcttg gtgtgacaac atgggcaggc atttcttcca gcccgatttc aagtaataag
    14821 tcttttgcca tcttattcag cctccttcac ggtttctgga tttgctgtgt ttttattcaa
    14881 caaagggaat cctaattttt cgcgttctgc cacaaagatt ttggctacag cacgagccat
    14941 gttgcggatg cgagaaagat aaccggcacg ctctgttacg gatactgcac cgcgggcgtc
    15001 taataagttg aatgtatggc tacatttcaa gatataatca tacgccggat gaacaagatt
    15061 ttcatcgata cagcgtttag cttctttttc aaaacgatca aagttgctta gtagcaagtc
    15121 ttggtcgctt acttcaaaag aatatttgga atgttcatat tctggttgtt tgaagatctc
    15181 gccatatttc acgccatcag cccattctaa atcatagaca ctttcaactt cttggatata
    15241 agaagcaaga cgttctaatc cataagtgat ctcagaagtt actggtttac aaggcagtcc
    15301 accaacttgc tggaagtacg tgaattgtgt gatttccatt ccgtcaagcc atacttccca
    15361 acccacaccg gcacagccca tggatgggtt ctcccagttg tcttcaacaa aacggatgtc
    15421 gtgttctaat ggatcgattc ctaaaaggcg caagctttct aaataaagct cttgaatatt
    15481 ctcaggtgaa ggtttcatca ccacttggaa ttgatgatgc tgatacaaac ggtttgggtt
    15541 ttcaccataa cgtccatcag ctggtcggcg agatggctct acgtaagcag cattccatgg
    15601 ttctggtccg attgcccgta aaaaggtata tgggctcatc gtacctgctc ctttttctgt
    15661 gtcataagct tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat
    15721 catatcttgg acggtcaatt ttttactcat ctgttttgcc cctttcaatt ttaaagcgtt
    15781 gctgtcaaat aagaatacag gaaagaaaac gacaaaaaat ccctatgtct gattacagac
    15841 atagggacga ataattcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt
    15901 tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc cggctcccac tctcccgaac
    15961 tcgctaaacc acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt
    16021 cttgtttcta taatagatcc tatcactgaa cgatctattt aatctatggt taataataga
    16081 cggaactccc tgtgtttgtc aaatggtttt gattttttgt tgtatgtttt atttactatt
    16141 cttctttttc aggtgtctct ttttcttttt cagacatgtc tttctctgac gtctttaata
    16201 cgtgtgccca agacttcatt tgattgatga actttttgct tttcaaatgg ataccgacat
    16261 attcatcata cagctggtcg atgagttcac ggattgcttg tttggtttct tctttcagat
    16321 ggattccact aattttatca taagaaatag ctgaaaacat ccgaacgaaa taaacagctc
    16381 gaggatctgc atgataacgg tgatcgtcca tcgaccaatg tctttcgcat atcacaccac
    16441 catatttgga agaatagtca aattttcctt ttgtttcccc gcatacgacg cagtgtgtcc
    16501 agattggtgc aattccgaag cgttgcagta tttggacttc aaaaatattc gtgatgattt
    16561 ctgcatcgtc tccttggtcc atccgttgca gtgcttgttg cgtaaactgg aacaaatgcg
    16621 ggtcgtactg atgatcttcg attgccgcat ctactagatt caataaatat gtgccatagc
    16681 cgctcaaaaa gatgtcttct tggatgtgtc gaaacgcatg gacatctttg acactgctta
    16741 aaaatgacaa accttcactt cgaaaattcc cgatataaac tgcttcagta aaagggagca
    16801 aagccggagc taagggctga ttttgccgat ggatctcttt gatataaaac atcattttgc
    16861 cagaagattc tgtaaagatt ttgactaatt tatctttttc tttgtaatta cggctagata
    16921 aaatcaagcc ttttgattct gcaagctggc tcatattctc cctcctaaaa aataaatctg
    16981 gaaaagcaga ttctgccgat ccagatcatt tatgttgcga tcttccgcag cttttctcaa
    17041 cacgcggatt tttattcagt ttatttctat acctaattta caagaagctc agaatatcag
    17101 tattcgtctt ttcggtagcc gaagtcttgc agatagactt ttttgtctcg ccagtctttt
    17161 tgaactttga cccaaagttc tagaaagact ttgtctccta aaagattttc gatatctttt
    17221 cttgctttcg ttcctacttg cttcagcatt tttccgcctt tgccgataat gatccctttt
    17281 tggctatctc tttcgacaat aatcgtagcc tggatatgga ttttatcatt ctcgtttcgc
    17341 ttcatcgaat caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag
    17401 actttctcac ggatcaattc agaaacgatg aagtattcag gatgatcagt cacttgatca
    17461 tctggaaaat attgtgggcc ctctggcatt tcactgacta atacatccat caaacgttcg
    17521 acgttgtttc cttctgtagc agagattgga atcacttgtg caaaaggcat ctgcgttgaa
    17581 taatcttcga tgattcccaa caactcgtct ggatggattt tgtcgatttt attgataatc
    17641 agataaacag gagaatgcac gttttttaga cgttcaataa taaaatcgtc tccccgaccg
    17701 cgtttttgat cagcactaat cataaataag accgcgtcca cttctttcaa cgcactgtaa
    17761 gctgcttcta ccataaaatc tcctaaacga tgctttggtt tatggattcc tggtgtatca
    17821 ataaaaatca actgagcttc tggtgtagtg tatactcctt ggattttatt tctagttgtc
    17881 tgtgccttgt cactcatgat ggcaattttt tgacccacga ttcgattcaa taatgtggat
    17941 ttccctacat ttgggcggcc gacgattgcg acaaaccctg atttatgttc tggcatatat
    18001 gttctccttt ttaattaaac aacaaatgat atattttagg gacaaataaa atcaacccaa
    18061 cgatgacggt aaaacaagcg gtcactaaaa ctgctccagc tgccatatct ttgatttttt
    18121 tcccaatcgg atgaaaatga aaatccgtaa acatgtccac gacattttca aaaacagtat
    18181 tgatgatctc tactatccat acgagaaaaa cagctaaaag cagccataac cattctatcc
    18241 gatcaagttg gaaaatcacg cccataaaca aagcgaggat tccgaacgca acatgtttcc
    18301 tcatgttgcg ttcttcctca aaaacagttt tgactccttg gacggcaaat tccacggaca
    18361 tcaggaaatg tttgtttttc tctacgtttt tatcttttaa gtccataggc atctaagatc
    18421 tctttctgta aaccaaacat ttctttctca tcctctggtg tcatatggtc atacccgttg
    18481 atatgcaaga atccatgaac cgctaaaaag ccaagttccc gatcatacga atggccgtaa
    18541 gcttctgctt gctctgcagc acgttcagta gagatcatga tgtctcccag tacacgaggc
    18601 aatggattct ctccatcttc aaaaatcact gggatttcgt cttcgccctc ttcttccaag
    18661 gcaaaactga tgacgtctgt cggtgcatct tttccacggt aatcacgatt gattacttgg
    18721 attgctgcat tgtccatgaa agtaacagac atttctgttt cttctggcaa ctctaaatat
    18781 gatgctgcaa attgcagcaa gtcgtcgatt tctttcattt tctcttctga gacttggttt
    18841 gtttcatcca tgaaggtaat atccattata catcgcttct ttctattctt cttgctattc
    18901 ttcttgttct tcttttatct tctcaacttc cgatttcatt ttaggatatt tgatccgaga
    18961 atggaaggta ctgcttaaac cgctgatcaa tgatttttct actttacgga tttcttttaa
    19021 cgtcaatcca ctgtcgtcca attgtccatc ggaaatacgc tcttcaataa gattatggac
    19081 aaaactttcg attttttcgc tcgttggatg gtccattgcc cgtactgccg cttcacaact
    19141 gtcagctata ttgacgattc ctgcttcacg gctttgaggt tttggtccag gataacggaa
    19201 ttgctcttcc gtaacttccg ggttgcgttc ctttgcctta acgtagaaat aacgcatcag
    19261 cgtggttcca tgatgctgtc tacaaatatc gatcaccatt tgcggcatat tataatcttc
    19321 caatattttg gcaccatctg tcacatgtcc aaagataata tgtttactgt cttctggcaa
    19381 taagaaattg tgcggatttt ccgctcctga aggcaagttc tcaacgaaaa agctggcgtg
    19441 tttgatcttg ccgatatcat gataatagca agctactcga gttagtaaag aacgtccacc
    19501 gatttctgct acagcattag cacttagatt ggctaccatc atgctatggt gatacgtacc
    19561 tggtgcttct tccagcaact gtttcaataa aggatgattg ggattgctga gttcatttaa
    19621 tacgatcaca ctatcatcat tgaccatcaa ttcaatatag gggtgcagac ctatcgtcag
    19681 caagaatgaa aacagtgcac ctgtgaatcc gcagactagc gttaaccaag tcgttgcatc
    19741 cccaaagctc attccttggt agataatcaa aatgacatcc atcatcacag gaaaaataat
    19801 gacccacata gcagcggata acccttgttc tgagatccgt ttgcgtctga cgatcgttgc
    19861 catgagacca gaaaatacat aggacatcaa aataactgtc agaaagttcg tgccaatcga
    19921 atcataaaaa atgaaaagtg cggatactgc ttgaaaaata ccagccatga tccctgctct
    19981 tcgattcaag aaaaagctta aaacaagtgg agcaaaagcc gcggggtaaa acagcggaat
    20041 ataagcagct tgttctgttt ggaagagttg gaaaaatttc attagtacga cgcttactga
    20101 cattgccgtc acgtagaaaa gtacgtattt tgttctttct cctgcatttt ggaattgaag
    20161 tgaattataa atcagtacac cgatttgaag caaaacagct aaaaccatgg caatgagtgg
    20221 gaaaatagaa gtcgtttgat tcgtcaatcc tagtaattcc aattttttca tcgctgaagc
    20281 atcaatttga tttccttcgc gaacaataat ttctccttgg aagatcatca ctggttgtac
    20341 agcttctcgt gcagattgtt ttaattcttc tgtctttttc tcattcaata catcattaac
    20401 tacgatgcct tgatccacta agtagcgaat catttgttgc tgctctgttg tgacattcaa
    20461 atattgaatc tgatcttctg cttcttgttt aaaagcttcc aattcgcttt cacgaacttg
    20521 tttactcatc tgttcgtcaa tgagcttcaa actttcatcc ctaactgtgt gaatttccgt
    20581 ttcagtcatt tcaaatatcg tcgaataaaa attgttcggc agtttttggt aaaacgcaac
    20641 gttttctgca ttcacgtttt caaaattttt cttcagtgta gcaatccgct cttctgtagt
    20701 aggtttcggg atattttcat cttccttcgc cttatctacc ttgccattat aagatttgtt
    20761 gatggtatca ttcgtcttat cgatcaattc gaataattgc ttgatccgat tgttctggtc
    20821 atctgccaga tctttttgat aagtatattc tggagtcact gcttctgctg ccagttttct
    20881 tctttgttcg gtttcttccg tgttctcaat cgttttattc gcacggatac tctcttctgc
    20941 tacttggcct tctctgaaat caatggcctt ttgtttgaca ctactaaatg taacgccgca
    21001 gacaatgata aaaaacaaga acagaaggaa aggcaatgct gcctttccga gtttttcttg
    21061 tagtttctgc agtgttttat tcaacatgtg cttatcctcc taagcaaagc ttattcttga
    21121 tgaagatccg ccttttcata agctttgatg atttcggcaa ctacagggtg ccgaacaacg
    21181 tcacttgctt caaaattaac aaaatcgatt tttttgattt gctttaatgt tcgttctgca
    21241 tgaacaagac cacttgtcgt cccacgaggc aagtctatct gacttgtatc cccattgaca
    21301 atcatttttg attgataacc gagacgggtc aagaacattt tcatctgagc gactgtcgtg
    21361 ttttgtgctt cgtccaagat aacgaatgca tcatccaatg tccgcccccg catgtaagct
    21421 aaaggtgcga tttcgatcac gcctcgttcc atcaatcggt tcgtatgatc caatccaaaa
    21481 atttggtata aagcatcata aacaggccgc agataaggat ccactttttc tttcagatcc
    21541 ccgggtagaa agcctaggtt ttcccctgcc tccacagcgg gccgcgtcaa aatgattttt
    21601 tgcacttcgc ctttttttag ggcagcaatt gctaatacta cggcaagaaa cgttttccct
    21661 gttccggctg gccctacacc aaatacgatg tcatgtttgg ctaccgattc aacgtatttc
    21721 ttttgaccgc tgttttttac ccgtataggt tttccattac ggtctttgac gatctcttcc
    21781 tcatacatct caacaaaata atctaatgta cctttttgcc ccattttcaa tgccgtcaca
    21841 acatctggcg tacttacgtg gattcctcgt ttgatcaatt cctgtaatgt ccgaatgact
    21901 gaggcagcca aggaaatctg ggattgctca ccaatgatct gaatgatttc tccacgtgta
    21961 tggatggttg tttccgttgt ttcctcgatc agttttatat gcttgtcgtg tgcacctaaa
    22021 agcatgctga catcatcagc agctgtcaat ctaatctcta aagaactaga ctcttccgtc
    22081 aaaaatagac atcccctttt tttcgattca ttctttccta ttttaccata ctcagaagaa
    22141 actatatagc aaataagaaa aggttgtaaa agagtcgctt aaccatacgt accaagaaac
    22201 gttaaaaaat agccgtttcc attcgctaaa ggtctcgttt tgataaaaag aagctgagaa
    22261 tgtaagttat atgaaagtgg agaacaaaat atttcgtttc attcaacaag tattctctag
    22321 gtatttttcc tgtttcaaaa aaagaaaaca aaatagttaa aatattcttg catttcaaaa
    22381 aaaacaggtg tattattacg tcaacaaata aacatacagc aaagcgtcga ggaaaacagt
    22441 aggctattca acgatttatt cccagtgagt catgtaagtg agaaatgaca ataaatcgaa
    22501 tagacgaaca tcatttccga gtcaatgtcg aaaacgtaga tcattgtggc tgcctccatt
    22561 atcaggctag cggataactt tatcaaaaag cgtccgtgaa tgaagaagct tttttaagcg
    22621 aaaaaaggtg gtaccgttct agaatctaga gcccttttcc caagtggaaa agggcttttt
    22681 tttattttaa ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca
    22741 acgtttatat cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa
    22801 aacttattca tggaaacaac tattgatggt cagttcactt atcttcggga tgttcttcgg
    22861 gtcaggtaat ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag
    22921 tgctggcgta ggatttgcta tctcaggtgc tcttttccca ttactagcta tcttagctgt
    22981 agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc
    23041 tgccttgttc ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac
    23101 agctgctact gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt
    23161 aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc
    23221 aacacggttg gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc
    23281 aatcattttt gtcgtttctt tattccatcc aatgggaaat ctaaatcaat ctgctgcaga
    23341 tacttatcaa acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc
    23401 agtagctttg cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa
    23461 agataaaaaa gtcacagaat taacggcaaa agctggtagc ctgagcgttg cgctagaagt
    23521 catgatctat tttggacttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc
    23581 agaaaatggc ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc
    23641 tagcttctta ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt
    23701 ttcttttgcg caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg
    23761 gttaacgact ttcgtctctt ttgtcgttgc aaatgctggt ttagacaata tcatccaatg
    23821 gtctttacca gtattgatgc ttttgtatcc attgtcgctt gcgttgatct tgctttcctt
    23881 aacagcaaaa tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc
    23941 tgttcctgcc gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt
    24001 tgtagcaagc atgctcgaat tttatcatca tcacgttcct tttgctgcat taggacttgg
    24061 ctggatggtc ccaacgttac ttggttacgc tggcagccta ctgttttatt acgcctatcg
    24121 cctatcaggg tataaacaag aagcaaatga actaccagaa gaataaccaa atcaaacaca
    24181 atttaacata agaaatatta aacaggaaaa aaccagctag atgaaagaat cctattcttt
    24241 cgtctagcta ttttttagtt ctctttttta cgcgcacaaa aaaactgcgg acaatgattg
    24301 tccgcagttt aaatgggctc tataatatct agtgttggtg acgccttctg ttgccaatac
    24361 tttttctttt ttctaataaa cgcaaacaga caccttgaaa tcctttagaa taaagtcgac
    24421 gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta
    24481 gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga gcgaatcaat
    24541 gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccagagga atgcttccat
    24601 tgtcactatc aaaataagca aacgatcatt aaat
//
LOCUS       DAZKCC010000038        24337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000038 DAZKCC010000000
VERSION     DAZKCC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.038"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..2477)
                     /locus_tag="ACG8VH_001575"
     CDS             complement(132..2477)
                     /locus_tag="ACG8VH_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP9110132.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="ACG8VH_001576"
     CDS             2756..3658
                     /locus_tag="ACG8VH_001576"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9110133.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="ACG8VH_001577"
     CDS             3869..5644
                     /locus_tag="ACG8VH_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP9110134.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="ACG8VH_001578"
     CDS             complement(5710..7296)
                     /locus_tag="ACG8VH_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9110135.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="ACG8VH_001579"
     CDS             7547..7771
                     /locus_tag="ACG8VH_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP9110136.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="ACG8VH_001580"
     CDS             7913..9664
                     /locus_tag="ACG8VH_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP9110137.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="ACG8VH_001581"
     CDS             9664..11448
                     /locus_tag="ACG8VH_001581"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110138.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /gene="licT"
                     /locus_tag="ACG8VH_001582"
                     /pseudo
     CDS             11783..12635
                     /gene="licT"
                     /locus_tag="ACG8VH_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12732..14618
                     /locus_tag="ACG8VH_001583"
     CDS             12732..14618
                     /locus_tag="ACG8VH_001583"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9110139.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="ACG8VH_001584"
     CDS             complement(14659..15561)
                     /locus_tag="ACG8VH_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9110140.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="ACG8VH_001585"
     CDS             15791..17194
                     /locus_tag="ACG8VH_001585"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9110141.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17194..18627
                     /locus_tag="ACG8VH_001586"
     CDS             17194..18627
                     /locus_tag="ACG8VH_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9110142.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="ACG8VH_001587"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="ACG8VH_001587"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9110143.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="ACG8VH_001588"
     CDS             19911..21638
                     /locus_tag="ACG8VH_001588"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9110144.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21654..22004
                     /locus_tag="ACG8VH_001589"
     CDS             21654..22004
                     /locus_tag="ACG8VH_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9110145.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="ACG8VH_001590"
     CDS             complement(22098..22958)
                     /locus_tag="ACG8VH_001590"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP9110146.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="ACG8VH_001591"
     CDS             23050..23478
                     /locus_tag="ACG8VH_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9110147.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /gene="rpsU"
                     /locus_tag="ACG8VH_001592"
     CDS             23640..23816
                     /gene="rpsU"
                     /locus_tag="ACG8VH_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9110148.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="ACG8VH_001593"
     CDS             23843..24289
                     /locus_tag="ACG8VH_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP9110149.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5186 g         7090 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaattc
//
LOCUS       DAZKCC010000039        23830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000039 DAZKCC010000000
VERSION     DAZKCC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.039"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..790)
                     /locus_tag="ACG8VH_001594"
     CDS             complement(56..790)
                     /locus_tag="ACG8VH_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9110150.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="ACG8VH_001595"
     CDS             1117..1527
                     /locus_tag="ACG8VH_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9110151.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="ACG8VH_001596"
     CDS             1520..2221
                     /locus_tag="ACG8VH_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP9110152.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="ACG8VH_001597"
     CDS             2221..3807
                     /locus_tag="ACG8VH_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110153.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="ACG8VH_001598"
     CDS             3826..4047
                     /locus_tag="ACG8VH_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP9110154.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="ACG8VH_001599"
     CDS             4044..4805
                     /locus_tag="ACG8VH_001599"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP9110155.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="ACG8VH_001600"
     CDS             5185..5694
                     /locus_tag="ACG8VH_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9110156.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="ACG8VH_001601"
     CDS             complement(5769..6308)
                     /locus_tag="ACG8VH_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP9110157.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ACG8VH_001602"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ACG8VH_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9110158.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="ACG8VH_001603"
     CDS             7321..8172
                     /locus_tag="ACG8VH_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9110159.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="ACG8VH_001604"
     CDS             8200..8835
                     /locus_tag="ACG8VH_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP9110160.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="ACG8VH_001605"
     CDS             8855..9628
                     /locus_tag="ACG8VH_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9110161.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="ACG8VH_001606"
     CDS             10036..10833
                     /locus_tag="ACG8VH_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP9110162.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="ACG8VH_001607"
     CDS             10811..11224
                     /locus_tag="ACG8VH_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9110163.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="ACG8VH_001608"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="ACG8VH_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9110164.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="ACG8VH_001609"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="ACG8VH_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9110165.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /locus_tag="ACG8VH_001610"
     CDS             16001..16561
                     /locus_tag="ACG8VH_001610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP9110166.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ACG8VH_001611"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ACG8VH_001611"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP9110167.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="ACG8VH_001612"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="ACG8VH_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP9110168.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="ACG8VH_001613"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="ACG8VH_001613"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9110169.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="ACG8VH_001614"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="ACG8VH_001614"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9110170.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="ACG8VH_001615"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="ACG8VH_001615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9110171.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="ACG8VH_001616"
     CDS             21908..22486
                     /locus_tag="ACG8VH_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9110172.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="ACG8VH_001617"
     CDS             22538..23020
                     /locus_tag="ACG8VH_001617"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP9110173.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="ACG8VH_001618"
     CDS             23137..23511
                     /locus_tag="ACG8VH_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110174.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23624..>23830)
                     /locus_tag="ACG8VH_001619"
     CDS             complement(23624..>23830)
                     /locus_tag="ACG8VH_001619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110175.1"
                     /translation="NLTTFENFRHKIFTTSDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7784 a         4286 c         5116 g         6644 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttatttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt
//
LOCUS       DAZKCC010000040        23726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000040 DAZKCC010000000
VERSION     DAZKCC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.040"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..190)
                     /locus_tag="ACG8VH_001620"
     CDS             complement(71..190)
                     /locus_tag="ACG8VH_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9110176.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            369..2030
                     /locus_tag="ACG8VH_001621"
     CDS             369..2030
                     /locus_tag="ACG8VH_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9110177.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2100..2447)
                     /locus_tag="ACG8VH_001622"
     CDS             complement(2100..2447)
                     /locus_tag="ACG8VH_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP9110178.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2531..3472
                     /gene="opp3b"
                     /locus_tag="ACG8VH_001623"
     CDS             2531..3472
                     /gene="opp3b"
                     /locus_tag="ACG8VH_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9110179.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3473..4516
                     /locus_tag="ACG8VH_001624"
     CDS             3473..4516
                     /locus_tag="ACG8VH_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9110180.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4522..5592
                     /locus_tag="ACG8VH_001625"
     CDS             4522..5592
                     /locus_tag="ACG8VH_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110181.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5582..6535
                     /locus_tag="ACG8VH_001626"
     CDS             5582..6535
                     /locus_tag="ACG8VH_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110182.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6547..7557
                     /locus_tag="ACG8VH_001627"
     CDS             6547..7557
                     /locus_tag="ACG8VH_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9110183.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7842..8663)
                     /locus_tag="ACG8VH_001628"
     CDS             complement(7842..8663)
                     /locus_tag="ACG8VH_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9110184.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8760..9038)
                     /locus_tag="ACG8VH_001629"
     CDS             complement(8760..9038)
                     /locus_tag="ACG8VH_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110185.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9306..11315
                     /gene="metG"
                     /locus_tag="ACG8VH_001630"
     CDS             9306..11315
                     /gene="metG"
                     /locus_tag="ACG8VH_001630"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9110186.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11794..12381)
                     /locus_tag="ACG8VH_001631"
     CDS             complement(11794..12381)
                     /locus_tag="ACG8VH_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110187.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12556..13878
                     /locus_tag="ACG8VH_001632"
     CDS             12556..13878
                     /locus_tag="ACG8VH_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9110188.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14033..14809
                     /locus_tag="ACG8VH_001633"
     CDS             14033..14809
                     /locus_tag="ACG8VH_001633"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9110189.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14802..15371
                     /gene="rnmV"
                     /locus_tag="ACG8VH_001634"
     CDS             14802..15371
                     /gene="rnmV"
                     /locus_tag="ACG8VH_001634"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP9110190.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15364..16248
                     /gene="rsmA"
                     /locus_tag="ACG8VH_001635"
     CDS             15364..16248
                     /gene="rsmA"
                     /locus_tag="ACG8VH_001635"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9110191.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16699..17328
                     /locus_tag="ACG8VH_001636"
     CDS             16699..17328
                     /locus_tag="ACG8VH_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110192.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17452..18561)
                     /locus_tag="ACG8VH_001637"
     CDS             complement(17452..18561)
                     /locus_tag="ACG8VH_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110193.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18770..19192
                     /gene="mgsA"
                     /locus_tag="ACG8VH_001638"
     CDS             18770..19192
                     /gene="mgsA"
                     /locus_tag="ACG8VH_001638"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9110194.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19379..19477
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19515..20051
                     /locus_tag="ACG8VH_001639"
     CDS             19515..20051
                     /locus_tag="ACG8VH_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9110195.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20091..21854)
                     /locus_tag="ACG8VH_001640"
     CDS             complement(20091..21854)
                     /locus_tag="ACG8VH_001640"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110196.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21857..23572)
                     /locus_tag="ACG8VH_001641"
     CDS             complement(21857..23572)
                     /locus_tag="ACG8VH_001641"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110197.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7646 a         4333 c         4811 g         6936 t
ORIGIN      
        1 aaaatggctt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
       61 caccgtttat tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat
      121 tcttttttat ctttgtatgg caataataga caatataacg aaaattattt aatggctttt
      181 ggaagaccat ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca
      241 cgaaaaaatc attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt
      301 cattaaactc ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg
      361 tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg
      421 ctggatgtta tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag
      481 atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag
      541 ttgcaacaga tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc
      601 ttgatgaaga cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag
      661 acggattaac ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg
      721 ttacagcagc ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg
      781 aatatgctta cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag
      841 ataaatcaga actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga
      901 aacaaacacc atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat
      961 ccgtagtgga agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg
     1021 gaccattcac attagaagat tttgatggac caggaacaga tacagaatgg acttataaga
     1081 aaaatgatca ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag
     1141 taaaagaatc ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct
     1201 tgtctggtga gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag
     1261 cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga
     1321 acctaagaaa agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag
     1381 gtgacggatc agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa
     1441 cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa
     1501 aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga
     1561 tggcttctga tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag
     1621 aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc
     1681 gctcaacttc gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc
     1741 caagcagctt tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca
     1801 atgcagaata tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag
     1861 cccgttggga tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc
     1921 cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc
     1981 atgcggcagg cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac
     2041 cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact
     2101 tatcctaaca aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg
     2161 atcagccaaa agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt
     2221 ttctttcggt tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc
     2281 gaagaaaaga cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga
     2341 tcattagata attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc
     2401 agaatcagta gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt
     2461 tatttttggt tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg
     2521 ggaaacatta gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat
     2581 cacgctttgg ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc
     2641 atatacgaat caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg
     2701 tttggataaa ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga
     2761 ttttggaatt tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat
     2821 cggaccatcc ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat
     2881 tttaggaacg atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt
     2941 agcgatatta ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt
     3001 tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt
     3061 accaacaatt gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga
     3121 aatggtggaa gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg
     3181 ttgggaaatc gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt
     3241 aggaccgtta gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat
     3301 tcctggtatc ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat
     3361 ggctgtaacg atcctttact catttatgct gatttttgtt attttgatcg tggatctatt
     3421 gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat
     3481 gataccaaag cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca
     3541 aaaaaatacc gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga
     3601 ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt
     3661 catcattttg atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa
     3721 cgtggcttat atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg
     3781 aaaagcaatg gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa
     3841 cgtaaatttc tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat
     3901 gggaatgaga atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg
     3961 tgtagcttac gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg
     4021 ttttttggaa atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt
     4081 ttttgaacca ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat
     4141 ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg
     4201 attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc
     4261 tagtgtcatt attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt
     4321 tttaagcttt atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag
     4381 cgaagggtac aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt
     4441 atcggttatc atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc
     4501 taaaatgaaa gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct
     4561 tttgatacgt atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa
     4621 ggagaaacat tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc
     4681 atcatgcgct tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt
     4741 aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg
     4801 atgattttcc aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc
     4861 gctgagtccc ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta
     4921 gaattgctta aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat
     4981 cagttctcag gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg
     5041 gaaatactga ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt
     5101 ttggagctgc tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat
     5161 gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc
     5221 gttgaagttg gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga
     5281 ctgcttggtt caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt
     5341 tctccaccgg atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc
     5401 gcgatgaaaa tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag
     5461 gcagcaactt ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa
     5521 cgtcgctggg ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct
     5581 gatgccaaat aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa
     5641 gaatgaagta catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg
     5701 tttagttgga gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa
     5761 tgaacctact gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa
     5821 aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc
     5881 gttgaatcct cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact
     5941 agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt
     6001 gaatccaagc cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat
     6061 tggtattgca cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc
     6121 tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga
     6181 acatcaattg acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga
     6241 ccgaatcggt gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta
     6301 taatcacggt gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc
     6361 agaccatgag agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt
     6421 acgtcaactt cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc
     6481 atattacgca aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg
     6541 acatagatgc taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa
     6601 ttgattcgta cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag
     6661 aaaagttggc aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa
     6721 gaatacccat tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg
     6781 gatcgggctt ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa
     6841 aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat
     6901 ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt
     6961 gaaggatcga ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca
     7021 ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc
     7081 tatcatccga agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt
     7141 caccagacag aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc
     7201 gctcaccata gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa
     7261 gtaatggatc tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct
     7321 gggaaattgg tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca
     7381 catgctgatt tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc
     7441 caatggtata ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc
     7501 gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa
     7561 atgaatgaaa ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt
     7621 ttgtttctag gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac
     7681 aatagcagaa aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt
     7741 ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt
     7801 tttttcttga atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc
     7861 ttgagctgat agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg
     7921 aaatattgga gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct
     7981 aagaatttag caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt
     8041 agaacttgcg gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt
     8101 tgactatggt caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc
     8161 gagatagcca cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac
     8221 cagcgtactt ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag
     8281 tagctgactg tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt
     8341 tgctcaatat tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg
     8401 ctttctccag ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga
     8461 taaggatgcc cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag
     8521 cacatcagta cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt
     8581 tgataccagt taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct
     8641 tctttagaag caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc
     8701 ttattgtagc agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt
     8761 taaatatgaa aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc
     8821 aatactttat accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca
     8881 atgataaaac gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta
     8941 aaataaccat acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa
     9001 gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa
     9061 gatagatagt ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag
     9121 atatgaaaca aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat
     9181 tgaacgtaga tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat
     9241 gcttttttta tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga
     9301 gccccgtggc agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca
     9361 aacttcatat cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac
     9421 ggttgatggg ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg
     9481 aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg
     9541 atgtcaagaa attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag
     9601 atgattacca taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata
     9661 tttacttagg tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa
     9721 cacaacttgc agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa
     9781 gtggacacga agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg
     9841 cagaccgtct attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta
     9901 aaaacgaaat gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa
     9961 caacttttac ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga
    10021 tcgatgcatt atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat
    10081 tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata
    10141 cgatctattg gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg
    10201 ggcatggctg gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt
    10261 atcctgaaat gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg
    10321 ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt
    10381 atgatctggc aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat
    10441 attgtgatgg actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt
    10501 ctacgacggc tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca
    10561 atacagcatt ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa
    10621 cagaaccatg ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga
    10681 tccatttagc tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa
    10741 cgccagagaa gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat
    10801 tacactttgg cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc
    10861 cacgtttaga tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc
    10921 aaacaaacga agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag
    10981 aaaaacaaat caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca
    11041 actgtcaaaa agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca
    11101 gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga
    11161 tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca
    11221 gtcaaggaat gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac
    11281 caaaatccat gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag
    11341 ttgtgtcata cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    11401 acttgaagcc aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa
    11461 gaagtacgag cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa
    11521 cagaaaaaaa gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct
    11581 tttttattgt ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta
    11641 tatctaacag tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt
    11701 tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc
    11761 gatgattgtt ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat
    11821 ttttgtcttc aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac
    11881 ataccagaaa atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg
    11941 taacaagatc aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat
    12001 gacttcacta agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt
    12061 catccgccag ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa
    12121 aataccgata ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat
    12181 cacaggacct ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg
    12241 tgataacgtt gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga
    12301 ctgctgttgt tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg
    12361 ataagaacgc ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata
    12421 cgaaaaataa aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa
    12481 aaaagaatgg taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga
    12541 agaaagagga tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta
    12601 gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact
    12661 gctagtactc agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg
    12721 atctgtgctg gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct
    12781 gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa
    12841 agcttgattt actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg
    12901 acgaacttat tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt
    12961 tctgtgacag ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg
    13021 ttgattgtta aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga
    13081 caaggaactg tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga
    13141 ttgagcagtg cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca
    13201 atggcagggg aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg
    13261 agttttgtaa cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt
    13321 gatcatcttg ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga
    13381 atcggtggaa agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt
    13441 tatacactaa ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc
    13501 agtaaacagc tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc
    13561 cagttagcag tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg
    13621 ttgatttttg tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga
    13681 aaaaaagcgc cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt
    13741 attgcgattc tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta
    13801 gcattgatcg gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa
    13861 aaacgctcag aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc
    13921 tagatcttcg tgctttttct atttcaataa aacaagttct tctattttga tttttgctac
    13981 ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt
    14041 tgattctcat acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca
    14101 gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat
    14161 tgaaaagtct ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca
    14221 tccgacagag gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac
    14281 tttaccaaag gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc
    14341 gaaagaagtc caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct
    14401 accaatcagt attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga
    14461 agatgttcga gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa
    14521 acgatttctt gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc
    14581 attagacgtc caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga
    14641 tgccccttat ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg
    14701 ttatacagta gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca
    14761 aacatggaca aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc
    14821 aagagattat agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg
    14881 cagatacgat agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac
    14941 atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa
    15001 taagaaaaat cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac
    15061 aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt
    15121 tagaagcatt gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca
    15181 ctagacaaga ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag
    15241 aaaaactagg tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt
    15301 tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac
    15361 aacatgagtg aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa
    15421 catggttttt catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta
    15481 aggaaaatcg tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca
    15541 ggaatcggtg cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag
    15601 atcgatgatc gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact
    15661 attgtacatc aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat
    15721 gaggagcttc cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg
    15781 atgcattttc ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa
    15841 gtagctgatc gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct
    15901 gttcaatatt atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct
    15961 cagccaaatg tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa
    16021 gtgacagacg aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa
    16081 acgctttgga ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta
    16141 gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta
    16201 caagaattcg ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg
    16261 tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    16321 gttcctcatg tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg
    16381 tttaagaaag aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa
    16441 aacagcttct catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac
    16501 tattatgccg aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt
    16561 gatcaaaaaa ttcttatact tgatactcac actcattttg tatggattta gaaaagaata
    16621 tgtgtcttga cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca
    16681 tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta
    16741 ttgattggga tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct
    16801 tcctttactt tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt
    16861 gcagtggctg ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc
    16921 gcattgcgag cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag
    16981 catccgagta tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg
    17041 ttcaattttg tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt
    17101 tactttattg gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc
    17161 atcttgatgg ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt
    17221 atagcaggaa cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa
    17281 aatcggaaag agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc
    17341 aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc
    17401 ctaaaaagag ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag
    17461 tttgatgact tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag
    17521 gtcgactatt tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt
    17581 agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc
    17641 tgatttcact acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc
    17701 tggacgaata ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt
    17761 ttttccttca ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa
    17821 tgtcacatta aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac
    17881 gggtgtatca taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat
    17941 aacgattcgg tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt
    18001 tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc
    18061 atctaaattt gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg
    18121 tcccaatgcc acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta
    18181 ttctgtcaga cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc
    18241 atatttacgt aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa
    18301 tgcgtagttt tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac
    18361 ttttccgccg atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa
    18421 agtagttgac tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat
    18481 tgatagatta aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt
    18541 tttcagagcc atttctacca taatacgttc cacactttct tttttagttt tattaactat
    18601 ttaaagtata aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct
    18661 tttttttgtc aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga
    18721 tacactggag taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc
    18781 tttgatcgct catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac
    18841 aatattggaa aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc
    18901 aaccggcttg ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg
    18961 agccatgatt tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc
    19021 acagccccat gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc
    19081 gttagctacg aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct
    19141 agattttcga agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag
    19201 cgtatggaaa aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg
    19261 ataatatgcg atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt
    19321 atttgcagta aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata
    19381 cagagtagaa aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg
    19441 acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa
    19501 aggagatgct aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct
    19561 tattgattgc attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta
    19621 aaatcagtgt cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt
    19681 cagggatagg cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat
    19741 tctttattgg atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct
    19801 atcgaaaaga gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca
    19861 tcaacttatt tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct
    19921 gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg
    19981 ccatttatta cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc
    20041 acgctaaata aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct
    20101 gtgaattgtg cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg
    20161 gtgcccattt cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt
    20221 gtggacaggc gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct
    20281 tgttggattt ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac
    20341 atagggggat tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat
    20401 atctttaacc cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat
    20461 tcatacatat aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc
    20521 ccaaaacgaa gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt
    20581 ccaaaacttt tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta
    20641 attgttcctt ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg
    20701 gcccctgttt ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct
    20761 tcgatcaaac gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct
    20821 ttgatatttt tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga
    20881 tcgatcaaat caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt
    20941 ccagaaagtt cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc
    21001 acaccaacag tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc
    21061 gataaataag caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat
    21121 tgtgcccgtt gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta
    21181 gcttcttctt gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag
    21241 cccgagatat ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca
    21301 acgatccaac tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt
    21361 ggacttagac gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat
    21421 tgattgaagg ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg
    21481 acgatatttc cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca
    21541 aatgcatctt ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg
    21601 atgagccatt gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg
    21661 aacagtcgat tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt
    21721 tgtaacgtca tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa
    21781 atgtcttttg cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat
    21841 ttttttactt tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg
    21901 attgagcaag taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta
    21961 agatatgatg tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc
    22021 caagataagt ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta
    22081 aagagtcatc taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc
    22141 gctgtttttg tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat
    22201 ttggaagctg acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt
    22261 tttctgtggc gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta
    22321 cagaagtttg gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa
    22381 catccattcc gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt
    22441 gtgtcaaagt cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct
    22501 tcagctgaaa tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa
    22561 catggtcaaa agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt
    22621 caagaatcga attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac
    22681 gtgtgaagat cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat
    22741 aattgatcaa agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc
    22801 caactgtcag caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata
    22861 gggcagagat attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga
    22921 acttctctgt ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat
    22981 tctgactaac gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa
    23041 cggttttttt gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa
    23101 acagacctac ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg
    23161 gtgctctgat caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt
    23221 ttgtcattcg ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag
    23281 tattgatttt tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg
    23341 aagcatagta ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc
    23401 ataagcccat ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg
    23461 aagccatcat taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc
    23521 ccaaaatgat ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc
    23581 tcctattatt tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa
    23641 atttagaatg aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt
    23701 tagaaattga atagactatt tttttt
//
LOCUS       DAZKCC010000041        23645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000041 DAZKCC010000000
VERSION     DAZKCC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.041"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8VH_001642"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8VH_001642"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9110198.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACG8VH_001643"
     CDS             complement(1174..1869)
                     /locus_tag="ACG8VH_001643"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP9110199.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACG8VH_001644"
     CDS             complement(1882..2598)
                     /locus_tag="ACG8VH_001644"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFP9110200.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACG8VH_001645"
     CDS             2928..3536
                     /locus_tag="ACG8VH_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP9110201.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACG8VH_001646"
     CDS             3861..4184
                     /locus_tag="ACG8VH_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP9110202.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACG8VH_001647"
     CDS             4366..5736
                     /locus_tag="ACG8VH_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP9110203.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACG8VH_001648"
     CDS             complement(5866..7167)
                     /locus_tag="ACG8VH_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9110204.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACG8VH_001649"
     CDS             complement(7521..9512)
                     /locus_tag="ACG8VH_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP9110205.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACG8VH_001650"
     CDS             complement(9656..9922)
                     /locus_tag="ACG8VH_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9110206.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG8VH_001651"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG8VH_001651"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9110207.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG8VH_001652"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG8VH_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9110208.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACG8VH_001653"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACG8VH_001653"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9110209.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACG8VH_001654"
     CDS             complement(14082..14603)
                     /locus_tag="ACG8VH_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP9110210.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACG8VH_001655"
     CDS             complement(14606..15448)
                     /locus_tag="ACG8VH_001655"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP9110211.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACG8VH_001656"
     CDS             complement(15441..16166)
                     /locus_tag="ACG8VH_001656"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP9110212.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACG8VH_001657"
     CDS             16342..16638
                     /locus_tag="ACG8VH_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110213.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACG8VH_001658"
     CDS             17206..18798
                     /locus_tag="ACG8VH_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9110214.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACG8VH_001659"
     CDS             18811..19551
                     /locus_tag="ACG8VH_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9110215.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="ACG8VH_001660"
     CDS             19719..21068
                     /locus_tag="ACG8VH_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9110216.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="ACG8VH_001661"
     CDS             complement(21196..21786)
                     /locus_tag="ACG8VH_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9110217.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG8VH_001662"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG8VH_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9110218.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6771 a         4761 c         4503 g         7610 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
    23641 aaaaa
//
LOCUS       DAZKCC010000042        22852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000042 DAZKCC010000000
VERSION     DAZKCC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.042"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG8VH_001663"
     CDS             3..371
                     /locus_tag="ACG8VH_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9110219.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG8VH_001664"
     CDS             551..1492
                     /locus_tag="ACG8VH_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9110220.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG8VH_001665"
     CDS             1561..2292
                     /locus_tag="ACG8VH_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9110221.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG8VH_001666"
     CDS             2436..4616
                     /locus_tag="ACG8VH_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9110222.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG8VH_001667"
     CDS             4636..5118
                     /locus_tag="ACG8VH_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP9110223.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG8VH_001668"
     CDS             5134..5895
                     /locus_tag="ACG8VH_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9110224.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG8VH_001669"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG8VH_001669"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9110225.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG8VH_001670"
     CDS             7667..8152
                     /locus_tag="ACG8VH_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP9110226.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG8VH_001671"
     CDS             8145..8828
                     /locus_tag="ACG8VH_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9110227.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG8VH_001672"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG8VH_001672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9110228.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8VH_001673"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8VH_001673"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP9110229.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8VH_001674"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8VH_001674"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9110230.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG8VH_001675"
     CDS             13125..13457
                     /locus_tag="ACG8VH_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9110231.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8VH_001676"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8VH_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9110232.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG8VH_001677"
     CDS             14283..15656
                     /locus_tag="ACG8VH_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9110233.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8VH_001678"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8VH_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP9110234.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG8VH_001679"
     CDS             16711..17151
                     /locus_tag="ACG8VH_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9110235.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG8VH_001680"
     CDS             17295..17996
                     /locus_tag="ACG8VH_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP9110236.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG8VH_001681"
     CDS             17993..19114
                     /locus_tag="ACG8VH_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP9110237.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG8VH_001682"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG8VH_001682"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9110238.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG8VH_001683"
     CDS             20493..21401
                     /locus_tag="ACG8VH_001683"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9110239.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG8VH_001684"
     CDS             21436..21708
                     /locus_tag="ACG8VH_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP9110240.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG8VH_001685"
     CDS             21719..22765
                     /locus_tag="ACG8VH_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP9110241.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7700 a         3984 c         5015 g         6153 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt at
//
LOCUS       DAZKCC010000043        22719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000043 DAZKCC010000000
VERSION     DAZKCC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.043"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG8VH_001686"
     CDS             complement(11..1942)
                     /locus_tag="ACG8VH_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9110242.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8VH_001687"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8VH_001687"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9110243.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG8VH_001688"
     CDS             complement(2871..3623)
                     /locus_tag="ACG8VH_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9110244.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG8VH_001689"
     CDS             complement(3782..3991)
                     /locus_tag="ACG8VH_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110245.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG8VH_001690"
     CDS             complement(4002..5330)
                     /locus_tag="ACG8VH_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110246.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG8VH_001691"
     CDS             5466..5621
                     /locus_tag="ACG8VH_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP9110247.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8VH_001692"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8VH_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9110248.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8VH_001693"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8VH_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP9110249.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8VH_001694"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8VH_001694"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9110250.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG8VH_001695"
     CDS             complement(9305..10237)
                     /locus_tag="ACG8VH_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110251.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG8VH_001696"
     CDS             complement(10394..11053)
                     /locus_tag="ACG8VH_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9110252.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8VH_001697"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8VH_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9110253.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG8VH_001698"
     CDS             complement(12094..12384)
                     /locus_tag="ACG8VH_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110254.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8VH_001699"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8VH_001699"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9110255.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8VH_001700"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8VH_001700"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9110256.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG8VH_001701"
     CDS             complement(16049..17218)
                     /locus_tag="ACG8VH_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP9110257.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8VH_001702"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8VH_001702"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP9110258.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8VH_001703"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8VH_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9110259.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG8VH_001704"
     CDS             22182..22703
                     /locus_tag="ACG8VH_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP9110260.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAZKCC010000044        22628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000044 DAZKCC010000000
VERSION     DAZKCC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.044"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..334)
                     /locus_tag="ACG8VH_001705"
     CDS             complement(<1..334)
                     /locus_tag="ACG8VH_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFP9110261.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEI"
     gene            complement(425..877)
                     /locus_tag="ACG8VH_001706"
     CDS             complement(425..877)
                     /locus_tag="ACG8VH_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9110262.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(890..1300)
                     /locus_tag="ACG8VH_001707"
     CDS             complement(890..1300)
                     /locus_tag="ACG8VH_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9110263.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1575..1787
                     /locus_tag="ACG8VH_001708"
     CDS             1575..1787
                     /locus_tag="ACG8VH_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFP9110264.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1790..2002
                     /locus_tag="ACG8VH_001709"
     CDS             1790..2002
                     /locus_tag="ACG8VH_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110265.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2142..2297
                     /locus_tag="ACG8VH_001710"
     CDS             2142..2297
                     /locus_tag="ACG8VH_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110266.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2294..2518)
                     /locus_tag="ACG8VH_001711"
     CDS             complement(2294..2518)
                     /locus_tag="ACG8VH_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9110267.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2596..2784
                     /locus_tag="ACG8VH_001712"
     CDS             2596..2784
                     /locus_tag="ACG8VH_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110268.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2781..2954
                     /locus_tag="ACG8VH_001713"
     CDS             2781..2954
                     /locus_tag="ACG8VH_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110269.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2923..3051
                     /locus_tag="ACG8VH_001714"
     CDS             2923..3051
                     /locus_tag="ACG8VH_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110270.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            3110..3445
                     /locus_tag="ACG8VH_001715"
     CDS             3110..3445
                     /locus_tag="ACG8VH_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110271.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3678..4619
                     /locus_tag="ACG8VH_001716"
     CDS             3678..4619
                     /locus_tag="ACG8VH_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFP9110272.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4621..5511
                     /locus_tag="ACG8VH_001717"
     CDS             4621..5511
                     /locus_tag="ACG8VH_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFP9110273.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5554..6354
                     /locus_tag="ACG8VH_001718"
     CDS             5554..6354
                     /locus_tag="ACG8VH_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9110274.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6369..7220
                     /locus_tag="ACG8VH_001719"
     CDS             6369..7220
                     /locus_tag="ACG8VH_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9110275.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7286..7420
                     /locus_tag="ACG8VH_001720"
     CDS             7286..7420
                     /locus_tag="ACG8VH_001720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110276.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7421..7882
                     /locus_tag="ACG8VH_001721"
     CDS             7421..7882
                     /locus_tag="ACG8VH_001721"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9110277.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7908..8360
                     /locus_tag="ACG8VH_001722"
     CDS             7908..8360
                     /locus_tag="ACG8VH_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110278.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8466..8660
                     /locus_tag="ACG8VH_001723"
     CDS             8466..8660
                     /locus_tag="ACG8VH_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110279.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8691..8984
                     /locus_tag="ACG8VH_001724"
     CDS             8691..8984
                     /locus_tag="ACG8VH_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110280.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8915..9454
                     /locus_tag="ACG8VH_001725"
     CDS             8915..9454
                     /locus_tag="ACG8VH_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9110281.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9529..9996
                     /locus_tag="ACG8VH_001726"
     CDS             9529..9996
                     /locus_tag="ACG8VH_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFP9110282.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10743..10973
                     /locus_tag="ACG8VH_001727"
     CDS             10743..10973
                     /locus_tag="ACG8VH_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110283.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11132..11965
                     /gene="terS"
                     /locus_tag="ACG8VH_001728"
     CDS             11132..11965
                     /gene="terS"
                     /locus_tag="ACG8VH_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP9110284.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11958..13373
                     /locus_tag="ACG8VH_001729"
     CDS             11958..13373
                     /locus_tag="ACG8VH_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9110285.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13385..14914
                     /locus_tag="ACG8VH_001730"
     CDS             13385..14914
                     /locus_tag="ACG8VH_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9110286.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14832..15884
                     /locus_tag="ACG8VH_001731"
     CDS             14832..15884
                     /locus_tag="ACG8VH_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFP9110287.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15886..16203
                     /locus_tag="ACG8VH_001732"
     CDS             15886..16203
                     /locus_tag="ACG8VH_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110288.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16330..16953
                     /locus_tag="ACG8VH_001733"
     CDS             16330..16953
                     /locus_tag="ACG8VH_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFP9110289.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16968..17858
                     /locus_tag="ACG8VH_001734"
     CDS             16968..17858
                     /locus_tag="ACG8VH_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFP9110290.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17881..18096
                     /locus_tag="ACG8VH_001735"
     CDS             17881..18096
                     /locus_tag="ACG8VH_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110291.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18108..18449
                     /locus_tag="ACG8VH_001736"
     CDS             18108..18449
                     /locus_tag="ACG8VH_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110292.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18449..18820
                     /locus_tag="ACG8VH_001737"
     CDS             18449..18820
                     /locus_tag="ACG8VH_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110293.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18813..19208
                     /locus_tag="ACG8VH_001738"
     CDS             18813..19208
                     /locus_tag="ACG8VH_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFP9110294.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19210..19587
                     /locus_tag="ACG8VH_001739"
     CDS             19210..19587
                     /locus_tag="ACG8VH_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFP9110295.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19588..20196
                     /locus_tag="ACG8VH_001740"
     CDS             19588..20196
                     /locus_tag="ACG8VH_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP9110296.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20196..20537
                     /locus_tag="ACG8VH_001741"
     CDS             20196..20537
                     /locus_tag="ACG8VH_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110297.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20779..>22628
                     /locus_tag="ACG8VH_001742"
     CDS             20779..>22628
                     /locus_tag="ACG8VH_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP9110298.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTN"
BASE COUNT         8111 a         3318 c         4619 g         6580 t
ORIGIN      
        1 ttatttcaga agatgaagtt tcttctatat ttgaaacgat aagtgaagaa tagacatttt
       61 ttaatgtttc atcatacttt tgatttattt catcatttaa atcttctttc tcttttttta
      121 ataaaataat atcatttttt aacatagtaa tttgtttatt taaatctgat agtgtttctt
      181 ccttacttaa tataaatctt tctctatcat gtaatttatt tagtttttca tcattttttt
      241 gttctaattc ataagcttct ttttttattg tgttagcttt cgacagttta gtgattccta
      301 taattaataa aataagtcca atcaataata acattcaaaa ctcctcattt ctttgatata
      361 attattgtag agtttactca gaaatgagga aagagtccat gtgaaagcat gggctttttt
      421 atttttaatg ttgtaatact aaatgttcaa gattatgagg aagccctaaa tattcaataa
      481 tttggaactg actcatatca gataaatctt gttcatctat tgttctagtt aacatttcaa
      541 ttgcaaacat attagcttca gcttctatac cataaatcat acaactaagg ttgtttgctc
      601 taaaagctgc tgtgcaaata cctttatgga gtctaacgtg ggctatttca tgccataata
      661 caaatctttt catagaaggt gataggtttt gattaattaa tatcacataa catcttttat
      721 tgtgtatcgt ttgtcctaat gttttattcc ctaaatcagt ttcaattaaa tctatatcag
      781 aatatttgat tattttatat ggattaatcg catcagatcc aaactctaca aataattttt
      841 ctatttcttc taatatccag tatttgttta aatgcatacc aaagcccacc tattgacgat
      901 attttttagg agtgaatttt ttctttgctt taactttggt taaggcaagt gcaagttgaa
      961 tggcatcgta taatttttcc ttatcttcat cgtctaaagg ttcaccataa taatttgttt
     1021 cagcatctga attcaagcct tcaagtaatt tttcagcttg taaagcaata tcttgtttat
     1081 ctttttctgt taaatcataa taatggcgtt tatcagtacg tcctaaaaga taatcagttg
     1141 atacattaaa gtaatctgca actttttgta ttttatctcc agatggagta cttttattcc
     1201 atctagacat gcttccattt ccgaaattta actttctttc aagttctgcc aacgtcattg
     1261 aacgttcttt taataagatt tttatacggt ctaatagcat aggatatgca ttcctttcaa
     1321 aataccgaca aaaaaagtat ttttctgcaa attatctatt tacaaataga gaatttgcag
     1381 ttatactagt tttgtaagca agatatttac ggcatacaaa gactaaaagc aataaactaa
     1441 tagtctcccc agacatcagt aagctcgctt tttgtgcgct gtattttgta tgcttacata
     1501 atagcatatt tgcagttttt gtcaaactta ttagacaaaa atattagaaa atattcgata
     1561 agggggtatg aagcatgact ttaaaacagc aagtattaat tgctttagta aagaaaggtt
     1621 ggagtcaaag agagttagct cgacgaatgg gtatatcaat tacttattta cgagatatct
     1681 tcatcgaaaa aagaaaacct aaggaacgtt taaaacaaat cgaagaactt ttggatatca
     1741 agttagaagt agattctaat gatcagccat cagaacagga ggcgtaaaaa tggtacaacg
     1801 tttgagcaaa gcggagttta aagagaaata taagtattct gaaagtactt atcaaagaag
     1861 aatgaaggaa tttaaaaaat cacgttttag tgaggggtat aacgcagtta cttccaaaga
     1921 aataacaatt gatgtcgatt tatatgaaca gtttagaaca tggcgttcta aaaatcgatt
     1981 tagagttaga gctgtttgtt aatagctaaa aaatctaaat tcaccacttc tgaaaagagg
     2041 agttgctgct ctcctttcaa gtttgccaaa gtccacgttt tttcattttg gcaactcctc
     2101 ttcttggagg tggtgacggc gagagacgaa ggagggatga catgaaggaa gaatcaaaaa
     2161 tagggatttt gaatgagaac aattacacaa gattaattat tgaaacagaa gaagggaaaa
     2221 aagtagcaga agttacattg accgatgcaa ctcctgctga cggttatcgt attcgattaa
     2281 ctcctaaata tgattaacct tttggtgggt gtggatcgtt tccgtgacta tcacggcttt
     2341 gaatttttcc atctctgcca tgaataaata gctcagatcc ttgattttta gaaatctctc
     2401 tagcgatttt tactgcttct gattttgtat tagtatgtgc agtcgcttta gaatttccag
     2461 cacctttgac attccaacca ccattggaat taggtacaac atgttgattt tttcccatag
     2521 ttttcacctc catttctata gaaatatttt atcaaataga aatggagaaa ttcaataaac
     2581 gaaaggagaa gtgatatggc atataccttg caacaagaac atcaaattct cggtttgatt
     2641 aaacaacgca ggaaacaatt acaagatgac cgtgcagcgc ttagaaaagc cgatgagcta
     2701 tcagataggc aagctgaact aattgcttct gaacttgagg atttgagaat gctagaaata
     2761 aaaaataggg agattagatt atgaagaaga cagacacact ttttatagga ttcattttgg
     2821 ggttattagt gattgtagcg caccaaagta ttatcggggg aagcttgttc gcagcattga
     2881 tggttttaat caatctgctt gattcaaaag aaaggagcaa ctatggcacg agaagaagcg
     2941 ctaaaaatcg gtaaagtgat tgctgataat cggtggggaa atagccgccc tattatttta
     3001 agcaagcaac atatcgaaaa gcaaaaagca tggcaacaaa taaaaaagtg actctgccag
     3061 ccagcaaaga gtcacataca aatttataaa actaggagaa ttatatcaca tgaataagaa
     3121 aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg tttctattat
     3181 ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga caacagaaat
     3241 tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat tgtcagcaca
     3301 tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag aagaaaatca
     3361 accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg tgatgactcg
     3421 tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact cattatttta
     3481 tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt attcgcaatt
     3541 accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga acgagttagc
     3601 aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg aagcaccaat
     3661 tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa gaatggcttg
     3721 aagaccgtaa gaaaggcatc ggaggttcgg atgttggaac aattttagga ttgaataaat
     3781 ggaaatctcc ttatcaacta tggctagaaa aaacaggaca agtagtactt gaagaatcag
     3841 gaagtgagcc cgcttattgg ggtaatgttt tagaagaagt ggttgctaaa gagtttcagg
     3901 aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca ttacatccat
     3961 ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg gaatgcaaaa
     4021 cagccaatca atttctcggt aaagaatggg aaggcgaaga ggtaccgctt agctatctct
     4081 gtcaagttca acattacatg aacgttctaa acaaagacta ttgttacatc gctgtcttaa
     4141 tcggtggtca aaaatttatc tggaagcgga ttgaacgaga tcaagagctg atcgatacaa
     4201 tcactgaaca attagtagag ttttgggaaa caaacgttct tggaggtatc gagccaatta
     4261 ttgatggtag tcaagcgaca gctgatttct tgaaagagaa gtatgcagat gttgaagacg
     4321 ttcaaacgac gttaccaatt catttcgatg aactagttga acagaaaaac gaactcaaac
     4381 ggactaagaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt atcagcgagc
     4441 taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt tggaaatttg
     4501 tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat gtcgcaaatg
     4561 atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaagggag atcaaataat
     4621 atggcaacaa atgaatcgtt aaaaaatcaa ttggcagcaa agccacagaa acaagttgca
     4681 ccaggtcaat taggacttaa agctttgatg aatacaccaa caatgagaaa gaagttcgaa
     4741 gaggtgcttc atgacaatgc taatgctttt atgtcgaatg ttatgactct tgtatctaat
     4801 gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt aactgctgca
     4861 acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc attcaatagt
     4921 aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg ctataaagga
     4981 tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat cgaagtttac
     5041 gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga tccaaatggt
     5101 agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa tggattcaaa
     5161 aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa caataaagat
     5221 cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat ggcacgaaaa
     5281 actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat gcaagaagcc
     5341 accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat tgctgaaaca
     5401 gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga agaaaatgaa
     5461 gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata atgagggagc
     5521 tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt caaattacat
     5581 cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt atggaacttg
     5641 tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa agagatacaa
     5701 gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat gaacgaaggc
     5761 gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa acaatttgaa
     5821 aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc aataaaaaac
     5881 tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac aagtgaacaa
     5941 caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa taaaacatca
     6001 tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta cattctcgcg
     6061 gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa tctgcaaagt
     6121 tgggcagatg acattcggaa aatgatcgag atcgacaagc ggaccgaaaa ccaggtacgg
     6181 ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt atctgctaaa
     6241 aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta taaaacaaaa
     6301 caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat ttgagaggag
     6361 gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa gtgcttgtcc
     6421 aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg aagaaccctg
     6481 atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca ttgcgagttc
     6541 aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga gcaatcaatt
     6601 ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca ttgcagaatt
     6661 atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc tttgttaatt
     6721 cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga acaggtaaaa
     6781 gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac gataaaaaaa
     6841 tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac aacaatcctg
     6901 aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg gatttagtca
     6961 tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga gcgtttggca
     7021 taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt atcacaacga
     7081 acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca agaatgtttg
     7141 tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc ataaaaccag
     7201 tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc gtcagataat
     7261 gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga gtcgaatggt
     7321 tcggaagata agtaagctaa ttatttatcg ggatacagaa agatttctca ctgttttagg
     7381 taacgtctac gagaatccag agttattgga ggtagagtaa ttgccaaaaa tattagatgc
     7441 atgttgcggt agtagattgt tctggtttga taagaacaat tctgacgtaa catttatgga
     7501 ttgtcgtcag cagtatgaag aattatcaac aggtcatgtg atcaatgttg atccagatgt
     7561 ggtcgcagac tttcgcgata tgccttttga agatgatgtg ttcgatatgg ttgtatttga
     7621 cccgccacac ttaagacatg tcggcgaaaa ctcatggctt gccaaaaaat atggaaaatt
     7681 agacgaactt tggccagaag acatcaggca aggttttgca gagtgtatgc gagttttacg
     7741 accttcagga acattaattt ttaagtggaa cgaggaacag ataccacttt ctgatgtgtt
     7801 agaggctatt ggagaacagc cattgtttgg taataaacga agcaaaacac attggcttgt
     7861 gtttatgaaa aaattcagct aacggaggaa acaaaggagg aaaagtaatg aagattgtat
     7921 taaataaatg ttttggtggc tttgggttga gccatgaagc aaaaatggaa atttttaaaa
     7981 gaaaaaatat tgaagtattc ccatacatca ataactttag ctatgactca gatgatgaat
     8041 acacgagata cacaggccaa aagctaggtt cgatggattt tatctactat ttcaaaaaag
     8101 acccgaaaat cgacaaagtt acaggaattt atagcgaaat tgaccgactg tacggaattg
     8161 ctgatgattc cagtttttca agtgattcaa acagaggtga taaggacctc gttgctgttg
     8221 tcgaaaaact aggaagtgag gccagtggac catatgcaag tcttaaagtc gttgatatac
     8281 ccgatggggc tgagtgggaa ataagtgatt atgacggcgt tgaaactgca cattatggtt
     8341 ttcaaacagg tagtatttag tccactaatg acaactaaag aggaggcaga aggatgagtg
     8401 aagatgaata ctttgaacag tagtctatta acgacagaga tttttaactg gaaaggggtg
     8461 tagtcatgga cgcatcggaa gtagtaaata aaattaatag tctcaaagag gttttcggag
     8521 acgtcgaagt agtgataaac gcacaaggct atgacaacga attttttaag cagattaccg
     8581 acattagcat tcagcaagga atggaagacg aagacggaaa ctttatcgat gaagtagcca
     8641 ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg aggaagcgga atgaaactaa
     8701 aagacggatt ttactccagc ggccacggca tcggcggttt aatgctagat atgccgacaa
     8761 agaatcctaa aacacgtaag aaaccaaaat tcaaagtcgg tgacatggtt cgctgcgaag
     8821 cagaaggatt catctatcca tttcgtggat atgtagaaca cgtctataat cactcagcaa
     8881 tcattcgcat tgaaaacacg atggaatgtg ataagtggtt agcgaaaagc aaagagaatt
     8941 tagcagtggt gagattggtg gacatggaag ttataaaatc ttaattttgc ttttttacaa
     9001 ctatcctaca acaatgagcg ttattccaca actggaagga gaagaggcaa ttgtgaacat
     9061 tttagagaat atagatatta agcaaacaag gaaaaatgct agacgattac taaaaagata
     9121 cagaaattta gaacgtttgg tcggaccagt gaaaatagac ttttccgtga tgactgttac
     9181 caaaaatttg aaattcacaa ttgacagtca aaacgaagaa atcattgaag cgataagtac
     9241 tcgagattcg gttatcgaag cattaacacg gctaagtaga atccatttcc aggtacttta
     9301 ttatagttat tgttttccta ataagatgtc aatgtatcaa ataggagaaa aattaggata
     9361 ttccgataga actatcgaga gaatgaaggc agtagctttg gttgaatttg ctgaggcgta
     9421 taaatcagga gaactcattt cacgtacaaa ataaaaaaag tcggattcct ccgactatga
     9481 ataatatttc cgacataagt attatatcat aattggggga atcagaggat ggtacttttc
     9541 gatgtaaaga aatatgagac accaagcgcg aaagatgttg atatggaacg cacaaaacat
     9601 aatgtcgctg tgtttctttc agcatatcta tcagctagat gtagagtagg gcaacatcga
     9661 gaaccaaaag tgacagcatc ttattccttg gttccacctt ctacagctga tcatatattt
     9721 gaagcagaaa gaatgctgat cgataaagaa gaagcacagg aagaatttga gtatctgcat
     9781 aaattgtttg ttcgaggcta ttcagcgata cagcatccgc ataagcctga tgtgactgaa
     9841 aggcgcaaga agatattcta tgatcgttat atcaatggtc tgcccattta tgtaactgct
     9901 caaaggaata atactagcga agaatcggtt aaagtagaat caaacagaat tatcatccaa
     9961 tttgcttcat cgttagaact ggttgctttc aagtagccag tttttacact ttttataccc
    10021 ttttattacc aatttggttt ccattttata ccttttttat accaatcact tacctattca
    10081 acgttgtatt atgatagtgt cgaaaaatta ggaaacagga tcgacaaaat aaaatgtaag
    10141 ggaggaaatc tccctcatcg tttaattaag cttcgatagg cagcaacgga aatattaaga
    10201 ataaggatgt gaatttcaac tccttctaaa ttgttcttat tatctatcat ccgttgctgt
    10261 ctattgtttt tttaattatt cacacgataa ctaaaggtaa gtgaagagaa atgattccat
    10321 taataatttc aatttttgcg ctttgtctta atgtatatat gattggattt aaaaatgggc
    10381 aaaataaaaa atagtagcag ccaagaataa ttttatagtg tcactgtggc ggaaagggta
    10441 gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca tgcaaggttc gattccttgc
    10501 cagtgacttt ggcaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
    10561 cgcgttttgt gcatcgtgta ggttgctatt acataactgg ttaggttaga ttgagatttg
    10621 ggattcggta caaatgaatc gtcaaatgtc tcaagcacag gatcggaaac gtccctgcct
    10681 gtgcattaca tattagatca ctctttgagt ggtctttttc tttacataaa ggaggctaca
    10741 taatgagaaa ctactggtat gtatcgctaa ctaatgaata tcctcgaacc attgatgatt
    10801 gttcagtgcg tgttgtgcgt tctgtacaaa tcaaagggaa gtactctatt gtcgaaatgg
    10861 ggagagaagc agaaccatgt gagattgatg cgtgcaagct cgtttattgc ggtcatggtt
    10921 tctatgatga accaaacatt caaaataata ttaacaagaa tttgagggat tagaatgcaa
    10981 gaaatggctt aaaaatctcc aatcaggaca aatcaagtta gacaaagttt ccgatttaaa
    11041 gatattgatt gaagctgatt taatgctaaa agatattgaa aattagaaaa caaaactcaa
    11101 cctaaacaga atgtgaggtg gtgtgtattg aatggcaaga caacgtgatc caagacgtga
    11161 tgaagccaaa agaatttggt tagaatccaa cggagaaaag cagttaaagg aaattgcatc
    11221 tgaattaaat gtttcagatt ctcaggttag aaaatggaaa tcgcaagaca aatggagcgc
    11281 tgaattgatt agtaacgtta ccaatggcaa aagtaacgtt actaatcaag gtggcgctcc
    11341 tattggtaat caaaatgcta aaggtaacaa aggaaatagc cgagcttctc cgccagtggg
    11401 taataaaaac gctttgaaaa caggtgagta tgaaacaata ttttttgaca cgttaaccga
    11461 taatgagaag gacatctatt ctagtttgaa tgatgatcct tcttttgctt tgtccgaaga
    11521 aatacgactt cttaaaataa gacaacttcg aatgatgaaa agaatccaac aagctgaagc
    11581 tggactaaat gatgaagaag tcgaacgatt gcagcagcta agaaagatta aaactccgat
    11641 tgaaaaaaat ggtaaaaagc tagaaatcaa gcgtgaggtt atgcaagatg tgcagattag
    11701 cagaaaaaaa catcgcaaaa ttgatgatat tctttcaatt gaagattcat tgactcggat
    11761 tagcaaccag ttagctaaag ccatcaagca aatgaatgaa ctttatatga atgaatacag
    11821 aactgattta attaaagctc agactgataa gatccaagct gagacaaatg aaattggcgg
    11881 aaataattca ggtgaagaaa tagaagaatg gaaacaggca gttttaaatg ccgcaaacaa
    11941 acgggcggtg aaagaaaatg aataatgaat ttattccttt tgctgatatt ggttctgcca
    12001 ttgattatta ctatgataaa ccagtagctt tttgccaaga tattttgcat ttgaatcctg
    12061 atgaatggca agaaaatgtt ttaaatgatt tagctgaatt ttcaaaggtt tctgttcgtt
    12121 ctggtcaagg agttggaaaa acagcattag aagcaggagc aatactttgg ttcttaacgt
    12181 gtcgacccta cgctaaagta atagcaacag ctccgacaat gaagcaactt tacgatgtac
    12241 tttgggcaga ggtagctaaa tggttaaatg atagcttgat caaaaactta ctgaagtgga
    12301 caaagaccaa aatttatatg gttggtgatt cagagcgttg gtttgctacg gctagaacag
    12361 cgactaaacc agaaagtatg caaggttttc acgaggacca tatgttgatt gtggtagatg
    12421 aagcttctgg tgtgtctgat ccaattatgg aagctattct tggtacgcta tcaggttttg
    12481 ataataagct gttgatgtgt ggaaacccca ataatattga aggtgtcttt tatgattccc
    12541 ataattcaga ccgtgataaa tacagagttc ataaagtatc aagctatgat agtaaacgta
    12601 caaacaaaga caatatagaa atgattctta aaaaatatgg aaaagaaagt gatgttgctc
    12661 gtgtccgtat ttttggagaa tttccgaaag gtgcgttgga ttcatttatc agtcttgaaa
    12721 cagttgaatt ggctacagaa aaacaaatta gtgattcttt agtcaataaa acaaaggttg
    12781 ctcatattgg tgtcgacgta gctcgatatg gtgatgattc tacgattctc tttcctagaa
    12841 ttgctaccag ggcattggag tatgagaagt attcaaaacg tagcaccatg gaaacaacag
    12901 gatatgtcat caacatggcc aagaatctaa tgagtcaata tccgagtatt gataaagtga
    12961 tgattaaagt cgatgacact ggtgtcggag gtggtgtaac cgaccgccta gaagaactta
    13021 tagaagacaa acattatcct tttgaggtgt ttggagtgaa taacggttca acatcagaag
    13081 acgattttta cgataattta ggtactcaac tatggggaaa catcaaggaa atgttagaag
    13141 aaaatatgac agcaaatctt aacggagaac agcctgttat tgaattgcct tctgatagtt
    13201 cgttaatcaa agaattaagt actcgcaaat tcaaaatgac aagtagaagt cgtatacgtt
    13261 tagaaagtaa agatgatatg aaaaagcgaa atattggtag tcccgatatt gctgacgcac
    13321 tggctttagc gttttatgag ccaccaagtc actatcaatt tattcaattt taggaggtga
    13381 gcttttgatt agtacagtat tatctttaga gagatataag aagctacgtg ttaaatatgc
    13441 aacacaaatt gaagatggaa tgtttgatcc gaatggtttt atagaagata tgaaaccatt
    13501 ttttgctgat cgcgaaagaa aatatctagc ttatactagc gaaaagaatg aaatagataa
    13561 tagaccaaag cctaacactg atattgtaaa agttaataat aaacttcatg ctggtatgta
    13621 ttcaattgtc gtagaccaag cagtcaatca ttttactggt atacctatca aatgggatta
    13681 cgatgtatcg gaacaaaaaa gaacactcat tcaaagatta aaagataaat tcttaaaaaa
    13741 tgatatagaa cttccgacag ttccagaagc ttttaacaaa ttaacaagca atcttgattc
    13801 catgagattc gcaatgcttg attctgaaac tgcaactttt caaggagctt gtggagtagc
    13861 tttccgattg ctagagcctg tggaagaaga tgatggttgg aagttaagag caagtaatat
    13921 tgaaccttgg agagcagaaa gatatggaaa tgccggaatc tatatcaaag aaaagtatga
    13981 ctcttaccag aaaaaatttt ttcaagaaat gaaagttatg acaagaaata aaattctgac
    14041 atatgcttgc tatggtgatt tgaattttgt tacaagcgga acgttcaaaa aaattgatga
    14101 gaccgataac cctttgggga cgattacctt ggcagaattt aaaaataata cgaatcgtta
    14161 ttgtgatttt gaagtagctg aggaaattgg agatgcaatt gatcgtgctt tgtctgatca
    14221 acaaaacgaa atcgaacaat ttaagcttgc gtacatgctt gttactggca caacaatgag
    14281 caaaggcact gctaaagaaa tgatgaatca gctagggatt atcaacttaa aagcccctac
    14341 tgcaaaagct gaatatgtca caaaaaattt agcaaaagat ttcaatgaat atcatatgga
    14401 tctattgaaa aaacagtttt acaccatttg taaagcaatc gattttaacg atgaggtgtt
    14461 taaatcaaac agttctggag aagctcgcaa gtggcaaatc attagcctag aagcgaaaac
    14521 aaatactaaa gagcaatatt ttagagaagg attgaaagaa tgcgcagaaa caattgctgc
    14581 ttttcttaag tttcatgaca aagtagaaat tgagcctgaa aaaattattt ttactttctc
    14641 tagatctttg ccaactgatt taagttattt agctgaagct ttacctaaac ttgcaccata
    14701 cgtatccaaa cgaactattc agagccaaat tccatttgta acagatgttg attatgaaaa
    14761 tgaaatgatg gaattggaaa gtggaagtgc ttatccagat agtgaataca attttggcgg
    14821 aggtggcaat agtgacgata gaaacgaagt attggaccaa acgtcgagaa ctggaggatc
    14881 aagcaaggct caaacaagaa aatcagacac ttaaaaaatt aactagtgta tttcctgaag
    14941 cactgaaaga gatacaagca aaactattat cacaagctga cttacacaat atcacttatc
    15001 cagaaatgat ggagttttac agtacaagta atcagaaaaa atatcgtgaa tatgtggaaa
    15061 aaaattataa gtcattaaaa atgtatgatg caaaatacaa agagtttatc gatgaatttt
    15121 ttccaccatt tgactatgca aaagtcaatc gcttattaca aatacgatca gatgtattta
    15181 aaattcttgc agaatatgcg atggatgcag atgtgaatca atatttttct gatcgcttag
    15241 aggaaattct tcaaagaaca tattcttcta atgctaatgt ttttgttcaa cttttaaacg
    15301 ttgatatacc aaattattta ccagaaaatg aaattaaaca ttatttgaac tatccatggt
    15361 caggaaagac attttcaaga agactttggg gaaatatttc atcgcttgaa cagaaactct
    15421 ctaatgctat tgtaaaaagt gttgctagtg gagaaggcgt tatacacgca ttaaacacca
    15481 tgagattaga ctcagaaatt tgtgacatgt ttaagttaga agaatcaaag tataacaaag
    15541 cgatagaaaa tctcgttcga acggagtatg caaaatttgc acaagatggt attgaaaaat
    15601 catatttaga aacaggtatt gaggaataca acgtattgac tgcaaaagat gagagagttt
    15661 gccggatttg tggaggaaag gcaagtaaga atccctataa actgaaagat gctgtcatag
    15721 gtgaaaatcg agcacctttc catagtcgtt gtagatgtac ggatgttcct aatttaccaa
    15781 aattaggaaa ggatattgat gaagaatatg accgtttatt tggcgattta ttagatgagt
    15841 ttgcacatga ttcttttgga attaatttga aacggaggaa gtagaatgaa agattttttt
    15901 gaagcagtac taacaattaa tgtaaatgct gatattgcag aagcctacaa aacagctatt
    15961 gagtctgaga accatcctaa tggcttgaga gaccattgga atggcaatta tgcctacgtg
    16021 gttattggcg atcaaactgt taattatcaa gataatactc cagttgataa gaataccgtt
    16081 aatttaacga ttcaattatt atctcattca ttaccaaatt taaaagaaac agttgattgg
    16141 tatgaaaaga tgggatgtat tgttgttaga actgactaca aagaaggaaa gtctagtaat
    16201 taggcttttt tattttgtcc gaaatgacgt taaactagcg caatactggg cttggttgaa
    16261 tggtggggcg caactattaa aactcaaagc aatgcggggc gtgaaaacga atcgtggggc
    16321 gaaaggagaa tgagcatgaa acacaaatca ttaatgccaa tgaatttgca atactttgcc
    16381 gaaggtgacg atcaaaagtt ttcttttgat gacttcaaat cttttgtgga atccaatgaa
    16441 gaagctcaaa aatttattca gtcacaatct caaagtgttg ccgacaaaca attggaagct
    16501 tggaaacaaa ataacttaga taaaattaaa caggatacca tcaaggaata tgaggaatct
    16561 aagaaaaaca agtcacctga gcaaattcaa ctggaaaaac tacaagctga atttgaagca
    16621 gaaaaagcgt tgcgtgtgac aagtgataat aaagcatttg ttgcagaaca aattgctgga
    16681 ttagaactag atggagagtt aaaagagtct atttctcaat ttatgctaaa taatcttgtt
    16741 agttcggata cagatttcac taagaatgct gttgaaggtt ttacaagtgt tttgaatgca
    16801 attaatgaga aacatgcaga tgcattaaaa gaactacaaa tgaagtctgc atttggtgga
    16861 actcaacaat cgaataacca ggttcagcag aacaatgaaa cattaacaaa tccagaagaa
    16921 caattaggac aaattcttca acaatttaac taggagagtg aaaaattatg aaaaaaacat
    16981 ctttaaataa tttagagtat ttggatattt caccagcgat taatgctatg caagtaccaa
    17041 atacaccttt tttaagctat ttacttggtg ctggaaaaac agagccagca aactcgacag
    17101 aaattaaatg gcgtgaatat gatatcaaca acgatgattc ttctgaaaaa cttgagggcg
    17161 gagaatatcc agatgctgaa tcaggtcgaa cttggtttaa caactatact gaaattttta
    17221 gaaaatcaac ctctgtatct ggtacattag atgctattaa tgtgaatgga gtcggaaatg
    17281 aattaactaa tcaagtagca ctacgtggta tggaaatgaa aattgatttg aaccgaaaat
    17341 tgattactgg tgtaaaagct gatgaaaatg gttctaaagg tcgtcgaatg aatgggattt
    17401 tgaacttgat caattcagca aataaggcag aaacagccac tgcgggtgca gtaacaagaa
    17461 aagatatcga tactttattt aaattgatgt atgaaaaagg ttatatggga gaaaaactat
    17521 gcttgatttc tccagatatg caggagttaa tgactgatga gttagatgga aaatcaacaa
    17581 aaattgttca gttcggggaa agagtaactt ttggattgca aattggaaat atcgtgtcta
    17641 attacggtac aggtattgct ctactagaac catcattgcc aaaaggaaca attgccgcaa
    17701 tcgatactaa ttatgtgaaa ctacgtccat tacgtgaatg gagagcagaa gaacttgcaa
    17761 aaacaacgga ttctagacgt atcggtcttg taggtgaata ctctcttgaa tacaatgctt
    17821 caaactctgg ggcaatttta aatttaaagt ctgaataaaa gggagttagt actccctttt
    17881 ttgataggag gaattacagt ggtaaaaaaa gatgaaacta aaaaagatga agtcgtgaaa
    17941 tatagagtag gtaaaactaa aaattttgtt gggtttgttc atcctaaaac tcgtagattt
    18001 atcacagcag attcaaataa cgaatttatc atttctatag atgataaaga agcaattgca
    18061 attttggaag atgcaattga tgttaatgaa atttaggaag tgatctgatg gatgaatcgc
    18121 taaaaacgga aatcattgag tctacaaaag aagattttcc agatttgagt gaagaacgca
    18181 taactaattt attagaaata attttgctag aaattgaatc atacaacact tgtaaaaatg
    18241 atatttcatg ggaaaagtta aaaagcgtga ttagtgaagt gttgtataaa ataataaaaa
    18301 atgaatcaga aaaaacagta tcttcagtta gacgtggcga tacaacgatt agttatgctt
    18361 caacaacaaa tgatgttagt gagttacttc taggctatgg tgatttgata cgaagagtta
    18421 ttggttgtgg aggattggag tttttttaat gaatgaagca gatattttag aaactactta
    18481 tgaagatagt tgtattattg aaagactgac ggacattgaa gattctaata caaatattac
    18541 tattcaagat tacaaaaaag tatatgataa tcctatttct tgcgctcttt cacaaggtca
    18601 aatcgatgga ctagcagtca tagaagatgg agagatggta aatgtttcaa ctgacacata
    18661 taaattattt gttcatccta agattaaact aaaaaaagga gatcgaataa caataactca
    18721 aaaggccagt ggcttaattt tttctctatt tgctactaag cctttttact atcctagtca
    18781 ttgtgaagtg aatttgatag gaagtgagaa aaatgggtaa tctcaaatat gaatcaaatg
    18841 cagaaaagat cattgagaat tttaaaaata tgactgtaat tgctcaaaaa gaaggaatat
    18901 ccttcgttaa cgattcaatg aataaagtcg ttagtttagc taaaccatta actcctgtaa
    18961 aatcaggtaa tttaaggcga ggatatcgtg tagtaaaagc tagaaaacta tcaagcggtc
    19021 gaattgttgg agaagttata aacaatgaac attattttaa atatgtagaa gaaggtcata
    19081 ggactaaaaa tggtggcttt gtcaaaggga aattcatgtt aactcgtgca acgaatcttg
    19141 caaatatgtc ttatattcct cgaagattta aacaaatggc aataaaaatc gttaagaaag
    19201 gaaagtaaca tgaaagatga aatcattgct gcaatcagta gcaaattaaa agaaatctat
    19261 cctgatggga caatatatct tgattcagtt atgcagtcaa ctaaagattt ttatttcgtt
    19321 ttatcagtaa tggaatctgg aactgaaaat gtaggaattg atattcaaaa tgtttctttc
    19381 ttaattgata ttgcattgat tgataataac cctaatagaa atttaattaa tgaattagtc
    19441 tcacgttgtg ggactttttt taatacgatt acaatcgatg agcaaatatt atttccaaaa
    19501 gcctatttac ctgatgaagc agatggtgtt caacatattc gttttacatt agaatttcca
    19561 caatatattg aatggagtga aagataaatg ggagaaaaaa gaagtaaaac cggaattatt
    19621 tctgtcgaaa aacctacttg gtttcctttg gaagatgaga cagggaattt tccggtatat
    19681 ggtactgcgt tgccaatggg aacagcagta agtatcaaac caacagctaa ttatgaaaca
    19741 acacaagatt atggggattc agtggttcaa gatcaattta cagcttttgg tggtgcagaa
    19801 gttgaactgg aagcaaatgg atatactcat aaagttttat ctacaattac tggagggaaa
    19861 attgttaaag gtggtgcctt gcgttctgga gaagatattg cgcaagatgg tgcgtttgca
    19921 tatagacgta aaaaatcgaa tgggaaatac cgttatacgg tcttttataa aggacagttt
    19981 gctctagatt cagatgaaac atcaacgatt gaaggaagta aagtaagttt tactcatcca
    20041 acttggaagg gttcatttgt agatgtacca ggacttggat atatgtattc agttgatgaa
    20101 gatgatgaag gcgtggataa agcaatgatc ggaaattggt ttacaaaagt agcaattcca
    20161 attgaagaag cagaactttc aggaggtaca gaataatgtc aaaatatcaa acaacgatta
    20221 aattaacgaa aaaagacgaa gaaggtaaat atgagcaagt acaatttaaa tctgctgaat
    20281 ttttaccagg aacagttgta gaagatgctg caggagttat ggaagaaatg caaacagcga
    20341 ctgataagca atcagttaaa aaagctttaa gtcgtgctta ttcatttatt gcagatactt
    20401 tatttgaggg acaatttact ggtgaagatt attgcaaagg aattgatgct cgtgagattg
    20461 cctcattgac aggaaaatta ttgaagtctg ttactgcagg ttttgatgaa acttatacag
    20521 agacgaaaaa aaagtaagtg aggcgctcaa gtcaccttca tttaagtatt cgattactta
    20581 ccgagaatta gatataaaga cgcaattact cgaagcaggt tggacgttac cagaaattga
    20641 acgtactgac ttggatgagt taatgcgtct ttttgctttt agagatgcgg taaaagagca
    20701 tgaagatgtt gagtactatg ataatttcac tcaattttag gaggtgatac tttgaataac
    20761 gatgacctaa ttctgaagat gatactagat gaatctggtt ttactgccgg tatgaataat
    20821 gctgtcaaaa agttgaactc ttttgatgaa acaatcgaaa gaaaaagtag aaatagtggc
    20881 agttcattag gtagtatttg gaaaatattt gctggaagtt ttctagctag cggagtaact
    20941 agaatcgtag gagctggctt tgatctaatc aagggttcca taagtggagc ggttgatcga
    21001 gtagatacga tgaataacgc tctacgaaat ttccaaaata tgggatttag taattcagaa
    21061 attatgaaga atatcggaaa gaatgggctt ttatctcaag gtattcaagg acttcctact
    21121 gctttgaatg atgcgataag tcatgttcaa cttcttgctt cttctacagg tgatttaact
    21181 cgttcaactc aaatatttaa agctttaaat gatggaattc ttggtttcgg tggttcaact
    21241 gatcaagtta atgaagctgt tattcaatta tctcaaagtt tctcaaacgg aaaagtagac
    21301 gcacaaactt ggaactcaat gatcaacgct caacttggac ctactctttc tgctattgct
    21361 aaaaagatgg gaattacaat gggagatctg aaagaaggtt tatctcaagg taagatttct
    21421 gttgaagaat tccaaaatca attaatagaa atggatacca aaggtggcgg aggacttaaa
    21481 tcattaagtc aaatcgctaa agattctact aagggaatta aaacctctat acaaaatgca
    21541 aaaacagctg ttacacgtgg tgttggtgag gtaatagaag gattaaataa agcattagta
    21601 gattcagatt taggtggatt taagggaatt attgataaag tgggcagttc aatggaatcg
    21661 ttcttaaaag taattgctgc aaatattcct acagcagtat catttttaag taatttattt
    21721 aacgaaattc aaaaatttgg ttctgctttg aaattcatga tgccctttct tgttcctgca
    21781 gctaccgctt ttggagcgtt catgtttcaa cttaaaggta taccagcaat aataaaaagc
    21841 ttcaataatt ttaagaatgc cataattggc gttggaaatt cattaaagat tatgggggca
    21901 atagctgctg ctaatccatt tgttttaatt attggtgcag tcgttggtgc aatagctgtt
    21961 ttcggttact ttatggcaac caatgaagag cttagaaaca aggttatatc tatttggaat
    22021 gatgtgaaag attccgtaat tggtgtatta aaaaatataa aggactgggg aattgatact
    22081 tggaattctg ctaaagaaat ggcatcaaat gcagttgagg gtgtcaaaga tgcttggtca
    22141 ggaacaaagg aatggttttc taacacctgg aaaggtatta aggatggggc cacaggttta
    22201 tttgataaaa cagtagagac ttcaagaaat gcagtcgata gtgtaaaaaa tgcatggtct
    22261 aatatgaaga aatggttttc tgatacttgg caaagtataa aagattcagc cagagaaaaa
    22321 tggaatggaa tcaaaggttc tattatgtca gttgctggtc cattgattac aggtataaag
    22381 aatgcatttt tacatgttac tttttatcta gagaccttat ggaataattt agttgagatt
    22441 ggtaaaaacg tctttgagat tttaaaaaat gttatacttg cgccggtttt gtttattacc
    22501 tccatgattt ctggtggatg ggaagaaaca aaaaataata tgattgctgt ttggaataat
    22561 attaaagaaa gtgccataaa tatttgggaa tctataaaga atatatttgt aagttatttt
    22621 acaaatat
//
LOCUS       DAZKCC010000045        22336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000045 DAZKCC010000000
VERSION     DAZKCC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.045"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG8VH_001743"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG8VH_001743"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9110299.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG8VH_001744"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG8VH_001744"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9110300.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG8VH_001745"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG8VH_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9110301.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG8VH_001746"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG8VH_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9110302.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG8VH_001747"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG8VH_001747"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9110303.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG8VH_001748"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG8VH_001748"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9110304.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACG8VH_001749"
     CDS             complement(8557..9027)
                     /locus_tag="ACG8VH_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP9110305.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACG8VH_001750"
     CDS             complement(9073..9498)
                     /locus_tag="ACG8VH_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP9110306.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACG8VH_001751"
     CDS             complement(9565..9834)
                     /locus_tag="ACG8VH_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP9110307.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACG8VH_001752"
     CDS             complement(9845..11440)
                     /locus_tag="ACG8VH_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9110308.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG8VH_001753"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG8VH_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9110309.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG8VH_001754"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG8VH_001754"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9110310.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACG8VH_001755"
     CDS             15906..16880
                     /locus_tag="ACG8VH_001755"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9110311.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACG8VH_001756"
     CDS             17050..18450
                     /locus_tag="ACG8VH_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP9110312.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACG8VH_001757"
     CDS             complement(18528..19250)
                     /locus_tag="ACG8VH_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9110313.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACG8VH_001758"
     CDS             complement(19329..19835)
                     /locus_tag="ACG8VH_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9110314.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACG8VH_001759"
     CDS             19958..21757
                     /locus_tag="ACG8VH_001759"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP9110315.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..>22336)
                     /locus_tag="ACG8VH_001760"
     CDS             complement(21825..>22336)
                     /locus_tag="ACG8VH_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9110316.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6176 a         4872 c         4033 g         7255 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttg
//
LOCUS       DAZKCC010000046        22174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000046 DAZKCC010000000
VERSION     DAZKCC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.046"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            244..2079
                     /gene="tdc"
                     /locus_tag="ACG8VH_001761"
     CDS             244..2079
                     /gene="tdc"
                     /locus_tag="ACG8VH_001761"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9110317.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2384..3862
                     /locus_tag="ACG8VH_001762"
     CDS             2384..3862
                     /locus_tag="ACG8VH_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9110318.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3828..5159
                     /locus_tag="ACG8VH_001763"
     CDS             3828..5159
                     /locus_tag="ACG8VH_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9110319.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5273..7825
                     /locus_tag="ACG8VH_001764"
     CDS             5273..7825
                     /locus_tag="ACG8VH_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFP9110320.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7832..9271)
                     /locus_tag="ACG8VH_001765"
     CDS             complement(7832..9271)
                     /locus_tag="ACG8VH_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110321.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9246..9428
                     /locus_tag="ACG8VH_001766"
     CDS             9246..9428
                     /locus_tag="ACG8VH_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110322.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9617..9979
                     /locus_tag="ACG8VH_001767"
     CDS             9617..9979
                     /locus_tag="ACG8VH_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9110323.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10166..11023)
                     /locus_tag="ACG8VH_001768"
     CDS             complement(10166..11023)
                     /locus_tag="ACG8VH_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9110324.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11143..12054
                     /locus_tag="ACG8VH_001769"
     CDS             11143..12054
                     /locus_tag="ACG8VH_001769"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9110325.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12061..13707
                     /gene="mdcA"
                     /locus_tag="ACG8VH_001770"
     CDS             12061..13707
                     /gene="mdcA"
                     /locus_tag="ACG8VH_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP9110326.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13809..14108
                     /locus_tag="ACG8VH_001771"
     CDS             13809..14108
                     /locus_tag="ACG8VH_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP9110327.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14098..15774
                     /gene="mdcD"
                     /locus_tag="ACG8VH_001772"
     CDS             14098..15774
                     /gene="mdcD"
                     /locus_tag="ACG8VH_001772"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP9110328.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15778..16371
                     /locus_tag="ACG8VH_001773"
     CDS             15778..16371
                     /locus_tag="ACG8VH_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP9110329.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16395..17471
                     /locus_tag="ACG8VH_001774"
     CDS             16395..17471
                     /locus_tag="ACG8VH_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9110330.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17496..18365
                     /gene="mdcB"
                     /locus_tag="ACG8VH_001775"
     CDS             17496..18365
                     /gene="mdcB"
                     /locus_tag="ACG8VH_001775"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFP9110331.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18476..19240
                     /locus_tag="ACG8VH_001776"
     CDS             18476..19240
                     /locus_tag="ACG8VH_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFP9110332.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19729..21822
                     /locus_tag="ACG8VH_001777"
     CDS             19729..21822
                     /locus_tag="ACG8VH_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9110333.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21847..22149
                     /locus_tag="ACG8VH_001778"
     CDS             21847..22149
                     /locus_tag="ACG8VH_001778"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9110334.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7178 a         3787 c         4724 g         6485 t
ORIGIN      
        1 tatttgtttg ttcgcttttt ttaggttatc tattttagaa gactgattta ttgaaaaatt
       61 gttcgctttt ttgcgttgaa ttatcagaaa aatccgactt tatttatttt tgtttgtaaa
      121 aatctacgtg gatttgatgt tataaaaaaa aaattctcat ttttggcgct atagtgaaga
      181 ggtgttacag ataaacacat gtatctgcaa gacattgacc aacaaaatat ggagggaaga
      241 aaaatgaaag atatggatat caaggccgtc tttataggcg acaaagctga aaatggacca
      301 gtttataaaa tgttgttgaa caagatggta gatgaacatc ttggatggag agaaaattat
      361 attccttcag atatgcctgc aattagcgaa ggagataagc tcactccaga ttatctagcc
      421 acccgcgacc atatgataga ggtcttggat gaagtcagtc agcgattacg cgcaggatcg
      481 atcccatggc attcagcagg ccgttactgg ggacaaatga atgctgaaac attgatgcct
      541 gctttattag cttacaacta tgcgatgctg tggaacccaa ataatgttgc attggaatct
      601 tctatggcaa cttcgcaaat ggaagcagaa gtaggacagg attttgcttc attattcaat
      661 atgacagacg gatggggaca cattgcagca gatggttcta ttgctaatct tgaaggatta
      721 tggtacgccc gttgcatcaa atcgattcct ttagctgtca aggaagtgct gcctgaaaaa
      781 gtgaaaaaaa tgtctgagtg ggaacttttg aatttatctg ttgaagaaat tttggaaatg
      841 accgaaagct ttacggacga agaaatggat gaggtcaaag cagcttcttc ccgtagcgga
      901 aaaaatatcc aaagattagg gaaatggctg gtccctcaaa caaaacacta ttcttggatg
      961 aaggcgttag atatttgcgg agtcggctta gatcagatgg ttgctatccc agttcaagag
     1021 gattatcgaa tggatattaa tgctttggaa aaaacaattc gtgaattagc tggacaaaaa
     1081 atcccaattc taggtgtggt tgctgtcgta ggaacgacag aagaaggtca agtagacagt
     1141 gtagataaaa tcgtccaatt aagagaaagg ttaaaagatg aagggatcta tttctattta
     1201 catgttgatg ccgcatatgg cggctatgcg cgttcactgt ttctaaacga agcaggagaa
     1261 tttgtgccgt atgcttcctt agctgaattc tttgaagaac atcatgtttt ccaccactgc
     1321 gtaacgattg acaaagaggt atatgaaggg ttcagagcta tttcagaagc ggattctgtc
     1381 acgatagatc ctcataagat gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa
     1441 cataaaaata tgagaaatat catttcttat ttcgcgccat atgtgtttga aaaatcagta
     1501 aaggctccag atatgttagg agcttatatt ttggaaggat caaaagctgg agcaaccgcc
     1561 gccgcagtct ggacagcaca tcgtgtattg ccattgaatg tgaccggata tggtcaattg
     1621 attggggctt caatcgaggc agctcaaaga ttcagagaat ttctcgatca tctgactttt
     1681 actgttaaag gaaaaacaat cgaagtctat ccattgaatc atcctgattt taatatggtc
     1741 aattgggtat tcaaagaaca aggctgtaca gatttgaacg ctatcaatga attaaatgaa
     1801 aaaatgttcg atcggtcttc ttatatggat ggcgatgttt atggtgaacg gtttattact
     1861 tctcatacta catttacaca agaagattac ggtgattctc cgattcgttt tgttgaaaga
     1921 atgggtctaa cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct acgtgcggct
     1981 atcatgactc cttatttgaa tgatgatcga attttcaatt tttatacaaa aaaaatcgcc
     2041 aaagcaatgg aaaagaaact aaacgaaatc atccaataga actgattttt gtgtgaaaga
     2101 ataacaagag acatatggag aacagacaat tatttgcttc atcaaaacaa ttgtatttcc
     2161 ccctgttttt catatattcc tcgccaagat attctttttt ataaaacgtt ctataagttg
     2221 gatcgttcgt ttcatgtatc aaacccagtt cactaagatc gttcaatgga tgtttcggtg
     2281 atactgggtt ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga acaaaaataa
     2341 aagaaaacaa aaacatatgt cgctattgaa agaaggaata aagatgaaag atactggttc
     2401 atcaaagcaa atcacgacca tgacctttat cggaatgacc tgcgcccttg tagcaagtgt
     2461 acgaaatatt ccagatgtag cagcaacgag ctggacgatg attttttaca tgctggttgc
     2521 agtattattg tacgcttttc caatctcatt gatctctggt gaatttgctg gaatgtttcc
     2581 ccaaaaaggt ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt
     2641 tgtctcatgg ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg cttcagcact
     2701 cgctccgttg tttggtaata ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac
     2761 attagttgtc attttagtcg tttattggat catcactttt ttgaatttga aatttgatat
     2821 ggcaaaaatc ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt tgacgatgat
     2881 gctcttatta gggtttgctg cgtggatcaa agttgggatc gtaccaaccg gaacattagg
     2941 cagtttttca tgggacaaat taataccaga tactacgact gcatcctcat ttgtttattt
     3001 tgcgccgatc atgtttattt ttactggtat cgaaatgtcc tctgtgtata tcactcgatt
     3061 ggaaaatcca gttaaaacgt atattcgcgg agtatttgcc gcgttaattt ttatattttt
     3121 cgtcaatatt ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt
     3181 gaataatatc gcgcaatcta tttcgattta ttgccaaatt cttggtttgc cacatcttat
     3241 tgtgaatctg tttagtttac ttgtcttcat tggtgtagca gtccaattat ctgcttgggc
     3301 ctcaggacca gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt
     3361 caatttttgg aagacgaatc aatttgacgt atccaaatcc gttattttga ctcagtctgt
     3421 cattatctca atctttgcac ttttttattt gctgattcct ggcgtcaatc aagcattttt
     3481 gatgctcgta aattcaacta ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc
     3541 agttttgaga ttgcgttata cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa
     3601 agagaaaaag agcaatcttg gagtttggac tgtcgttatt gttttatttg cggcaatcgg
     3661 attttctgtc ggactgacga tgaaagctgg aacatggatc aatctaattg cagtcacagc
     3721 catcagtgtc attttgtttg tagtaccact ctgtattgaa aaaataaaga aaccttcttg
     3781 ggaacaggaa gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg
     3841 caatgaaacg gactctaact aatcgaaata tccagttaat cgcattaggt ggcgcaattg
     3901 gaacaggatt atttttaggt tcagcaaagg caattgcatc agcagggcca gctgttattc
     3961 tcgtttattt gatatgcggt ttggctattt atggtttgat gcgggccatg ggtgatttat
     4021 ttttagcaaa tagtacattt aactctatca gtgatttcgt tgactattat ttaggcaaaa
     4081 agtgggcatt ttttgtcaat tggacttatt ggctttgctg gcttggagct ggacttgcgg
     4141 aattaacagc aataggattg tatatccatt tctggtttgt acatgtacca gctttgataa
     4201 gcggaggtag cgcactaatg atcttaatgg cagtcaacct tgttgcagtc aagtggtttg
     4261 gtgaattgga atcttttttc tcaatcatca agatcttggg gattttcctt tttatcttat
     4321 tgggtatagg attgagtgtc tcagattttc atacccagtc tatcggaatc aaagaaaatt
     4381 ttggacagat cgatgaatta ctaccgtttg gctttgctgg aatggtagtc tcatttccta
     4441 tggttttatt cagttttgct ggaattgaaa tgatcggctt aaccgttggc gagacaagtg
     4501 atccgcaaaa gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt ctctttttct
     4561 atataggaac gattttgtcg attttatttg ttgttccatg gcattgttta gatttaaaag
     4621 aaagtccttt agtgactatg ctgcaagtga tccattgcca aggcggagca gcaattgtca
     4681 atctaatgat tttggttgct tctttgtcct cagcaaatag tgcagtctac agtactagca
     4741 ggattttata tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta
     4801 agaaaggcgt acctttacac ggtattttaa ttacaggaat gttatttttt gccggtcttt
     4861 gtctacattt ttttgtagct gatagtcgat tgttttttca gcttttggca ggcatgacaa
     4921 cgagttgttt tctatttgtg tggtcttcga tcttgtgtgc acatgttcaa tttattaaag
     4981 aaaagaaacg gaacactaca caaagatata aagatcttgc tttgttggtg ttttttactt
     5041 caattgcttt cagcttgtta ttcgaaagca cgacacgctt tattcctttt ttattggtta
     5101 tctggtttgt attcttaaat tttgtatata aaagaataag caatagaaat atcagttagg
     5161 cccagataca ttttgcaaaa cgaattattt cagacccccc tgaaataatc gatacgcctg
     5221 aaaaaacttc cctgcgtata ataacagtat caattaaaca ggaggggata ggatgaactg
     5281 gtacagattg acggtagaag atgtattgaa aaactatcaa acatcaacag aaggcttgtc
     5341 gcaagaagaa cgacagaaac gcctgactga tcaaggatat aatgaaataa aagcaaggca
     5401 gcagacaaaa cgatggaaaa aaatagcaaa gcattttact gatctgctga tgatcgtgtt
     5461 gatcattgcc tcttttttga aatttgcttc tagtgaatat attgaaggaa gtattatttt
     5521 attcgtagtg atcgtcaatg gactcgttag ttactggcaa gaaagaaaag cggaagaatc
     5581 attgaatggt ttgaagcagc tgatgggaca agaagcgata gtactatcaa acgggatgca
     5641 agaaaaaatc cctgcgaaag aactggtaaa aggagatgtc attgtcttac gtgccggaga
     5701 tgtcgttcca gcagatatcc gattaatcga agcacatgat ttattagtgg aagaatcgat
     5761 tttgacggga gaatcagaag catctgaaaa aagtcatgcc gttttgacag aagaagaatc
     5821 gatcggcgac cagaaaaata tgggtttttc aggaacgctt gtacaatctg gttctgcagt
     5881 cggtgtcgtt gtagaaacag gaaacacgac agaaatcggt aaaatcaatc aagcattgca
     5941 atcggtcgaa caacaaacga cgcctctgat taaaaaaatc aaccgattaa acaaacaaat
     6001 ttttcaaggc attctttgtt tgagcctatt cttagttatt tttacgacat ttcgatatgg
     6061 tatggattgg catatccttc tttcggctac gattgctttg gtggtgtcaa tggtacccga
     6121 aggattgcca gctgttttga ccatgattct atccgttgga gtacatgaaa tggcaaaaga
     6181 aaaggcgatc atcaaaggac ttccttcggt agaaacatta ggttctatga cagtgatctg
     6241 ttcagataag acaggaacat tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa
     6301 agaagaagcc tgcgtgttgt ccattatgaa aaactgtcag gaattaaaaa caaaagaaga
     6361 acagaaaaca gaaaatttaa gtggcaatcc gacagaagtt gcgctgattc gacacacaga
     6421 aacagccaat ctacctttga aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa
     6481 atatatggca acgatgcatg cggaggaaaa aggagcggtg atttacgtca aaggtgcacc
     6541 agaagtatta tttgctaaat caactctttc ttctgcagaa caagaagcat ggagtcagac
     6601 agctgcagaa tttgcgagaa aaggtcagcg tgttttagga tttgcttata aaaaagtaga
     6661 ttcgaaacag gaactcacgc atgagacttt aacccaatta acttttgcag ggatcgccgg
     6721 attgatcgat ccgccaaaag aaagtgcagt caaagctgta aaggaatgcc aacaagcagg
     6781 gatctcagtg aaaatgatta caggggatca caaagacaca gcaaaagcga ttgcggaaca
     6841 aattggctta aaacatactg ccaaagtttt agaagggatc gatttggatc ttatgtctga
     6901 tgaagagttg attcagcaag tgccaatagt cgatgtcttt gctcgaacga caccagaaca
     6961 taaactgcga attgttaaag ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga
     7021 cggcgtaaat gatgcacctg ctttgaaaag atccgatgta ggaattgcta tgggaatcaa
     7081 aggtagtgaa gtaagcaaac aggcagctga tatggtttta ggagatgata atttccatac
     7141 gattgcaaaa gccgtaaaag agggccggcg gattttggat aatttgcaaa aaaccatcaa
     7201 tttcttcttg ccaactgcat tagctcaagg attgatcttg atttgggcac ttatgctgaa
     7261 tcgcccgcta cctttgtccc cagtacagat tttatgggtc aatatggtaa cgacaatcac
     7321 tttatcttat gcactagggt tcgagtcgcc agatccagaa atcatgaagc gcccaccaag
     7381 agatccaaag caaggaatcc tatctggtta tcatttgttt cggatcatct atgtctccct
     7441 actgatcatg gttcctgcct atttattggc tatgcagttt gaagggcaaa gcttacagca
     7501 aaccattctt ctgcaaaata tcgtcttagc tcaagctgtt tacatgatca attgcagaga
     7561 gttgtccaag ccgtcattga accaaggtct tttgcaaaac aaagcgcttt ttctttcatt
     7621 aggtatcctt gctttattgc aagggatggt tatttttcta ccagtcggcc agcagctgat
     7681 cggtacagta agtttaacga tgacacaaca gttattgatt ggagcaaatg tgattcttct
     7741 attcttagtt gtagaaatag aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa
     7801 aacagctaga gaatacagtc gctagccgtt actataaaaa agttttgaaa gtgagttcag
     7861 tcaaacgatt gagctcattt ttcgttggaa tagaagaaat gtatacaata gggatatcta
     7921 gagaatctag agggaaattg gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa
     7981 gatcggttag ttcgataggt aagaagcaga cacgttttcc taagtaatct tttaattcct
     8041 gctgcaagaa cgccttccat gaaggttctc cttggaaaat aaaatagaca ttgaacaaat
     8101 tcggtttgat agaaaaaatc gtttgttgga tcagtagaga caagagttgg agaaagtaat
     8161 ttggttcatt gatttctatt tcttttttta gatcgttcag taaattctct ttctgttgat
     8221 acaagtgtgc atattgctga tcatatgttt ctaaaaaacg atggtattga cggtccaatg
     8281 cttcaatcaa tatcggcgat tcatgatatt ttaatatgag gagagccaag ttatcagtca
     8341 gagatgtttt ttttagttga tcaagagaca gctctgaagc tagttcctct acaaacgttt
     8401 cgaggacctc taaaaatgga gaatgatgca ggcagcgagc ggtttcttgt ccatagttat
     8461 tgttgtaatt ccaactttcc agaaagcaga aagaagcaaa agaaagctca gtgtctttaa
     8521 gataaatacc ttccgttaaa tacaattttt tcaatgcaga gtcatacagg gaggagagtg
     8581 tgctgttggt atggatagaa gaatctaatc ggcaattcgc ttttatacgg atcgtattga
     8641 taaagaacca acatgttccg aaaatctctt ctgtttcaac cgctagttca gtttgactga
     8701 ggtttcttaa aaattgaaaa taatgggatt catggatcga ataatcctcg aaaaaatagt
     8761 tgttatgagt gaaaggaatc agcaatcgat gataaaaagt acgaatcgca cgctcttttc
     8821 caagaataca agctgtatat ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct
     8881 gattgatttt agtcacatgt cttttcaatg tcccataaga aagcccatgg tcggctagaa
     8941 aatcagggac agcaactttt tttgtaaaaa gcagattttt cagcagttcg acactgactg
     9001 attgtttgaa aaaaatctcc caaaggtcat ttgttaataa attttgctgg ttttgcaaac
     9061 tgattcctgc ttgatcgctg atgatgtccc agccttcggg cagctgagag cgaatagatt
     9121 gaaggtcaga aaaaatcgtt cgttcggttg tccctatgat ttcagctaat tcatttacgg
     9181 ttatctttgt atgattcagc aattgttcta ctaaagaaac atgacgtcga atatcttttt
     9241 cggtaatgat ttttttcatt agtgaataca tcaatcagac tcccttctat tctaagaata
     9301 cggagaaaat aaacggtggt caagtttcaa ctgatttttc aatcattgtt tatttttctg
     9361 gagcatttac tgatgataag aatttcatca tgaacttgaa aaatgatacc actaaaatca
     9421 aaaaataggt gggcaaacaa taataaacat tttaatagta gatggtagca agaccaacac
     9481 aggcggattg aacatttaag agttgataaa aaagaaaaat atcgaagtat ttgattagca
     9541 actacttgta ttcatttttc tattcttacg tggtatcttt gaataaagcg aatcaataag
     9601 aaagagatgg aagcagatgc taacgaaaaa agaactaatc gatcacatca atgaaacata
     9661 caatttgtct ccagaatata tttttaaaaa atttcctgat tattgcgtat tcagacatca
     9721 aaaaagcaaa aaatggtttg gggtcctgat gtctgttcca gaagaaaaaa tctatgatga
     9781 tgcaagtgac cataggatca tcaatattct tgatttgaag gtcgatcctg aacttggcgg
     9841 aattttgcga ggaaaagagg ggtattttcc agcctatcac atgaataaag aacattggat
     9901 ctctattgat ctacgtaaaa tagaaaagct agatgaacta gcagatttga ttgatggaag
     9961 ttttaaatca acgcaatgaa tgtctaggac aatagagaag aggttgtgac agaaatgttt
    10021 agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg ggaaatttcg
    10081 gcttattttc taagaaatta cttctggaac accgtttgtt cgaaaaaaga gctgcgacaa
    10141 gaattttgtc ccagctcttt tttttttatt ttcctaaata aaattcccgc atcgtttttg
    10201 caatcatttt gatcaaagga tgatcttctg tgaagctaga agataaataa attgttcgtg
    10261 tcaagggcaa atctttgatt tcccgcaaga cgaaaggatc atttttatcg taagaccaag
    10321 aaacttttgg gaaaaagcta acagctaatc cttgcttgac aatagaacgt aatgtcgcag
    10381 gatcatctgt cgtcgaactg atcgtcaatt cgatactttt cgcttcaagt gctgcatcca
    10441 gtgtatgacg gaaggcgttg ctctttgaca gcatcaagat gggggtgcgt tggatatctt
    10501 ctaattgcag atgttctttt ttagcaagag gatgtttctt aggaatggcg acataaacag
    10561 gttctttcat gataggaata ttggtcatgc caggcttcat ctccgaagtg atgtacagta
    10621 caggatcggt aatatcatct ggtaaatgat gttgcatgat cgttaattgg atatcaggat
    10681 gcttttccat aaaaattcgg accaagttcg gaatagaaac agaggctacc tctaaataca
    10741 atatgacgga agtaacgatc ttctcattga ccgaagacaa taatttgatt ccttcttcta
    10801 atgtgtctaa tgtcttgact gcatatggat aaaattcttc tgcataaacg gtcgtctcaa
    10861 tgtttctgcc gctatggtaa aacaaaggga cacctaattc gttttccaaa tgtttgatcg
    10921 cacggctcaa ggcaggttga gaaataaata attctttggc tgcttgggta acattttttg
    10981 tttcgaatac ttttttaaaa tactgcagat gaagcaattt cataattatc catcccatcc
    11041 ataccttttt gttatggttt tttctttttt tggcattttt tattatagca gaaattgact
    11101 aagatagcag tggaaaaagt aagtgctgag gaggcgaggc tcatgagtac aggtttctta
    11161 tttcctggac aaggcagtca gaagttaggg atgttagcgg atatccctga aatgtacatg
    11221 gaacgtttag aaaatgtgac ggggtatcgg ttagttgata aagaagaaaa ctatcaagat
    11281 acggttttta tccagctagc attactgaca aaagcagtat tttatttaga tgaaatggaa
    11341 aaaaacggta ttcagcctga tattgcagca ggacattcga ttggtgcttt ttgtgcagct
    11401 gttgcttgtc gatctttgag atttgagaat gcagcggaac tagtctatca ccgcgcagtc
    11461 ttaatgaaag aagcttatcc aagcggttat gcaatgggcg tagtagtagg tgtgacgaga
    11521 agcgaagctg aggaaattgt agcaaaaaca tttgatcctg accatccagt ttatctgtca
    11581 aatgaaaatt gtccgatgca acatacgtta tctgggagta tcgaaggttt atccgttacg
    11641 ttggcagaag ccaaacggca ccaagcgcaa acagcgaagc tgctgaaagt ccctgtgcca
    11701 tctcactgtc tcttgatgaa tgatacggtc aagaaattca agccgtatat gcagtctgtc
    11761 catctatctg atgctagatg tttgtatata aaaaatacgg atggaagagc cactcaggac
    11821 gcagaagaga ttcgtaaaga tttgttggaa aacctagctt tccctgtgca gtggaaccaa
    11881 atgatggact tggcaaaaga attaggaatg gatctgacga ttgaatttcc ccccggcaat
    11941 actttgacaa agctgattca tacaaaattt ggtgtagata aagggatccg tacgatcaat
    12001 ctagaccaac atggtattga tgacgccacg tttctatatc aaaaatggag atgactaatt
    12061 atggaaaaac caaaccgcaa ctgggcttct aaacgcgaaa aaaaagcgca gcgattggaa
    12121 aaaatcagca acttgctcaa tggcaagtat gtagaaactg aaaaaatcgt agaagtaatg
    12181 gaaaaactga ttgctcccgg cgacaaagtt gtattagaag gcgacaatca aaaacaagca
    12241 agttttcttt cagaaagtct tgaaaaagta gatgcagaaa aagtgaatga tctgcatttg
    12301 atcatgtcta gtatctcacg accagaacat ttgaatattt ttgaaaaagg aatcgcaagc
    12361 aagatcgatt tctcttatgc tggtgcacaa agcttgcgtg tcgcccaaat gattgaagac
    12421 ggcacaatga aattaggtga tatccatact tatttggaac tttatggacg cttattcatc
    12481 gatttgattc caaatgttgt tttagtagct gcagataaag cagataaaga cggcaacttg
    12541 tacacaggtt tcaatacgga agaaacacca acaatcatcg aagctgcagc tttcaaagat
    12601 ggtatcgtga tcgttcaagt taacgaagtt gtcgattctt taccacgtgt agatatccct
    12661 ggtgactggg tagacgttgt cgtgaaagca gataaacctt atcaattaga agctttattc
    12721 acacgagatc ctcaaaacat cacagaattg caaattctaa tggcaatgat ggcaatcaaa
    12781 ggaatctacg cagaacacgg cgtacaatct ttgaatcacg gtattggatt caatacagca
    12841 gctatcgaat tgttactgcc aacttatggt gaatcactag ggttaaaagg gaaaatcgcg
    12901 aagaactggg tattgaaccc acatccaacg atgattccag ccatcgaatc aggttgggtt
    12961 gaaagcatcc atagttttgg tggcgaagtt ggtatggaaa aatatattgc tgctcgccca
    13021 gatgtctttt ttgtcggtaa agacggcacg atgcgcagta accgaacatt atcacaagct
    13081 gcaggacaat atgccatcga tatgtttatc ggttcaacct tgcaattaga ttacgaagga
    13141 aactcttcta ccgtaacaaa aggacgttta tctggttttg gcggtgcgcc taacatggga
    13201 cataatcctg gcggtcgtcg tcattctaca ccagcatggc aatcacttcg cgacaatgat
    13261 gatccattag ggaaaggcca aaaattagtt gttcaaatgg ttgaaacata tggttcaaat
    13321 aaaaaaccag tctttgtcga agaattagat gcactagctg ttcaaaaaca agcaggctta
    13381 gccaatgcac cagtgatgat ttatagcgaa gatactacac atatcgtgac agaagaagga
    13441 attgcttatt tgtacaaagc aaaaacaaaa gaagaacgac aagcagcaat tgcagcaatt
    13501 ggcggtgtta cacctcttgg aatgactagc acgaaagaat cactagatag cttgcgtgca
    13561 gccggcatcg tgaaattacc agaagatcta ggcatcaaac gtagtgaagc gaaacgttca
    13621 ttattagctg cacaaaccat cgatgatttg atcgaatggt cagatggcct gtatgaacca
    13681 ccaatgaaat tcagaacttg gtcataaaat tggcgagaaa aaccttttgt cgccatttac
    13741 ccagatggaa gaagaaagac gaataagcag aacgtgtatg ctctgtttta caaaaagggg
    13801 agagatccat ggaacaatta gcttataaat atccaacgaa acagccgatc aataagaaaa
    13861 tccatgtagg agtggtcggt tcaggagatt tggaaatctt gatgtttcca acagaaaaaa
    13921 cagaatctac tgtaaaaatt tgtacaggaa gcgatggctt cggcgaagta tggaacaatg
    13981 tattaacgag attttttgat cgttatccaa tttcagcaga tatcgtgatc aatgacttcg
    14041 gtgctacacc aggagttgtg tacctacgat tgacgcaagc aatggaggaa ctttcagatg
    14101 aaaaatagtt ttgtagaatt aaacgccaga gaacgagtga acgctctact tgacaatgga
    14161 cgtgaattga ttgatccatt cgatcaaatc atcgcgccaa gtttaccagc acaaggaatc
    14221 gtccctgaaa gtgatgacgg gatcgttgtc agccgtggca cgatcaatgg caaacaagca
    14281 gtagtaatcg ctatggaagg aaaattccaa ggtggcggta tcggtgaagt ttccggtgca
    14341 aaaatcgttg ctgcacttga acatgtgtta gaagaaaata aaaaaggaaa tgagatcttt
    14401 ccagtcattg tactagatac aggtggtgtt cgtttgcaag aagcaaacta cggactgtta
    14461 tctatttcag aaatcggaaa tgtaatcatt gctctaaaac aatatgtacc agtcatcggt
    14521 ttgattccag gacgtgtagg atcatttggc ggtatgtcga ttacttcagc attaatgagt
    14581 tatttaatcg caacaaaaaa agcgagagtc ggtttgaatg gaccagaagt tattgaacaa
    14641 gaagcaggcg ttcgagaatt tgattctagt gacaaagact tgatttggaa tacattagga
    14701 tctcgtcaac gggtagaatc aaaaatcatt gatgaattag tgactgatag tgtagaagca
    14761 atcaaagctg ctgtcatcgc agcaatggac gaacgaaaag acacgcatcg cagtgaaagc
    14821 tcagcatttt atttatcatt acttgattct ttagatcttt caaaaccaat ggccattgac
    14881 gattacaatg aagcaattgc taaccatcaa gcaaaatcaa ttgacttacc agaagtagca
    14941 gaaggaaatg aaccagctgc agcaagtgtt ggttacgatt ggtttgcggc actaactggc
    15001 atgaaaaatc caacttcttc tattagtact gtgtattatg gtaaatcaag taaattcata
    15061 gaagaagcca tcgtgacatc aatcgtacct gatcctaaaa acccgatgta ccgtgtacga
    15121 aacggtgaag ttggtttagt tgaaggtttc cgcatgggac aaattttgga tcacgtttac
    15181 gaagaagata aagacaaaaa agtgaaacgg ccaatcatcg ttgtgatcga tgtaccaagc
    15241 caagcatacg gctataacga agaattgatc ggtatccatg tggctttggc taatagtgct
    15301 gctgcttatg caaaactccg tcaagctgga catccagtta ttggattgat tgcaggaaat
    15361 gctatttcag gtgcattttt agcacatgga ttacaatcaa gccgcttgat tgctttaaac
    15421 aataaagaaa ttaccgtcca agcgatgtct aaagaaagtg ctgcacgtgt gacaaaacgt
    15481 tcggttgctg aaatcgaagc agctgctgaa aaagttccag ctatcgctta cgatatcaat
    15541 aattatacaa aactaggagc gatctatcat ttcctagaag atatcaccga ccaagctgct
    15601 tcagaacaaa atgtcagcaa agtcatcgaa gcaatcaatg aagccattca agatgtccgc
    15661 aatacaaatg acacgatgct gacaaatcga tacacaaacg aaacagccaa aacattcgga
    15721 cgtgtcgaaa caaataaagt ccgtgccaaa atggacgaag aatgggatgc ataaaaaatg
    15781 attcaagcac atgatattgt cctttttgat aaacatgaaa tagaagggtt caccttgcct
    15841 gaatgggtcg ttctaactga aaagatgttc ggaacagttc gtcgtgcgtc gacggatcac
    15901 gcagaaatac taccgattgg tttacgtgga cctgcccgca atcagagatg gtcaacagaa
    15961 atctcaaaac atgccgtcaa acaagtcatt catccgtggg acatcatcga acaagaatcg
    16021 tatagacaag agaaaatcat ggattatcct gtttatcagc aatttcatgc tgctagaaac
    16081 atattatcag gatgtaaatg gggaatcggc ggcagcttag gatttgaatt atcgactggg
    16141 attccggcag taaaagaaac cagtgatttt gatttgctgc tgtacgcaaa ttcaccgatt
    16201 gaattgccaa ttcaggtaat tcaatcgcat cctgcttttt ttgagcaatt cgatacccaa
    16261 gtagtgactc ggcaaggcgg tttttcacta aaagagtatt tgagagagcc agagaaaaaa
    16321 attctattga aaacagtcga aggacctaaa ttaaccaaag aactttggta ataaagaaaa
    16381 agagagagaa agagatgttt gaaaacaaaa aaatcaaaat caacgtaagt gacttccaga
    16441 aagtatcagc tgtaacggct gttatgatgc aaacgatatt gagctttgct ttggcaaaca
    16501 gccacagcgc aacaaataca gcttttcttg gtacctttta cgtcatcgtt aaatacacag
    16561 caccaatgtt tatttttgca attgtttata atatggtaaa aacaagtcag catttgtctt
    16621 attgggaatt tttgaaagaa aagttttttg aactagtcgt tccgtatctt ctatggacga
    16681 cggcttatct ttggctattt ccagcagtcc aacaacaaac tccgtatacg gatactgcaa
    16741 gctttttact gaaatatgtc acaggtgatg gagcgcctca tttatggtat accgttatga
    16801 tgctgcagat ccaattgttc atgccgtttt ttgtttggct cggttacaaa gtatttgcta
    16861 ataaaaaata tgtatggcca gtattgattg gttcaacgat tttatatgtc gcatggtatc
    16921 tgttctatca aacacaagta ttgactgggc cttatcatga cagctggtac ttattagatc
    16981 ggtttgttgc tagtttcatg atttatgggg tagccgcatt tgtttatcac gaaaaagtct
    17041 atcaatattt ggacagagta cgttatcttt tcttacctgt tgccttagtt atcgcgttct
    17101 tttctgtccg ttcattgctc gcacatccag gagacttgag ctttgcgaat gcaccttact
    17161 tgaatacgat ccaaagttta tacagtttag tcatcatctt tgctgtcttc ataggcgctt
    17221 ccaaaatgat tgtaaacgat tcgccaaaat tgccattgtt caaatggttg tctgtttatg
    17281 cttatcgtac gtatttagcc aatgtatttg ttttccaagt actgttatta ttgtttaaag
    17341 atagttggct gcagttacct tcaggagtca tgattcttgt agcttatcta atgacggcaa
    17401 gttgtgcatt tgctttaagc tggttacttc atatcatttg ggtagctatc aaaaaaggtt
    17461 tcagcaagta atagtaggaa gaaggagtga gtgtcatgat aaatgacgaa cgaaaaaaag
    17521 cagtgactca gcaactcgct gcgttggtag aggaagcatt gatcgcagaa gtaacattaa
    17581 gtccaaaacc tggattagtg gatgcactgg atgcgggtgc acattccgat atggactatg
    17641 ctttatttct taagtctgct aagacgctaa ctcctttttt tgaagaaatg gctcaaatag
    17701 catggcatca tgctattgat caggagttaa gagaaagaat tgctgagatt gggcgaaaag
    17761 cggaaaaggc aatgctgatt gctacaaatg gcatcaatac ccataaagga gcgatttggg
    17821 caatggggct attgatcagt gtcattgcac aaaaaatcag tatggaacag cctttttctt
    17881 tatcgaaaat acttgatgat gtcagcaagc ttgctgcttt tcctgataca aaatatcgtc
    17941 ataaaaaaac cactcatggt catagggtga agcaaaaata tggcgtaaat ggtgcatatg
    18001 aagaagcgat attagggtat ccgcatatca ggcttgcaat tcaagcagct aaggagctag
    18061 ataatgattc atatgtacag aaacaaatgc atatgttgtt agttttgatg agttcgctag
    18121 atgacacatg cgtattgcat cgcagtgatc agaaaacgtt agtggaaatg caaaaacttg
    18181 ctcatgctgc tagtcaaagt gctttaccaa atcgtccatt tacagcactg ttgcactttt
    18241 gtcaggaaaa ccaaatctct cctggaggaa gcgcagatct cttgtctgca agcttgttca
    18301 tgctgaagat agaggaatta tggactgccg acacatacat ttgtccgatg ttactcaatg
    18361 actaaataag acaaaattgc taaaaaaaca gtttatgcat ctggttattg actcttcctt
    18421 ttagcaaggc tatcataaaa atagtttttt gtaataaaag ataacgagga agacaatgaa
    18481 aaaaatcaat tatttagcaa tcattttgtt agtgggccta tgtttgtttg ctctgccgac
    18541 ccaaggattc gctgtcgcgg gtggtcatgg tggaggtaca tctagcggtc gaagttcggg
    18601 cagaggttca tttggtggtc gttcatatgg gggcagttat tttggcagtc gttcttacgg
    18661 cagagattct tatgggtatt atggtccagg tacaggtcct tctagttatg agcaaacggg
    18721 atttcttgca ttagctgttt tgtcagtagg aggggtttct gctttttcta tcgtcaagaa
    18781 taaaagacaa aagaaaaggc tgaaagcgga gatattgtcc cgcatgtcag gaacggctaa
    18841 agaaaagaaa gcacagtttc tggaaatcca aaagatattc atgaagatcc aatccgcttg
    18901 ggaagaagaa actcctgaaa aagccagaag ctgttataca gacaagctgt ttgccgaaca
    18961 tcagaaagtt ctgcaaaaaa acaaagcaga cggtgtaaga aatcatacta aaaaaataac
    19021 tattcaggaa gtaggaaatt atcgacagat ccacgaaaat agttttgcca tccgaattga
    19081 tttcagatgc tgtgattatt cagtcgatcg aaaagatggg cgattgatct caggaagtaa
    19141 gacacaacga cagtattttt ctcagatatg gtattttgat ttttctgaga aagaaaaaag
    19201 atggcaagcc gattttattc agccgattat gctggattga caggaaatat ttatgaacgg
    19261 agttgtgaca aaagccttgt cgcagctcct ttttctgaat aaacggtgtg ccagagatag
    19321 cctatcggca ataaggcacg attttgtaaa atatgggaag cgatttttca aaatcgcttc
    19381 ttatcgctca aggctgaaca tctctgtcac aacctctcca ctaaatggtg tttaaaagct
    19441 tgcatctgcg ataataagaa caaccaaacg aaaaatagga agcactattt tagtttagtt
    19501 gttcttatta cttgatgcag gacagctttt tcacaacctc ttttttgtat agatgggcaa
    19561 aatttttgta ataataggcg tatgtctttt tcgttgagga gatgaaaaag catatggctt
    19621 attttttgtt tgctttgctt actttctttc cacttcaagt ggcagaaata tctacttttt
    19681 attatgtaag cggaaccata tacttgaaat cagaaggaga tcatttctat gaaaacaaga
    19741 acaattgaaa tacttcacag aatcatgaca gcccaatatc cagtaaaagc aaaacatcta
    19801 gcaagtgagt tcaatgtcag tcagcggacg atccgtcagg aagttcttga agcgaatatc
    19861 tggttgagca gtaaaaagct tccggagatc cgtacaattc gcaacaaagg ttttttgttg
    19921 aagctggaaa aggaaaagca agctcgatta gaaactgaaa taaaagatgt gaaagacggc
    19981 tttttgaatc ggcgcgaacg tatttttgat ctagttttag ccgttgctta cagccgaatc
    20041 ccgatccatc taaacaaaaa agaagaagaa tttcaaatct ctaaaagtac attagatgaa
    20101 gatatgagac ggttacgagc aacactggtc agttatggga tagagatcgt cagttatgga
    20161 aagcaaggac tggtttataa aggttcagaa cgatctatcc gtacgatgat ctatgatttt
    20221 atcaatcaga atctgggaat gaccgacttc tttatgaatc aagatgcaca attaacaccg
    20281 gctcaaaaaa tctttctcca ttattttcct ttggtggaaa tcaaaaaaat acaggagatc
    20341 tattccaata agttgttgaa acaagaagat gatatttata aaaaacaaat cctgcttttt
    20401 acattgatct ggatccagcg aatcaggaaa catgaattta tctctgccat taattggaaa
    20461 aatacagaaa taaaagacag tagcttttct gcatttattg accacatgat cgaggaattt
    20521 caattggaca atgtcccgca agtagaaaga aattatcttc gtttcactat tgagacgttc
    20581 aatgctagag atatctccaa ttcaatcgag tgggtacaag ctcaattact aacgatccaa
    20641 ttgatccaat ttgtagagaa cgagacacac gtacctttcc atctaaagga agaagcttta
    20701 ttcgaaaatc tctataagca catggctgca ctgattgtaa gaatcaagaa tcatatccaa
    20761 gtcatgaatc cattgaaaga aaacatccga acaaattatt tcccaatcta ccgcgcagtc
    20821 actcattttg ttcctacgat cgaagaagta attggtggca agatactgga ggacgagata
    20881 gcttttcttg tcatccactt ttcgacgatc gccagtgtaa tcaagcgaga cttgaattat
    20941 gtttataaat cagtcgttgt ctgtaaccat gggatggcta ctggaaatct gttagcagag
    21001 aatctgaaag aaaagtttcc tcagatcgag attactgcag ttttaagttc aaaagaaatc
    21061 gaattagtag ataaattgga tgtcaatctg gttttcagca cctttcaatt gaattatcga
    21121 aataagccgc ttttagttgt cgatccgatt ttaactgaga cgaatatctt ggttatagaa
    21181 gactttttga aaagaaatga agaactacag cggttagagc ctaacagtca tgaatcgacg
    21241 gagttattca ctcaaataat agatgtcatg aaagaaagtg gtggccgagt agatcgaatc
    21301 atctatggca agttagaaaa attattcgaa acaaacaatt tagaaatcaa caaaagggag
    21361 atccagccta tgttaaaaga tattttgacg gacagcaata tcatgatcaa agaaaaagcc
    21421 actacttggg aggaatcgat tgaactagct gctagaccat tggttaaaga aaacattgtc
    21481 gaagagcgct atgtcgaagc gatgattcgt gcagtgaatg attacggacc gtatatcgtg
    21541 attggcaaac acatggcact agcccatgcc cgaccagaag atggggtcaa taaattagga
    21601 gtcagcgtag ctacgattga gcaaccaatc gatttcggta atcctgaaat ggatccagta
    21661 aagattattt tttgtttggc agcagtagac tcctactcgc atctgaacat catgaaagaa
    21721 ctaattgaat tgatcaacaa cgaagagaag ttggatcgat tgattgaatg cacaaatatc
    21781 gaagatttca aacaactgct tttttcagta gttgagaaat aataaatgat acgggggaga
    21841 acaaagatga acaaattgaa cattttattc gtatgcggtg caggtttagg aagtagtttt
    21901 gcagcacaga tgtctgctga ggatgttttg aacaaattag gagtagaagc caaactggac
    21961 cattgtgata tttcttccgc agtttccatg aaaccagatg tgatcatcac agcagaaaat
    22021 tttcgttcgc agttcgaaaa gttcgcagtt gatcctaaaa cggcgatgat ctatttgaaa
    22081 aatattgttt caaaaaatga aattgaagaa aaaatcaccc cagttctgca agataaggga
    22141 gttatctaaa tacaaaaaga aaagagggta aaaa
//
LOCUS       DAZKCC010000047        20791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000047 DAZKCC010000000
VERSION     DAZKCC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.047"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1610)
                     /locus_tag="ACG8VH_001779"
     CDS             complement(27..1610)
                     /locus_tag="ACG8VH_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110335.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1732..2316
                     /locus_tag="ACG8VH_001780"
     CDS             1732..2316
                     /locus_tag="ACG8VH_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110336.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2327..2572
                     /locus_tag="ACG8VH_001781"
     CDS             2327..2572
                     /locus_tag="ACG8VH_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110337.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2578..3300
                     /locus_tag="ACG8VH_001782"
     CDS             2578..3300
                     /locus_tag="ACG8VH_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110338.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3431..4174
                     /locus_tag="ACG8VH_001783"
     CDS             3431..4174
                     /locus_tag="ACG8VH_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110339.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4180..4545
                     /locus_tag="ACG8VH_001784"
     CDS             4180..4545
                     /locus_tag="ACG8VH_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110340.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4561..5091
                     /locus_tag="ACG8VH_001785"
     CDS             4561..5091
                     /locus_tag="ACG8VH_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110341.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5253..5561
                     /locus_tag="ACG8VH_001786"
     CDS             5253..5561
                     /locus_tag="ACG8VH_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110342.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            5668..5976
                     /locus_tag="ACG8VH_001787"
     CDS             5668..5976
                     /locus_tag="ACG8VH_001787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110343.1"
                     /translation="MYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMS
                     RFLKEREKKELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ
                     "
     gene            6113..6913
                     /locus_tag="ACG8VH_001788"
     CDS             6113..6913
                     /locus_tag="ACG8VH_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110344.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6950..7132
                     /locus_tag="ACG8VH_001789"
     CDS             6950..7132
                     /locus_tag="ACG8VH_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110345.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7243..7560
                     /locus_tag="ACG8VH_001790"
     CDS             7243..7560
                     /locus_tag="ACG8VH_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110346.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7563..7706
                     /locus_tag="ACG8VH_001791"
     CDS             7563..7706
                     /locus_tag="ACG8VH_001791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110347.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(7910..8389)
                     /locus_tag="ACG8VH_001792"
     CDS             complement(7910..8389)
                     /locus_tag="ACG8VH_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110348.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8473..9846
                     /locus_tag="ACG8VH_001793"
     CDS             8473..9846
                     /locus_tag="ACG8VH_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFP9110349.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            9869..10396
                     /locus_tag="ACG8VH_001794"
     CDS             9869..10396
                     /locus_tag="ACG8VH_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110350.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10383..10946
                     /locus_tag="ACG8VH_001795"
     CDS             10383..10946
                     /locus_tag="ACG8VH_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110351.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11368..12105
                     /locus_tag="ACG8VH_001796"
     CDS             11368..12105
                     /locus_tag="ACG8VH_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110352.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(12122..12538)
                     /locus_tag="ACG8VH_001797"
     CDS             complement(12122..12538)
                     /locus_tag="ACG8VH_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110353.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            12931..14178
                     /locus_tag="ACG8VH_001798"
     CDS             12931..14178
                     /locus_tag="ACG8VH_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9110354.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14446..16836
                     /locus_tag="ACG8VH_001799"
     CDS             14446..16836
                     /locus_tag="ACG8VH_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9110355.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16885..18720
                     /locus_tag="ACG8VH_001800"
     CDS             16885..18720
                     /locus_tag="ACG8VH_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9110356.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18795..19652
                     /locus_tag="ACG8VH_001801"
     CDS             18795..19652
                     /locus_tag="ACG8VH_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9110357.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            19642..20751
                     /locus_tag="ACG8VH_001802"
     CDS             19642..20751
                     /locus_tag="ACG8VH_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9110358.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
BASE COUNT         7666 a         2925 c         4045 g         6155 t
ORIGIN      
        1 aaaagtgcat cttgtgttat aaaaaattat tttattttgt gaacataatt taacaattct
       61 ttctcggaat tgatttcttt tcttaaatag tcttcagact tattaataat attatctatt
      121 atgtctagat acatatcact tttcgattca gctaattcat acagtttttt taattcttta
      181 tcctttaatt gaaaaccctg atccatagtc actgcatttg gatatagaag cccactataa
      241 tgtttacgta aattcacttg gaatttctga ttataaaagt ctaacagatt agaaataaaa
      301 ggcggaaaat tactgttttt gtaaaattga ttatttccat ttaggttgta tagtaatttg
      361 ttaaattgct cctgatttaa tttattgtaa atccatggta atacttccat atccttatct
      421 gctaacctga atgaactagc atcggtaaga tgttcgatca taaaatctat gaacatctct
      481 ttttcaccaa aatataaata ttgattatat attgtttcta atgttgaaga tggtagcgaa
      541 gaaactctca ttttttgctg gagtatagga tcaactagac tatttaagtg attttgtaat
      601 tcttcattgt tgtttcctag aactgcaagg gaacgtaaac ggtagtaata ttctatttct
      661 gttagtactt gttgtttcgg tttaaaatca tttattgata tttgtaaatt ttgaattgtc
      721 tcgtctgaaa aatcatgttg taattctgga tgggtagcta taaaagtaag taaaaagaac
      781 ccttttgtat acgtgatctg ttgattttca ccaagtgatt tattaatttc ctctcttgat
      841 tcagctgtga acctatactt tgcaatttct ttttttagaa tatttaaata gaattcttta
      901 ttatcaaaaa ttagggcatt aagtagtctg aaagtacttt ccctatctct atctgcttcc
      961 tcatttccat aagataattg aaaaacaaac ttaaaggctg tcttacatag gttttctttg
     1021 gctttttcgc ttagtttatt tagatagcta ttttttagtg cagagtgaaa ttcttcaggt
     1081 gtaacagaca tagcatgtat gcgacttgca tattgtataa tttcgatcgt tacttttttt
     1141 cctaatattg gattttttag gaatataatt tcataggact tccgtataac atcctgacaa
     1201 gtttcttttg ttatatctct taattcccaa gtattaccgt tgtaagtgac tacaggatga
     1261 gctgaataat ttctttgatc cttaatatat tttaaatcct catattcata gctatcaatt
     1321 agctcagttg aactataagc tttttctagt agttccattt cccattttgg agagtttgga
     1381 tcactttttc ttagtctagc tatatcttct aaaattttct tagaagtatt atcctcgtaa
     1441 atatttgcta aatcagataa tttatcaatt aaatctaaaa taagcgcact gtaattgaca
     1501 gctattgcag aacgataatt tccctgataa aaactacttc taacttcttc taataatgga
     1561 ataattctag gatttttaac tttttttatt tcatcatcta atataaacat ataaatctcc
     1621 tttttttatt tgtatgacaa gtttaccata aaaaaaaatt aaaaagcgag acatatttat
     1681 gttgttgtgt tatcataata acataaatat gtagagtgag atgatgaaaa aatggatttg
     1741 aaacaatcgt tttacatctg gttatatcct gataatccta tgtcaataat ggctgatatt
     1801 tttgtagtta gcatcttatt gtatttattt tatctaattt tctttcacaa tttaatcggg
     1861 tacatgattc ttgattggaa aggaatgttc gatatagaaa gaaaaccatt tgctaacaat
     1921 caaacctata ccgtcttcga aagtggagat tgggaagaaa atgaagttga tgtgatccat
     1981 gaaattggat tagctttatc aggaatacaa gatgctgatg ttttggtatc aatgacaacg
     2041 gatactggat acaacgctta tgaggtattt tatcttatgt attttatatg gcgtgatggt
     2101 caagaaatta tcaatggtcg tgctgaaaaa acaatcaatt ctaatactga aatttctttt
     2161 tgtatgggtg aaggggaaat gattgtatat aaagatgggt cagcaaacat taaaacagcc
     2221 tttggtgata ttgaaacaag tattaaggaa cttagggaat atgtgcagaa cccatataat
     2281 tttcaatatt tagcaggaag aataaattta gtttaggagt aaaaatatga aaaaaattat
     2341 aaatcaacca acaaatcact tgataattga aggtgttatt ttcgtaacaa tgtattttac
     2401 tgtaggaaat ggcatctcaa atacaatcat tcaaaaaaat ccacaatacc tattatttag
     2461 tctttatgct tggattgtac aaatggtttg ggtttccata ttgatgctgt tgttttattg
     2521 gatagtacgg gttattcttt tattgttaaa caaaagaaaa aaggataggt gaataaaatg
     2581 aaatcaacta aattgaaggt tgtattgtta tcgacagcag cattaataac ccttacagca
     2641 tgtggtacaa ctaatagtgc taataataac aaagacacaa agtctagtag tacgatggtt
     2701 actaaaaaca gcactacatc ggcatcaaca acaatgtctt caatgactga aaataatgct
     2761 gaaaagtatc aaaaatacga agacattaat aaaggtcaaa agccggatga agtttataac
     2821 aactttatta atacagtaaa aaatgatgaa aattatggga atgatccatt gcaaaataaa
     2881 aatctgacta tcgttcttta caaacctgat tgtccagatt gtcaaaaagc agaatcaaaa
     2941 atcatgcaag aaataaaaga aggatttagt tatgctccaa cattaatgca tgatattacc
     3001 ataaataaac cagcttacga gtattacgca gttgctgtag atattactga tggtgtacct
     3061 gagtgggtac aaaacattcc tggtctggct gatgagaaca atcaatatca tacgccaaca
     3121 gttgcagtag taacaccaaa gaaagttaac ggcacactat actgggatgt aatgggaatg
     3181 acaacaaata caactgatga aatgattgaa agtacaacta aatattatat ggatttggga
     3241 gcatataaat tatatcaaca atcagagcct gttatggacg atcaatccaa agttaaatag
     3301 atctttaggg cgtaagccct atttttttaa aattaagtga taacaaataa atgatttctg
     3361 aaacaaacgt tcttactcaa ctactcgaaa cacacaagct aattaactag attataaagg
     3421 agaatgcgta atgaaagaaa tcagtgcaaa tgaagctcat attccgcaaa agaatcaggt
     3481 tggtatatat aaggtgcttc atggtgaagg aatttataaa atacagttac ttaccggtta
     3541 caataatggt gcaaatggga agtggttaaa ggcaacaagt attcgtgaac ttagaagtga
     3601 agtagaaaaa attaggcttg gtctcacaag tgaagaaata gctaaactga agggaaataa
     3661 ttcttttgta attgaaaaag aaaaggaaaa acatctactt tatcgtgagc tgaaaattga
     3721 agaatactat gaaattgatt tacctgaaaa attaagaatt attgatcaac gttcagaaga
     3781 aaaatggaaa actgaaatat atccaagctt aaatgactac aagacatcag gaaagcccat
     3841 gtataactac acaggctttt taacctacac acaatacaaa tatgcctttc gcaaattaaa
     3901 aggcattaaa caagctgaaa ttgccctgta tgagatgaaa aaagtggata gattgaaatt
     3961 gattcaagat ttagtgcaaa atacatggaa tcaagcgatt gtcgagctag aaaaagaaaa
     4021 tgggaaaatt ttaactaata aatatctgat cgtaaatgaa attgaagaat taattggttt
     4081 attttcatgt gaaaaaggtg aagtcttctt gaagtcatca ccaacctatt caattttgga
     4141 cagtccgtat cagtttatac aggcaaggag atgagttaga tgtctagctt gaaagatgac
     4201 tatatccaag caaaacaaga gtatgaaaaa atacagatgt tgaaatctga gaaagtcgaa
     4261 ttatttttaa cacaaaaatt agcagaggat catattgcta acttagatgc taaaacttta
     4321 gaggaatatg atcggcagtt taatcagata catcacaata tcattttgct tgttgagcag
     4381 gcaaagcgaa ggtttgaaaa agcggaatat gatctattag atcaaggaat tgtatgcttg
     4441 gataagggat tgcgaagata ttatggtgca gagttaaaaa agcaaaatgt agatagaaat
     4501 gcactaaaac atgaaatctt agaattgata atgcaacgaa tttgaaggga tagaaagcaa
     4561 atgtcacaaa acattagtgc tgtaaaacaa ttaatagaag aagatttaga gaccacagtt
     4621 cagagattag cggaacgaat ccattttgca aattatcaaa ccatctctaa ctggtcaagc
     4681 aggaacagaa atccggaaga aattccaatg aaattttttt atcgaatcgg aaaagaatat
     4741 ggtgttgatc cgataaaaat tttcgagaag accatgattt attactatca tggaacacct
     4801 aacgaagtgt tagaacatct tttggaagag gcaaaagaaa aatcatcaat attagagcta
     4861 tcgcaaaatg acaaagttat tcaagtgaaa tgcgttgatc caacaggaaa gattattgtc
     4921 ggttttgata ttgaaagaaa atccaaaata gattttatta cacaattaaa aaagataaat
     4981 aatttagata ttctaacgct tagaccagat catgaacagt tgtttatgag ttttaaaaaa
     5041 caattgaaag aaatacaaaa aagatataac aaggtgtttc ctaatgcgtg aaaagctcaa
     5101 agaaatcaga gttttttgga taattaacaa agaatgtatc atcttatata ctcttggatt
     5161 tcttagcatg atcttgtttt ggtggttata tttaaaacag taattggaga gggtcaagag
     5221 gatttttatc aagataagga tgtggaaact gcatgtggaa taatagatta aaaacaggat
     5281 tgttattaat agttatttcc tgcgctatga tgattggtat gaggatacaa agggaacaat
     5341 cctattttga agtttctgct aacaacgtaa tagaaaaatg ctattacggt caacattatt
     5401 ggagtgagga agtcagagaa aatatagatc gtgaatatgt tcaaagaatt gtttgggatg
     5461 catattctat caaagattat cctaaaagtt taacgagtag attattttac agtgaaaaag
     5521 ataatcaaaa actctcagat ttgatgatga aaaaagtatg actattatta cgaaagagaa
     5581 acagagtgct atattcgagg ggaaaaagcc tacaacaaaa gtgactattg gggtgacaag
     5641 gcgaagcgat taactgcaaa ttttagagtg tatgaatcag agtttataaa cttgaccttt
     5701 cttaatagtg tttttattga ttatgtaatt actacgaaac gcattggaag ttattcaagt
     5761 acctacgcat ccttagttcc aatgttaaac tcgatgagcc gtttcttaaa agaaagagaa
     5821 aagaaagagc tacagtatat caaagaaatc atgccggatt ttgaaatgca cgaatggcaa
     5881 attgctttat cagattggaa attggcgaat aaggttcgta gaataacacc ttttcaagcc
     5941 aagcgctttt tgagagctat gaaatttgtg caataaaaaa agccaccaga caagttgatg
     6001 gagactaaca caattaccaa cagttgtaat tgtgttagct tacaaattaa ttatggtgtt
     6061 tagtcaatcc caaattgtga aatggcacca ataattggaa aggaaaaatg aaatgtctga
     6121 agatttacga gagctgaagt tacgaaagaa gcagtatgat cagtattttt ccggtttgtt
     6181 gaataaacat attgaagaag gacttgatca tgattttgga aaagatgaat attgtttata
     6241 ttgcgaacgt tacaatgaaa aaatgtctga atatctacag ctgaaaaaag agatacagaa
     6301 attagagaaa aaagtacatg cttggaagta taaagattcc atgtctggtt atgttgaatc
     6361 tcaaaataag ccaagaaaaa agaagcaagc gtatgttcta aagatggtaa atggggaaga
     6421 gttaattgta ttagcgtaca gtgaagaaga ggttcgagaa ctcgttgaaa cacctgtcag
     6481 gaaaaaggtt gaaacaataa atgagataga cgacagccaa tacaatagca tctatattga
     6541 aacaagtcat ggtggatata agaaattagc aactttcctt agaagttcta aagttgggat
     6601 aatttctaaa aaagacataa gtgctgttac cgatagtttt tgtactttat ctgttcctaa
     6661 agcttatttc caatacgata gtaaaagatt tgaacgctta gagaaattct gtagaccgta
     6721 tactgtattg aaaactaaaa aatttaataa aactaaaaga gtttctatac agttaccgac
     6781 tggtcaaatc tcattaggtg aaaaagacta tctgataaaa gtaggaaaag agttaattat
     6841 aaaagtaccg gaacacttat ttaaagaaaa ttttgagata cgtgaaacct tggtatggaa
     6901 agaagaaaaa tagtttggaa gacatagaat ggtataaggg gaaattgaaa tggcagtaaa
     6961 taaaatattg caggtactaa tcatagacga atttggcgga ggagaatggt ttgatgtgca
     7021 gtccatagac tttgaagaag aagtcgtagt tatagaaaat atttacggcg aagtatattc
     7081 atattcgctt gaaggtgaga atattgagga tttacggttg attggtgagt agaaaaaaac
     7141 agaaatatca aaatggagat aaaaattaat agcatgtaaa gatagtcatg cttgggttaa
     7201 aaatgttaaa attcaaaaaa atgagtcaga agggataaaa gtatgaaaag agaggaatac
     7261 aaacaaagat taaacgaact gcttgaagag gacgaaacat taactcatgg ttcgcctgat
     7321 gaaattctat acatgattga taatatggtg atctttggtg gatatgaact tggtaatagg
     7381 tcagttgatc ataatatttt agaatttgat gatgtatcat gggaagaaat tcttgattgg
     7441 ggaattttgg cggtacctga aactaaaacc tatattagtg acactatggt tccttttttt
     7501 gaggaattgg attataaaag acttcccaaa aatgaaaatc atatactggg aggaaactaa
     7561 caatgttaag tgacttattt attttactga tctatctagg tgtaagtgaa attattgcgt
     7621 attttttgtt tgtcattgct aattatggta tttccagtgc aatcgttcaa atgtttagat
     7681 taccagtaag tgaactaaaa aaatgatgag attatcagca tatcttttaa tgtttttgaa
     7741 tgtggtcacg ttaatctttg tagtaatcgg tgtgactttc gatttaatgg aatagtggga
     7801 aatctataat ggaaaaaaga gacacgaatg aacatatttt ttgaatcaat attgttgatg
     7861 gtttttgcga tcagcgctta ccagaaatct aaaaaagagt aatgattgtt taatcatcac
     7921 tctttttttg tagggaaatt gaaatatgtg gagcttgtat tggtttagtt aggattgctt
     7981 ggctataagg agctagttct ttatcaaaac catcaaagta ggttttcagt agcgatttaa
     8041 ctgatcgctc atcttgtttg atttggataa tttcagcatc caaatcggtt gtgagatcag
     8101 gagcttttga tgcgattacc tgattttttt tatcttccaa ctctcttagg ctgtttaaca
     8161 agttgtaaaa agaatcattt tgattgttca ttccaacgtg agttgcaatt ttaacagaag
     8221 tttcgtccgt attgtcatca aaagaactca ttggaatatc aagtagtaac gaggatgttt
     8281 cttttactga ctttttcttt acagcatttt ttgtttttcc atcttttttg ctctttactc
     8341 gagatgtttt tggagctagt ttaggtaaat cacttgtttt atcagtcatt tattagcttt
     8401 tcctttctat taatttatgt tattatatta tcataataac ataaaaaagt acactttttg
     8461 ggggcgtagt tattgaaaaa atctaagaga aagattgcca cattgcttat gttaatggtg
     8521 caatttacag ggattgcaat gagtgtaatc acaataaatc cgcaagtgat acatgctgat
     8581 gggatagcta cttatgatgc acaatttaac tgtactactg aaggtagtgg gtcaggaaca
     8641 gatagcgatg gaaacgatac tggaagtggc gctgtaacag gtgatgtagc caaaaatatt
     8701 gaagatattt acaaatacat gaatggagaa tatggcttat caggtgctag tattgcaggt
     8761 attttaggta actggaaaca agaatcaggt attgatcctc gagcagtaga agggtattac
     8821 ggagatggac agagagaagt tgacttcatc aaacaattta ctgatggtct gatgggtatt
     8881 ggctatggtc aatggacagc tgggcgacat acagcattag ttgattggtc taagaaaaat
     8941 aatggcggtt cttggtggac tacagaagcc caattggact ttatgttcaa aggtgatggg
     9001 ggattcgtag gaatattaaa agattatgct ttaaatgctt ctgatgatgt cgtagaaaat
     9061 gcagtaactt ttcataaaaa ctgggaaatt tcagctgatt ccgaatcagc tgtaatagct
     9121 gtacgtggtg aaaatgcaaa agaaatctgg aagtacatga aaagcaaggg tatggacggt
     9181 gcaaaagatg aaagtaagat caataaaata tccggaggtt caagtaccgg agatggatcg
     9241 aataattcat caagttcagc atcaggaaca gtagaagatg cctgtgcaac tacggatgac
     9301 gctaattctg gttctggatc aattgggaaa gaaggaaaaa ttaacggtaa aaaaggtcaa
     9361 gtaatagcga atatgactta tgaagaagct ttgaaaaaat atgggaaagc gatcacgcta
     9421 ccaaaattca aagaaccaga ttggtcaact tcacctttta acggaatata tcaaggtcag
     9481 tgtacagagt tgacgtgggc ttatatgagc caattatacg atgggaatca accgactact
     9541 ggaaatggtg gttatgtatg ggaaagctac aaatcggcag gagcaaaaat aacgaacaaa
     9601 ccaatcgtag gttatgggtt tagtgcttcg gatggatggt tatgggctgt tggagatgca
     9661 gggcatactg gtgtagttat agcggtgttt gaagatggat catttctatg tgctaacttt
     9721 aatgttccac caacccaagc tccaacaagg ggagtttcgg taacgttgat tgatggagtg
     9781 gacggatcag ataatgtgca tttctttagc ggtgtaggaa aagctaaatt tgaaatcgaa
     9841 aaataaattg gaatgaagga cgatatagat gaatagtaaa gtgaaagata caattatcat
     9901 ttctttgctt tctggtatgt tattagcact gcttgttgga atcctattct tcaataagca
     9961 gaatcaggac atagaagctg aaaaacacag tgtgcatatt gaagataagc tgacattctt
    10021 taaagaggtt aaaccaaatg aggtggatgg attacggaaa gatttagaaa atgatattca
    10081 agaaatgttg aatggatcag ttcaaaataa gtttgcagat caatcaggtg aagaattttt
    10141 gagaaaacta tttattgtaa caggtaaacc accaattact gataagagta gtgagaaaga
    10201 aaaaatcgag tactataagc cttttacttt taagctaaca aactttggaa ttaagacatc
    10261 tagtagtggg ttgtatgaag cattgaccga ttttgaagtt caatacaatg gaaaaaaagt
    10321 gtatgatacc acattaacca tcacactaaa taatgataag aaagtgactg ggggtgcttt
    10381 ttatgcaaaa tcatgaaatc actactgcta aaaaggcgat cggaatactt tccataattg
    10441 tgatcctttg ttgtgtggta ctatttcaaa agaaaaatgc ttatgataat aatgtcaaag
    10501 aattggaaga ccttaaaagt aaacaaatcg aagtagtcaa gaataaccag aaagtgaaag
    10561 ctatgcagga agctcaatac aaagaattgg gaaatgctga aattaaggaa caaagtagga
    10621 aattctttaa tttattttac aactggaatt catgggaaaa atatacgaag aatatgaagg
    10681 aaatacaaaa agaatttcct cagatagtaa agtctagtcc tgttgatatt tctggcacga
    10741 tggtaggcac tggtaatagt ccaatatcta gctatactgc tgattactat acaacaaatc
    10801 aaaaaggtaa aatggttgaa ttgattacac agacaagaaa tactccaaca acagcaacaa
    10861 caacaatttg gcgtggagta acaagcatag gagaaaatca attgttttat ttggaggatt
    10921 tagatagcta tgtaagagca gagtaaaata aaaaaagagc taacggtacc aacgttagct
    10981 ctttgtgctg acgatgataa caaaacctac cgaaaaagga ttgtttcatc acaaacacaa
    11041 acagaaaaga attgataaaa aacccgaacc tcagaatttt tattaatcaa ttcatttaga
    11101 ttataataaa ttgagctgaa cattacaata cgtatatgtt gtaaaacgta aaaaaagtca
    11161 aaagtaaata ctttcttaaa aagtcaaata aaactattaa agacaatata tatggtttat
    11221 ttgacataag aaaatggtct agatgataat tgtaatatag aattgtagac ttaaataaag
    11281 cataaaatac cttttatgat aaggtatagc tcttaatttt aagcagtttt agatataaga
    11341 ttgaaagtaa agtgggggag aataataatg gcagatcata aattagaaaa gttattagaa
    11401 gaaacgtgga atccaaaaga atttagtgaa ttttttatgg agaattttga aactgactta
    11461 gcagtgattg ttaaagatgc tcttagagag caagggtatc ctgaaactgc aaactatata
    11521 aacattaatt ttactttgta tactgaaaat aaaggaactt gggatttttg ggctacttta
    11581 gcaaataagg agttaagtga taaatcagac acaggaattc gtaacttttt tgaatcaaac
    11641 agagatgatt atatgtatgc taacaatcaa aataaattga actttagagt agagtttgat
    11701 gaaacacctg aagaatttat agaaagacaa ccgcctaaag aaaatgtagc aaaggtattg
    11761 gaagatcgtt ggaattcaga tgaaatagtt tcaacaatta gcgaactagg cggacaatat
    11821 gaaccgctag tagaagcggt tagagaagag ctaagactga ataagtttcc tgatgttcaa
    11881 aatattgatg tttctcaaat tgaaataaac gtcaagatca cgaataaact tgattatggt
    11941 agttgggcag atatagcatt agaaaaatac atctactcaa cgttaaaaga gtttatcgaa
    12001 aatagaatgg atatcatgta tctacagcat ccacagtatt taaactttgg tgttgaaata
    12061 gctacacctc ttgaagaatg gaaaatggag caagggcttg actagccttt gctccatttt
    12121 tttattttac aaatgaatac agttcgtcgt ttttaccaag aatgattgtt tgcccaactt
    12181 tgagattgtc aggtgatcct tcagcggttt gtaaggagaa cgttccattg aacttatcgg
    12241 ataccgttat aggtagcttt aattctgatc cttcctcatc tttgagaatt gctgtttttc
    12301 gttcatcaaa ataaacagtc tttattaaat aatttttcgt agtgatctca gcagatttgt
    12361 ctttttttgt agtagtacca gtttctttag aattagaggt attttctacg actattccat
    12421 ctttggcatt tgagcttgtg tttatttgat tgaatatttt cagcgacaag ataacgctaa
    12481 gaatagtcaa tatagaaaca agtgtaatta atactgataa tttccttttt ttgttcattt
    12541 tttcttaaca tccttttttg ttttgattag ttagatattc cttatctaac aagccttttt
    12601 aggctattaa atgaagataa accccaaaaa aatattgcaa ttatgacagt gcaatggtac
    12661 tattattata caataaattt tgcgaaaggg gtaggtctaa atcgcaaaca aaaagaaatt
    12721 gaacctagac aaatacaact tgtataccgc aagggaagca agtttgattt tgggtaaagg
    12781 tagaaattat gttacaaaca taatcaacta ttatccaaat cgtttgccta aaggttcgtt
    12841 caagaaagtg ggtgacaaaa cctatattat aacggaagaa ggaatagaag cattgcgatc
    12901 acaaacagaa aataaaaaca aaaagaagga atgaatatga agagaaattc gcttggtatg
    12961 gttttattgg catcagctgt acttgcgggg ttatcagtac cagtaacctc acatgctgat
    13021 gaatggactg caaacacacc ggatcaaatc caaattacaa caggacaatc aagttatgcg
    13081 ttcaaaaaag gagatactat ttggtcaact gtagagaagc ttaaaagctt aggttacaag
    13141 actactgttg ctgagtttgc cgaattgaac aatgttgatt tggcaaaagg agaacaacgg
    13201 actatcccga ttggacgaac tttcaacatc agtaaagata cattaacagt taaggaagca
    13261 gatggcgaag tagttagtca aactgttcaa aaagataaac ctgatcctat tgaaaatacg
    13321 gagcaagaga acaaaaaatc cgagactaca aaagaatcgg aaccaacagc aaacacttct
    13381 gataagacag aaacaagtgc ttcaagtgtt gatggagcaa gttcatctaa tacaaataat
    13441 tctacaagtg agaaagaagc atcacaggga acaagtgaaa gtagcactac ggaagctgat
    13501 actggaacat caacaaataa ctcacaatca acatcaaatg atgcaaatga atcaaccagt
    13561 acatcttcta gtgatcaatc acaaacaggt tcgagtgatg aaactgacag ttctacttca
    13621 ccaagtaaac ccgaacaacc aactaatgtt gtgctaggcg ttccatttat tagtcaaggt
    13681 aacacaatgc tttgcgaagg aacatctttg ctagaagcgt tacattataa aggtgttgca
    13741 acaaatcaag atttaatgac ttttgttaaa tcaatgccac tatctcctga caataatcca
    13801 aataatggtt tttctggtga atggagacac aatgtggatg gaacttatca aggtatgaat
    13861 ccggctcctg tagtttcatg gggtgttgct aatggcggaa atgttgtaga tatttctggt
    13921 tcatcggtac aaggattaaa agatgagatt gtaaatggaa atcctgtagt agcgtgggta
    13981 acttatgcct ttgaacctgc tcaacataaa ccaatgtttt ggggcgatgc tatttggaat
    14041 cgacatgtag tagccgttga tggatttaaa gacggttttt accatgttgt cgatcctgtg
    14101 tttggggcaa gttggattaa tgcaggatca tttgaaacag catataacgt atctcaatta
    14161 gcggtttcgg tacgttaata aaagatatcg tttatatact aagtagttat atagtcttta
    14221 tttgtccttg cttaactact tagaaaactt gttgctcatg gtgggtagaa ctaaaagggg
    14281 tgatgctatg aatactagag cgttgaaaca gcttgctttc aacgaaaaaa atgattaata
    14341 ggtgtggtga ttttaactta ggaatgggcg cgaaggtcat taccacaccg acatatcgtg
    14401 gctcattttc ttcgcctaac tggtataaag aaggagtaac gagagatgaa gcatgaaaaa
    14461 acgataattt taaaatcaaa aataatttat tgttttgcga ctttagtaat gatgttatct
    14521 acgcttataa tgcctttttc agcaataaga gctgaagcag atgtcacaaa accgacaagt
    14581 gcaaaattta tagaaactgg cgtaaaaaca gacggttata ttagggttaa tatgccgaat
    14641 catccaaatg aatggatgat ttcaagtcaa tttaaagatt ctcatggaaa tattggatat
    14701 tgtatggatt cagaattgcc atccccaaca ggaagtggag ccggaagctt gaagtacaag
    14761 ggagctggca gtgatgagtt ctatcgtatg tttaaaggtg gttttccatc aaaaacagca
    14821 aaggaactgg gtgcaggaaa tgatacagaa gcatggtatg caacacaatt agtttcatgg
    14881 gttttagccg gaaactttaa agtttcacag attgtatggt cacatccaaa ccataccgcc
    14941 gcagaaacag ctcgtgtaaa aaaagcattt gaaaaaattt atgactatgc taagaatgga
    15001 aaagatacgc ccaacactga gttttctatc acagcttcta agacagctga cgaaggaaaa
    15061 tatcacactt tcacatataa aacagcatca aataagactg gtaatgcgaa gctcactttt
    15121 acatcagcaa aaccagctgg tatgaagatt tatgatgcag atggtaaaga aattactaac
    15181 aacactgtga aattaaattc aagttttact attaaagtac ctgtgactac accaagtggt
    15241 actttgtcat ttaaaggtac tgctaatgtt agcacaacca atccttttac ttttgatgga
    15301 cgaggtgtgt atcaagatgc cgtagttatg atcactactt cagaaacaaa ggactctaaa
    15361 tctttgagtg ctaaatggac aagagctgtt ggtcaggtaa cagttaagaa agttgatcaa
    15421 gatggaaatg ctttaaaagg agcggagttt actctaactg acaataacaa tgctaaatct
    15481 gttaagacaa ccggagatga tgggttagct acgtttgatt tgcaacaagg cttaacatac
    15541 agcttagaag aaacaaaagt tcctgaaggt tatcaaggtt catttaaaaa ggatggtatt
    15601 actgttaata ccgatggaca agaatttagt tacactgcta cgaacacaat taaaaaaggt
    15661 aaagtaaaag ttaaaaaggt tgaccaagat ggaaaaccat tagctaatgc tgaatttacc
    15721 ttgacggatg attcatccca aacacaagca gaaaaaacag atgaaaacgg acttgcaagc
    15781 ttcgatattg tagcaaacaa gacctacagc cttgaagaga ccggagtacc aactggttat
    15841 acaggcgtat tcaagcaaga aggaattacc gttaaagatg atggacaaac ttttgaatat
    15901 acagctacaa acactttgaa aaaaggttta gtaaaagttc ataaagtcga tcaagatgga
    15961 aaacctttgg caaatgcaga gttcacttta accgatgaaa aaggtgcaac acaagtagaa
    16021 aaaacagatg aaaatggact tgcaagcttc aatattgtag caaacaagac gtatagccta
    16081 gaagaaaccg gagtaccagt aggttacaca ggtgcattta agcaagaggg tatcaccgtt
    16141 aaagacgatg ggcaaacttt tgaatataca gctacaaata atttgaaaaa aggtgtagta
    16201 aaagttcata aagtcgatca agatggaaaa cctttagcag atgcagaatt caccttaacg
    16261 gatgaaaaag gtgtaaaaga agttaaaaag acggatgcaa ctggtctggc tacgttcaac
    16321 attattgcga acaaaactta caacttagaa gaaacaaaag taccaacagg ttacacaggc
    16381 gcattcaaac aagaaggaat taccgtcaaa gatgatggac aagtctttga atatacagct
    16441 aaaaacactt tgaaaaaagg tgtgatcaaa gttcataaag tcgatgatca agggaaagca
    16501 ttagcaaacg cagaattcac tttgacggac gacaaaggtg taaaagttgt acaaaaaacg
    16561 gacagaaatg gcttggcaac atttaatatt gttgccaaca aaacgtacaa aatcgaagaa
    16621 acacaaaacc ctaatggtta tacagggaaa tggtctaaag aaggcatcac cgttgctgac
    16681 gatggacaaa cctttgaata tacagtaatt aatcaccttg tacctgaaac agtaagaaca
    16741 ggcggaatcg gtacatcagc cttccttctt gtatttctaa ttgcaggatt gagtatggtg
    16801 ctactgtatt atcgagaaca gcacaaagca aaataaaaaa gtgttaatgg aaaagaagta
    16861 aatatgaaaa aagaaggctg acgtatgaaa tcaaaaaaaa ttttttcaaa tttaatgcta
    16921 tttggtactt tgctaggagt gggattgggc gcaagctcaa tctcttctag ccttggacta
    16981 ggggggcaag tagcatatgc acaacaaaac tataattatc aaggaactgg aacgattcat
    17041 atccataaag taacaaatac taatggggat gggaaccctg tctcagatat tgataacaaa
    17101 gggaaagaaa tagctttacc aaatggtgta aaacctcttg aaggcgttga gttctcttat
    17161 gtgaaactta ctaaagatga agctaaatcc ttgggatgga cggatagtga tactacaaaa
    17221 ggtcaacata ttgatgatgc taaagcacga ttattactaa acaaaaaata cagtgaagta
    17281 agtagaatga agactgattc acaagggaat atagacatac ctatacaagt aaatacagac
    17341 gatttaacag ataacttgta tttgattgtt gaaacaggaa cagtcaaagg tgtagttgaa
    17401 gaagcgtatc ctatgattgt taacgtacct gaactaaacg gatcagatga tcaatacttt
    17461 aattatgatg tacatgtcta tcctaaaaac tatttccaat taaactcagc agagcttgat
    17521 aaaaaagacg aagatggaaa ggctcttgcc ggaactgttt ttgaattgcg aaaagatgat
    17581 ggatctgatc aggttgttgg aacttataaa acagacagca caggtaaatt aactgtggat
    17641 ggtttggaat taggtgacta ttatttccta gaaaccgatt tgggaacaga tgctaataga
    17701 acaacctatt tactagatgg taaaaaattc aaatttagcg ttaaacgtgg ggagactgct
    17761 aaagtagttc ttgatgttac caactacaaa cgacctgtaa ttactaaggt aattgataaa
    17821 gaaaccgttg atactggcga agatgttcaa tatacattga aaccatcaat tcctggtaat
    17881 attggtaatt acgaaaaata tgttgtcaaa gatgttctag gtgcagagtt aacttatgat
    17941 tcatcagaag tgatcgaagg aatgacttta gatactgatt acagtatcga acgtgacgga
    18001 caaacgacaa actatgtctt tactgaatca ggtatggata agcttcagaa acaatttgat
    18061 gcaggggatc gcttctttac aattttgatg aacgctcata ctaatagtaa agctaaaatg
    18121 gatacaccac ttaagaatga tgtttcaacg atctacaaca atggatatga acccgaagac
    18181 gaaacaccag ttgatcacgc ttatacaatc attggtgatc accaattcat taaaacagac
    18241 ggagatacaa gcaaagcatt gagtggagca aaatttgtag tgaagaacaa aattgatccg
    18301 aataaagtta cttacctaaa acaagatcca acaacgaaag ctgttacttg gattgcagat
    18361 aaatctaaag ctactgaatt cgtttccgga tcagacggta agttccaaat tgaaggtttg
    18421 caatatacct atgatgtaga ttttgataaa caaggtaatc caacaatcac agataaaaaa
    18481 atcaatgatt atgctcttga agaagttgtt gctccttcag gatatacacc aactagaaac
    18541 gatgttccat ttgtggtatc gaatgatgat gcttctagcg aagtaacaga catcaagaac
    18601 cttaaaacac ctgaaacagt tcgtacaggt ggaattggaa cagtagtatt tctagtggtt
    18661 gcaggtgtgg caggaacagg gctatatgtt gtctacaaac gagaacaaca caaagcttaa
    18721 tgaaataaaa ttccctagag tgtttttggc tctagggttt ctttttaaaa agtgtgtgaa
    18781 aagtaggtat gagcatgaaa aagaagaata tcaagttgat catcttaaaa attgggatgt
    18841 tgctatgttt aggagtaggt ctatatcctg taattactca ggcttatacc tactaccaaa
    18901 ctcaacaaac actaaactcc tataaggaaa aatatgatga agcttctgaa aaggagaatg
    18961 aagaagtctt caagagatat tcagaagtta ataggaaacg aaaagacaca gaacaaacct
    19021 cagtcacgat tcctgaagat aataacaata ttccgtcaaa tggaaatctt cagaaggata
    19081 aattaggtga attagtgggt gaaattctta taccaaagat taatgtggat ttgccaattt
    19141 atgatggtgt aaacgatttc caactatcag caggagcagg aatattaacc ggaactgatt
    19201 atccaactgg tgggattgga aaccattctg ttatatcagc tcattcaggc attcctaata
    19261 aaaaatattt tacagattta gagttgctca aacttggtga taaatttttt attgatattt
    19321 cagggaaaaa acttgcttat caagtagatc ggataaaaac aattctacca accgaatttg
    19381 aggatttgct tcctgtggca ggaaaagatt atgtaacact tatgacatgt acacctgttg
    19441 gagataattc tcataggtta ctcgttagag gggttagcat accttaccaa aaaagtattg
    19501 ttgagaaaga aaagaagaaa gaagatttaa aggtaaaggc gcagatgaac tggtatccta
    19561 tcgtagttgt aggggtttta ttaataatag ctgtgatagt cattattaaa aaaatagtag
    19621 caaagagacg aggaatgaaa aatgaagaat aaaatactac tttttttgac tcttatttgc
    19681 actttggttt taacaattta tcctgcaaat cagctctatc atcaattcaa aacaaaagag
    19741 aatgtggcgg aaatccaaga gaaaattgag aaaaaacaaa ctccttcaaa agaggaagtg
    19801 gcaaaaaaag tcgaacaaat aaaatcgaac agcggtaacc aaggattaga caatactcag
    19861 aaaacagata aaggagtaga caatactcag gaaacagacg gtggatcatt tgatattcgt
    19921 gaagctgaag gaaaggtcaa tccatacaca ggagatgttt tttcaaaaaa tcaaacgaaa
    19981 gattttgaac catctaccta ttcaggagcg gttggttatg tctatattcc aaaaataaat
    20041 gtgctagagc ttttgtttct aggcgcttca caggagcatt tatatagagg tgtggcacaa
    20101 atgagtgcta ctagtctacc tttatcaggt aaaggtcatc aaacagtaat agcaggtcat
    20161 cgtggcggtt atacgtcacc acaatttctt tatgttgatc aattagtagc cggagaccac
    20221 atttatatag attatcttgg tgaaaaagca gtttatacgg ttttccgaac agacgttatt
    20281 agaaattacg gtcagaattc aacactgact gatattcctg atttagatat gttaacactt
    20341 tatacttgtc atccatatcc tcagaactac gatcgtttat tagttagaag tctaagagat
    20401 gaaaaagcaa aatacatcac gccacaagta acagttggca ataccgcata cgctgatgcc
    20461 attgatagcg agtccgtttc agataagaca gaaactgtta catcttctaa tgatttattt
    20521 gaggatgttg atcgtgccaa caaggaatta gatggaaata atgatcttaa tattccggaa
    20581 caaggtagcg tagagatgca agacagaacg tttgctgaaa aggcaagttc aatatttaca
    20641 ggaattacca ataaacatca gaaaatgctt ttagtcattt ggactgtaat aggagcatta
    20701 gcaagtctaa aggttcttat tcttttaatc aaagagtttc gaaaagcata atgaagatta
    20761 taaaaattga tagagtacag taaaaaggga t
//
LOCUS       DAZKCC010000048        20614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000048 DAZKCC010000000
VERSION     DAZKCC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.048"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..516)
                     /locus_tag="ACG8VH_001803"
     CDS             complement(19..516)
                     /locus_tag="ACG8VH_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFP9110359.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(646..1356)
                     /gene="budA"
                     /locus_tag="ACG8VH_001804"
     CDS             complement(646..1356)
                     /gene="budA"
                     /locus_tag="ACG8VH_001804"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFP9110360.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1369..3033)
                     /gene="alsS"
                     /locus_tag="ACG8VH_001805"
     CDS             complement(1369..3033)
                     /gene="alsS"
                     /locus_tag="ACG8VH_001805"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFP9110361.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3238..3900)
                     /locus_tag="ACG8VH_001806"
     CDS             complement(3238..3900)
                     /locus_tag="ACG8VH_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9110362.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3910..4725)
                     /locus_tag="ACG8VH_001807"
     CDS             complement(3910..4725)
                     /locus_tag="ACG8VH_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9110363.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4987..5430)
                     /locus_tag="ACG8VH_001808"
     CDS             complement(4987..5430)
                     /locus_tag="ACG8VH_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFP9110364.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5564..5902)
                     /locus_tag="ACG8VH_001809"
     CDS             complement(5564..5902)
                     /locus_tag="ACG8VH_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9110365.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5890..6267)
                     /locus_tag="ACG8VH_001810"
     CDS             complement(5890..6267)
                     /locus_tag="ACG8VH_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9110366.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6491..7684
                     /locus_tag="ACG8VH_001811"
     CDS             6491..7684
                     /locus_tag="ACG8VH_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9110367.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7850..8272)
                     /locus_tag="ACG8VH_001812"
     CDS             complement(7850..8272)
                     /locus_tag="ACG8VH_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110368.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8667..8861
                     /locus_tag="ACG8VH_001813"
     CDS             8667..8861
                     /locus_tag="ACG8VH_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110369.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8858..9352
                     /locus_tag="ACG8VH_001814"
     CDS             8858..9352
                     /locus_tag="ACG8VH_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110370.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9514..10686)
                     /locus_tag="ACG8VH_001815"
     CDS             complement(9514..10686)
                     /locus_tag="ACG8VH_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9110371.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10892..11707)
                     /locus_tag="ACG8VH_001816"
     CDS             complement(10892..11707)
                     /locus_tag="ACG8VH_001816"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9110372.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11857..12303)
                     /locus_tag="ACG8VH_001817"
     CDS             complement(11857..12303)
                     /locus_tag="ACG8VH_001817"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9110373.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12300..12815)
                     /locus_tag="ACG8VH_001818"
     CDS             complement(12300..12815)
                     /locus_tag="ACG8VH_001818"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9110374.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(12888..13802)
                     /locus_tag="ACG8VH_001819"
                     /pseudo
     CDS             complement(12888..13802)
                     /locus_tag="ACG8VH_001819"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(13799..16126)
                     /locus_tag="ACG8VH_001820"
     CDS             complement(13799..16126)
                     /locus_tag="ACG8VH_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFP9110375.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            16735..16881
                     /locus_tag="ACG8VH_001821"
                     /pseudo
     CDS             16735..16881
                     /locus_tag="ACG8VH_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(16969..17262)
                     /locus_tag="ACG8VH_001822"
     CDS             complement(16969..17262)
                     /locus_tag="ACG8VH_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFP9110376.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            17299..17484
                     /locus_tag="ACG8VH_001823"
     CDS             17299..17484
                     /locus_tag="ACG8VH_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110377.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(17665..18843)
                     /locus_tag="ACG8VH_001824"
     CDS             complement(17665..18843)
                     /locus_tag="ACG8VH_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9110378.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(18947..19261)
                     /locus_tag="ACG8VH_001825"
     CDS             complement(18947..19261)
                     /locus_tag="ACG8VH_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110379.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            19368..19583
                     /locus_tag="ACG8VH_001826"
     CDS             19368..19583
                     /locus_tag="ACG8VH_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110380.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(19883..20125)
                     /locus_tag="ACG8VH_001827"
     CDS             complement(19883..20125)
                     /locus_tag="ACG8VH_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110381.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(20112..20540)
                     /locus_tag="ACG8VH_001828"
     CDS             complement(20112..20540)
                     /locus_tag="ACG8VH_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP9110382.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6026 a         4074 c         3530 g         6984 t
ORIGIN      
        1 ttgtttcaat agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc
       61 taataaaaga tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt
      121 tactccgcaa tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac
      181 gatttctgat tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc
      241 aacgatcaat gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat
      301 acgtgtgcaa gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa
      361 ataatatcct gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc
      421 taaatttgct aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat
      481 ttcgtatgca tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc
      541 tatggagaag attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg
      601 agatacgtca ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg
      661 gatttctgct gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac
      721 tggaaaatgc tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc
      781 aaaatccatt acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc
      841 cgctgcagca actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac
      901 attgctttgt tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt
      961 ttcttgcttc ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc
     1021 caaaaatagg ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc
     1081 tgcagttttt gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg
     1141 ggtaagttca cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac
     1201 gccatctaaa aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc
     1261 tttttctaat atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc
     1321 taatgtcccg tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg
     1381 atctggtaag attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat
     1441 aattacgggt ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact
     1501 tgtagctcgg attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc
     1561 aaagtctaca cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta
     1621 gtgtccatca ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa
     1681 atcctgggca gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg
     1741 acgcaccaat gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt
     1801 actgaataac aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg
     1861 actacctaca tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat
     1921 aacttctaaa ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt
     1981 taattctgct tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc
     2041 taatgaacct gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg
     2101 ctgcataaaa ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt
     2161 ttccgcattc cagtttcttg cttcatattc gattggatca tagccaatag caataactag
     2221 atcacttcgt ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa
     2281 gaaatgcgct tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg
     2341 caatgctgtc tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg
     2401 catacctact aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat
     2461 atcatctgca gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg
     2521 gacaggtgca tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact
     2581 cttagccata cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc
     2641 tgcactgtat tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa
     2701 cttcaataga tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc
     2761 tgtcgctgtt accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac
     2821 tactcctggc tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc
     2881 gtgacgagtc acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat
     2941 ttttgcacct ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac
     3001 agatttcgaa ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt
     3061 tttaattatt agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt
     3121 caaaagtata gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga
     3181 atgacagttt ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta
     3241 cagatccata gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga
     3301 aggacctagt agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa
     3361 tagtaaaaaa tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg
     3421 tagaacttga tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct
     3481 aaaggaaact gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga
     3541 aaaacgtata aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag
     3601 ccaataaaaa aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg
     3661 tgaaaaactt ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg
     3721 accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag
     3781 gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga
     3841 ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat
     3901 aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat
     3961 cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc
     4021 gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc
     4081 cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat
     4141 gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc
     4201 ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta
     4261 gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca
     4321 caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg
     4381 ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt
     4441 tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac
     4501 aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac
     4561 cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc
     4621 attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc
     4681 taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat
     4741 ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa
     4801 tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg
     4861 taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa
     4921 aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga
     4981 tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga
     5041 taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg
     5101 aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc
     5161 tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag
     5221 caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt
     5281 tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa
     5341 aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc
     5401 gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag
     5461 tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc
     5521 acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc
     5581 gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg
     5641 attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca
     5701 agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct
     5761 tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg
     5821 ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc
     5881 ttgatttttt tacttgatac attccatttt atttctagat aacacacgat catatttctt
     5941 gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag
     6001 ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga
     6061 taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt
     6121 aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt
     6181 aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt
     6241 ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt
     6301 tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct
     6361 attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt
     6421 cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag
     6481 gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc
     6541 tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt
     6601 tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat
     6661 tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca
     6721 aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt
     6781 ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat
     6841 gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg
     6901 tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac
     6961 gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat
     7021 tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac
     7081 caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat
     7141 catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat
     7201 gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt
     7261 aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact
     7321 agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca
     7381 tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta
     7441 tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa
     7501 taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt
     7561 cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt
     7621 cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca
     7681 ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta
     7741 ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt
     7801 gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac tattctttga
     7861 cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac
     7921 tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt
     7981 caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg tagattggga
     8041 agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat
     8101 caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg
     8161 ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta
     8221 aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc
     8281 ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt
     8341 gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta
     8401 ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac
     8461 gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca
     8521 acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta
     8581 agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt
     8641 tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg
     8701 ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc agtaccacga
     8761 tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag
     8821 agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg aatctgcttt
     8881 gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag cagctatcca
     8941 agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg attccacaat
     9001 caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac gacaacaagc
     9061 agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga ggcaagaagc
     9121 aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac aggcctctca
     9181 gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac
     9241 cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag
     9301 cgaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag
     9361 ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg cgttccagat
     9421 agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt ttttcattta
     9481 gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac
     9541 ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga
     9601 atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct
     9661 gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac
     9721 tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc
     9781 gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat
     9841 ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat
     9901 accgataaac caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt
     9961 ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt
    10021 gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa
    10081 agaatctact tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt
    10141 tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa
    10201 ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt
    10261 cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg
    10321 atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc
    10381 cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa
    10441 actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt
    10501 ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat
    10561 gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat
    10621 cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt
    10681 attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt
    10741 gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc gtttgttgag
    10801 cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt
    10861 tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt
    10921 tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc
    10981 ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag
    11041 aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg
    11101 cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga
    11161 ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga
    11221 cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt tttttcatta
    11281 atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt
    11341 gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag
    11401 taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat
    11461 tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta
    11521 taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa
    11581 ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg
    11641 tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac
    11701 cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta
    11761 cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag
    11821 catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta acacgatatt
    11881 tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag catctttccc
    11941 atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg ttacacttgc
    12001 gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg tagcagtgat
    12061 gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt
    12121 tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca aattaaccgt
    12181 aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta ggccgagaac
    12241 gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt tgatgttttt
    12301 cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca agctgtccct
    12361 gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt
    12421 tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat ctgaactggc
    12481 gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa tggtatagga
    12541 tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg actcaatata
    12601 tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa tccatggaca
    12661 gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc
    12721 caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac tagaaattga
    12781 gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt gcctcctgtt
    12841 tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac atcgttatca
    12901 aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt taattttttt
    12961 ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg ttttattttt
    13021 tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct
    13081 agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc gttgatgttc
    13141 tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa atctgtctgc
    13201 ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc gctctttgga
    13261 tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag cacaacgaca
    13321 aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa tcggtaaaca
    13381 gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg
    13441 tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt ttgtgctagt
    13501 tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa ttcatccgag
    13561 acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa gttatccggg
    13621 atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa cattcgatta
    13681 tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc tataaaaaag
    13741 accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt tgtttttttc
    13801 atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca gaaaaaataa
    13861 agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa ggaagcaaga
    13921 gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa ttattgttta
    13981 cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc cggctgttaa
    14041 ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg agatttccaa
    14101 ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca gtaatcactg
    14161 cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta aatgacaact
    14221 tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt gtatagctgc
    14281 cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata ctgctaagtg
    14341 ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca tggattgacg
    14401 ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca ccgctgattt
    14461 gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt gttctggcag
    14521 ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca atttcagtag
    14581 gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga ttccatgtga
    14641 tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta tcattaaagg
    14701 catatttaac gatcggtaca acagaaagac catcatagta cttactgcca tttgcattga
    14761 ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt tttccatttg
    14821 cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc attgctgatt
    14881 ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata gtaggcgtat
    14941 tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta gaaggagcaa
    15001 tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg cctgtatagg
    15061 tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat
    15121 agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt ccagataatg
    15181 tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa aaagttcctg
    15241 tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt gaaacatttc
    15301 ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga atatcatttg
    15361 ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc cctccccatg
    15421 ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg atcgtatttc
    15481 cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta ccatttactg
    15541 tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg attgctttgg
    15601 ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca acaatgactc
    15661 catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg aaattcgcag
    15721 tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct ttctcccctg
    15781 ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc gaggatgtgg
    15841 ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt atttcagaat
    15901 agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa
    15961 cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg atcgaaatca
    16021 taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca aaatgttttt
    16081 gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct cctctcaata
    16141 aaaaatcatc aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga
    16201 tgtatagaaa gtttggggct ttttttctct ttttattcta aggttatttt agtcttgtcc
    16261 tttaatacaa agattttatt tttaaataaa aaatactctt tttttacgat tattgaaaaa
    16321 aaaagataaa acttgattaa ttgaagtatt gcttaaattt ggaagctatg agggaagaat
    16381 aaaagagtta ggacgggatt atctactatt tttctttgtt ttaaatattt gttatctttg
    16441 aacataaaag agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt
    16501 tcaactagct gatcttgtat tcacttaaaa aatggtaaag tacgagttgc tattccccat
    16561 tttcaatttt ctaacgctca ggacaaaata ctgttttcac aacctcattg gaataaaagc
    16621 atatatattg tttcaagaac gaaatcttgt tatcttttta aagggcatct gtccaaaatc
    16681 taattttagg aggtacgttt tggtatttat tcgtacaaat aaaagagaat tatctttatt
    16741 catgtaacgc atccaattga ttttttcatg tacgctcctg gaacgatttt actgacgatt
    16801 gcttatttaa ctgcgaatcg ctcattagct tttatcatag ctatccatat tttgaataac
    16861 gttcttggct ttgtattata gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa
    16921 ccaatatcgc atacttgaac ttttttcaat gagtataaat taaaacttct aattttcaat
    16981 tcccatcatc tgatatgccc gaccgtctga acttcttttg aaaaagccaa atgtgattag
    17041 ataacgtctt tgttcgacat aatcgccact agaaaatacg ttcagcagta tcgaattaac
    17101 ctctgtttct gtataagtca ctccatggtc aaagcgtttc aagacttctt tcaataggat
    17161 gtatttcttt tttgttgaac ttggtacttg ttttactttt ccatcattca tgaatgtgtc
    17221 taaaactttc atcttttctt gctccgtata agtataaacc atcttctttc tccttttgat
    17281 tctaagaata ctccatttat gttagaatat tctggatttt ttattaaagc ggttactctt
    17341 aagaaagata ttatcatagt gctctttttt ttactactac ttataggtag atttattcta
    17401 atccattatt ctcccctacc aataattaaa gaaaaaactg gatcagggtg ttcaaattac
    17461 atgctaagaa aaaagaaccc ttgaatacca agggttcttt ctcagtctga taacaatgat
    17521 taacgacgga tttctttgat acttgtgaaa agtcgttaaa gtattgatat aaaaggattt
    17581 ttattacctc tgactacata ttgactaatt tgttaaaaaa tcgtttttca agaatcctgt
    17641 aataccagtg ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa tcagaggttt
    17701 taccttttca tttgacgtta agtggcgata aattttcgaa gttgtttggt cagtatggcc
    17761 taagcgatct ttcaagactt cccagctaca gtgttccgct actaagcttg ccattgtatg
    17821 acgagctaaa tgagtatgaa atggcttatt aattcctgag cgattcacgc accgtttgac
    17881 gtgatcattt acatactctc gacgataagg aacaccacgt ttactagaat caagatacac
    17941 aaacaaatat gtttcttcta taataaacct gtaatgttgc ctcatctcac ccaaagcctt
    18001 aatcaactgt tgtagctttt ccataactag aggagttact agaactcgtc tagcagaact
    18061 aactgtctta gtagtttcaa ttcgaaaatc cttactctta cctttaactg ccgtttgctt
    18121 atagaacaga atttcatttc ttttgaaatc tatatctcca ggctgtagag caagcgcctc
    18181 gctgactcgg cagccagtgt aaaatagaac atggaaaagt acagacatcg gtaattcctc
    18241 atgaacaatg ccataattca aaaaagttct caattcatcg acagtaagaa acttgtcctc
    18301 aatattattt atggcttctt ttaaatcagt taccgttaac tgatattttg gcattctcac
    18361 agacttcatt ggattttcac ttatcaaatt ctttctttga caatatttaa aaatcatatt
    18421 tagaacttga ataagtgatt gtaaactatt tttggcatag ccaacatttg ttccattaac
    18481 gccacattcc tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca aatcagacaa
    18541 ctttttatca ccaatccatc gaacaataat ttttaattgt tcctctcgct tatcataggt
    18601 tatttccttt tgttcattta acgaacggta atattggaac cattgctcac ctacaacact
    18661 aaacttgtca ttgcttgcaa caattttttt cttaagcctt tgttgtttct ccatttccag
    18721 ataagcgagt tgcgcctcac gttttgaatt aaagccacgt cttgttgttc gatatgcttg
    18781 aaagtaccat gtgatcctac cagtagaact gatttgcttc ttgaatcgat ctgttttttc
    18841 catttattga ttcctcctat tttgaaggaa cccaacaagc agaaaccctt tggaaatctt
    18901 tctatctatg tcatcattgt accgcatctt agaaagaaaa tgtaacttat aaagatcgaa
    18961 gtagtcgcaa tttttctcga tagtcattcg aatcactctt tctcatccat tctaaaacat
    19021 catcacgaaa atagtgtcct cgaatcccgt accaacaggg aggaaactca ccaagtttta
    19081 ctaaacggtt cagtgtacta tcacttatct gaaatagtcg ctttaaatca gtatttgtta
    19141 ataccagagg taaatgactg actgcaagca atttttccgt gtaatcttcc agtttttttt
    19201 gtaaaattga atagaactct atttccagag aattaacatt tgttttaact tccaactcca
    19261 tcacccttct ctctcaaaat caaaacgacc accacggtac cccttaaaat tgtaaaaaaa
    19321 cactccagta ctccatcaaa acggactaga aaagaggaat ttttaatatg aaacaaggaa
    19381 ttgcagacat aaaaataatt aaagaaattc ttgaaaaatc tacggctaat gcaatagcat
    19441 tcggaacagg aatcaacttg agtacagtaa aaaaacttaa aagtggagag agagctgaag
    19501 aaaaattaaa tcttgccgat gctattaaaa tcacagaatt tggcatgaaa aatatgccaa
    19561 ccaaaatcga aatatggaaa tagtagtttt tctgatctat cagctgtttt gccaaagcac
    19621 ataggaatct cacctccccc cagtaatggc gggccgattt ggttacggaa gtatcattat
    19681 cgtagtcgcg attctcatat taaaatttgt cgcttaaaag ctaaccttgt catggcgtaa
    19741 ctagagaatc aaaatctaca tgtactgata gatttgctat tcatttgtca aagagcgcta
    19801 ggctattttc agcctatgga aatcctttat tttgacccga taaaaaactt ccatagaatg
    19861 aaaaggaaag ggaaaaaata ctttaacgag ttgtatcaaa ttcaagtatc ttctcaatca
    19921 attttgtttc cagttgtcta cgcatatatt catctacata cataaccatt cctttttctt
    19981 cttcataaac tggtttcatg gctaaccgtg aaatataatt ttgatatttt gacagaacga
    20041 tggacatggc aattgaatca ccagaagctg cggctttgat tgtcgttagt ggtatgaatg
    20101 gtatatatcg tttattttgc ttcatatttg acacccaaat actttcctag ctcttccaaa
    20161 gccatttgtc gttggtacca aacactgccg agacttttcc caataatatc gccaatttca
    20221 cgatcattaa gaccgatgaa gaaggacata aaaatgattt cttgtctttg actaggtaat
    20281 gctgcaatcg catttgccaa ctcctcattg gaaataaaga ttttcatacc aaggacaata
    20341 aaagtaccac tactattcaa atttggttcc aaataataga actctgtacc taaccctttt
    20401 tgttccaata tttccattga tatttgccgt tggcgaaatc tagcgtactt cttttgaata
    20461 tttaacgcct cattgcagac tactttctta caaaaactgt caaacgtatg ttgaatgtgg
    20521 gtttcctctg agtcgcccat aatattctcc ccctcaaaaa attcattcaa ctcgttttac
    20581 ttctatccct ttccattatt actacaatct agga
//
LOCUS       DAZKCC010000049        20369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000049 DAZKCC010000000
VERSION     DAZKCC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.050"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8VH_001829"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8VH_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9110383.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8VH_001830"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8VH_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9110384.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8VH_001831"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8VH_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9110385.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8VH_001832"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8VH_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110386.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8VH_001833"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8VH_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9110387.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8VH_001834"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8VH_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9110388.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8VH_001835"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8VH_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110389.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8VH_001836"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8VH_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9110390.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8VH_001837"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8VH_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9110391.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8VH_001838"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8VH_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9110392.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8VH_001839"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8VH_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP9110393.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8VH_001840"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8VH_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9110394.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8VH_001841"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8VH_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9110395.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8VH_001842"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8VH_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP9110396.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8VH_001843"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8VH_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9110397.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8VH_001844"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8VH_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFP9110398.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8VH_001845"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8VH_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110399.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8VH_001846"
     CDS             13967..14695
                     /locus_tag="ACG8VH_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFP9110400.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8VH_001847"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8VH_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP9110401.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8VH_001848"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8VH_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110402.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8VH_001849"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8VH_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110403.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8VH_001850"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8VH_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFP9110404.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8VH_001851"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8VH_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110405.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG8VH_001852"
     CDS             16394..16606
                     /locus_tag="ACG8VH_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110406.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG8VH_001853"
     CDS             complement(16639..16833)
                     /locus_tag="ACG8VH_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110407.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG8VH_001854"
     CDS             complement(16846..17028)
                     /locus_tag="ACG8VH_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110408.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG8VH_001855"
     CDS             complement(17025..17165)
                     /locus_tag="ACG8VH_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110409.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG8VH_001856"
     CDS             complement(17655..18020)
                     /locus_tag="ACG8VH_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110410.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG8VH_001857"
     CDS             complement(18060..18362)
                     /locus_tag="ACG8VH_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP9110411.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG8VH_001858"
     CDS             complement(18362..18661)
                     /locus_tag="ACG8VH_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110412.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG8VH_001859"
     CDS             complement(18654..18818)
                     /locus_tag="ACG8VH_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110413.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG8VH_001860"
     CDS             complement(18824..19093)
                     /locus_tag="ACG8VH_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110414.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG8VH_001861"
     CDS             complement(19105..19854)
                     /locus_tag="ACG8VH_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110415.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..>20369)
                     /locus_tag="ACG8VH_001862"
     CDS             complement(19851..>20369)
                     /locus_tag="ACG8VH_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP9110416.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
BASE COUNT         5644 a         4311 c         3175 g         7239 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtac
//
LOCUS       DAZKCC010000050        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000050 DAZKCC010000000
VERSION     DAZKCC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.051"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8VH_001863"
     CDS             291..1430
                     /locus_tag="ACG8VH_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP9110417.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8VH_001864"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8VH_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9110418.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8VH_001865"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8VH_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP9110419.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8VH_001866"
     CDS             3334..4302
                     /locus_tag="ACG8VH_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9110420.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8VH_001867"
     CDS             4415..4693
                     /locus_tag="ACG8VH_001867"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9110421.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8VH_001868"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8VH_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9110422.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8VH_001869"
     CDS             5150..5905
                     /locus_tag="ACG8VH_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9110423.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8VH_001870"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8VH_001870"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9110424.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8VH_001871"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8VH_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9110425.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8VH_001872"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8VH_001872"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9110426.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8VH_001873"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8VH_001873"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP9110427.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8VH_001874"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8VH_001874"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9110428.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG8VH_001875"
     CDS             10642..11184
                     /locus_tag="ACG8VH_001875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9110429.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8VH_001876"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8VH_001876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9110430.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG8VH_001877"
     CDS             12777..13679
                     /locus_tag="ACG8VH_001877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9110431.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8VH_001878"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8VH_001878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9110432.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG8VH_001879"
     CDS             15122..15544
                     /locus_tag="ACG8VH_001879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9110433.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG8VH_001880"
     CDS             16013..16243
                     /locus_tag="ACG8VH_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP9110434.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG8VH_001881"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG8VH_001881"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9110435.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG8VH_001882"
     CDS             17654..17830
                     /locus_tag="ACG8VH_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9110436.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8VH_001883"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8VH_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9110437.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG8VH_001884"
     CDS             18325..18708
                     /locus_tag="ACG8VH_001884"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9110438.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG8VH_001885"
     CDS             18877..19761
                     /locus_tag="ACG8VH_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9110439.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG8VH_001886"
     CDS             19875..20111
                     /locus_tag="ACG8VH_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP9110440.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG8VH_001887"
     tRNA            20199..20279
                     /locus_tag="ACG8VH_001887"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG8VH_001888"
     tRNA            20286..20357
                     /locus_tag="ACG8VH_001888"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKCC010000051        20155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000051 DAZKCC010000000
VERSION     DAZKCC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.052"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1665)
                     /locus_tag="ACG8VH_001889"
     CDS             complement(106..1665)
                     /locus_tag="ACG8VH_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP9110441.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1820..4054
                     /gene="pcrA"
                     /locus_tag="ACG8VH_001890"
     CDS             1820..4054
                     /gene="pcrA"
                     /locus_tag="ACG8VH_001890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP9110442.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4071..6107
                     /gene="ligA"
                     /locus_tag="ACG8VH_001891"
     CDS             4071..6107
                     /gene="ligA"
                     /locus_tag="ACG8VH_001891"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9110443.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6213..6518
                     /gene="gatC"
                     /locus_tag="ACG8VH_001892"
     CDS             6213..6518
                     /gene="gatC"
                     /locus_tag="ACG8VH_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP9110444.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6518..7990
                     /gene="gatA"
                     /locus_tag="ACG8VH_001893"
     CDS             6518..7990
                     /gene="gatA"
                     /locus_tag="ACG8VH_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP9110445.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7987..9417
                     /gene="gatB"
                     /locus_tag="ACG8VH_001894"
     CDS             7987..9417
                     /gene="gatB"
                     /locus_tag="ACG8VH_001894"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP9110446.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9427..10482
                     /locus_tag="ACG8VH_001895"
     CDS             9427..10482
                     /locus_tag="ACG8VH_001895"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9110447.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10593..11966
                     /gene="rlmD"
                     /locus_tag="ACG8VH_001896"
     CDS             10593..11966
                     /gene="rlmD"
                     /locus_tag="ACG8VH_001896"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9110448.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11969..12097
                     /locus_tag="ACG8VH_001897"
     CDS             11969..12097
                     /locus_tag="ACG8VH_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9110449.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12537..12725
                     /locus_tag="ACG8VH_001898"
     CDS             12537..12725
                     /locus_tag="ACG8VH_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9110450.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12803..13816
                     /locus_tag="ACG8VH_001899"
     CDS             12803..13816
                     /locus_tag="ACG8VH_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9110451.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13883..14917)
                     /locus_tag="ACG8VH_001900"
     CDS             complement(13883..14917)
                     /locus_tag="ACG8VH_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9110452.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15032..17257
                     /locus_tag="ACG8VH_001901"
     CDS             15032..17257
                     /locus_tag="ACG8VH_001901"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9110453.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17275..19158
                     /locus_tag="ACG8VH_001902"
     CDS             17275..19158
                     /locus_tag="ACG8VH_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFP9110454.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19139..20113
                     /locus_tag="ACG8VH_001903"
     CDS             19139..20113
                     /locus_tag="ACG8VH_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP9110455.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6784 a         3702 c         4335 g         5334 t
ORIGIN      
        1 aaaaagaaga ggctgggttt tcccagcctc ttcttttttt atagaatcat atagaatcaa
       61 gtagtgtaga tgaatagttg ttgtaaaata gtttttccat gttcgttaaa atgattgctt
      121 caaatttgtg atagaagatt tagcctcgat ttcctgcttg attcttggca agtttgcttc
      181 atcagtcaag ataaccaaaa tatctcctac attcatgatc gtatcaccgt gagtcaagat
      241 ttcctgattt cctctgcgta tagagattag cagcatattt ttaggccaat tgaagtctcg
      301 taccattaat ccgtctaaag aactctctgc cgttactgga aaatcaaaag ttgtccttgc
      361 accttttagt gaggcggtct gagtatttcc aagtagacgt tccaataatg attcgtaaat
      421 tggattaccg ccgagaaggt cattgacgat ataagcaaca agagcagcca atcctaaagg
      481 catcagatga ttaagagagc caaccatttc tgtcactaaa ataattgctg tcagcggtgc
      541 ttttccaatc gctgtgaaat aaccagccat ggcaataacg acaaaatcct tgatgtagat
      601 tggatccatc ccgaaatcaa ccataatgtt tccataaaag ctaccgatta aagcccccaa
      661 tgtcaatatc ggaaggaaaa tcccccctgg aagaccccca ccgtaagaaa tcatggaaaa
      721 aacgaatcga agaacaaaca aaccgataat cataatgatt ggcaaggaag catgtccaaa
      781 agctaaaacg atttgattgc ctccgcctag agtactaggc cataaataac caattggcaa
      841 aatcagcaag aaaggaacga acccgtgaaa ataagctggt aaaggcaatc ggttgtaaaa
      901 ttttggcaaa gccagcaaga cattctgata aataaaacct aagatgccta gaagtactcc
      961 taagatcact aaggaaccat aatatttcaa cggcaaagaa ggaaggtcac ctagatataa
     1021 aacaggtctc aagccgaaaa agtaaagaga gacgaagtta gcagtgattg ctgaagcaaa
     1081 tgatgtcagc caaacaagtg gagaaaagga gtgatggatt tcttctaaaa caaagagtaa
     1141 tcctgcgata ggagcattga atgctgctgc aagtccgcta cttgcaccgc tagaaattaa
     1201 aatcttttct tctgaagatg gactccgaaa caattgtcca gctccttgtc caatggatgc
     1261 gccaagctgt atagaaggtc cttctctacc tagaaaaagg cccgcaccaa ctgaaagtac
     1321 accgccaatg aactttttcc ataaaacaga gaaccagttc attgataatt ccccgcgaag
     1381 ctgtcccact acttgtggaa tcccgcttcc cttgatatcc ggttcatttt tgaccagata
     1441 acccacgaaa aatgctgcaa ggatgcagca aatagctagg ccaattaagt aaatcggctg
     1501 ttcccgactc atttcataga tacttcgaac taaatcgctt agcttttcga tagataatcg
     1561 aaacaggcta acgacgattc ctgtcagcag tccaacaaat attcctttta gaataaaata
     1621 tatctgagtc ttatctaatt tcttgatttt tccttttttt tccattcaaa cgcctcgctt
     1681 tcttttaatt tagcgcgaaa tcaacgaatt gacaaggaaa aatccataaa aagaaacaaa
     1741 tgattttgaa ctagctagtt taatgaaagc caagaaaatg ctataattaa caagattgat
     1801 aaaagaaagg atgttgttaa tgtcatccaa agctgaatta ttaaacggga tgaacccccg
     1861 tcagaaagaa gctgttcttc acactgacgg accattactg ctaatggcag gtgcaggaag
     1921 tggaaagacg agagtgctga cacaccgcat tgcctatttg attgaggaaa aagaagtcaa
     1981 tccttggaac atcctagcga ttacttttac caataaggct gcaaaagaaa tgaaagaacg
     2041 tgtcaatgcg attctcgcat ctggaggcga agatgtctgg gtatcgactt ttcactcgat
     2101 gtgtgtgcgt attttaagaa gagatgttga ttttatcggg tataaccgga actttacaat
     2161 catcgattct tctgaacagc tgaccttgat gaaacggata ctaaaagaac tgaacattga
     2221 tccgaaaaaa tatgatcctc gaagtatatt aggaacaatc tctcaagcaa aaaacagcct
     2281 gcagacccct caggattttg caaaaatgca aggcagttat tacgaagaga ttgcggcaaa
     2341 atgttatgct gcttaccaaa aagagcttca atataatcaa tgtatggact ttgacgacct
     2401 gatcatgaat acgattcgat tatttgaaga acatcctgat tcactcacgt attatcaaaa
     2461 caaattccat tatattcatg tagatgagta ccaagataca aatcacgcac agtatacgct
     2521 agtcaattta ttggcaggta gatttaggaa cctgtgcgtc gtgggagatg cagatcaaag
     2581 tatttacggc tggcgcggtg cagatatgca gaacatcttg gattttgaaa aagattatcc
     2641 agatgctgca gtaattttgc tagaacaaaa ctatcgctcc acgaaaaaca tccttagcgc
     2701 agcaaaccaa gtcattgaaa acaatagcaa tcgaaaacca aaaaacttgt ggacagaaaa
     2761 caaagaagga aacaagatca cttactaccg tgcagataat gaacgagacg aaacccgttt
     2821 tatcgtagac cggatgcaag aagagattcg aagcaatcat cggaattacg gcgactttgc
     2881 catcttgtac cggacgaacg cacaatctcg tgtaatggaa gaaactctgt tgaaagcaaa
     2941 tattccttat aaaatggttg gagggcacaa gttctacgat cgtaaagaaa tcaaagatat
     3001 tttagcgtat ttgaatgtgc tggtaaatcc tcaagattca atcagttttg agcgtatcgt
     3061 taattcgccc aaacgaggca tcggaccagg atctatcgaa aaactacgca gttttgcttc
     3121 tcttcatgaa tggccactgc ttgaagcagc gcaaaatgtg gatctggcaa atatcggtgg
     3181 aaaagcagga cagcaattag gtgcatttgg agagatgatc caagaagtca ctcagatgat
     3241 cccatacttg acagtgacag aattgacgaa agaggttttg gatcgtagcg gatatctaga
     3301 agacttaaaa atccaaaata cattagaagc acaagcacgc atcgaaaact tggaggaatt
     3361 tttaacggtc acacaagaat tcgacaaaca attcgaacag caaaatgaag aagacgctga
     3421 tgcaccagaa gaaaaactaa cggtcttctt aaatgatctg gcacttgttt ccgacattga
     3481 caatttagaa gaagatgctt cccaagtgac attgatgact ttgcatgcag ccaaaggatt
     3541 agaatttcca gttgtattcc tgattggtct agaagagggg gtttttcctt tatctcgagc
     3601 attgatggaa gaaagcgaac tggaggaaga acgccgtctc gcatatgtgg ggatcacgcg
     3661 agcagaagaa gcgttgtatt taacaaatgc tttctccaga acattatatg gacggacgca
     3721 atataaccga ccttctcgtt ttgtagaaga aatcgatcag gagttactgg aaatcgaagg
     3781 catgcgtcct acgcctaaaa aaacgcctgt attcgctaaa aaaacagcat atagctataa
     3841 acagccagag acagcggttg ttccctctaa aagtgctact ggaggggaaa ataacagctg
     3901 gaaacctgga gataaagtca aacacaaaaa atggggtcta ggaacagtcg tccgagtaag
     3961 cgggacgtcg aaggatctag aactagatgt ggctttccca agtcaaggag taaaacgtct
     4021 tttggctgcc tttgcgccaa ttgaaaaagc ttagtagtgg agggtaaatg atggaacatg
     4081 aaatgacgat tgacgaagct gctaaacgag cagaagaact tcgtacaaga ctgaaccaat
     4141 ggtctcgcga atactacgta gaagataaac caacagtgga agactatgtt tatgataaag
     4201 aatacgcaga attagtagca atcgaggaac aatatcctga tttgatcacg tctgattctc
     4261 ccactcaacg agtaggcgga aaagtattag aaggttttga aaaagtcact catgatattc
     4321 cgctttacag tttgaacgac gttttcagca aagaagagct gatagctttt gatcaacgtg
     4381 tacaaaaagc agtgggacgt gtagttgact attgctgtga actaaaaata gatggacttt
     4441 ccgtttcttt gcgttatgaa gatggaaatt tcgtgagagg tgcgactcgc ggggatggaa
     4501 ctgtaggaga aaacatcact gaaaacctaa aaactgtccg gtcggtccca atcaaattaa
     4561 aagaaccaat gaacatcgaa gtaagaggcg aatgtttcat gccaaaacgg tcatttgtcc
     4621 aattgaacca agacagagaa gcagagggaa aagatatctt tgcgaatcct cggaacgccg
     4681 cagctggttc tttgcgtcag ttagattcta aaatcacagc gaaaagaaat ctggacactt
     4741 ttttatacac agtagctgat ttcggtccta tgcaagcaaa aacccagtat gatgcgctag
     4801 aagagctaga aaaaattgga ttccacacga atcgggaaaa acgtctatgt cattcgattg
     4861 atgaagtgtg gtcatacatt gaagaatatc atgacaaacg tgtggatttg ccctatgaaa
     4921 tcgatggaat cgtcatcaaa gtgaatgaat tctcgctaca agatcaactt ggttttacta
     4981 tcaaagcacc gagatgggca gcagcttaca agtttccgcc agaagaagtt gaaacgctaa
     5041 tcgaaaacat cgaatggaca gtcggtcgaa caggggtagt aacgccaaca gctatcatga
     5101 caccagtccg agtagcagga acgacagtaa gccgtgccag cctgcataac ggtgattata
     5161 ttaagttgaa ggatatccgc ttgaaagata cagttctgat ttataaagcg ggagacatca
     5221 tcccagaagt ttcgcaagtc gtgttagata aacgacctaa agacagcgaa gaataccaat
     5281 tgcctacaca ttgtccagtt tgtggaagtg aacttgtcca tttggatgaa gaagtagcct
     5341 tacggtgcat caatcctaaa tgccctgctc aaatgaaaga aggtttgaac cactttgttt
     5401 caagaaatgc gatgaatatc gatgggttgg ggccgcgcgt actagaacag atgtatgata
     5461 aaaaactagt agcagatgtt gctgatcttt acaaattgac agaagaagag ttattgacgt
     5521 tggacaaaat aaaagaaaaa tcagcaaata atattttgac agcaatcgac aacagcaaag
     5581 ataattcggt cgagcggttg attttcgggt taggaatccg acatgtcgga gcgaaagcag
     5641 caaaaatttt agcagaacat ttcggtgatc tagaaacatt gagtaaaagt gactatgagt
     5701 cgattattgc gcttgatacg atcggtgata tcattgccga cagtgtagtc acttatttca
     5761 gtaacgaaga agtccatgaa ttgatgaatg aattaaaaca agcaggcgtc aatttcgaat
     5821 acaaagggct tcgaaatgcc caattgcaag aagtcgagtc tccttttaaa gaaaaaacag
     5881 tagtccttac tggtaaacta acacgattta ctcgtgaaga agcaaaagag acgatcgaaa
     5941 atcttggcgg gaaagtgact ggtagcgtat cgaaaaaaac ggatatcgtg gtagcaggag
     6001 aagatgctgg cagcaagctg accaaagcac aagaattagg aatcgaagtt tggacagaag
     6061 atcaaatggc agatgcattg gctaaaagtc gctcagtaga agaatgaaaa atgagttttt
     6121 tcagacaaat ttcaataaac aatttcagac acggcactct tgtgttaaaa tagagagcaa
     6181 agtttttaac tcgtttagaa agaaggggta gtatggcgat tagtgaagaa caagtgaaac
     6241 acgttgccaa attagcaaaa cttgcgttcg cagatgaaga acttgcaagt tttaccgacc
     6301 aattaggtaa aatcatcgat atggttgaaa tgctggaaga agtagatacg gaaggtgttc
     6361 catttacgtc gaatgtggtt gaaacagtaa acgtgatgcg acaagatcgg gcgcaagctg
     6421 gatggaaccg cgatgaatta ttgaaaaatg tgccggaaac agaagacggc tttatcaaag
     6481 tacccgcaat catcgataat ggggaggctg gcgcataatg acagaattac acaatcgttc
     6541 tctagaagaa ctgcatcata tgcttgtatc gaaagaaatc actgtacaag atttgacaaa
     6601 aacaacattt gaacgaatca aagaaacaga gcctgaggtc gaggcattca ttacattgaa
     6661 cgaagaaaaa gcattggaac aggcaaaagc tttagatgaa aaagggattg atgaaagcaa
     6721 cgtccttgct ggtattccaa ttggtatcaa ggataatatc gtaacaaaag atatcttgac
     6781 aaccgctgca tctaagatcc tttacaattt tgaaccaatt tatgatgcaa cggtcatgga
     6841 taaaatctat caagcagata tgatccctgt tggtaagcta aacatggatg aatttgctat
     6901 gggtggaagt acagaaactt cttatttcaa gaaaacaaaa aatgcttggg acccaacaaa
     6961 agtacctggt ggctcttctg gtggttctgc agcagcagtt gcagctggac aaattccagt
     7021 ttctttaggt agtgatactg ggggaagtat ccgccaacca gcagctttta acggcatcgt
     7081 agggatgaaa cctacttatg gccgtgtctc acgttttggt ttgattgcct ttgcttcatc
     7141 tcttgaccaa atcggaccaa tgacccgtac ggtgaaagat aatgcattag cattgacagc
     7201 tatcagcggt tatgacgaaa aagacggtac ttcttcaggc gtttcagttc ctgattttgc
     7261 agagggactg acaggcgaca tcaaaggaat gaagattgct ttacctaaag aatacttagg
     7321 cgaaggtgtg gcccctggcg taaaagaagc tgtattgaaa gcagcagaaa cattccgttc
     7381 attaggtgct gttgtggaag aagtcagctt gcctcattca aaatacggtg tggctgttta
     7441 ttatatcatt gcctcatcag aagcaagttc caatttgcaa cgattcgatg gtatccgcta
     7501 cggttatcgc tctgaaaatg tgaaatcatt agatgatgta tatgtcaact cacgttcaga
     7561 aggattcggt gaagaagtaa aacgccgtat catgttaggt acattctcct taagcgcggg
     7621 ttattatgac gcttacttca aaaaagcagg acaagtccgt acattgatca aacgagattt
     7681 tgaaaaagtc tttgcagatt atgacttgat catcgggcca tcttcaccaa cagttgcatt
     7741 cggaatcggt gagaacatta acgatccaat cacaatgtac atgagtgaca tcttaacgat
     7801 ccctgtcaac ttggctggac ttccaggtat gtctgtaccg gcaggtttat cagaaggatt
     7861 accagttgga ttgcagatca tcggtaatta ttttgatgaa aaaacgatgt atcaagcggc
     7921 atatgcgttt gaacaagcaa cagaattcca taaacaacaa ccagctattt taggagggaa
     7981 agacgaatga attttgaaac agtcattgga ctagaagtcc acgtcgagtt aaaaaccaac
     8041 tcaaaaattt tctctccagc gccagctcat tttggtgcag aaccaaacag caatacaaac
     8101 gtgatcgact ggggttatcc aggcgtgctg ccagtaatga ataaagctgc gctggaattc
     8161 gggatgaaag cagctttggc attaaactgt gaaatctcta aagatactca ttttgaccgg
     8221 aaaaattact tttacccaga taatccaaaa gcatatcaaa tttcccaatt tgatcaacca
     8281 atcggtcatg acggatggat cgaaatcgaa gtagaaggta aaaagaaaaa aatccggatc
     8341 gaacgtgtcc atttagaaga agatgctgga aaaaacatgc atggtattgg cggttattct
     8401 tacgttgatt tgaaccgaca aggcacgcct ttgatcgaaa tcgtttctga agctgatatg
     8461 cgttcaccag aagaagcata cgcttatcta gaagcattgc gatcaattat ccaatttaca
     8521 gaagtcagcg atgtgaaaat ggaagaaggc tctatgcgct gtgatgccaa tatttctctt
     8581 cgcccttatg gacaagaaga gttcggtacg aaagcagaat tgaaaaactt gaactcttta
     8641 tctttcgtga aaaaaggttt agcttttgaa gaaaaacgtc aagcaaaagt tcttctttca
     8701 ggaggagaaa tccaacaaga aacacgccgt tttgacgaaa caacgaataa aacagttttg
     8761 atgcgtgtca aagaagggtc tagtgattat cgttacttcc cagagcctga tatcccgaaa
     8821 ttcgtgatcg atgacgaatg gattcaaaaa gtaaaagcta gtttgcctga gatgccagct
     8881 tctcgtcgcg aacgctatat ccgtgagttt ggacttccag agtatgacgc aatggtattg
     8941 acacttacaa aagaaatgtc tgattttttt gaagctgttt tggaaaatgg tgcagatgcc
     9001 aagcaagcat ctaactggtt gatgggtgaa gtttctgctt acttgaacag tgagaaactg
     9061 gaattagctg agacaaaatt gacacctgag aacttagcag gaatgattcg tttgatcgcc
     9121 gatggaacga tcagctcaaa aatcgccaaa aaagtcttcc gtgaattgat cacaaatggc
     9181 ggagaagccc aagaagtagt tgaaaaaaat ggttgggtgc aattatctga tccgaacaaa
     9241 ctactgccaa tcattaatga aattttggat aacaaccaac aatctgtcga tgatttcaaa
     9301 aatggaaaag atcgtgcggt tggcttccta gtcggtcaaa tcatgaaagc aactaaagga
     9361 caagcaaatc ctggtgtcgt gaataagcta cttaaagatg aattagcgaa acgatagagg
     9421 ctgaaaatga aaaaagcacg cgtaatttat aaccccacct cgggaaaaga attattgaaa
     9481 aaaaatttgg cggatatcct acaggcatta gaagaagcag gatttgaagc cagcgcctat
     9541 gcaactacac cagaaccaga ttcagccaag aatgaagcat gtagagtcgc agaactaggt
     9601 tttgatctag tagtggcagc tggtggcgat ggcacaatca acgaagtggt taatggaatc
     9661 gctccgcttg aacaccgtcc taaaatggcc atcatcccag ctggtacaac gaacgactat
     9721 gctcgagcat taaaaattcc tagagataat attaaagcag cagcagaagt gataaaaaaa
     9781 caccaaaccg taaaaatgga catcggcctt tctgataaca gttacttcat caatatcgct
     9841 gcaggtggct atttgacaga attgacttat gaagttccat cagaactaaa gagtattttt
     9901 ggttatcttg cttatcttgc gaaaggagca gagatgctcc ctcgagtaaa gccaatcaaa
     9961 atgcgcttga agtatgatga aggggaatat atcggaaatg cttcgatgtt tttcctaggg
    10021 ctgacgaact ctgttggagg attcgaacag atcgcgccag atgccaaatt ggatgatggg
    10081 aagttttctt taatcattgt gaagacagca aatatctttg agatcttgca tattgcagca
    10141 ctcatgctga atggcggtcg acatgtcgat gatccaagaa taatctatac caaaacaagc
    10201 agtttgttcg ttgaaacaga agatgatatc aatcgaccag taatgatcaa cttagatggt
    10261 gaatacggcg gggaagcacc gatgcatttc caaaatcttc atcaacacat tgagttcttt
    10321 gccaataccg atcagattcc tgatgaagca atcacaggta cagatgaaga agaattagag
    10381 gttgtcagca aagaattcgt aaaagaagtc gaacgtttaa cggatgaaga tatcgacggt
    10441 gacggaaaaa tagcagacga aacacctaat caaaagaatt aatcaaaagc cggtgctgaa
    10501 cgtagtaagg tcagtgccgg ttttagttgt gtaattagct gatttatagt agagttcagt
    10561 aggattgaaa aataggctta aaggagcaaa atatgacaga acagcaaata gtaaagaaaa
    10621 acgaacgata tacagtcgaa atcgtcgact tgagttatga aggaatgggg gtagcaaaaa
    10681 tcgatggtta tccattattt atcgaaaatg cactccctgg agaagaagta gaaatactag
    10741 tagtaaaagc aggcaataag tttggatatg gaaaagtcga aacatttatt acacaatcac
    10801 ctgatcgtca gcctgtagaa aacaatgcat tacttcgcat aggtatcgca cctttagccc
    10861 atttgaagta tgaacaacag cttgtcttca aacaaaaaca agtagagcat gtaatggcga
    10921 agattgctaa gatgccagaa gttcctgtac ttccaacggt cggaatggca gaaccattcg
    10981 gctaccgtaa taaagcacag atccctgttc gccgtatcga tggacagtta gccaccggtt
    11041 tttacaaaaa gaatagccat gatctagtcc ctatcgaaga tttttatatc caagatcctg
    11101 tcatcgatca agcaatcaaa gtagtcagag atatcttgca acggttccaa gtacgaggat
    11161 ataatgaaga aaaaaatgaa ggacagcttc gtcacatcat tatccgtcgt ggtcattatt
    11221 cccatgaaat gatggtcgtt ttagttactc gtaaagaaaa attctttaaa tccaaggaaa
    11281 ttgctgaagc tattcatgaa gaattgccag aagtggtttc ggtcatccaa aatatcaatg
    11341 aagaaaaaac aaatgtgatt ttaggagaca aagaaaaagt tttatatggc agagactata
    11401 tcgaagatca gctattagga aaaacctatc gaatctcagc aaagtcattt tatcaagtaa
    11461 atactcaaca agcagagtat ctgtaccaaa cagccatcga gtttgccgag ttagataaaa
    11521 aagacattgt gatcgacgct tattcaggaa taggtacgat tggcttgtct ttagcagaca
    11581 gagtggataa ggtgtatggg atggaagtta ttccagaagc aattgaagat gcacagttca
    11641 atgctttaaa taataaaatc gaaaatgttc attatgaagt cggaaaagca gaaaaagtta
    11701 tgaaaaaatg gcaagaaaaa gggatcaaac catctgtgat cgtagttgac cctccacgaa
    11761 aaggattaga tcatcatttt attgaatcag caatcgaaat gtcaccagaa cgaatcgtct
    11821 atatctcatg taatccggca acttttgcaa gagatgctaa gttatttgca gaagcaggtt
    11881 atgccgtgaa gaaagttcag cctgtagatc ttttcccgca aacccatcat atcgaattag
    11941 tcgcttcatt cgttaaagga gaataaaaat ggaccaaaaa gaaaaaatga aagaattgat
    12001 caaaaagaaa caaggtggag gccgatccaa acaaatggaa acacctaaga atgatcgtaa
    12061 aaacatgaga aaaggcccta aaatctttaa taaatagacg tgctaaagaa agactaagaa
    12121 gtgttaactt ttgttattgt ttcttagtct ttttagttag tctacattat tttggcgatt
    12181 tgccaataag gactgttgag ttgtgcaaaa tcaaatctgg gtcagaatga acgtcattct
    12241 gtctcagatt tttcgttatt tcaatttgat taactgattg atcaaaaaga tttttatttt
    12301 catattctca aatgattcaa cactaaatat acatgtttga cgcggaaatt attattcgat
    12361 tttggcggca tcagggggga tgacgacgta gcagacccat tattttactc aagaaaatgt
    12421 ccgtgcctat tatttagaat acgatagtga acgtgctggg agttttgttc ttttagagaa
    12481 agtctctgaa gataaattag ttgtcttaga ttttgactgt aacgaaaaat ggcgaaatgg
    12541 aagagcacca gcaagtgatt gaccggatct acgaagcgac acgatatctg cctttggatc
    12601 gtttgtgctt aagtcctcaa tgtggatttg cttcaacaga agaagaaaat gttttgtttg
    12661 aagaacaatg gaaaaaattt cttttgtcaa aagcattgca gaagaagttt ggggaaaatc
    12721 attaaatcat ctaggagagc ttgataggaa ataacactat ggtataatca gaatgcttaa
    12781 tactaactac tgagggagcc ttatgaaaaa gaaaattctt aaatttatcg gatggttctt
    12841 cggaatcatt cttgggatcg cgtttgcaat ctttcttgcc ttccagcttt cccccagacc
    12901 tggagcattc ttgatcaacc atatgttttc aaatactgtt caaatcactg ataaaaaaac
    12961 gttcgatcaa gcagaaaaaa acgtggcgaa aaaaacagat ttgacgtatg agtccaagta
    13021 taaagagaat acttatgaca tctactatcc aaaaaacagc aaggggcctg ttcctgttct
    13081 attttgggta catggtggag gattcgtcgg cggagataaa tcgggtgtca aagaatttgc
    13141 gacaaaatta gtttcagacg ccaacatcgc agtggttgcg atgaattatg aattagcgcc
    13201 tgattcagaa taccctaatc aagttcttca agtgaatgaa ctgatcaaag atttattagc
    13261 tgaagaaaaa gacgacaaat tgctagacat ggaacaatta ttttttggtg gagatagtgc
    13321 aggttcacaa atcgcattgc aatatgcagc tatacaaacg aatgatgcgt atgctaaaca
    13381 aatgaaaatc aaacagctac ttccaaaaga tagtctgaaa ggaaccattt cttattgcgg
    13441 acctgttgat ttgaagctaa cagcacagca gcattcggat gatcgattta tgaagttttt
    13501 cgtcaaaacc gttgcgtggt cgttgattgg aacaaaagat tggaaaacga gtgagcaatt
    13561 gaaagaagct agtgtggctg accatgtttc taaggaattt ccgccaacgt atataactga
    13621 cggaaatgca tactcattcc aggagcaagg tcaagcatta gaaaatcggt tgacagaatt
    13681 aaaggtaccc gtgcaatcct tgttttatac agatacaaaa aaagagatta cccatgaata
    13741 ccaatttgat tacacgctaa aagaatcgca aaattgttac cagcagacat tggattttgt
    13801 gaataagtat aagtaagagg ttgtgaaaaa gccgtttcgt tttgagtatg aagaaacgaa
    13861 gcggcttttc acaacctctc tttcaatacc tttttgttct tgggataaaa ctttccctaa
    13921 caatcaatcg tgcaggataa gtcaaaatga ttggttcgtc ccgtataccg tcaattcgct
    13981 ccaatgccat tcgaacagct gcttgcccaa ttgatttcgc attgatttga acactggtca
    14041 agccagggga gaggtttgcg attgtctcga tattatcaaa gcttgctaca acaatctctt
    14101 ctccaatttg gatatttttt tgtttcaatg cccgatagac accaattgcc attggatcgc
    14161 tcccgacaag aatggcagta ggaagagggg attcattttc taatagatta gtcgcaagta
    14221 cctcaccttg tttttctgtc cagccaccta gatgataaga catatatttt tctaagtgat
    14281 gatcgatcat aaaatgctga taggcacgca tcctcttttc atttatagaa atctgcttcc
    14341 caccgaattc atccatatcg actttatatc ccccgatata ggctattctt ttatgaccat
    14401 tttctaagaa caattccagt acggttttag tcatccgttc aagatctgca tacaccatat
    14461 cgacctcttg ctggatgtca gggttatcca cgacgactat ttgcatgttt tgttttaaca
    14521 aggcggtaat cgattctttc gtaacagtcc caacaacgat caccgcacct aactgatcta
    14581 gatcttccca ttgtttagga ttatcagata gattatagat tcgattcatc cctaaatgca
    14641 gacgtcgagc ttcttcttcc atccctactc gcaaatgttt gtaatagata tcctgttctt
    14701 cgctcgcttt gccgatcgta gagatcacac caatcgaagg cattctcttt ttgatatatt
    14761 tacgagtact gatgtcataa tccaacgcag cagctgcctc tactatccgt tgccgagtag
    14821 tctgagccac atggatcgtc tgatcgttat tcaacacacg ggaaactgta gcaggagaga
    14881 catttgcctt tcgtgcgaca tctctaattc caaccatttt tacacactcc tcaaaaaaat
    14941 aaaaaatttt tattgacaaa attgtttagt aaacagtata caggaattat aaaaaaaagt
    15001 aaacggtttc cgttttgttg ggagtgaaat gatggatatt acgcatagaa aaacagataa
    15061 aaatttcatc ttatttgatg aaaagagccg agtcttccat ttacgaaata catttctttc
    15121 ttatctgata aagatagaag aaagcaatgt attggcgcat atttattttg gcaaaccagt
    15181 caagcagtac aaagacaata aaaattatcc ccgaagagat cgtggatttt caggaaatgt
    15241 tcccttaaat cctgaccggt ctctgtcgaa agatacacta ccgcaagaat actcttctca
    15301 tggcagcatg gactttcgaa cgcccgcatc gatcattcag cgcaagaatg gatcagactt
    15361 actggatctg agatatgaca gccattatat tacagatggg aagccagaga tcgaaggctt
    15421 gcctcaaact tatgtgatgg acaagtcaga ggcacaaacg ttggtgattt cacttaagga
    15481 tagagaaaca gcgatttatt tcgatttgtt ctataccata tttactgata gagcagtgat
    15541 cacacgatct gtaaagataa gaaatgaaac cggagaaaca atcaaactag aaaaagcggc
    15601 tagtttccag ctagactttg cccatactag gcgatttgat gaagtcattg ctttgccagg
    15661 agcacatgtc aatgaaagac agatttcacg gcaatctgtt ttatctggaa caaaagtatt
    15721 tgaaagccgt cgaggaacga gcagtcacca tatgaataac tttattgctt tagttcatca
    15781 tcatacgaca gaaaatactg gagaagcgat cggacttcaa tttgtttatt ccggcaacca
    15841 ttctttcgaa cttgaaaaag atcagatcaa tcaattgaga gtcgtcggtg ggatcaacag
    15901 ccatcgtttt tcgtgggaat tgaatgcagg ccagtctttt caaacacctg aaatgatcct
    15961 ttcttactca agtcagggat tgaacaaaat gagtcagatc caccatgagt tgctacgtga
    16021 aagaatcgca agaggtcgtc atcaatttgc tgaacggccg attctggtca ataactggga
    16081 agccacttac tttgacttca atagtgaaaa aatcaaggcg atcattgatg aagcaaagga
    16141 gctaggcatc gaaatgttcg tattagatga tggctggttt ggaaaaagag atgcggacaa
    16201 ttcttcatta ggagactggt tcgagtatga aggcaagttg acaaatggat tacgtgaaat
    16261 agcagattat gctcatagca aaggattgaa atttggttta tggtttgaac ctgaaatgat
    16321 ctcggttgat tctgaactgt atcgaacaca tcctgatttt cttatgcagg agccaggtag
    16381 aatgccttct gcttccagat cacaacatgt actggatttt acaaggttag atgttcggca
    16441 gacgattgag aaacaaatga gaaagatttt agatacgatt cctcttgact acatcaaatg
    16501 ggatatgaac cgttcgcttt cagatgtcta ttcgattacc cttgacccac agagacaagg
    16561 agaagtagcg catcgttata tgctcgggct ttatgaacta cttgaacatt tgacaactga
    16621 ttatccggaa atcttatggg aaggctgttc tggtggaggt ggacggtttg atgccgggtt
    16681 tatttattat atgccgcagt catggacaag tgataacacg gatgcagtgg agcgtatgaa
    16741 aatccaatat ggaacaagcc ttgcttatcc gatttcgtcc atcacagctc atgtatcagc
    16801 tgtaccaaat catcagaccg acagatctac ttcgttgaaa acaagaggag aaacagctat
    16861 gagcgctgtt tttggttatg aactggattt gacaaagctg tctcctgaag aagaaaagca
    16921 agtaaaagac caaattatat cctatcaaac gattcgtccg gtgatccagt atggccatta
    16981 ttatcgtttg gcgagtcctt ttgaagaaaa tattgccgca tggatgtttg tgtctcctaa
    17041 acaagatgaa gctattgtgt ttttagggag gatactagct tcagctcaac cagcgttcca
    17101 tgaagtatat ctgatggggt tagatgatga ggcactttat caggaacaga cctcgaagcg
    17161 gatattttcg ggggccgaat tgatgacagt tggactttac ttccccgatt ttcaaggtga
    17221 tttccaaaca gaactgcttc atttcaaaaa gttatgagag agaaggaaaa aagtatgaaa
    17281 gcaaatataa aatggttaga tgatccacaa gtttttagag tgaaccaatc aaatgcacat
    17341 agcgatcatg tttgttatgc aacaagggaa gaagtagaaa ccacatcctc ccttaaacag
    17401 tctttaaatg gatcatggcg tttttgttat tcgccaaatc cttcggtacg accaaaaaca
    17461 ttttataaaa aagattttga tgcctgttct tttgatgaaa tcactgttcc tagtcatatt
    17521 gaattagcaa attatgacaa gatccattac atcaatacga tgtatccgtg ggagggacat
    17581 gtatttcgcc gtcccccttt cacgacaagc cctgagaaag aggcggaagg gatcttttca
    17641 gctgctaaag acaatccggt tggttcgtat ttgacgactt ttcgattaga agaaggtcta
    17701 aagggaaaac aaatctcgat tcattttgct ggtgtagaac aagcaatgtt tatttggttg
    17761 aatggtcatt ttatcgggta tgccgaagat tcgttcactc cgtctgaatt tgatttaaca
    17821 ccttatttgg aagaagaaaa tattctagct gtggaggttt acaaaagaag tacagctgca
    17881 ttcttggaag atcaggactt tttccgattt tttgggatct tcagagatgt cttcttagtt
    17941 gctcgaggac aaaaccattt agaagatctt tggatcaaac ctcgcttaga tgacaaatgt
    18001 caaaatggca aacttgaagt agatttgtct tttgagcaag tagccaaaaa cagtcagctg
    18061 aatgtagcga tactagatcc tgatgggaag gaaatctatc aacagcaata tccgttaaaa
    18121 gagcaaatga cgattcgcat cgatggattt gaaaatgtgg cactgtgggg gcattttcag
    18181 cccaatttat ataagcttga attggagacg atagacgaag aaaaaagagt aacggagtat
    18241 attgactatt ccttcggttt tcgtaaaatc gaaatcaaag ataaaatcat ctacttcaat
    18301 ggaaaacgga tgattatcaa cggggtaaat cgacatgagt ggcatccgaa aaaaggacgc
    18361 gccatcacta tagaagatat ggaaaaagat ttggctatca taaaaaatgc aaagatcaac
    18421 ggcgtacgga catctcatta ccccaaccag atcacttggt attatttatg cgaccaagca
    18481 ggaatccatg tgatggcgga aacgaacctc gaatcccatg gctcatggca aaaaatgggc
    18541 gcatttgagc cttcttacaa cgtaccaggt gcagtacctc agtggcaggc ggctgttctt
    18601 gatcgagcaa aaacgaattt cgaaacatta aaaaaccatc caagtattct tttttggtct
    18661 cttggaaatg aatcttatgc ggaagaaaac attcgacaaa tgaacgatta cttcaaaaaa
    18721 gtagaccccg atcgacttgt ccattatgaa gggaattttc caaatccagc attcgaagat
    18781 tcgatttcag atgtaaaaag ctgcatgtat gcgccaccag aagagatcat tgactacctt
    18841 gaaaataaag cggaaaaacc gttcatctta tgtgaatata tgcatgacat ggggaattcg
    18901 ttaggtggaa tggattctta catgacgttg cttgatcgtt atgaaatgta ccaaggaggc
    18961 tatatatggg attttaaaga tcaggcgatc caagtgactg atccaatcac aggcagagaa
    19021 gtgatgcgct atggcggtga ctttgacgat aaaccaagtg actatgaatt ctcagggaat
    19081 gggatcgttt ttgcagatgg acaagaaaaa cccgccatgc aggaggtaag atattattat
    19141 gaaaaataca gtaaataaaa gtcatatgga tacggaaaaa gttgcaatcg tcttcggcga
    19201 ctgtacatta ggtgtcaaat cggggaatac gcattatatt ttttcttata caagaggcgg
    19261 actggaatcg ctcaataaaa acggaaaaga atggctatac cgggaaacac ttccagtttt
    19321 ttggcgagca acgaccgata atgacagagg tagcggattt ggatttaaat ccggtatgtg
    19381 gttaggagcc ggattgtatc caaaagtcaa agaaatagaa gtactggtgg acaataaaaa
    19441 gatcgatttg ccgattgctc cagtaaataa tcaatattca agtaatgaat acgctgaaac
    19501 ggttgaaatc atttttacac tggaaatcaa cacagtacca aaaacccaag tgacaatttc
    19561 ctaccaaatc gctggagagg gtaaaatgct tgtcaatatg cattattatg gacaaaaagg
    19621 attaccggaa ttacccgcat tgggtttaag gttcattatg ccaacgaaag caaaattttt
    19681 tgagtatgaa ggcctatcag gggaaacata tccggatcgg atggctggtg gtgtcgaagg
    19741 tatttataaa gtggcaggac tccccgtttc cccatacctt gttccccaag aatgcggcat
    19801 gcatatggaa acgaaatggg tagaactaac aagaaatcaa acgttggata atacggataa
    19861 agatcaagaa gatttttcgt tgcgatttga acagcaggat acgccatttg ctttttccgc
    19921 tttaccattc aaagcagaag aactggaaca ggcaacacat atagaagaac taccgccaga
    19981 aagacgaacg gttcttacga tttttgcaaa agtaagagga gtcgggggta tcaatagctg
    20041 gggagcggat gttgagtcac cttaccgaat cagcagcgaa gaggaccatg aaatcggctt
    20101 tattattcag tagcaatctt tagattatta tttgaaaaac ggagtttaca agaca
//
LOCUS       DAZKCC010000052        19759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000052 DAZKCC010000000
VERSION     DAZKCC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.053"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..1147
                     /locus_tag="ACG8VH_001904"
     CDS             104..1147
                     /locus_tag="ACG8VH_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9110456.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1320..1544
                     /locus_tag="ACG8VH_001905"
     CDS             1320..1544
                     /locus_tag="ACG8VH_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110457.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1547..1804
                     /locus_tag="ACG8VH_001906"
     CDS             1547..1804
                     /locus_tag="ACG8VH_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110458.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            1906..2259
                     /locus_tag="ACG8VH_001907"
     CDS             1906..2259
                     /locus_tag="ACG8VH_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110459.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            2312..3106
                     /locus_tag="ACG8VH_001908"
     CDS             2312..3106
                     /locus_tag="ACG8VH_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110460.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3135..4253
                     /locus_tag="ACG8VH_001909"
     CDS             3135..4253
                     /locus_tag="ACG8VH_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110461.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4286..4606
                     /locus_tag="ACG8VH_001910"
     CDS             4286..4606
                     /locus_tag="ACG8VH_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110462.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            4861..5550
                     /locus_tag="ACG8VH_001911"
     CDS             4861..5550
                     /locus_tag="ACG8VH_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFP9110463.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            5587..5754
                     /locus_tag="ACG8VH_001912"
     CDS             5587..5754
                     /locus_tag="ACG8VH_001912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110464.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            5774..7402
                     /locus_tag="ACG8VH_001913"
     CDS             5774..7402
                     /locus_tag="ACG8VH_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110465.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            7453..7824
                     /locus_tag="ACG8VH_001914"
     CDS             7453..7824
                     /locus_tag="ACG8VH_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110466.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            7909..8109
                     /locus_tag="ACG8VH_001915"
     CDS             7909..8109
                     /locus_tag="ACG8VH_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110467.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8243..9469
                     /locus_tag="ACG8VH_001916"
     CDS             8243..9469
                     /locus_tag="ACG8VH_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110468.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10066..10461
                     /locus_tag="ACG8VH_001917"
                     /pseudo
     CDS             10066..10461
                     /locus_tag="ACG8VH_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            10539..11578
                     /locus_tag="ACG8VH_001918"
     CDS             join(10539..11036,11039..11578)
                     /locus_tag="ACG8VH_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFP9110469.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            11591..12529
                     /locus_tag="ACG8VH_001919"
                     /pseudo
     CDS             11591..12529
                     /locus_tag="ACG8VH_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            12522..12869
                     /locus_tag="ACG8VH_001920"
     CDS             12522..12869
                     /locus_tag="ACG8VH_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110470.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            12866..13048
                     /locus_tag="ACG8VH_001921"
     CDS             12866..13048
                     /locus_tag="ACG8VH_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110471.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            13184..14041
                     /locus_tag="ACG8VH_001922"
     CDS             13184..14041
                     /locus_tag="ACG8VH_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9110472.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            14061..14621
                     /locus_tag="ACG8VH_001923"
     CDS             14061..14621
                     /locus_tag="ACG8VH_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFP9110473.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            15510..16277
                     /locus_tag="ACG8VH_001924"
     CDS             15510..16277
                     /locus_tag="ACG8VH_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFP9110474.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            16303..16848
                     /locus_tag="ACG8VH_001925"
     CDS             16303..16848
                     /locus_tag="ACG8VH_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110475.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            16849..17415
                     /locus_tag="ACG8VH_001926"
     CDS             16849..17415
                     /locus_tag="ACG8VH_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110476.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            17430..17678
                     /locus_tag="ACG8VH_001927"
     CDS             17430..17678
                     /locus_tag="ACG8VH_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110477.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            17697..17936
                     /locus_tag="ACG8VH_001928"
     CDS             17697..17936
                     /locus_tag="ACG8VH_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110478.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            17953..18537
                     /locus_tag="ACG8VH_001929"
     CDS             17953..18537
                     /locus_tag="ACG8VH_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110479.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            18644..18925
                     /locus_tag="ACG8VH_001930"
     CDS             18644..18925
                     /locus_tag="ACG8VH_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP9110480.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            18915..19256
                     /locus_tag="ACG8VH_001931"
     CDS             18915..19256
                     /locus_tag="ACG8VH_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9110481.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         7490 a         2614 c         4036 g         5619 t
ORIGIN      
        1 caacaacgat tatgatctta aaacaatcgt ttatgtaatt ggcaatctta cgaataattt
       61 tcatgcaata atgacaataa aaaatgaaaa ggggtaaaat caaatgaaaa aatttttagt
      121 aggaattcta ttcacaatgg ggctagtatt attatcacct tcagtttcag cagaaagctc
      181 tatttccatg tatcgtatgt ataatcccaa tagcggagag catctataca ctagaagcgt
      241 tggagaaaga gacaatctga aacgttatgg atggcattac gaaggtgtcg catggcaagc
      301 tccaacaagt ggtactcctg tttatcgttt gtataatcgg tataacggtg aacattttta
      361 cacagttgat gccggagaaa gagataagat ttcacacaca ggatggacgt acgaaggtgt
      421 tgcgttctat tcctacaacg gagcaaatag agttcctgta acacgtttat ataacaaata
      481 cgtgaattgg catcactaca cgttagatca aaatgaaaag aacgtaatta gtaaacaagg
      541 ttggaagatt gaaggtacag gctggtatgc aatgccaaac agcgtaccaa atcctacacc
      601 tgctccaagc actgaaaaag tgttgaacgt tcctgttgta tggcaaggaa atacaatgtt
      661 atgcgaagga gcttctttat tagaaggttt acactacaaa ggtgtagcca caaatcaaag
      721 cttaaccagt tttgtaaact atatgccaaa agctaatgac aataatcctt atcatggtta
      781 ctcaggagag tggcgtcata acgttaatgg aacgtatcaa ggcatgatgg ctgacccagt
      841 agttaaatgg gcaaaaaacg ttggcggtaa tgccgtcaat atcactggtt caggaacgaa
      901 tggaatcaag aatgaaattc gcaatggtaa tccggttgta acttggatta cttataacta
      961 tgctacgcca gagttcaaac aaatgccttg gggtcgtgca gtatggaatg gacatgtagt
     1021 tctcgtggat ggcttcagaa atggtgcata ccatgttgtt gaccctgtat ttggcgcaaa
     1081 atggatcaat gcaagcacgt atgagagagc atttaatgtg actggaatgg cggttgctgt
     1141 acgttaattg atagaattat caactaaatg aaataagagc ttaattactg aaatggtaaa
     1201 taggctctta tttttttaga ggaaattttt gtaatacgaa tctataacaa aaaatattga
     1261 atgtagtaca caaaagtggt atattaaaat cgtagagatt atgcgaagag gtgattttga
     1321 tgaaccaaag aagaagaaaa aggttagaag aaataagcga gcgactttcc gagctaaagg
     1381 atgagttaga agtaattcaa gaagaagagt acaaagaaaa tatacctaaa aatctacagg
     1441 gatcagaaag atatgaaaaa gcagaagaat ccgttgatca acttgaagaa gctgttgatg
     1501 gtttatccga catattagaa ttgattgaag atatagctag ctagagatga ttaatgaaga
     1561 tttcaggagt gagaataaaa tgattgatat ttcactgata gagaagttac ttttctcaaa
     1621 taagtacacc gcaaaagagc tggctacaag atggcaaata tccgaatcag ctattaaagc
     1681 ctatcgtcaa aataagggaa gcgtaggatg gcgtgattgg aaagggatca gcattggaaa
     1741 agcagaagac ataatgaaac gtgaaggact tcgatcctat accacttctg aaaaagacaa
     1801 ataacttaat gtttgaaaag ataaaatatc tgtcttaaag ctatgtcaaa ttcaaatggt
     1861 gaatgtttgc taaatataat gcaaaaaaag gaagggaaat attaaatgat aagcgaaaat
     1921 ttgtcttatg agaataataa acatctagtt aaagaaaata agaaagtaga agattataag
     1981 aacatgtata tagaaatgaa aaaatcacca ctgtgtattc aatataaaaa tgataattgg
     2041 aagcaagtag tcgaatttgt aaaacctcat agtatacgaa gagtacgaag ggggaagaaa
     2101 aaaagtgaac ttgtaaatag cggaagagcg gtaattagag ttataaccaa agattatcca
     2161 gttgaatatg gagattatat tttaaggtta agttatcatg cttttgttgt gttgaaagaa
     2221 gagcatttca acgagttttt caatttaaaa agtaagtaat catatttttt tgtagacgtg
     2281 tataaagtta actaaattgc gaggtgatga catggtagca aaaaagaata ctctaaaaga
     2341 agcttctgaa aaatatcaag atatgaaaag acgaggatta ataccgaaag acaaaaaagg
     2401 tatatggaga gaatttgtta aggaagtcag tagtgatcga agtggcggtg actttgttgg
     2461 acgatataca gcaaaaggct atagcaaacg attaaggatc actgttgaat atggaacgaa
     2521 tgaatcagct acagagctga agcaaaggta cttagataca cgaagggaag cttcaaaaaa
     2581 atataatgat ttcaagagca gaggtctgat tcctaaaaag aaaaagggaa tttggcgtga
     2641 gtttttcaag gaagaagata gcaatcggaa taagggagac ttcattggac gatacacagc
     2701 aaaaggctac actaagaatt tagggatcaa ggttgaatac ggaactggtg aaagttcatg
     2761 ggaattgaga aatcgctatt taaatacatt gttagagaaa tataaagcag aattattgga
     2821 gttgttcgat gttaatgatt ttatcgagat ggctgaaaag tatggtcatg aagaaggttt
     2881 tatgatcaat gacattatgg ttattgataa ttttacggat ttattagcac ttgcgaaaaa
     2941 agcaagggat ttaagagaaa cgcagatgta tctattcaga aataaaaacg gaagaaaccg
     3001 gagaactgaa tcgagtgaga ttgaagatta ctacgatgcc tttacacacg atctgggagc
     3061 ctttgtagac gagataagtg gtgtaatgaa caaatatagg gtctagaata aaaaagccgg
     3121 agatgagcag ataaatggga aacaatagca tatacacacc tgaaatgcag aataaaatta
     3181 aaagacttgt cggattaggg tggatgacaa gaggaaaatt agattctttt agtacgatca
     3241 aatatctttt gatggacatt taccgtcaga acaagcaaac aggaatacat tttttgggct
     3301 atgataagcc ggaagatgca cctttctttg ttccaagttc agcctataga gccagtacct
     3361 ccggtagcat gtataacttg gatttgtttg aggaaggagc aaagatgatc gaatatgatt
     3421 tcaacgaagc ctattcgagg atcatgagaa attatccttt gccgtcaaac acttatttga
     3481 aagcaaaata taatacagat caagctattt tgagagcgat aaatagacca aaagtaagac
     3541 cgtatgaaaa tttaagaacg tatatgttct ttttggttaa ttttgaagga acagccaaag
     3601 aaggaacata tgctaatcca aaatcacagt cgatgtttgc ggattatggc gaaaatccca
     3661 aaacagctaa tgagcctatt tggttatcgg agattgagct aacacttatc cttgattatt
     3721 atgatattaa agagatggaa gtcctagata gccacacatt tttgacgaaa aaaggattac
     3781 tcaaagaata ttttgaaaga atcgagccat taaaaactga tcctgatacg ttgaagcttt
     3841 ataaaaatct tagaacgaaa gtatttggtg ctatcggaca aaaatcatta agagaaggag
     3901 aaacaagcac cttcgataaa tacagtatgt ataatcgagc gtattcctcc atggtagcag
     3961 gcgtatttag agatcgtatg gttagatatg aacagaaata cgttcatagc gcatatgggc
     4021 tattaaaaat tcggactgat ggattgtatt tcagatccga agtaccagag tttgaagagc
     4081 tgtataaact tggagtagtt aagaaaaaag ttcatacaat atccaaagaa gaagtcgaaa
     4141 aagctttaga atttaaaaac aaaggatttg aaaataaggg ctcctttggg aaatctaaag
     4201 atgaagttaa gggtgttgtg atagacgatg atttcgagtt tgattttgac taaatactct
     4261 ttggaaaaga tggtaggagg taaatatgaa gaagaaaatt gtggaaggaa atgtaggtgt
     4321 tattattcta aaagattcaa ctgactatgc taaatcaatg gcactaaaag acgaaaaagt
     4381 tgcacgcatg gataaagatt tagttgaatt tctgatggat gatacagaaa gattaaaagg
     4441 ggatcagttt ttgagtttaa tgaaagacaa aggcttaaac acatatggtt tatttgatta
     4501 taagaaatta gctatagagt gggtaaaaga aggagaaata ttttttattg aagaatggga
     4561 aggtactgag aacttagttc ttcatcagta tcttttgaaa gcttaatcta agtaaaacag
     4621 ttctgactag ttatgcttgt cagaatttca aaggaagttt tccataacca tcatcattca
     4681 aaaataaagg atttgaaaat aagggttcct ttgggaaatc taaagatgaa gttaagggtg
     4741 ttgtgataga cgatgatttc aagtttgatt ttgactagat ttttttgaaa ggtttaagag
     4801 taaacaaggt aagaactaag atcatgaaga ttgagagtaa agaagataga ggacgaatgt
     4861 atgagaacac actatattta tttagaagat aaaattgtag atgaaattcc ggcaaaaagt
     4921 cgagaacaag ctagaaaatt atttattgaa aaatacaaag agatcaatgg agatagtgaa
     4981 gttccaaaag tggtcgtaac agttagaaaa aggcaaacaa aaaagagaag tacgtttcat
     5041 gtcagccctg atatatataa tcttatagag cgacatagaa gtgaagataa tatttctatc
     5101 tctcaatata ttgctacagt tattgatgaa ttgatttcag gaaagtatga ctatgctttt
     5161 gattttgagg gtgaaaagga ttacaaaagt gttgctgtct ttcaatctga ttttgagaag
     5221 ctaaaaaact atgctggcag gagaaatgtt aattttgcaa gactactaga agagttctac
     5281 tccgaagagt tgggagtagg aaatgaatac aagagagaaa cttatgcgaa attgtctcat
     5341 aagttatccc aagcgcttaa agaagtgaca gaagttaaag cggaactaga tgctaaactt
     5401 gaagaagtga aaaaatatca aacagagagg aagaacaaca tgaagaatca agtggaattt
     5461 gacgaacaga aagaatttga aaaagcaaga aaacgctata acaaaattat gaatgaactt
     5521 agctatgaac acttaacagt tcatgaataa ttgagtgagg gtacagaaaa atatacgatc
     5581 gcagatatgg ttaatgaagt ggaaactctt ttagcttctt tctttgaaaa tggtcacgta
     5641 aacgatgaat ggcgaaaaga agaacctaaa agggttaacg cttatattgc tcgattaaaa
     5701 aggtttattg ataaatataa ggagaatagt gacaaatttt ctaaagataa ataaggagag
     5761 agtgtgatta catttgccag aaatcgaagg aatattttcg ccaaaaatag gtaaaaaaca
     5821 gttagatttc agcagttttc cgagcgtaaa tagagattat aatctaggtg atgaggtaca
     5881 gatactaaat attgatttca aagaactaga cttcgctgtt gctgtttcca acaaacccaa
     5941 ggcattggaa cgagtgaaaa aaatatacca agagaatgat caaaatatca aaaatatgat
     6001 caaaaagtat agacgagata attcaaaagt tgaagacgtt ttattcaacg gtacggcaaa
     6061 acatgatttg aatgtttata tggcattagg agtggctttg tactgtaatt caattaatca
     6121 gacagatatt ttggttaata tcgtgaggaa tagctatcaa aaaatattaa aaataagtag
     6181 cgaaacaaac ccttattatg caatgaaata tcttggggat aatttattaa atgataaaaa
     6241 attgatgcac aacactgaag agctgttatc taatttggct gcgattaact ttctattcaa
     6301 aacaggttct gaagattatt ttatagataa cgtactaaaa ggtgcttacg gaacagcatt
     6361 ttatccgctt tacaatatga aacagcataa agccgaacaa aaggataaca aacttgaaat
     6421 taaaaagatg aaagaactat taggtatagg atttgtaaaa cccaattcaa aagtcactat
     6481 tgcttcgtta gcaactacta acaatgatgg gaaaatagta aatgggtttg acgattcaga
     6541 tatagatata ttgtcaaaat catatttttc tttgtatgga gattttgcag atttgatggg
     6601 tattgatagt tacttagtca ataattttga gatcaagggt agcgagctga atgatattgt
     6661 gtatttcgtt ttaatctctc tatatgcaat tgatcagaat accgagtacc gacaaattga
     6721 ctatttgcta gttttttata gtttgttata tcagttcgtt ttaactcggt tgtttaaaaa
     6781 tgctaaggaa gaattgatcc tgaattctga ttatgaaata gcaaaacaaa ttgcaagtat
     6841 tagaaaagaa aacaaatctg aagtaatcaa attaagaaaa gaattaagta ttgcgagtaa
     6901 agaaaacggt cggttagaat ctttagtcaa atcggcaaaa aaagaagagg aacttttgaa
     6961 gcaagagatt aaagaattaa aaggaatcag aaaagaactt gaacagctga ggtttgaaaa
     7021 taaagtatta aagcaattgc aggagcaaga acatagacaa tcattaaatg gtaaaactga
     7081 agagccttca ctgtctgaaa tgatagctta tctaaaacat aaaaacattc ttttagtggg
     7141 aggacatgta aattttcaaa ataaattaac tgaactatta ccaaatatta aaatgtttga
     7201 tgtaagcgaa aataatggaa agatacttaa aaatttgaat acgtgtgatg cgatttttct
     7261 atatacagat tacttgaatc atggtctata ttatcgtgtt atgagtgaaa tttctaataa
     7321 agataagata ttttatttgg ataatcatgt gaatatgaac ttaacaatta aacgtattta
     7381 cgatctttta tcacttatat aaagagagaa gtgataaata ggacacaaaa tttggaaagt
     7441 aggtaagtca ctatgagaga actaacttta tcttctaagg aattagaaaa gaaaactgct
     7501 ttattaaacg aaatatttag tgctgattgt ataatccaaa gtttaaaaga aagttcattt
     7561 tcaaaactaa cagatcagga aaaaatttct tatatgctaa gtttagataa tctaggaact
     7621 aaatatgatg aatcagcaag tagcgataag cttttgattg aattagtatc aagtcttagt
     7681 agttttggaa aaatgaaaag atttaatgtg atattagaaa tttttgcttg gttaatccct
     7741 tcatttattt ggttttttta tattttcata gttgaatctg agttaatagc caaggtctct
     7801 cttggactgt taattccgat ataaataggt tcagcaattt actttaataa cttgtataca
     7861 cctattcaaa taaaaaagca gttattacat gaaggattaa gtggtaatat gttaaaaaga
     7921 attgcatggg ataaagataa taaaacagat attgagctta aagaatataa atacaattat
     7981 ttctcgagat gtatagatag aaagttactg tttaaaaaag aaggattaga atttagtttt
     8041 gatgaagatg aatttgaaaa attagtgatg gactggcgta agaatttact ggatacggta
     8101 caattctaaa agcaattgag gtcgaaattc cgatacttga atcatgatct atattatcgt
     8161 gttatgagtg aaatttctaa taaagataag atatttcatt tggacagtca tgtgaatatg
     8221 agcttaacaa ttaaacgtat ttatgatctt ttatatgttt ataaacatta atgaggtgct
     8281 aaaattgaca acaataacta tgacaaaaaa aatagaagta tatgttgatg gatcacaaga
     8341 agaaagggat tccttttttg attttttttg gaaaaatagt agagaatatg taaaagcgtt
     8401 gaatctggta tataaacagc taatgtttga agagttgata aaagatgcaa ttaaagaaat
     8461 ggatcaaggt tatgctgaga gagaagaaag tattcaaaat cggattgaaa agttagcata
     8521 tactgatcca atagatcaaa aaaaacttga taaagcaaaa gctgaattat ctaaattgag
     8581 aaatgaaaaa aataaagaag cgagagaaat attaacgcaa gcgatagggt tgaagagtca
     8641 aacgagaaca cgagatgtag caaaaaaatt agactttgac tttggagatt ttgttgattc
     8701 cgcaaatatg aaagctagta aagattttgg caatgatttt atcggaattg tgaccggaca
     8761 aagaacgcct agaatatata aaaatagaaa caaatatgga ttattacctt ttagaggtcg
     8821 agatattaaa atccataaag atgataagtt ttatatttat ttacctaaag gctttaggtt
     8881 taaagtgaat ttaggtagca agccaagaaa agcaagagat gttgcttcag ttttacaaca
     8941 cattatggat ggcgattaca aattaaatca aagtaatctt atttttgatg gaagaaaatt
     9001 attttttaat ctaacaattt cttttgaaaa aagctataat gaaatggaat tcaagaaagg
     9061 taatgaattg atcgtaaact ttgcagattt gtgtagcggt gaatgtttaa ttaatgataa
     9121 atctgtaatg tcttttggtg atagccaata tataagaaac ttcaaaagta aaattttagc
     9181 aatgaaatca aaagaacaaa gccgaggagt cttttctaag ggcggtcatg gtcgaaaaag
     9241 aaaattagat caagatcgat gggaagcaat aaagaaaaga gaaagtaatt ttgttaaaac
     9301 ttataataat caatgttctg caaaaatcgt taaaactgct attcgcaata aagctcaaat
     9361 tattcgtatc attgaaccag ataaggctta tggcgaatgg gcttactatc aattgaaaga
     9421 acaactagta aacaaaggaa aaagatatgg gatagaagtc agaagttagt aaattaaaca
     9481 atcgttgatt tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt
     9541 caagttgtgt gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca
     9601 gcatgtttct caaattttta ggataaggga aattgctagc agtttgtttt agaaccatat
     9661 aaaacagcat gtttctcaaa tgagtacttt aaattaattg atggcgttcc tgctttagaa
     9721 ccatataaaa caacatggat ttaaaacaag aagtaagtca gttctccttt tactttagta
     9781 aggaaaaata aagtattctc aataaaactt aaaataagca aacctttgtt ttaaaatgat
     9841 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     9901 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     9961 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
    10021 aacgaacata cgtttgtata attacttgtg aatgggggaa taaaaatgtt tgattacagt
    10081 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
    10141 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
    10201 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
    10261 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
    10321 ccaccacgaa tgaacttata tataaaaaaa aatatggaag ttaatcagat ttttagaaag
    10381 tatgtcgcag atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca
    10441 aaatccttaa atttatttac tatataaatt gagttaaatt gtattcgttt ttcctcctat
    10501 aggcgtatac taaagaaaac acttgaggag tggggaacat ggaaacagaa cgccaaatcc
    10561 gtgaattgga aaaagcgatg aatacgacag acaaccgccg gttgtacgag cgtttcctgg
    10621 cagtcaagtt ggttcttgaa ggccacaccc ggaaagaagc cgggcacacc atcggccgcg
    10681 acgagcatac tgtcagccac tatgtgaaaa actactgcca taatggaatc caaggtttag
    10741 tctccaaaaa acaaagcgga cgaccttcac ggctgacaga tgaacaggaa gcggaactgg
    10801 tccaaatcgt cgcctaccac actccggagg aagtaggttt caaatcccgt gccaattgga
    10861 cattggcgat tgtgtgtgac ctgattctcc gggaatggag ttatggatat acgcaaaaag
    10921 gcgtggcgga cctccttcac cggctaggac taagctggac ccgtccgacg tataccctca
    10981 aaaaagcgga ttccaagaaa caacaggtat ttttggaaga aaccttcccg agcctaaaaa
    11041 aaaattgctg aatagcgata ttgaccacct cctattcgag gacgaaacca tgatccggga
    11101 ttaccaagcc attggctgca cctggtttct ccgcggccac cagcgaaaaa tccccactta
    11161 tggacaacac aaaggggtaa aattgatagg caccttggat tatgaaaccg gtcggatcat
    11221 ttgtacagaa gaaggggcat acgacgcagc ggccttcctc cgttttctgg agcggacgtt
    11281 gcgggaatac cccaccggaa aaatcgtgat ggtattggat aatgccctta tccaccacgc
    11341 caagctgatc cagtcttttc ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc
    11401 tccctacagc cctcagttga acctaatgga aggcgtttgg aagtggctca aggaatcggt
    11461 catcaacaat gttttttttg accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt
    11521 agccgatgta aacgagcgtc cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag
    11581 tatttaattc aacctatata gttcaggaac aagaagcgag agaagaagag aattagctaa
    11641 aataatcaaa gatgagattc agaaagaaac aggtctaata gcaacagtcg gaataggaga
    11701 taacccatta ttagctaaac ttgcactgga caatgaagcg aagagtagta aagaatttat
    11761 agctgagtgg acttatgaga ctgtacctga aaaaatatgg aaaataaacg agatgactga
    11821 cttttggggc attggatcac gcatgaaaaa aagattgaat catatgggaa ttatgagtat
    11881 agaagattta gcaaattgga atccctacac aattaaaagt cagttaggtg taatcggact
    11941 ccagttatat ttccatacca atggaattga tcgtacggat attgctattc caccagaacc
    12001 aaccaaagaa aagtcttatg ggaattcaca agttctacca aaagattata cgaggagaga
    12061 agaagtagaa ctagtagtca aggagatggc agagcaagta gccattcgaa ttagacaaca
    12121 caattgcaaa actggttgtg tacgcttagc aattggtacg tcaattttgg aagaaaaatc
    12181 aggtttctcg caccagatga aaataccaat tacagataat acgaaagaat tacaaaatta
    12241 ttgtcttttt ttattcaata agttttatga ggggcaggaa gtacgacata taggagtaac
    12301 ttatagcaga ttagtataca cagattccct acaattagat ttatttagtg atccaagaaa
    12361 aaagattgct gaagaaaatt tggataggat tattgataag attagacaaa aatatggatt
    12421 tacagctatt atacatgctt catctatgtt agaaggtgca agaagtatta cgagaagcac
    12481 tttaatagga ggacacgcag gaggattagg aggtttgcat aatgattgaa tataacgata
    12541 gaggacgttt aaaatggtcg ggattttatt tgtctgatca tactgaaaga atcaatgcag
    12601 acaatgtaca acgtgctaat cataatttgg caaaagaaca aatgacgact gaagagattg
    12661 cgaaaatact gagaactgct gtcataaaaa atagacccgt ctgcattcaa aaagaggaac
    12721 gaaacgctga aggttactat ccagatgatg tagtaggaaa aattattgga tatgacgagc
    12781 ttggtgtatt tgtggaagaa gaaaaagttc actatgatga aattaggaat gtttctttct
    12841 cggaattatt aaaatggagt cagatatgaa cattattaaa aaatacgaat tcggttatat
    12901 tacgtatgaa cagctaatag aggaattatg gggatatggt caacaattaa ttaatgaagt
    12961 ggggattgat tgcttttgtt tctatattga atccggagca ggataccata agaacagata
    13021 ctatatattt ccatatccac caaaatagtc tcttagatat ttttaaattt tctccaaata
    13081 ctttcaatta ttgtgaaata tttcccttta attaatcaaa ttttttataa aagtaatata
    13141 taaaataaat tgcttgaagt attatattta gatatcataa tttatggaag aaaaattaat
    13201 taaaaaggat gagattataa tggggttact ggttccgaga gctaatactt atactatacc
    13261 tttaacgaaa cgccaattaa ttgctaaaag atatcaaagg attactagag ccatcaatag
    13321 agagttttgg aattctgaaa gtgatactgc tcacagttta tacgtaggtt cttatgggag
    13381 aggaacggca attagtacta gtgatataga tataatagtt gaattaccga tggcagaatt
    13441 tgacagattc aaaaattatt tatcaaatgg cccgtctaag ttattacaag ttataaaaaa
    13501 cgcatttcag gaaatactac caaatagtga tattagagct gatggacaag ttgttaaaat
    13561 aaatttccat gatggaatca aatttgaaat agttccagct tttaatgaaa aagattattg
    13621 gggtgagtca aaagggttta tttatccaga ttcaaacatg ggagggaact ggaaagctac
    13681 aaatcccaaa aaagagcaag aagccatgaa attaaagaat acaaaaagta ataatttatt
    13741 atatgctacg tgtaaacatt ttcgacatgt acgagataca gaatttacga gttatcatct
    13801 ttcaggaata gtaattgaca gtttcgtata tgaggctatg ggaaattgga aattcgttga
    13861 aaataacagt gggggtcaaa acattagttc tgtatcttat gagacagctt tattagaata
    13921 ctataactca cataaagtta tgggcggttt aaatttatac tctcctggaa gcaatcaatt
    13981 tgttaattct gatagtagta tcatatgttt agaaaaagta ttaaaaaaaa tagctttata
    14041 aagttttatg gaggaacaac atgggagacg aaaaaaagcg cttggaagtt caattacgtg
    14101 aagcatatgg acgagtaacg tatacttaca ccactcattt aaagcaagcc gataatatta
    14161 tttctgacaa taaaatttta aggtgtcttc aaattggttt gtcagccctt actactggag
    14221 gggtacttag taccattatt actgataata gtgagttaac ctttgttgct ggattatgtt
    14281 caacaatttc tcttggatta aacttatact ttaagaattt tgatcttgct gaagaagcta
    14341 aaaatcatca aattgttgcc gataaattat ggttgatcag agaagaatac gtaagcttac
    14401 taacagattt aaatcgttta agtgttgaag atattgtgaa aaaaagagat caattaatga
    14461 acgaaactca tgaagcatat aaaaatgccc ctaaaacaaa tagtaaaagc tatgaatcag
    14521 ctcagaaagc attaaaatct gaagaagagc aattttttac agaagaggaa ttggataaga
    14581 tgttaccaga gcatcttaga aaaaagagtt ctaaatattg aactcttttt ttatgcttaa
    14641 aattctcttg taatttttct attcatctat atttaagaag aaatatgatg atagaaacat
    14701 ctcctaaagc caccgcaagc cgttctaagc atagcttagc ccgacattgc tagatatgct
    14761 catctcacca tgaacgtttc ctaaagccac cgcaagccgt cctagccatt gcctagtccg
    14821 acatcgctag attagtgcat ctcgccataa aagttttcta aagccaccgc aagccgttct
    14881 aagcatagct tagcccgaca ttgctagata tgctcatctc accatgaacg tttcctaaag
    14941 ccaccgcaaa ccgtcctagc catcgcctag tccgattttg cttgatcggt tccatttctg
    15001 tttatttagt gggtcaacac cgtaatctta aatagccatc gcctatttaa gattactagg
    15061 tctgctttga aggtaaaaag tctttcattc ttcaagactt ttaggctttc aagctacgcc
    15121 tgaaagcatt gaaattcatc gctctttatt tggttttgcc atttataatt ccttacgccc
    15181 attatctttt tcgcctgtca taccttgtca gaagtgttta gagcttatct catctttgga
    15241 ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt tacgaaaata atttacttaa
    15301 acacttgtat taatccggca gataagaacg cattctaatc actcccgaca aggcaagaca
    15361 ggcgaaaaag ataaataaat gaacgaaagg aagaaacata aaatgcaaaa ccaaatagcg
    15421 atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa aaaagaattt tgaaaaacca
    15481 aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag aattttagaa gttaatcaag
    15541 aaatggtaaa caagttaaca aatatcagtg ggaactatga aaagtacgat ggtactttac
    15601 ttgataacta tatttttcac gatacacaaa atatcagaat tggcagaagt aaaccgagag
    15661 cgttcattct catcaaagag aaatatttga atgaatggaa tagcacctat gaaatggtta
    15721 tgactgataa ctttgaaaag gtattggatt tcagaaatgc tttgttgggt tctgatatgg
    15781 gaaatagcag tcttgaatct attgaagttg aaaacaagac cgagaatgaa ggtgaaggat
    15841 tgattaagaa tgtcttgaaa atcgaacaaa aatatatcag tgaagataga agtttttata
    15901 gagtcacaag ctcggttggt ctagcaaact ttgctactaa cttaattgga aacaatgcta
    15961 gtgaatcttt gctagtgatg tgtctgaata caaagaatga ggtcattgct tatagcgaag
    16021 ttttcaaagg tggaattaat caatcagtag cgataccaag agagattttt caactagctt
    16081 tgctaaacaa ttcagcaaaa atcgcagtat cacataatca tccgagcgga tatgaatttc
    16141 caagtgaaaa tgacaaaagt tttactacga atttgaaaaa cgcaggaaaa acaattggta
    16201 ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac aagctattac tcatttcaag
    16261 aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca agatgggcga aagaacacaa
    16321 gcgttaatta cagtcaaata tggtaacagc aaaacgaaag caagcgtgtc gattcattat
    16381 cagtgggggt atggtcgata tatgctgatg gacgtattga atcaaggaat ttatttaatc
    16441 aataaagcat acgacttacg agataaatca agagaagaat tagcaaaagg aatctttgat
    16501 agaagtacag gagttatcaa ttttgtcacc tatgaatctg gagtgagtgc ttatgatgaa
    16561 acagggtttg gtgggcgcta tgaaccttat caagaaacga ttgaagatgt gatgaagttt
    16621 tcagataaca atgatggcta tgctcattta gagatggtgt tcgatgaaaa tggatttagt
    16681 ctagatgata aaaccagatt gcgattgttt gattttgagg gtcaacagat aggactaaac
    16741 aagttttgtg aaagcggaaa agagtttgct acgaaagatt ttattaaggg atacagatta
    16801 ttgctagaaa attatggaat aaacctagaa gaagagagcg tgaaataagt gtttgaaaaa
    16861 agaaaagacc agttccctaa tttagaagcg aaaagtcaaa aagtgattga tggtgaaatg
    16921 attgaattat tccaaaaaag ttatggaacc atagaaacta ccgcaagttg caaatactac
    16981 tttcaaggaa agagaaacac gtttaaaggc tttgccgatt ctgatgaaga attggcaaag
    17041 aaaatcattg attggcttgt cagcagtgaa cacgtagcaa gagaagatac cgttagaaca
    17101 acgattaatg gggaagtcct aacaggtact agatgtgttt gttgtaatca tgaatttgta
    17161 gaagatgaag gttgctaccg aatcaatgaa tatggtgatt ttatttgcca gagttgccac
    17221 gagatagaat ttccaaccga aagaagttgg tttgaataca tgctacaaga ttatgagatg
    17281 gaaacagaaa atggtgaagt agttttttgt accaaagagg aattgagtaa gagaagcgat
    17341 gatgaaattg aagattggtg tgtgtggtta gtagggacag atgattgcgt tgcatattac
    17401 acaacagcag aataggagtg tgaatcagca tggaaggaat ggattttata gatcatgaag
    17461 atttgattga ttttggttat acatggaaag gaatggtagg tattagcaga agtcttgcta
    17521 atgcctttta cgaaagaaat tatgcagtct atgtccttta tgatgacaat acagaaagcc
    17581 ttgttgatga agagtataaa ttggatttag agaatgtttt atatgggata gaaaaagaag
    17641 atttagcaaa atatattttt tcatggttgg gtcaataaac gaaagagggt agcaacatga
    17701 caaaattcaa aagtaaaggt atgcaatggt gttcttatta ttatgttggg aatgatgaag
    17761 agcttttgag aaaagccaaa caaaaaggtt atccagataa gatgttaaaa cagattggct
    17821 attacatgcc agaagagaga atagaaaaag aatcttgtaa ggttcttttt gatagtgaac
    17881 agctattagt tttgttaact caaaataaat tgatttgtca atttgaaaat aattaatatg
    17941 gggtgaagaa ccatgaatga gaataagatg gtattaagtg aatacgcaag attgagaacc
    18001 attaattggt tgaatggtat gcaaagtagt ggcgcttttg atggatttga tgaggagaat
    18061 atggactttg aaagtttatc gaatgaggat attagatatt attttatcga atggtatttt
    18121 acagaaccag atgaagaggt ggaagatgaa ccagaattaa ttttagaacg tcaaagaatt
    18181 ttattcagtg aacaagatag taagaggttg tatggtttat atgacttaca actgaaagga
    18241 ttaaaagcgc agtatgttgc tgatagttac gatgatatgg tagaacaggg ggcagatagg
    18301 gttgtcactt attcagaaga tgatgaagag gaatgggaaa actattcaga tgatgagata
    18361 tgctttaatg atgggtatat gcctattgaa atcaccgaag aagaatatga taaagtgtcg
    18421 gatagcgatg agattggtat gtgtacggtt gtaagtggat tggatataga tgtccaaaga
    18481 gtagaggttg cataccagaa gatgaaagaa cgtataaaaa gtaaagtaga atcttgattt
    18541 gattggattg tttgcaaagt agctaattaa gagtataata taatcataaa tgtagttaat
    18601 ggatataata atgagtatat tatactcaaa agaggtgaac gaaatgaagg taaaaaatcc
    18661 aaaagaaaaa agcagaattc aagtcggaat agacaaggat ttaaaagaaa acgcagaaat
    18721 gatattggaa gagctagggt taaatccaac aacagcaatc acaatccttt ataagcaagt
    18781 ggtcgctaga ggtgagtttc cagtagagat aaagctaagc gaagaggaaa agcaaggaat
    18841 cagattgcaa cagctaacta tggatatgcc agttgatgtt ctagatacag atgaaaagtt
    18901 agaagagtgg ttcaatgaag cctaatgaga tagtaacggt ctatgttgca tttacagata
    18961 aaaatggtgg taaaagaaga cctattttgg tggttagtga taaagaagat agagtagaat
    19021 tttttggaat cacaagtcaa tatgagaata aatcagacca aataaagaga gtgtattttc
    19081 ctattgcaga atgggaaaaa gtaggtctaa agaagcaatc atggattgac gtagggtctt
    19141 taaaggctat tcccaaaagc cgagagaaca tatcatttaa aaaggttggt atgctatcag
    19201 ctattgatac gaaaaagcta aataggttta tcgagagatt acaaagaaaa gtctaacaag
    19261 gcttttcttt ttttgtttat atgtatatta agatatacgt ttatataaac gtatttatgt
    19321 atattaaaat acacgtaaat atatacgtga ttataaaaat taagaaaata ttaagtattg
    19381 ttaaatagtg ataattagta atatacgaat tataagatac attttatatt taatgtatgt
    19441 aaataaataa aatgactgac ggatattgaa tagtaataaa taacaataat tcagacggat
    19501 acagtgagtg aagtagcttg ctacgaacga acaggcagaa tgaaacggta gtttcattag
    19561 agcaagtcgc ttgcgacgta gcgttaccac tgatgtaact ggcaagttac atagagagag
    19621 tttgcttgca aacgaacgtt acccacttga atagaaaaca aaaataacat attagtgaat
    19681 tttgaatagg agccacaact caaccaaccg cttgcggtgg gtgagtccca cggaggaaaa
    19741 gacgaacgaa cggtcgcgt
//
LOCUS       DAZKCC010000053        19735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000053 DAZKCC010000000
VERSION     DAZKCC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.054"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8VH_001932"
     CDS             <1..878
                     /locus_tag="ACG8VH_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9110482.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            complement(1119..2735)
                     /locus_tag="ACG8VH_001933"
     CDS             complement(1119..2735)
                     /locus_tag="ACG8VH_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9110483.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2853..3071
                     /locus_tag="ACG8VH_001934"
     CDS             2853..3071
                     /locus_tag="ACG8VH_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110484.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3266..3727
                     /locus_tag="ACG8VH_001935"
     CDS             3266..3727
                     /locus_tag="ACG8VH_001935"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9110485.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3920..4159)
                     /locus_tag="ACG8VH_001936"
     CDS             complement(3920..4159)
                     /locus_tag="ACG8VH_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110486.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4183..5706)
                     /locus_tag="ACG8VH_001937"
     CDS             complement(4183..5706)
                     /locus_tag="ACG8VH_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9110487.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5724..5846)
                     /locus_tag="ACG8VH_001938"
     CDS             complement(5724..5846)
                     /locus_tag="ACG8VH_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9110488.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5876..6859)
                     /locus_tag="ACG8VH_001939"
     CDS             complement(5876..6859)
                     /locus_tag="ACG8VH_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9110489.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG8VH_001940"
     CDS             complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG8VH_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9110490.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7053..7430)
                     /locus_tag="ACG8VH_001941"
     CDS             complement(7053..7430)
                     /locus_tag="ACG8VH_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110491.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG8VH_001942"
     CDS             complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG8VH_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9110492.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG8VH_001943"
     CDS             complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG8VH_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9110493.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8448..10214)
                     /locus_tag="ACG8VH_001944"
     CDS             complement(8448..10214)
                     /locus_tag="ACG8VH_001944"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110494.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10174..11928)
                     /locus_tag="ACG8VH_001945"
     CDS             complement(10174..11928)
                     /locus_tag="ACG8VH_001945"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110495.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12038..12607)
                     /locus_tag="ACG8VH_001946"
     CDS             complement(12038..12607)
                     /locus_tag="ACG8VH_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFP9110496.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12625..14040)
                     /locus_tag="ACG8VH_001947"
     CDS             complement(12625..14040)
                     /locus_tag="ACG8VH_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110497.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(14051..14719)
                     /locus_tag="ACG8VH_001948"
     CDS             complement(14051..14719)
                     /locus_tag="ACG8VH_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9110498.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14849..15433)
                     /locus_tag="ACG8VH_001949"
     CDS             complement(14849..15433)
                     /locus_tag="ACG8VH_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9110499.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15764..15997)
                     /locus_tag="ACG8VH_001950"
     CDS             complement(15764..15997)
                     /locus_tag="ACG8VH_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9110500.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(16012..16962)
                     /locus_tag="ACG8VH_001951"
     CDS             complement(16012..16962)
                     /locus_tag="ACG8VH_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFP9110501.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16959..17801)
                     /locus_tag="ACG8VH_001952"
     CDS             complement(16959..17801)
                     /locus_tag="ACG8VH_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFP9110502.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(17803..18522)
                     /locus_tag="ACG8VH_001953"
     CDS             complement(17803..18522)
                     /locus_tag="ACG8VH_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9110503.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            19070..19360
                     /locus_tag="ACG8VH_001954"
     CDS             19070..19360
                     /locus_tag="ACG8VH_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110504.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            19432..>19735
                     /locus_tag="ACG8VH_001955"
     CDS             19432..>19735
                     /locus_tag="ACG8VH_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9110505.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITI"
BASE COUNT         6429 a         3709 c         2796 g         6801 t
ORIGIN      
        1 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
      181 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
      301 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      361 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
      661 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
      721 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
      841 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
      961 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     1021 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     1081 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     1141 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     1201 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     1261 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     1321 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     1381 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     1441 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     1501 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     1561 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     1621 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     1681 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     1741 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     1801 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     1861 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     1921 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     1981 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     2041 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     2101 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     2161 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     2221 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     2281 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     2341 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     2401 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     2461 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     2521 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     2581 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     2641 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     2701 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     2761 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     2821 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     2881 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     2941 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     3001 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     3061 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     3121 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     3181 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     3241 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     3301 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     3361 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     3421 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     3481 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     3541 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     3601 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     3661 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     3721 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     3781 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     3841 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     3901 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     3961 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     4021 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     4081 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     4141 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     4201 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     4261 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     4321 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     4381 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     4441 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     4501 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     4561 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     4621 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     4681 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     4741 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     4801 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     4861 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     4921 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     4981 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     5041 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     5101 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     5161 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     5221 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     5281 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     5341 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     5401 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     5461 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     5521 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     5581 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     5641 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     5701 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     5761 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     5821 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     5881 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     5941 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     6001 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     6061 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     6121 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     6181 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     6241 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     6301 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     6361 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     6421 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     6481 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     6541 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     6601 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     6661 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     6721 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     6781 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     6841 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     6901 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     6961 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     7021 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     7081 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     7141 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     7201 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     7261 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     7321 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     7381 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     7441 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     7501 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     7561 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     7621 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     7681 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     7741 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     7801 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     7861 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     7921 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     7981 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
     8041 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
     8101 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
     8161 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
     8221 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
     8281 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
     8341 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
     8401 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
     8461 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
     8521 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
     8581 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
     8641 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
     8701 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
     8761 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
     8821 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
     8881 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
     8941 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
     9001 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
     9061 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
     9121 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
     9181 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
     9241 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
     9301 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
     9361 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     9421 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     9481 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     9541 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     9601 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     9661 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     9721 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     9781 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     9841 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     9901 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     9961 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    10021 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    10081 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    10141 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    10201 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    10261 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    10321 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    10381 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    10441 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    10501 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    10561 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    10621 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    10681 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    10741 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    10801 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    10861 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    10921 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    10981 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    11041 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    11101 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    11161 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    11221 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    11281 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    11341 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    11401 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    11461 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    11521 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    11581 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    11641 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    11701 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    11761 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    11821 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    11881 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    11941 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    12001 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    12061 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    12121 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    12181 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    12241 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    12301 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    12361 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    12421 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    12481 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    12541 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    12601 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    12661 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    12721 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    12781 cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc
    12841 acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa
    12901 taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag
    12961 ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc
    13021 taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa
    13081 cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt
    13141 catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac
    13201 atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc
    13261 aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa
    13321 aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagttgtagt
    13381 ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca aatagtaaaa
    13441 acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc
    13501 aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata taattaattt
    13561 agagtttagc gataaactac atccaataga aattttctgc cgttcaccac ttgatagcga
    13621 aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt cattgattct
    13681 tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta actcactcgt
    13741 tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa tattttttga
    13801 tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt ccccaaccaa
    13861 ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg tggatttccc
    13921 actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa aattaaggtc
    13981 aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa attcaaacaa
    14041 ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac aggatgatat
    14101 agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga gattctatcc
    14161 cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt ataagaacgg
    14221 gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga atgtttctca
    14281 tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag aatctgatca
    14341 ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa gaggataata
    14401 attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc ggcaacattc
    14461 gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat
    14521 caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa atacatgtct
    14581 ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc acttgaaata
    14641 tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta atcaaggtta
    14701 acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg ttatcttaag
    14761 ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag cataactgag
    14821 aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac ccaggaacag
    14881 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
    14941 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
    15001 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
    15061 ttttcgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
    15121 ggaataaaat caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
    15181 attcattgaa gttctctctc caacttttct ctatatctat attttctaag tttaagaggt
    15241 atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc tgaataattt
    15301 cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta gttacgcctg
    15361 cggtcaggca cagttgcctt atcttagttt gctgaaatcc ttctttttca aaatggctta
    15421 gtccactttc tattaacttt tggtgagtta taatataatc tctggtttct atgggtactt
    15481 cctcctaata agatgaatta actttattat cttacacagg acttgaagtt tcaatcgtta
    15541 acgtttatta ctgaactcat tccttagagc taaactaatg agaaagagca gaataactga
    15601 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
    15661 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
    15721 actccaatag ccattttctt atcctcccta aaaataaagt cttttactta aaaccttttt
    15781 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
    15841 cctcatatct aagtttagcc agcatctcac ttacagatgc tggactaaca cttagaactt
    15901 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
    15961 aataaattag tatagtagtc gttttaattt gtaccatacc taccctccaa attatttcga
    16021 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
    16081 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
    16141 ttttgatact gttttcattg gtctatcatc cacactgctt tctacgaata aggagggaac
    16201 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
    16261 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
    16321 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
    16381 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
    16441 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
    16501 accgttttct aaatttaacc aagcatgagg atcttccttc cctttctcac tctgaccctc
    16561 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
    16621 cgcattttta actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
    16681 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
    16741 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
    16801 ttcggtaata tctgctaaaa tcgaattagt aactactact tttaattttt cgttcgaagc
    16861 cactttatct ttgtctttac tatttattga acatgccccg aatataaaaa gagcaatcaa
    16921 aatccccaga cttcccagta ctgctttcca ttttctcatc ataacgatct ctccttcttt
    16981 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
    17041 aaaacaatac ttgatcctgc tgctatgtta aaactataac caataaacaa tccaagcaat
    17101 gatgaacttg ctccgactgt tgcagaaata aatatcatag ttttcaagct atttgcatat
    17161 aaatatgctg tagctgctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
    17221 tgcatagctg ttactgctac taaagttaat agaaccatca atagataatg ataaaaactg
    17281 acattcatcc ctattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
    17341 aaaaatagaa ctactataac caacactaaa accgaaacgc caattgtaat ccacttatca
    17401 atatcttgta cagctaaaat atttccaaat aaaatatgaa ataaatccgt tgagctattt
    17461 gcaactccta taaggataac tcctagtgct aaaaaagaac tgaaagtaat accaattgct
    17521 gtatctcctt ttattacact attgcttttt ataaaagtaa taatgataga agctaataga
    17581 ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa tataagaaat tgctactcct
    17641 ggcaaaacag catgagaaat agcatcaccc ataagagaca ttcctcttaa aataataaaa
    17701 caacctactg ttccagaaac aatccctata actacagatg taattagagc attctgtaaa
    17761 aagtgaaagt catacagacc attaataaaa ttttgaagca tattattccc cctctccaat
    17821 aaatatagta tctccatagg ctttttttaa attttcttca ttaaatacag attctacact
    17881 tccgtgagca attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt
    17941 gcttaaatca tgatgcacaa ttagcactgt ttttccctga tgtttcaatt tttttaacgt
    18001 atccataatt attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat
    18061 gaaatccgca ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga
    18121 taattctcct atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga
    18181 aactctttgc cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc
    18241 catagataca cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc
    18301 tacatatgca atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc
    18361 ctgatgaggg ataatattta acattgcttt aagcaatgtg gattttccag caccatttgg
    18421 accaataata cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa
    18481 aaaatcgtta taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat
    18541 gataatgatt atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt
    18601 tatagtttag atttttttct gttgctgaag attagaatga caaaactatt tgaaactacg
    18661 cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg agaacatgct
    18721 cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag cgggttgaat
    18781 aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg ttccatatac
    18841 aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt
    18901 ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca
    18961 atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt
    19021 agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc
    19081 agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
    19141 tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg attttataga
    19201 atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac
    19261 tctagataag tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg
    19321 ctttgacgta gaagatatta aagactttat gaatacttga aattttcttc tcgccgcata
    19381 tactaaaatt agtgaaatat actgccttac tttttttaag gagtgaggat tttgcctaca
    19441 aagctttcaa atgggaaata taaaactaat ctgagatatc caaaaaggtt taaagaaata
    19501 acaggaattg catcagaaaa gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa
    19561 gccgaaaatg atatgaagaa gaaaatagaa aaagttttga gagaggagaa tgcaaactca
    19621 ttagaattaa aaggaaaaat aacgtttaaa aaattttacg aatctaaatg gcttcctaga
    19681 tatgaattag gtcaaacaat tagatcaaac aggcctccta gtgatataac catta
//
LOCUS       DAZKCC010000054        19694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000054 DAZKCC010000000
VERSION     DAZKCC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.055"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG8VH_001956"
     CDS             complement(<1..210)
                     /locus_tag="ACG8VH_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9110506.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG8VH_001957"
     CDS             complement(216..446)
                     /locus_tag="ACG8VH_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110507.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG8VH_001958"
     CDS             1063..1725
                     /locus_tag="ACG8VH_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110508.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8VH_001959"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8VH_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP9110509.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG8VH_001960"
     CDS             5145..5459
                     /locus_tag="ACG8VH_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9110510.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG8VH_001961"
     CDS             5472..5846
                     /locus_tag="ACG8VH_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9110511.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG8VH_001962"
     CDS             5856..6200
                     /locus_tag="ACG8VH_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP9110512.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG8VH_001963"
     CDS             6271..7623
                     /locus_tag="ACG8VH_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9110513.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG8VH_001964"
     CDS             7683..8825
                     /locus_tag="ACG8VH_001964"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9110514.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG8VH_001965"
     CDS             8850..9104
                     /locus_tag="ACG8VH_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110515.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8VH_001966"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8VH_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9110516.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG8VH_001967"
     CDS             10541..10678
                     /locus_tag="ACG8VH_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9110517.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG8VH_001968"
     CDS             10683..10904
                     /locus_tag="ACG8VH_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110518.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG8VH_001969"
     CDS             10917..11417
                     /locus_tag="ACG8VH_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9110519.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG8VH_001970"
     CDS             11514..13361
                     /locus_tag="ACG8VH_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP9110520.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACG8VH_001971"
     CDS             13364..14260
                     /locus_tag="ACG8VH_001971"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9110521.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACG8VH_001972"
     CDS             14310..14699
                     /locus_tag="ACG8VH_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9110522.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACG8VH_001973"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACG8VH_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG8VH_001974"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG8VH_001974"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9110523.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACG8VH_001975"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACG8VH_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAZKCC010000055        19480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000055 DAZKCC010000000
VERSION     DAZKCC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.056"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8VH_001976"
     CDS             133..3660
                     /locus_tag="ACG8VH_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9110524.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8VH_001977"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8VH_001977"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP9110525.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8VH_001978"
     CDS             7448..7789
                     /locus_tag="ACG8VH_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9110526.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8VH_001979"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8VH_001979"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9110527.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8VH_001980"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8VH_001980"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9110528.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8VH_001981"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8VH_001981"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9110529.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8VH_001982"
     CDS             13000..13737
                     /locus_tag="ACG8VH_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9110530.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8VH_001983"
     CDS             13751..14566
                     /locus_tag="ACG8VH_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9110531.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8VH_001984"
     CDS             14579..15226
                     /locus_tag="ACG8VH_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9110532.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8VH_001985"
     CDS             15242..15898
                     /locus_tag="ACG8VH_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9110533.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8VH_001986"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8VH_001986"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9110534.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8VH_001987"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8VH_001987"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9110535.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8VH_001988"
     CDS             18240..18758
                     /locus_tag="ACG8VH_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9110536.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8VH_001989"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8VH_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP9110537.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4182 g         5402 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DAZKCC010000056        19350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000056 DAZKCC010000000
VERSION     DAZKCC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.057"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..1299)
                     /locus_tag="ACG8VH_001990"
     CDS             complement(274..1299)
                     /locus_tag="ACG8VH_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9110538.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1574..3259
                     /locus_tag="ACG8VH_001991"
     CDS             1574..3259
                     /locus_tag="ACG8VH_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9110539.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3273..4556
                     /locus_tag="ACG8VH_001992"
     CDS             3273..4556
                     /locus_tag="ACG8VH_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9110540.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4626..5495
                     /locus_tag="ACG8VH_001993"
     CDS             4626..5495
                     /locus_tag="ACG8VH_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9110541.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5492..6328
                     /locus_tag="ACG8VH_001994"
     CDS             5492..6328
                     /locus_tag="ACG8VH_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9110542.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6354..7421
                     /locus_tag="ACG8VH_001995"
     CDS             6354..7421
                     /locus_tag="ACG8VH_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9110543.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7418..8245
                     /locus_tag="ACG8VH_001996"
     CDS             7418..8245
                     /locus_tag="ACG8VH_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9110544.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8290..9315
                     /locus_tag="ACG8VH_001997"
     CDS             8290..9315
                     /locus_tag="ACG8VH_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9110545.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9312..11495
                     /locus_tag="ACG8VH_001998"
     CDS             9312..11495
                     /locus_tag="ACG8VH_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP9110546.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11495..12625
                     /locus_tag="ACG8VH_001999"
     CDS             11495..12625
                     /locus_tag="ACG8VH_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9110547.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12622..13299
                     /gene="pgmB"
                     /locus_tag="ACG8VH_002000"
     CDS             12622..13299
                     /gene="pgmB"
                     /locus_tag="ACG8VH_002000"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9110548.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13383..13910
                     /gene="msrA"
                     /locus_tag="ACG8VH_002001"
     CDS             13383..13910
                     /gene="msrA"
                     /locus_tag="ACG8VH_002001"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9110549.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14111..14797)
                     /locus_tag="ACG8VH_002002"
     CDS             complement(14111..14797)
                     /locus_tag="ACG8VH_002002"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9110550.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14877..16415
                     /locus_tag="ACG8VH_002003"
     CDS             14877..16415
                     /locus_tag="ACG8VH_002003"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9110551.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16535..17002
                     /locus_tag="ACG8VH_002004"
     CDS             16535..17002
                     /locus_tag="ACG8VH_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9110552.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17536..17964
                     /gene="rplM"
                     /locus_tag="ACG8VH_002005"
     CDS             17536..17964
                     /gene="rplM"
                     /locus_tag="ACG8VH_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9110553.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17980..18372
                     /gene="rpsI"
                     /locus_tag="ACG8VH_002006"
     CDS             17980..18372
                     /gene="rpsI"
                     /locus_tag="ACG8VH_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9110554.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18460..>19350)
                     /locus_tag="ACG8VH_002007"
     CDS             complement(18460..>19350)
                     /locus_tag="ACG8VH_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9110555.1"
                     /translation="LPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMF
                     DIAEILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFN
                     NHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGR
                     KDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYL
                     NYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNT
                     PDIVDKAVFEAFQRGLNKCD"
BASE COUNT         6252 a         3507 c         4110 g         5481 t
ORIGIN      
        1 atccccaaat aacatggatt gaaataaggg taaatgtgtt taaaaaaacg aactataaaa
       61 acttatacac aacgtggtta aaaatagttt tttcttccct gtggaaaaaa tagtttaaca
      121 caaacattat agttatccac agctatctaa aacagatgga aaataagaaa aattcgctaa
      181 aataccggga gggggtaacg agggatgagt gattgaaaaa agaacagagt aactgactta
      241 tcctcagtgt ttctgttctt ttataattct gttctacatt tcttgttgtt ggatcgaatc
      301 tgtgagagta atctcggttg aaagaatgat tcttttttct tcagggtatt ggtggtggat
      361 tagttggaat aaaagttctg tggctttgat tcctaactga tttgtcggaa tgtctatccg
      421 agtcaactgt ggcgaaaata gggaggacaa taagggatca ctgaatcctg cgatcgaaag
      481 atcatcaggt atctgctttc cagattgttc tgccactttg taaatactca atgattttaa
      541 tgcatcggta gaaaaaatcg cagttggtct ttctcgtagc ttcattaaat cattcattgc
      601 tgccatgcta tcttctaagc gaaagccgcc gtctataatc cattcattac ggatatctag
      661 gtggtgatca aataagcatt gacggtaacc attgagacga tcattggata cataaacttc
      721 tgtcggagca tggagacagc cgatatgacg gtgtccattc ttgatcagtg tttccaccaa
      781 atcgtaacta tcctggaaat tatccgtgtc cactgagtag atgttcgtgt agtcgtttcc
      841 gacttttccg attaccacaa taggaatatt gtattgatcc aattcaaaaa gaagtgtttc
      901 atccatcgta gagcttaaaa taataattcc tcggatcatt ttttcttgga tctttttcgc
      961 gcatttttct agctcttctt ctggtgtttt tgccgtttgt agaatcaagt cgaagttatt
     1021 ttgttcagaa acggcagaaa tggattgaat aacatctgaa aaaaaaggat tgccataggt
     1081 cgtatgagaa gtacgatgag ataatgctag aatcgcgtca aaaccatttc ctgacagtcc
     1141 tcttgcaatt ttgctcggtt cataattcaa ttctttgatt gcttgaagta cacttgcttt
     1201 tgctttatca gagatatttg gctggttatt taatactctg gagacagttg attttgagac
     1261 attagctttt ttcgcaatgt cataaatagt aggtttcaat tttatatgca cctccttttt
     1321 gggagcgttt tagaataatt ccatagtaca atagacattc ttttttgtct atgattttga
     1381 aaatttagga atagcttgca tgatcatgag agaccatatt tttttaagaa gctattaatt
     1441 attgatacat aagctttctt atttgtattt ttgtgtatat caaaattatt taaaaaaatg
     1501 ctattgacag taagcgtttt ccataatatg atcaaatcgt tgggaacgct cccaaaaaag
     1561 aaaggggaat ccgatgtata caaaactttt acaagcgatt tatcgcgact cagatttgtc
     1621 tgagaaaacc gcctctatga ttgaacaaaa agtcagtgtt tatcagcaga agaaaggcca
     1681 ggcaggcaaa ctgcattggg acgaagaaga tattttcttg attacttatg gggatcaatt
     1741 ttttgaagag cagcaaccaa ctttgcgtac atttaagaaa ttttatcaaa agtatttggc
     1801 agatacattt agagttgtac atttcttgcc atttttccca tatacatctg atgatggttt
     1861 ctccgttgta aattatcagg caatcaacga ccgtttgggc acatgggaag atatagaaga
     1921 aatgagaagc gaaacacggt taatgttcga ctttgtctgc aatcatatgt ctgccgaaag
     1981 tacttggttt caggaatatt taaacttgaa tgatacgtac aaagattttt ttattgattt
     2041 gccactggat acagatacaa gcagcgtgac tagaccaagg accagcccgt tacttacaac
     2101 gttcattgac aaaaacggga aagaaaaaca cgtatggacg acttttagcg aagatcaagt
     2161 agacttgaat tttaaaaatc cagaagtttt attgaaaatg atcgatattc ttttgtttta
     2221 cttagaaaaa ggcgcttcat ttattcgctt ggacgctgtg ggatttttat ggaaagaact
     2281 aggaacttct tgtatacacc tgccagaaac gcataaaatc atccaattat tccgtgcaat
     2341 cgctgatgaa gcagctccag gaacggtatt gatcactgag acaaatgtgc cacacaaaga
     2401 taatatcagt tattttggga atggcgagaa tgaagcacag atggtctatc agttttcgct
     2461 accacctttg acccttcaag cgattcgttc gggtaataca agtaagttga aagaatggtt
     2521 aaaaaccatc gatttcgcat cgaaaaaaac aacttttttt aattttttag cctcccatga
     2581 cggtatcgga ttgaatccgg tacgaggtat tctgccggaa aaaacgatcc ttgaattagt
     2641 ggaacagctg aagaaagagg gtacccgagt aaactacaag aaaaacccag atggaacgac
     2701 cagtccatat gagataaacg ctacgtatct agatgcgtta agttctccaa cagatacgac
     2761 ggaatggaga agcaaacgct ttttagctgc tcattcattt ttggtaacgt taccaggcgt
     2821 gccggcaatt tatattcaaa gtgtgctggg aagtcgaaat gattacaaag gcgtagaaaa
     2881 aacaggagag aatcgaaaaa tcaatcggca aaaatttttg ttagcagata ttgagaaaga
     2941 gttgcagaca gatccattaa gaacagcagt atatggtgaa ttgacccaga tgctatctat
     3001 tcgaaaaaaa gaaaaagcct tccatccgaa tagtttgatt tacttactgg actcagcaga
     3061 ttcttttatc agttttattc gcgaagcgaa ggaagagaaa gtcttagtta tccataattt
     3121 ttccaatcaa tcagcaggtt atcttttacc aaaaggagat tacctagacc tgttttctgg
     3181 acaaattatc cattgcagtc aagaacgaaa gttgatattc cagccgtatg aatatatgtg
     3241 gctgaaaata ataaaataag aggtggaaaa aaatgaaaaa aattattgtt actacctgtc
     3301 tgctagctac tggatttctt cttactgctt gtggtgaaag tcaaagctct aacgaatcac
     3361 aacagacaac gattcaattc atgcactctt ctgtcgaaca ggaacgatta agtgtgatca
     3421 atgaactggt tgctgatttt gaaaaagaaa atccagacat caaaatcaag caagtgccgg
     3481 tagaagaatc agcgttcaat acaaaaatcg ttacgttagc tcgttccggt aaattaccag
     3541 aagtaatgga agtcagtcag gattttgcta aagtgatgga taaagatgaa ctgattgatc
     3601 agacggcagt ccaaaacgtt atcgaggaag ctggagaaga taattattat gacggagcca
     3661 aaaaccttgt tcgttcagaa gatggaaaaa gctatattgc tgcaccaatt agcggctggg
     3721 ttcaaggtat ttggtatgac aaagaaaaat tatcagaagc tggtttttca gaaccagaaa
     3781 attgggatga tattttaaaa attgctcaac atttcacaga taaagagaat aaacaatatg
     3841 gaattgctat gcctactgct gaaggaacaa tgagtgagca agcgttctct caatttgctt
     3901 tgtcaaatgg agcaaatgtt ttggatgatg aaggcgaggt aacgatcgat acggaaaaga
     3961 tgagagaagc attgtcgttt tatcaaaact tatcccaata cacgatgcca ggctctaatg
     4021 atgtgacaga aatcaaagat gctttcatga atggaacagc accaatggca atctattcaa
     4081 cttacatttt gccttctgtt tatgaagcgg ggaactcaga aaatatcggt tttgctatcc
     4141 caacacaaaa agatcaagct gtctatggca cagtatctgg attgacgatt tcttctggtc
     4201 tagacgagaa acaaaaagag gctgctgaag cattcacagc tttcttatct caaccgaaaa
     4261 acatggaaaa gtgggtcttg atgtctcctg gtggtgctca accagtgaat aaacaagtag
     4321 tcgaactgga tggttacaaa gaaaatgaag tcatcaagtc ttttggcgag ctgccatctg
     4381 agattgctag tgcttttgat gaagtacaag tctttggact agtcggggaa aagaatttca
     4441 cgaaaatggg agatatcaca agctcaggtg tgatcggaaa agcagtgaac caagtaactg
     4501 tcggaaacga agatgtcgat caagctttag ctgacgcaca aaaaaatacg aaataacaag
     4561 aagttctgcc aatggtgttc gaaaaaagta gatagctatt ggcagattat cagggggagg
     4621 tttcaatgaa tcgtccaagg aaacattctg acttaaaatt agccatggga cttttagcac
     4681 caagcatgct tttattagct gttttagtgc tgattccaat gctttccaat attcagatca
     4741 gttttttcga tatgccgctg aatcctaact cagacgccgt ttttatcgga ttaagcaatt
     4801 atcttgaaat tttaagtgat agtgaatttt atcgttcttt aggtttaaca gttgcttaca
     4861 cattcttagt tgtagcaggc agtacagctt taggattagc cgtagctatt ttgcttaacc
     4921 gcaagttcag atttaggaaa acggtgcgtt cactgatttt attaagttat gttacgccat
     4981 ctatttcttt gatttttgct tggaaatata tgttcaataa tagttatgga atcgtcaatg
     5041 atctgttagt agataggttg cagcttttca acgaagcacc gctgtggttc gaccagccag
     5101 ttgctagctt ctttatggtc gttttgtttg ctatctggag atactatccg tatgcgttta
     5161 tttcattttt ggctattttg caaacagtcg acaaaacgct ttatgaagca gctgaaatgg
     5221 acggtgcaaa tgcttggcag aagttcaaga tcgtgacact gccggcaatc aaaccagtac
     5281 tagcaactgt gattgtttta cgatctatct ggatgttcta tatgtttaca gatgtctatc
     5341 tactgacgaa taaagtcaat atcttaggcg tttatttgta tcagacagcc tttgcgttca
     5401 atgatcttgg aaaagcagca gctatatcga ttctattgtt catcattatt gcagcggtcg
     5461 tcttgctctc acgaaagaag gtggacttga aatgaaaaat aaaaagagca ggaaaatctt
     5521 attttacgcg acgattttgt tgctattagc tgtcaatttg tttccatttt tgatcatgct
     5581 gatgacatca ttcaaggatt cacaagaagc aattgcaacg acacaaacta tttttccgaa
     5641 agaatggact tttgaacatt atcaggatat tttcaatgct gagatttttc cattcatcat
     5701 gtatttcaaa aatagtttag tgatctcctt gatagcctcc gtaactgcag tagtgattgg
     5761 gatcagcggg gcatacgctt tgtcaaaact acgcttcaaa gctcgctcga aaatcaatgc
     5821 tagcttttac accgtttata tgttttccgg tattttgatc gttgttccat tatttaaaat
     5881 catttccggt ctaggtttgt atgataccaa tacagcactg attatcacga tggtcgttca
     5941 aacacttcct actgcgatat tcatgctaaa aagttatttt gacacgattc cgactgatat
     6001 tgaagaagca gcaatgatcg atggtcttag ccggatccag atcattattc ggatcattat
     6061 accattggct atttctggaa tcatttctgt cttcgtttat tgctttatgg tttcgtggaa
     6121 tgattattta tttgcttcga ttttcctgtc gacctccgaa aaatttactt taccaatcgg
     6181 tttgaacaca ttattcagta caccagacta catttgggga cgaatgatgg ccgcttcatt
     6241 ggtgaccgct ttaccagtca tcattatgta tgcgttatct gaacgattta ttaaaggtga
     6301 tttagtcgaa ggcggcgtaa aaggataaaa agaaaagaaa gaggaaagaa aagatgaaaa
     6361 aattagttgc attagaacca agaatagccg gattcaaaga ttacgaagac cgtgagatcg
     6421 aaaagaacga agtacgtatc cgagtaattt tcgctactcc aaaaccagga acagaaattg
     6481 tcgactttag aggattaagt ccatttatcg atgaagaatt ttcagaagaa tggcgcctat
     6541 tcttgccaag agaagaagga agccaacgcg gtgtggtatt tggcgaattt caattaggaa
     6601 atatgattgt cggtacgatc gaagaaacgg gcagtttggt gacagagtat caaaaaggag
     6661 agttagtctg cacttatgga cccattcgtg aaacgctgat tacaaacggt gttgataatt
     6721 ataaactacg taaacttgat cggttggatc aatggaaaaa tgcagtatgt tacgatcctg
     6781 ctcaattcgc tttaggtggt atccgggaag cacatgttcg agcaggtgat gctgtagcaa
     6841 tcttcggttt aggcgctatc gggcaaattg cgattcaatt agctaaaaaa gcaggtgcta
     6901 ctaccatcgt tgggatcgat cccatagcgc agcgtagaga attagcagaa aaatacggtg
     6961 ccgattacac gctagatcct atgaaagcag atgtcggttt tcgtctaaag gaattgacag
     7021 ataaacgtgg agtagattca gttattgaaa cgagtggcag tgctgccgca ttacaagctt
     7081 ctttaagagg gatcggctat ggcgggatca tttcttatgt tgcctttgct aaaccttttc
     7141 cagctgggtt taatcttggt cgcgaagcac atttcaataa tgcaaaaatc gtctttgctc
     7201 gagcatctag tgaaccaaac ccagattacc caagatggaa ccgtaaacga atcgaggaca
     7261 catgttggga attattgatg aacggttact tggattgtga agaaatcatt gatccagttg
     7321 tgccatttgc agaaagcgca gaagcttaca tgcgttatgt agaccagcac ccagaagaaa
     7381 gtatcaaaat gggcgttcaa gtccgaggag gagaacaatg aaattcggta cgcaagatca
     7441 agcatttttc ccaagagaca ttttagaaaa atttagattt atcaaagact gtggatttga
     7501 agaatacgaa atcgacggta agttgttaat agaaaatatc gacacagtca aacaggcaat
     7561 caaagaaaca gggatcccag tagtgacagc ttgtaacggc tacgatgggt ggatcggtga
     7621 ctttatcgaa gaacggcgtc ttaatggtgt acgtcaaatt gctgagattt tgcgtgcact
     7681 aagcgaggtt ggaggcaaag gaatcgttat cccagcagct tggggaatgt tcacttatcg
     7741 attaccgccg atggtttcac cccgaagcaa agaaggagac tataaagcag tcagcgaatc
     7801 acttcgttat ttagataaag tagccgaaga aacaggtacg gttatttatt tggagccttt
     7861 gaaccgatac caagaccata tgatcaattt gttagcggat gcgcgtcatt acattgactc
     7921 taatcaattg aaacatgtgc agatcatcgc tgatttttat catatgaata ttgaagaaga
     7981 tgatatttca aaagcattga ctacgcaaaa agagtatgtg ggtcatatcc atttagcaga
     8041 taatcatcgc taccaaccag gatctggcgc aattgatttt actcgccatt ttcaaacgct
     8101 acttcaaaat gagtatgaag gttatatggt atatgaatgt cgtttgcgag gagaaaatga
     8161 agtgaaaatt tatcaggaat cattgcaata cttgaaaaac tgtctggaaa cagcgcgtca
     8221 tttaaatgat ttagagaaag aatagagaaa gaataacaat aagtaaataa gcaaaggagg
     8281 gagaaataaa tgaagaaagt agcaatcata ggcgctggac aagtagcaga aaaagtacat
     8341 gctgcttatt atcaaaaaag atcagatgtc ttagaattgg ccgctgtggt tgatccagtc
     8401 agccagcgag cagaagactt ttgccgacgt acaggatttt ctcatgcgta tacatcagtt
     8461 acacaaatgc tgaatgagat ttctcccgat atcgtgagtg tctgtacacc caatcgcttt
     8521 cacttcgaac atgtattggc tgccctcgaa gctaatgctt cagtgatgtg cgagaaacca
     8581 ccagctatga cagctgatca agcaagaaag atggcagaga cagccgataa aaacgggcag
     8641 attttggctt atggattcca gcatcgtttt tctgacgaag caacaatgat caagaatcat
     8701 cttgatgttc tcggggatat ttattacgta gaagcgaatg cgcttcgtcg gtctggtgtt
     8761 ccaggctggg gaacgtttat cgacaaagaa cttcaaggag gcgggccttt gatcgattat
     8821 ggcattcaca tgcttgatac agcgatgtat attactggat ttcctaaagt aaaaagcgtt
     8881 cgggcgtaca gcttccaaaa aattggtacg aaaaaaaatt cagggaaatt cgggaaatgg
     8941 gatccaagca aattcacagt agaagatgca atttttggca cactggaatt agaaaacggc
     9001 aagatcatcc aactgaatac ttcgtttgct ttgaatatca gagaagacaa actgaatgtg
     9061 gctttttgtg gcgatcaggc aggtgctaat ctgtatccag gcgaactatt cgaagatgta
     9121 gatggaaagc tggtttcctt acaaaagacc acgccagtta atgacccaca tgaactaagt
     9181 atggctcatt ttgtgaatcg ggcgctcggt gaccagactc agatgattgc agatggctgg
     9241 caaggatatg tcatccagaa tctggtggaa aatttatatc gttcggcaga aacaggagaa
     9301 gttattacct tatgaaagta gaagaaaaac agtttgattt agagtatatc aataagtatg
     9361 cgagtcagat gaccattgga aatggatttt ttggtatcag aggaagccag gaggaagact
     9421 atcggaatca agtaagggga atgtttgcag ctggtgtata taaccgacct ttaggagcag
     9481 aatcagccga gttagtgaat ctcccagatg tcatcagatg ggaaataaaa atcgacggtg
     9541 aacatttttc tcttgagacg atgaaagttc ttgcatacga gcgggcacta gatttagaaa
     9601 tgggagagct taaacgctat gcgctcattg aaactgctca aggaaaaaaa gtggaagtca
     9661 ttacttgtcg tgttgctgat caaaataatc tccatcaggc agccctgaaa gtggtcatca
     9721 agccgttgac cgaagactgc caagtagatg tgaaaacggg tatcgatgga cagcaaacca
     9781 attttggtac acagcagttg gttgaaaaag aactgcgagt atttggtgac gatttgatca
     9841 gtgcatccta ccagacaaca gaaagtaaaa ttgatattgg gattgcagct aaattcaata
     9901 aagcaggtgt attccaagca aaaaatcgcc ggatcaccgc ttccttttca gaaaagatca
     9961 gaaaaaatac gtcctttacg ctggagaaga tcatcgtgct ctctacctct cttcagcaaa
    10021 cagacccgtt taagaaaagt attgcccaag cccagaaaag cgttagttat acagaaattt
    10081 tagagcagtc gatgtcttat tggacagatt tttggcatca gcatcggata acgatccaag
    10141 gcgattcttc tttcgatcag ttagcgatgg acttcgctgc ttaccattta cagattatga
    10201 ctccccgcaa tgatccaagt gttagtgtag gggcgaaggg actaactggc gaaggttata
    10261 aagggcatgt tttctgggat acggaaattt ttatcctgcc tttcttttta tatcagtttc
    10321 ctgaagtagc aaagaatttg ctgctgtacc gttatgatcg attagaaggc gcacggcaaa
    10381 aagcaagaga ttatcactat caaggtgcac ttttcccatg ggaaagcgca cacactggtc
    10441 aggaagagac acccaaattt gctgcattga atatcaaaac cggaacaaga caagaagttg
    10501 cttcagctgc cgcagaacat catatcagtg cagaccttgc ttatgccgtt caagagtatg
    10561 tccaagcaac agaggatcaa tcgtttttag aaacaaaggg aaaaacgctc attcatgaaa
    10621 cagcagcttt ttggctgagc agagctgtaa aagtcgatga tcatttagag atacacagag
    10681 tcattggtcc agatgaatac acagaatata tcgacaataa tgcatatacg aactatctgg
    10741 cgcattataa tgtggcctta gctgttaaat atcaagctgg cgatccgctt tttttgaaag
    10801 aatgtcaaaa ttttttagat aagttatatt tgccagtgcc taacaaagaa cagctgattc
    10861 cgcaagatga cacgtatctc agtaagccag ttattgattt gaccacttac aaagaacgtc
    10921 ctggcagcca gtctatttta cttgattatg cccgtagcga agtaaatgaa ctccaagtca
    10981 gcaagcaagc agatcttgtc atgctgttat acttattacc tgagtatttt tctcaaccaa
    11041 tcgtttctaa gaatttgcat tactatgaaa acaaaacaat ccacgactca tcgttgagta
    11101 aagcgattca cgccatagag gcttgccgta tccaagaaat ggaatgggcg tatcatttat
    11161 tccaagaagc gtgcctgatt gatttaggac cagatccgca ttcatcagat gacggaattc
    11221 atgctgcttc attaggagca ctatggctag cagttatatt tggtttttca ggtattacag
    11281 taacgaatgg aactctttct atttctccaa gacttccttc agcatgggaa aaaatttctt
    11341 ttccttttat ttggaaaaat cacaggttgc tatttgtttt gacaaaaaaa caaatagtaa
    11401 taaaaaaaga gacgaaagaa acattaccta tttttgtcaa tggagaccaa taccagctgg
    11461 aaaaagaact gatcttggaa cggaggggaa aataatgcgt attctgattg cgccagattc
    11521 tttcaaagaa agtctgacag ccttagaggc agcacaagcc attcaatcag gttttcaaga
    11581 agtttttgtt caggctgaat atgatctgct acctataggc gatggaggag aaggaacggt
    11641 ttcatcatta gcagacagtt taaggttgaa gcggcaaaca gcagtcgtta ctaatgcatt
    11701 tggcaaaaac aaagaaatcc agtacgcttc agacggtcaa actgcgattt ttgaaatggc
    11761 agaaatcgtc ggattgaaag atgtcccact aaaccaacaa cagccactta ggttatccac
    11821 gcgaggagta ggagaactga tacgacaatt gttgcaacaa ggtgttcgac acatcatcat
    11881 cggcgttggc ggaagtagta ccaacgatgg cggcatcggc atggcagaag gtctaggtta
    11941 tgctttttac gatcaagata atcaaaaagt agaagcactg ggagaaaatc taggtaaaat
    12001 tgctacagtt gataaaacga acgtgcctat tgagctgatt aaagccaaaa tcactgtcat
    12061 tactgatgtt actaatcttt tatgcggaaa agaaggcgct tcttttgttt tcggaccaca
    12121 aaaaggcttg aaagaaaatg aattagcagt ggctgaccaa gccatgcatg atttttatat
    12181 gagatttgcc cctcaagtaa tcgaggcatc gggcagtagt gctggtggtg gaatgggtgc
    12241 aggactgtta gcatttgctt cagggaaaat agtctctggc atcgactacg tattggattt
    12301 actggagttc gataaacggg tacagcaagc agacctcgta atcgtaggcg aaggacgtct
    12361 agatggacaa agtctaaaag gaaaagcgcc agttgggatt gctagaagag taccagaggg
    12421 cgttcctgtg atagctattt gcggaagtgt tggtaaagga agcgaaaaag cagcagatgt
    12481 tggcatttcg gccgtttttc cgatccttcc tgcagctgtg cctttacaag aagctttaca
    12541 gcaagccact gataatcttc atcgcacagc taaaaacatc gcatctttac ttcaagtcgt
    12601 tcattttcaa ggaggtaatc gatgaaaatc aaagcagcac tattcgattt agatggcgtt
    12661 cttgtagaca cagctcgtta tcattacgaa gcgtggctgg ttttagctaa ccagctgtcc
    12721 attccattca ctgagaaaga aaacgagcaa ctgaaaggaa tatccagaac cgagtcacta
    12781 gagcgattgc tgtcatttgg aaaaatggaa cagaaattct cagaaaaaga gaaatcagcc
    12841 tttgctgagc aaaaaaacaa tctatatctt caagcgattc aaaaaatgga tgaaacatcc
    12901 gttttgcctg gtgcaatagc tgttcttgag tacttgaaaa aaacaaacat caagatcgga
    12961 ttgggatcag caagcaaaaa tgcacgcctc attttagaaa aaacgaattt gacaagttat
    13021 tttgatgtcc ttattgacgg cacgcaagta tcaaaagcaa aacctgatcc agaagttttt
    13081 ctcaaaggag cacagcaatt gaatgtaccg ccaaatgcat gcttggtcat tgaagattca
    13141 gaagctggtt gtcaagcagc tttggctggt aacatgcatg tattgggaat aggtgaaaac
    13201 ataaatcttc cttctgctga atatgtgatt cctgatttaa ccgtttttga tcaagtacgt
    13261 tcgttttggc atttatcaga agccgtatca cgtatataat agtttccgtt ttttctgtca
    13321 caagcaaaac gttatcctcc ttttggaaat caaggtatga tgagaaaaaa gggggatttt
    13381 atatgacaaa aaaagcagtt ttcgcaggcg gatgcttttg gtgtatggtc aaaccatttg
    13441 aagagcagcc agggatcatt tctgtgacat ccggttatac tggcgggcat gtaccaaatc
    13501 caacttatga acaagtaaca acaggcacaa cagggcatac agaagcagta gagatcgaat
    13561 atgatccaga agttatttct tatgatcagt tggtggaaat ctattggcag caaaccgatc
    13621 cgactgacgc attaggacag ttcgctgatc gcggtgattc ttatcgtcca gtgatctact
    13681 ataatgatga agaagaacga aatattgcag agcgttcaaa acaagcattg caagacagcg
    13741 ggcgattcga taagccaatc gtgacaaaaa tcgaaccaag aagcgaattt tatcctgcag
    13801 aagattacca tcaggattat tacaagaaaa acaagctcca ttacaatctt tatcgagaag
    13861 gttctggacg tgcaggtttc cttcgtcgac attggggcgt caagaaataa gaagccgaaa
    13921 aagctactta tggaacaccg tatatcagag gttgtaaaac cagcattttg agctaatact
    13981 gagggtcagc tgattgaaca ccatttagtg gagaggttgt gaacctgtcg cctagctcca
    14041 agtcacaagg atttggtaag agaacactat atatcagagg ttgtgacaag tcgtttgtca
    14101 caaccttttt tcaataccta agatgaagga caatcagcaa cgcactgctc aaaaaaggaa
    14161 tgaaaggcag cttttccaac ttttttttat gacagataaa gtaaacagaa aaaacggata
    14221 ttccaaacag actagcaaga aacaagatct gaagcaactc ttctagagac aagccaccac
    14281 tccaagaaag catcaacaga gtatcgccag ctccgagata ggattgacca tagcgaaggc
    14341 acacaccgcc tacgataaac gttaacagca cactttgcca ttggaagacg ccggcctgca
    14401 accaaaaaat ccataaaatt gcccaagaac agaatagcgt gtgagaatgg accatgaagt
    14461 aaaataaatc catcaacgaa aatagcaaag ccgaaagcat ccatatgaac gtcgaaaaat
    14521 ttcttgcgga agaaaaagta aagaaccagg caaaaagacc accacataag gcttctgcca
    14581 acaaatagta gaagggcaac cgacacctgc aatagcggca gcgaaatcgt tggacgatga
    14641 tcgagagaat cggaatcaat tcataccaag aaagcgaacg acagcatttg acacaatgag
    14701 atctaggata aataatagag tgacctaacg gaatccgctg ggcggttaaa cataaaaatg
    14761 atccgataca gcaaccaatg ataaaataaa gtaacacgcc atctccccct tttcaaaacc
    14821 aagatacgca agcttaaact aaatttttgt acatacataa aatttgaggt gaaaaaatga
    14881 acgtttcaat cggattagat atcggaacca cacaaaccaa agcagttgct ttcacagatg
    14941 aagcccaaga agcagcatcc gcttattttc gctacccttt gatccaagaa acacaaggga
    15001 tggcagaaca agatcaggaa ttgatctttc aaggtgtctg caccgttata aaagaagtaa
    15061 ttaaacagtt gccttcagac acaatcattc aatttgtctc cttctcctct gcgatgcatg
    15121 gcctgatatt gttggatgaa gagaaaacac cgttatctcg aatgatcact tgggcagata
    15181 accgagcagc ggacgaagca gaaaaactga aatataccgt gcttggcaaa gaattttatc
    15241 ggcagactgg gacgccgatt catccaatga caccattgaa caaaatcaag tggctgcaaa
    15301 acagccaacc tgaattattc aaaaaaactg cttattttgt cggtatcaaa gactatatct
    15361 tttatagatt gtttggcgaa ctgatcagcg actacggaac agcttcagga actggatatt
    15421 ttgatatcca tgaattccaa tgggcagaag aagtgcttga ctacttgaac attcgaatag
    15481 agcaattgcc gcaagtcttt ccttccacct atcaagtaac aggtctacca tcaaaaacag
    15541 cagcagaact aggtttgcca aatgatatat catttgtttt gggaggggca gacgggccgc
    15601 tttctaatct agggttaggt gcttttggtg atacgatcgc cgctttgacc gtaggaacga
    15661 gcggagcact tcgttttatt actactcagc ctcagttcca tccagaaatg gagacgtttt
    15721 gttatgttct ggatcagaaa cattgggtag tgggaggagc gactagcaat ggtgctggca
    15781 tctttgattg ggcagggcat acatttttac aggaagtcgt tcaatctgct aaagaaaaag
    15841 gagaaaatcc atatgatgca ctgctttcgg ctatcgcttt tgtgccagcg ggtgccaatg
    15901 gtctgatttt tcatccctat ttacttggtg agcgagcacc gctttgggac gcagatgcgt
    15961 ttggcagttt tatcggttta cagcggcaac atacagaaaa agaaatgatg aaggcgattc
    16021 tagaagggat ctgtctcaac ctttatcgga ttttaaaagg agtttgccag gaaaatcagc
    16081 tcacagagat ccgtgctaca ggcgggtttg ctcaatctcc gatttttcgt cagatgatgg
    16141 ctgatgtact gggttacccg cttgttttcc ctactgtcac agaagcatcc gcattaggag
    16201 ctgtgatttt aggctggaga agcatcggaa agatccaaag tatcagtgaa gcacaggaat
    16261 tgatagaatt gaaagagcaa gttggagtac aagaaactga tcataaaaaa tatcagaaaa
    16321 tctatccatt atttgtttcg acacaaaagc atttagcaca gacttatcag caatttgctc
    16381 aactgcgaga agaattagct gattctgaaa aatgattggc tttacggata aaaaagcgaa
    16441 ctgaaaatcg ttctcaggta aatgattttg ttgtactttt cattgaaagt ctactattat
    16501 tttaagtgta agaaaatgtt aggaggcaag aaagatgaaa ttccaacaaa ccaaagaagt
    16561 attaaatcaa ttagttgcag atctaagcca aatgtctgtt gttgttcacc aaacacattg
    16621 gtacatgaga ggaccaggtt tccttacatt gcacccaatg atggatgagt tcatggatga
    16681 tttgaacgct cagttagatg aagtatccga acgcttgatc acattagacg gttcacctta
    16741 ttctacattg cgtgaatttg cagataacac aaaaatccct gatgaaattg gccgctggga
    16801 tcgtactatt ccagaacgtt tggaaacact tgttgcaggc tatcgttatc tagcagatct
    16861 ttaccaaaaa ggaatcgaag tttctggaga agaaggcgac gatccaacac aagatatctt
    16921 tatcgaattc aaaacagcca cagaaaaacg tatctggatg atccaagctc atttgggcaa
    16981 agcgccagag atcgacgcgt aaaaactgtt gttgaacttg accgaaaaag aggttgtgaa
    17041 atcagcgttt tgacctgagt attagagaaa caaacgtcaa aatagttcct catattttgc
    17101 cgttttttga gctaataccg agggtcagct gattgaacac cgtttaccaa gaggttgtaa
    17161 gcctgccgtt cagctccgag tcacaagaaa caattttaaa aaacagcttc ttatgtttta
    17221 tggatattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt caaaataagg
    17281 ggttgtgaca agacttttgt cacagcctct ttttgttttt cattttgaca ggaaacgacc
    17341 cttttatata aaagaaagaa aagaagtaaa gtttcatata aatttttcaa tcaagcagaa
    17401 aaacgttgac attgactaga ttagcgggta atatgttaaa tggtattgtt tgccccacaa
    17461 agagccccgg aaactcgaag tgtatgtcaa acatcagaac ttaaattgga ggaaaataac
    17521 gtgcgtacaa catatatggc caaaccaggc gaagtagaac gtaaatggta tgtagtagac
    17581 gcaactgatg ttccattggg acgtctttca acagttgttg catctgtact acgtggtaaa
    17641 aataaaccaa ctttcacacc tcatttggat actggcgatt tcgtcatcgt catcaatgca
    17701 gacaaagtga aattaacagg taaaaaagcg actgacaaaa tttattaccg tcacagcaac
    17761 tacccaggag gattgaaatc aatcactgct ggcgaattgc gtgctaaaaa ttctcgtcgt
    17821 ttgatcgaaa cttctgtaaa aggtatgctt cctaaaaaca ctttaggccg caaacaattt
    17881 acaaaattga acgtatacgg tggagcagaa catccacatg cagcacaaca accagaagtt
    17941 ttagacatca caaacttaat ctaataggag ggaatttcat tggcacaagt tcaatatatc
    18001 ggcacaggcc gtcgtaaaaa cgcagttgct cgcgtacgtc tagtaccagg aactggcaaa
    18061 attactgtaa ataaaaaaga tgttgaagaa tacattccac atgctgactt gcgtgaagta
    18121 atcaatcaac ctttcgctgt tactgaaaca aaaggcgcat atgacgtatt tgttaacgta
    18181 aacggcggag gatacgctgg acaatcagga gctatccgtc atggtatcgc tcgtgcattg
    18241 ctagaagttg atcctgactt ccgtttagct cttaaacgtg ctggcttact tactcgtgac
    18301 gcacgtatgg tcgaacgtaa aaaaccaggt cttaagaaag cacgtaaagc ttcacaattc
    18361 tcaaaacgtt aatatccttt atatatcaac gtttctggca cttttcaatt atttggaaag
    18421 tgcttctttt gtttttaaga acacatttaa gaacacatat caatcacact tattcaatcc
    18481 tctctgaaaa gcttcaaaaa cagccttatc aactatatct ggtgtgttca catatatctt
    18541 tgttgtagaa atatctgagt gtgttaaagc atttgaaatt tgattcatat ctgctccacc
    18601 ttgtctagct atagttgcat aagtatgacg gagtttgtgt ggagataaat gaactaagtc
    18661 aggatgcctt cttttaattg aattgattcg atagtttagg taatcggcat gaacaggaca
    18721 atttacttct ccagatggtt tagtataagt aaagagatac tgatctaacg tttgcttaat
    18781 ttttaatttc aacaattgat cagcttgtac cgatttccat tcacttagca atgtcattac
    18841 aacttctggt acttgaatga cggtatcttt tcgacctttt gtaaactttt ttctttgata
    18901 tttatctaaa gtatgtttaa gacggaccgt ttgtttttca aaatcaatat atttccattg
    18961 caaagcgtaa gtctcacttt ttctatcacc aagatacagg gtaatcataa aaagtgtgta
    19021 atcatgaaaa gtaagtaaat gattattaaa atcatcattt actgcttcta tccaattagt
    19081 aagttctttt gatgatagtg cttgcttacc ttcttgtatt cttttttctt ctaaagcatt
    19141 ttttttaggt gcagtgatac tttgaataat tttagtagtt cgattaaatt cgatataatt
    19201 tagtatttct gcaatatcga acatggaacg aacatatcct tttatgattt ttatatttgc
    19261 atattctttc gattttttgg ttaattcatc tgatattatc tcagtattga tatttagata
    19321 attcattgcg tattcaccaa acataggtaa
//
LOCUS       DAZKCC010000057        19068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000057 DAZKCC010000000
VERSION     DAZKCC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.058"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..805
                     /locus_tag="ACG8VH_002008"
     CDS             <1..805
                     /locus_tag="ACG8VH_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP9110556.1"
                     /translation="SAISKDEVEKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQ
                     GLIEEARQNPVAGKENVALYAFANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAAL
                     MGHALFSRPDLYQQLKNDFSTLQDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEG
                     SWVILDLYGTDHDERTVEAPDSFMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCA
                     GEWITLHSLRVFSDQLVNKFEFSVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(894..1841)
                     /locus_tag="ACG8VH_002009"
     CDS             complement(894..1841)
                     /locus_tag="ACG8VH_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9110557.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2235..3527
                     /gene="hisS"
                     /locus_tag="ACG8VH_002010"
     CDS             2235..3527
                     /gene="hisS"
                     /locus_tag="ACG8VH_002010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9110558.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            3542..5311
                     /gene="aspS"
                     /locus_tag="ACG8VH_002011"
     CDS             3542..5311
                     /gene="aspS"
                     /locus_tag="ACG8VH_002011"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9110559.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5420..6295
                     /locus_tag="ACG8VH_002012"
     CDS             5420..6295
                     /locus_tag="ACG8VH_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9110560.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            6561..8444
                     /locus_tag="ACG8VH_002013"
     CDS             6561..8444
                     /locus_tag="ACG8VH_002013"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9110561.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(8519..8803)
                     /locus_tag="ACG8VH_002014"
     CDS             complement(8519..8803)
                     /locus_tag="ACG8VH_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9110562.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(8793..9527)
                     /locus_tag="ACG8VH_002015"
     CDS             complement(8793..9527)
                     /locus_tag="ACG8VH_002015"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP9110563.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            9656..10279
                     /locus_tag="ACG8VH_002016"
     CDS             9656..10279
                     /locus_tag="ACG8VH_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9110564.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            10471..11592
                     /locus_tag="ACG8VH_002017"
     CDS             10471..11592
                     /locus_tag="ACG8VH_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9110565.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            11599..14727
                     /locus_tag="ACG8VH_002018"
     CDS             11599..14727
                     /locus_tag="ACG8VH_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9110566.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            14746..17940
                     /locus_tag="ACG8VH_002019"
     CDS             14746..17940
                     /locus_tag="ACG8VH_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP9110567.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18086..18793)
                     /locus_tag="ACG8VH_002020"
     CDS             complement(18086..18793)
                     /locus_tag="ACG8VH_002020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9110568.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6348 a         3381 c         4038 g         5301 t
ORIGIN      
        1 atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag
       61 cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga
      121 aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa
      181 tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga
      241 agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc
      301 ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga
      361 cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc
      421 aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg
      481 cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc
      541 agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga
      601 atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga
      661 atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt
      721 ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag
      781 ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa
      841 acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat
      901 ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct
      961 tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt
     1021 ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca
     1081 atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct
     1141 ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt
     1201 cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca
     1261 agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact
     1321 acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca
     1381 ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt
     1441 tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga
     1501 cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc
     1561 cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct
     1621 ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg
     1681 gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca
     1741 tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt
     1801 tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg
     1861 tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt
     1921 cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa
     1981 tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat
     2041 ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt
     2101 acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga
     2161 atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa
     2221 agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg
     2281 aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt
     2341 ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg
     2401 atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg
     2461 tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt
     2521 tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg
     2581 aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat
     2641 cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt
     2701 tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag
     2761 cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg agtgaagact
     2821 ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc
     2881 aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac
     2941 attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta
     3001 atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac
     3061 caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag
     3121 aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct
     3181 tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg
     3241 tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact
     3301 ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag
     3361 cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa
     3421 cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat
     3481 atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata
     3541 aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt
     3601 tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct
     3661 acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg
     3721 ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag
     3781 acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag
     3841 tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc
     3901 aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac
     3961 taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa
     4021 tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga
     4081 ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca
     4141 attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg
     4201 tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga
     4261 aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt
     4321 aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga
     4381 agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga
     4441 tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg
     4501 cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat
     4561 ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt
     4621 agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat
     4681 cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga
     4741 aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat
     4801 tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga
     4861 agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat
     4921 cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa
     4981 cggttatgaa ttaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt
     5041 actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac
     5101 agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc
     5161 aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa
     5221 agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt
     5281 gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga
     5341 atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca
     5401 tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac
     5461 gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt
     5521 ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac
     5581 tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa
     5641 tatacctttg tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt
     5701 tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac
     5761 cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc
     5821 gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa
     5881 aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc
     5941 cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg
     6001 aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa
     6061 acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat
     6121 caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac
     6181 acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt
     6241 aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga
     6301 gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt
     6361 tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt
     6421 gaaaaaaagc atgtttattt ttttaaattt atgggatgat agaaaaaaat agtagagaaa
     6481 ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg aaaaaagttt
     6541 atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca
     6601 ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat caccttctgc
     6661 caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta tctggttatt
     6721 tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat atttggcaat
     6781 tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt gtgacagctt
     6841 cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga gttgtggtca
     6901 gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct tatttcgatc
     6961 gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa ggtcaaaact
     7021 atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga
     7081 tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg atcttgtata
     7141 caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg ttttctatat
     7201 ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa aacatcccga
     7261 aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca ctgattcttt
     7321 gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg ctcctagtga
     7381 gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt ttgaatcgat
     7441 ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt tatctgtatc
     7501 agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat catgttttgt
     7561 tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct tatcgatatg
     7621 tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg aaaggatggt
     7681 tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca ttacttgtag
     7741 caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac
     7801 aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga aacaattcag
     7861 aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg tccaaggcac
     7921 aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt atgttggatg
     7981 ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat gtcggacgac
     8041 aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg ttgaaagaca
     8101 ctgttctgat tggactagga acgaatggct cattctcgga agctcagttt gatagtgtga
     8161 tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg acgaagcgct
     8221 ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac aatatgacgc
     8281 tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat gatcaagtcc
     8341 atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc ttgctaggag
     8401 gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta caaaaaactt
     8461 caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt tttaacattc
     8521 atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct ttttgttttc
     8581 gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt
     8641 ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta gcaccagtac
     8701 cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca taaaatgtct
     8761 gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat ctcacgaatc
     8821 tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac tttgaatccg
     8881 tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt tccaggtttt
     8941 gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac ctgaatgatt
     9001 tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt tagtagctta
     9061 cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc gattgccagg
     9121 tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg attacagacg
     9181 actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc gatggtgtgg
     9241 acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat gtctgctaga
     9301 cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca
     9361 actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac acgggaaaag
     9421 tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg gataatcttg
     9481 atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa
     9541 aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca aggtggcttg
     9601 tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag agactatgtt
     9661 ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg gaaatgcccg
     9721 ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg ctccccatcg
     9781 tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat tcagctttat
     9841 ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc gatccaatgc
     9901 ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg taaagatttt
     9961 gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg ctacagaact
    10021 taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc cagctgtata
    10081 tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag tgcgtttcgg
    10141 cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg cggagatcga
    10201 acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata
    10261 tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg aatctactgt
    10321 cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt
    10381 ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa gtgttataat
    10441 aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc cgattggcat
    10501 atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt actatcttct
    10561 atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg agacttatat
    10621 gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat ggtcgattgg
    10681 aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag cagtacgaga
    10741 ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac acagctagca
    10801 gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc ttactttgaa
    10861 ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa gcaagcggta
    10921 gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca agtattgatt
    10981 agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc
    11041 gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc actaggacat
    11101 ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc cccattgaaa
    11161 ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc
    11221 ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt
    11281 ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa ttacatctat
    11341 atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg gcaaatatat
    11401 cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga
    11461 aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag tgaaatgacg
    11521 gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca agaaatcatg
    11581 gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt
    11641 atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt gatcagcggg
    11701 aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact ttttggtgaa
    11761 acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac accagacgaa
    11821 gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat cgaacgaaaa
    11881 ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc agccaaagta
    11941 acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa
    12001 gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc tcaaatcgtt
    12061 ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga taaagagact
    12121 gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg attaaaagaa
    12181 aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca tcttctgcaa
    12241 aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc cgttttagcc
    12301 caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa agaagctcta
    12361 acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt tgaaagcttg
    12421 aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt agctgctcaa
    12481 aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac gcaacgatta
    12541 gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact gtcacaaaaa
    12601 agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga gtggcaaaaa
    12661 aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa
    12721 aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa
    12781 caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta tcaaaatctc
    12841 gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca ggaaaaactg
    12901 caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca gcaagaaata
    12961 gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga actcaaaaaa
    13021 gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga acaagcagaa
    13081 cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca
    13141 ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt aaaacacgaa
    13201 cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc tgagtctgct
    13261 gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa tcttcaacaa
    13321 caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc tgaacaagaa
    13381 gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa
    13441 acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat ggccaatgca
    13501 agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca attagctgtc
    13561 tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg agaaatcaaa
    13621 agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga gatacgacga
    13681 cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa agcaggagtg
    13741 caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca tgaacaaata
    13801 gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc
    13861 cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca agcacaatat
    13921 aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac agctgaccgg
    13981 ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga agagatcagc
    14041 caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat atacggttta
    14101 caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca
    14161 agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac attcaacgga
    14221 gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct tcaagaagtg
    14281 ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca atttgagcta
    14341 gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat ctatgatgat
    14401 caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt tattgcagca
    14461 ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg tgtagcaatc
    14521 gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt ggaaatggca
    14581 atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta
    14641 agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg tagcggacaa
    14701 agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa atggagtatt
    14761 ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt gttatcggtt
    14821 gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga attgtatatg
    14881 gttgatttgg atggtacagc aaaagagata gattactgcc aatgtcccta ttgggaggaa
    14941 cataaatatt gtaagcatac agttgctgtg gaactttatt tacggtcgaa aaatctatct
    15001 cgtatcatga aaaaagacca gccaatgcta gaaccggtaa aagcaacaag tgagggagaa
    15061 atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct
    15121 ttgattattg aataccaagt agaaacgatt gagactaatc agtatcaccc agagctttct
    15181 attttggcac tttacttgcg tatcggttca ttggcatcac cgaaaaaaac atatatcgta
    15241 aaaaacatct atgatttttt acaagcttac acagaaaaag aaacttacac tgttaataaa
    15301 cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa aatactcacg
    15361 catcttgcag gaagtgctca gacgcatcaa ttattaggta cgaacggctt acaagtcaaa
    15421 ggaaaactag ataaaagata tctgcttctc ccagtagaac aagcccgctt cctgttagag
    15481 cagatgaatc agactacacg tttgcatctg cagataaatg aacaaaacga tcaaggaata
    15541 caattttccg atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg agaagattat
    15601 ttgctgcaag cattgaatga ttttgatttt ttctttatgc attatcagtg gggagttatc
    15661 caagggacat tttattctct gacgaaatat caacaaacgg tttatcaaac ttggaaacaa
    15721 ttgattcgcc gattggagaa accagaagta gtgttcaaga aaaaagaatt accgtctttg
    15781 tttaaagaga ttatccctct gctgtcagag atcggcgacg tagctattgc ccctgcagta
    15841 gaagaggaag tagcagatat ccccgtcgaa tttattttct ttttccgaaa agcaaaagga
    15901 aaaatccaag ctcgagtcga tttcgtctat ggtgaagtca tctattcgac tgacccgaag
    15961 catgaggtct cgacagaaca ggcttttcag gtgttacgtg acacagtaga agagaaacga
    16021 gtacttgatc tattcagaat gtatcgctac cagaaaaatg aaactggtta tgaacgtcca
    16081 ttgccaagcg gggaagaact atatgccttt ttccgcacag aactatctgt cttccgtcag
    16141 tttggtgaag tcagacttgg aagaaaattg cgagagttgt atttagatgc gcataaacat
    16201 caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg atattcgatt cgatgtgaca
    16261 gggatagagg agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt
    16321 tatacattag aaaatggtca ggttctttct tttgattcag aagaatttca acaaacaagc
    16381 caagtgttgc aacaattacg ggaatcgata cgtacagaag aaggaacgat ccatgtacct
    16441 aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa
    16501 tcgttccaaa cgatggtaca agatctcatt catccagaaa gataccaggc acagcttccc
    16561 aaaggattga atgcaacgat gagagattat caaaagcaag gttttcgatg gttgaagatg
    16621 ttaggtcatt atcagtttgg cggtatttta gccgatgaaa tgggacttgg gaaaacacta
    16681 cagacaattg cctttttact ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc
    16741 gtagcgcctg cgagcttaat ttacaactgg caggcagagg tgagaaaatt cgccccttct
    16801 ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg aagaattact ggctaaagac
    16861 acagatatcc gagtgacttc ttatgccagc ttaagacagg atctagctaa ttatcagtcg
    16921 caaaaaattg attatttgat tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa
    16981 actgcacaag cattgagaga attagctgta cctcagcggt ttgcactgag tgggacacca
    17041 atcgagaata atttagaaga attgtggtca ttgtttgcaa cgattatgcc aggatttttc
    17101 ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg
    17161 tttattttac ggcgagataa acaaactgtg ttgaaagact tgccggaaaa aacagagatg
    17221 aatctatatt ctgcacttac agaagaacaa aaaactgttt atttagctta cttgcgtcaa
    17281 atgcgtgaag aaatcacatc aatggattct gaagcattca agaaaaaccg gatcgggata
    17341 cttgctggtc tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat
    17401 taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc tactggtagc tgccaaagag
    17461 aacaaacgaa gagttttgct gttttctcaa tttacaggga tgctgactat cttgcaagaa
    17521 gagttagccg agctagggat ctcgacattt tatttaagag gcagtacgaa accacaagat
    17581 cggttaagta tggtagacgc atttaatgct ggcgaaaaag atgtttttct gatttcctta
    17641 aaggctgggg gaactggatt gaatctcacg ggagcagata cggtcatctt gtatgacctt
    17701 tggtggaatc ctgccattga agaacaagct gctggacgtg cacatcggat cggacaaaaa
    17761 aatgtggtag aagtgtggcg tatgatctct gaaggaacga ttgaggaacg catggattca
    17821 ctccagcaag aaaaacgcga actgttccaa aaagtgatcc aaggtaatga agaacagttg
    17881 acgaaactga ctgaagaaga catccgcatg attttaagtg taggagaaat cgaggagtaa
    17941 gagactcgaa aaagcgttca gtaatgggta agaaagcctt tctaaagaag cgattgttta
    18001 tttgatttaa aaaatagcaa ggaggctggg attccatttt atcccagctt ccttgctatc
    18061 atttttttat ttctattttt aaaggttata caaagattgt cttcattgtc tctaacagct
    18121 gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg ttcaattttg cgttctcgat
    18181 ggttgaacca aagcgcttgc cagttagcac tttttgcacc tagaacatca ttgtcgtaat
    18241 tatcgccaac gtataaggtc tcttctggaa gcatatgaaa ttctttctca gccagctgaa
    18301 aaatctcttt ttctggtttt tggaaacctg ttgcttgaga aatgagcata ttgcgggaag
    18361 gaatccaatt gttcaattga agttgcttca gtttttttgt ctgatggtca gtcggcccat
    18421 ttgtgataat tcctaatggg acattctttt ccttcaagaa atcgagggtt tttttgacct
    18481 cctcatgcaa gcagatatta tcaagttctt cctcgtaaat tttttgaaat aagaggccat
    18541 cttcttccgt aatatgaggg tagtctaaat cttttagtga ttggctgatc cgatgtgctc
    18601 gcatatattc tagtgtccaa tctcctgcca ttacttttgg gaagttttca tcactatgat
    18661 ggcggaaccg gatatatagt ggatgcatat ctgcatctga aacaagcgga acgactcttt
    18721 tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt atcatcaaca tcaaacacaa
    18781 ctgatttaat caaaatttta cacgcctttc ggttttcatt gattttttca caaatccttg
    18841 actattgtaa cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt tcttactata
    18901 tttctatttc cttgacagaa taaggttttt atatttcagt ttttttcact aaaaatcgtg
    18961 aaacacgtca ataagtttga tgaacaaatt aatcattatg gtagaagtcg attgatttat
    19021 tttaatgcaa tctctgtgga attttcgaaa gatttatttt tttaatac
//
LOCUS       DAZKCC010000058        18745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000058 DAZKCC010000000
VERSION     DAZKCC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.059"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..1042)
                     /locus_tag="ACG8VH_002021"
     CDS             complement(131..1042)
                     /locus_tag="ACG8VH_002021"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9110569.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1419..2057)
                     /locus_tag="ACG8VH_002022"
     CDS             complement(1419..2057)
                     /locus_tag="ACG8VH_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110570.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2061..4043)
                     /locus_tag="ACG8VH_002023"
                     /pseudo
     CDS             complement(2061..4043)
                     /locus_tag="ACG8VH_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4223..4888
                     /locus_tag="ACG8VH_002024"
     CDS             4223..4888
                     /locus_tag="ACG8VH_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110571.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5285..7318)
                     /gene="brxL"
                     /locus_tag="ACG8VH_002025"
     CDS             complement(5285..7318)
                     /gene="brxL"
                     /locus_tag="ACG8VH_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFP9110572.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7436..9958)
                     /gene="pglZ"
                     /locus_tag="ACG8VH_002026"
     CDS             complement(7436..9958)
                     /gene="pglZ"
                     /locus_tag="ACG8VH_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFP9110573.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10074..13706)
                     /gene="pglX"
                     /locus_tag="ACG8VH_002027"
     CDS             complement(10074..13706)
                     /gene="pglX"
                     /locus_tag="ACG8VH_002027"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFP9110574.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13770..17441)
                     /gene="brxC"
                     /locus_tag="ACG8VH_002028"
     CDS             complement(13770..17441)
                     /gene="brxC"
                     /locus_tag="ACG8VH_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFP9110575.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17466..18041)
                     /locus_tag="ACG8VH_002029"
     CDS             complement(17466..18041)
                     /locus_tag="ACG8VH_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFP9110576.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(18041..18640)
                     /locus_tag="ACG8VH_002030"
     CDS             complement(18041..18640)
                     /locus_tag="ACG8VH_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFP9110577.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5794 a         3568 c         2620 g         6763 t
ORIGIN      
        1 aaaaaaaatt tttaaaataa aaagaactaa atcaagtaat aattaaacta ctgttgattt
       61 agttctaata aaaagattta gatgaacagt tttattgatt taaatagttc atatttttta
      121 tttataccgc ttatttacta cttgctgtac tagaatctga agtgaatgta ctcaaaacat
      181 tttcaaagtc tttgtctttg atttttacat tagcgtcttt caatacttcg ccaattactt
      241 tattctggaa ggtactgtca cttaattttg tgtctgttgc aatttctttc aattcttttt
      301 tgtatttatc catgtcgttg cctttgtttt ggtttttcac cattttaacc acataatatt
      361 ccgtagtata agttgaagca ttggtagaag tgatcacatc agaaacttga ccatctttca
      421 acttaaaggc tgcttctttg acttctgccg gaaccgttgt agtagtggaa tcaaatttca
      481 cttttccgcc atcttctttt gtcgtagtat cagttgactt gtctttggct aatttagaga
      541 aatcgtctcc cttatcagca gctttcttca catcttttgc atcatcttca cttgctactt
      601 ggataatttg agcttccact tctggatgga aagattccca agccgttttt aaatcttcat
      661 cggtcaattt gatatgtttc tttagacctt cttgaaaggc tagactttgt ttgatactgt
      721 ctttgaatgt ttgttctgtg taacctgcag atttcaattg tgaatcaaat gaatcaccca
      781 attgtttctt tgtttgatca tattgggaat cgatttcttt ggttgatacg tcatctccat
      841 atttatcttc aaatacttga taaacgatca actgttgcac agcggaatct gctccttgca
      901 tttttttcaa gctatccatt acttcttgtt cgctgattgt accgccttta taactaacgg
      961 ctgttttttc accggcattc gtacagcctg acaataagcc agtgcctagt acgatggcaa
     1021 gtgctaaaaa tctttttttc atttatcaat tctcctactt ttcgtttttt gcgaattgct
     1081 aaaaacaaag gtgttcaaga tgaaaatcat caagaacacc tttactaatc tgctcgatac
     1141 gtcctgaata gatataaggc tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa
     1201 gtagcacatc acaccttaat atactacttc ttttgttacc gtttttcaag tctatcttgc
     1261 gttccgtatt taacgtaact aattcttaga acaaatgtta tcgattttgt ttttcttctt
     1321 ccgctgttgg gatattcaaa tcagacactt ttatgttcga ttttccagac atttttttgt
     1381 tgtttttatt agaaaaagtt tgttttttca tttgttttct aaagcatgat cttcttgttt
     1441 cctattttgg tcagatatgg atcatgcgtg accacgataa ctaacgtacc ttgggaagcc
     1501 tgttgcgata agatttcaac gacttgctcc ttatttcgtt tatctaatga tccagttggt
     1561 tcatcggcta aaatgatttt gctattcttc aataagactc tcgcacaggc gattctttgc
     1621 ttctcaccac cagataatgt gtagataggc gtacttagct tcttgtttat atttactttt
     1681 ttcaatgcct catactttgc tgttcgctta tctgaaacat ttaaattcga aaacttcaat
     1741 ccaatatcta aattaaaatc gattgtttca tattcaatca acccaaaatc ttgaaaaaga
     1801 aaagagaggg catttctttt tatcaagcgc ttctctcttc cagttaaatg ttttgtttct
     1861 tgattaaata aataaaattg ccctgaactg aattgatcat tcaatcctaa tatattcaat
     1921 aaagtacttt tccctgctcc gctctctcca actacagtca caaaatcccc agcatccgca
     1981 gtgaaacgat ccaaatgaag aatcgttttg tttcgaatga tttttttcgc accttttatc
     2041 actatcaacg gctccaattg ttaacctcca ttcatcagca ataactgact tttcttttct
     2101 gtagacaaaa taaaaacaac tgaaagaata aaatcaatga agctaaagaa aaaaatatta
     2161 aaaagatatt taggctctct ttctataaga aaaaggaaga ttacacttgt cgttccccat
     2221 ttgatgaggt tcatcatgaa gaaatcttta tacgtttcaa agagcgaata accacttgaa
     2281 cgaaagatag cgattttccg acaattgtag acaaaatata tgcataacat gacatagctc
     2341 aacaagaaga aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg aaatgatctg
     2401 cttaacactt tgacagctat ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta
     2461 tcacgtaact gactttcttc aatcagctca aataaccttt ctttcgacaa agagcctatc
     2521 ttgatcttta acggtggata gatttctccg ctgagaatat ttctttccca atacgtactg
     2581 tttttcaatg tcagtatttc aacaaccgga taatgttcga tccaggggat agaaggaata
     2641 aatgtataaa tgggttgatt gttttttata aatagaaatt tcggttcttt ttcactgatt
     2701 tcttgataat attctgttat ttgcgccagg tcatttttta aatctgtatg tctatatcgt
     2761 tcagggatca atataacagg gtgcgtttct ttttcggtta tttggatcaa ccttccagag
     2821 acatcaatca aaggaaattt cttaagataa ttcagattta tttttatccc tctgccaaac
     2881 tgttcatttt cttcattcaa gtaatctgat acattgacta atatacttcc gtttttattc
     2941 aaagtttcat ataattcctg atcggctgct gatgcaaaac gattggaata aataaaatcc
     3001 acgtggtttt tcccgatggt tactggataa aatacatgat agtcctcacc agcgatttct
     3061 tttggtgttt catgggaaac agctaactgt gaagaagaaa aaacgatagt tgctaattcg
     3121 acattggcaa cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag aatgtattgg
     3181 atatagttga acgcttctct taacgaagcg attttttcaa ctaataaaag agaaatcaaa
     3241 gagataccct atattccgct gaacaagaaa acgtacatct ggaacaaata aagaaattgc
     3301 tccttataat acataaaaat aaaaccacca atgattcctg caattatgaa tgtaataatg
     3361 attttctttt tccccaataa acaccacaat acttgatgta atgaaaagcc attattcaac
     3421 aataaactga tttctttatt catcaataag aaaaaggatg ataacaaaag gaataaaaga
     3481 atcaatccaa tagttaaata cactcgataa ttatatacgt ctgtacttga aataaccgaa
     3541 tacgaatagc tattttcaaa gtcttcaaaa atattagtgg cataatcagt gtgaaatgct
     3601 tgattgaatc gttccacaaa tagtaaaatg aaccgttcat aggcttcttt ttctctcgtc
     3661 atcaagaaaa attcaccttc aaaatctttt attttgatcg attcttcaat ggacgttacg
     3721 cgaaaggagg tattagaata gggtaccttc tcgtgagaag atttgacatc cccttgataa
     3781 tacaatgtca tttcaagctt tggctgtatc ttgaagttcc aagatgtatc tacttcattg
     3841 gattcattta ctgttttttt catataagaa agctcagttt ctgatgagac attttcaaat
     3901 accatgatta gctgctcata ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga
     3961 gtataagtat ctgtttgatg gaaaaattca tttgtccgat agagtacctg tttatttata
     4021 ataaccaagg caaaaccact catgataccg gtcactatga ttgtgagtaa aactgtgaac
     4081 ttttttttga ttatcaccac tctcccttac ttaaatgatt ctttgataaa atcataccgt
     4141 ttttcatcaa aaaaacgtat ctatcaaatg tataggtcat gtagaataaa tctatatact
     4201 gtagaatcga aaggagaaga agatgaacat cggagaaaaa atacgaactt accgcagaaa
     4261 ttgtaagctt acgcaaaaag aattaggcga aaaaatcggt gtttccgata aaacaatctc
     4321 tagttgggaa aattctcgta caatgccaga ccttgaaatg ctgtcacttt tacatcagac
     4381 tttaggtctg ccgattgatt ttccttctat tgaatcagct accgaatcaa tagaaccaac
     4441 attgccggat gaacctcttg ttatcaagaa atcttattca tcaaaacaac ccttctcact
     4501 caaaaagaca ctatctttct ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa
     4561 ttatcaatat aaaaatgaaa cggttgatcg attcaatcct tttttgaaac cacaaatcgg
     4621 ctataccaca ctccctgtag gatacgtgga aaattccatc gaacattggg tatgtaatga
     4681 tatctttgtt catggtacat ggctgtcttt tcacgctgga gaggtagaaa aaggtaagaa
     4741 tcatgctgta atcaaacaca aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga
     4801 agccattccc tcagattacc aagaaatcct catccaagac tatgacgatg ccttaaaaag
     4861 aagccctcag gagccagata ggcattaagt aggagatgaa cgtagagata gaaaaaatca
     4921 atgcacatga tcttatcaaa aaaaaacagg ggtacaaatt aattgaaaaa gctttgagat
     4981 gaaagttcct aaataaaaaa gttcagaatg ataggtaaaa aaatgactaa ctattctagt
     5041 attaggctat ttgtattatt cttctgttat ttttatatca aaaaatccag aaataactaa
     5101 atataggtat ttctggctta tagtataata tttacgccac agcccccaat gattcttttt
     5161 ggaatcatga tcaaggacgg acatacacaa aaatattttg cccattcgat ccgaatagat
     5221 taacgactaa tctagtaagt aatatagatt ggtacgtagt caaaccagca aatggaaaag
     5281 ttgtctattc caccccaagg gctttaaaga cagcatcttc cggcgtttga taaaaaatca
     5341 aattaaaggt acccattaat tctgggggta cagttccaat atcagcagcg cttatcatag
     5401 gtacaagtac ttttttcgct ccggcatctt ttgctacttg taaacagtta gctaaattat
     5461 ctaccttaat catagaacca ccaattgtaa tatcccctaa aataactaaa cttggtaata
     5521 caggtttatt taaggaaact gagcataaag ctattatagt tgataacgat aatgtttttg
     5581 tcataccaat gccattcata tcttgaacat gaacgacaaa atcttttgtc gtcaaactaa
     5641 tagactgact aacacttcca taattagctc ttaaatatcg gtatgccgaa tccatcgctt
     5701 ctcttgctcc agcatctgaa ttgatcccag tacgttcaaa ctttccattt cccgaagtca
     5761 tttgcgtttc caatttatag gtacctaatt gcccagtttt gccataagat accgtgtaca
     5821 aatgccctgg cttccctaac ccttctggaa tcaaagttcc tccaccttgt tcaggaacag
     5881 aaacatactc ttctcgctct tcttctaaat caatgtatga aaaattgaca tcataaaatt
     5941 ccattccacc aattttcttt aattgttctt ttacacgacg acgcatttca agagcaaatc
     6001 ttagtatttc ttcgacatca cttttttcaa atctaccatc aggatacaac agtttcatca
     6061 tcccagatac aatccgacga actgcaatcg tatcccgctg atttaattga gaaccaagtt
     6121 tgaaatattt atcaaaacta tcgccaaaac taacttttcg aagctcacgc atgacttcag
     6181 aaaaataatc tgtaatgaag ccatagtcat ttgtaaaaaa atctggtcga tatttaggaa
     6241 tttcccaacc tggtaaataa caatgcatcc gatccaaaaa ggctgaatcc gtccccataa
     6301 cctccggaaa tggctcaaat aaattcgaag ttttcaatat tacatcaacg ctttgattaa
     6361 tatttcctac aaaagccatt gatgctgaag cattcttttc ttctttgcct ctagcaaaag
     6421 atcctgaagc catgtaatct ttcatgattt gaacgccatc attatcctta aatttaatcc
     6481 cagcaacttc gtcaaaagca acacagtccc acatacctac aagtcccatt gtacgactag
     6541 ccatattata gaaaaggttt gctactgttg tttgtccacc tgaaacaagg attgagtttg
     6601 gtgaaatttc tttatataca tgcgatttac ctgtaccacg tggtcctagt tcacagagat
     6661 taaaattatt ttcaattaat ggaataagtc ttgctaaatg aagccatttt acccttttat
     6721 taaattgtgt aggttccata ccaatggacc gtagaagtaa gtcaatccat tcatttgtag
     6781 taaactcctt gcgagccatc ttgtaagcct caatgtctaa accaggcatt tggattggat
     6841 caaccttttt tgcaataaaa gggctattcc aacgatcatt ctcatcatac caatattcca
     6901 tcgaaacaat acaccagatc cccccaccta ataatcgatc aaattttttc ggatactctt
     6961 cactaatttg aacatttttt aatcccaggt tagagaattc tgcttcataa caatctttct
     7021 tttcatttaa atgtacagtt actttatcaa taactgtata accacccaat tcacgaattt
     7081 ttgcttttac tttttctgct tcatcaggac gcacatagtt tcgagcaaga atatttttaa
     7141 cattttccac acctttttca accaatgttt catccaatga cgaagcatac ataccaagta
     7201 aatattccaa tacataaaca ggtacattcg caccttcttt aattttcttt gttaaatctt
     7261 ttctgacaat ctttccggga aagaattcag ataactttaa aatttcctgt tgttccacta
     7321 gaattcctcc atgtgttcag tcattataat agcaattaaa gtatatcata cttaaccagt
     7381 aggcttgata atacaaaaga aaaagtatcc acaaaaaatt gtggatacct cgtttttata
     7441 tatcaaaatc aaatgattct gcaaaaggat tatctataat gaattttatt cgctcggtca
     7501 aatcattttt ttgatttttt atcactaaat aataagtatc ttcaactgta tatcgacgat
     7561 ttacaaattc aaaaacaaat ttagtaaacc gctgatttgc atcttctgtt tttgaatccg
     7621 caacatagat ttcttcattt gagatacgtt caccatattc atcttcaaaa taaagccaga
     7681 aagatgcggg gtcataaaca tcactaattg catttttttg atagaattct aacgtggttg
     7741 ataatcctgt aattctgcgg ttgctagtca ttaattcaat tgctactggt tcctttgcat
     7801 tgctaccttt ggctaccgta atttctagaa cagggactag catttcttga atagatgacc
     7861 ctccatgaac gtagctttgt cccccgccag ctttcttaaa aatacttgaa gtaatcggga
     7921 cacaaatgtt tctagtgtca tcatttccta aagaatctcc caaagagtaa tttttcgtac
     7981 caattatttc gtattcttct tcactaagaa taaaacgacg ttctacacga tctttttcat
     8041 ttctagatgg attttcaatc ttatctactt cttcaacgtg gcttctttta taaataaatc
     8101 catgatcagc cgtaactata aaacgataga tactgccatc attatgcaag cgattaataa
     8161 gtcgggaaat ttcgcttatc gctaaatcac aggcctcaaa aactcgattt tcattcgagt
     8221 gatcacctgt tctatccact tgatcgtgat aaatataaat gactgatttg ccattaaata
     8281 cttctcgtac ctcagcctta ttcatcttaa ccacatcatc ataatttaaa gctacagcat
     8341 ttgcatctcg ttgctggaga atcgcatttc gattggataa accattcgtt tttttcccat
     8401 caatcagtac ttctgttcct tcattgtaag ttagttgatt gtttggtaat aaggcggctt
     8461 taccgaattc agtaactgaa ggtaatacag aaagaaagct agccatgttt gttgaatact
     8521 tcttttctct aactaactta cgttctaaat tcatccctaa ctcatatcga aaagcatctg
     8581 aaataataac tacagtttta acagagcgtt tcattacagc ttgattataa aattttcgaa
     8641 tggatggacg attcttaaat tcaaaagttt gattccaaat ttgtcctgat tgatcaagaa
     8701 acttcgaata atttgcttca actaatcgat acaattggcc atatttttgc ttttcttcta
     8761 tatgatcact attccaaata aatttccgat aatgcgtatc aacccaatac atttccttct
     8821 catattgagt tagcaaatta tccatgttca tcggcaagca caatctgttt ttatccacaa
     8881 gataataagc atgtttcaat aattgatact cataaatata atccgatgcg aaatgcattt
     8941 tttcgcgctg ctttaaacta tcaaaaattg tttgtcctgc aattttcgtt gtagtgtcag
     9001 aagcctttaa tcgttctgca taaaacatta atatttttgc atgaatctca gaaaatacgt
     9061 ctgcttcaat tagatcatca atttctatac tgtctaataa tccatcacca tcaatagcct
     9121 gatatacttt tttagatagc tgttcaaagc tttccatgta acgtgtatca ttcataaact
     9181 gatccatgaa agtaataatg tttgctttca aatgtagtat ttcaaatcct ttgaacttct
     9241 tcggcatctc ttgatcaatt tgataataaa aatagttcaa ataaagacac atcgtcagct
     9301 tttccaatgt tggctggcta tccgtaaagc caaatttcat ttgaataaat tcccaaaatt
     9361 tagctgttaa ctgatactta tcaagttccc ttaaaggatt ctccctactt tctgtaaagc
     9421 tttttagaac tacacgcagt aaatcattta atcgaatact tttcgatttc aacaaaacag
     9481 ccattatcat taattcaatc gtttctgatt tattaattgt catatgcgta ctaaattcac
     9541 caaaacttct catgcgatcc tttgcattaa agaaattacg atactgttta actgtctgat
     9601 gaaatctaag tggaatagcc aattcattca tgataatcgc taagcggtct gcactaaatt
     9661 cctctgaata attcaataca ctcaacaaat gattatcttc atctttatca tcccaacgag
     9721 gaaatggcgc atagattaaa tagttagttt cgggatcttc atattccaat aaacgcttag
     9781 ttttaaattg tgcgtccttt tctaatcgat agatttttgc attttgaatg accaatgagt
     9841 caatttcttc aagaaaatcc tgatttgaat caaaccaaaa aatgattttc ctacatccat
     9901 aattaggaaa acgttgattt aatttttctt caatttgttg cgtatttata tctgtcatca
     9961 atctcaccta atcgttctct ttctataccc cttatattga cataatttgg ataatttcgc
    10021 acgcaattta agaataaaaa agaaagttgt tagaaaacaa ctttcttaga taactatttt
    10081 tttggttctt tgaccccttt tgcaaaaatt tggaatttct cgccggtttt aggatcacgc
    10141 tgcaacttat cataattgac aattacacca tcatctaagt ctaattcaat tcgttttaat
    10201 gctaagtgac ccaacttctc gtcatactca cgaacttctt ttaattgctt ctgaatcttt
    10261 acaatttctt ttgtcattgc atttctttct tttgggttat cacttgttga aatttgttgc
    10321 tgacgcaatt cgattcgatt gtcatacgct ttttgtagtt cgtgtaggta ttcagtacgt
    10381 acttttccta attgatctgg tgtatagcga tgcaagtaca ttaacgcttt aaagccattt
    10441 tgttttcctg agtccaattg ccaataaatt ggtcgattgg agtatgtttt taaatggtct
    10501 ttgtaaaaat cttgtacgaa gtaacgacga ataatttctc gacttgtccc tttgccacct
    10561 aaagtgtcag cgataaactg gagattttct tcaagggttt ctttaccaaa agtaatctca
    10621 acgaaatcaa tgaaacgatt gacaatgtca atttggctat ccttgaaata ctcatcatcg
    10681 gtgatgagta aaacattatc agtatcaggt ttaaattttt gatagcggga aggatcccat
    10741 tcgccgccag cgaagatgag accatcttcg tctagggagt aacgaccgaa gatacaacct
    10801 attgaataag aaataaaatc cttaatttcc ctcgccttat caggtaacct tgcagagata
    10861 tattcatctt gaacctcttt tgttaattct ttttcaagag aataatgatt aatgaatatg
    10921 tcgttcagtt cctcttcatt cgattttaga gtattaaatt gattaaccaa ctgcctactt
    10981 agacagttaa atgcctctga gagatctttt ttaaacatat aacttaacaa aatgtttctt
    11041 ctaaactcgt atgacgtttc aagagcatta tagtcttttc tagctatatc gatattttca
    11101 tccacttttt ctcttatttt ttttaatttt ctctcagatg taactttaag aaaaggtagt
    11161 tttcccattg gtccttcatg gtaatccaag gtgggagaaa taaaagttaa aaagtgatta
    11221 actaatttag agtttacata gccaaatatt tcttcaaagt cagttatatc ctttgagaac
    11281 agaacagatc ctttagtttc aaaaattgcc tgattctgca atctaaaaga tacttgtcca
    11341 gatgaaagtg tcgaccaagt aattcccttt ttaaaatagt aattaagatt ttgtggtcta
    11401 gattttaact tcccattttc attaacataa ttttttattt catctccatc attctcccag
    11461 ttaaccacgt attcattgtt tccataccat tttctaaaac gaccaccttt agttgtagga
    11521 aaccattttt ttctagactt tttagcgtcc tctaatgttt tagcatcaaa ctttattttg
    11581 ttaaattcta cttcaaacca ataccgcaaa aatttattgt tatttccggt tgccaaccct
    11641 tgttttgcat ctgcaacatc gttagttaat tttccttgag caaaatcagt gattacgtta
    11701 tcgcttgccc agtaagcaat cggcattcct gggatttttt tgaagttgtc ttgattagta
    11761 ctataaaaat aatcgcattc accatgctga tgaattgctt ctaacgtttt aattttttga
    11821 acttccattc cacctttaaa atctgataat tttatatagc ttccatcttg ttcatttgaa
    11881 ttttttatcg taaacgtaca aattggaacg gttgcttctt caaaagctga atactccatt
    11941 tgaatcaaac tagaaatgtt tctttcatta attaaaaatg agcgtaactt ttcatagcta
    12001 gaaatgaaca tccaaacaaa tggcgtcata aaggaagcat atccattatc aatcagcatt
    12061 tggctattca atttaataaa cacagaaaat agatcttttt tgacatcaga atagttatca
    12121 tttacatatt tttttaattt cttatccatt ttattaagat aaggcggatt agtgactacc
    12181 gctacatatt tatcaactag catttcagcc acgtcaagaa tgtgcaagta tttttcttgt
    12241 atttccttca tcccataaag aatatccata ttcgtaaatt cgttgaattt tgatatatac
    12301 tctcttactt ttcctaagta ttctttagtt attgcctgat tatttttatc gactaactta
    12361 atgattgatc caagttcttt cccattttca aattgatcaa aaattaactc tttgtattca
    12421 ttccagcgtt cactatactt atcattgttc aattcactaa tacgctcttg atactcgtct
    12481 gtcactttag gagaatcaat aaactctctt aaattcggcg atagactttt tttcaaaaaa
    12541 agacgatcgt attgacgccc tttcatcata attgcaaaat aagacaattg atacgctctt
    12601 ttatcgattt caagaccata taaattgtat tcaacgattg cacgtgcagc atcacgctca
    12661 gaatatccta acgtaagata aatttccatc aatacatcga aggcgtaaac taaaatatgc
    12721 ccagacccca ttgcgttatc gataattttt agttcttctg gttttaaatt acttacagat
    12781 gttgcttgtt caaaagcata tttatctgga attaagtatt tcaacttatt tcttaattca
    12841 ctattgggat aatgatctaa ccaatacttt cctaaactat tgtcaaccat ataacgaacg
    12901 acccaatctg tggtaaatag ttgagttgct gccggaatat cttgtttttt gacaatccct
    12961 ttataaatat tcaccacttg attatgtggc tcactattat agtactgata caaccaccca
    13021 ataatctcta cctgtccacc agcttgaaca tagaaatcat cctctggaat acttaaaaga
    13081 tcagcgatta caccatcttt tttaatataa gaagctgtaa acaaaagttc tgtataatct
    13141 tctacttctt caaatagctc aggtaagtaa tcattcaatt gattacagat ttttataaaa
    13201 atcaattgat acagcttgtc cattgcttct gctgaattat ctgttttcca agttctgact
    13261 tgttgctgtt cattactaga taattcttca aagatatcgg catcataaat atctgtaata
    13321 atatctggct caattttccc acttatttca ctagataata cacgatacgt gtcaggtaaa
    13381 taatcattta cctccataaa ccgaatggca atcaatcggt taaaccacgt ataagcaacc
    13441 tcttcgatta actcttgaaa agcttctgaa taagaagtga cagaagcacg ttgttttagt
    13501 tctgcaatca atttgtttct ttgcgctatt tcttttcctt gaattcttat tcctgatttt
    13561 tcgatttctt gtaaattgct atcaattttc ttcacatctt caatttttga ttcatcaatt
    13621 cctaaacgtt ttgctttagt cattacttca tcaattaatt ttctacgggc attgaccgcg
    13681 aaattcttta tcgctttctt atccattttt ctttcccttc tatatgtaac ctttgaaaag
    13741 acaggcacct ttttatgcct gtcttttcac tagaattgaa tcgtgatttc tgtattttct
    13801 tgaagttgtt ccttcagttt ttgattaatt tcagctgtta actcatcaat atctgctgct
    13861 gacttaatag ttttaacatg aacgttaagt gttctcattg gtactgtttt ctgttgaata
    13921 gtaggtttta ctatcgctat ttcttcagct tttgctgcag agtctatttc atctttttca
    13981 gtttcacgaa tcaagtttgc tgcttcaatt tgtttacgtg cttgttcttc ttgacgtctg
    14041 cggttttctt cattatcgaa acgttgatta aagatcaaat actgttctgc tacgtttaat
    14101 gataagaaaa caagatcatc tatagtattc gcttgttcaa tttttgcttt cagttcttga
    14161 aattcttggt caactattgg tacaaattga tccttaaaat cttttccttc taataattta
    14221 aatgttgctt gtttttcttc cgttaattta tttaatattt cctgactttc caactctaac
    14281 agttgattat attgttcaat aaattcttga atcaactcat ttagtttcat catatctgaa
    14341 taaggatcag cctttttcaa aatggttttc atctcatgaa tcaattgatc aatttcttgg
    14401 ctagaagttt gacgccgact tttttcgatt aatctaattt tatcttgtgc attctcccag
    14461 atttttttct gagtactatt ttgcttgtgg aaatttttaa ttttatcata tgtttcagcc
    14521 cagtcgaata attcatcgcg atattttttg attgtataaa aaaagtcttc aacatctgac
    14581 gtttgaacaa ttacttcaag atattttttt atttgctcat attcataagc actagggtag
    14641 gtataagtgt tttgtaaatc aaaataagag cgtaaattgt ctaattgacc ttttaaatat
    14701 ttcttgaaat tttccattgt atgttcattg ttattttcat caacaatatt acgactttga
    14761 aacagttctc taccgatttc gtcaaccaat ttttttgctt ttggcgaaat caccgcgctt
    14821 ggttcaattt ttatcttttc actttctcgt cgtttttgaa taatcattac aatactattt
    14881 ggatcatcca tccgtgaaat ttgtacattg ttataaagta aatcaatttt accagacatg
    14941 aaaagtttag cgatgcacca ttccacatca agttctgtga atccatacgg ttgttttgtt
    15001 tcaaaacgat cacgaattgt tttcatcgta atcgttaggt gatttctctt ttgatcattg
    15061 ataaaattga aaatttcctg aactgcttgc tcatttgatg gatcttcaat aatttcattt
    15121 ttgctttctt tcactaaata taagatatct tctttttctt taggcgcatc aatttggttt
    15181 aatttatggt atacttcttc agccattttc tgaagtgctg attggaattt tgctttaaag
    15241 tcggagcctg tcagttgaat ttctgcaccg ttatagaaaa attttgcaga tttcaacagt
    15301 tcatttaatt gatcgttgat acttgcactt agttcctgta attcaccaga tttttccgaa
    15361 cgaatcgcgt caaagttctt tactacactc gaataagtcg ttgttaaata aagattcatt
    15421 ctagcataca ttgctatatc atctttaaat tctttatctg atggcaattc tacgaataat
    15481 aagttacggg aactcttaag cgcaagttct gcctcttgac ctgctaaatc agagtatggt
    15541 gaaataaaat taactgtcat ttcagcattt ccagcttgct tgtatggacg ttgatctaac
    15601 acttgtgcat aagtaaacgt ataccggttt ttaaatttat ttccacctga catgcctaat
    15661 ttttcatagg caaaactatt tcggtctaga acgccgtcaa aaattcgttt agatagttct
    15721 acgatcaaat cactatctgt aatattttga tttttaatct cacgagtaat ttcctgttct
    15781 tcatcagttt ggaaaatata actatctaag ttcttccgca caaagttttc cttaattaaa
    15841 aggtccaatg cttcttctac tcgtttttta gtttccaggc ggtcttcgtt gatatttctt
    15901 atcatcaagc tcgtaatatt ttcaacattt ggtttgatgg cttgatcaac atatttaatc
    15961 atgaataaaa ttcgtaagac atttacgtta aagttatcta tatcagttgg tctttgaata
    16021 tgagaattat ttgtagcttg tgtaatcact cggctaattg ctgtatcaat ccaacgttct
    16081 aacgagtaat aaaactgatc taaagaaacc aaactggttg tatcattttc cttatttcgt
    16141 tcagcggctt ctttaaacat tgctagtaat gaacgttcac cagacgataa gtttgatcct
    16201 tgatagctgt gattacgaat ctcggttaga atttgcccca ataaattgaa ttgataggga
    16261 ataaatggat aaacttctgc gtaattttca gcatcttgga agagctttaa ttcaggacta
    16321 tcaacaaaga gtaatttatt tttcaattct gtttgaacag tttcaaattg taaggctaaa
    16381 gaatcattgg caaactcttt tttcttcaaa atacgttctt taattacttc atcggcattt
    16441 gctgaagata aactcacagt tgtggcaaaa cgatccttga tcttagaaaa atcattatca
    16501 gaattgttat caaagtttgt tacgtgatca atcgcttcct gacttgtcac taatacccaa
    16561 gccttacctt cacaaattcg acccaaatct tccacaacgg tttgtaactg caaagtcaac
    16621 tcacgattac cgccgatata ttgtccgacc tcatcgacta gaaaggcaat atgatggtct
    16681 gcttctttgg aatcaagata gcttttaact agatttgcaa agtcatcaat gcttattaaa
    16741 taatttcctg atgctttttc taaaaaatta tcaatagcgg cttcactcat aaaatcgatt
    16801 ttaagtgccg cttctttgat atcatcttgg atccaatcaa actcatggcg ctcttcttgc
    16861 cattttgccc cattaatctc ttcaaaagca gctttgaatg catcaaactt ttcttcttta
    16921 attaaccgac gttcaaaatc tgctagatgt ggaatcgtgc ttaatccttg catttcgtta
    16981 aataccttta aaaaaacatt cacgattccg tttttgtctt ttttaccacc tgcatcactt
    17041 tttgaatcaa tattaaacag aataacatct gtatttattt gtgagcaccg ttccatcgtt
    17101 gccaaagtca gtggatcttt gattttccca tcttctttga agtaatcaat ggctttcttg
    17161 tcatttattt ctcgatttgc gagcaagtaa gaaataattt ttaagaaatg agatttacct
    17221 gaaccaaaga acccctgaat ccaaacaccc attttatctg ttggtgtatt gatactccgt
    17281 tggtaggcat caaaaattgt tttaaagtgt ttttgtagtt ctttcgtaac cacgtattct
    17341 tctaattctt gttgaacatt ttcatccttt gcttgaccga ccttaataac tccttgaata
    17401 tttcggtcaa ttggttttgc aaaaatatct ttaattagca tttaatctcc taccttccat
    17461 cccaactaaa ttctaaatgc tcgataataa ttatcatctt tattggtatt aagaattgtc
    17521 aaacttaatt catcatattc ccctggatac atcatcacaa ctggtacgcg atcaaatacc
    17581 tgatgcattt tatttaaaat agagtgcgct ctaattaatg gatataattc gccaattcct
    17641 gtcaggaaca caactgattt ttctggtgta tgggcatcca catattcaaa aaatacattt
    17701 tcttcctcag gtaaatccat tttcaataaa cgaccaatgc tttccactaa tttttgtaaa
    17761 ccacgtcgtt cttccatctt aatcgctttt tcagtgaaac cgcgtttttc tagatactct
    17821 aacataatca catagagatt ataaactttc acttcataat tatcaccaac agatgttcca
    17881 gatatatcat taatataatc tcttacaatt aactcgtctt tcggatcgta gtcaaagata
    17941 taatagccta cttcattacc taaaccacga ttcaataaaa aatcttcttt ttgaattact
    18001 tcattcaact tttccaaatg ctcttgaatc gataatgaca ttataaaatt cctcccagtg
    18061 ctagtacaat atctttacga ttcttgtcaa ctaaccattg aatcaatcgt ttatctaata
    18121 atatcggagt cattcgtcca tattcttttt cccctctgac taatcctgtt tcaattagat
    18181 tcgtttgata agcggcaata agccgtttaa tcgtataatc tttccatttt gaaatatctt
    18241 ctgaacttct ccgcttctct gtaaaaaaag aacggtaatc aactggtgtt aattgcaaat
    18301 ttcctttacg tagattttca gcatatacct cagtcataaa ttcattaaaa agatcatcaa
    18361 tcataaggat ggaaatcaaa ttcactaact tttgattatc tatatttaac tgattgaaaa
    18421 gttgcaatcc agcttcatcc agttgactaa ctctcctttt cacgttctga tagatttctt
    18481 tttgacggtt actgctcact gcaaaaaaaa tattttcatg ttcatttttc tctttaattt
    18541 ctatatctgt atatccttct agtcgtaatt gaatatataa cttgaactct gaaaaccaaa
    18601 agcgtttaga aactagacca gcagaatatt ttttattcaa tagattgcct cctaatcatc
    18661 tataattcaa taagtaaaca agtgtttttt gctcatcgtt agcattcatt ttttagtatc
    18721 tactatacaa tacatattta ttata
//
LOCUS       DAZKCC010000059        16393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000059 DAZKCC010000000
VERSION     DAZKCC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.060"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..423)
                     /locus_tag="ACG8VH_002031"
     CDS             complement(25..423)
                     /locus_tag="ACG8VH_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9110578.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(420..1193)
                     /locus_tag="ACG8VH_002032"
     CDS             complement(420..1193)
                     /locus_tag="ACG8VH_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9110579.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1190..1873)
                     /locus_tag="ACG8VH_002033"
     CDS             complement(1190..1873)
                     /locus_tag="ACG8VH_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9110580.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2040..2927)
                     /locus_tag="ACG8VH_002034"
     CDS             complement(2040..2927)
                     /locus_tag="ACG8VH_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9110581.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2924..3793)
                     /locus_tag="ACG8VH_002035"
     CDS             complement(2924..3793)
                     /locus_tag="ACG8VH_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9110582.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(3930..5216)
                     /locus_tag="ACG8VH_002036"
     CDS             complement(3930..5216)
                     /locus_tag="ACG8VH_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9110583.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5489..6067
                     /locus_tag="ACG8VH_002037"
     CDS             5489..6067
                     /locus_tag="ACG8VH_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9110584.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6067..6996
                     /locus_tag="ACG8VH_002038"
     CDS             6067..6996
                     /locus_tag="ACG8VH_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110585.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            6993..8729
                     /locus_tag="ACG8VH_002039"
     CDS             6993..8729
                     /locus_tag="ACG8VH_002039"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9110586.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8792..9628)
                     /gene="pheA"
                     /locus_tag="ACG8VH_002040"
     CDS             complement(8792..9628)
                     /gene="pheA"
                     /locus_tag="ACG8VH_002040"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP9110587.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9625..10134)
                     /locus_tag="ACG8VH_002041"
     CDS             complement(9625..10134)
                     /locus_tag="ACG8VH_002041"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP9110588.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10127..11422)
                     /gene="aroA"
                     /locus_tag="ACG8VH_002042"
     CDS             complement(10127..11422)
                     /gene="aroA"
                     /locus_tag="ACG8VH_002042"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9110589.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11435..12526)
                     /locus_tag="ACG8VH_002043"
     CDS             complement(11435..12526)
                     /locus_tag="ACG8VH_002043"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP9110590.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12543..13709)
                     /gene="aroC"
                     /locus_tag="ACG8VH_002044"
     CDS             complement(12543..13709)
                     /gene="aroC"
                     /locus_tag="ACG8VH_002044"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9110591.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13711..14775)
                     /gene="aroB"
                     /locus_tag="ACG8VH_002045"
     CDS             complement(13711..14775)
                     /gene="aroB"
                     /locus_tag="ACG8VH_002045"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9110592.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14808..15830)
                     /gene="aroF"
                     /locus_tag="ACG8VH_002046"
     CDS             complement(14808..15830)
                     /gene="aroF"
                     /locus_tag="ACG8VH_002046"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP9110593.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4732 a         3617 c         2909 g         5135 t
ORIGIN      
        1 tttatttcct cttcaaacgc ttgtctatga ttcagacttt tctctatcgt tattttcatc
       61 taaaagttct tgcattccga taatttctcg atattgatga atcttagtga taccaatgac
      121 tttgtctaca tcaatactaa aacaaatacc attacctgga atatcgatgt tgatgccttg
      181 accaatcgca tccattactt tattcgtcaa actatcgggt actaatgtca acacaatatc
      241 tttttctggc gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac cagaacctct
      301 tccatgtaag atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga taacatcttc
      361 gcccattcct ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta caagatcttt
      421 catttgcttt cccccaattt ttgcaatctt tctttttcga ttctgtcttt ttcatctttt
      481 cgtttataaa tatatcctag tactaaagtg gataatattg gtgtcaatgc aatcattgca
      541 accacaccaa acccgtcaat gatcggatct cttccttcaa tttgtgcagc gattgccacg
      601 ctcattgata aaataaatgt cgaacacatc gggcccgttg ctactccacc gttatcaaaa
      661 gccattgcta aaaagatctt gtccactttt cgtcctaaaa taaatgctag cccgtatcct
      721 gggatcaaga aataataaag agagaatccg acgagcaaac gccacataga caatcctact
      781 gctgcgccga tcccaattga aattaccatc agcatgattt tcgatcggat tctcccctcg
      841 ctcaattctt caacctgttt gatcattaca tgaatcgccg gctcagcaaa ccctactaag
      901 aaccccatta aaaagccaag gggaaccagt atataattgt aaggcaatcc cgcaatagca
      961 ctgcctaaat gctgtcctac cggtacataa gccatattga ctccgtgcag gaaaaggatc
     1021 aacccaatag tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt tttgcgcata
     1081 cgaaaactaa tgatattcat taaaacaaat aggattacga taggtgccac tgccaaaatg
     1141 acttccatgg taacgatttc gattccatct atcacttgct gccagaaact cattggaaga
     1201 tcacccccaa aatcatcacg gcaagaatcg gccctaacga agcaataccg accattccaa
     1261 aactgtcatt ttcatttttc ttttgtttga tcatactagt catccctccg gcaagggcca
     1321 agataaatgg aacagttact ggaccagtag tcactccccc cgaatcaaaa gcaatcggca
     1381 taaattcgga actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct atagccatca
     1441 attgataata ggatagcttg aatacgaccc gaagcagcgc aaaggctaga aatactccag
     1501 tgccgacgct gacgatccca atcagcaaca ctctgccaat ctctccgtca gatatatcat
     1561 atacttgttg tcccaaaact tgcacggaag gttcagcaat cgtgatcacg atcccgatag
     1621 caaatcccaa actgaccaaa acggtcaaac ttttcttttt gatcatatat ttccctacta
     1681 gatctccgac ttccatcata gaataatctg ctccaaacaa aaagagcgtc attccgatca
     1741 tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc tccttctaac ggtgcaaaaa
     1801 caaacgttaa aatgacaatc aggacagtca tcggcaatat agcagcaatc acttctttaa
     1861 aattttttag catgtatcct cctcctttac tctttaagtt taccttaaaa tgaaagagtt
     1921 ttcgagcaca aacttaaaac gttatcattt aatttttctc aacataaaat aacacacgtc
     1981 ccaaaagtat ctttttactt ggaaaaatga ctttgaagga acgcgcttat tgtgtttttt
     2041 tattttacag agccgatcat tccttgaacg aattgctttt gcaaaacaaa aaatacgatg
     2101 gcagttggta aagtagcgat gactaatcca gccatgatca ttccatagtc tggtgtatag
     2161 gaagcaccca tagcagataa aacaagcgga accgtccgtt tatcaggaga ttgcaaagct
     2221 actaagggcc ataaataatt gttccagcta ctcatgaatg tgatgattgc agccgcggca
     2281 taggtatttt tagccgtcgg catatacact ctaaaaaaaa tacttagttc ttttagaccg
     2341 tctaatcgag cggcttctac tagatctttt ggaaaagatt taacattttg tcgaaagaag
     2401 aagatcaaaa aagcagtgct aacagatggc aggatgacaa ccaagaaact gttgattccc
     2461 aaaaccgttc cgttcaattg tgaaaacatt cgatataaag ggatcatcaa tgccgcaaaa
     2521 ggaaccatca tcgatagcag tagaatattg aaaattaggt cttttcgttt actttgataa
     2581 atctcaaatc catatcctgc cattgacgaa atcagcaatg caaacacagt ggtcaccaaa
     2641 gcgatcatcg ctgaattcca aagggaacga gtaaaattca gctcattgga aaacaaattt
     2701 tgaaaattga tgatcaagtt actgccaatc tttatttttc ccgatgtgat atccacgggt
     2761 gtattcgtca ttcccaaaac catccagaca aaagggaaaa tggaaataaa tgataggatg
     2821 cttaatgctg tatactttaa tactgaccca attgtcgaac gaagttctaa tgcttgtttt
     2881 ttgacgttgg ggcgttcgat tgtttctttt tctaccatat ttttcatcga ctttctcctc
     2941 ctactttcgt ttggattatc gacaatatca caataaagga aacaataaca aaggaaactg
     3001 ctgccgcata tccaaaatta ggcgtgaatt tataactcaa attatagatg tattgagata
     3061 gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt ttgtacttca tcaaaaagct
     3121 gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat gattggtttt aaatttggga
     3181 tcgtcacttt gataaactgc tgccatttag aagcaccgtc aatagatgct gcttcataaa
     3241 tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat catattgtat ccagtccaac
     3301 gccaagtgat cgaaagaatg atcagaactc tcgcccaaat tggatgagtc aaccagccaa
     3361 tcggtgtgtc aatcatacca atgcccaaca acaagttgtt tacaaggcca gattgagaaa
     3421 atagactttt catgatcaga gaataactga ccatcgaagt gatacatgga agaaagatag
     3481 cggttcgaaa caacccttta tatttcaact ttttatcatt taaaaggttg gaaataaaaa
     3541 gtgctagaaa caacatgatc ggtacttgta caagtaaata caggaatgta ttaaaaaaag
     3601 cttttttaaa agttgcatct gtcaacatcc gctgataatt acctaacccg ttaaatgtca
     3661 tattgacagg gggaccactt tgaaaagacg tgatcaaagc caaaaccatc ggaataaaaa
     3721 ccatgattga tatcaaaatg actggcaaga ttaaaaaagc atgcccgttt ttgaataaat
     3781 ctttctgctt cattcttttc cctccaattc tatcattgat tatctacatg aaaagacaag
     3841 aaagacaata tctgtaaggc agacgtgatc ttttttgtct tttcgtttgt ctgatgaaaa
     3901 agcgaagaaa ttcacttttt taccaccgat caattagcca attgtgcttc tacttgtttt
     3961 tgtgtatctg ctagtacctt gtctgtctct tctccattga tgattcgatg aagagcttca
     4021 gctacgactg cttcaattgc ataggtctca tgtccgtaat tcacttctgg tatttcagat
     4081 gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt aaaattcaga agcttcttga
     4141 taagcttctt gttcattcgc gctaagcatc gtagatacta gtccgatttc tttcgcaaga
     4201 gtgcccatca gttcttcatt cgaagcaaac gttgcttcta ggaaatcttt cgctgaatct
     4261 tctccagcta ctcctttgat cacataccag ccagcgccgc caaggttaga agcttgcgct
     4321 ttttgcagat tttctggaag tgcaggtatt ggtgcaatct tccattttcc agattggttt
     4381 tctgctcctt ggatcgttga agaataccaa gcgccagttg ggctagaagc tactgctcct
     4441 gattgtatcg cagttactcc agcattccag tcagaggttt gttcgactag atcttctttc
     4501 aaaagagtgg caaacaattc aagaccatac tttaaagatg cattgtcttg gatcgtgact
     4561 gtctccccat cttcagatgt ataccattct cctgcctgtt gcataatcat ccttactcga
     4621 ccaaggtcag aaggattgac ttcagcgatt ttctttcccg tcttttcttt gacatctcgc
     4681 gctacttgaa tatactcgtc ccacttcaga ttctccatat cctcttctgt ataacctgct
     4741 tctgccatca gatccgtacg atagaagttt gctgttacgc ccgaatcgaa aggaacaccg
     4801 tatattttat ctcctacttt gtttaccgca aatttatacg gtgcaaaatg atcttccttt
     4861 acaatagcag acaaattagc gaatgcatct ggataagaag tcaaatagcc ttgtatccta
     4921 taatcttcaa tcaagacgat attgggcaat ccttctgtat tcccgctcgc taaagcggtg
     4981 ttcagtttct gaacgatatc atcctgagac atcgtaacaa cttctatctc tgtttcttcg
     5041 ttctcataaa ctttttttgc ttcattcact gctttgatat tgaatgtctc atcccacgcc
     5101 cagacagtga ctttttcttc tttgtctgct gtactactag cacttccgca tcctgttaag
     5161 aaacctgatg ctgccgctaa acaaacgact ccagcaatta actttctgat gttcattttt
     5221 atccgctcct tttccatcca ttagttgttt acgtttgcta cattcatcat accaaaattt
     5281 gaaaacggtt tctttattat tcagatactt caaaaatttc tcctaaaata ataaaaaaat
     5341 acaaatcaac tatgacaaaa ggaataatct tagcatctag accaaagatc gaaatggtga
     5401 ggtttcttta aaataaaaat atccttgcat tttctcctag aaataagtat ttttaaatta
     5461 caagactatt cttaaggcgg gtaaaaaaat gccaatacgc gtaattctta tcgatgatga
     5521 acagacgatt ttagatggaa tgaaacagtt gatcaattgg tctgagcacg ggtttcaatt
     5581 aatagggact ttttccagtc cgctgaaagc gcttgatttt tctttggtac atgcaatcga
     5641 tatcgtggtc acagatgtaa tgatgcctga attaaatgga atcgaattat cgcggctatt
     5701 aaaacagcaa caacctgaaa cacagatcct tatcctctct agctatgatg aatttgatat
     5761 ggttaaagat tcttttaaag aaggcgtcgc tgattatctt ttaaagcctc gattgagtcc
     5821 tgattttttt ctaaattcct taactgccgt ttcacaaaaa atcaaaaaaa agcccaacaa
     5881 aagcccaatc accaatcgtt atgagcagat ctgcgagaat ctttcacagc agattgctgg
     5941 acaaactcgt ccattgcttg accctcttct ttttgaacac accttgtttt tctgttatat
     6001 accgaaaaac ggccattcag ttcaaaacaa ggctctaaac aaaaacagtc gatcacccat
     6061 tcattgatgg atagcgacga ttcgtttact atttatcctg tttctacctt ttcggaagaa
     6121 tccggctatg taatcaatac tgattccaaa gatacactta ggaagtttgt tcaaaagttt
     6181 cgtcaagaag tttttccagg tgattttttt gttgtttctg attcttttgc aatccaagat
     6241 atcaatgaaa tattccaaga gatcagaaat tgcaccaaag gacaacgttt ctatcataag
     6301 ggaaagaacc ttgcttatca gcatgaactt cttccgttat taaccaacca cgagcagcaa
     6361 gcaagtagtt atttaacaaa aatcgttcat aaagactacg ctttagctgt caaagaactt
     6421 gagaatttct tcacgacgat attggaacag cctattgatc cagcttattt gaaacatgag
     6481 gcaatcaatc gtttctatgc attactcaac gctttggctg acgagcatgt tgggaatgaa
     6541 gaactttctt tgctcaagat cgcccttcct tctttattag ccgatgctga gtatttcgaa
     6601 gatttttcta ttcttgtcaa agaaacactt gagaaactca cccacttttc tgcaaatgca
     6661 aaaaaagaca atttcgatat cttgactttg atctacgagt tcgtccagga aaattatgag
     6721 aaagaaatca atttacaact tttatccgaa aaatatcatt tttcatatag ctatctctca
     6781 gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat atctaaaaaa agtccgtatc
     6841 agtaaagcca agctcttttt gacggaaaca cggcttactt tgacggaaat ctgtgagaaa
     6901 gtcggctata ccgaactggg ttatttctcc agagttttta aagaagagac aggaatgaca
     6961 ccttctcaat accgtaaagg aacttttgta aaatgagaaa aaaaatcaaa gagcatgaac
     7021 tctttgccaa acttctgttc attttgttca tcggtctttt tctgcaagcc atcgtcattt
     7081 ctttgtttat ttatcatcga tcaagggatg cctatatcca gctgttcaat caatcgaatg
     7141 atgtggtact aaaaaagatt caatctgaat ttgaaagttt gaatgataca atcgagaata
     7201 cattagccgc ttttgattct aatccagctg tcaaaagcta tttttccaat gatccagccc
     7261 aacatatgga acagcttcag caactaagaa caatccaaaa aatgaatgac tctctttcaa
     7321 aaatccatcc aatgatcgat tacgatgtat tgatctttgg agaaaatgga agaacttttg
     7381 tcggaaacga catgctcact gccgtatctg ccgattcttt ttttcaatct gcgattgctc
     7441 agagagtgaa tgaacgtgct gctgatacac aaatgctttt tgcacaccac ggattgactc
     7501 ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa actgaaaaat acattgaatc
     7561 acgtttacgg atatgctgtt ttatcgatct catccaagca actagcgaat ctttttcaat
     7621 ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa cgaagaacag acgatcatcg
     7681 catcaaatga agaaaataca attgggagcc gatcaatgat tttcaaaaaa ctaacagtag
     7741 gtgaaacaac gatttcaaat ggacagcagt tgacccgtct tcctctgtac cgccaggatt
     7801 gtacattact cagtcaagtc gatcttcatt ctttatctaa ccaaatgggc gtgatttttc
     7861 cgattatttt gtataatatc gtgacacttg cttttgctgg ctcattggta tttatgtatt
     7921 tggatcgaca cacaaaatcg atctaccgct tgattgattc gctaaaacgg ctagaggaag
     7981 atggcttaaa agctactgtt cctgtacaag gaagccatga agtccaattg ctagggaata
     8041 cgatcaatca attgctaagt aatgctcagc atcattatga acagacgatc caagacgaaa
     8101 agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca gatacagccc cattttattt
     8161 ataatacgct gactagcttg aaatttttga tttggcagaa aaaaacagat gaagcagttc
     8221 aaggaatcga cagtttcatt actttactaa gaagtacgat tgggaaaaaa gaagaagtga
     8281 ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat cgatcttttg agtatgcgat
     8341 atggcgaaag aatctcggct aaaataatga ttcctgatga actgcagtca ttatctatgc
     8401 cgaatatgat ccttcaacca attatcgaaa acgcctatct tcatgctttt cacaataaaa
     8461 cgcaaggatt catcacggtg tacggcaaaa caaaaaatca gcttctttta ttcgaagtga
     8521 ttgataacgg cgatggcttc gatactgaaa ataccaaaca aaaaaatgat catttttcag
     8581 gaatcggcat cagccatgta gatgagcgga tacgcctaat gtatggcgat gcatatggct
     8641 taacgatcca atcagtgatt gggacaggaa cgactgtaac gatttgtcta ccgcttcttc
     8701 ctaaaaatca atcagaatat caaaaataaa gaatagccaa gcgctgattt ttttatttta
     8761 agttaaataa atcaatcagt attcttgact ttcatctacg cgtctcttct actgtcagca
     8821 ctggataaca gcccaatatt tgactttctc ctccgagcat cttgatttct tggagcgcat
     8881 tctctactaa tgtcattgga cggtccaaca acaaatcgat cacgaaaaaa tattctccca
     8941 agctagtttt caatggtcgg gattcaatct tacttaagtt gatttcccgc cagccaaaag
     9001 ctgccagcat tttatgaagc atccctggcc gattagcagg tagtgtcaag atgacactca
     9061 tttttactgg agctgactgc tggctggtca tctttcggtc accgacgatc caaaaacgtg
     9121 tctggttcaa ctcattatct tggatattct cagctaagat ctccaatcca acatgctgtg
     9181 ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg atgttctgct acatacattg
     9241 ctgcagcagt tgttgattct gttgctacta aaggaacatt aggataatgc gtttctaaaa
     9301 actgttgtga ttgtgctagt gcttgtggat gtgaaaggat ttttgtaatc tttgttgctg
     9361 gattgcctaa aagctgctgc ttgataggaa gaacgatttc acttttaact tcgacttctg
     9421 gatgtttgga aagaagatcg attgtgtgat gaacagctcc ttccaatgaa ttctctacag
     9481 gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg caaacaaaca gggatggaag
     9541 catacgcagt caattgttct gtaggaaata gctgacaagc cgcctgataa gtgaatgaac
     9601 tttctggccc tagatagctg acgttcatat gcttttgatc ctttctataa tggtatcgat
     9661 gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt gtcgcacttt cttcatacca
     9721 agaaagtcgt tgtgccaata atgcttttat ttcttctctt gtttttccaa gagctaaagg
     9781 tcgaatagac gtttcatctt gttggatacg atcaactaga cagtccgctt ctgcctctaa
     9841 gtaaagaaca atcggctgtg tttttaaaaa acgccgattt tctgcacttt ggacaatccc
     9901 tccaccagtc gcgataattt tgtcttcatt tgacagcttt cttaaaatat ctgactccac
     9961 ttttcgaaag cttttttcac cgtaatagtg aaaatattcg gcaatcgaca attggatatg
    10021 ttcctcaatc tttgtatcta aatctacata ctctttttgt aaacattgag ctaacccttt
    10081 accgattgtt gtttttccag cgcccatgaa accaatcagc aaaatgctag ccatgagctt
    10141 ctcctcctga cacaagttct gctaaatcgt caaaaaatgc cggataagag attgaaacag
    10201 cttcagctcc ttctagttcg caaggtccat cagctatcaa agcagctact tgcagcatca
    10261 taccgattcg atgatcgcct ctgctggaca cttttgcgcc atgcagaggt gtcggcccat
    10321 ggatgatcag accgtctgct gtcgctttta tctttgctcc cattttctgt aattcttctg
    10381 ccaccgcatc aattctgttg gtttctttta cttttaattc ttctgcgtct ttgatgatcg
    10441 tgattccttc tgcttgagta gccactaaag ctagaatggg caattcatca atcagacgcg
    10501 gaatgatttc tccaccaatc gttgctttct tcaaatgact cgtcttgaca cttaaatcag
    10561 ccgattggtt ctgttcattg tgattcgtct gggtaatcgc tgcgcccatc tcctccaaca
    10621 catctaagat accagttctt gttggattga ccccgacatt ttttaacaac agttggcttt
    10681 ccggaacaag aagtcctgct gctagaaaga aagcagctga tgaaatatca cctggaacag
    10741 ttatctgttg accagttaat ttttgcggtc cggtcactat gatcgtttta tcttccactg
    10801 tgatccttcc gccaaattga cgaatcattt cttccgtatg gttacgggaa cgttcttttt
    10861 caacgattac ggatgtgcct tctgcttgta atgccgcaaa cagcaaagca gatttgactt
    10921 gcgcactggc tacaggcata ttgtatcgga taggtgataa agagcgggtt cctgtcacag
    10981 tgattggtag atactggtca ttttcttctc cttgcaagtc agcgcccatc tcgcgtaaag
    11041 gttccatcac tcgccccatt ggacgtttag acaacgaagc atccccgaac agagtagtcg
    11101 taaaaggctg tccagccaaa atacccatca ataaacgtat ggtcgtaccg gagtttccca
    11161 tgtcaattga ttcggttggt tggacaaatg agtggctacc gcgaccatga atcttgatca
    11221 cctcttcctc ttcttcgatc tcgacaccta attgttggaa ggcatgtaaa gtactcagac
    11281 aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc ttcagcaatt gctccaaaca
    11341 tgatgctgcg atgggaaatt gatttatctg ctggtatcct aacagtccct cttaatccat
    11401 gtatttgttg taataattgc atttatccag cctcctattt gatccagcaa tgataactgg
    11461 tattagacat aatgattctt tttgcttgta acaaatcctg ttggttttta aaactgattt
    11521 gcagtattcc taaaatatct tccctggtct cttggatctt caaattaatc aaagaaactc
    11581 ccgcttttcc gatcagtcca gttacttctg caataacgcc aggttcatct ggcacatcga
    11641 taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg gacaggcaac tggtctcgac
    11701 tttcttttgc ccgattgaaa aaagaaaaga tttccgattg gttctcagtc atgatccatt
    11761 ctgctatttg tgtcatttct tgttgccaat ctgccaatcg ttccaataat atctgcttat
    11821 tgctcaacaa aatatctgtc cacatgacag gatctgagga agcgatccta gtgatatctc
    11881 gaaagcctcc tgctgccagt tgtttggctc gcggatactc ttgattgaat tgttctgcct
    11941 gattgactaa gccagctgcc agaatatgtg gaaaatgact tagcattcct gttatacggt
    12001 catgttctct tgctgtaaga gttacatatt ttgctttggt tccgcaaaat agttcttgca
    12061 gtttttcaac atcttttttc gtcttttcca aaggtgtaaa gatgtaatag gcattttcaa
    12121 atagtttagc atctgctgcc tgtatacctg atttatgaga accagccatt ggatgtccac
    12181 cgacaaaccg aagatttttc tgctcagcgg atgtcacgac ttgctgcttc gtactgctga
    12241 catccgtaat gatgacgtga tcagacaagt tgattctttc aaggacatca agataagcta
    12301 aggttgtacg gacgggaacg gctaataaga taacgtctgc ttgttctgca cctgtttcaa
    12361 aatcacttgc aacgtaatca acgatctgct gttctactgc ttgctcacgg gtttttgact
    12421 gactatccca accaactact atcagttctg gattggtctg acgaatacaa agtgccatag
    12481 aagcaccaat cagtccaagc cccacgataa aaacatgttg tgccatctta tttgccccct
    12541 ttttagtaat gtttcaagta gtcacggtac tccttcactg cagctttcaa ctcaacaagc
    12601 gaatcacttg ggaattttga caatagctct gtcgctacca cagtggctac aacattttcc
    12661 gcaacgacgc tagctgcagg aactgctgta ctatccgaac gttctacact tgctttgtaa
    12721 ggttcttttg tatcgatatc aacactttga agtggcttgt acaatgtagg aatgggtttc
    12781 atgaccccgc gaatgacaag tggttcgcca ttggtcattc cgccttcgaa gccgcctaga
    12841 ttattggtac gtcggaaata gcctctttct tcttcccata ggatctcatc catcacttct
    12901 gagcctggta atgaaccaga agtgaatccg accccaaatt ctacgccttt aaaggcattg
    12961 atactaacca cagcttgtgc gattttggca tctagcttcg tgtcccattg tacgtagctt
    13021 cctaatccag ctggaacgcc tccaacgagc acttctacaa ttcctccgat cgtatctcca
    13081 tttttctttg tctgatcgat caaatctttt attctttgct caactgctgg atcaacaact
    13141 cgtacttctg acgcttcaga aatttctttg atttcttgaa tcgagatatt tccaggtatt
    13201 tctgcttgga ttccaccaag acaaatcacg tgtcctgcta cgatgatgtc aagttcttcc
    13261 agaattttct tggcaattgc gccgattgct acacgcatgg tcgtctctct tgcagaggac
    13321 cgttcaagca cattccgaag atctcgatga tggtatttca taccgcctac caaatccgcg
    13381 tgtcctggac gcggacgtgc aactcgccgc agtgattggt ctttttcagg cacttctgct
    13441 acagacatga cagttgtcca atttttccaa tctttatttt ctaccactaa agtgacaggt
    13501 gaaccaattg ttttgccgtg acggatgcca gacgtgatcc gaacacgatc tttttcgatc
    13561 ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag cgagttcgtg attgatttct
    13621 tcttctgaca gaggtaatcc tgctggtagt ccttcaataa tggctgttaa ttctggtcca
    13681 tgggattctc ccgcagtcat ataacgcatc ttacagtcct cctaagtaat ctttgaatgt
    13741 ctctgtggga acaggaacga tttttgcttg cccaatcttt tctagcaaga tgatattcaa
    13801 ttggcttcct cttgctttct tatcatgggt tatcgcgtgg aataattcat ctggatgact
    13861 tagatcgtac gtaatcggca gatggaattt ttgaagtatc ctttctaaag cagctgtcga
    13921 tccagttgga gacaagccga ttttttctgc atgagaagtg atggctacca ttccaatagc
    13981 tactccttct ccatgactga ctaccccata tcctgctgta ttttcaattg catggccaat
    14041 tgtatgacca aaatttaaaa taagtcgatt tccttggtca aagacatctt cttccactac
    14101 ttttcgtttg atttccaacg tagcagtaat gattggaaca gcatgttcca gaagatcttt
    14161 ttcgtctttc agctcgttca gctgtttcca aagcgttgca tctgctatag ctgctgattt
    14221 gatgatttca gcgattcctt ctcgtaaacg acgtaccggc aaagttttta gaacctcagg
    14281 atcaatcaat acaccagctg gctggctaaa tgttccgaca aggtttttcg ctttgtttgt
    14341 attgactgct gtcttcccgc caatactaga atctacttgt gcaagtagcg tagtcggaat
    14401 ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg aatgctgcta gatcacccac
    14461 cattcctcca ccaagtgcaa gtatcccatc gctgcgggta aaatcattat ctgctaaaaa
    14521 atcgtataac tcagctgctt gttctaaaga tttactggtc tcacccgccg gtacgattga
    14581 aaggactggt tcaaaattag cttgtctcaa ttggaacatg attttttctg catatagtgg
    14641 agcaacgttt gtatcagtga tcacagctac tttttgaggt ctccaaatcg tttgaagcca
    14701 tgatccagcc tgttctagtg cgcctttttc aatatgaata tcgtattttt ggttatcaac
    14761 atgtacagta agcattcgtt tcacttcttt cttttaaaaa gctggaatta atcgagtgtc
    14821 tgaatctttt ccattgcttc tttcatgatc gcaacttctt tcatcatgcg tgagtaagtc
    14881 ttttcattca acgattgggg accgtctgac caagcatttg caggatcagg atggatctct
    14941 acgatcatgc catctgctcc gcttgccaca cccgcacgag ccattggtgg tacaagttcc
    15001 caaattcctg taccatgact tggatcaaca atcacaggga aatgagttaa ttttttcatc
    15061 aatgggacag cacttaaatc aaacgtatta cgtgtcgctg tttcgtaagt ccgaatccca
    15121 cgttcgataa aaatgacagg agatttgtct tcgactgcaa tgtattctgc agcattaagc
    15181 cattcattga tcgtaccgga aattccacgt ttcaatccaa caggtttacc tgttttaccg
    15241 acagcagata gcagtttaaa attttgcata ttacgtgcgc caatctgtag gatatcgctg
    15301 tactcagcca ccatatcgat atgcccttca tccatcactt cagtaatcac tttcattcca
    15361 aattcatcag ccgcttgacg gatgtatttc aatccttctt cttctaaacc ttggaaagca
    15421 tatggagaag tacgtggttt gaaggcacca cctcgcagaa tttttgcgcc cccggctttg
    15481 gccattcgcg cacattctct gatttggtct aatccttcga tagaacaagg accagccatc
    15541 gtcacgaaac tgccgtcacc aatttttact ccgtctacat ccacgactgt attgccagga
    15601 tggaattctc gagaagtcaa tttgtatgtg tttaaaatac gcacagcatt ttctactcct
    15661 tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat caccttttag tccaatcact
    15721 actcgttctg ttccttgatc gattgagact ttgaatcctt ctttttcgat atgatcgacg
    15781 acttttttta tttgttcttc cgttgcttct tttttcataa ttactatcat tgtttttccc
    15841 gctttctttt gtttacttat ctatcttggc tgattttctc tactttttca aggtggtttt
    15901 acattaattc ctactaaatg accgtgtcct gttgttctca ttttcgctcc ttctttccta
    15961 cttatgaccc agataatcaa caaaaaaaga tgtccctgta ggaaattgcc tctacagtga
    16021 acatcttcaa cgagccttaa aaagcgctgc aaaaatataa aatagccact atagatgaat
    16081 ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc catcatagat acaaacctgg
    16141 tttgtttgta cccacgaagt catgaataca aacctatcta aaatagctaa agtaatacgt
    16201 attcagtttt gtaagttttc tagatttcat aaaaaatcgc tccaaactga tgtaagaatt
    16261 gtacctagta tagacataaa aaaatcgact gtcaatcatt tttatgaaac tttttttaat
    16321 tttctgatag ttaactttgt ttttcagaaa aataacaatt ggttttattg aagaaaatag
    16381 tcatgaacaa aag
//
LOCUS       DAZKCC010000060        16209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000060 DAZKCC010000000
VERSION     DAZKCC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.061"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1568)
                     /locus_tag="ACG8VH_002047"
     CDS             complement(<1..1568)
                     /locus_tag="ACG8VH_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFP9110594.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTI"
     gene            complement(1555..3243)
                     /locus_tag="ACG8VH_002048"
     CDS             complement(1555..3243)
                     /locus_tag="ACG8VH_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110595.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            3389..5026
                     /locus_tag="ACG8VH_002049"
     CDS             3389..5026
                     /locus_tag="ACG8VH_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110596.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(5171..5911)
                     /gene="truA"
                     /locus_tag="ACG8VH_002050"
     CDS             complement(5171..5911)
                     /gene="truA"
                     /locus_tag="ACG8VH_002050"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9110597.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6102..6737)
                     /locus_tag="ACG8VH_002051"
     CDS             complement(6102..6737)
                     /locus_tag="ACG8VH_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9110598.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6884..8104)
                     /gene="sstT"
                     /locus_tag="ACG8VH_002052"
     CDS             complement(6884..8104)
                     /gene="sstT"
                     /locus_tag="ACG8VH_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9110599.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            8323..9321
                     /locus_tag="ACG8VH_002053"
     CDS             8323..9321
                     /locus_tag="ACG8VH_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110600.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9517..11352
                     /locus_tag="ACG8VH_002054"
     CDS             9517..11352
                     /locus_tag="ACG8VH_002054"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9110601.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(11412..12128)
                     /locus_tag="ACG8VH_002055"
     CDS             complement(11412..12128)
                     /locus_tag="ACG8VH_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9110602.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(12355..12897)
                     /locus_tag="ACG8VH_002056"
     CDS             complement(12355..12897)
                     /locus_tag="ACG8VH_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9110603.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12902..13129)
                     /locus_tag="ACG8VH_002057"
     CDS             complement(12902..13129)
                     /locus_tag="ACG8VH_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP9110604.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(13208..15088)
                     /locus_tag="ACG8VH_002058"
     CDS             complement(13208..15088)
                     /locus_tag="ACG8VH_002058"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9110605.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(15277..15891)
                     /locus_tag="ACG8VH_002059"
     CDS             complement(15277..15891)
                     /locus_tag="ACG8VH_002059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9110606.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            16033..>16209
                     /locus_tag="ACG8VH_002060"
     CDS             16033..>16209
                     /locus_tag="ACG8VH_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9110607.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         5161 a         3065 c         2528 g         5455 t
ORIGIN      
        1 ataatagtcg ttactgtata acttgtgctt actttaatat cagcaactcc tttgactccg
       61 cttatttgta ccaatccatt agcattcacc tgctccttta caggttgatg atttatttct
      121 acattcgttc ctaaataagc atatacagga agtgtgatta cttggctctt ctcattagtt
      181 actctatata tgatagaact accagttgtt ttataaggaa ggtttaccca ttgactgcca
      241 atctttgctt cgtgttttat tatatgctcc ttattctcca atgaagcgat aggagaataa
      301 tcatataaaa aataatccat tcctttagtg gtatacgcct ctactgtttc tttgtttaca
      361 acaatatgtc ccggctgaga aattattttt tgattgaaat tttttttact agaaacagtt
      421 aaagtgatta ataacaaaac agtcataaat aaataaaata gttctcttgt cttattgtat
      481 cttctatttt tgaaaaatat catcgttaat atcagagaag agaatattat ttgcaagctt
      541 aagatccgcc aaggaaattg taacaatttg gctggcgtat tttgcagaag ataccatggc
      601 accaaactag ttgtagctag ccaagtcaaa cacgtaccat aataaatctt tttttctatg
      661 tttgttaatt ttttaaataa aatcagggga atacataaag ataacagacc aactagtccc
      721 attgagtaag tagttaagtc gttgttcata gcttcagtca gactatcgaa cacattagat
      781 gcatgagcat ataaatttgt cacgctaggt ttgttgattt tttggtagag catttgttca
      841 aacatcggat accaaaaagc actcgtcaga accaatgtca ttcctgccgc actaaataat
      901 tttttaaata tagcaataaa atctgcattc ttgaaacctt tagaaaccaa acgaataaaa
      961 ataaaaatga taattaatgc tgtactcatt aatgttgata gtacatggct gtacactagc
     1021 aaactcatac tcaatgctag ctttacagac ggatactttt cttcttttcc agagatgatt
     1081 tgataagcat aaaataatac agggataaca agtgttattg cgatagcttc ggcaattgcg
     1141 gaacgatagt agatgtctac tgtccgataa gcagagaaaa tatagagaca actaaataaa
     1201 aatgccgctt ttttttgttt taaaaaccgc tcgccataat gataagctac gagtgttgta
     1261 cacaagttca ataaccaaac gtaaataata tagcttacaa tcaaattttt tgtcatccaa
     1321 tataacatta cagctggaaa aaaagtcaga aagggataaa aataatttac accatatcca
     1381 gtataattga atgtctcata attaatcgga ctggtaaaaa tagtcgataa ccccttgatt
     1441 cgagataagt gaaaagaaag atctccagtg gtataaatac tctgtgtatt tttgaaaaag
     1501 atgaaagaat atatactact aactataatt aatccactca atattactac ttttttattt
     1561 attttcattc ttcttgttta tctccttttt agtcacatat cgagaatctt tcaacttatt
     1621 aggtgcaaat cgtttaaaac ttgaatacac cactaaacaa taagtcagaa tactgatcca
     1681 taaagccatg agagcagttg gagtatattt tgttgtaatt tctatcttat tttctccagc
     1741 atttacatcc acattcacta agccatgttt tttataagag tcatttacga ctttaccgtt
     1801 tacttttacc tgtgtaccta aataagcata tactggtact gtcacttttt gctttttgtt
     1861 gcttgccact gtgtaagtaa tgttattatc tgcagcttta tacttccctt tttcccacga
     1921 ttctcctact tttacttcat gtttttctaa gtgaggcctt tcttttatcg cttctgttgg
     1981 ggcgtaatcg tataatcctc ccatatctga tgttgtataa cgtgggagtg tttcttcatt
     2041 gataacaata tgaccgtgtt ttgattggat aacttttgcg taattttctt ttgctgctat
     2101 agacgtgacg agtagaaaca ccacagtagc gccaatgatt aaccatgaat attttttatt
     2161 catagtcttt ttcataaaaa caagcgttat gatcattgaa gaaaaaaaga tttggatacc
     2221 taagatacgc caaggaaact ggagtaagct tatcggtgtt ttttgaaaca aaaaccatgg
     2281 aaataaatta gttacgcata cccatgtaga aactacgcca acatagatga ttttttcttt
     2341 tttctccatt tctttcagta tgaataaagg aaaaatcaac gcaaccaatc ctaataaccc
     2401 catagtgtat gttgtaatat cgttatttaa cgcttgaatg atactatctg aaatagaaag
     2461 tgcttctagc tgcaaatctg ttttgaaggg actgtgtatt tcttgataat tcatctgctg
     2521 caacatagga taccaaaaat agctagttaa tgcacaagtc aaaaaaacag cctttactaa
     2581 attcacgacc acaaatttta tatcgttttt tatttgcttg cttaaaaaca aacgaactat
     2641 aatcatacaa gcaatcaatg cactacacaa tagtgtagtt aagacatgag tatacacaag
     2701 tagacttata ctgacagata aagcgatgac ttttttttct ctattgaata tcaatccata
     2761 agcataatat aaaacagctg ggataatcgt aagagcgatt gcttctgcaa ttgcggatcg
     2821 ataatagata tcaactgttc gataagttgc aaaagtatat aaacaactaa ataaaaaagc
     2881 agcatctatc tttttaaaaa acttttctcc ataataaaaa ctaatcagaa tcgtacaaat
     2941 atttaataac caaatatata gaacataact taatattaag ttatttgaaa ttccataaaa
     3001 aacgactgca ggaattattg taagaaaagg ataaaaatag ttcagtccat ctccataatt
     3061 gttaaatgtt gtatagttga ttggcccttc aaaaatagaa gataaccctt tgattcgtga
     3121 aagatgaaaa gataaatcac ctgttgtata aatttcttga gcatttctca taaaatttac
     3181 tgaatataaa gtactaagta agataagaac tactaaaaga aatgcttttt tacttatttt
     3241 catagatttg ttgttctcct tttttaattc tattttcagt atactagaat tgtctttttt
     3301 taataagttt tgcgttaaat ttttcatagc actagtatga tatactagaa aagaatcaat
     3361 ttttattact tttaaatagg agaaaaaaat gaaaaaaaac aaaaaccaaa taatagatat
     3421 catttttatg ttatttctaa gttttgctta cgtcatccct ctattcatgt ctaagggaat
     3481 ctatcatagt gtcaatcagg atacttactt tcatttatct cgaatcatag gactagataa
     3541 tgtatggagt tctccagtaa acttcaataa cttcgatcat catggaacaa tgatgaatat
     3601 tttttatcca tggcttactc tttatccagc ttttttattt tataaaatga tgggaaatct
     3661 tgttttaagt tataatatct actatttttt tatcactttt ttaacaatgg tcgtttctta
     3721 tttttctatg aaacaaataa aaaacaatcg ttatatttcg ctgttatttt cgattattta
     3781 tacattttcc gcctatcgtg ccattgacat attccatcgt gcttcattgg gagaagctgt
     3841 cgctttaaca tttttgcctt tgatactaat ggggtgttac gaaatttata ttagagatta
     3901 ccaaaaatgg tactggttgt ctattggcat gacgctagta gtgtatactc atttactttc
     3961 tgtcgctatg gtttctgtat ttattggagg aacgttgttt ctttcttttt atttttggga
     4021 ccaaaaaatc gctagactct taagtctttt aaaagctacc gtcctcacct tttttttaag
     4081 tgcgggattc ttaattcctt ttattcaaca atctagagca caagaattga aagtcccact
     4141 gggaaaagaa ttaagtggta tggctcctag tgatatgctg acacatattt taaataataa
     4201 ctataacaac tatactattg gattattctt atttctcgga ttgattggtg cttttatttt
     4261 tataaaaaaa ttaactgctg atgatttatt tattttcttt cttggtgtat ttgttctttt
     4321 ttgttcaacc aatttatttc cttggcaact ttttaaccat acaccagtta agagtctaca
     4381 atttgtctgg cggttaaatg gtttttcttc tttatttata gcctatacga tgagtatagt
     4441 gatctattat attttttcta ctaaaaataa ttcatggaaa ataatggtct ttacgtttct
     4501 tgcactaata ttgcatctct caggagtaag caattctatc catgctaaca aagattcatt
     4561 aacacttata gcacctgaag acgctgttgc tatagctgaa aattacaatc atactgatta
     4621 tgcaaacaaa gagtcaatct atcatcctga tatgattaat aatgatcaat atttactagg
     4681 taaccaggaa ataaatccaa caactcattt tatgagtaac aaacttacaa ttgaattaga
     4741 taattcgaat aataaaaata ctgtcttgac gacacctatt tatcgttata aagggcaagt
     4801 ggcttctatt aatggcaaac tagtacaaac aaaattatct aagtttggca caactgaatt
     4861 aactattcct ccaggtataa ataaagtcgt tattacttat cagtacacaa aactagcaat
     4921 tgcttcaaga tatttatcta tcgttacttt gattttattt ttactttatc gatttacttt
     4981 ttctaaacaa aaacaaccga gaagagaagt acaacattct cactagattt ttatttaaga
     5041 aagacttttt agctgtacac ataaacaggc taaaaagtct ttttttatat ttctttaatt
     5101 tgcttcatac attatttaac atcgatattt atactgtgaa acatgcgttt catccattat
     5161 caaagtattt ttatcgataa taaacttcat ccaaaaacag accttgtgca gggacggttt
     5221 ctcctgcatg ttgccgcact ttgctttcaa atatttcatc gattgtttct atcggcatgg
     5281 cacctgtgcc gatttctagt aatgtcccca tgataatccg caccatttta tataagaatc
     5341 cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat aattcatcaa
     5401 tcgtacgcac tgtggatttt tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg
     5461 tacccactaa tttttcgcaa gctgcattca gcttgtccat atccaactct tgagggaaat
     5521 aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt gttccaaaca taatagctgt
     5581 attttttgcc caccgcatta tatcttgaat ggaagcgctc aggcatttct tcgattcttt
     5641 tgatgacaat atcccgcgga aggtaacgat tcataaagtc gatcatttct gccagtccaa
     5701 tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca gcatctgttc
     5761 ttcctgatcc aataatttct atctcttcat ttgtcatctg atgcaatact ttttcgattt
     5821 ttccttgaat ggttttatcg tcatctccaa gtctttgcca tcctgaatat ctttttccgt
     5881 catattcgat tgtcaacttg atgtttctca tattttagtg gctcctctgt cttccatttg
     5941 gtcttctctc taccttttta agattgtagc acaaaatata gctccttgct tctctaagac
     6001 agaagctata caagaaaact gtggaaatat atatttcaga aaaacaaaaa acagccaccg
     6061 gatgaagtat ccgctgactg ttcaatttga cggctcaagt tttactgttt gagatgcaag
     6121 atactcaacc aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaaacct
     6181 attttattca gtaatggcca agaatttata gaaggaattc ttgccagtcc ataaatcgcc
     6241 gcaaaaagca aactgatcac agacagtcca aggtataagt ggcttacgtc catttgccca
     6301 ttttttcgat agatcaagaa aagaagaact ggaaacaata aaaaaccact gtaacctaaa
     6361 cctgatccga tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata
     6421 ctgaacaaac ctgtaaagat acaatcacct ataccataga acgcgatggc tgctgtaact
     6481 agggtagcta acacacgaga tgtactaaca aatgcagcat acaatgcagg caatgacagg
     6541 atgaaaaaga tccctgaaac cacagaccaa ataagaaacg cttttcttac aggactggaa
     6601 acctctccaa atacactgat cacgctcttc atttgatcta attcaggata atacaatcct
     6661 aatacgtacg gagtcaaaaa atcactcagc acgcctaata ataaaaagaa aaaaccaaat
     6721 ttacgtaaaa aatccatgat acgcctccaa taacaattac ctatgctttt aattgttatt
     6781 ataaacgact ttatcttgat ttaacagaaa cctctattta cacgaacatc tatataactc
     6841 attaaaaact cgaatcattt taaaaatact aaatatctaa cgttcaacgt tcacttaatc
     6901 catctaaaca atctttcaat ttgatttttt ccccacttct tttacgcgcg cctagctctc
     6961 ctattgcagt aaacaacaga tcacttgacg aatttaaggc agtttccatt gaatcctgga
     7021 taacaccgat aataaatcca ataccgacga tttgcatcgc gacatcatta gagataccaa
     7081 acaaactaca tgctaatgga atcaataaca gtgaaccgcc cgcaacacct gaagctccac
     7141 aagcagctaa agcagacaaa atacataaaa ggactttcaa aacaaaaggt gcttcaatcc
     7201 ctaaagaatg tacagcagtc aacgtcatca aggaaatggt aatcgcggca ccgcccatat
     7261 tgatcgtagc acctaacggg atcgatatgg aataagactc ttttgtcaat ttcaatcttt
     7321 cagccaacat catgtttatc gggatatttg cggcagaact tcgggtaaag aaagcgggaa
     7381 gtgcgctttc tttcaaacaa aagaaaatca atggataggg attttcttta ataaacaaaa
     7441 acgtgaggaa tgggtacaca actaatgcga caaacagcat cgtaccgatc aaaacaagta
     7501 aaagctgcag atactctgcc aatccagcga ttccagtcgt agcaattgat tgataaacca
     7561 atcctaaaat ccctatcgga gcaaatgcaa taatcacttg tacgacttgt gacaacgcta
     7621 tagatatctg atccaccaac tcttttgtcg attctgaacg tgctcgaaaa gctagtccca
     7681 acaatacagc ccagaataag acgccaaggt aattcgcatc aataagagat tgaacaggat
     7741 tttgtgtgac attcgtcaac atagtagata aaacactttc caaactttgc ggtgcttttt
     7801 cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca ataactgcta
     7861 caagtgcaga aagcaatgtt cccactaaat ataagatcaa aataggcttc acgaatgtct
     7921 tattcccaat tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg
     7981 cgattgcctt caagcaactg acaaacaacg ttcccaatat tgaaataaat gtccatgtgg
     8041 ggaaaaagat accaaacaat attccgacta aaataccaac aacaatacgt tgaactaacg
     8101 acatatccaa aaacttctct aacaaatgac caccctttca caattttaac aatcattgta
     8161 tcattttatg caaagaaact aaaggtataa ttttctaaaa attctaacaa tacaaacaac
     8221 catttcttgc taattttgta ctttttctgt ctacacgttg ttttttgaga tattctaagt
     8281 taggatgtta ttaatgggca acttatcagg aaacgttttc ttatgttttt gtccaatttt
     8341 tttgtgaggt gtttattctt gaaaaaaata aagaaggaga gttatggctt cctgacaatc
     8401 tttgtcgctt gtataatgat ggttttacag cgtatattga tttcagcagt cactattcct
     8461 acatatgcaa caaaccctgg tcgattctac atctatacat ttttacaaac ttccgttgtt
     8521 attggtgcaa atttgatccc actttacatg ggttaccaat atcaaaaaat cgcccgttta
     8581 aaagttttac attatttaag tcgttttgcc tttatattcc taatagccac actagtttgc
     8641 aatatctttt tttacgtaca agctggcagc ttgaatattc gtgattattg gcttatccta
     8701 acacctatca gtcagaatta ctttacttat gctgtttctt gtactttgct tttttgtagt
     8761 attccttata cagttggatt tttaaataaa ttatcgaaaa caacaattaa atatctacta
     8821 ttatttttaa caattggatt agttggctgc tctactctat ttaataaaga cccgtttgca
     8881 ttacagaatg gacagagtat tttatggatt ttttatttat ttttaatggg ttatggtttg
     8941 aaagaatgga actggaaaga aaaggtccgc tataagcttc ctcaagtatt tatcagcttg
     9001 attgttttat tcggtttgat tattttgatg acacaaattt cactcatcat cagaggaaat
     9061 acagatacag cgctgaggtt ttctaaacca tattctctct tcgcaatgta ttatactttc
     9121 tcttcgtttt tatttttaga aatgctatct gataaattag gtataaaagg aagaggaaat
     9181 tttcttgctg tatttttgat tgtcactcaa gttatatgta atttcccttt agtttcttat
     9241 cgcgtatcta catttatgaa acgtgaattt ccaaactcag ggaaggcatg gctgataaat
     9301 atcgggcagt tttttatttg atatttatta gcagtgtccc tcctagttat ctttttattt
     9361 tatatacaaa aaatccgttt cattcatcgt ttcatcgaat acttcgcttt tgattcgatt
     9421 gaacaattaa ctcagcgttg gaaaaaaaga attcattggc tttttgttca taaaagttat
     9481 tttttagttg cagcattttt ttactgcttt accttcgtgc agattttcgt tttagaacca
     9541 aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa tatttactca gcgacaagca
     9601 cccatgatcc tgacaatcat cattattatg ggatttttct tcttactatt gttgatttcc
     9661 aatcgttttt ggtacgcctt gactgctacc ttgatcatta acttactgct cactatttcg
     9721 acagtaatca aaatggaaat gcgggaagag cctgtctttc cttcagattt aaaaatgctg
     9781 accggattgt cagaactatt atcgatggtt agcccagtct tattaatcgt gggcggattg
     9841 atccttctcc tattattgat tactagtatt attgtacaac gtagactaca aaaacagtat
     9901 tccttgaaga ttcattggaa acgtcgattt atcagtatcg ctgtattatt aggatgtttt
     9961 tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt tcttgctgtt taatttattt
    10021 aaagtaaaca agactttctt taaccagcaa ggcgccgtaa aagataacgg accagtcatt
    10081 caattcctaa acaatatcga cattaaaatc atggaagaac ctgctggcta ttcgaaagaa
    10141 aaaattaatg gaataatgaa aaagtacaac acaaaggcag atgaaatcaa ccaaacaaga
    10201 agtgactggt taaacaatca gacgatcgtt ctcaatttaa gtgaaagctt cagtgatcct
    10261 gcacgtgtcc ctaatctgac agtacctaca gaccctattc cttacatcaa aagcattata
    10321 acagaaggaa caggaggatt gatgttatcc gtcggttatg gtggcggaac agctaacatg
    10381 gaatgggaag gactgacagg cttgagtatc agtaacctgt ctgcttcgtt agttacacct
    10441 tatacccagc tagtggagcg tcagaaaata agtccaaata ttaccgatct attcgatgaa
    10501 aaaatagcca tccatccttt tacagctgct ctttatcgca gaaaacaagt gtttgataaa
    10561 tttggttttg acaggttcta ttatttagac agtccggata aattaactta tacggataaa
    10621 atagaacaaa gcccacgcat atcggatgat tcagcataca aagaaacctt gaaagcttta
    10681 caaagcaata cggaaacgtc acaattcatc caattatcca ctatgcaaaa ccatatgcct
    10741 tatgatgatt actatagtga attgaactac actgctgaag gaactgctgt cattcctaat
    10801 agaagaaaag aattacaaac ttatatgcaa ggccttcact ataccgatac agcagtaaaa
    10861 aaattcatcc aagaaatcga caaaatcgat aaaccgatta cctttgtttt ttatggtgac
    10921 catttacccg caatctatag tgggaatgat tcgaaaaaat atggattaga acagcaccag
    10981 acagattatt tcatttataa caacaagtat agtcgtgaac aagcagctac gttaaataaa
    11041 gaagttgttg ctcctaataa ctttggtgct atggctcttg agcatgcgaa tatcaaagta
    11101 actccttact atgcgttgtt gacagaagta tctaatcatc tacctgcatt aacaattgat
    11161 cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg tctttgttac agaggatagt
    11221 caaattatcc aagaaaaaaa tttaactgag aaacaaaaag aaattctaga agattatcgt
    11281 ctgatacaat atgatcttgt tgcaggtaac cagtacagtg caacatgggc gacacaaaaa
    11341 ataaaagact aacaaataaa ctgagcaagg ggttgtgata aaagacatgt cacaacccct
    11401 tgtttatctc attaatttgc ttgtccataa acgatttgat taaagccatt tttcaattca
    11461 ttattcgctg tctcgttgaa gtaaagataa ttatcgggat tcccctgatt ttgttgtgtc
    11521 accaatactt ttggctgatt ttgctgaaac ccgaatagat agacgtgctt tttcagatat
    11581 ggttctgtct ctgcatcgga atagacggct gtgatttgat agtctgcttg acctgtccca
    11641 ctttctgacc attctgcaga aacaggcact ccaccaatag ccatcttcca ctccccgttc
    11701 aaaatagctt gagggacttt aagaccatat aagtccacac tcatttgatt cgtataactt
    11761 ttgtattgtt gtcccattgt ttggccccat ttggtcataa atgcctctaa ttgtgcggat
    11821 ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc tttctgaaga ggaagatgaa
    11881 gcctctacag aagaacttga agtagatgtt tgagttgttt cagtcgtttt ggaagactct
    11941 gacgaagtag aggtatctgc tgtttctgta cttgctgcag ttgacttgct ctctgtgcta
    12001 gttgttgtag tagttgcaac tgttgctttc gctgtttctt ttgttgattc ttttttcgat
    12061 tcattcccgt tggtacaacc agctaacaaa aaagctagac caattgttac taaaatcctt
    12121 tttttcaatt aaaaaactcc tttcgattac tcccacttct ttattctatc aaataagcaa
    12181 tgtgatttta ttgatttttc gtattttctc atcaatcttt caaatcttct aaaaactgag
    12241 aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc ttcttttatt ttaatcttcg
    12301 tgattttcca atttacctca cgctttatat ttgtatttga agaaacatag acttttagtc
    12361 atcatcgtct tcctcatcca aatcttgtcg gacagttttt ccttggagtc tttgtacttg
    12421 gtagatgatt tctttattga aacttaaaag ttcaacaagc aattgttgca agccatattt
    12481 ttcttctttt tcagctaatt tgattgcccg tgtgataaag acattttgga aatcaaaatc
    12541 ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat ttgttttgac catgttcgta
    12601 aatttttcca taacgcatga cttcttctaa agtagacgga acgatttcat tttcaatcac
    12661 taactcatga gagattttat tgaaccatct cgcttcttga tcagcatatt ctgaaaaaag
    12721 ggtacttaat tcttgcgaag atgtacccaa aacatacaat tgtgcttgga tcaatttcat
    12781 ttcttctaat ttaaaatttg ctaatacatg ccccaccatg gcgccagcag ttggtttatg
    12841 atggtcgata tctgcttgtt ttgtttcttg aacaaatttt tcttccacta tattcattga
    12901 tttacgcctc ttttactttt gctgattgga cttcataacc taatttttcc acaactgcag
    12961 ctaattgttc agggtggact tcatctggat caaagtttgc tttgattttg ctcgcattaa
    13021 acaaaacttt cgtattgctt accccaggct gttgagaaac cgcatgttcg attttttgca
    13081 tacagcttgg acaagctagt gtatctaatt gcataataat ttttttcatc gtagttcttc
    13141 cttccttttt cttgatagtc ttattctaga ttacagcttc cataaaaaaa ttgattctta
    13201 tcaagtttta cgatttttct tctttgcttt cgtgtccaaa atacaataaa cgcatcgcgt
    13261 taaagataac gatcaagata ctagcttcgt gaacgaacat cccactagcc atgtaaataa
    13321 acccggcaaa taagccgttg agtaaaaata agacgacacc aatggcaatc actacatttt
    13381 cacgggtatt taaaacagtt ttcttagcca agcggtaggc atgaaccaac gattcaaaat
    13441 tagattgcat caagacaacg tcactcgttt cgatagcaac atccgttcct gatcccatag
    13501 caatcccgat atcagccgca gccaaagagg gagaatcatt gattccatct ccaataaaag
    13561 caacgcgcaa tccttgttct tggtattttt tcacaaattg aaccttttcg tctggtaata
    13621 attcagcatg gacttcatca atgcctaaca tatcagctac ataatcagca gtcatctgat
    13681 tatcacctgt cagcatcact aactgttttg ctcctgcttg tttcaactta gctaattgtt
    13741 cagcgacttc tggtcgaatc acatctgaca cacctatgat ttgagtgatt tttttgtcga
    13801 ttgctacaat aatgaccgag ctgcctgttt gttgtaattg atgtaaatct tgtacttgct
    13861 ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt gttgcccgca caaactgtat
    13921 gaccatcaat ctcagctgtg attccttgtc ccttgattgt ctgattatct ttgactgcta
    13981 attgttgaaa atcaatgtct ttctcctgag cataatggat cacggcgcgc cccagaggat
    14041 gatcggataa cgtttctaat cgagccacta aagctaagct gtccccttca tcctctgtat
    14101 agttcttgat agtggataca gcagtagttc ctttggtcaa tgtaccagtt ttatcaaaga
    14161 caaaagtatc gattttggaa aaagtatcca ttgcttcacc gcctttgact aagatgcccc
    14221 gtttcgctcc attaccgatt cctgctacat tcgaaacagg cgcaccaatg actaaagcgc
    14281 caggacaacc aagaaccaaa acagtgattg ctaaacgaaa atcacgacta atcagaccaa
    14341 caaccaacgc aataagtaaa acggttggag tataatactg agaaaatcga tcaatgaatt
    14401 tttccgcatg ggatttggta tcttgtgctt cttctactaa ttcgataatt ttggcaaagg
    14461 tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta gccatctgct aaaatcgttc
    14521 cagagaagac ttcatctcca ctgattttga cgacttgtcg tgattcacca gtcactgcgg
    14581 cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc cacaggaata gctccaccag
    14641 tttttactaa gacgatatcg ccttcttcca cgtcatcgat ttccacttct tcagtcgttc
    14701 catcttcttg gacaacacta gcagtagatg gggccatctc ggtcaaagcc ttgattgact
    14761 cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc aaacaaaaac aagaatgtaa
    14821 cgatagctga ctccttgtat tctccgatca taaaggcacc aagcacagca atagtaacga
    14881 gtaaatcaat tgagataact ttgactttca ctgcctgata cgcatggatc ataataggta
    14941 tgaccgcaat aatagaagct atggctagag caatgttata gaacaagaca aaaccagtaa
    15001 attttccgat aaaaccaaaa ataatcaaca cagctgataa ggcggtgata tgatttcgat
    15061 gctcttggat ccacttttgt aatttcatct ttctccctct tttcctttca ttcttacgag
    15121 actaagtata gatacttatc catcataaaa aattgaccac tatcaagttt agtcaaaaag
    15181 gcggttttct tttggtagaa tacactacaa aaaatagtta gcaagaagac tcactaaaag
    15241 tgatcttgcc aactattgtc gtttctatcg aaattttcat tgaatgattt tcctgtacag
    15301 catacatcgg agatctattt ctttatctaa gatttttatc ttgctaattt tttcctttag
    15361 tctttcaaat ccacgtcgtt catagaattg atacgtacag ccactatctg taaaaaggaa
    15421 tatttctttt cctttctctc ttttttcaaa ttctgttagc aacttggtac cgatgccttt
    15481 tgttttcaaa tcaggatttg cagctaaaaa aatgatctct ccatctggcg aatgcttctt
    15541 taaatatgct gcaagcattt gttcatttgc tgtctcgtaa gctcctttac tttctttagc
    15601 aaatatattt tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa aagaacgatg
    15661 tttctttttt tctcctttta tctccgccag caaaacacct gccaactcgt cctctgcata
    15721 caaagcaaga atttgagttg ctcgattcaa ctccatatac aaaaaatatc ttccgtaaag
    15781 attcagcaga agttcatttt ccatatatct ctcaaagttc attccttcaa cagcaaaata
    15841 gatagctttt ttgtaatctt tcttttctaa gggtttaaaa gttagtttca tcatttcgcc
    15901 tcctcaactt caatgattgt gttatactta cattataata gacacagtgt ctactatcta
    15961 taatcaatca tctataagtt tgtcgattaa gcgacagctt tgctttgata tgtcggataa
    16021 ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa agctttggct
    16081 gaaacactat ccacattatt agtaaataaa aagattcaag caatcactat acgcgaacta
    16141 acagaaacgg cagatgtaca tcgttcgact ttttatacac attataagga tatctatgat
    16201 ctatatgat
//
LOCUS       DAZKCC010000061        16182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000061 DAZKCC010000000
VERSION     DAZKCC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.062"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..439
                     /gene="scm"
                     /locus_tag="ACG8VH_002061"
     CDS             <1..439
                     /gene="scm"
                     /locus_tag="ACG8VH_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9110608.1"
                     /translation="STTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSD
                     STDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDN
                     GNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(594..1580)
                     /locus_tag="ACG8VH_002062"
     CDS             complement(594..1580)
                     /locus_tag="ACG8VH_002062"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9110609.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1603..2169)
                     /locus_tag="ACG8VH_002063"
     CDS             complement(1603..2169)
                     /locus_tag="ACG8VH_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFP9110610.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2183..2599)
                     /locus_tag="ACG8VH_002064"
     CDS             complement(2183..2599)
                     /locus_tag="ACG8VH_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP9110611.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3056..4990)
                     /locus_tag="ACG8VH_002065"
     CDS             complement(3056..4990)
                     /locus_tag="ACG8VH_002065"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9110612.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5292..6227
                     /gene="pplA"
                     /locus_tag="ACG8VH_002066"
     CDS             5292..6227
                     /gene="pplA"
                     /locus_tag="ACG8VH_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFP9110613.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6317..7420
                     /locus_tag="ACG8VH_002067"
     CDS             6317..7420
                     /locus_tag="ACG8VH_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9110614.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7433..8386
                     /gene="menA"
                     /locus_tag="ACG8VH_002068"
     CDS             7433..8386
                     /gene="menA"
                     /locus_tag="ACG8VH_002068"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9110615.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9200..10546)
                     /gene="gor"
                     /locus_tag="ACG8VH_002069"
     CDS             complement(9200..10546)
                     /gene="gor"
                     /locus_tag="ACG8VH_002069"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9110616.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10680..11933)
                     /locus_tag="ACG8VH_002070"
     CDS             complement(10680..11933)
                     /locus_tag="ACG8VH_002070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9110617.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11939..12862)
                     /locus_tag="ACG8VH_002071"
     CDS             complement(11939..12862)
                     /locus_tag="ACG8VH_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9110618.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13083..15575)
                     /locus_tag="ACG8VH_002072"
     CDS             complement(13083..15575)
                     /locus_tag="ACG8VH_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP9110619.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15582..16049)
                     /locus_tag="ACG8VH_002073"
     CDS             complement(15582..16049)
                     /locus_tag="ACG8VH_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP9110620.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4753 a         3383 c         2877 g         5169 t
ORIGIN      
        1 atctaccaca acaagtgata gcacggacac gtcagcttca agtgacagta cagatactac
       61 ctctaccaca agtgatagct cagattcatc taccactaca agtgatagca cggacacgtc
      121 agcttcaagt gacagtacag atacatcctc taccacaagt gatagctcag attcatcaac
      181 ttcaagcgat agtacagaca cgtcgtctac cacaagcgat acacgtcatg caagtgtctc
      241 cttctcaaaa acaagttcgg ataacaggac gaactttaac ttaactaata aaacaagcaa
      301 tgacaatgga aacaaatcaa aaagagcact tcctaaaact ggaagtcaat cgaataattg
      361 gataacactc gcaggcgtta tattacttgt aatcggttta agaatcactt ttatgagtta
      421 taagaaatcg aaaaggtaaa aaaatattca agataaaaaa gagcaacgtc gctaacggac
      481 gttgctcttt atttttgctt tttatcataa atacaagaac tacaattcaa tttgtcaaaa
      541 aacacgtaga tttccttctg agaaatttat ttacgtatcg aacctttccc tattcagtct
      601 ttgcgatcca aaatcagttg ggtaagggaa aagagattct cttttgttcg aagagaggtg
      661 gcaggtaatt tctttatttc cttcaaagcc ttttctgtgt aagaggccgc taacttccta
      721 gcttcttcca cgcctcctgt atgttcaacg attttgcgta cggcgtcgac atcttcttct
      781 gtcattgccg ctttcttctc taaataaggt agtagctttg gtttgttttt ttgcaaagag
      841 tagatcaacg gtaaagaata gaccccttga cgcatgtctt ctagtacagg tttgccgatt
      901 tcctggctct cttttgtata atctaaaata tcatcgataa tctgaaaggc catcccgata
      961 gaaagtccga tatctcctgc tttttttgcg aagcgctcgc ttgttccact ttcaaaagcg
     1021 ccaacagaac aacttaattg gaacagttca gctgtcttac ctgaaatatt ttccaaatat
     1081 tcttttacag aaagttcata attatagcga tcatccatct gccctaattc gccattcaat
     1141 actttttcca tactgcgaga attcaattgg agacttttca aggaagtgga gtaatcagac
     1201 aatagtttaa aacagcagac aaataaatag tctcctgcat agacagaaac gacattacca
     1261 aactgactac ggatattagg ctgaccacgt cgcagatccg cttcatcaac gatatcatca
     1321 tggatcagtg tggccgtatg gagcatttcg atagaagctg ccaaagcgat ggctttgtct
     1381 cgatcttgct ctggtccaaa ttgtgagaac aatagctgat aggcaggacg caacagcttt
     1441 ccgccggcat ggatcgtttc tttgattgct tgttcgacag gtttgttttt caggcggaca
     1501 gtttcttcca tccgttttag tgtttctgtc aattctttac ttaaagcagg atagttttcc
     1561 cacattggat gtagcttcat aaaaaaactc cttgtgtgac gattattttt tggtaaacat
     1621 tgggctccat ttttgtttgg ttccctgctc ataatctttt tggaatcgtt gaagtgtctg
     1681 cacatgtcgt aaaagataat tcgctgcgat cccgattgca atccctgata aaatccctaa
     1741 aactgaaagg actggcaagt acaacatgac tgtccatgat tgtgcaatga aagaagccat
     1801 caaaagctgt ccgacattat gcatgaaacc gccggccgca ctaattccga tgatactgac
     1861 acgttttggt cctaattgct tgaccaataa cattccaaaa taactaagca aagcaccact
     1921 catactataa aggaatgtgg aaatcgttcc gcctagcaaa gtagtcagga atagtcgtag
     1981 ccaaagtagt aagaagctgt ctcgtttttt catggtaaaa atggcaacga tcgtgattag
     2041 attggctaag cctagttttg caccaggcgc aaaagaaaaa ggataaggga tcatattttc
     2101 caataaacca atgatcactc cttgtgcaac aagtagcgaa atataaatca ttttttgtaa
     2161 tttactcatg gtgtgtgaag gtctaataaa tcagactgtc ttcgtcacct ccatctgttg
     2221 aacggacttc gatcactaat ttatgtggca aacagacgat ggtttcgcca tttttcgttg
     2281 cccagcctcg acgtacgcag atctggtcat cgcaatctgc ttcttttatc cgtactcgat
     2341 cgccatctat ctcgatcaag ttataatctc cgtgttcatc tttgtaagta tatgtgttgc
     2401 tctcttctcc agccatcaaa gggaatgttt tgatttctgt gccatccaca cgcagtaccg
     2461 cttctttttc tgctgttgcg gtgttttgct gaagaccaaa aatcatgact ggtaaaaaag
     2521 aaagaacagt caaaagcaaa ataataaaaa catcccaagg tttgagcaga cttttttcag
     2581 caaattcttt aaggctcatt tttgttctcc tttttccaga ttaactcacc caattttagc
     2641 ataaaaacaa gaggttgtga cacctgtgtc ttgctttgag taataagaac gaagaataga
     2701 aaacagcttt ccatgttttc taattctttg ggcttattac cgagaagcag caggtggaac
     2761 accgtttata acaagaggtt gtgacagaag tgggcaactt caagaaataa gaaggaattt
     2821 acgaaaatta cttttcaagt ttttgtgaaa tttcggctta tttttcgaag aagctacttc
     2881 tggaacaccg tttataacaa gaggttgtaa cagaggttgt gacacctgtg tcttgctcat
     2941 ggaggcttat ctcgggcatc ttattccttt aattttacat aaaaaggagg ctgtgacggt
     3001 ttgtctcagc ccctttctat tgaaatcttg cattcaaata acgttcttta tctatttatt
     3061 tcttgccgta ctgggaactt ggtctaccgt accaccaatt acctagtgta cgttgtagcc
     3121 atggaattac ccagcgatcc aacccaatcg cacgtccgga accgttcatc aatgcgaaag
     3181 caacaaaaat gaaccaaatg tttacccagt aaaacatgcc tgataagcag aacgtgatca
     3241 ccaatacaat cgttgtcgca ctgcatagcc atgtgaataa tccagcaatc aatgctaatg
     3301 ccaaggcaat ttcaaagaat accatgaatt tttggaagaa cattgctaca tcattgtttg
     3361 gcatgatcac ttgcatgatc tttgtgaacc agtctggtga cttctcaatc acagccattg
     3421 gttcttcacc gtatgcataa ctcaaaccaa aatgtgcagc ttgagtcgct gcttctccgg
     3481 cacttgcccc tgctccgctg gcagctgtgg ttgcatcacc agcagcagaa gcaccactgg
     3541 caacatctgt tacattgtat tcccatgtcc aagggaacac actgtttcca gtgaaccatg
     3601 atccttcacc aaaccaagta cttggtttga acacttcacc attcccaatg atttttttgg
     3661 aagcttcaac gaaccaaact aaaccataga aaatacgaag tggcacactc cataagacat
     3721 tgccgtaacg agaagaatgt cctcgagtca cgttgcgatc atctttgata tggaaaaact
     3781 catgcattaa atattggaac atataataac cagaacgaat atcaaagaag tatttcaaat
     3841 tcacgatatg tttcattacg atagctaaaa atccgcttaa atggatctta tcaaataggt
     3901 tagctacccc ccatttggca ccaatagaaa ccataaatcc ttgatagttt cctttaaatt
     3961 tatgcttttc tccgcctttg atcgaagcca cgatattcgc tgcggctgtg tgtcctgttt
     4021 gttcagcagc ttgaacgatc tgcggtgtcg gtgttcctgg gaattcttca taaaagacta
     4081 aatcaccaat gatatagatg tttttatctt cgtagccttt tgcctgcata tattcattgg
     4141 cgatcaaccg ctgtccgcgt gctgattcta atccaaagtc agcagcgtca gaagttcctt
     4201 tgacaccagc tgtccaaatc agtgtgtgag tcggaatgtt gcgtccgtct ttcaatttga
     4261 tatgatcagc agccacttct acgattggtg cattcaataa taattccacg ttgtgcttac
     4321 gcaaatagcg ttcagctttt gcagcatcat ttcgatccaa catattcaag atcgtcggca
     4381 ttgcctctac gaccatcagt gtgatttcgt ttggatctaa tttataatct ttggctaggc
     4441 gatttttcca gtcgatcagt tccccaatca tttcaatacc ggtaaatcca gaaccacaga
     4501 caacgaaagt cagcatggct ttacgcagtt catcatctgg ttccaatgca gcttttgcta
     4561 ctgtcttctc aatatgttcc cgaatcctta cagaatcttc aaatgaccaa agggtgaatc
     4621 cgtgtgcttt gacaccaggt gtaccaaagt cattcggctc tccgcccatt ccaaggatca
     4681 gctgatcaaa aggataggaa cctaatttgg tcttgacgat ttttcgttct ttatcgatac
     4741 cgattacagt gtctgtgaca agttgtacat tcttttgacg agcaaacaaa tgctgcagat
     4801 cgtattggat tgcttctggt tctacacgtc cgccagctac ttcatgcaac tcggtcatca
     4861 tagtatggta cgaatgacga tcgatcagag taattgctac atcttctttt ttcagtttct
     4921 ttgccaagta ttttgtagct gaaacaccgg catatcctgc tcctacaacg acaatatttt
     4981 gctttgccat tgatcatccg ctccttatag actatctata taatgaatat atgataattc
     5041 ttttagtttg atacttatta cacaatctcc atatattata tagatgttct gttcttttgt
     5101 ctaactcctt ttggattttt ttcaaatttt aaaataaagc gttttttcac gaaatttgct
     5161 gaaagaaaac gtttgaaata ttacatgggt tatttatcaa aaatagttga cttataggct
     5221 aaaaagatta gaatgtataa tgaatcacaa agaaaaattg tggactagaa atggaatggt
     5281 ggggtattaa aatgaagaaa aaacgttttg taactggatt tgccgtatta gcattttcag
     5341 ctctagtgtt aggcgcttgc ggcgcagatg acaacggaag ctcaaactct tcttctgaat
     5401 catctacagc acaatcttcg actgcaaaaa gcacaacaga atcatctgca aaagttgtag
     5461 ctggcggaga tttgcaagac ggcacatata aattagaaga aaaaaattat tcaaatggtt
     5521 accgtgcagt ctttgaaatg gttgtaaaag acggcaaaat cactgaatca aaatatgata
     5581 acgtcaatga aaacggcgaa tcaaaaacaa aagatgcaga atacaacaaa aacatggaag
     5641 caaaatctgg cacaaaccca gaaaaataca ttcctgagtt gaatgaccaa ttcttaaaag
     5701 cacaaagcgc aagcggtgtg gaagtcgtaa ctggagctac tcactcatct gaaagcttcc
     5761 aaaactacgc acaacaattg atccaagctg ctcaagctgg taacacagat acaatcgaaa
     5821 tcgacaatgg tgctgatttg aaagacggca cttacaagct tgaagaaaag aactactcta
     5881 acggctaccg tgtgcaattc gaaatgacag ttgctggcgg caaagtaact gaatcaaact
     5941 ttgattatat cgacaaagac ggtaaatcaa aacaagatga tacagaatat aatgaaaata
     6001 tgaaagcaaa aagcggtaca gaaccaaaaa cttacatccc tactctgaac gacgaattcg
     6061 tcaaagcaat gggcgaagaa gatggctcac ctgctaatgt agaagttgtt acaggtgcta
     6121 cacacagctc acattcattc atcatgtacg cacaacaatt agtgaatgca gctgaaaaag
     6181 gcgacactca aacaatcgaa gtagataata tcgtaacaga aaaataatca cataatcata
     6241 ctaataaaag ttggagggtg ggacgatagc cagattaggc agtcgtctca cctttcaaaa
     6301 tgaagagagg aaaaagatgc ggaaaaaact actattttcc ttaatcacac tgggcatctc
     6361 ttttatggtg ctaactggct gcagcaactc agcaaataaa acaaccactg aaagttcagc
     6421 agctaccaag atattgaaag atccatattc tgatgaacag ttcttgttag ggacttacgt
     6481 tcgtatccgt gtgtatgatg aaggcaaaga atcagcattg aaacctgcct tcgatcgagt
     6541 aaaagaactt ggtgataaaa tcacgatcaa tcaaaaaggt tctgagatcg atgaagtgaa
     6601 tgagcaagct gggatcaaac cagtaaaagt ttctgatgac gtctacacgt tagtcaagcg
     6661 ggcctacgaa tatagccaag attctcaagg cggattcgat atggcgattg gtgcaatcac
     6721 acaattatgg cgaatcggtt ttgatgacgc cagaaaacct tcccaagaag aaatcgatca
     6781 ggcattgaaa ttagtggatt atcataagat cgaactaaat gacaaagaaa aaactgttta
     6841 tctaaaagaa aaaggaatga tcatcgattt aggtgcaatc gccaaaggtt acattacgga
     6901 cgaagtagtg aaagtcttga aaaaacaagg agtgaccact gccatcgttg acttgggcgg
     6961 taatgtctat gtcttaggcc acagcccaag aggcgaaaac caagactgga cagtcggcat
     7021 ccaagatccg aacatggctc gcggatctgt tttaggttct attaaagaac ggaacaaaac
     7081 attagttacc tctggaattt acgagcgtta tttagaagta gatggcaaaa aataccatca
     7141 tttgtttgat ccaaaaacgg gttatccgtt cgacaatgat atcgcaagtg tcacgatcat
     7201 aacagataaa tcaattgatg gtgacgggtt gtctacagca gtcttttcaa tgggtgtgaa
     7261 aaaaggactg gaatacgtcg aaagcgaatt gaataacggg acggaagcga tcttcgtgac
     7321 aaaagacgat aaagtatatg tgaccgatcc aatcaaagac accttcaaat tatcggagga
     7381 ctcccattac acgatgggtg accgttctga actaaaataa ggagtacgaa ccatgacact
     7441 aaaaatcttt ttagaagtag ttgaaatacg aacaaaggtg gctagtgtat ttccttttgc
     7501 ggtaggggta ttattctcta tcgctttttt tcatgagata cattggctaa acactttttt
     7561 attttttatt ggaatgctag tttttgattt agcgactacc gctatcaata attacatgga
     7621 ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg gccgcagaaa
     7681 tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg tttttgttat
     7741 cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat ttatctgttg
     7801 ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc ctttagggga
     7861 aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt atgtcaatac
     7921 attcggttca gatgtctatg atctgacagt caacttttca tctggacaat ttgctttggt
     7981 aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg tgtttacgat
     8041 tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg aaaatcatcg
     8101 ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc agctattgat
     8161 gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg aatggccgat
     8221 cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat tcaagcaaac
     8281 gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat tcaatgccag
     8341 ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc atgaagaggt
     8401 tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata gctccttata
     8461 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttatcagga
     8521 ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     8581 acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttatcag
     8641 aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa aatagttcct
     8701 catattttgc cgttttttga gctaataccg aaggacagct gattgaacac tatttgttcg
     8761 gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac ttgatttgat
     8821 tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt attcttttaa
     8881 catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta aacatctgtt
     8941 cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact taagagtgca
     9001 taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc tccaacttcc
     9061 ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta aaaaacaaaa
     9121 aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat aaaaagctgt
     9181 tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct gttggatgga
     9241 tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc acggcaaaac
     9301 cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt ctttcttctt
     9361 ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat gcaaagtaca
     9421 ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt tccaatgctt
     9481 cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact gtcggaacaa
     9541 gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat aaacgtctac
     9601 cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca acagcaaaaa
     9661 tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt ttgtccaatt
     9721 ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca ccggcaaaaa
     9781 gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat tcgtcattgt
     9841 ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct tcttggtaca
     9901 tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt tcttttcgat
     9961 atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca gccgcaatgt
    10021 atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa aatccatttg
    10081 aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct ccagtagcga
    10141 tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga ctatcgataa
    10201 aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt tgataagcac
    10261 cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa tcaacatttt
    10321 ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc aaaatcgtac
    10381 ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt ccgcccaatt
    10441 cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt gctgaagcaa
    10501 taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg ttcctcctta
    10561 ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa acaagtgtaa
    10621 cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca gctttttcct
    10681 tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg catagttaaa
    10741 atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg cataacttca
    10801 acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc tcctacagaa
    10861 aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga taatacttca
    10921 ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc gttttcagtt
    10981 ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca taactcgtcg
    11041 atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc tacagccgct
    11101 ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc ttcgatcttg
    11161 aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga catatccact
    11221 gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt tcgttcttga
    11281 ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca tccgccacgg
    11341 tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata agagatacac
    11401 atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat ctctgccact
    11461 tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc tttccagaat
    11521 tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg cagacctggg
    11581 gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc agaaataccg
    11641 acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc atgggtgacc
    11701 atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac tccttcagcc
    11761 atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt cccgccaata
    11821 tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt tcctgctggc
    11881 gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat catactcctt
    11941 atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg ttttcaggat
    12001 gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag ctaccttcta
    12061 aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt tcgcctggag
    12121 taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca ttcaattcat
    12181 tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct tcataaatag
    12241 aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca cgatcttttt
    12301 gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg gattgatgga
    12361 tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct tccatcgcct
    12421 tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct tttttcgccc
    12481 agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata tacggtatac
    12541 gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact gtgatcacat
    12601 ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt ttttcaggat
    12661 gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct aattctacca
    12721 attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat tgctcttcac
    12781 cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca atagtttcta
    12841 ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata gtagcatagc
    12901 tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat tatttcacaa
    12961 aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg tgacaaaaat
    13021 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
    13081 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
    13141 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
    13201 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
    13261 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
    13321 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
    13381 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
    13441 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
    13501 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
    13561 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
    13621 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
    13681 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
    13741 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
    13801 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
    13861 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
    13921 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
    13981 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
    14041 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
    14101 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
    14161 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
    14221 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
    14281 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
    14341 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
    14401 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
    14461 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
    14521 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
    14581 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
    14641 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
    14701 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
    14761 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
    14821 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
    14881 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
    14941 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
    15001 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
    15061 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
    15121 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
    15181 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
    15241 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
    15301 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
    15361 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
    15421 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
    15481 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
    15541 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
    15601 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
    15661 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
    15721 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
    15781 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
    15841 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
    15901 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
    15961 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
    16021 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
    16081 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
    16141 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAZKCC010000062        16153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000062 DAZKCC010000000
VERSION     DAZKCC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.063"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8VH_002074"
     CDS             226..894
                     /locus_tag="ACG8VH_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9110621.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8VH_002075"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8VH_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110622.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8VH_002076"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8VH_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9110623.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8VH_002077"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8VH_002077"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9110624.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8VH_002078"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8VH_002078"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9110625.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG8VH_002079"
     CDS             complement(5230..6684)
                     /locus_tag="ACG8VH_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP9110626.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG8VH_002080"
     CDS             6859..7764
                     /locus_tag="ACG8VH_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP9110627.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8VH_002081"
     CDS             7855..8532
                     /locus_tag="ACG8VH_002081"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9110628.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8VH_002082"
     CDS             8529..8699
                     /locus_tag="ACG8VH_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110629.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8VH_002083"
     CDS             8813..9709
                     /locus_tag="ACG8VH_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9110630.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8VH_002084"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8VH_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9110631.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8VH_002085"
     CDS             11372..12118
                     /locus_tag="ACG8VH_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9110632.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8VH_002086"
     CDS             12115..12954
                     /locus_tag="ACG8VH_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9110633.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8VH_002087"
     CDS             12955..13905
                     /locus_tag="ACG8VH_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9110634.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8VH_002088"
     CDS             14046..14798
                     /locus_tag="ACG8VH_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110635.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG8VH_002089"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG8VH_002089"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9110636.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5241 a         3011 c         3242 g         4659 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa cat
//
LOCUS       DAZKCC010000063        15803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000063 DAZKCC010000000
VERSION     DAZKCC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.064"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..1520
                     /locus_tag="ACG8VH_002090"
     CDS             93..1520
                     /locus_tag="ACG8VH_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110637.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1612..1977)
                     /locus_tag="ACG8VH_002091"
     CDS             complement(1612..1977)
                     /locus_tag="ACG8VH_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9110638.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1974..2339)
                     /locus_tag="ACG8VH_002092"
     CDS             complement(1974..2339)
                     /locus_tag="ACG8VH_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9110639.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2531..2779)
                     /locus_tag="ACG8VH_002093"
     CDS             complement(2531..2779)
                     /locus_tag="ACG8VH_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110640.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2778..2906
                     /locus_tag="ACG8VH_002094"
     CDS             2778..2906
                     /locus_tag="ACG8VH_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP9110641.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2963..4657
                     /locus_tag="ACG8VH_002095"
     CDS             2963..4657
                     /locus_tag="ACG8VH_002095"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP9110642.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4724..4930
                     /locus_tag="ACG8VH_002096"
     CDS             4724..4930
                     /locus_tag="ACG8VH_002096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110643.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5308..6441
                     /locus_tag="ACG8VH_002097"
     CDS             5308..6441
                     /locus_tag="ACG8VH_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9110644.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6472..6792
                     /locus_tag="ACG8VH_002098"
     CDS             6472..6792
                     /locus_tag="ACG8VH_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP9110645.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6821..7285)
                     /locus_tag="ACG8VH_002099"
     CDS             complement(6821..7285)
                     /locus_tag="ACG8VH_002099"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9110646.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7417..8670)
                     /locus_tag="ACG8VH_002100"
     CDS             complement(7417..8670)
                     /locus_tag="ACG8VH_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9110647.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8699..9016)
                     /locus_tag="ACG8VH_002101"
     CDS             complement(8699..9016)
                     /locus_tag="ACG8VH_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9110648.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9042..9362)
                     /locus_tag="ACG8VH_002102"
     CDS             complement(9042..9362)
                     /locus_tag="ACG8VH_002102"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9110649.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9826..11241)
                     /locus_tag="ACG8VH_002103"
     CDS             complement(9826..11241)
                     /locus_tag="ACG8VH_002103"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9110650.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11258..12574)
                     /locus_tag="ACG8VH_002104"
     CDS             complement(11258..12574)
                     /locus_tag="ACG8VH_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9110651.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12724..13422)
                     /locus_tag="ACG8VH_002105"
     CDS             complement(12724..13422)
                     /locus_tag="ACG8VH_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9110652.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13711..14700)
                     /locus_tag="ACG8VH_002106"
     CDS             complement(13711..14700)
                     /locus_tag="ACG8VH_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9110653.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14780..15613)
                     /gene="msrA"
                     /locus_tag="ACG8VH_002107"
     CDS             complement(14780..15613)
                     /gene="msrA"
                     /locus_tag="ACG8VH_002107"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9110654.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4920 a         3197 c         2700 g         4986 t
ORIGIN      
        1 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
       61 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
      121 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
      181 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
      241 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
      301 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
      361 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
      421 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt
      481 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
      541 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
      601 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
      661 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
      721 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
      781 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
      841 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
      901 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
      961 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
     1021 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
     1081 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
     1141 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
     1201 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
     1261 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
     1321 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
     1381 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
     1441 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
     1501 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
     1561 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
     1621 ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
     1681 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
     1741 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
     1801 agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
     1861 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
     1921 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
     1981 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
     2041 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
     2101 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
     2161 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
     2221 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
     2281 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
     2341 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
     2401 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
     2461 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
     2521 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
     2581 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
     2641 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
     2701 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
     2761 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
     2821 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
     2881 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
     2941 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
     3001 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
     3061 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
     3121 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
     3181 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
     3241 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
     3301 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
     3361 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
     3421 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
     3481 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
     3541 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
     3601 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
     3661 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
     3721 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
     3781 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
     3841 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
     3901 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
     3961 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
     4021 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
     4081 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
     4141 tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
     4201 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
     4261 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
     4321 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
     4381 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
     4441 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
     4501 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
     4561 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
     4621 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
     4681 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
     4741 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc
     4801 tgccctgcat caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg
     4861 gttagactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa
     4921 aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca
     4981 taaatttgac ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt
     5041 ctgtttcttt ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca
     5101 tctaacggat acaactattt caatgattac cattacacta aaaaatcgga aacacacctc
     5161 tccgaacaca aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta
     5221 tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat
     5281 tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa
     5341 atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt
     5401 atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt
     5461 ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct
     5521 caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt
     5581 cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag
     5641 ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg
     5701 gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt
     5761 cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa
     5821 aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta
     5881 ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta
     5941 cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac
     6001 acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc
     6061 gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc
     6121 ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga
     6181 acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt
     6241 tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa
     6301 atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg
     6361 ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata
     6421 aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa
     6481 atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga
     6541 cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta
     6601 ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga
     6661 tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga
     6721 atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa
     6781 aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt
     6841 gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt
     6901 atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat
     6961 gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg
     7021 gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt
     7081 caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag
     7141 gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa
     7201 ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta
     7261 atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa
     7321 aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa
     7381 cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct
     7441 tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa
     7501 attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat
     7561 tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc
     7621 aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg
     7681 gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc
     7741 aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc
     7801 taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg
     7861 tttgttcacg ataaacggtg cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat
     7921 ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa
     7981 gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag
     8041 tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa
     8101 tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa
     8161 tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt
     8221 ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat
     8281 aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga
     8341 aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa
     8401 gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc
     8461 tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa
     8521 gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga
     8581 gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg
     8641 tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt
     8701 acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag
     8761 atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt
     8821 gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg
     8881 catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt
     8941 ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat
     9001 ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt
     9061 tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca
     9121 tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact
     9181 tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa
     9241 gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt
     9301 gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc
     9361 attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt
     9421 taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac
     9481 actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta
     9541 acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat
     9601 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt
     9661 tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg
     9721 tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt
     9781 tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc
     9841 gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt
     9901 caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa
     9961 tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc
    10021 gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta
    10081 atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc
    10141 gttttctgtt atcagccaag ggatattatg ataattttct tttagataca tggctatatc
    10201 atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa
    10261 atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg
    10321 aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg
    10381 ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa
    10441 ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata
    10501 aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac
    10561 catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc
    10621 tactgcttta gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt
    10681 ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc
    10741 gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata
    10801 cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa
    10861 agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga
    10921 gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc
    10981 agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat
    11041 cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc
    11101 attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc
    11161 tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt
    11221 ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac
    11281 gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt
    11341 gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca
    11401 ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac
    11461 caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca
    11521 tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata
    11581 acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac
    11641 cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa
    11701 tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa
    11761 gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga
    11821 cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg
    11881 ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga
    11941 taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac
    12001 tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc
    12061 tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt
    12121 tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga ttttcattca
    12181 ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga
    12241 atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga
    12301 tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta
    12361 aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa
    12421 acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta
    12481 tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta
    12541 atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc
    12601 tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt
    12661 tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa
    12721 ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat
    12781 atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat
    12841 ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa
    12901 caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc
    12961 ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg
    13021 aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc
    13081 atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc
    13141 tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc
    13201 tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc
    13261 tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc
    13321 atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat
    13381 cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc
    13441 atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta
    13501 tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa
    13561 gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat
    13621 gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat
    13681 atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc
    13741 ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa
    13801 ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc
    13861 catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc
    13921 cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga
    13981 atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa
    14041 cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc
    14101 acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa
    14161 aatttcagtc attttttttg tatcttctcc attatagttg gatataccaa tatataatgc
    14221 ttttccttgt cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg
    14281 gtctgggcga tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg
    14341 gtcacaactt gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat
    14401 atgataacct gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct
    14461 aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag
    14521 gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat
    14581 aggatcataa tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc
    14641 tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa
    14701 tttttctcct cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact
    14761 acattttaaa gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt
    14821 tttttactcg ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt
    14881 tcggttgttt tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg
    14941 gttcgatttt tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg
    15001 attgttccgc taattctttt tgtcgttcga cttgataaaa aatcactgga cgatattgct
    15061 tccctctatc ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg
    15121 tcagcaactc gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat
    15181 gacctgtgta accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt
    15241 atcctgacaa aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga
    15301 aacatccccc tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg
    15361 aaaagtcata tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta
    15421 catcaggcgt cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata
    15481 actgttcttt aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc
    15541 gttcccaatc tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt
    15601 gttcctcatc catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta
    15661 atttatcata aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa
    15721 aaaagagaga atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa
    15781 agttaattta ccttttatgg aaa
//
LOCUS       DAZKCC010000064        15742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000064 DAZKCC010000000
VERSION     DAZKCC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.065"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8VH_002108"
     CDS             complement(24..722)
                     /locus_tag="ACG8VH_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP9110655.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8VH_002109"
     CDS             complement(715..2067)
                     /locus_tag="ACG8VH_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP9110656.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8VH_002110"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8VH_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9110657.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8VH_002111"
     CDS             3851..4588
                     /locus_tag="ACG8VH_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP9110658.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8VH_002112"
     CDS             4713..5192
                     /locus_tag="ACG8VH_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP9110659.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8VH_002113"
     CDS             5189..5956
                     /locus_tag="ACG8VH_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP9110660.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8VH_002114"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8VH_002114"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9110661.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8VH_002115"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8VH_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9110662.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8VH_002116"
     CDS             7567..7797
                     /locus_tag="ACG8VH_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110663.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8VH_002117"
     CDS             7788..8036
                     /locus_tag="ACG8VH_002117"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9110664.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8VH_002118"
     CDS             8142..8726
                     /locus_tag="ACG8VH_002118"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9110665.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8VH_002119"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8VH_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9110666.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8VH_002120"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8VH_002120"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9110667.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8VH_002121"
     CDS             10627..10806
                     /locus_tag="ACG8VH_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110668.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8VH_002122"
     CDS             10828..11853
                     /locus_tag="ACG8VH_002122"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9110669.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8VH_002123"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8VH_002123"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9110670.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8VH_002124"
     CDS             13425..14372
                     /locus_tag="ACG8VH_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP9110671.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8VH_002125"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8VH_002125"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9110672.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8VH_002126"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8VH_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110673.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8VH_002127"
     tRNA            15637..15709
                     /locus_tag="ACG8VH_002127"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DAZKCC010000065        15509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000065 DAZKCC010000000
VERSION     DAZKCC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.066"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1017..1358)
                     /locus_tag="ACG8VH_002128"
     CDS             complement(1017..1358)
                     /locus_tag="ACG8VH_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9110674.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1358..1588)
                     /locus_tag="ACG8VH_002129"
     CDS             complement(1358..1588)
                     /locus_tag="ACG8VH_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9110675.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1875..2213
                     /locus_tag="ACG8VH_002130"
     CDS             1875..2213
                     /locus_tag="ACG8VH_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110676.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2418..2980)
                     /locus_tag="ACG8VH_002131"
                     /pseudo
     CDS             complement(2418..2980)
                     /locus_tag="ACG8VH_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3712..4293)
                     /locus_tag="ACG8VH_002132"
     CDS             complement(3712..4293)
                     /locus_tag="ACG8VH_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9110677.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4476..5102)
                     /locus_tag="ACG8VH_002133"
     CDS             complement(4476..5102)
                     /locus_tag="ACG8VH_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9110678.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5124..6455)
                     /locus_tag="ACG8VH_002134"
     CDS             complement(5124..6455)
                     /locus_tag="ACG8VH_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP9110679.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6610..7011)
                     /locus_tag="ACG8VH_002135"
     CDS             complement(6610..7011)
                     /locus_tag="ACG8VH_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9110680.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7097..7303)
                     /locus_tag="ACG8VH_002136"
     CDS             complement(7097..7303)
                     /locus_tag="ACG8VH_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110681.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7398..8900)
                     /locus_tag="ACG8VH_002137"
     CDS             complement(7398..8900)
                     /locus_tag="ACG8VH_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP9110682.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8918..9373)
                     /gene="argR"
                     /locus_tag="ACG8VH_002138"
     CDS             complement(8918..9373)
                     /gene="argR"
                     /locus_tag="ACG8VH_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9110683.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9471..10088)
                     /locus_tag="ACG8VH_002139"
     CDS             complement(9471..10088)
                     /locus_tag="ACG8VH_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9110684.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10309..11682
                     /locus_tag="ACG8VH_002140"
     CDS             10309..11682
                     /locus_tag="ACG8VH_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9110685.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11757..12869
                     /locus_tag="ACG8VH_002141"
     CDS             11757..12869
                     /locus_tag="ACG8VH_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9110686.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12941..14578)
                     /locus_tag="ACG8VH_002142"
     CDS             complement(12941..14578)
                     /locus_tag="ACG8VH_002142"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9110687.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14746..15354)
                     /locus_tag="ACG8VH_002143"
     CDS             complement(14746..15354)
                     /locus_tag="ACG8VH_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9110688.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4307 a         3173 c         2697 g         5332 t
ORIGIN      
        1 taaccaacag caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc attaataaat
       61 gttgaaaaat tcagcaactc attactgtat gattttagca gcccaaccaa catataccct
      121 ataaaagcta ataagatggt taggaatcct ttagaatttt tactcattca tagacacttc
      181 cttaaataac ttttattata gaaggtattg taaataataa tattgacaaa aagtgactca
      241 aaataaaaaa aaccttacat tatactggaa tgaactgtat tgatagtgta cctaaaagct
      301 ctactgacat aagatcgtgt aatcggtaaa gtaattttat taattatacg ggatgtaatc
      361 aattagagaa taaactaatc ataaaaggta cttttacatt cataattgtc gctttgagat
      421 tctactttgg ttcactatct gacccaattg cacattttcg atcattacaa gtattggtgt
      481 cgttagaaca actaaataaa aactagcggt agatttttca gaatctatgc taatttctgt
      541 atggatgttc cagtgatggc aaatttctct tttcattgtt tttcttgtcg catgatatgc
      601 ctttttgtgt tattcagtta aactatagaa attctttcct agacagatct tgatcccaaa
      661 ttttacgttt ttgagcatcg atatagttta aaatgacatt actcatatct aaagagttta
      721 gattttttag gactaaaact gtcagttgtt ctttttcttc tttctgttaa agtattgtcg
      781 cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa gttttgtgac acaaaaaaac
      841 gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat gctatatatt ctttagttta
      901 agatacggaa attaatgcca tcgaccacac tagtagaatg aacgacattt acttggcctt
      961 tttttaattt cgttatgttt agcatataaa aatattatag agtttactta caatgcttaa
     1021 aacatatact gtataatctg atcaatttta gccatgtctt gcaatggtaa gttttctatt
     1081 tttttgagtt ttcgttcctt aaaatcaaga gattttaatt gtgtaataat tacttgtcct
     1141 tttatatcta gctcttcagg taatgagtaa cgtgtcggta aattctttat agtggaagta
     1201 attggacata tgaccgaaaa cattgtttta cggttaaatt cataacgaga gacgactagt
     1261 cctggtcgtc ttttttgaat ttcttttcct acggagggat caaaatcaat ccatacaata
     1321 tcaccttttt taggaatata aattctttct tctgacacta taaaatttct ctcccttcag
     1381 gagttaaatc ttcccattcg tctttctcat aatactcggc ctcttcccca ccactaaatg
     1441 gatcttcaat ttttggaact aacgtaattg ttccatcatc agaatacaca acgatatatt
     1501 cttggttttc tgaaggtttc tttccgttat cagaaggtaa agttaccact acagaacttc
     1561 cttgtaatct agattttgta acaagcatta tcatcactcc cttaataaaa gtatactata
     1621 taacactaag taacgcaaaa ttcccctttg taaactatct agaaaattgt tttgtttaaa
     1681 gtatatctta ttggatataa atagaaataa attgtagcca gttcattttt tctttaacta
     1741 aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa tactatagct
     1801 aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt tcaaagacaa
     1861 caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt tgctctgaca
     1921 aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatca
     1981 aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga caagactttg
     2041 ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa
     2101 ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa tatacaactg
     2161 ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca taatgataaa
     2221 catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat agaagctata
     2281 cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg agcatttctt
     2341 ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct
     2401 ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg ctgagtaatg
     2461 atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc agtcaagaca
     2521 agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt
     2581 gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt
     2641 tccttatttg aaaacataaa tttgtctttt tcttcattcc actcattggg aaaacacaac
     2701 gattttgcaa aaatggttga tgaaaggtct ttttttcttc tactgtctct gcacgggcat
     2761 taatcatcaa ttgtcctttt ttaaatccgg taaatcccca cttcatgatt tccggagtga
     2821 ggatgttttc ttcctctttt cttaacgtca caacaagatt tgacggaaaa acttctccag
     2881 ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa taattctgaa
     2941 ggagctctga ctctagatca aaaaaataac gaccacacat cctaatccct cccaatcttt
     3001 ttttctatca tacactgcaa caagaagttc gcaccaacaa aacgctttca atttttgtag
     3061 aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa tggtattctc ctgccaactc
     3121 ctcggtcaca agtcacaata ttcaacaaac atgagaagct gctttttgaa attgttcctt
     3181 gtgactcgga gtgaaacggc aggcttacga cctctgggta aacggtgttc aaaagccagt
     3241 ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ctattttcac aaaatttttc
     3301 ttaatactcg aaactaagcg actttttcac aacctcttcc ttaaacggtg ttcaaaagct
     3361 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gcagctattt ttatttggct
     3421 gttcttatta cttgatgcag ggcagctttt tcacaacctc tccactaaac ggtgttcaat
     3481 cagctaacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt
     3541 gtttctctaa tactcaggtc aaagcgctga tttcacaacc tctaatacac ggtgttccag
     3601 aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
     3661 aattccttct tatttcttga agctgaccac ttctgtcaca acctctaatt attaaagaac
     3721 cttttgcgaa gttaacaatc tttctttgat caattgaaaa ttctgcctaa gtagtgatag
     3781 atcctgtgtg actttcaaca ataagatact tccctggatt tcagcaatca acgctttggc
     3841 ttgacggtct gcttcttcat cggacagttt ttcttcagaa agtgccagga tattttgttt
     3901 cgtatacttg acccattgat tgactaattc tagtagcaac agacggtatt cttcatccac
     3961 tgtactcaac tcaataatca aattacctac aggacagcca tgataagtga tctgtgtctc
     4021 atgatcagcg aaaatccagt ccaacatttc ttctaatgct tcttctggct gttttccagc
     4081 atttaagatg tcttcgaaca attctgtctg ccatacgtcc atatgcgcat ggatcaccgc
     4141 tagacaaact tctttttttg aagaaaagta ataataaaat tgaccttttc ctgtatcaga
     4201 agctgctaga atatctcgga ttgacgtttc atgatagcct tttttataaa taagatcttt
     4261 ggtcgcttcc aagatctttt cctttgtttt caatgatatt ctcctttttc atttctcata
     4321 gtcctatatt atacagtaaa ttcatcattt tttcttctta atcgtttaaa aaagactgtg
     4381 ccaaattatc tgacacagcc ctcactataa aactgttctc atatagctag atgttcattg
     4441 attcaagaac tgcgaggaca aaagcaattc ctatcttatg atagttgctc taatacttcc
     4501 tctgttgtca tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc atccattgct
     4561 tcttggccag gagcaccaat tgcatctgtc gctaccacaa cattgaatcc actctctacc
     4621 aattcacgca tatgggaatc gctacacaag tttccgttca ttccaccaag aattactgtg
     4681 tcgattccat tttttctcaa ttggagcgcc aaatcattgc tttctggtcc aaagatttta
     4741 tgaggagagc tgatgatcgt attttcatcc aaataaggtt tcaattcttc gataaaatcg
     4801 gctcctgaac cttcttccac tgattcatat tgactttttc tagcaaacat ctgctgctca
     4861 agcatcatat gttctcctgc accgccaaat tgccatttat ggtcatgtgg gtaaaaataa
     4921 tgaggagaaa taaagagttt gtatcctttt tcttttgctt gttttaccat ttcaatcata
     4981 ttttcgatcg tattgtattt ttctaagata tcttttgtca aaccaaagcc accgcctgct
     5041 ggtttcaaga actcgacttg cgggtcagtg agaactactg ctgtattttt attattgaac
     5101 atggtggatt ctccattcgt tatttatttt aatacatcat ttagagcttc actcattgtc
     5161 ggatgagaat agatttgatc tctcaataac gtataaggaa gttttccatt catagctagc
     5221 gaaatcaagt tgatgacttc atgagaatct tccgcataga tacttgcccc tagaatcaag
     5281 tcagtttcag ggtcgactaa aactttgaat acacctttag gatcttctaa tacttgtgct
     5341 ttagggacac cgcttgtcat atacttgaat aatttgtaat tgacgccttg tgctttggct
     5401 tgtttttctg ttaaacctac attggataaa gtcggagtga taaatacact gtaaggcaca
     5461 gctttccggt tattcgttgt tcgtttattc tcgcccttca actgatccat aatgattcga
     5521 taatcatcta gagagatata agtgaactgc aatccgcctt tgacgtctcc aatcgcccaa
     5581 atgttgtctg catctgtacg aaggtattca tccacagcga ttgctccgcg atctgttagt
     5641 ttaacatctg tattttctaa tccaagtgat gctgtacttg gttttcttcc agttgcagcc
     5701 aaaacttttg acgcttggat tgttacttct tggccatctt ctgtaaaggt gacggcagct
     5761 gttgattctt ggtctgctac ttgatctaca gatacaccta gatggaattc gattcccgca
     5821 tttgtcatgt cttctaaaat catttgcgcg atatcttcat cttcacgtgg aagaaattca
     5881 gaatgggcat caagaacgac aactttagat ccgtattcat tgaacattga tgcaaattcc
     5941 aagccaatat atccagcccc gatgatcact aatttttccg gtaattgttc ctgatccatc
     6001 gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc ctttgattgg taaaatgatt
     6061 ggttcagaac cagtattgat gaaaatccgt tcgccttcaa caatcacttt ttcaccatta
     6121 ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg taccgtccat cactgtccca
     6181 gttgcctcat cgctgatcat atgataattt ttggcgcgaa gcatgcccgt caatttttct
     6241 ttccgagcaa cagcctctgt aaagtcgatt cctctttctc cattgaaaat caatgattta
     6301 gaaggaatac agccgatgtt gatacatgtt ccgccataca tacgtgttga cttttcaacg
     6361 actaaaaccg attcaccttt tgaagctaac gccttcgcta aagtcttacc gccctttcca
     6421 aaaccgatca caatattttg atatttttcc atcatttttc cctcgcttct tttttaccta
     6481 ccgattggta caaaaatatg ataaccgata attgtctatt ctccaaatca tctgctttat
     6541 ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt tttagtctgg ttcgatttcc
     6601 tttattcttc taaacaatcg ttacttctac atcgatattt cctctagttg ctttggagta
     6661 agggcagaat tcatgcgcat cttctgcaat tttttgggct tcttcttcgc tgatgccatc
     6721 gatatgcact tctaatacta caccgatctg atagccgcca tcatctaaat tataaagaga
     6781 gacacgtgct tttactgtgg actctgctgt cattcctgct tctttgatca tatgttgtac
     6841 cgccccattg aaacaagaag cataacctgc tgcaaataat tgttctggat ttgttccttc
     6901 tgcatggatt cctggaggag tgattttcat atgcatcgag ccatttggtg cctcgacttc
     6961 tccattgcgc ccgccatggt tgatcattgc tgtttcgtat gcttttttca ttgtactgct
     7021 cctttcgatt gaccatttgc tacttggcta taattatagt gtatgacaag taaggcgctt
     7081 tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc ttctactatt tttccaaaaa
     7141 acaaaaaaat actactcgtg aacaacagcg tcatatttgt tccaaaataa atagcaatct
     7201 tttctacata atcttttata tgattgattt taagatacat atataccaga aagttgaccc
     7261 cataaaccaa tactgttaaa aaaataacca tattcgttac cattttttcc tccttttgct
     7321 ggtctcccta ccagcactcg atttaaccat accacttttt ttgccgtcca agcaactagc
     7381 ctttcaaaaa gactggttta ttctgctgtt aatgattcaa gatcagaaaa atgtccgaat
     7441 aattttgcta attttactaa cgccaacttg ttctttccga attcatcatt ttctatctgt
     7501 aatactagta acggctcatg taagcttaaa cggatcaaaa accagccgtt tccgtatggc
     7561 tcttctagat catatcgaat accttcttca ttttccgggt cgaagttcat ccctttttct
     7621 tcttccaaca gtcgcttcat ttcttctaat atcttgtttc cttgttcgcg tggttcatcg
     7681 ctgaacaatt ttaatcggat ttcttgtact tctacgggct gtttcaactc tttgatttgt
     7741 tcatctaatg tgataccttt tctttttaat tctggcaaaa gcatcaggat ctttgcgatg
     7801 acatatgcac catcatccag aaaatagttt tccttgaatg cagcgtgtcc gcttgtttcg
     7861 atggctagct gtgtatcttt cccaagacgg ttaagttcta atgctttatt gattacattt
     7921 cgatagccgg aaatataacg aacttggatg cctccttttt cttcaatgaa tctttgtaga
     7981 tgagaagaag taggggaatt cgtcacgatt cttgttcctg gatgctcttt taatacgatt
     8041 gtacttaata ctgcgatcaa attatttcga ttgatcactg ttccagaagc actgacaact
     8101 gcagaacgat ccacatctgt atcgaaaatg atcccaagat ctgcttcatt tgctaaaaca
     8161 gcctgcttga tactttccat cgcttcttta ttatcaggat ttgggatatg attaggaaat
     8221 cggccatctg gttccaaaaa ttgggaacct gttgaatccg cacctaagct attcaatact
     8281 ttttcagcaa agaatccacc agcaccattt ccggcatcaa caatgatttt ccaaccattt
     8341 agtggtttgc tttctagtgt tccaatcccc tttttaattt ttgtgactaa atcttgcgca
     8401 taaggtgtca aaaggtctgc tttttctacg cttccagttt gttcgctttt tattttttgc
     8461 tgctgtgata ggataaaagc aatatcttct ttttctgctc ctccttctga ggtaaagaat
     8521 tttattccat tataataata gggaagatgg ctagcagtca gcataaccgc agcatcgcac
     8581 gagaactgtt caaattgagt ggacataaac atagcaggcg tagtagctaa ttcgaaatcg
     8641 atgacatgta tcccttgttc tttcagactt tggatcaacg cctctttcag tgctggtccg
     8701 cttatcctac tgtcatgacc aatcccaatc gtccattttt tatttggatc ggttgatttc
     8761 ttctttttct ttatccagtt tgctagtccc cagccgatct tttttaccat gtctgtcgtc
     8821 aagtttactt catatccttc aactggtaat gcgattcctc gaatatccga tccattttgc
     8881 agcgctttca tatctttcat ataaaagtcc tcctcattta ttccctcatt ttttccagct
     8941 tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat aataatgaat gtatctacac
     9001 cggccatcgt tccgacaact tcagggtaag ccacttcatc tagccaattc gttaccacat
     9061 cggcaccatt tcgttcagtc aatacgataa ccatgaactg aacagactgg atacgcaaaa
     9121 cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa agaaacagat ggctggttaa
     9181 ataatgtata gcgtactatt ttgttttcat tttgactttt tactaacttc atttctcgaa
     9241 tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc ttcatgcagc cgcgcaagca
     9301 gctcttcctg tgtcccaata ggttgttctg taatgatctg gcggatcagt gcttgtcgct
     9361 gtttcttttt catattagac acctctcaat gttgattata gcaaaagata agtaatattt
     9421 cttcatattt tggtattttt tacttttatt ttttgttttc attcaggttg ctaacctttt
     9481 tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg ctgattttct tggatcgctg
     9541 atgtaaatct ctttgtgttc tttagaagct ctttcatagc cttcttttgc gatgaatgct
     9601 tctaatttct caaacgttgc tggttcttcg gcatagggcc cgacgtgtaa gatttgggca
     9661 caaagacctt cttccatctt tttgaatact acttgttctt tcaagtcttc cggaagtttg
     9721 ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa caaagtcagg ctgtcggatc
     9781 atcaattgat aagaaaaatg ttcttttttc atcactggtt catgcaaata cttttcttgc
     9841 agtccccatt gtccttccag tggatagaca gtatacatct gataatcagg aatcagccaa
     9901 ttttttttag gcgacatgcg tattgcatag ctgactgcat atagacaggc tactcttcgt
     9961 ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc taaagaatga ttttttagga
    10021 acctctagta tttgcgggct ttttttcata ctatagctct tgtctttacg ccattcaatt
    10081 ttttccattt ttcttcctcc gcgaaattcc atttagttat ttattatcat acctcttttt
    10141 gagtgacttg gctaatcgtg ttgacactat ttttcagttt gtgctagaat aagtgaagta
    10201 aataaatcgt acagataaca cgtttcggcg tgtcgagaga gaggcggttt ctatttttat
    10261 aatagaaatc actctctttt tttacgaaat tttttagagg tgatgtaaat gacaaaacaa
    10321 aaacagctgc ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt tgcgggcgta
    10381 ttgatcgaga cggccatgaa tgtaactttc ccaactctta tcagacagtt tgatttaacg
    10441 actgcacaag tccaatgggt aacaacgatt tatcttttga tgatttcgat cattgttccg
    10501 ctgtctactt atttgaatcg caattttagt ctcaaaaaac tctttttagc ttcaaatctg
    10561 cttttcttgg caggtataat gatcgatttc ttctctccta ccttcatggt cctattgttt
    10621 ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt ccatattatc
    10681 ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac tttgacaacg
    10741 gccattgctc cagcaattgg ccccacttat ggaggcatta tgacttcaca ttttacatgg
    10801 aatcatatct tcctgtttct tgtgcccgtt ttgtttcttt ctttgattat cggctggttt
    10861 gctattcccg agatggcagt acaaaaaagt ggttatttgg attggataag cgttttagga
    10921 atttgtcttt tgtttagtgg aagtctaaca tttttgagta cgttgggaac agtttccagc
    10981 tggattgctt taatcatcgg cggtattggc tggttccttt tttactatcg aagtaaaaaa
    11041 gtggagcatc cactgatccg actgtctatt ctttcaaata aaacattccg cttgtttttg
    11101 attggcttct tagtttatca gtttttatta cttggtgtat cttttgtctt gccgaatttc
    11161 cttcagatcg ttcaaggaag tagtcctttt gtcgctggat tagcgatgct tcccggcgca
    11221 gcggttggtg ctttgctttc tcctttttcc gggaaaatgc tggatcaata tggtccgaag
    11281 aagccaattt ttgctgggct gattctttct ttgattggtt ggctagcatt attcctactc
    11341 ataggacacg cttcagtggg tgtattgatt gcttgtcatg tgttttatat gatcggtatc
    11401 ggattatctt acagcaatat gatgaccacc ggaatgaatg cactgaatga agaactacaa
    11461 ggagacggga acgcaatgtt caataccttg cagcagtttt ctggcgctgt ggctacttca
    11521 ttagtagcgg tcattatcaa ttacgtccag catcatactt cccataacta tgaagtgtct
    11581 actacattag gatcaaaagc agcattaggc gtcttgttgc tgttgcttct cgtaagcttt
    11641 gctcgattcg cctattatct gttctttgct aaaaaagcgt agattccata tacggcaggt
    11701 gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc ttttttatgg
    11761 aaaaaattgc cattatcggt ggcgggatca ttggtatgac cctagccagt tacttagata
    11821 catcaaaatt caatgttgct ttatttgatg aagggacagg acaggcaact aaagctagcg
    11881 ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat cgtctggcta
    11941 aagacggcgc tgcttttttc cctaaactta taaaagattt ccaacttgat caaacgattt
    12001 acgcacaatc agggacactt ttgcttcgac ctgaaaaaga tttagctgaa ttggctgttc
    12061 ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa catcgaatgg ctttcacctg
    12121 aacagacatc tcaagcactt ccgttattga aaccagcagt ttcactgcgt atcagtggtg
    12181 gtggtcgtct ggatggaaaa gcatatttag aacatcttgg tcagcgttta gcacaaaaac
    12241 aaatggagca gatccaggca aaagtcactt tgcataagaa gagcgattct tggctcatca
    12301 agggaccgtt tggagaatac acagctgatc gtgtaatttt gacacctggc ccggcactta
    12361 agcctttatt acaagaatta ggctatgctg cagatatacg tccgcaaaaa gggcagctct
    12421 tagcatttca gacatcttat aagatgagtc aagactggcc tgttgctatg cttgacggag
    12481 aagctgattt cattcctttc atggatggaa agatcctttt aggtgctacc catgaaaacg
    12541 atcaaggctg ggatttggct cctactagcg aagcttataa gggattgact aagagtacag
    12601 aacagttctt gactgatcca gatcagataa aaaactggcc atacgagttc cgtgtaggta
    12661 cccgtgctta tacgtcagat ttttccccat ttttcggcgt actgccagat gatccaagct
    12721 tactggctgc tagcggtcta ggctcttctg gcttgaccac aggtccttat atcggctatc
    12781 tattagccaa cttcttaaat actggtcatt ggcctacgga agattatcaa aaggacatct
    12841 ccacctatat tacgaaaaat ccatcataga aaaacagctt tttcaagtaa ggatcccgag
    12901 cgatattgcg catggattac ttcttgaaaa agctgttatt ttagttcaat ttctgaatca
    12961 aattctccaa actttctttt gcttcatctg gcaagagatc ttttcctatc tgagcttcaa
    13021 tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt tttataggac gtatcctcaa
    13081 aagaaacagg aaaatctggt ataggtaaat agtggatgtc ttcaaatgga ccaaacgact
    13141 tgaattctgc cgtttgttgc acgattcttt caacgattga caatgtcgtt tcaggtttga
    13201 tttttttatc atcgaaatat ccagtattca ggacatagca ggctgtttgc tgtttcaaaa
    13261 acaactctcg aaaagcttga tagtcttccc taagaggata gacacggaaa ggattagcaa
    13321 atggttcaat gactagctca tctagctgtc tttttgtagt gatattttcc gcgtctgatc
    13381 gttttgttgc taatgttgca ccaaaagacg ctgctaaaac tgggtcattg atcttgataa
    13441 ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt tcgctaagat
    13501 gatcgactcg gtcgggtgtc gcataacgtg atttgactgt ccgcccattt ccgtttcgta
    13561 tatctcctgt tactaggact ttttctcctt tacttgtaca tgtcacgccc acattctgac
    13621 aagtaaggaa agaagaaatg gccggatcat cagacggata gtcttgtgtt ttatcaaagt
    13681 aagaaggttc caaggcagta gcaaaaccat tttttcgaga aataacgaaa gcatcgtcat
    13741 gcaagatcga aacagagtga ccttccgtat gttttgctaa ggtaatcgtt gattttccag
    13801 aaccggacaa cccaaataca gccatcgtat aggtctggtc attactagca tattgtttca
    13861 tcccaccgtg gcaggcaata aagccattgc gatttcccaa tgcccaagca agcgtcaaag
    13921 tcgctttttt taattctcca aagtaacgta atcctagcac agccgctgta ttatggagag
    13981 catcaaacaa aatgagtcca tttggaaatg tttcatgtgt ccattcggga tcacaaaaga
    14041 tataaagatc tccctcatca aaggaaacag acttttgata agtctctaag atcttcgaat
    14101 tcgctggctg aaaattcagc aaataagaat acaaattgtt ctcaaatcct tctggaatca
    14161 gcaaatgact ttgcacagaa aattcttcag ataggccaac gattactttt gtatggtaaa
    14221 actggcgctt gcgactttga tacacagctt ctcttaaaat tttctcgtat ttgctttgtt
    14281 ccttgtctaa gtcctcaaag atccgccgag cacttgctgt tcttcccaca atgcttccat
    14341 cattgaatac tagtgtccta acatcagctg gtaaatctag ttcttcagca tgtgagattg
    14401 ttaaatctgt gatgatcgtt cctggactcc cttttgctag ttgataagct gtcttcctat
    14461 caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat cgaatactgg
    14521 ataacagtgg atttccactt gtgatttcgt ttaattcgag actctctttt gtactcatgg
    14581 ctcctcctca ttccctaaac aaaaaaacgt tttctttatt atacattatt cgagttttta
    14641 tttgtactgc tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa aaccgagaac
    14701 ttgaataaac aagtttctcg gtttcatcat tttcctttta tcggcttagt accagccgtt
    14761 tgctaaccag aatgttttag cagcatccca tgaaccataa cgagatgtta catattgttc
    14821 tgctacacgt tcttgattgg cagctgaata gtcgccgttt aagtatgaag catctaattg
    14881 ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt ctttttgtgc
    14941 gatccattct tttgcagagc ttgaagttgg cgctgttgca gcagctgggg cagcaggagc
    15001 tacttcaact gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg tttcagttgt
    15061 agatgctgta ttttctgttg ttttgttttc tgttgttgct gaagaatcat ttgaagttgg
    15121 gattgttaat tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat ttttttcagc
    15181 aattgcatcg atcattgagt tgtctcctgc aaatttttgt gagatcttag ataatgaatc
    15241 acctgatttt actgtataaa cttcatcagc gtgagcattt gtagttccta caaacattgc
    15301 agtagcacca gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat tcatattaaa
    15361 ctcctttttt tctccgtttt ttattctttc gacgctatta actttatcat ttagaaatat
    15421 ttcataggta aaagaaacat gacatttaat gacaaaataa cgaattattt aatatttgta
    15481 acatttaaac aaatattttt ttgatacaa
//
LOCUS       DAZKCC010000066        15392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000066 DAZKCC010000000
VERSION     DAZKCC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.067"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..1073
                     /locus_tag="ACG8VH_002144"
     CDS             273..1073
                     /locus_tag="ACG8VH_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110689.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            1179..1967
                     /locus_tag="ACG8VH_002145"
     CDS             1179..1967
                     /locus_tag="ACG8VH_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110690.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            2199..3068
                     /locus_tag="ACG8VH_002146"
     CDS             2199..3068
                     /locus_tag="ACG8VH_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9110691.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            3088..3435
                     /locus_tag="ACG8VH_002147"
     CDS             3088..3435
                     /locus_tag="ACG8VH_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110692.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            3448..4710
                     /locus_tag="ACG8VH_002148"
     CDS             3448..4710
                     /locus_tag="ACG8VH_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110693.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            4790..6166
                     /locus_tag="ACG8VH_002149"
     CDS             4790..6166
                     /locus_tag="ACG8VH_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9110694.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            6163..6792
                     /locus_tag="ACG8VH_002150"
     CDS             6163..6792
                     /locus_tag="ACG8VH_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110695.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            6804..7274
                     /locus_tag="ACG8VH_002151"
     CDS             6804..7274
                     /locus_tag="ACG8VH_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110696.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            7271..7753
                     /locus_tag="ACG8VH_002152"
     CDS             7271..7753
                     /locus_tag="ACG8VH_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110697.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            7750..8412
                     /locus_tag="ACG8VH_002153"
     CDS             7750..8412
                     /locus_tag="ACG8VH_002153"
                     /inference="COORDINATES: protein motif:HMM:NF019467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP9110698.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            8399..8767
                     /locus_tag="ACG8VH_002154"
     CDS             8399..8767
                     /locus_tag="ACG8VH_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9110699.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            8848..9300
                     /locus_tag="ACG8VH_002155"
     CDS             8848..9300
                     /locus_tag="ACG8VH_002155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110700.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            10009..10608
                     /locus_tag="ACG8VH_002156"
     CDS             10009..10608
                     /locus_tag="ACG8VH_002156"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9110701.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            10624..11100
                     /locus_tag="ACG8VH_002157"
     CDS             10624..11100
                     /locus_tag="ACG8VH_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110702.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            11117..12019
                     /locus_tag="ACG8VH_002158"
     CDS             11117..12019
                     /locus_tag="ACG8VH_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110703.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            12092..12283
                     /locus_tag="ACG8VH_002159"
     CDS             12092..12283
                     /locus_tag="ACG8VH_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110704.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            12447..13034
                     /locus_tag="ACG8VH_002160"
     CDS             12447..13034
                     /locus_tag="ACG8VH_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110705.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            13034..13933
                     /locus_tag="ACG8VH_002161"
     CDS             13034..13933
                     /locus_tag="ACG8VH_002161"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9110706.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            13933..14847
                     /locus_tag="ACG8VH_002162"
     CDS             13933..14847
                     /locus_tag="ACG8VH_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110707.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            14899..15114
                     /locus_tag="ACG8VH_002163"
     CDS             14899..15114
                     /locus_tag="ACG8VH_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110708.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         5915 a         1981 c         3133 g         4363 t
ORIGIN      
        1 ttttgaatag agctagattc acttttaaat aaaatcaaaa ctgatcttaa ttctaaagaa
       61 aaagtcaaga aaaaaataca acaaattttt ttatcagaat atttatcaga atattattag
      121 aaaagataaa ctcaaaagct taatttaata gggttattgg ttgtatttag aaataatgta
      181 tggaaaagct ataaatcaat agataagatg tagaaaatct tatttgtgaa tgaaaaacaa
      241 gtcatcgtaa aatattaata ggaggtgtag taatggtggg aatatcaaaa acagctttag
      301 actcattagc taaactattt agtagcaggt ttgatggtgc agaatttgtt gaatatctaa
      361 gccaaaacga aagtggaaaa actctgatcg aaacaatgaa aaaagactat ttgaatgaaa
      421 atgactttcc gacatggacg aatattgaag atgctacatg ggatgtagta tgtacgatat
      481 attctccttg ggtaacgatt gctgattttg ttttagataa aattggaggg gatacatacc
      541 ctgatgccaa atctcttgct acaagtgact ttaaaacgtt ctatggttat ctaataggtc
      601 taataggcaa tggaatagat ttgaaagtta cgccaaaaca aacacctgaa gaaattgaaa
      661 aagaaaaagg agaggttcaa atggcttatg caaatgacag agaaaaagta aaattattgg
      721 aaggtaaatt atctgaagaa gaacttggaa aaatggtgca agaaatcaat gaagacagca
      781 actattttcc tgaattgaag gtatatcaaa atgacgaaga tttctttaat gaaatgttta
      841 gtaatagtcc atgggaagca gtgagtaaaa caaatttagg agaatggaac tttcaagatg
      901 atctagttgc atttagcaat gatgggtatt tacgatcata ttcagaaagt gagtatagag
      961 ctgaattaaa aacgtatgaa acggatattc tgatgagcta tttaaatcta ttagagagtg
     1021 gtcagggaga gttaagagat aaagttgaag aactattaaa agaagagcta tagggattat
     1081 ttcaacataa acacaagcag aaaactacta aacaaaacgt accatgcaaa aagtcggtta
     1141 aaccggcttt ttgctctatt atgagaggag atcaaaccat gagtgcaaca accgatatgg
     1201 ctttagaagc agtaataaaa gataatacag gaaatgtcga aaacgtagaa gactttagcg
     1261 atattgcaat caatctttca gcatggattt cagctgtagg agaagaaaat atcgaatttg
     1321 agatcaagaa tatggatgac ttaaaagcaa aatttccttt tatcgcatcg aaactaagtc
     1381 aagttgccga ggataaatcg tggaatgaat tagttagttt ttccgaaaaa atttatgata
     1441 ttacgcaaga agatgagtgt gtttcatggg aaatgaataa tcaatacaag aaaattgacg
     1501 ctttcgtcaa atcacgtata gaaggaacag cttttgagtt tgaaggtata gataccgcag
     1561 ataattggtt aaataaagaa tcctttatta aaagtatagc ggatgatctt gaaaaacaat
     1621 ggtctaagga agactatgca gagtacctaa aaacagaaag agatacagag gtgaaagaaa
     1681 aactcactga ggtatgtttg gatgaacata ttgatcccaa tatcgtaaaa caagaagact
     1741 atgaagtggt tattgataaa gttcctactg attttgagga agttgtaaag tatctagttg
     1801 aagaagcatt atcgcaatac tcttcagatg aggaagtgga cgaagaaact gttcgtacca
     1861 gagtttggca actggttttt gatgacaaag attataaata tttgagtgat caaaactcgg
     1921 ttaattggag tgtacgatta ttaattgatc aaaatgatat tgaataaaat agagctgaaa
     1981 agctctattt tttttgttaa aaaaactagc tattttatta aaaataattt ggttcaaatt
     2041 ttattttttt aacttgataa tttcttgcca tcctttttta aattggctat atatcaaggt
     2101 ttatagtggg aataaataaa aatacatttg acaaaattta caatatagtt tattatatat
     2161 ttatcttatt atgataacac aataaaagga atggtggtat gaaaaagaag aagtttagtc
     2221 gatcgttgtt aggaattttg gttttagttg cggttggtct tagtggcaag ggttactttc
     2281 agaataacag cactcaggaa catgattttc agatcaaaag tagtgttcag ataggtaata
     2341 aagcagacag tgaatcaaaa gaatttttag ggatacgtta tgatccaaac aaatatccaa
     2401 ataatatcgt aaacattaat gatgggaaat atacactgac aaaagaagac catgaagcat
     2461 tatcagaaaa aggcagtcaa accttttggg taaattatgc agatttagat caacttggaa
     2521 gagcaggaga tgttgaagcg ttgatcacct atgatgctgt aaattctcac tcatctaaaa
     2581 atgtggaacg tccatctttc aaaagttctg tccatattgc aggagaatac aaggatggag
     2641 tatttgatca agagagagat acttgggtcg gcaaagattc aaacaatgaa atcactcagc
     2701 tagaaggcta taaaggttat ctctataaca aaagtcattc attagcatgg tcgctaggag
     2761 gagatatgga ggtacacaat ttaacacttg gaacgagagc gcaaaatgtt gggaaaaatg
     2821 atcagaatgg agggatggct tactcagaaa cgattgtacg tgatgcaatc tataaaaata
     2881 agaatgaaaa agtattgtat aaagttgatc caatttataa agggaatgaa ttagttcctc
     2941 gtggttctca tgtcgaagct ttctcagtta acgataatgg aaaaacggta aaccttaatg
     3001 tttatgtatt taacaatcaa aaaggaatca acatcaacta tcaaaatggt acttgggtaa
     3061 gagattagaa attgagaagg tgaagaattg gaaacaataa gcatcaatgg attagataaa
     3121 tcagttgcaa atcgaataaa aaaaggaaat ggctactatc ttctgaagaa gggtaaatat
     3181 tcagttgcta gagagttaga aacaatgagt atcgtgtatg tagctaagaa tcattttgac
     3241 gtagaacagg ctatttttga tccagattct gaaataggga aatgtgttga attatcagag
     3301 aaaactgtta gtacaaaagg ggcgatggtc attgagagag aaatgttaga cgaaatcaga
     3361 gtaatcattc ctaaagatac gcagatgtgt gaggacagca taagtgacga tgaaccaaaa
     3421 tcagaaagaa aataaggaga tattcagatg agcgaaaaat ccattgaatt aaaggaatat
     3481 aagcgaagcg atggagaaaa atattatcct gtagccagtt tttctaaatc agtatatctg
     3541 gttggacgtg cattggtagt tggtgcaatt ggcatatata ttttgcattg ggtaacaaag
     3601 gttgtacaac tgcttgtaga taccttagta agtattcttg tctcgttaaa tgatgttaac
     3661 ggaacagatg atttaggatt aaaagagatg tttaaagagt tactcaatgc gttttttccc
     3721 aattatttaa gttattcata taatgtacaa agcttttttg caaagctaca cttggtagac
     3781 atctctattt tagtattaga agcaggaacg gtattctttg ttggatattt ggttactttg
     3841 atgttcaggc atcactatgg agaacaaaga ccattttttg atgatatgga aagtagaagg
     3901 ttgaaaagag aagctctaga agctatggat gtgaagtatg atccaaacaa gattatagga
     3961 gatgaaacga acaacaaaaa aagaaaacga aacaaaacgc aattagaatc cgatgtgcta
     4021 tcaacgctta aaaatatcaa aatttctgtt catacaagac gtgatctaag tgtcaatgaa
     4081 gatattcgag aatatatcgt aaaaatcccg caaccaaaag ggaagaaagt tagagaaaca
     4141 gttttacgac aaattcaaga tttagggtcg atcctgacaa gagcaacaag cggacgagcc
     4201 aaatttggtg aaaaagaaga tgaagtttca ggcgattatt tcattttccg agcatcagta
     4261 aatattgaaa gagaacgtaa ggaaagagta agtaccaaaa acaaagaaac ggaaagagca
     4321 acatcttcaa tcgcagtttc aaattcacaa ttttcttttc caatcaatct ctttgccgat
     4381 aattccaaaa atatccaaaa ccaaaccgaa aaagctagaa aatatgccga aactcaaaag
     4441 caatctttag aactttatct atcttctgct gacgtacctg caagtctaac agatatgcac
     4501 gttgggaata catctattga atatttgttt actttatctc gtaccagaaa aattcaacta
     4561 agctcaatga aagaagaaat tgaaacttac ttgcatatat caggtgtaat cgtgcggatc
     4621 aacgcagggc gtattcaaat tactttacca ttaggagaaa atgatcgtat tccaattgat
     4681 gtaccatcaa tgatcaatag cgtgttttag aagacatcac aaatagaaac agaaaataca
     4741 aactaccagt tcacaaacag aaaagtaccg agaaaagtga ggaatagaaa tggctatgaa
     4801 attattagag aaagtcaaaa agagagccga aaaagctgaa tctattttgc tagataaaga
     4861 acctaatata aagcttaatg gaaaattcag tgtcaatatg ttgaacacaa aggctaaaga
     4921 cccaacagat gctatttttg gaattaagcc tgatggcaca attgctcatt tttgcatcag
     4981 cgaaatgcca cacatgttgg ttgcaggaac gacaggatca gggaaatcag tattactgaa
     5041 tgaaattctt gttacagtaa tgtgtcactc aacgcctgat gaattaaaaa taggaattgt
     5101 agacccaaaa agggttgaat ttggaagata caaaaaattg ccgttcatgt tggctgatcc
     5161 aattacagat atggatgaag cttatgattt ttttgaatat cttgtgatct tgatgcatga
     5221 acgttacaaa ctgatggaaa aagcagggtt acaaaacatc acgctatata acgaatatgc
     5281 cgaaaaaaat ggattagata gatttcccta tgtgatctta ttagttgatg aatattcaca
     5341 gttggttgga acacataaag aagtagaagg tttgattgtt caattaggac aaatggcaag
     5401 ggcagcaggt attcatgtta tcttagcaac acaatctcct cgatcaacag ttgtcactgg
     5461 tattatcaaa gccaattttc cgtcacgtgt ttgccttatg gtagcaagtg atttagaaag
     5521 taggattgta ttagatgaag gcggtggaga aagtctaagt ccaaagggag atatgcttat
     5581 taagcttgtt gatggaagta tggttagagc gcagggagct tacatcagta acaaagaaat
     5641 tgaaacaatc ttcaatcacc ttagaaatac tatgccagaa ccagaatatg ttgattaccg
     5701 agcaatcgta gctgaaactc gtggtgaggg cggtgaagac gaggattttg gtggaattgg
     5761 taaagctcct gtaagaaaat ctgctaaaag aaatttgaac acacctttag caaatccatc
     5821 atctaaagag ccatcaattg aaaagaagag tgcgtttgct attaagcttg aaaaaaataa
     5881 agaagaaaaa gctaatcagg gagctaagtc actagaaaaa cttcagcaag aaaaattaga
     5941 aaaactagaa aaagcattat catcatcaac ttctgaaact caatcttcaa agggtataga
     6001 taatggacaa tcaattacaa ctaaaaacaa gcctaaaaat agtaattcaa atctctcggt
     6061 aaatgttgtt atcacaactg ataaatctga aaaaaaacaa gtgaacggaa ctacttcacc
     6121 catcatgcaa aaaatgataa aggcaaaaaa acaggtgcag aaatgattat cattagttcc
     6181 tttttgagat ggctaacacc atataaatat acagatgaaa tagttgagaa gcgagcgaag
     6241 aaaaaagtta aaaaaacgat gttaactcct aaagaaatgc tagatcgtgg aatattggga
     6301 gtgaaggttt tagaagtaaa ccaaccgttt gaattggata atgcgagaga taggttgctt
     6361 agagctgaag ttgatgtcgc atacttttat catgataaaa gcaagcatca aatagctatc
     6421 aaattcaatc caaaatcggc atggcgatac caagcaagag atgacctaaa gcgtaggaag
     6481 ctgaaaccga tgctctatcc tgaaggaaaa gaggcatttg atagaaccca tattgttccc
     6541 attggatttc atggtacaga gtcagataac cgcctattgg tagggtggaa cagctcacaa
     6601 aatagagttc agatcaataa atttgagaag aaggtaagaa aatttaacga tcggaaaata
     6661 atactttggt ttgccaatat tcagaagcaa ccagatggga cagcaaagtg ggaagcggtc
     6721 atatttgatg aagatggcaa aaaagtaatg tctgaagtat ggcatgataa agctaaattt
     6781 gtttggcagt agaggtgaaa attgtgttaa ctaacctaaa actatataga gagggattta
     6841 acgaaaaagt aagtatcggt gaatctattg aatatttagg aaagaagtac attattattc
     6901 gtattcttga aattaaaaaa ctttatttta gtaaatataa taatttagca gttagagttc
     6961 tcgtccaaaa ggttggaaat ccaagtgact atagaagtga ttaccataac tataaaacag
     7021 aatcatctgt aattaagcgt tataatcaat caaaaaatga tgagagaata ttcaaagtag
     7081 gagaaattat tatttctaag tatgggatag catacgaaat agtttctatt aaaaaatttt
     7141 attatgaatt tgttgatttg gttgtagaat attctgttcg attgatcgtt ccgtggagta
     7201 aagaagaaat taatgaggca ttaaaacatg aacgaaaaag tacattcaaa gttttagaag
     7261 gtgaaaaaaa gtgaaaacag accttactag gcaagctgag aagtgcttat ggcatgaaac
     7321 aaatcgcatg ggtgtatttg gttgtttcga agtcactatc ggttgggatg gcagtgaaag
     7381 agtagattat ataacttatt cagtagataa cacagttcga tgttatgaga tcaaagttac
     7441 tttagctgat ttaaaaagca aggcgaaaca gacttttatc ggtgattata actatttagt
     7501 tgttactagt gatttgtggg agaaaatgca acaagacgat aaacttatgc ggaaatatta
     7561 ctatcaaggc gtatacgtgt tcaatgaaaa atatcctagc ataataagtg ttaagaaagc
     7621 aaggaaacaa aaagtatcgc taggtacaag atcaactgta ttagaaagca tggttcgttc
     7681 tatgaatcgt gaagttaaga aactctataa agtaaaacca ttttgggaga tagcagaggt
     7741 ggaaaaagga tgattcaaac atttgcaaaa atgagtagaa gtgcttggat tgaaaagctg
     7801 gaaaatgatt tgagtgttat gcgattagct ccaaagaaat tcaaacgaga gattgcagaa
     7861 aaagaagcaa ttttgtcggt attgaatgcg gtagaactgg acgaaccgaa aaagtcagtt
     7921 gatgtacctg aaaatgtagc agagttacta gattattatc gtaattcgtc agatgtcgat
     7981 ttgttagctc tgatattaac atttcacgat tggtattact gtcaaaatga agatactcag
     8041 catgataaac atgataaagc agttgactgg ttaatagaga atccagaaaa gttcatgcgt
     8101 gcttggctag atggctatga tagcaagaaa aagccactgg aaagtaatga ggaaacgtat
     8161 ttaacggcag aacaatatga ctatgctaga aaaacaggag attgggaatc tggtaagact
     8221 ttcaatcaca gaaaagatca accaaaaatt agtttttcag atatagccgc aattatcgaa
     8281 ggtatctact atggacaagc cgatgaagag atcttttcta tacaagattt attagaattt
     8341 ttgaacaaaa ataaggatag ctttgtagaa ctaactgatt atgaggagga tacagcaaat
     8401 ggaaaattat gatccaaata tgcgatgggg tacacatacg cttaaagttt cttttcaacg
     8461 ttgggattac aagggatttg tgacttttag aaaagctgga aattgtaaag gattagatgt
     8521 cttagcgttg gatgaagact atctatatga ccatccattg acggataatc caatcggatt
     8581 tggattgctt cctgaagatg atgagggcaa tgaatggttc aaaatgattc ttacaaacga
     8641 taaaggtgat caattatttg tagaagatac ttggtcatat ttgagtgaat atattgttag
     8701 cattaaaatc attgattttg tagcagataa agaaaaagag attggagaag gtaaatctaa
     8761 ttactagcta atgaatagca actttggagg ataagtcaaa agaataaaaa aagaatactg
     8821 tgtgtcattg cagggatgta tctttgcatg atagtatctg cggttaccat ttattggtta
     8881 ggaaatcagc aaaacagttt aattgttgat ctgggggagt taaaggttgg attacgaatg
     8941 actagatggc ttttattagc tcaaacagtt ggtttagcca tattgacttg gatatgtgtg
     9001 gaaaaaataa catctaataa taatcaagag attgttgaat caacaaacct aagaataaga
     9061 ataactgaac aagcaattga aaagcaaaaa gacaaagaaa aaaaggaaat aatgtcaatt
     9121 tatttatcta gtggagcatc attttttcat gaaatagatt cgatgaaact gagtagtcaa
     9181 gaaatcattg atagatggaa aaacatgcct ctaggtggtg agttaatcgt tgaagatagt
     9241 gatggttggt cagcttataa gaaggacgag attgtagcta tcacgatttt gtttgaatag
     9301 taatatatta ccactagata atctgaatat gttgatctgc tagtgacgta ccaaatgcgt
     9361 ttaatagaac cttggtcaca ggcagaaatt gataaagcag taaaattgaa ccgattatca
     9421 aaatttaacg tattaggaaa tgccttttaa aaatcgcaat acacaaacag aaaaatacca
     9481 aaaaattagc aatgaacgta ggtaagaaca tgacaataaa taataataac cctaacaaat
     9541 ataatgctct tgtccaatgc tatatcgaaa acaaaattac aatgcaagaa gtcgctgatg
     9601 aattaggcaa atcaaaaagc ctagtatcac acgactttaa gcacaatgtt tttccgaacc
     9661 cgattgagct gaggaaaaaa tttcaaaaga gagtttatca agaaataggg aagtggattg
     9721 atgaagggaa tgcaattgac cctttaattg aaaaatatcc ttttttaaaa agtaaaaata
     9781 aaattcaaag gttaattcgt caaggcgata ttcaagctaa agaacctgtt ttgacaacca
     9841 atgatttggc taaaataaat cttcataaac agattattga agactttgaa aaaaatcttt
     9901 caacaactga aattttaaaa aaatacaata ttccgagagg aaagctaaca tcgactcttt
     9961 ttagagaata tggagaatcg gcagttacta aagcaaagga agtagcgaat gagagaaaaa
    10021 aacaaaaata gtagtcgata cctagtagcg actgtagttt ttgtttgtgt attgattttc
    10081 ctgtttgatt ttttgcagat tggaaaaagc ataattggaa atacattgag tgcaaaggaa
    10141 atggttgctt taggtggttt gtatgaagga catttgttcc cgaatatatt catttctatc
    10201 gtttctcata ttaacttgta ccatttaatt tctaatttat tgttcttagt agttcttgaa
    10261 gaagcactga atgatcaata tgagtggtta acactattga ttgttttaat catatcagga
    10321 cttctaggaa gcttagttcc ttttctgtta caaagcaaca tcgtaagtat ggggcttagt
    10381 ggaggtattt ttggatggat tgctttattt gcgattgcat acatccgctt tgatagtaag
    10441 gaacatttat tactaagtgt ttttatatta ttgataatcg gcattttact ggtatctact
    10501 tttgaatcag gcgagacaaa cgtcttgtca catgtaatcg gatttgtctg tgggatgatc
    10561 ttgggattta ttctaaagaa catttcattg atcaagcaaa agaattaaaa gggagtgcta
    10621 aaaatgccaa aaaaaaaaga aaagaaaatt ccagtgtttt tgtctgtctc agaaatgtta
    10681 tcacttcaaa tactgttaag aaaaaagatt gaaacaatag acgaagaagt tagaaataaa
    10741 agggctatgt acgaacaacg tgaagcgcta agagaacgaa tccactattt tgatgatata
    10801 cacactaaaa tctatgaagc acgtacaaat aacgaagacg aaagcaaatt aggattagct
    10861 caacaacgaa tatttgaatg gctaaatacg aatactgagc aagaaccgtt aagagcaatt
    10921 agtaatcttt ttgaatttgg aacgaagcat tggtttaacg cagtagaaaa ggatgctgac
    10981 ttacaagaag cctttgacac gctaagtaag gatagcaaat ccatgcgaga tttattgatt
    11041 acttatctaa atgagcatga gtttgaacaa acgaaaacca aaaagaaaaa taaagaatag
    11101 aaagttgtga tgaatcatgc tattaaccct gttaggttat ttatgtttaa ttcttgcttt
    11161 ttttggggca atcgctataa ttattggtca atttcaaaca aataatccga ttatgcgtaa
    11221 cataggcatt ggaattgtga ttgtaacctt tattgtgttt aatgccacta agatttttgg
    11281 aagtaacgat aaacaatttg gtatatcggg agttaataat agttatgctc cagattcttt
    11341 gggaagggta gtaatagatg gaacatttga aaatgtaacg agttataaaa atgtttcgct
    11401 gacagtgaag gaagatggca agaaagcatc agacgacgtt ctcgctacta ttaagccgtc
    11461 tcaatcacaa tttgaagtgt tttatacagt taaagagaat agccctgtac acaagctgac
    11521 attggttttt aacgttgatg gagaagaata ctataaagat gtaaaaataa ataaaacatc
    11581 aacgaaaaag attatcgaat cagcgaaaaa agaagtagct acagaagaaa gtacaactga
    11641 atcaagttct actgtcgaag cggaactacc agataaaatt gattttgatc ctgaaaaagt
    11701 caaatcgttt gaatctgttt ctggttctat ggctgatcgg tttaacactg aagtcttcaa
    11761 agctatcaaa actgttaaat tggatactga aaagcaaatg attaatgtta ccttattcaa
    11821 ttcagttgaa tctatggatc aggataaaaa agttacggtt gcaagagaac taggaaaaag
    11881 agtaattggt ttgtatcgtg gtactgctaa accagattca gacggtgcag atttgccagt
    11941 tcatgtagtt tatgaagacg gtaaagtttt tggagattct acagcaaacg aagcattaac
    12001 gattaaattg gctaaatagc cagaaagtta taataaacaa atggtgtcac accaataata
    12061 tataggtgtg acaccttgga gatgatagct gatggtagta aataaagaaa aagttagaaa
    12121 gtatgtgact ttaaaaatag cttctgttaa atatttagaa aagcgagcta aagaagaaga
    12181 aagagcaaga ggtaagaaag taacagtttc agagttgtta gaagaattga tcgagaatga
    12241 tcaaacaata agacgtgcat ttactaatag gtctatcaga taacaatata attaataaaa
    12301 aagaaagagt gatttggtct tagactaaat cactcttttt atataaagtt tctttacact
    12361 cattccgaat aatgttaaaa ttctttatat gatatatagt tccttgtcag aggaagagat
    12421 aaccagcaat attggaggga taataagtgg aaaacaataa tgatattaga cattttagca
    12481 ttggggaaag agttacaaca gtaagtgaat tatcaaagga ttatagagat aaagagggaa
    12541 cgattatttc tcttaataag aatcgaagag gcgtttggca atatgttgtt gatttaggtg
    12601 atggcgttac agttgagtgt aaagatagtc aactgatcag aaataatggc agtcattcca
    12661 ttgaaaaagc aattcttgta tacccaaaag tatcaagtaa gatcatgaat gactggttgc
    12721 ttggaaaatt tggtgagtac aataaaaaag aaatcgcaga agtgatcggc aaatcttatc
    12781 caacagtatt agggtatcta aaaaagataa cgagtaaaga agcaaaaatt gaggatgttt
    12841 taacagtaag agagcttcaa tcattaatcg agtatcggaa taaacaaata acaaatcctg
    12901 agtaccttag aaaagaagat ggtctattga aacaattgga acaagtaaat aaaatccgga
    12961 agatgcaaaa ggtagctcct ttaagccttg atacctttaa aaaaatctat ttgaaagaga
    13021 tcaaaaaaga ctaatgtgga ctgtaacttt accgttccaa cctgtatctg caccaagacc
    13081 aagtgcaaga aggaatgctg ctgatgaaac taatgacgta gcagagaaaa ggatttcaac
    13141 ctactatgat aaaaagtatc ttgattattt gaaaaaaata aagagcttta tcgaagatca
    13201 agatttatta gatgatgaat tttactcgat tgtaaatgat cctatgggcg caattatgga
    13261 agtggacttt tactaccaga taccaaaaag ctataaaaaa gtctataaca ttatgaagac
    13321 aacagcgcca gatttagata atttagtaaa aagtgctatg gatggtattt ttaacatctc
    13381 agctatacga gatagccata tcacaggctt aattgctttt aaatataatg tggcgaacga
    13441 aggaaaaaca gttgtgagga tcaaacgata ttcagaattt gagtgggata cttctgaagg
    13501 attgaacgga gatatatggg aagctacatt tgattttaaa ccagtagcaa cgccaagacc
    13561 aaagtctaaa tttagaggaa atgggattat cacttactat ccaaaagagt acaacgacta
    13621 tttggaaaat atcaagaata cgcttaaaga gaaagatttg gtaaatgatc aactgaaaaa
    13681 ggtaatgaat gctcctatgg gggttatagc tcagatagat tatttttacc aagtgagaaa
    13741 agataagaaa aagcttgatt cactgatgcg tacttcacag ccggatattg ataatttagt
    13801 caaaggaaca ttggacggaa tttttaacaa ggaaataggc atcaaagata gcagggttgt
    13861 agggttaatt gcttttaaat acaatacttt tgaaaagtcg aaaacaaacg tgagactaca
    13921 ggagatgggc tgatgaataa taatgaaaaa attcttcatg tacttgatag ctttgaaata
    13981 attcaagaag aactaaaaaa atatcgggat gttttggaac aaagatatga ttttgtcaat
    14041 cagcaaaaga gtaatcacat ggatttcatt ctcaatatga atgacttgaa gaagaaatta
    14101 gttgaacgaa aagaacaaga aaagttgatc aaagcttact ttgaactcgg tgaaaaagaa
    14161 gttaaaaatg caatggaact gaatgaagag cgcagggttt tagatcagct gttagaacaa
    14221 ttattggtga tgtttcaaaa aggacgaata gatgaggatt taattgaaga aggattgaga
    14281 aaatatcctt caaactcagg tatcggaata gtgctgaaag cgattgatga agaagaaatt
    14341 gaagctttca taccgccaga ggattttgaa tcggcaatgg aatatataaa atattatagc
    14401 caagggatca ctgcttttag agagtttgat cctgaagatg ttattcatga cttgaacaac
    14461 ctaaaagaat ggtgtgaaag ttatggagtc gatgattccg gattggacta cttgatcagt
    14521 attatggaaa tcgaagaaga gatgccggat aagcctgatc caacagatat tttagaattg
    14581 atccatgaag ctagaaatcc aattgcctat atttctagag gatatactgt attggaatac
    14641 tataagcctt atatatcagc gatgaaccat ttaagacgtg tattaaggga aaaacgtgaa
    14701 taccgctcag tgttgaatgc aacgaataga ttagaaaaag ctgtttcaga gctagatgct
    14761 tattatcgtg aacattacct gcaagcaggt ggtatgccaa gaaatacgaa agcaaatatc
    14821 agtaggtata taaaaaaagc tgagtaggcg gagttagccc ataaaatggc aaaaggtaac
    14881 ctgaaagggg cattttgaat gaaaagggat gtttttgaat tgtacgtatt agaacgtcta
    14941 gaagacggca tgatggcaca agagataatt gatgaaattg atgatatgat cagcagattt
    15001 ggtcaattgg agttgaatga tggaaacaaa aattcggtag tgctaaaaca attttggttg
    15061 aacaattttt cgtataagga ttttatcaaa gaaaaggcaa atacattgta ctgactaaat
    15121 tcacgaggag cagtgatgag agcttattgc taagagcagt taggctcttt tttgtttgtg
    15181 taaaattatt tgttttataa tacacattta tacacaaaaa aatattgaca gatagtacac
    15241 aaatgtggta ggatatattt gtaagcaagt tagattaaca agttaaagga agagcagtta
    15301 tgaaaaaaga agaactaata gatatatttc aaattgttga acgagcaaat aatatgggaa
    15361 ttatgttttt tgatcgtatt tctttaaaga tg
//
LOCUS       DAZKCC010000067        15381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000067 DAZKCC010000000
VERSION     DAZKCC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.068"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..857
                     /locus_tag="ACG8VH_002164"
     CDS             54..857
                     /locus_tag="ACG8VH_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9110709.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1442..1897
                     /locus_tag="ACG8VH_002165"
     CDS             1442..1897
                     /locus_tag="ACG8VH_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110710.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2016..3074
                     /locus_tag="ACG8VH_002166"
     CDS             2016..3074
                     /locus_tag="ACG8VH_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFP9110711.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3116..5065
                     /locus_tag="ACG8VH_002167"
     CDS             3116..5065
                     /locus_tag="ACG8VH_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9110712.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5068..8709
                     /locus_tag="ACG8VH_002168"
     CDS             5068..8709
                     /locus_tag="ACG8VH_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFP9110713.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            9109..9447
                     /locus_tag="ACG8VH_002169"
     CDS             9109..9447
                     /locus_tag="ACG8VH_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110714.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            9533..9778
                     /locus_tag="ACG8VH_002170"
     CDS             9533..9778
                     /locus_tag="ACG8VH_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110715.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            9798..11954
                     /locus_tag="ACG8VH_002171"
     CDS             9798..11954
                     /locus_tag="ACG8VH_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110716.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            11966..12199
                     /locus_tag="ACG8VH_002172"
     CDS             11966..12199
                     /locus_tag="ACG8VH_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9110717.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            12189..13055
                     /locus_tag="ACG8VH_002173"
     CDS             12189..13055
                     /locus_tag="ACG8VH_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110718.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            13070..15040
                     /locus_tag="ACG8VH_002174"
     CDS             13070..15040
                     /locus_tag="ACG8VH_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110719.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         5709 a         2175 c         3101 g         4396 t
ORIGIN      
        1 gtaccagatt aacatgatta aaggagcgca aaaagaagat tggagagtaa tcaatgtctc
       61 aagaagttac cgaaaagatt attgatagtg atgtagattt agtatcagaa caaacaaatg
      121 caattgtgcg ttataacaat ggtctgaata caacagcttt gcgaggattt aagcctgttg
      181 aaatggatgt gttttgggct attttatcaa aaatgaaacg tcaaggaaca gcagaagtaa
      241 cttttgattt caacctcttt cgttatttgg tcaaatacga cagaagagaa aaggacagtt
      301 tttattctgt gttaaaagat ttgagtgaca aactaggtaa tttaacctac cgttttgaag
      361 attcagacgt ctatgaacag ctgtggcttt ttcaaaaatt ccggatagat aaacagaagg
      421 aaacagttac aattaaggca aatgaggaat ttgaatttat cttaaactca attggcaaaa
      481 actttactcg ttttgaactt gataatttta caaaactaag aagtagctac acaaaagaat
      541 tgtatcgtca tctcatgcaa tacagaaata aagagtggcg aaatggttat tgggtagtca
      601 aagttgaaga ttttagaaga gcattaagta taccttcaag ttatagaatg acaaatattg
      661 ataaaagaat actagagcag gcgaaagaag aatttttagc tcctgatgaa aacggaatac
      721 aggtatttga aacttttgat tatgaaaaaa tctatgcaaa gaaaggaaga agagttgatc
      781 ggctcagatt cacttttagt gagccagaat caaacttgcc aaccatttca atgcataatt
      841 ggttagaaga agattgagaa gaagcaggtt gtaattttaa tacaacctgc ttctttaaaa
      901 aagtatgggt aacacttttg gacaataaat gcaagaagtt tttttgtaag caagtttcag
      961 tctaaataat atttgaaaga atggctaatc tatacaaatt ttaacaacta tgggtaacac
     1021 ttttggacaa gtcaagaaaa aaaacaattt ttcttaattt gttgatcgac taaaggtaac
     1081 acttttggac aacacagaat aaaaagtccg gtgatatcag ttttggtagg aaacatctta
     1141 atatctttgt gagattgaag gtaacatttt tggacaagtc aactatttgt taaaagttaa
     1201 tatagagtaa ttagataagt tttttctttt cgtctgataa atggataata ttagttattt
     1261 tattgctaat tagtctttca tgatgactga aatagctaaa ataaatatct cgttggataa
     1321 ttgctgatac tttaagggat atctaaattt gctgattgat ttatttatgt tattatgata
     1381 ttataataac ataaatgtgt tacgcccgaa cctcatttta aaaaaatgaa ggggtgagac
     1441 aatgaatgct attaattggc atgaatttgt taactatatg gagagcttac tagatgatct
     1501 aaaaagtttt agtattgctg atgcagtaaa gcatgggtta ccagttagta caacgatgaa
     1561 attacgagga ttgaaaattc cagatgaaat tattaagctg agaactaaaa caattattga
     1621 aaccgcaaaa gtcgttgaca atatgaacca tgaactaaaa ggcaagaaaa caacttacga
     1681 gaaagatttt gaacgttttt taaaatctgt tattggaaaa tatctaggta aaaaatcttt
     1741 cagtgaattg agagatgaag agattgtacc tactccaatg gttagcaaaa tccgaaaaaa
     1801 caaagccgag gggaattact atcttggtac aatagcaaaa atcattgatt atttggaaaa
     1861 taaagagcaa aagaaaggaa cgaagaaagg aagataattt ttgtgttaaa tattaatggg
     1921 gaagaatata ccgaaatgat tcaaaatccc gaacctagaa ttaccgaaaa aacgtctgat
     1981 tgtttgctct tttatgaaag ggggcagaca aactaatgga aaaaaacgtc aattcaacat
     2041 ctaccaaaga tcaatacagt attattggag ggttgcttgg tgataaggat aagagaacac
     2101 gtcacggatt acgcattatg cttgcaactt ttctaggagt gcttttttta ttaacaggct
     2161 tgggtagcta catttatcaa ttaggaaact ataattatta tctcgtgaac aatacaacac
     2221 ctataggctc ttcattggag tttgctcgat caggagcaga gatcactatt tcagatgtgt
     2281 ggacagatca aaacagagat gtaacagtag taaaactcgg ttactcgcaa aacgctagaa
     2341 agttgttatc tacgcaagga aaaaactaca acttatatat ggcaacgaaa gaaaaagatc
     2401 gtcctaaaaa gcttgaaatg agctatggga tgttaagtac tgatggggat gctttcttat
     2461 tcctaaaagg taaattagat gaaagagctt atcaagtttt tattgcaaac caagtatcac
     2521 taatttctgg tgatgataca gctgacgatg ataccaaagt caatgtgaat gaagcagatt
     2581 cagttacaaa agcactttct caatattcta tgaatgatgt taataatgaa ggagtaattt
     2641 cttttgggaa aaaagcaaaa cctgttaata ctcctgataa tatcaatttc agaataaatc
     2701 catattcaga ttcaactaat gtctatcaag gaagctttct taccaaagat ggcgatattg
     2761 attattcaaa agttgtagca gttacaagta agaataatgt tgtaagtaag ttagaagatc
     2821 agttaaatgc ttcaaatact aacatcaaga aaattgaagc tacaaaagct gagtatctgg
     2881 aacgtttaca acaaaatcct gacgataaag attcgcaaga aagtattcgt caactagaca
     2941 gtcgaatgga gaccgaaatt aaagcaatca atgatacaca aaaattgatt gatcaataca
     3001 aaaatgctga ctttaacaaa gattcttttg gagacatgaa gacgacattt aagtacctaa
     3061 ctctgactga ctagaagtaa attattctta ttttagagga agtgaacaca aacagatgga
     3121 aaatagtacc gagtttttta agaatattca aactgaattt aatcatgctc tcacaccaat
     3181 gaaaccaccg aaacataagt tagtgggacg tgatgacgaa ctccatgagt tgtcactgat
     3241 cttgaataga ccggaaacac cgcaagctat cttgttaggt gaagccggaa caggtaaaac
     3301 agcacttgca agtgaatggg ctgaaagaca gcgacaagat ggaaagaaag tacaagtata
     3361 caaattatca atcggacaat tgcgtgttgg gggagatgat gtcctccaaa cacgtttaga
     3421 aacattaatt ccaagattga aagaatatga gcgaatttta aaatctgagg acaaagaaaa
     3481 tgaatttgtc ctcttcattg acgagtttca taaagttgtt agctcttttg gtagtggcaa
     3541 taaaattggg ggtgattcgc taaaagaacc gttagcaaat gaatcacttc aattgattac
     3601 cgctactaca agaaaagaat atgaaaacta tatcaaaaga gatgatgcct tagggagacg
     3661 gttacaaccg ttagaattga atgaactaaa aagcgatgtt gtcgtcgata ttttgagaca
     3721 atggctaaat acatcctctg ttaaagatga tcctcaggct tcttttaaat tatcgcaaag
     3781 gattcctgat gaattacttc aagaaatcgt acaagcaaac aagctttatc gtgaggattt
     3841 gtccgaaccg gctaaatcaa ttgccgtaat ggaggatttg attgcaaaag atcgagtaga
     3901 cggaaatgct tataatcgag aaagtctatt gcaaatattt ggtaagtata atattcagct
     3961 agattctgat tttgatatgg aaaaacttca agaggaatta aagtccataa aagggcaacc
     4021 gttagcagtt tatactataa caatgttgtt taaacgtatg aagcttggat ttgatcgttt
     4081 taaaggtcga aagcaaccaa aagcaactgc tctgtttaca ggatcaacag gggtcggtaa
     4141 aactgcaatg gctaaagccg tggcaagagg actttatggc gatgaaaata aagttctctt
     4201 agttaatatg ccggactaca aaacaaaaga tgatgcaatg cgttttagac gactcatagg
     4261 tactcatgta aggcatgatc cttctgcgat tattgtgtgt gatgaatatg agaaagctgg
     4321 tgtagaaaca ctcgatgcct ttttatcaat tttagatgaa ggtaggataa gttttactga
     4381 aagaggatgg gatgatcgag aagttcacga cactgtttca ttgtcaaata caattttatt
     4441 tgcgacaaca aatgcaggtg cggagatgtt cgattcgtta aacagatata gctcactatc
     4501 tcgtgttaca aacaaggaaa attctaatga atatagcact gaactaaaaa gagaataccg
     4561 aaaagtcgca tcagggatca aacaagcctt gattggtgaa ggtctaaagc ctgaagttat
     4621 taaccgattt ggacgaatag ttccattttg ggctttatcc gaacaaactc ttctggaaat
     4681 tgcttataat gaaatgttga agagtgctga attagtacga aaacatttaa atatagattt
     4741 acgtcttcca cctaggaaag aaagagtata tggtcaaaat ttttatgcag acgatctctc
     4801 catgcactta gtttttgaca tggcttcaca agaggattct aaatcagggg gtgcaaggaa
     4861 tatcttgcat ttagttgaat ctgagtttga agaagcgatt actgaagcaa taattactta
     4921 tccaaacgca aaaatattcg atgttaagtt gagtgaagcc ggagcgtttg aaggaaaagg
     4981 tgattctaat gctgaaatta ttgcaaagcc ttccgttgct gaaagtaaag aaaacaagat
     5041 tgtattttcg attggagggg cgtagctatg aaattcaaga aatatttact acaaaacctt
     5101 agaataaaac gatgggtcgt aagaggtcta gttattggag gagtattagc agttacttta
     5161 ggcgctcctt ttggaatgaa tgatccaact cctgacaatc aagatagcaa acaagaaaca
     5221 gttataaata agaacgttgc atatgcggaa agtaaaccgg cagaacaagg aatgtggaca
     5281 ctatatgctc gtatcctaat gggagataaa gaagttaatg atagtgccgc taaaactgga
     5341 caaatgaatt tgttaggaca aggtggtatc agtgccgatt tcccttattc tgacatttct
     5401 aaacaaggta attcgattgc agggggtaat gcaggtactc aactagcatc tactttagca
     5461 acttatagtt actacggata tattgaaaca gtctcaggaa atgctattgc cagtcaagca
     5521 tcatccattc taggaagtat tggtaggacg attggtagtt ttatcggatc aattagttta
     5581 atcctttcta atattgctga atccataaat gaactccttg gaaaattttt catgagtatg
     5641 aatatatttt ctcttatggg gtggggagaa ggtggatcgg atgtatctaa tccggtatct
     5701 aaagcattaa ctagcttgct taacaatttg ggattaaata atgaattatt taaagcgata
     5761 gcaagcttat ctatgctctt tttcacagca atactaggtt atcaaggaat gaaattattg
     5821 tcgaaagatg gtgttaactc gaagggatta tcaccagtac ttgaaaaatg gattgttcgt
     5881 atttttgtat tgtttatatt cttaccagtg gcaggatcgc tattgggagt ttatatcaac
     5941 ggcatccagt cagcaaaaaa agatggagat ataccaacaa atgttgtttc tgactacatc
     6001 attaacacaa gactttgggc ttctactcaa aatcttgctc catcatctga tactggtggt
     6061 gatgtacctg acggaaagcc tacatccgaa atcaagttcg ttgatacaga ttatgaacca
     6121 tctaaaaaat ctgcaatgat aagagcgatc aatactagca gttatgccag acaaaataat
     6181 gatctagatt ctaattcagt cggtatggct ctattaaaag aatggtcatc gaatgatact
     6241 ttcaatatta atacttatgc cggtgatgtt aatgtcgccg aaaatgatgc ttatatggga
     6301 agagcgcctg ctgtttggac aggctataat ttaggggatg gaaaagaagg aagtgcaatt
     6361 aagaaaccaa ccaatttaaa tggatatatt tggtcagcaa atcaaaatgt gacagacgac
     6421 aatcgcaagc ctgatggaaa aggaacagcg cataactttg atgctactca accttggggc
     6481 gttgctaaca atgcaacttt tagcacacag tcggtagctt tattgctcca atcggcactt
     6541 gatacatcgg gagctaagtt ttatgcgtat aatatcgctc cttcaggaat tcaaggcagt
     6601 gctaagaata tgtcaacagt aaaaacagaa tggcgtacag catccttagt tggtcatgga
     6661 gctatgggta agtttggctc gtggctagcg atgaacgctg aatcaatcgt tttagcagtt
     6721 ctttacttag catgtgcttg ggcgatgttc aatgtgaata tcgtgaaagc atttaaacgt
     6781 gaatttattc ggatttggga agtagttcta acaggatcac ttgcatcagg agcaggtgtg
     6841 atggcaattt cattaggtgt tgttacaaca tctctgatcg gactacaatt acctatcttt
     6901 ctgattactt ttattcgtgc atttggtgat actgctgtgg gattcattcc taatacatta
     6961 gttggcgatg gcgtagcaaa tattgttcga tctgctatga ttttaggtgt aggatatctt
     7021 ttagtcttta aaaaaggcga aaaagtaaat aatttgatta atatgcttat tggcttacca
     7081 cttgaagtag cgtttgggtt tgatgaacgt gctaagttat tatttggtaa tagaaatgat
     7141 gctgtgcgag ttgtggacgg cgtttctaaa gcaatcaaga aaagccaagg agctaaaaat
     7201 ccctattatg atggtggcaa cagctcagta aaagatatgg ttgcaggagg tcttggagca
     7261 gttggagcgc aaaaaatggc tgaacgtttg aacaatatgg gagatagcaa tgctgatgct
     7321 aagtccattg ctgataaagt aattcctgaa acaccagaag gaatgtcatt caacggtggc
     7381 ggagttggtt ccacaatcaa taatacctct ttcaaagatt tagttcgtca agatcaacta
     7441 cgtaataact cccaacaata tattgatcat ttaggacgta tggttgatga aaacggaaac
     7501 tttgtcgatg aaaatggtga aatattaaaa gatagtaaat acggtaaaga tgctaaaggc
     7561 aactttattg acgaaaaagg gcgtttggtc gatgaagatg gaaaccttat agatactaat
     7621 ggtaaaccat tggaagatag tgaagatggt aatattggta tggatagcga aggacgttta
     7681 attgatgaaa aaggtcgctt agtcgatgaa gatggaaatc ttgttgacga aaatggacgc
     7741 aggttgctaa atggtgattt tggtcatgat agtaacggac gtttagttga cgaaaaaggt
     7801 cgtttagttg atgaagatgg aaatcttgtt gatgagtttg gtaataaact tggtaaacac
     7861 agcgatggat ataatgtgaa tggagattta gtcgatgagg atgggaatat cattgagacg
     7921 agagatgcgc ttgtggcaga tcgaaataga gaactcgaac aacaacggca tacgccaatt
     7981 gctgttgcaa ttcctgagaa tgaagaagac gagattactt tagatcaaac tcatcaagaa
     8041 ctgaggaatg aaatgagtgg tgaggacgtg tcagacagag tgcatcggga attaggagat
     8101 ggagcatctg gtgaaagtca gtcaagagag cttaacagca actttgataa tagtgctatt
     8161 cggaatgaca cagtacttaa agacagagaa tcgctaacaa aagaaacgga taaagaacaa
     8221 tcagatgtga agaaaaagtt cattcctact cctaatgtta atcaaacaga taaatatgaa
     8281 acaaatattg gatcaggaat gtcgaaagac aatcaatcaa ttagagaacc tgaactgaca
     8341 aataaaaata atagaacaaa taattctaaa tctacttcag ataattcagg gaaatctaat
     8401 caatcaacca agaaaacatc aacagctaaa tcagccgttg aatatctatc aaaaactggt
     8461 aaaaatgttt acacctcagc agataaagct ttagcaaaag ctccaaaagg tagtctagca
     8521 gggataacct atgaagctaa aaacgaagct attaaaatag gtaaaggaac ggctaaggtt
     8581 gccaaggcta ctgctacagg agcatccaaa actggtaaag tagtgaagaa atctaccatg
     8641 tcagtcaagg actatattgt aaaatacggt aatcaaaaat caactgaaaa taagtctgaa
     8701 gacgagtaaa aagaaggagc attgatttgc tccttctttt tactttgatt tctaactgaa
     8761 gatgtcaagg aaaatttcta tttatgcaaa aaaattattt taaattgtct ataaatgaat
     8821 taaatattta ctgttcaaag ttgtataatt aaaaagggct tatgctggta aagaacagaa
     8881 ttattatcta aacaaaaact tgataaacca gcatttttag ccaataaaaa aagtgaaata
     8941 atttaatgga aaatctcttt taatcatcga aaatttatgt tattatgata acataaagat
     9001 gtaaatatcg agacgtcttt ttcttagtta gatcattgac aagtgaacaa ataagatttg
     9061 tcgatgatat aattaactta ttaaaaatat taaaaaggag gtttttatat gttcgattct
     9121 attaaaaatt tggttagcgg aatgacgcct gatatgaaaa ctttagtaca agttgttttg
     9181 tatttcttgg cattcgtttt ttcgttgaaa ccaggattca ctgcaatgaa attttttggt
     9241 gatgcaaaat ggggagatgg attaaaatat gttggggcga ttattgcaat ctttattatt
     9301 cctgtaatta caaccgttat gctttacaca atgggtacta aaacaggtaa tgatttgaac
     9361 caaaaagcta atatcatatc agcacttatt ccagttgttg gtatctatct agtttcaaaa
     9421 tttccaaaaa atgaactgtt aggttaaaag tgaattgaac aaatttggat aatatgccga
     9481 tttgttcaat ttacttttta ccaaaaaact acgctccaag cgtagtttag aagtgaggga
     9541 agaaagtaaa tgtaagatgg tttttgatag agaaaaaatg ttagctcacg ccaacgaagt
     9601 attaatgagc agtctgaaag gtaccgaact ggcaaagata atgaatatga atgttaacca
     9661 gttctatgat tatcgaaatg ggagtaaaaa gattgaaaaa gccagacttg aaacgcttat
     9721 taaattcgaa aaagcatatg tatatatgct agataaacag aaaagaacta ttgactgaaa
     9781 aaaggagttt tacaataatg gcaaaattca aagatattac cgaatctatt aattatgtgg
     9841 cagaaacagt tcctgtagtt gcactctctt tgaagccaca attactcgaa gtgtacaaaa
     9901 aagaagaaaa agaagcgaag gcaaaaaaag ctaaagcaaa ggtatggaaa gaattcgatg
     9961 cgttgctgac caaagattta ccaaagaaaa attatatggt aattgaagat gtacaaaagc
    10021 tcaacagtga gggagttata gatgaggatg atgctattct cgattctgag aagtatgtcg
    10081 ttctattgaa gtcaagtttg aattatgtag ctgatctacc agaaaaattt aaagagaagc
    10141 tattagcagg tgtccgtttt atggatagta gtggtcaatt attagttatt ccggatgatc
    10201 ctaatgtgtt tgaatcagaa gttgaagaag ctacagactt ctatagcgca gtagagcaat
    10261 tagaagtagc tactaatgga ttagaacgaa ttgctgagac cttcaactct tctgaagaag
    10321 ttgaatttga agccgtagca gaaaatgtac cagatgaaaa agaagctctt tttgtggatg
    10381 ataacaaacc aaccgaagca gaaaaccaag aagtaagcat agaagaaaca caaatacaaa
    10441 cagaaaccta ccaacaacca aaaattgatg aaaaacccga atcccaaagt gacaaagaaa
    10501 attatgttta tgaagctgaa aaaatcacat ctgaaagtga tgtaccgaaa aacttagatg
    10561 ctgatttttt agaaaaaaat tttgaacaga tcattgctga caaatttcaa cctgtagttt
    10621 tagatgagat taatgaccct gtcgatttaa acgaggagat gctatccata cctgagttga
    10681 aaccattgct gtttttagct aaagagagta tgaacgctcg catctctatt gctaataata
    10741 aaatcaacag tgaattaaat aagacgaagg agcaattgat cctcaaagga aagcaaatgt
    10801 tagctgatga gcttttgcgg atagaggaag caacctcatt aaatggtcaa aatcagttta
    10861 caaatacact atcggttgct aaagagagct acgaagcaga agcatctaaa attgatgatt
    10921 tagtgatgga aaaatccaat atcattcgta aagattatga tatggcgaaa caggcttatt
    10981 tggatgctga actaaagaaa ctagagcaac ggtatgatgc tgagcattta ccagaagtta
    11041 aacaacaaga agatgaatat agaaaagcaa ctgaagaaag tcttgatcaa cagtaccaag
    11101 aagcaattga atctattaaa aacgatgcat tgaaacaaag ccaagaagca aaagaagttg
    11161 tggtggaacg aattatcaat tcattgaaac cagaaattga aaaatcatta acagacgtgc
    11221 aagaaaaaac tttagagatt gtcgaaaaaa caaagtcaga aaatgaagct gagttccatg
    11281 atacaaaaga gaaaacaaca gatgtggttg tcaaatattt tgcgaacaaa gaagaagttg
    11341 aaaagcaggt agctaacaac acgaaagaat tagaaacaaa acttgtagag caacaaaaaa
    11401 ctatcgaaga atataaagat cgaatgaaag aggtcaaaga tttagcagag ggttataaat
    11461 taaatagcga aaagttggca gaacaaagtg aagctttaag agaagactta tctcgttccc
    11521 gaagtgatct agctcaaatg gcagaaaaaa cgaaatcatt agcagttgct acagaaaatc
    11581 aatcaagttc aaaacgtgat tttacaagta aaaagatggc tactatcatt tctgtcgctg
    11641 caatggcttg cggaacgatc ttgggatcgt cctactttat tagtcataat aatcaacaag
    11701 cacaggctca aactcaaaca tctatttcga gagtggaaaa ttcgattggg acaaatacga
    11761 actctgcttc aacaaatagt agctatgaag caacaccaac tacagagttc aaagttggga
    11821 aagatgttgt catcagtatt gatgggaaaa caactaaggc tgaagtgatt tctattgatg
    11881 gagaaaaagc agttgtgaaa actccagacg gaaatcagta caaaattcaa gctcacttat
    11941 ctgataataa ctaaggaggg ctattatgcg agaagaaaaa atagtttatc cagtaattca
    12001 aaacaatcaa gaaaacatga cctcttctca gcacttacag gaagcacagc tcgtgtatca
    12061 ggtaaatgaa ctaccacaaa cgaacgagac aatgagcaag tcaatatctt taattggctt
    12121 gcttattgtt gcaatagtga taactgtagg tgtttaccgt aagttgaacg tgaaaggaag
    12181 tatttctgat ggcagataac tcaaactatg gatttaaaca taacttacat caaaaacctt
    12241 ctgaaacaat tgtcagagtt gaatggcttg aaaagcatgt caatagcacc tattctgtta
    12301 cagcaaaaag attaattgta tcagctatag ttgttgcagt cgttgttctt ggatcaattg
    12361 tttacttagt ttctgatacg attaaactga aatttatttt tgttccaacg ttattaatga
    12421 taattggatt tcttattcta attagccagt atatgtttat tgatccatct agtggttcat
    12481 tgatttctgg ttctattaga ctgttcaaat cagtagttaa ttcagcaaga gtatctatgg
    12541 ggaaaaagaa accatttcga ggcacagaga ttttaaaagt gaaacaagat ggaacaatcg
    12601 tatatcgaga tgggagtgta gcattaatgt tcaatctaga tggggctaca tcactaaccg
    12661 cttttccaga agaagttatg gctcaggaat cgaaatcaat acaatatcaa aataccagag
    12721 atcgttcctt agctgaaatc aaaatcactt catctcaacc tcagaatacg gaaagacaaa
    12781 ctcgaaatat gaaaatacaa aaacgaaatg caaggagtaa caaagcagtc tttgacataa
    12841 tagatcagca agaacgattt gtatcagaat atattgatgg tgtgaaaaca acagtaatcc
    12901 aatatcttat cttgaaatct caatctaaga tggtattgga tgaaggtgtg gaacgtttag
    12961 aaaactatac gagtagagga ctttattata gtgtcactca gttgtcagct aaacaaacaa
    13021 aagaaatatt atctgatatt tatgcactga aatagaggtg agagattaaa tggaaattat
    13081 gggtagatca caactattaa atttcaaaaa aaagagaaaa actaaaaaag aacaaaaaac
    13141 tagaaattca aaggtagtga ataatacctt tgttagtgat ttaacatctt tagttggtta
    13201 tcaatttaca ccatcttaca ttaggacagg taagcggtgg ggaacaattg tgaaattagt
    13261 gaatacttat ggaatgaata gaaattatgg atttggttgg atggttaaag ctattcctga
    13321 agtgagagta gaaggggtaa aagcctatct aattgaagca gataagccag tgtcacaaaa
    13381 agatcaacaa gaaataatgc acgagaatgt tgggggatca acgaatagtt atgaaaataa
    13441 tttagaagga aaaaattcag atgctgaaat tagcaaaact gttatgcgga ttatggattt
    13501 gaatacagca tctttactag acagcatggc aaataaaatt attgattgga agctagaaat
    13561 cctattagta tccgattctc ctaataaaat cagcgaacag ctaattaaac tccaacggtt
    13621 gtatgatgaa aaaatgtatg gattaaagct tatgtcatat ggaggagatc aacgtgatct
    13681 gtttgataga cttttaagtg aaccaatcgg gaatgtctat gagtatacgt ctatgagttc
    13741 ggattttgca ggaaatgatc atgcaattcg caaaggttta gatgatgcga atggatgggc
    13801 tatcggcact atgtctacta gctacgcaag tggtgtagct tttatggcac tggatgattc
    13861 ttttacaagt aaagttttag tggcatcaca caaaaacgct acaattaaag gctatgatga
    13921 aaaaatctca gcatcatcac tttgggggca attgattgct aataatgcaa tggcaaatca
    13981 taaacgaact tttcatattg tcttaaataa atttgattac tttggtgatc caactggtag
    14041 aacatttgtt gccaaacctt cgttaaacag tgtaatagaa aaggttaatt tggctgaaga
    14101 aggtcttaac tctttggagc tttttggtaa tcaaaaggac attggtgatt tatatagacg
    14161 gaatctcgat aaactaactc agatttttta tttgttagca ggcagaacat tagattctgc
    14221 aactagagtt ttattgaaaa aagaattagg taacttttat ataagtcaga aactttggga
    14281 tattaattat gaagaacgtt caaaactttt ggtgaacagt agtgatacaa tgccagttct
    14341 tggggatttt attagctatt tgactaatgc tgaaacaaaa gccaaaaaat ctggtactga
    14401 aaaagaagtc gatcgtatta ttgtattaaa agacacattg agaacagcat tagacagctt
    14461 ctctaggatg tttaatcaaa gaacgacact gaaagaaatc ggtaaaagtg acaaattaca
    14521 gttttactat aacttaggtg atctaagagc agaaaccaat gttatggaag ctcaattttt
    14581 aaataccttt gatttcatca catcacaagc aaaaaaagat gatattgtta tgattcacgg
    14641 cttcgataag ttatctattg aaaccgttgg gctaattaaa aaaagattaa caatgcttga
    14701 agaagatgga attcgtcttg cctttctatt tgatacaatc ggttcaggag aagacaatga
    14761 atacaaagaa gatattgaaa cagctgatat attcaataca gatggaaaaa tttaccaaag
    14821 ttttgaaagt gattttaatt acactatcat tggaaagatg accagtggag agttaatgaa
    14881 gtatgaaaaa aaagttgctc ctgctagaaa aggagaagat gatcaaaaac tttctgttga
    14941 tttgagacag tatttgacaa caggaagaag taataaattt cagatcagga gaccagtgga
    15001 tctgactaat aattttgtta ttggagagtt tattctttaa gttttatctt tgattttgag
    15061 tgtataagga aaggataaga aaaaacatgt atatttgaga ctaaaaacag tcttagagaa
    15121 catggtacgt tttgtgaaaa gagaactata gaaatttcat aaagtgaaac cttactgagt
    15181 gaaataatta tagtggaggt taaataatga aaccaaacat aaaaatttta gatcgaatat
    15241 ttctaggtag agacacagaa gtaatcctaa ttcaacatga agaaggattt gaagttagta
    15301 taggtattca gaaacttcaa aaacctcatt attgtaatca gttgtataaa aactttactg
    15361 atgaagaaaa agcgagagtt t
//
LOCUS       DAZKCC010000068        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000068 DAZKCC010000000
VERSION     DAZKCC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.069"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG8VH_002175"
     CDS             2..1021
                     /locus_tag="ACG8VH_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9110720.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG8VH_002176"
     CDS             1018..1785
                     /locus_tag="ACG8VH_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9110721.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8VH_002177"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8VH_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP9110722.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG8VH_002178"
     CDS             3750..4070
                     /locus_tag="ACG8VH_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP9110723.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG8VH_002179"
     CDS             4070..4420
                     /locus_tag="ACG8VH_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9110724.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8VH_002180"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8VH_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP9110725.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8VH_002181"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8VH_002181"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9110726.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG8VH_002182"
     CDS             6414..7439
                     /locus_tag="ACG8VH_002182"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9110727.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG8VH_002183"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG8VH_002183"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9110728.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG8VH_002184"
     CDS             8517..8957
                     /locus_tag="ACG8VH_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP9110729.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG8VH_002185"
     CDS             8960..9562
                     /locus_tag="ACG8VH_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9110730.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG8VH_002186"
     CDS             complement(9625..10728)
                     /locus_tag="ACG8VH_002186"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP9110731.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8VH_002187"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8VH_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP9110732.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG8VH_002188"
     CDS             12294..12689
                     /locus_tag="ACG8VH_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110733.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG8VH_002189"
     CDS             12689..12940
                     /locus_tag="ACG8VH_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110734.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG8VH_002190"
     CDS             12983..13378
                     /locus_tag="ACG8VH_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110735.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG8VH_002191"
     CDS             13375..14631
                     /locus_tag="ACG8VH_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFP9110736.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG8VH_002192"
     CDS             14643..14966
                     /locus_tag="ACG8VH_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110737.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG8VH_002193"
     CDS             14959..15225
                     /locus_tag="ACG8VH_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110738.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKCC010000069        15231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000069 DAZKCC010000000
VERSION     DAZKCC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.070"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8VH_002194"
     CDS             278..787
                     /locus_tag="ACG8VH_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9110739.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8VH_002195"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8VH_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9110740.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8VH_002196"
     CDS             2322..3302
                     /locus_tag="ACG8VH_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9110741.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8VH_002197"
     CDS             3424..4842
                     /locus_tag="ACG8VH_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9110742.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8VH_002198"
     CDS             4913..6382
                     /locus_tag="ACG8VH_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9110743.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8VH_002199"
     CDS             6392..6859
                     /locus_tag="ACG8VH_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9110744.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8VH_002200"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8VH_002200"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9110745.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACG8VH_002201"
     CDS             7849..10383
                     /locus_tag="ACG8VH_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9110746.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACG8VH_002202"
     CDS             10539..11312
                     /locus_tag="ACG8VH_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9110747.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACG8VH_002203"
     CDS             11305..12096
                     /locus_tag="ACG8VH_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9110748.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACG8VH_002204"
     CDS             12106..12597
                     /locus_tag="ACG8VH_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9110749.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACG8VH_002205"
     CDS             12615..13025
                     /locus_tag="ACG8VH_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9110750.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACG8VH_002206"
     CDS             13029..14429
                     /locus_tag="ACG8VH_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFP9110751.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACG8VH_002207"
     CDS             14452..14826
                     /locus_tag="ACG8VH_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9110752.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2137 c         3045 g         4762 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta g
//
LOCUS       DAZKCC010000070        14994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000070 DAZKCC010000000
VERSION     DAZKCC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.072"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8VH_002208"
     CDS             <1..608
                     /locus_tag="ACG8VH_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110753.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACG8VH_002209"
     CDS             628..1656
                     /locus_tag="ACG8VH_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9110754.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACG8VH_002210"
     CDS             1653..2411
                     /locus_tag="ACG8VH_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9110755.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACG8VH_002211"
     CDS             complement(2497..3237)
                     /locus_tag="ACG8VH_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9110756.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACG8VH_002212"
     CDS             3298..4551
                     /locus_tag="ACG8VH_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9110757.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACG8VH_002213"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACG8VH_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACG8VH_002214"
     CDS             5222..5548
                     /locus_tag="ACG8VH_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9110758.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACG8VH_002215"
     CDS             5616..6329
                     /locus_tag="ACG8VH_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9110759.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /gene="licT"
                     /locus_tag="ACG8VH_002216"
                     /pseudo
     CDS             6639..7491
                     /gene="licT"
                     /locus_tag="ACG8VH_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7612..9477
                     /locus_tag="ACG8VH_002217"
     CDS             7612..9477
                     /locus_tag="ACG8VH_002217"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9110760.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9491..10934
                     /locus_tag="ACG8VH_002218"
                     /pseudo
     CDS             9491..10934
                     /locus_tag="ACG8VH_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11042..11743
                     /locus_tag="ACG8VH_002219"
     CDS             11042..11743
                     /locus_tag="ACG8VH_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9110761.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11869..12381
                     /locus_tag="ACG8VH_002220"
                     /pseudo
     CDS             11869..12381
                     /locus_tag="ACG8VH_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12779..12988)
                     /locus_tag="ACG8VH_002221"
     CDS             complement(12779..12988)
                     /locus_tag="ACG8VH_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFP9110762.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13229..13804
                     /locus_tag="ACG8VH_002222"
     CDS             13229..13804
                     /locus_tag="ACG8VH_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP9110763.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="ACG8VH_002223"
     CDS             13804..14244
                     /locus_tag="ACG8VH_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9110764.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="ACG8VH_002224"
     CDS             14260..14961
                     /locus_tag="ACG8VH_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9110765.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2485 c         3161 g         4282 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DAZKCC010000071        14700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000071 DAZKCC010000000
VERSION     DAZKCC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.073"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..833)
                     /locus_tag="ACG8VH_002225"
     CDS             complement(267..833)
                     /locus_tag="ACG8VH_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9110766.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(833..1789)
                     /locus_tag="ACG8VH_002226"
     CDS             complement(833..1789)
                     /locus_tag="ACG8VH_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9110767.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1789..2445)
                     /locus_tag="ACG8VH_002227"
     CDS             complement(1789..2445)
                     /locus_tag="ACG8VH_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9110768.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2815..4833
                     /locus_tag="ACG8VH_002228"
     CDS             2815..4833
                     /locus_tag="ACG8VH_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9110769.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4903..5109
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5260..7674
                     /gene="leuS"
                     /locus_tag="ACG8VH_002229"
     CDS             5260..7674
                     /gene="leuS"
                     /locus_tag="ACG8VH_002229"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9110770.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8022..9575
                     /locus_tag="ACG8VH_002230"
     CDS             8022..9575
                     /locus_tag="ACG8VH_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP9110771.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9956..11740)
                     /locus_tag="ACG8VH_002231"
     CDS             complement(9956..11740)
                     /locus_tag="ACG8VH_002231"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110772.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11755..13476)
                     /locus_tag="ACG8VH_002232"
     CDS             complement(11755..13476)
                     /locus_tag="ACG8VH_002232"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110773.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13576..14337
                     /locus_tag="ACG8VH_002233"
     CDS             13576..14337
                     /locus_tag="ACG8VH_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9110774.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4774 a         2710 c         2925 g         4291 t
ORIGIN      
        1 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
       61 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      121 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      181 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      241 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      301 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      361 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      421 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      481 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      541 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
      601 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
      661 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
      721 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
      781 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
      841 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
      901 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
      961 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1021 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1081 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1141 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1201 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1261 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1321 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1381 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1441 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1501 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1561 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     1621 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     1681 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     1741 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     1801 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     1861 cagccaagaa agactgagta agtaaccgcc gatgatatca gtaggaaaat gaacgcccag
     1921 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     1981 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2041 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2101 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2161 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2221 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2281 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2341 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2401 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2461 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2521 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     2581 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     2641 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     2701 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     2761 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     2821 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     2881 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     2941 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3001 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3061 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3121 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3181 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3241 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3301 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3361 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3421 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3481 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3541 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     3601 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     3661 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     3721 gaaaatggct cttcgacgaa taattccggt aacaatacca attcttcagg aaatgctggt
     3781 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     3841 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     3901 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     3961 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4021 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4081 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4141 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4201 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4261 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4321 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4381 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4441 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4501 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4561 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     4621 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     4681 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     4741 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     4801 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     4861 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     4921 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     4981 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5041 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5101 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5161 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5221 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5281 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5341 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5401 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5461 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5521 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     5581 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     5641 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     5701 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     5761 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     5821 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     5881 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     5941 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6001 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6061 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6121 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6181 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6241 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6301 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6361 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6421 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6481 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6541 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     6601 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     6661 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     6721 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     6781 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     6841 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     6901 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     6961 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7021 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7081 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7141 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7201 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7261 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7321 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7381 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7441 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7501 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7561 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     7621 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     7681 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     7741 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     7801 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     7861 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     7921 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     7981 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8041 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8101 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8161 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8221 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8281 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8341 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8401 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8461 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8521 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     8581 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     8641 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     8701 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     8761 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     8821 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     8881 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     8941 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9001 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9061 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9121 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9181 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9241 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9301 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9361 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9421 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9481 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9541 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
     9601 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
     9661 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
     9721 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
     9781 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
     9841 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
     9901 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
     9961 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10021 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10081 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10141 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10201 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10261 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10321 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10381 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10441 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10501 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10561 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    10621 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    10681 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    10741 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    10801 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    10861 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    10921 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    10981 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11041 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11101 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11161 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11221 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11281 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11341 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11401 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11461 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11521 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    11581 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    11641 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    11701 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    11761 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    11821 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    11881 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    11941 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12001 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12061 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12121 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12181 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12241 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12301 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12361 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12421 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12481 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12541 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    12601 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    12661 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    12721 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    12781 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    12841 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    12901 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    12961 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13021 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13081 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13141 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13201 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13261 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13321 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13381 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13441 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13501 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13561 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    13621 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    13681 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    13741 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    13801 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    13861 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    13921 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    13981 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14041 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14101 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14161 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14221 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14281 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14341 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14401 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14461 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14521 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    14581 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    14641 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
//
LOCUS       DAZKCC010000072        14549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000072 DAZKCC010000000
VERSION     DAZKCC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.074"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8VH_002234"
     CDS             4..816
                     /locus_tag="ACG8VH_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP9110775.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8VH_002235"
     CDS             813..1613
                     /locus_tag="ACG8VH_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9110776.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8VH_002236"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8VH_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP9110777.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8VH_002237"
     CDS             3110..4819
                     /locus_tag="ACG8VH_002237"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9110778.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG8VH_002238"
     CDS             5031..9389
                     /locus_tag="ACG8VH_002238"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP9110779.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8VH_002239"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8VH_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9110780.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8VH_002240"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8VH_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9110781.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG8VH_002241"
     CDS             11227..11520
                     /locus_tag="ACG8VH_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP9110782.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG8VH_002242"
     CDS             11517..11828
                     /locus_tag="ACG8VH_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP9110783.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG8VH_002243"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG8VH_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9110784.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8VH_002244"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8VH_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9110785.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DAZKCC010000073        14449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000073 DAZKCC010000000
VERSION     DAZKCC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.075"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(263..3916)
                     /gene="rpoC"
                     /locus_tag="ACG8VH_002245"
     CDS             complement(263..3916)
                     /gene="rpoC"
                     /locus_tag="ACG8VH_002245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9110786.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3995..7612)
                     /gene="rpoB"
                     /locus_tag="ACG8VH_002246"
     CDS             complement(3995..7612)
                     /gene="rpoB"
                     /locus_tag="ACG8VH_002246"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9110787.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8340..9308
                     /locus_tag="ACG8VH_002247"
     CDS             8340..9308
                     /locus_tag="ACG8VH_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9110788.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9326..10231
                     /locus_tag="ACG8VH_002248"
     CDS             9326..10231
                     /locus_tag="ACG8VH_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9110789.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10228..11034
                     /locus_tag="ACG8VH_002249"
     CDS             10228..11034
                     /locus_tag="ACG8VH_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110790.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11191..12159
                     /locus_tag="ACG8VH_002250"
     CDS             11191..12159
                     /locus_tag="ACG8VH_002250"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP9110791.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12476..13000)
                     /locus_tag="ACG8VH_002251"
     CDS             complement(12476..13000)
                     /locus_tag="ACG8VH_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP9110792.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13287..>14449
                     /gene="scm"
                     /locus_tag="ACG8VH_002252"
     CDS             13287..>14449
                     /gene="scm"
                     /locus_tag="ACG8VH_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9110793.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTS"
BASE COUNT         4105 a         3114 c         2835 g         4395 t
ORIGIN      
        1 ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat
       61 ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga
      121 tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt
      181 tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt
      241 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
      301 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
      361 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
      421 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
      481 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
      541 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
      601 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
      661 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
      721 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
      781 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
      841 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
      901 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
      961 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
     1021 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
     1081 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
     1141 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
     1201 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
     1261 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
     1321 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
     1381 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
     1441 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
     1501 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
     1561 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
     1621 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
     1681 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
     1741 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
     1801 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
     1861 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
     1921 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
     1981 agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt
     2041 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
     2101 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
     2161 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
     2221 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
     2281 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
     2341 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
     2401 cttccattgt caagtagtag ttacctagaa ccatatcttg ggaaggagta acaactggtt
     2461 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
     2521 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
     2581 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
     2641 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
     2701 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
     2761 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
     2821 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
     2881 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
     2941 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
     3001 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
     3061 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
     3121 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
     3181 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
     3241 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
     3301 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
     3361 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
     3421 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
     3481 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
     3541 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
     3601 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
     3661 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
     3721 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
     3781 acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
     3841 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
     3901 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
     3961 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
     4021 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
     4081 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
     4141 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
     4201 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
     4261 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
     4321 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
     4381 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
     4441 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
     4501 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
     4561 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
     4621 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
     4681 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
     4741 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
     4801 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
     4861 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
     4921 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
     4981 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
     5041 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
     5101 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
     5161 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
     5221 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
     5281 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
     5341 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
     5401 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
     5461 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
     5521 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca
     5581 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
     5641 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
     5701 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
     5761 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
     5821 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
     5881 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
     5941 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
     6001 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
     6061 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
     6121 atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg
     6181 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
     6241 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
     6301 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
     6361 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
     6421 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
     6481 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
     6541 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
     6601 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
     6661 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
     6721 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
     6781 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
     6841 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
     6901 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
     6961 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
     7021 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
     7081 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
     7141 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
     7201 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
     7261 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
     7321 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
     7381 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
     7441 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
     7501 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
     7561 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
     7621 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
     7681 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
     7741 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
     7801 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
     7861 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
     7921 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
     7981 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
     8041 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
     8101 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
     8161 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
     8221 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
     8281 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
     8341 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
     8401 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
     8461 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
     8521 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
     8581 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
     8641 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
     8701 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
     8761 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
     8821 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
     8881 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
     8941 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
     9001 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
     9061 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
     9121 tagcaacagt aggaatcgac tacgaaagct tgggtaaaca aacagggatc atggcagcaa
     9181 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
     9241 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
     9301 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
     9361 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
     9421 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
     9481 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc
     9541 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
     9601 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
     9661 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
     9721 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
     9781 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
     9841 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
     9901 tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa
     9961 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
    10021 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
    10081 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
    10141 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
    10201 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    10261 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    10321 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    10381 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    10441 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    10501 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    10561 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    10621 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    10681 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    10741 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    10801 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    10861 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    10921 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    10981 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    11041 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    11101 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    11161 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    11221 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    11281 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    11341 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    11401 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg
    11461 atgaaagatg tcaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    11521 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    11581 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    11641 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    11701 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    11761 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    11821 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    11881 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    11941 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    12001 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    12061 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    12121 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    12181 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    12241 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    12301 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    12361 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    12421 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    12481 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    12541 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    12601 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    12661 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    12721 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    12781 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    12841 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    12901 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    12961 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    13021 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    13081 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    13141 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    13201 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    13261 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    13321 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    13381 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    13441 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    13501 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    13561 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    13621 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    13681 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    13741 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    13801 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    13861 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    13921 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    13981 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    14041 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    14101 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    14161 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    14221 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    14281 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    14341 cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt gatagcacgg
    14401 atacatcagc ttcaagcgac agtacagata ctacctctac cacaagtga
//
LOCUS       DAZKCC010000074        14217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000074 DAZKCC010000000
VERSION     DAZKCC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.076"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(198..2195)
                     /gene="tkt"
                     /locus_tag="ACG8VH_002253"
     CDS             complement(198..2195)
                     /gene="tkt"
                     /locus_tag="ACG8VH_002253"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9110794.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2297..2548)
                     /locus_tag="ACG8VH_002254"
     CDS             complement(2297..2548)
                     /locus_tag="ACG8VH_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP9110795.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2695..3318
                     /gene="lexA"
                     /locus_tag="ACG8VH_002255"
     CDS             2695..3318
                     /gene="lexA"
                     /locus_tag="ACG8VH_002255"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9110796.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3498..3716
                     /locus_tag="ACG8VH_002256"
     CDS             3498..3716
                     /locus_tag="ACG8VH_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110797.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4005..4517)
                     /locus_tag="ACG8VH_002257"
     CDS             complement(4005..4517)
                     /locus_tag="ACG8VH_002257"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9110798.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4531..6828)
                     /gene="recJ"
                     /locus_tag="ACG8VH_002258"
     CDS             complement(4531..6828)
                     /gene="recJ"
                     /locus_tag="ACG8VH_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9110799.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6970..7413)
                     /locus_tag="ACG8VH_002259"
     CDS             complement(6970..7413)
                     /locus_tag="ACG8VH_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP9110800.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7414..8202)
                     /locus_tag="ACG8VH_002260"
     CDS             complement(7414..8202)
                     /locus_tag="ACG8VH_002260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9110801.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8199..9140)
                     /gene="rnz"
                     /locus_tag="ACG8VH_002261"
     CDS             complement(8199..9140)
                     /gene="rnz"
                     /locus_tag="ACG8VH_002261"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP9110802.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9317..9904)
                     /locus_tag="ACG8VH_002262"
     CDS             complement(9317..9904)
                     /locus_tag="ACG8VH_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP9110803.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10125..11438)
                     /gene="obgE"
                     /locus_tag="ACG8VH_002263"
     CDS             complement(10125..11438)
                     /gene="obgE"
                     /locus_tag="ACG8VH_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP9110804.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11726..12736
                     /gene="gap"
                     /locus_tag="ACG8VH_002264"
     CDS             11726..12736
                     /gene="gap"
                     /locus_tag="ACG8VH_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9110805.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12821..13756)
                     /locus_tag="ACG8VH_002265"
     CDS             complement(12821..13756)
                     /locus_tag="ACG8VH_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9110806.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13942..>14217
                     /locus_tag="ACG8VH_002266"
     CDS             13942..>14217
                     /locus_tag="ACG8VH_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110807.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT         3969 a         3161 c         2507 g         4580 t
ORIGIN      
        1 gaaagttttg tctcgcttaa tttgacaaat aatattctca agaaacacaa actaatccga
       61 agaattcttt gttttgtcat ttaaatgtaa catagccgaa aacgaaaagt aatattgatt
      121 tattcttcta acacataaag aacagctgtc tccaacttgc agacagctgt tcttatcagt
      181 caaacaattg cttggatcta tgatagttga ttgtacgtat tcacgacatt ttctactgta
      241 aatccaaatt cttctaaaat tttagtgcca ggggcagatg caccgaaatg atcgattgta
      301 atcgttgcgc cctctgttcc tacgtagcgt tcccatccaa atgatgcagc agcttcgata
      361 gccacacgtt tcttcactga tttaggaagt acactttctt tatattcaga agattgtttt
      421 tcaaagaggt caaagcttgg catagaaaca acagaaacat ctttgccttt ttcagccaat
      481 tctttttgag ctttcacagc caaatcaact tcagaacctg ttgcaatcaa aataccttct
      541 ggtgtttctc cttgtgatgg agaaagaaca tacgcgcctt ttttcaccat ttcatctgct
      601 acttcttttg tagaaggcaa tactggaagg ttttgacgac tcaatacaag gatagtaggc
      661 gcatctgttg tctccatcgc tactttccaa gcagcacgtg tttcatttcc gtctgccgga
      721 cgaatcactt gaacaccagg catgcagcga acagatgcta gttgttcaat cggttcgtgc
      781 gttggtccgt cttctccgac agcaaccgag tcatgtgtta agacgtaaac aacaggagta
      841 tgttggattg ctgctaaacg aactgctggt cttagataat cgacaaagac aaagaatgtt
      901 ccaccgtaga tacgagttcc gccatgaagt tggataccat tcatagccga agccattgca
      961 aattcgcgga caccgaacca gatgttgcgt ccttcataat gttcaggagt gaaatcttta
     1021 tctgcagcca ccattgtatt attggaacct gacaagtccg ctgaaccacc ccagaagctt
     1081 ggtatagctt tcgataattc ttggatgact tctttacttg aaacacggct tgcttgactg
     1141 cttccaactt cataagttgg caattctgca tcccagtttt ctggtagttt gccatcaaaa
     1201 gcatcttcaa attgttgtgc taattcagga taagcttttt tataattggc aaacatttca
     1261 ttccatgcat cttcagcttt ttgtccttct tctatcatcg tttgatggaa gcgggcagct
     1321 acttcttctg gaacagtgaa atctggatac tcccaaccat aaacttcttt agccatcttg
     1381 atgccatctt caccaagagg agcaccatga acagcagatg ttccttcttt aggagcacca
     1441 tagccgatga ctgtttttac ttcgatcaat gttggtttgt ctgtttcagc ttttgcttct
     1501 tcaatcgctt tagagattgc ttctaggtca tttccgtctt tcactaagat atgttgccag
     1561 ccatatgctt catagcgagc accaacgttt tctgtgaaag cttttgaagt tggaccgtct
     1621 agtgaaatat cgtttgaatc gtataaaacg atcaatttcc ctagtttcat atgacctgcc
     1681 atcgaacttg cttcttgaga taccccttcc atcaagtctc cgtcaccgca tattgcatac
     1741 gtatagtgat ccatcacatt aaagttttct ttgttatacg ttgctgcaag gtgcgcttct
     1801 gccattgcca taccgacagc cattgcaatc ccttgtccta atggccctgt agtggcttct
     1861 acaccatctg tatgatgtac ttcaggatgt cctggtgttt tagaatccca ttgacggaat
     1921 tgtttcaaat catcgattgt cacttgatag ccagctaaat gaagcaagct atacaacatt
     1981 gctgacccat gtcctgccga aagaacgaaa cggtcgcggt cagcccagtt ttttgatgtc
     2041 gttggattta ctttcaggtg ttttgtccat aacgcatatg ccataggtgc tgcgcccatc
     2101 ggcaatccag gatgtcctga gtttgctttt tggactgctt caatacttaa tgtacgaatt
     2161 gtgttaacgc caagttggtc aattttgtca aacaaaataa cccctcctat taatttaggt
     2221 tttttttacc tttatttctt acactttcat tctataagat gtcacacgaa aaagcaatct
     2281 ttttcagtat tttttactaa aagttgtcat ttcggttgtg taagcctttt ttcttttgga
     2341 tttcttttaa tttttccggg gtcacgtcat tgccctcagg atcgacgact ttcattcctt
     2401 cgatatgatg acgcataccg gaacgaaaag cttccaaata ctctttgcgc aagttgattt
     2461 gttcttgttc ttcggctttt gagagaccat tttccttctt tttacgtgca agttcattga
     2521 tccgctctat tttttcaggt gacagcatgc tcagccctcc ttttactctc cttcatatta
     2581 tataaaaact gaaaaaaaaa caactagata aactaaataa aatacgaaca ttcgtttgat
     2641 ttagcgcttt ttatatggta ctatttattt atcaagtgaa agaaggtgta ccttgtggcc
     2701 cgacaaactg aatcaagaca aatcgaagtc ttacgatata tccatgaaca agtaacccaa
     2761 aaaggatacc cgccgactgt ccgtgaaatc ggggaagctg ttcagctttc ttctacttct
     2821 actgttcacg gtcatcttgc acggcttgaa aaaaaagggt ttatccaaag agatccgacg
     2881 aaacctcgtg caatcgagtt gacaaaacaa ggtttagaaa agattgggat tcgtccaaca
     2941 actattccag tacttggcgt cgtaacagct ggagaaccca ttttagcagt agaggatgct
     3001 tcagacttct ttccaatccc tcctaatctt tcttccgaag aaggcagtct ctttatgctg
     3061 acaattcgtg gagatagtat gatcaatgct gggatattgg atggtgacaa cgtgatcgtt
     3121 cggaagcaag aaactgctca aaatggcgat attgtcattg caatgactgc cgaggacgaa
     3181 gcgacttgca aacgattcta taaagaaaaa aactttttcc gtctgcagcc tgaaaacgac
     3241 ttgctcgaac caatcatctt agatcaagta tctatattag gacgagtagt aggactttat
     3301 cgtagtcata tctattaatt gctactaatt aaaagaaaac tttttaaaaa gagtttgaga
     3361 cgttggtctc aaactctttt ttcgcccggg ttcttcttct ttcaaatttt tatcacttcc
     3421 ctctttacaa accgaacaaa tattcgtata ctaaatatac gaacaattgt tcgcataaat
     3481 taaagaggtg agaattcatg aaatcaatga gacgtcttgg cttagtatta actgttttgt
     3541 ttttaggtat tattattggt aatttcggca tacgtccagc tttacttgtg tttgttccgt
     3601 tatttacttt gtggttcatg ttgtgggatg aaaagaagta ccgtcaatct gagcggcgca
     3661 gaattcatga agatcatttc tatcatgagc cctactacag atccaggcaa tcataataag
     3721 gattgactgc taattattca aaagacaaga agttaaactt ttacgggggt gtgacaaaaa
     3781 tcggtcacat cctttttttc gaataaacgg tgttctaaag ccagtttttc ggtattaagt
     3841 caaattgcgg aaaaagtgat tttttaaact tctactggtc tttgcttgtt tccatttata
     3901 aaaaaatagc gaccctcaaa agtcagatag agaatctgac ttttgagggt cgctttaaaa
     3961 tcgcttcttt aagaataaca tcaagaatag cttctttttt ctgcttaata ttccattaat
     4021 gtaatgatgt catatccttt gattttgtcg cgtccatgga gatccatcaa ttcgatcaaa
     4081 aatgcgcagc ctacgactac accgcctaat tgttcgatca gttcgatcgt cgctttgatt
     4141 gttcctccag tagctagcaa atcgtcacaa atcagcacac gttgacctgg tttaatggca
     4201 tccttatgca atgtcaatgt atctgttcca tattctaatc cataagtcac ttcgatggtt
     4261 tctcgaggta acttcccttt tttgcggact ggggcaaaac cgactcctaa ctcataagca
     4321 acaggacatc ctacgatgaa tccacgggct tctggcccaa ctaccatatc aattcgtttt
     4381 tcttttgcat aatttacgat ttgtttagta gcttcccgat aagcgtcacc atctgccatc
     4441 agtggggaaa tatctcgaaa cacaattcct tttgacggat agtcgggaat actggcaata
     4501 taatctttta aatccactct ttgtttcctc ctaggttgtt aaccatttct ttatggtagg
     4561 aatatcgctc aaaagcaaaa attcctcaac tttgatttta ttgagccgtc gctggtataa
     4621 tctgctctca gtcaaaggat gactttctgg gctagcgact ttttgtaaaa caccgttctc
     4681 tattgtaaca aatttcaatt caaaaaacac ctgtatcata aaaattaata atttttcagg
     4741 gatacgcaaa tattgagcta ttgcttttat tttatgacgt atatcaattc gttcttgaga
     4801 atggatcaat ttaaacaatt ttgcgtactg ttctcttgtg cctaccccat tcaaataggc
     4861 ctcatcaggt gaaatcccca tcagataaac acgattgaat gaataagacc gaaaaacttc
     4921 tttcagatcc gctaaatcat ctggacaatc cataacaact agagagtttg gttttctttt
     4981 ttcaattatt gcctttacag aatctagtcc ttcgaaaaca atcgcagctt cctggataat
     5041 ctcaggcacg aatttcaacg tcgaagaatc aaatgctaaa taaaggctac cttccgacgt
     5101 tcggctctgt tcacggaaac gttttgcccg ccaatcaaat acttgcagac cacccacaga
     5161 aaaatcagat accatcagct gcggtttctt acgcccgttc cattcattga tcgaaagctt
     5221 tcctaccaca tctacctgat tattcagcag ttcttcttct tgagtaccaa aaccaaaagc
     5281 gaccgcatca agctgactcg atgcatcgga aaggacaaat ttcagatgct gttggtttgc
     5341 tccgattctt ttgatatttt ctgcttggac ttgtcgaaat aaaaactgag gcaaaggatt
     5401 atccgtacca aatggcgcca atagtttcag ctcatcaatt cgctctactg ttacttcatt
     5461 tggcagcaat acttcatcaa ttctcaactc aggccctctc atcaagtcaa tttgatgatc
     5521 gactatatat tggttcatct tctcctgtag tatagtgaca ttttcacttg gcatcgttaa
     5581 accgactgct gcatgatgtc ctccgaagta agtaaataaa tctcgcatct ggtcaagcat
     5641 ttcaaaaaga ttcaaggctt caatactacg tccagatcct ttagctgatc catcttcttt
     5701 taaagtaagg acaagcgttg gtttacccgt ttcattcatt attttcccag caacaatgcc
     5761 caaaacgcct tcatgccatc ctcggtttac caatagatgg atatgattct ccttattgac
     5821 catcgctaat gcttcttgtg ttatctgctc tacgattgct tttctttctt cattgatctc
     5881 attcaatttt tttgcttgaa cggatgcttc ctcttcatca aacgtcgcaa gtaacgagac
     5941 ggcaggacgt ggatcgccca tccgaccgat tgcatttagt ctgggagcaa tcgaaaaacc
     6001 gatcgttgtt tcgtctgcgt ccctcatttt tacgccgctt tcttcaaata gcgcctgaag
     6061 tcctatccgt tctgaatgat gaatggcatc gattcctaat gaaacaagga ttcgattctc
     6121 atcagtcaat gaaactaaat cggcgatcgt tcctatcgct actaaatcca aaaattctgc
     6181 aggcggttct tccaataatg cagaagctac tttgaatgct actccgactc ctgccaaatc
     6241 tggaaatgga tagtgccccg ctggatgacg agggtgaaca atcgcataag cgtctggtaa
     6301 attctcaggt agttcgtgat gatctgtaat gatcacgtct acaccagctt gctgagcgta
     6361 tgctaccgca tcatttcctg ctaccccgtt atcaacagtg atgatcaact ggattccttc
     6421 ttcgatcttt tcttggtata ccgattggtt tggtccatac ccatgctcaa atcgatttgg
     6481 caggaatact tctacttctg caccaagtaa ttccagtgtt tctttcatta ccgttgcact
     6541 ggtgatccca tctgcgtcat aatcaccgta aacgaggatt ttttctccac tgattacagc
     6601 ttcttggatt cggctgattg ctttatccat atcgtgtatc agatacggat catgtaattc
     6661 ttgttccttt ggatgtaaaa atcggttgat atccttttcc tcatggtaac ctctttgcca
     6721 cagtaactta ccgacaaatg gagataattt cagttcacgg atcatttgga gaaaggattc
     6781 agaaggctct gtctcgctta acttccaatc aaaatttgct tgtctcactt tctcactcct
     6841 aacttagtta ttatagcaaa ggaaatcagc aaaaaaaagc agaaagaaag aataaaaaca
     6901 gatcagatcc gctagacaga tgttcagcag gacctgatct tttgtacgac catttatgaa
     6961 atatccctat tagtcaaaat aattgaagct ttgtttgggc gattgagttt tttgttcttc
     7021 cggcgctttc aattgactga tttgtgcggt tttcgcttgt aactccgcct gataagcagc
     7081 ttccaactcc gcacgttcgt ttctaaactc gcgttgctgt tcttcttttg cttcttcaat
     7141 tttcttttga agttctgttt gatattctga catttcctga gtcagctgtt tgatttgctt
     7201 tttttgctgc catagtgttg ccgaagcagt caaaaataca atcagtgcac caatcagcgc
     7261 agaaccgatg atcactaaga tcaatggcgc agaaaaggaa gtgaagccaa aacttacagg
     7321 aacattttta ttgttcaata cagcaaaaag aacaataatc aaaactaaaa caaatccaag
     7381 aatcacattg ctttgctttt tcaattcctt cacctacttt ttgttaaaaa ttccgctggc
     7441 taaataatca ccaatcgttg gaaacagcgt ataaaatttt gatgcaactt ctaaaataaa
     7501 cggttgattg atttcccgtt ttggatgtct catattgcgg acgatcattt ttgccaactt
     7561 ggcaggttct aaaacgattt gtccgacttt ttccaaatag cttccgcttg gatcagcctt
     7621 atcgaagaaa tttgtctcga ttggacctgg attcactgtc gtaacagcta cgccaagtgg
     7681 tttcaattct aatcgcaaag cgttggagaa gccaaggact gcaaatttcg tggcagaata
     7741 aaccgttgat tttgcagttg ccatttttcc agccatagag gcgatattaa tgatatgacc
     7801 ttgtcttctt tctgccatat cgatcgcaaa tttttgtgtc aaaaccatca ttcctaagat
     7861 gttgacttca aacatatcgt atgcttttcc aaggtcgaat gtaaggaagt cttcgaaaat
     7921 cccaaaaccc gcattgttta ccagaatatc gacgcgcccg acttcttcca tcaccttttc
     7981 atataattcg tccacgcttt caggattgct gacgtctacc ttgaaagcat aagcctcttt
     8041 gccgctcaag cgtctgcact cgtctctgac accttctaca aatgccattc ttcgtgcgca
     8101 acttataacg attgctcctt gctttgctgc ttcataacag atttgtgcgc ccagaccgcc
     8161 tgaactacct gttactaaaa caactttatc ttctaaattc atgcctcttc ctcctcgcga
     8221 aatggaatct caatgctatc aaaatctttc ataatcgtcg tgttttcaaa cacttcttgt
     8281 gcttcttttt caagctctaa agctgctttt cctaaatatc tcgcactgat atgagtaagt
     8341 agcagccgtt ttgcctgcgc ttcttttgca acttcagcag cttgatgagt cgtggaatgg
     8401 aaatagttgt gcgccattct tgcttcatct ttattaaatg tgctttcatg taccagaata
     8461 tcgctatttt ccgcaagcac aacggaatta tgtgtttttc tcgtatcgcc taaaatagtc
     8521 acgattcgtc cttttttatc tggacctacg aaatcttttc cattgatcgt ccgcccatct
     8581 tctagttgga tcgtttcacc ttgcttcagt ttgccgtaca gtggtccagc tggaatacct
     8641 aattctttta gacgatccac ctgcaattct cctttatgat catgttcaac cacacgataa
     8701 ccaaaactgt cgatcccatg atttagtttt tttgtataaa cagaaaattg ctgatcagta
     8761 aaaatcgggg ctgtttcatt tagctcaatg aatttcagcg gataacttaa acgcgtttgc
     8821 gaaataccta aagagacttt tatgtattcc tctatccctt ttggcccgta aatctctaat
     8881 ggcgtatccc caccttgaaa tgaccggctg ctgatgagcc cgggaagtcc aaaaatatga
     8941 tcgccgtgta aatgtgtaat gaaaatttta ccgatttttc tcggacgaat agtcgttcgc
     9001 aaaatctgca tttgcgtgcc ttcgccacag tcgaacaacc aaacttcatt gcgctcgtct
     9061 aaaagtttca atgctatact tgtcacattt ctatgttttg caggaacacc tgcacctgtt
     9121 cctaaaaatt gtaattccat aatctacctg actttcttta agttcctctt taattttagg
     9181 gtaaaaagaa gggataatca accgtaagtc gagaatgggt tcaaattaaa aataacgttc
     9241 taaagcgata gcgttttctt tgaaaattac tataaaaaca gcagcgaacc cgtttgagat
     9301 cctctgctgt tttttattaa tttgaagatc cagaactttc attgtcgttg gaagtttgcg
     9361 gatgtttgtc cttccaagtc ttttgtgcag cttctttcgc atgattttca gcttctttcg
     9421 catcatgcac atctttttcg acatcttcat aatccaagcg agtaatttct ccgcctaatt
     9481 ccatcattac caattgattc agtgggcgat tgtcctcttc atcagcaatc aagatcagtc
     9541 ctgtagctcc ttcaccgatt ttatttccta caaagtcgaa cacggtctgg gcatctttta
     9601 tttctttggc atcttgtgtc gcaccgatca ttcctccggc aaaccatcct aataaaatcc
     9661 ctagcggacc acctaataca ccaaccaaca ttccgattaa tccacccgta gaagatttgt
     9721 tattaccggt aaagtcgata aaatcttcta ttttgaattg atgtgtccca ttaggatcat
     9781 ggctgacaac tgccatctgt tctcctttaa tcgaacgaac agcatgtaac tttttgattt
     9841 ctgaaaatgc ctgatatgcc tggctttcta cttgaaaatt taaaatgatt actcgtttgc
     9901 tcattaaaaa cgcccctttc ataccttctt gttatttatt ttacctttaa ttgaaaaata
     9961 agaaaagaaa agcactcttt ttttccaata aaatagggct gtgacaaaag tcgtatcaca
    10021 gctccttttt ccgaataaac ggcgttcttg aagctgatca cttctgtcac aacctccctt
    10081 aaatctgaat ctatattttt tggtatttgt ttttatttgc ttgcctattc gacaaattca
    10141 aattcaaact caccgatacg tacaaggtcg ccatctttcg caccgcgtgc ccgaagagct
    10201 tcatccacac ccataccacg taattgacga gcaaaacgca tcactgtttc atcatgatcg
    10261 aaattcgtca tttggaataa tttttcgatc ttctcaccag ataagatcca cgttgcatct
    10321 gaatcacgat cgatttggaa ctcaggacct tctggattga atccatagtg tacagtttct
    10381 tcttccagtt cttcttcgta caatgggaat tctggcgtta cttctaacaa atctgctgta
    10441 gcattcaata acgcgtccaa tccttgtcga gtcactccag aaattggaaa tactgggatg
    10501 tcatcagcaa attcatcttc tttttccttg ttcagctgtt ctttgaattt cactagattt
    10561 tccggtgcat ccggcatatc cattttgttt gccacgatga tttgcggacg ctctagcaag
    10621 cgtaagttat aagtagataa ttctttgttg atggcaagat agtcttcata aggatctcgt
    10681 ccttccatcc cactcatatc gatcacatgt aaaattactc gtgttctttc gatatgacga
    10741 aggaattgtg tacccagccc aacaccttgc gaagctcctt caatcaagcc tggcaagtct
    10801 gctgcggcaa aacttctgcc atcacttgta gtaaccattc ctagattagg tacgagcgtc
    10861 gtgaaatgat aagcgccgat ttttggacga gcagatgaaa taacggatag taaagtcgat
    10921 ttcccgacag atggaaaacc tacaagtcca acgtctgcca atactttcaa ttctagttcg
    10981 atttttcgct cttgtcctgg ttcaccgttt tcagcaattt ccggtgcagg attccgagga
    11041 gaggcaaaac ggatattccc tcgtccaccg cgtccacctt ttgccacaac tagtgtttga
    11101 ccattttcaa ttaaatctcc taaaagagca ccagtctccg catctcggac agtggttcct
    11161 tgtggtactt taacataggt atcttctgaa ccacgaccgt gcatcccttt actcatccca
    11221 ttttcacctg gctgcgcttt gaaatggcga ttgaaccgaa aatccatcaa tgtacgtaac
    11281 ccttcatcga cgatcaagat gacatcgccg ccacgaccac cgtctccgcc agccggtccg
    11341 ccgtcaggaa cgtatttttc gcgacgaaat gcaaccattc cgtctccgcc cttgccagct
    11401 ttcacatcga tcgttacttg atctaaaaac atggacataa ttaagtcctc ctattattca
    11461 tttatttatt tgactgcctg aatttccaca ataaacaaaa gtcttactta ttttatcggt
    11521 gaaatctgat tttgtctatc atttcagcaa aaagattgtt taccttcttc tttttttatc
    11581 atttggtaag gtttccaacc ttttttctgc taccaagtat aattttttga caaaatattc
    11641 tttgcgtagc agaataacag tgtattaatt gtattaattg aggatgccgt ttcacagcac
    11701 ccctaataaa ggaggaactt ttgatatgac agtaaaagta ggtattaacg gatttgggcg
    11761 tattggccgt ctggctttcc gccgcatcaa agaagtctca gatgatattg aagtagtagc
    11821 aatcaacgac ttaacaagtc caactatgtt agcacaatta ttgcaatttg actctactca
    11881 tggtacttat cctggcacag ttactgcaac agaagactca atcgtagtgg atggcgaagc
    11941 aactcgtgtt tatgcagaac ccgacgcaag caaaatccct tgggcaaaag aaaacggtgt
    12001 agatatcgta ctagaatgta ctggttttta tacatctgaa gaaaaagcaa aagcgcattt
    12061 agatgctggt gtcaaacgag tagttatttc tgccccagct ggtgctatga aaaccatcgt
    12121 ctataacgtc aatgatgata cattagatga aaacgacaaa atcatctcag caggttcatg
    12181 tacaacaaac tgtttagcac ctatggctta tttcttaaat aatgaatttg ggatcgaagt
    12241 tggtacaatg acaacagttc atgcctacac ttccacacaa atgctcttag atggacctgt
    12301 aaaaggcggt aatttgcgtg ctgctcgttc agctgctgac aatactatcc cacattctac
    12361 tggtgctgcg aaagcaatcg gcttagttat ccctgagcta caaggaaaat tacaaggaca
    12421 cgcacaacgt gttccagtcg ttgatggttc attaactgaa ttggtttcag tattgaaaac
    12481 aaaagtaact gctgatcaag taaacgaagc aatcaaaaaa cacacagttg acaatccttc
    12541 attcgggtat gatgaccgtc aaatcgtttc tagcgatgtc attggaacaa ctcaaggttc
    12601 gatctttgac ccaacgcaaa cagaagtaac aactgctggt gatttccaac ttgttaaaac
    12661 tgttgcatgg tatgacaatg agtatggatt tacttgccaa atgattcgct tactagaaaa
    12721 attcgctaac ttgtaatttg atagatcaat cgataaaaga agcctttgac tggatccagt
    12781 caaaggcttc tttcttttca tcccctactt ttaaactcgt tcaatgaaca tattggctta
    12841 aaaattgttg gactacttgc tgatattttt caggatctgt cttgtatgct tccgcatgct
    12901 cggctccttt tacaacatac ttttcttttg gtcccttcgt tgcacgatag accttatcta
    12961 acatttcaaa tggtacaaat gtatcttcgt ctccatgtat aaagagcatt gggcgctggt
    13021 ttttcttcaa ttgttccact gcatttgctt caccgaaaaa gtagccagca cggacttttg
    13081 tcactagact cgttacttgc ataagcggaa atgctggaag actaaacata tcgtctagct
    13141 gataagcaag ctcatcatga acggaagaat aaccgcaatc ttcaatgatt gctttcacat
    13201 tatctggtag tttttctccg ctagtcatca taactgttgc tgctcccatg ctgacaccgt
    13261 atagaacgat ttcttgcgat tttccattgt tttctagcac tttgtttatc cactgtacat
    13321 agtctttacg ctctggccat ccaaatccaa tgtaatcccc ttcgcttttt ccatgtcctc
    13381 ttgcatcagg aacaaggaca ttgtaaccca tatcatgaaa catctttgcg taattggtca
    13441 tcgtttcagc gtttcccata tagccatgag ccacaagagc tgttttttca cttttcgttt
    13501 cagcaggaag ataaatcgct gataatttca gaccgtcttt tgaagtgatg gtccaatcgc
    13561 ttcggttttc ttttgaacta aaccatttat gtgcatccgt ttctttggat gtctgtgttg
    13621 taccaggtgt atcttcgtct aaaaagtctt tctcagaagg cacaacagca taattataaa
    13681 aataattccc tgcaaacact aacgcaacaa tcagagctaa agcgacgcct aaaaggattt
    13741 tcatccaaag tttcataaat catccatctc atttctcttt tttcatataa ccttgaatta
    13801 gtataatcaa agaatctcta ctatgcaatg ttcattttta taacccgcga attgtaaaat
    13861 taagttagaa aaataaaaag gcatttatgg tacactcaaa ttgtatttcc gacgaaagaa
    13921 aacaaaggag tgtaaccata aatgacctac aaacatctta ccatagacga actgacaatg
    13981 atagaatcat attatcttca acataataag ccggttgaaa tcgctaaccg aatgggacgt
    14041 gctatacaaa ctatttataa tgtagtcaat aagttcaagc aaggtaagac tgctcttgat
    14101 tattggcacc agtataaaga aaataagaaa aaatgtggta gaaaagtcat tcaattacct
    14161 gctcatgaag tagattacat taaagagaaa gtcactcttg gttggacgcc tgacgtc
//
LOCUS       DAZKCC010000075        13979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000075 DAZKCC010000000
VERSION     DAZKCC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.077"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..461
                     /locus_tag="ACG8VH_002267"
     CDS             141..461
                     /locus_tag="ACG8VH_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110808.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="ACG8VH_002268"
     CDS             913..1113
                     /locus_tag="ACG8VH_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9110809.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="ACG8VH_002269"
     CDS             1260..4049
                     /locus_tag="ACG8VH_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP9110810.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="ACG8VH_002270"
     CDS             4096..4623
                     /locus_tag="ACG8VH_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP9110811.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="ACG8VH_002271"
     CDS             4644..5834
                     /locus_tag="ACG8VH_002271"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9110812.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8VH_002272"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8VH_002272"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9110813.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7967..8641
                     /locus_tag="ACG8VH_002273"
     CDS             7967..8641
                     /locus_tag="ACG8VH_002273"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9110814.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8638..8979
                     /locus_tag="ACG8VH_002274"
     CDS             8638..8979
                     /locus_tag="ACG8VH_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110815.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9009..9368
                     /locus_tag="ACG8VH_002275"
     CDS             9009..9368
                     /locus_tag="ACG8VH_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9110816.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9628..9750
                     /locus_tag="ACG8VH_002276"
     CDS             9628..9750
                     /locus_tag="ACG8VH_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110817.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9892..11976
                     /locus_tag="ACG8VH_002277"
     CDS             9892..11976
                     /locus_tag="ACG8VH_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9110818.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12287..13753
                     /locus_tag="ACG8VH_002278"
     CDS             12287..13753
                     /locus_tag="ACG8VH_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9110819.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4636 a         2430 c         2974 g         3939 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact tcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     5341 tcaatggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggga
     5401 attctgagat catcaatgga atgattgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccag
     5581 gtgtttctct tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatca catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     6721 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     6901 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacggacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggttgt gcgcctgccg ttcagctccg
     7201 agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt gtgacttgtg
     7261 gccggagagc aacgtttatt taaaaaaaag aactgcgaac tctcgcagtt ctttttttta
     7321 aatacgggat aatggaagtt tccagcagac aagagaagaa agcaaaagaa attgctattt
     7381 gattcacaag gttctataac gaaatcgcaa tcaatcaaac atttacaaag aaggtaattc
     7441 aaatggaaat ctgatttgat gatgcttctg ctgttgaaat aaagcagcat ctagcacctg
     7501 aaaacaaact aatagtaaca ttcgaagatg gagtaggtcc gtattttcag catgcaataa
     7561 tccatatgta agttcaattc acccttgaaa tcatcggacc agataaagtt atgaccaatc
     7621 tatcccaagc aatctagaag agatattagt aaagggacat ttactagaag tcttagatga
     7681 gcctataaat atatgtttcg atggcgtcat cagtggtctg attgacggaa gcttgggtgt
     7741 gatcgatctc tctggaccaa acaacgaagg aatcaagggg aatccggcac gttaagaaaa
     7801 aaatagcata aaaaactaga cacttgatga gcgttcattc aattgtttga aggaactagg
     7861 ctggtgtcat tttttataca tatattttct ctttcgtctt gatagggctt atgggataat
     7921 ataaggaaga aaatcatcgt tttattcaaa aaggagtttt tgacaaatga aaaaacgtat
     7981 attatttatc ggtgatagca ttacagacgc aggaagatac cgagcagaag caactgatct
     8041 aggtaaaggt tatccacttt tgattgccgg aaaactaatc tcccaatatc caacgatgga
     8101 atttgaatgc atgaaccgtg ggataggagg agacaaaatt gcagatctgc aagtacgctg
     8161 gcaagaagat tgtttagctt tacaacctga ctatgtatcg attttgatcg gaatcaatga
     8221 tacctggcat aatactagcg atcaggctat attcggaact gagaaatctg ctcagcaatt
     8281 tgaatccgtt tatcgaaaat tactaaatga acttcaaaca gcggaaatct gcaacatcat
     8341 cttgatggag ccgtttgtac ttcctataac cgacgaccgt catggctggc gtaaagactt
     8401 agaccctaaa atccaaattg ttcgtaaact tgctaaggaa tatggcgcat tgctggttcc
     8461 tttagatggt atattcaatg ctttagggat tgcttatggt ttttcgaaat atacaagaga
     8521 agatggggtc cacccaacga ttggcggaca tgagatcatt gcacaaagct ggatagatgc
     8581 aattgtcgca gtcgattttt ttagtggaga taaaaatgag cgaaagaaaa gtgaaaaatg
     8641 atggaaataa ggtgggcagt aaaagaagat ttacctcaaa tcaatcagct gattactgat
     8701 catccagaat cattcgtaaa agcaaaaacg gcagctgacc taaatgataa gaataaacta
     8761 ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat ttgcgcaagc ggacgtgcaa
     8821 cagtcagaag aacgattgga tcagcgacca tcaggaaaac taatccaaca cacattagta
     8881 acaaaagaaa atacggaaga aatccaaaaa aaatttgcag aatacttgat gctggaagct
     8941 aaaaaaagaa gaataaaatc aatttttgcc cagttttaat caaaaacagt aatagaagga
     9001 gaaatacaat gagttgttta ggaaatgtca tctggtttat attcggcgga ctaatcggtg
     9061 gtcttagctg gttagcagcg gggatacttt ggtgtatcac gatcataggt atcccaatcg
     9121 gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc ttttggcaaa gaagtaattt
     9181 atcaccagtc tggcatgtca ttgatcgtta atatcatctg gctgatcgtt tcaggcctac
     9241 caattgctct cgcacatgtc gtcagcggca tccttctatg tatcacgatc atagggatac
     9301 cttttgccaa gcaatctttc aaacttgctc gattggcgtt gatgccattc ggtgcagaaa
     9361 ttgtataaaa gagaataata aaggttagat aaagcataga gccaggtggg atacatgtat
     9421 ttcacctgac tctatgcgtt ttcctgtata agttcttcta tgtgactttt acgtctttta
     9481 tttatatggg gttactgttt atggaaagtt gaaataattt tcattaaaga actctttcta
     9541 tatttttgaa agcgtagtgg agcttcgtat atctgggaag gacattttgc agggattgaa
     9601 aaaagatatt gtcctatcga aaaattaatg aatttagaca aattgccagc aacaggcttc
     9661 aaacttagtt gctatcctgt caagatcaag aaggcaagtg cgggttggat aagagcagtg
     9721 gcaatgatcg aagagaaaaa gaaagaataa gccaaaacat ctgtatggaa gaaattttcc
     9781 gatgcagatg tttttcattt tttctttcgg ataccttgac atatgaataa ccattcattt
     9841 ataattagtt atgaagagat attcatatgt tgaggcgagg aggtcattaa aatgaaaagt
     9901 tacagattag aaggtttaga ttgtgcgaac tgtgcaatga aaatcgaaaa aggagttcag
     9961 aaaatcaatg gagtcaagga agcaacggta aacttcactt ctggaaaact aacgattgac
    10021 gcagaggaag atcatttggc tacgattgaa caagaaacaa aaaaagtagt gaaggaatta
    10081 gaaccagatg tcaaagtaac agaaatagat aaggaaaaag tatctgaaca tggaaatgaa
    10141 aaaaagagaa atacgctgtt ccggatttta ttttcccttg caggtatcgc tttattgcta
    10201 ttatttgatt ttaatgaacc aattcgcttg atagggtatt tattgattta tctgctgata
    10261 ggatatgatg tagtgaagaa ggcagtgatg aatatcgtca aaggaaaaat ctttgatgag
    10321 aattttctta tgtctgttgc tacgattggc gctatgttga tcggtgaata tccagaagcc
    10381 gtagcagtga tgctatttta tcaaatcggc gagtatttcc aaggattggc agtcagtcat
    10441 tcaagaaaat cgattcgcga attgatggcg atccgtccag aaacagctca cgtgcagacg
    10501 gcggaaggat tgatgaccgt gaaccctgaa gacgtgctga tcggtcagtt tgtacttgtg
    10561 aagcctggtg aacgtgtacc gttagatgga acgatcatcg aaggagaatc attagtcgat
    10621 acgtcagcac ttacaggaga atcagtccct aaatctgtat atgttggtga aacagtatta
    10681 agcggattta tcaataaaaa taaaccattt ttagttcaag tagaaaaatc ttatgaaaat
    10741 tctactatca gcaaattatt agaattagta gaaaatgcca gcagtaaaaa agcaccagct
    10801 gaaaatttca ttactaaatt cgcgcgctat tacacaccag tcgttgtagg attggccgtt
    10861 ttattagctg ttttacctcc acttgttgtt tctagagcgg cgttttctga atggatctat
    10921 cgagcattga catttttagt gatctcatgt ccatgtgcat tagtgatttc cgtgccactt
    10981 agtttctttg gcggtatcgg aggagcaagt aaaatcggtg tattagtaaa aggttcaaat
    11041 tacttggagt tgcttgcaca aactgagaca gtggtattcg ataaaactgg cacattaacg
    11101 aaaggtgact tttcgatcca gacgatcgat ccaacaggaa ttgatccaaa gaaatttatg
    11161 caatatgttg caagtgcaga acagttttct acacatccga ttgctcaatc tgttttagaa
    11221 ggctacgcag gtccgctttt accaacagct aatatccagg aatttgcagg tgaagggatc
    11281 ttagctgaag ttgatggcaa acaagtatta gtaggtaatc acaaattgat ggaaagattt
    11341 gaaatctctt ttccttcaag tcaagaaatc ggtacattgc tttatctagc gatcgatcaa
    11401 agttacagtg gttatcttgt tattgcagat accttgaaag aagatgccgt agacgcattg
    11461 gttcaattaa aacaggctgg tgtgaaaaac acagtcatgt tgacgggcga ctcgaaaaaa
    11521 attgctgatc atatcgggaa gcaagtaggc gtggacaaga tatatagcga attgcttcca
    11581 gaagataaag tgcagcggtt agaggaaatc ttacaaagta acaataaaaa gaccgcattt
    11641 gtcggagatg gaatcaacga tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg
    11701 ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa
    11761 ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa cattgaaaat cgtcaaacag
    11821 aacattgtct ttgctattgg gataaaaatt ttggtacttt ccttaggtgc ttttggtttt
    11881 gcatcaatgg gagatgcagt atttgcagat gttggggtga cagtattagc tgttttgaat
    11941 gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt
    12001 cctctttttt ttatatcctt ttcttaatga aaaatgtaaa gaaaatagat aggattataa
    12061 aataactaat gtttttaatt ttgcagaaaa ttcaaaatag cttgaaattc aataaggtca
    12121 atgaaataaa agaaaataac ttattttttt gatggaaaaa atatgaaaaa acgatgtgta
    12181 ttaaataaaa gaatacacac ataaaataac actgtcttgt aagtttgtta aaaattgaat
    12241 atgattacat ctggataaca aatttttatc ataaaggaga gaacgaatga acaacaaaca
    12301 attacgctgg tttactgttg cgttaattgc atttaacatg gtttggggca tggggaatgt
    12361 agttaataac tatgctcagc aaggagtatc agttgtcacc tcttggctat tgattttggc
    12421 catttatttt attccatacg cattgatcgt tggacaatta ggatcagcct tcaaggatag
    12481 taaaggaggc gtcagttcat gggtcgaaaa tacgacatct aataaacgac tggcatacta
    12541 tgccgcttgg acttactggg tcgtccatat tccttatttg gcacaaaagc ctcaagcaat
    12601 tctgattgct ggtggttggg cagtccaagg aaatggaaat atcgtaaata cgatgtcagt
    12661 acaaatggta gcagttattt cacttattat atttcttgcc tttttatatt tatcaacaaa
    12721 aggactttct actttgaaag tgattggcgg actggccgga acagcgatgt tcgtcatgtc
    12781 gatcttattt attcttttag ctgtaacggc accgttcatc aatccaacga tggaatttgc
    12841 cacaccacat atgaatcaag tgaaaacata tattccaaca tttgatttca gttatttcac
    12901 aaccgtttcg atgctagtat ttgcagttgg aggggcggaa aaaatatccc cttatgtcaa
    12961 ctctacaaga aatcctgcaa aggaatttcc taaagggatg atcttcttag cagcaatggt
    13021 agggatttgt gctgtattag gctcagtcgc aatgggtatg ctgtttgcta gcgataatat
    13081 ccctaaagac ttgatggcaa atggggcata cagtgctttt caaattttag gtaattacta
    13141 tggtatcggt aactcattga tgatcattta cgcattgaca aacatgattg gacaaatctc
    13201 cgcattagcc ttttcaattg atgcaccact aaaaatcttg ttagcagatg cggatccaga
    13261 atttgtacca aactggctac gtaaacgtaa tcgtaaaggt acattggtga atggttatct
    13321 tctgacaggt atactagtag gaatcattat tttgttgccg atctttggta tcaaagatat
    13381 gaatgaatta gtaaaatggt tgacgaactt gaactcagtt gtcatgccaa tgcgttacct
    13441 atgggtgttc ttggcatata tgatgttgaa taaagcttat aagaaattca attctgaata
    13501 taaatttgtt aaaaatccaa aaatcggttt tatctttggt gcttggtgct ttgccttcac
    13561 tgcgtttgca tgtatcctag gaatggtgcc aaaagtagat tatactgctg atcctaaagc
    13621 atggtggttc caattattca cgaatatcat cacaccaatt gtcttgatct tattaggtat
    13681 gatcttgcca gcaattgctc gtcgagacaa aaacaaagaa attactgaac cagaaacagt
    13741 ggttcagcct taaaatagaa atacttgtta caagaggttg tgacagaagc tacttctgga
    13801 acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
    13861 caaaaatagc tcctcatatt ttgccgtttt ttgagctaat gctgaaggtc agctgattga
    13921 acaccattta ttcgaaacaa gggactttga cacgactttt gtcacagtcc cttgttttt
//
LOCUS       DAZKCC010000076        13885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000076 DAZKCC010000000
VERSION     DAZKCC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.078"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG8VH_002279"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG8VH_002279"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9110820.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1708..2025
                     /locus_tag="ACG8VH_002280"
     CDS             1708..2025
                     /locus_tag="ACG8VH_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9110821.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2229..2594)
                     /locus_tag="ACG8VH_002281"
     CDS             complement(2229..2594)
                     /locus_tag="ACG8VH_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110822.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2802..3284
                     /locus_tag="ACG8VH_002282"
     CDS             2802..3284
                     /locus_tag="ACG8VH_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110823.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3481..4371
                     /locus_tag="ACG8VH_002283"
     CDS             3481..4371
                     /locus_tag="ACG8VH_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFP9110824.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            4696..7167
                     /locus_tag="ACG8VH_002284"
     CDS             4696..7167
                     /locus_tag="ACG8VH_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9110825.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7270..9006)
                     /locus_tag="ACG8VH_002285"
     CDS             complement(7270..9006)
                     /locus_tag="ACG8VH_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9110826.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9003..9707)
                     /locus_tag="ACG8VH_002286"
     CDS             complement(9003..9707)
                     /locus_tag="ACG8VH_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9110827.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9838..10341
                     /locus_tag="ACG8VH_002287"
     CDS             9838..10341
                     /locus_tag="ACG8VH_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFP9110828.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10301..10639
                     /locus_tag="ACG8VH_002288"
     CDS             10301..10639
                     /locus_tag="ACG8VH_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP9110829.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10660..12078
                     /gene="ffh"
                     /locus_tag="ACG8VH_002289"
     CDS             10660..12078
                     /gene="ffh"
                     /locus_tag="ACG8VH_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9110830.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12190..12768
                     /locus_tag="ACG8VH_002290"
     CDS             12190..12768
                     /locus_tag="ACG8VH_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9110831.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12772..13293
                     /locus_tag="ACG8VH_002291"
     CDS             12772..13293
                     /locus_tag="ACG8VH_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP9110832.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13372..>13885)
                     /locus_tag="ACG8VH_002292"
     CDS             complement(13372..>13885)
                     /locus_tag="ACG8VH_002292"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9110833.1"
                     /translation="DSYNKKVAEAFAKLLPEGYESVFVKIDDLPFYNEDLETPEQAPA
                     EWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHSVWDHKPGLVVTASP
                     GGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNIVEGTLSFFQTILDA
                     YLDFSNKLTE"
BASE COUNT         4611 a         2383 c         2782 g         4109 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat tttttcggta
     1681 tttaatattt aattaggagg gaatattttg cttttttata gtaattttat cttaatagta
     1741 gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaatgc aagtataggt
     1801 tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca aactattttt
     1861 tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt gaatattata
     1921 gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc aattattaca
     1981 caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag
     2041 ttttggtgat agtgatatta aaaatcagaa aagatagaaa aagcaggaga tagataaaat
     2101 gctgatattt cagcattttt taagagtata aaagagaaaa aaagtaataa atgtatacat
     2161 gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg
     2221 gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca taacgaccca
     2281 aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa
     2341 ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta
     2401 gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca
     2461 gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca
     2521 agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag
     2581 caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt agttttattg
     2641 taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa atgaataaac
     2701 tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag atagaaaatt
     2761 gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga aaagcagtaa
     2821 tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct tttacatggg
     2881 taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc ggatttgtgg
     2941 tttctaggaa ttttccagct ttgactgttt tctatttagt gataagtgta ggtctcttat
     3001 taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct tgcttattag
     3061 tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat ggtttgagcg
     3121 agagtggatt gaaggattca gcttattcct ccatgtattt cttgacgatc aaaccatggt
     3181 tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg acagaaatca
     3241 ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg atcatcagta
     3301 aaaaagcata tgagctgcgt aaaatgcatg tttctcatat gcttttttta ttattatgta
     3361 aagaatgcga aaaacgttta catgattttt acataactta actatatttt tacagcaacc
     3421 atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag gagcaataaa
     3481 atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg atgcggctca
     3541 ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc aaatgaacaa
     3601 gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc agcacaagaa
     3661 agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg aagcggaact
     3721 ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt atttgcagaa
     3781 gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt ggtcgggatg
     3841 gcgccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga attgattgat
     3901 atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca aaaaatcaac
     3961 gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg gggactggat
     4021 ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg gcagctggta
     4081 agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga ttccacacaa
     4141 gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa cagctgggga
     4201 atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt ccaaaaattc
     4261 ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt gggctattta
     4321 ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata actagcagat
     4381 aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc cacatataca
     4441 tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga gctaataccg
     4501 agggtcagct gattaaacac cgtttattcg aaaaaaagag ctacgacata gaactgtcgt
     4561 agctcttttt ttgctatatg acaggaatga aaggtgtttg caagcttaat gtgaggaaca
     4621 ctttccatat ctgttctata ctggatagga gaagtttgga aatatccaga aaacgaaatc
     4681 aatcgaggta gccacatgaa gaataaatgg ataaatcgaa gtaaaaaaaa gaaagtaaca
     4741 caaagatcat cagataaagt catccgtctt tttccatcgt ataaagaagg attaacggca
     4801 gatcaagtca aggaacgaat agaaaaaggt gcagcaaata attcagtcga tcccacattc
     4861 aaaacaaatc agcaaatcgt attggaaaac atatttacgt atttcaattt gatctttctt
     4921 atcttagcca ttttgctttg tttagtagaa tcctataaga acttgacttt tttaccagtt
     4981 atcatagcca atacaggaat cgccattttt caggaaattc gatccaaaaa gatcttggac
     5041 gaactaaatg ttttaaatac agcaaaagtc aatgtcatac gggacgggat agaacaaaca
     5101 atagatatag aagaccttgt aatagatgac attgttattt tagagactgg acatcaaatt
     5161 ccagcagatg cagaggtcgt ggaaggaaaa ctgcaagtta atgaggcttt attgacaggc
     5221 gaagcagatg aaataaccaa agaaataggc gatccattaa tgtccggcag tttcattgtt
     5281 tccggaaaag cttacgcccg attagacaaa gttggagcag attcgtatat ttcccagctg
     5341 acagtcaaag ccaagacaat gggagaagga gaacagtcgg agatgattgc ttctcttaat
     5401 caattgatca aatggattgg gatcatcatt attcctatcg gtatcctgct tttttctcaa
     5461 agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg ttgcaatgga agcagcattg
     5521 atcggtatga tacctgaagg actgtacttg ctgacgacga ttgcattagc attaagtgcg
     5581 actaaactag caaagcaaag agttctttta cacaatatga aaagcattga gacattggca
     5641 agggtaaatg tcctttgtgt agataaaaca ggaacgatca cagaaaataa aatgtctgtt
     5701 caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc agtcagatca attggaagca
     5761 tgcattgctg actatgcaaa agcgatggca ggagacaatg caacaatgga agctgttaaa
     5821 gcatatttta caaaaaccac tgatcaatcc tatcaccatg ttattccttt ttcttctgtc
     5881 caaaaattca gcagtgtagc gttggcagac aaggtgtatg ttttaggagc gcccgaaatg
     5941 gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg tttctttcgc tgaaaaaggc
     6001 tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg aagaaccagt attgacagaa
     6061 gcagtcacac cattaggcta tattctgatt gctaatccta tcagaaaaga agcaagagcc
     6121 acatttgatt acttcaaaaa acagcaagta gccatcaaag tgatttctgg agataatccg
     6181 ctcaccgtat ccaatgtagc tgtccaagca gggatttcac atgctgatca gtacgttgat
     6241 gcatctaaat tatcagaaga agaatatgaa gcagctgtcg aaaaatatac agtttttgga
     6301 cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga taaagcgtaa caatacagta
     6361 gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga ctgcagtata
     6421 gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt acttgattct
     6481 gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa caatattgag
     6541 cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc actgttttca
     6601 gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat tagtttgttt
     6661 actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg aatcagaggg
     6721 aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga tatgatcgta
     6781 gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga tgtttctaca
     6841 gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat ctgttcaccg
     6901 ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt tttttcggtc
     6961 atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact tttgctgttg
     7021 agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt ggtagaatat
     7081 ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagc taaaaaattt
     7141 ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt aaaaaagaac
     7201 aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg cacgagcagt
     7261 tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc caagatgact
     7321 gtctacagta atcgtaccat taaggagttg cacgtagtct tttacaatcg ctaaccctaa
     7381 tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg cggtaaaaac gctcaaagat
     7441 ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa acgatagatc
     7501 atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt ggacagcatt
     7561 ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa atggttcgtt
     7621 ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat aattttgaac
     7681 gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat tctcgccatc
     7741 ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat ctttttgcat
     7801 gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca gtaaagtctc
     7861 ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt tgccaacgaa
     7921 atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca cccctagaat
     7981 ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg tctgccgacc
     8041 ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt gatgaatcag
     8101 ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt gatagcttac
     8161 cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat cagcatcatc
     8221 aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct cttctgtggt
     8281 agtacacgcc cgatacatct tactcatttg ttcgctcaac gcattaatcg tctgatacaa
     8341 ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat tttgcaccat
     8401 tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga tcagataata
     8461 gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc aacggcgaaa
     8521 actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa tggcttctaa
     8581 ctggccactt tttttgactg gtaatgcgac atataaaaga tcctcattca aagtagtact
     8641 tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt cgggacggga
     8701 gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc cagtagcatc
     8761 cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag ttttgttttg
     8821 cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt tttttgtcag
     8881 gtagctttct tgctgttcca cgatctgctg ttgaaaataa tgggaaatca attgccagcc
     8941 accaaaaaac aaactaacta aaagaataag aaagaagaga cgttcataaa tttttttcca
     9001 tttcatcgtt ttggttcctg aaacttatag ccaaatcctc taatagtcac caagtacttt
     9061 ggatgttttg gatctcgctc gattttttct cgcaaatgac taacgtgtac atctacgatt
     9121 cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc tcggtcgatc
     9181 acacggtctt ttcgtttcat gaaataaaca agtaattcaa attctttcgg tgttagttca
     9241 atcagatggt cgtctacggt cacttgataa ttggtcaaat cagcagtgat ttgtccaatc
     9301 gatagatatt caggctcagc ttcatctttt gattgcttac gaggttcaat ccgtctgaat
     9361 attgctttca tacgggctaa tacttcgcga ggactaaatg gtttggtcaa atagtcgtca
     9421 gcacctattt ctaggccaat gatccgatcc acttggtcat ctttagccgt cagcatcaaa
     9481 atcggtgttt catttttttc ttgacgcagc cgctgagtga tctccatgcc atccatcgtt
     9541 ggaagcatca catctaaaat gatgaaatca aatgattgtt ctaatgctaa ttctaatcct
     9601 tcgcctccat ctgtggcact ggttaccttg tagccttctt tttccaaatt gaacgtcaag
     9661 agcgttacaa tagaaggttc atcatcaaca actagtattt ttttcattca aatctccttt
     9721 aaataggttt cattctgttt atattttagc atagaatcag tacaaagggc tattttacaa
     9781 aagaaataaa tggtataata ggaaggcact ttgctgaata gaaaggaaga gcagtcaatg
     9841 attggaatca gtgcatgttt aggcggtctg gcctgtcgct atgatggaca gaaaaaagaa
     9901 gtaccagaat tgatggaact agttgaaact ggagaagctg tcatggtctg tccagaagtg
     9961 atcggcggat taccaattcc tagagatccc gctgaaatca ttggtggaaa tggattcgat
    10021 gtgtggaaag atcaagcaaa agtatggacg acaagcggcg aagatgtaac ggaagcgtac
    10081 aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga tctagtcatc
    10141 atgaaggaaa aaagcccttc atgcggaacc cattcgattt atgacggaag tttttccgga
    10201 ataaaaaaaa atggtgccgg agtggctact gcttatttta ttcagcaaaa aatgactgtg
    10261 ctctctgatg aagaatggct gaaaactaga ggtgagcaga atggaaatcg aaaagaccaa
    10321 tcgaatgaac gcgctctttg aattttattc gaccttattg acagaaaaac aaatgaacta
    10381 catggaatta tattatgcag atgatttttc attaggtgag atcgcggaag aatacgaagt
    10441 aagccgacaa gcagtatatg acaatatcaa gcggacaagc aagatcttgg aggattatga
    10501 aaaaaaactc catctttttt ctaattatat tgttcgagaa caggtattgg aaaatatgac
    10561 tgcatatatt acagataagt acccggaaga taaaaaacta cttgaatatg tacagcaaat
    10621 tcaagcaatc gaagaataaa taaaaggaat gaagaaaata tggcatttga aagcttaact
    10681 gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag gaaaagtatc agaagctgac
    10741 gtaaaagaaa tgatgcggga aatccgcctt gccttactag aagccgatgt taacttgcaa
    10801 gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg taggggcaga agtgctggaa
    10861 agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg aagaattgac gatcacactt
    10921 ggttctgaaa cagcagagtt aaataaatca ccaaaaatac caactgttat catgatggct
    10981 ggtcttcaag gggcaggtaa aacgaccttt actggtaaat tagccaatta tttaaagaaa
    11041 aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat atcgaccagc tgcgatcgac
    11101 caattgaaag tattaggtca acagctagac gttcctgtat ttgatatggg aactgaagtc
    11161 agcccggtag agatcgtccg tcaagggatg gagttagcaa aagaaaagaa aaacgactat
    11221 gtattgatcg atacggcagg acgtctgcat atcgatgaaa cattgatgga tgagttgaaa
    11281 caaatcaaag aattgacgca accaaatgag attttgcttg ttgtcgatgc catgactggt
    11341 caagatgccg tcaacgtagc tgatagcttc aaccagcaat taggtattac aggggtcgtc
    11401 atcactaaac ttgatgggga tactcgaggc ggggcagcac tatcgatccg ctcagtgaca
    11461 ggcgcaccaa tcaaattcat cggttctggt gaaaaattga ctgacttaga agtattccac
    11521 ccagatcgta tggctagccg tatccttggt atgggggata tgctgacatt gattgaaaaa
    11581 gcgcagcaag actacgacga gaaaaaagcg gaagaacttg cacagaaaat gcgggaaaat
    11641 tcattcgatt tcaatgactt tattgaacag cttgatcaag tgatgggtat gggcccactg
    11701 gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc ctggaatcga aaacgtcaag
    11761 gtagatccaa aagatgttga acgcaaaaaa gcgatggttt attctatgac gccagcagaa
    11821 agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc gaattgctgc cggttctgga
    11881 aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca aggaatcaag aaaaatgatg
    11941 cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg accaaatgtt tggttcaggc
    12001 gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga tcaagaaaaa taagaagaaa
    12061 aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta cagactgaaa atgtctactg
    12121 ccggagcttt tctatcagca atttttattc tttactaata caattactct aaacgaagag
    12181 gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa gtatgcggca atatcatgat
    12241 actgtctggg gtgttcctga atatgatgac caaaagctgt tccgaaaatt gatgcttgat
    12301 attaatcaag ccggattgag ctggcagacg attttaaata aaagtgaagc attcgatgaa
    12361 gcatatgatc gattcgatat caaaaaagtc gcaaattatg gagaaacaaa aatcagtgaa
    12421 ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa tcgaagcagc cgtccaaaac
    12481 gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt tttcagagta tctttggtcg
    12541 ttttctaatg gaaaaatcgt tgattcttct tatcattcac aagaagaagt cccaacaaca
    12601 agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa gaggatttaa atttatcgga
    12661 agcacaacaa tctatgcttt tttagaagct gtcgggatca tcaatgatca ccttgacagt
    12721 tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg gcgaataaag catgcgaaaa
    12781 atcatatttt atggtgctat aagtttagat ggctatttgg caacgaaaac ggatgatctt
    12841 caatggcttt ttgacactcc aacaggtaag caaactactt atgaagcttt ttatgaaaca
    12901 atcgatacga ccatcatggg cagaaaaact tatcaggaag caaaaaaata tttggagacc
    12961 gaagcaattt atccagagaa aaagaatttc gttttttcca cccacgcaca attacaattg
    13021 ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc taaaaaatct acaaatgaca
    13081 gaaggaaaag atatctgggt tgtgggtggc ggtcgattga tcaaaccgct attggaacat
    13141 caaatgattg atgaatggta tatccagatt acccctgtat tattagggga cgggattcct
    13201 ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc tagacacgac acgattcggt
    13261 gaatttatcg aattgcatta tgtacgaaag tagaagtaaa taaaataaaa aaggcaacta
    13321 tatgcagaaa tgtcctgtac atagttgcct tttttattac cgttatgctt tttactctgt
    13381 taatttgtta gaaaaatcta gatacgcatc taaaatcgtt tggaagaaac ttaatgttcc
    13441 ttcaacgata ttaccatttt catcaattag attcgcaacg tttccgatat atgcttccgg
    13501 ttgctgcaag gttggcacat tcaaaaagac aagtgattgg cgtagatggt ggtttgcgcc
    13561 aaagccgccg atcccacctg gtgatgcagt aacgactaaa cctggtttat gatcccaaac
    13621 actgtgtcca taaggtcttg agcctacatc taaagcattt ttcaatactg ctggcacaga
    13681 acggttgtat tcaggagtaa cgaaaacgac accgtctagt cccctcactt cctcacgaaa
    13741 acgagtccat tcagcaggag cctgttctgg agtttcgaga tcctcattgt aaaatggcag
    13801 atcatcgatt tttacaaata cactttcgta cccttcagga agcaatttcg caaaagcttc
    13861 tgctactttt ttgttgtaag aatct
//
LOCUS       DAZKCC010000077        13858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000077 DAZKCC010000000
VERSION     DAZKCC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.079"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..2281
                     /gene="acm"
                     /locus_tag="ACG8VH_002293"
     CDS             116..2281
                     /gene="acm"
                     /locus_tag="ACG8VH_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP9110834.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2553..3038)
                     /locus_tag="ACG8VH_002294"
     CDS             complement(2553..3038)
                     /locus_tag="ACG8VH_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP9110835.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3128..3473
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3518..4159
                     /locus_tag="ACG8VH_002295"
     CDS             3518..4159
                     /locus_tag="ACG8VH_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9110836.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4178..4813
                     /locus_tag="ACG8VH_002296"
     CDS             4178..4813
                     /locus_tag="ACG8VH_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9110837.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4831..5646
                     /locus_tag="ACG8VH_002297"
     CDS             4831..5646
                     /locus_tag="ACG8VH_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9110838.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5965..7401
                     /locus_tag="ACG8VH_002298"
     CDS             5965..7401
                     /locus_tag="ACG8VH_002298"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9110839.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7590..10037
                     /locus_tag="ACG8VH_002299"
     CDS             7590..10037
                     /locus_tag="ACG8VH_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9110840.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10267..11334
                     /gene="rlmN"
                     /locus_tag="ACG8VH_002300"
     CDS             10267..11334
                     /gene="rlmN"
                     /locus_tag="ACG8VH_002300"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9110841.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11324..11530
                     /locus_tag="ACG8VH_002301"
     CDS             11324..11530
                     /locus_tag="ACG8VH_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110842.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11527..12630)
                     /locus_tag="ACG8VH_002302"
     CDS             complement(11527..12630)
                     /locus_tag="ACG8VH_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9110843.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13183..13731
                     /gene="aac(6')"
                     /locus_tag="ACG8VH_002303"
     CDS             13183..13731
                     /gene="aac(6')"
                     /locus_tag="ACG8VH_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9110844.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4746 a         2301 c         2937 g         3874 t
ORIGIN      
        1 ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt
       61 ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa
      121 aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa catcactgat
      181 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
      241 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      301 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      361 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      421 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      481 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      541 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      601 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      661 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      721 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      781 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      841 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      901 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
      961 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
     1021 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
     1081 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
     1141 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1201 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1261 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1321 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1381 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1441 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1501 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1561 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1621 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1681 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1741 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1801 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1861 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1921 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     1981 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     2041 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     2101 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     2161 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2221 tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta
     2281 gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt
     2341 ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg
     2401 gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac
     2461 attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc
     2521 ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt
     2581 tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg
     2641 atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg
     2701 atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct
     2761 aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca
     2821 gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt
     2881 ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca
     2941 tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt
     3001 agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt
     3061 attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa
     3121 gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg
     3181 ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg
     3241 tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa
     3301 ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt
     3361 gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt
     3421 ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg
     3481 ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat
     3541 gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg
     3601 ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg
     3661 ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat
     3721 ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact
     3781 cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca
     3841 atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac
     3901 ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga
     3961 agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat
     4021 gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc
     4081 aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag ttgaaggaaa
     4141 attaaattat taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa
     4201 caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa
     4261 aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc
     4321 tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt
     4381 cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc
     4441 acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa
     4501 agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt
     4561 gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcta ttgccagagc
     4621 attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga
     4681 attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat
     4741 tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc
     4801 cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg
     4861 atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg
     4921 gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg
     4981 ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc
     5041 tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt
     5101 gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga
     5161 gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct
     5221 aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat
     5281 gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa
     5341 aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga
     5401 cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac
     5461 gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt
     5521 gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag
     5581 aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga
     5641 aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca
     5701 gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt
     5761 aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa
     5821 gccattttca caaaattttt cttactactc gaaactaagc gactttttca caacctcctt
     5881 tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga
     5941 aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat
     6001 tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg
     6061 aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg
     6121 aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa
     6181 gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga
     6241 cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt
     6301 gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa
     6361 gatgaatata atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa
     6421 acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat
     6481 atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa
     6541 cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt
     6601 cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca
     6661 attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat
     6721 tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa
     6781 gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa
     6841 ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac
     6901 ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga
     6961 gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg
     7021 gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg
     7081 ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa
     7141 atccatgacg actatcgaat caattactta caaagccacg tcaaagccat caaagaagca
     7201 atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc
     7261 tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa
     7321 gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa
     7381 gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc
     7441 cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa
     7501 atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata
     7561 atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa
     7621 ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg
     7681 gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag
     7741 gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc
     7801 ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta
     7861 aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg
     7921 cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt
     7981 tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg
     8041 gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag
     8101 cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt
     8161 taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca
     8221 agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag
     8281 aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga
     8341 aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata
     8401 cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg
     8461 cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt
     8521 cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg
     8581 aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc
     8641 aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa
     8701 gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg
     8761 aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg
     8821 cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga
     8881 ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag
     8941 cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac
     9001 gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta
     9061 gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc
     9121 atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca
     9181 tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa
     9241 aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata
     9301 tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa
     9361 ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca
     9421 atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata
     9481 tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg
     9541 taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt
     9601 ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga
     9661 ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc
     9721 aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag
     9781 ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac
     9841 ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt
     9901 acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag
     9961 aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg
    10021 acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa
    10081 gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta
    10141 tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt
    10201 attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa
    10261 aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt
    10321 atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa
    10381 cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg
    10441 gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca
    10501 gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa
    10561 gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt
    10621 tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag
    10681 atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc
    10741 gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat
    10801 atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt
    10861 ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt
    10921 tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc
    10981 ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc
    11041 acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta
    11101 gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca
    11161 gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat
    11221 gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat
    11281 gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat
    11341 cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa
    11401 cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac
    11461 cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc
    11521 aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc
    11581 tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat
    11641 atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt
    11701 catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa
    11761 caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc
    11821 gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat
    11881 agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc
    11941 cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg
    12001 cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc
    12061 cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc
    12121 atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat
    12181 gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt
    12241 atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa
    12301 gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc
    12361 gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt
    12421 tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa
    12481 agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac
    12541 ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt
    12601 ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg
    12661 gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct
    12721 aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc
    12781 taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag
    12841 tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt
    12901 attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat
    12961 aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt
    13021 tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat
    13081 gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt
    13141 caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt
    13201 gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg
    13261 gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc
    13321 gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac
    13381 ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa
    13441 ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg
    13501 atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat
    13561 gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc
    13621 tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa
    13681 ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag
    13741 gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg
    13801 aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac cgtgtacc
//
LOCUS       DAZKCC010000078        13429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000078 DAZKCC010000000
VERSION     DAZKCC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.080"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8VH_002304"
     CDS             complement(<1..914)
                     /locus_tag="ACG8VH_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP9110845.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8VH_002305"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8VH_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9110846.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG8VH_002306"
     CDS             2006..2176
                     /locus_tag="ACG8VH_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110847.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACG8VH_002307"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACG8VH_002307"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9110848.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACG8VH_002308"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACG8VH_002308"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9110849.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACG8VH_002309"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACG8VH_002309"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9110850.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACG8VH_002310"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACG8VH_002310"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9110851.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACG8VH_002311"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACG8VH_002311"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP9110852.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACG8VH_002312"
     CDS             6751..7752
                     /locus_tag="ACG8VH_002312"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9110853.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACG8VH_002313"
     CDS             7749..9110
                     /locus_tag="ACG8VH_002313"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP9110854.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACG8VH_002314"
     CDS             9070..10221
                     /locus_tag="ACG8VH_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9110855.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACG8VH_002315"
     CDS             10196..11365
                     /locus_tag="ACG8VH_002315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9110856.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACG8VH_002316"
     CDS             11540..12919
                     /locus_tag="ACG8VH_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9110857.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACG8VH_002317"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACG8VH_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZKCC010000079        13404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000079 DAZKCC010000000
VERSION     DAZKCC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.081"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..606)
                     /locus_tag="ACG8VH_002318"
     CDS             complement(64..606)
                     /locus_tag="ACG8VH_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP9110858.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="ACG8VH_002319"
     CDS             741..1130
                     /locus_tag="ACG8VH_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110859.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="ACG8VH_002320"
     CDS             1134..1898
                     /locus_tag="ACG8VH_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9110860.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="ACG8VH_002321"
     CDS             2027..2974
                     /locus_tag="ACG8VH_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9110861.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="ACG8VH_002322"
     CDS             3104..3844
                     /locus_tag="ACG8VH_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110862.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4135..4803)
                     /locus_tag="ACG8VH_002323"
     CDS             complement(4135..4803)
                     /locus_tag="ACG8VH_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9110863.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4937..5224
                     /locus_tag="ACG8VH_002324"
     CDS             4937..5224
                     /locus_tag="ACG8VH_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110864.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5640..7178
                     /locus_tag="ACG8VH_002325"
     CDS             5640..7178
                     /locus_tag="ACG8VH_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP9110865.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7175..8140
                     /locus_tag="ACG8VH_002326"
     CDS             7175..8140
                     /locus_tag="ACG8VH_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110866.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8127..9449
                     /locus_tag="ACG8VH_002327"
     CDS             8127..9449
                     /locus_tag="ACG8VH_002327"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9110867.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9512..9616
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9690..10433
                     /locus_tag="ACG8VH_002328"
     CDS             9690..10433
                     /locus_tag="ACG8VH_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9110868.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10448..11440
                     /locus_tag="ACG8VH_002329"
     CDS             10448..11440
                     /locus_tag="ACG8VH_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9110869.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11452..12189
                     /locus_tag="ACG8VH_002330"
     CDS             11452..12189
                     /locus_tag="ACG8VH_002330"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110870.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12179..12901
                     /locus_tag="ACG8VH_002331"
     CDS             12179..12901
                     /locus_tag="ACG8VH_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9110871.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12985..>13404)
                     /locus_tag="ACG8VH_002332"
     CDS             complement(12985..>13404)
                     /locus_tag="ACG8VH_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9110872.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4416 a         2294 c         2786 g         3908 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3901 ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg agtttaagcg
     3961 aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa ctattttcgc
     4021 agatcgtttg caaaaatagt ttattttttt ataaagaaga ttttactgaa taatatcatt
     4081 ttagtttaca taatgatatt tctgtaaatt aaataaagta gtgaaaaata agaatcagtg
     4141 ctctaactgt tcaagaggat caagtggttc aaatgtttct gtagtctgtt ttgcttccaa
     4201 ttggggcaaa acagatccaa tttccagata cttcacttta ttcaatgtat aagatagaat
     4261 agaacagcta agtaatgtat acagaaaatg tgtgagatcc aaataaagca aggagacaac
     4321 taaaatacca aaagtaatca agaaaagact tttgccaatc ttaatatgaa aatatttttc
     4381 aatggcttga taaatgatat ctgttccgcc tgtgcttccg ccgaatcgaa agacgagacc
     4441 gcaaccaatg ccagaaagaa tacctgtcat caaactattg atcagtagac tatctacagc
     4501 aaaatgtata tgaaggctct cccataaatg taaaaagaaa actagcgcaa atgttccgac
     4561 gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat gagataagca ataaaggtaa
     4621 attaaaaagc agtgaagtga cagaaggagc gaatccaaaa agattatgca aaagcaaagc
     4681 aataccagta accccaccat ctgccagctt agatggaata ttgattcctg tcacagctaa
     4741 tccgtagatt cctgcgccta aaatgattcc gcacaattct gtaaataaat ttaatttttt
     4801 catttctagc ctcttttctt ttgaaaattt tcagcaactt ctttattata ctgacttata
     4861 ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg ctgtttcttt ttatttatca
     4921 aataaggagt ggaaaaatgc gagcagcaga cttattgaca attcttacaa aatcacccaa
     4981 aaaaacagag tatcaaggag tttttttggc tcaagaacaa aaactggtgc ctgtgacaat
     5041 ggtccagata aataaagcaa atcaatttat ctttttattt gaacacggca aagcaccact
     5101 tgctatgaaa gacatctttc tttttttgat gaagaaccgt gagaaagctg tttattatca
     5161 gtcaaaagaa gaactcattc ctctttatgg agtgaaagag atagaaaata aagtggtgat
     5221 ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa acaaccattt tttacatttt
     5281 tggcaaaaaa gtgtcatgat acagtataat attcgttgaa caggccaata aaggtgttct
     5341 cccccacgag atttctactt tattggctgt ttctttttat taaaataaaa gttgtgtgtg
     5401 taaaaaagat atagctgatt ccaagtgaca atttcgtata gatttttatg gtattcgtag
     5461 tgaaaaaacg agaaccaaaa agttgatccg actttgttgc ttggattctt tttttgtcag
     5521 catatttaga ggtatttttt tctttttttt gttcgtgctg taaggttaag gcaagacaag
     5581 ttagttggga aaaaggaaag aaatgtttat acttaaagaa taacagaaag gaaggtcgca
     5641 tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga gttgctggaa gctgcatttc
     5701 aacatctgtc catttctctc atttctttgc tgattgcagc actgattgct attcctttag
     5761 cgatttgggc agccaatcat aaaaaaatgg cagaagtctt attgcagatc acgggtgtgc
     5821 tccaaacgat tccttcactt gccttattag gtttacttat cccatttgta gggatcggaa
     5881 ctgttccagc attgattgca cttgttattt acgctttgct tccgattttc caaaacacgt
     5941 atattggtct agctgaaatt gacccgtcta ttgaagaagc agcggtagca tttggtatgt
     6001 ccagaatgag acgcttgctg aaagtagagc ttcccatcgc attacctgtc attatctcag
     6061 gtatccgaac tgccttagtt ttaatcatcg gtacggctac tttggcagca ttgatcggag
     6121 caggtggatt agggacattt attttgttag gtattgaccg aaatacacct gttttgacat
     6181 tgattggtgc gatcagttct gccttgcttg caattatatt cagcggactg attcgctggt
     6241 tgcagcataa gaaacctcga tattccatca ttactttagc agcagtggtg tgtttatttg
     6301 gcggcgtttc tttatatcag tctgccttgt ttaaaaatga aaccatcacg attgctggaa
     6361 aattaggagc ggaacctgaa atcctgatcc aaatgtataa agaattgatt gaagacaaca
     6421 caaaaacaga agttgaatta aaaccgaatt ttggcaagac gagttttttg ttcaatgcac
     6481 tggatcaagg tcaaatcgat atctatcctg agttcacagg tactgtccta gaaagcttgg
     6541 tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa agaaaaaaca tatgacgaag
     6601 cagaagaatt attgaataag caattcgata tgacattatt aaaaccaatg gcttaccaaa
     6661 atacgtatgc cttggcagtc aagagatctt ttgcgaacga acatcaactt aaaaagattt
     6721 ccgatttggc agctatctct gatcagatca aagcgggatt cacactggaa tttattgatc
     6781 gttcagatgg atataaagga atccaagaag catatggctt gaatttttca agtgtacaaa
     6841 gcatggagcc aagtttgcgt taccaagcaa tcaataatgg agatgtcaat attgtggatg
     6901 cctattccac agatagccag ctcaaggagt acgatctagt gactctagaa gatgatcgag
     6961 ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa agattttgct aaagaacatc
     7021 ctgaaatcgt taaagcactg aataaattaa gcggaaaaat aacagaggat caaatgattg
     7081 atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac agaagtagca cgaatcttcc
     7141 tgaaaaatga aggattagta aaggaggaag atcagtgact aaattaatcg agttcaaaca
     7201 tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac ctaaatctgg caatcaaaaa
     7261 aggagaaatc tttgttttgg ttggtccttc cggtagtgga aaaacaacta ctttgaaaat
     7321 gatcaatgga ttatctaaac catcagcagg agacatctac ttcaaaggaa aatcgttaaa
     7381 tgaatataat ctgcagaaaa tgcggtggaa tatgggatat gtgctgcagc aaatcgcttt
     7441 gttcccgacc atgacagtca agcaaaatat tgaagtcatc cctgaaatgt taggttggga
     7501 aaagcagaaa agagcagacc gtgtagatga acttcttcaa aaagttggct tatctcctga
     7561 tatttatcga gatcgtatgc ctagagagtt gtctggagga gaacagcaac ggatcgggat
     7621 catccgagct attgctgcaa gcccagatgt gattttgatg gacgagccat ttagcgcatt
     7681 agatccgatt tcaagaaact cattacaaga attggttttg tccttacacg aagaattggg
     7741 cacgaccatc gttttcgtta cgcacaatat ggaagaagca atcaagctag gtgatcgtat
     7801 tgcatttatg aaggatggag aaattatcca atgtgacaca ccagagcagt tattgatgaa
     7861 tcctaaaaac gactacgttc ggcatttctt tgatgaaccg aaacagacga aagagtggcg
     7921 agtagaagat ttagtcgtca atggatattt cttaaatgaa ataccagaaa atgctcgtca
     7981 acaagtttgc tttgatacgc ctatgaaaga agtatattcg cttctttcgg tgtatccaag
     8041 cattgtgata gtagaaaagc aaagaagtat tgggtcgctg acaagtaaag aaatctttgc
     8101 ttttttaagt cgagaggagg aaggaaatga aaacatatga tgcaatcatc atcggttcag
     8161 gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc aggtaaaaca gtactagtgg
     8221 tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg atgtgatcct aaaaaagtct
     8281 tgttaagtgc agtagaggca cgtaatcggg taaaacaatt gagtggaaaa ggttttaacg
     8341 agatcccaac tgccaactgg gaagaacttc aaaaattcaa acgaacattt acggatccag
     8401 tcccagaaag ccgaaaaaaa caattagcag aagctgaaat cgatcatctt tcaggaaccg
     8461 ctcgtttttt ggatgattca tctattgaag tcaacgaaga agtcttccat gcggattact
     8521 tagttttggc aaccgggcaa agacctacga tcttaccagt ggagggaaag gaatacttga
     8581 aaactagtgc agacttttta tcattgcctg tactgcctaa agaaatcatt tttatcggtg
     8641 gtggttatat tgcttttgag ctagctacga ttgccaatgc tgcagggagc aaagtaacca
     8701 ttgtccatca taatcaacgg ccattaaaag aatttgaggc gtctttggta gaggaagccg
     8761 tccatcaaat ggaagcatct ggcattcagt ttgcttttgg tgttgaaacc caaaagatca
     8821 tttcagaagg tactcgctat cgtttagtcg gaaaagagac ggaattagta gcagatatga
     8881 ttttttgtgc gactgggaga cagccgaata cagaatcttt agcacttgag caagcgaata
     8941 tcgtctttga taaacacgga attgcagtca atgattatct gcaaacgagc aatccaaaaa
     9001 tttttgcgtg tggagatatt gtttcaagga aaaccccaaa attgacacca gtcgccactt
     9061 ttgaaggaaa ttatgtagct aagcggataa cggatgcaac aagcgaacct atcaaatatc
     9121 cgattatacc tacgatcgtc tacgccagtc ctaaacttgc tgaagtagga gttacaaaaa
     9181 gtcacgcctc ttcgtcagat caagtggtag agatggatct taccagttgg ttcacctatc
     9241 atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt tgaccagcaa aattatttga
     9301 ttggtgctgc agtgattagc gagcaagcag acgaattgat cgatgatttg acactcgtca
     9361 tcaatcaaaa attgactaaa aaagagttgg atagctatat catggggtat ccgacattgg
     9421 caagtgatct ctcctattta ttgaaataat cagcatgata actaaagcgc ttgttgcttt
     9481 ttccttcttg tgctacactt taaaaaatga atacctgttt ctggggatgt cgtagacgac
     9541 atgagagtaa gttgtgaact tagacccatt gaacctgtca gttaatactg gtgaagggag
     9601 aaacatagca gacgcttgaa gcgggttact atgtcgtgcc catttcaagc gttttttgtt
     9661 attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa tctttatcca gcagtcagta
     9721 tggcattcat tttaattatt tggcagattg ttgtcatagt ttggaaaata cctgccttta
     9781 ttttacccgg acctgctgct gtattttctg cattagcggc agatgcacaa gtattacttt
     9841 cccattctgt cacgacatta aatgaagcag tgcttggttt ggtaatagct agcgttcttt
     9901 cttttattac tgccttggca atggattatt ggaagttttt agaaatgtct ttttatccgt
     9961 tactagtgat ttcacaaatg ttgccaatca tggtgttagg gccattactt accctatggt
    10021 ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt gataagctat tttccgatcg
    10081 tagtttcttt ttcggataca ttgaggaaag tatcaaaaga gcaaatcatc tttttgaaaa
    10141 cgatgggggc agatacaggg aagatttatc gaatatataa aataccagcg ggtttatccg
    10201 gcttcttttc cggtttaaaa gtggctgcta cttattgtgt cagcggagca gtcgttggag
    10261 aatggctaag cgctcaagca ggcttagggt attacatgat acgcgtaaaa aacagctacc
    10321 agatcgataa agtttttgct gcgatcattt gtgtcattct attgagttta ttgctgaatg
    10381 gcgcttgttc attattgaaa aaagggtatt atcgtatgct ttatattttt taagagaagg
    10441 ggatcaaatg aaaaaattat ggcttttatt accgctactg ttgcttttat ccgcttgtgg
    10501 cactgcaaaa gaaacaagta gcaaacaaga aataaaggat ttgaaaaaag tgacacttgt
    10561 tttggattat gtaccgaata ctaatcatac tggcatctac ctagcaaaag aaaaaggcta
    10621 ttataaagag gctggtttaa atgtccaaat catcgaacca ggagataatt cgacaagtat
    10681 tggtctagta ggtgcggaca aagcacaatt tggtgtaagt tatcaagaag atgtcactta
    10741 tgctcacgca gatggacaga atataccagt caaagcaatt gctacagtga tcaaacataa
    10801 tacttctgga ttcgccaccc tatcagacag taacatccat tcaccaaaag attttgaagg
    10861 aaaaacatat gcaggttggc aaagtccaag tgaagaagct gtcttaaaag ctgtgatgga
    10921 aaaagacggc ggggactttt caaaactgac aatggtagga agcaatggag aagggccaga
    10981 aagcttagga aaatcctcag atatccaatg gtactttgaa ggttgggata tgatcaaagc
    11041 aaaagaagcg ggtattgaac taaactatat cccattgaaa gaattagatg aacggttaga
    11101 ttactatact ccggtaatta ttacgaatga tcaactgatc aaaagtgatc cagaattggt
    11161 acagtctttt atggatgcca cgaaaaaagg ctatcaggaa gctattaagg atccaaatga
    11221 cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat cggacgtttt tagaagaatc
    11281 acaagcattc ttgtcaaaaa attatacgga tgatcctgag aattggggat tgatggaaga
    11341 aaaagtttgg cgcaattata cagcttttat gcaggaaaac ggattgatca agcaagatgt
    11401 accttcgcaa gaactattca ctaatcagtt tataaaatag gagtgataga tatgtcaatc
    11461 caaatcagcc acttgaatgt agtcattgaa aacctgaaaa tattagaaga catccatttc
    11521 actatcccta aagatacctt tacatcgatt gtcgcgccaa gcggagccgg taagtcaaca
    11581 ttgctgaaaa ctttaacagg agtccagcct atgacttctg gaacgatcaa agtagacgat
    11641 caaaaagtaa ctggtctcca tacagcattt agttatatgc cgcaagaaga tatgttactt
    11701 ccttggctga gcgtctatca aaacgttacg ttatatcaaa aaatcaatca tctaacaatc
    11761 aatgaagaac aagtaaggga ttatctggat atctttgggt taaacggcta tgaacatttt
    11821 ttgccggaac aattatctgg cgggatgaag cagcgaacgg ccttgttacg aacaatcatg
    11881 aatccttctt cttacttgct tcttgatgaa ccatttggag cgttagatgc tatgaccaga
    11941 gggcagatgc aagactggat gttgaagctg cctaaaaaag caaagcggac tactttactt
    12001 gtgacgcatg atatcgaaga agcaatttat ttatcagaca ggattcttgt gctttccgcc
    12061 cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa aacgacggtc acgtgaatgg
    12121 ctactgcagc aatcagagtt gaagcaaacg atttatcagt tattggcagg tgaattagat
    12181 gttaagtgat gctcattgtc atttagatgg gagtcagagt ttagcactcc ttcagcagga
    12241 acacacgatt ttgacgatca tcaattgtga ttcgccagaa gaatggaagg aaaacagaca
    12301 gcttgcagca agtaagactc aagccttaag ttacggtatc catccttgga aagcagatag
    12361 ctatacgttt gaacaagttg agcctttttt gaaaaaagca aggatcatcg gtgaaatagg
    12421 gttagataat atttggacga atgtaccgat gactacacaa aaaaaagtat ttgagcgaca
    12481 gcttgctttt gcagccataa atgagaaacc tgttgtactg cacacaaaag gatgcgaaaa
    12541 agagatctta aattatatcc aagcttaccc taatcactat ttgatccatt ggtattcttc
    12601 tttggactat caacaagact atattgattt aggctgttat ttcagcattg gtcttgattt
    12661 gaaaaagaat ccagcagtct ggcaactagc taaagcagtc ccaatcgacc acttacttat
    12721 tgaaacggat ggactacatg cagcagagtg ggctttaggg gggatttttt cagaaaaaga
    12781 ttatgtctca ctgcttgaag aacagttaaa gacaatcgct gagatcaaag agctatcagt
    12841 tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt ttcattagat taggtaaata
    12901 aaaaagagat gtgacacaac tagtgtcgca cctctttttg gctttctttg tataaaagta
    12961 aactgtttga ttaataaatg aatcttagta ccaaccgttt gcttcccaga atgctttcgc
    13021 cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt cttggtttgc
    13081 agcagagtag tcaccattta agtaagaaga atctaattgg taacgtccga tgtaacgtcc
    13141 gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg atccattctt ttgcagagct
    13201 tgtgcttgct ggagtagcag cagtttctgt tacagcagga gcaggttgtg tttcagcaac
    13261 aggcgctgct tgtttttccg tttgaaccgt ttcttgtaca acaggttgtt cagcttggac
    13321 tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc cttctgttgg
    13381 gattgttaat tgttgtcctg agta
//
LOCUS       DAZKCC010000080        13390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000080 DAZKCC010000000
VERSION     DAZKCC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.082"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1365)
                     /locus_tag="ACG8VH_002333"
     CDS             complement(46..1365)
                     /locus_tag="ACG8VH_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9110873.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1362..2015)
                     /locus_tag="ACG8VH_002334"
     CDS             complement(1362..2015)
                     /locus_tag="ACG8VH_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9110874.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2303..2823
                     /locus_tag="ACG8VH_002335"
                     /pseudo
     CDS             2303..2823
                     /locus_tag="ACG8VH_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2821..3540)
                     /locus_tag="ACG8VH_002336"
                     /pseudo
     CDS             complement(2821..3540)
                     /locus_tag="ACG8VH_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3565..3750)
                     /locus_tag="ACG8VH_002337"
     CDS             complement(3565..3750)
                     /locus_tag="ACG8VH_002337"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP9110875.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3977..5250
                     /locus_tag="ACG8VH_002338"
                     /pseudo
     CDS             3977..5250
                     /locus_tag="ACG8VH_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5357..6388)
                     /locus_tag="ACG8VH_002339"
     CDS             complement(5357..6388)
                     /locus_tag="ACG8VH_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110876.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6395..7225)
                     /locus_tag="ACG8VH_002340"
     CDS             complement(6395..7225)
                     /locus_tag="ACG8VH_002340"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9110877.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7222..8037)
                     /locus_tag="ACG8VH_002341"
     CDS             complement(7222..8037)
                     /locus_tag="ACG8VH_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9110878.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8034..8858)
                     /locus_tag="ACG8VH_002342"
     CDS             complement(8034..8858)
                     /locus_tag="ACG8VH_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFP9110879.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8848..9948)
                     /locus_tag="ACG8VH_002343"
     CDS             complement(8848..9948)
                     /locus_tag="ACG8VH_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9110880.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10126..10911
                     /locus_tag="ACG8VH_002344"
     CDS             10126..10911
                     /locus_tag="ACG8VH_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP9110881.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10944..11327
                     /locus_tag="ACG8VH_002345"
     CDS             10944..11327
                     /locus_tag="ACG8VH_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9110882.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11403..11534
                     /locus_tag="ACG8VH_002346"
     CDS             11403..11534
                     /locus_tag="ACG8VH_002346"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9110883.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11503..11739
                     /locus_tag="ACG8VH_002347"
     CDS             11503..11739
                     /locus_tag="ACG8VH_002347"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9110884.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11751..11837
                     /locus_tag="ACG8VH_002348"
                     /pseudo
     CDS             11751..11837
                     /locus_tag="ACG8VH_002348"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11817..12788)
                     /locus_tag="ACG8VH_002349"
     CDS             complement(11817..12788)
                     /locus_tag="ACG8VH_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9110885.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4069 a         2920 c         2315 g         4086 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
     1381 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
     1441 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
     1501 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
     1561 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
     1621 ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
     1681 gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact
     1741 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
     1801 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
     1861 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
     1921 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
     1981 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctataca
     2041 ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt
     2101 gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaaaa
     2161 aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt
     2221 ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc
     2281 accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga
     2341 taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc
     2401 gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat
     2461 tcgtattatc cgcaacggtt cctatacgac acgtactcag atgttgaaag tcaaagaaaa
     2521 gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt
     2581 ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc
     2641 gattctcata gaactgtacg aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt
     2701 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
     2761 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
     2821 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
     2881 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
     2941 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
     3001 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
     3061 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
     3121 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
     3181 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
     3241 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
     3301 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
     3361 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     3421 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     3481 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     3541 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     3601 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     3661 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     3721 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     3781 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     3841 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     3901 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     3961 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     4021 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     4081 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     4141 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     4201 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     4261 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     4321 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     4381 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     4441 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     4501 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     4561 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     4621 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     4681 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     4741 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     4801 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     4861 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     4921 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     4981 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     5041 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     5101 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     5161 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     5221 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     5281 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     5341 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     5401 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     5461 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     5521 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     5581 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     5641 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     5701 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     5761 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     5821 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     5881 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     5941 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     6001 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     6061 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     6121 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     6181 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     6241 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     6301 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     6361 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     6421 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     6481 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     6541 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     6601 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     6661 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     6721 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     6781 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     6841 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     6901 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     6961 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     7021 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     7081 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     7141 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     7201 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     7261 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     7321 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     7381 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     7441 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     7501 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     7561 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     7621 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     7681 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     7741 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     7801 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     7861 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     7921 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     7981 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     8041 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     8101 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     8161 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     8221 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     8281 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     8341 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     8401 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     8461 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     8521 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     8581 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     8641 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     8701 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     8761 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     8821 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     8881 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     8941 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     9001 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     9061 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     9121 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     9181 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     9241 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     9301 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     9361 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     9421 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     9481 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     9541 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     9601 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     9661 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     9721 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     9781 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     9841 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     9901 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     9961 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
    10021 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
    10081 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
    10141 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
    10201 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
    10261 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
    10321 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
    10381 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
    10441 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
    10501 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
    10561 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
    10621 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
    10681 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
    10741 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
    10801 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
    10861 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
    10921 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
    10981 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
    11041 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
    11101 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
    11161 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
    11221 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
    11281 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
    11341 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
    11401 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
    11461 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
    11521 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
    11581 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
    11641 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
    11701 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
    11761 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
    11821 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
    11881 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
    11941 aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat ccataagata
    12001 aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag caaatgagga
    12061 accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg cgctggcgtg
    12121 tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata gcagtggtac
    12181 ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc aatcgtcagc
    12241 agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg cacttcgcag
    12301 ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt atctaagccg
    12361 gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa tgtatccaca
    12421 tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc cagtacggta
    12481 ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc ctgaaccagt
    12541 gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac agcatccttc
    12601 tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt ttcaatctgc
    12661 ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc ctttaaataa
    12721 acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct taatactgtt
    12781 ccttccatat agatttttga attacacact agtttaaact aaaacacaca aggaaaaact
    12841 taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat agactggtca
    12901 aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat tttaatattc
    12961 taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca ctatatcact
    13021 agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat
    13081 tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac gctgagagta
    13141 aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt tatttcagat
    13201 taccttaaat gattccattt actttttatc tcattttttc ctactagtta gtatcaaata
    13261 ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca gctaaaacct
    13321 tgtcattaca gcaaaaaatc acgttatcca gactgtagac aaagtccatt ttatggatga
    13381 tgtctacagt
//
LOCUS       DAZKCC010000081        13306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000081 DAZKCC010000000
VERSION     DAZKCC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.083"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(469..1083)
                     /locus_tag="ACG8VH_002350"
     CDS             complement(469..1083)
                     /locus_tag="ACG8VH_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9110886.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1689..2066)
                     /locus_tag="ACG8VH_002351"
     CDS             complement(1689..2066)
                     /locus_tag="ACG8VH_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110887.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2366..3247)
                     /gene="galU"
                     /locus_tag="ACG8VH_002352"
     CDS             complement(2366..3247)
                     /gene="galU"
                     /locus_tag="ACG8VH_002352"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9110888.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3290..4309)
                     /locus_tag="ACG8VH_002353"
     CDS             complement(3290..4309)
                     /locus_tag="ACG8VH_002353"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9110889.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4942..5946)
                     /locus_tag="ACG8VH_002354"
     CDS             complement(4942..5946)
                     /locus_tag="ACG8VH_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9110890.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5959..7008)
                     /locus_tag="ACG8VH_002355"
     CDS             complement(5959..7008)
                     /locus_tag="ACG8VH_002355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9110891.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(7025..7846)
                     /locus_tag="ACG8VH_002356"
     CDS             complement(7025..7846)
                     /locus_tag="ACG8VH_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9110892.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7833..8612)
                     /locus_tag="ACG8VH_002357"
     CDS             complement(7833..8612)
                     /locus_tag="ACG8VH_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9110893.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8629..9099)
                     /locus_tag="ACG8VH_002358"
     CDS             complement(8629..9099)
                     /locus_tag="ACG8VH_002358"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9110894.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9111..9530)
                     /locus_tag="ACG8VH_002359"
     CDS             complement(9111..9530)
                     /locus_tag="ACG8VH_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9110895.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9611..12388)
                     /locus_tag="ACG8VH_002360"
     CDS             complement(9611..12388)
                     /locus_tag="ACG8VH_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9110896.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12579..12803
                     /locus_tag="ACG8VH_002361"
     CDS             12579..12803
                     /locus_tag="ACG8VH_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110897.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3918 a         2765 c         2156 g         4467 t
ORIGIN      
        1 ttataaatat ccattatcta tccttcaatt tttgtgtatt ttatttctat tagaaaaagg
       61 tatcctgttg gacaattttg ttatatcagg taaacttcat tgcaataatg ataacaaatg
      121 tactataatt actttgtaaa gagttttggg gaagactctt tcatcaacgt gtattaagag
      181 acaagaaaga atccatatgg attctttctt gtctcttttt ccactacttt ttatttttct
      241 gacactacat attcttataa ctcatttctc ataactgtga aacaacttat aaaaataaca
      301 aactttaaac aaaacaagct taaaatcaaa gatgatccat cttaaaataa ctaaaacaaa
      361 aaaagtagtc gttgttaatc ctcattaata atcgttttct ttttttcaaa cgctccattt
      421 ttatgtggaa cattattttt ttaaggaact ttgtcaacat gtggataact attttccact
      481 tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact tgctgcagca aaaagatcgt
      541 tgcaagagag gctgttccgc agatcgctgg atctagattt ggcaaagtca agactgcatc
      601 atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc gcaaaccact
      661 gctatgttgc gatttgatac ttcgtgacaa atcaaatcca gtaacaatga ttaatggatg
      721 accattttct ttgatccact gagctagttc gatgattgcc gggtttcttc cttcattcga
      781 aagaagaaga aaaatgtctt ctgatttgac agaaagctga tcaataattg cctgtcctgc
      841 accttctagc gtttcataga cacctccggc tggattttca atcaatatac ttggtacaag
      901 atccggggtt tgatcccaaa attcaaatgc tacaccactt agtgtgcggc tagcatatat
      961 ctgcaatcta ccaccttgat taatttgttt cgcgatccaa gtgccagctt gaagcatcag
     1021 cggctcttct atccttaatt tctcttccaa ttggtgataa tagtcaaaat aacataaact
     1081 catttatctc tcccgcctag ctatacttga catgaataga aacagctgcc taatctaagg
     1141 aagtacataa aaaaacagta gttagacagt aacacatgtg gtctttgctg tttcaaacac
     1201 aaaaaaggca ttgaacgaat acgcccaata ccttgacata gcaaataaaa caacgtgcag
     1261 accatggaaa atatctacat aggggtagaa ttttcaccct agattaggta caataaatta
     1321 ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt gtgtgactgg
     1381 tcattccctg attcttagcg ccaactaagg gtcagggagc ctttttttat tcagctgttt
     1441 ctatctgaat ttattgtaag taatctctta tccaagtgca agcaagaaaa tacaaaaaaa
     1501 cattggtatg atttatatat atataaataa cataaaaaaa ggaatggctt atcccgatac
     1561 aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt tttccaccaa
     1621 ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt gcgtatacct
     1681 actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc tgtactagaa
     1741 aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat atatgggata
     1801 taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc ccaactacct
     1861 aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca gataaaaatc
     1921 agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat aagcaatagt
     1981 ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc aagcagcgac
     2041 tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata attaaaaaat
     2101 tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt atgtaatcat
     2161 tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc tgaatctatt
     2221 cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac
     2281 taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa tagcctttca
     2341 ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta ctcaactgga
     2401 taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat tcaatatttg
     2461 tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac tctctcgcat
     2521 agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg
     2581 ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga taacgtccga
     2641 taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac ccttttactc
     2701 tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta gatgtttcat
     2761 tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa tccatcatca
     2821 attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg acgacaaacg
     2881 gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct cttggatgag
     2941 attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg agtaattctg
     3001 ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag tgatcttcaa
     3061 tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa tccaatgctt
     3121 cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct tttgccatgg
     3181 cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact gcttttttga
     3241 ccttcacttg ctgttcctcc taaacgacga ttaactattg atcatctatt taacgaagag
     3301 aaaattcatt ctccattttt ccgtcacgta acatgatttc ttttgctgct tttttgatat
     3361 cttctccatt atacaaaaca ttatagattg tttgagtaat tggcatctca acacctagct
     3421 gttgagctag ctctactgct gcttttgtcg ttgccacacc ctcaacgacc attcccatgt
     3481 tttctagaac ttcttccagc ttatgtccct gtcctaacaa gttaccggca cgccaattgc
     3541 gagaatgaac acttgtacaa gtaacgatca agtcccccac accactcaaa ccgataaatg
     3601 tcagaggatt ggcacccatc gcgacaccaa gacggctgat ttctgccaat cctcgagtca
     3661 tgattgctgc ttttgcatca tctccgaatc ctaatccatg gatagcgccg gcaccaattg
     3721 caatgatatt tttcagtgct gcaccagttt ctacgccaat cacatcatta tttgtataaa
     3781 tcctgaaata atcattcatg aacagttttt gtacataggc tgctgctttt tgatcaatac
     3841 ttgcagctgt aattgttgtg atatcatgta ctgccacttc ctcagcatgg ctaggaccag
     3901 ataatacaac aattgcctgt cttttttctt ctggaatttc ttcagccaaa acttctgaga
     3961 ttcgtttatg cgtatcttgt tctagtcctt tacttgcatg tataatgatt ggcttagtat
     4021 tcattttacc aataagttcc tgtgcgactg aacgaatcgc ttttgttgga acaacaaata
     4081 aaatagcatc tgcgttatcc actgcttctg ccaattcttt atatccgata atcgattctg
     4141 ggatcgtcaa atcaggtaaa aaatgctgat ttgtatgata cttattgatt tcatcaatct
     4201 gttcaggtac atttccccaa atacggacat catgcccgtt ttctgccaat gtttgtgcta
     4261 aagcagtgcc ccaagatcca ggaccaagaa ctgcgatttt ttgtttcatg gatattcctc
     4321 caaataaata attgataatt tccattatag tatacatttc ttaaagttgg gactaatttc
     4381 cattgttttt aagtgtttgt ttacttcttt tatcgttttt acttgaaata atcaatgata
     4441 tgttacaata gtctacattt tatttttaca ctcgttatgc tttgcttgtt cctaagagga
     4501 agcagttttg acaatgttac taccagcatt actattagga ggttaggaaa cttctcctag
     4561 tgagtattga tttaaaatac ataagagaca gattgaacat ttctaaatct gtcaggtcct
     4621 gcaacggaca gattgtctct gttgacggtt gacataggaa cttttgtttt agtcaacatt
     4681 ctggatacaa aacgctatgt aaactggcgc aactcgttgt acttggactt ttactacgga
     4741 tccatgcaac aactaaatag tactattgca taacaaaaaa tggttggcac ccacatgtcc
     4801 cattaaacag attataagtt gaactgtaga taatgcaaaa aatgtgtttt ataacgtaat
     4861 tgatgccaat attttaaaaa aataaaggga gcagttatcg ctgctctctt tattttttat
     4921 attcttttta tccagtctat cctaataatc aaactgacga tagtagcggc gtatctctaa
     4981 tggatggcag ttcaactgct ccacatgagc atcgatgcgt tgtgtcacta catgcatcaa
     5041 taagaaagac aaaacacgtc cgcgatattt ttcactgata ccttctactg gatagtcttt
     5101 tgtgtctatc aatgtataat tgctgcaaat ttttggcaaa agtttagcca cacgttcaga
     5161 taacactctc tgttcatctt cgcctaaaaa taatgtgact ggggttgttt cttctacgat
     5221 ctctaacgtc ccgtgaagaa actcggcagc atggatagat ttcgatggaa gccagctttg
     5281 ttcttcccag taacacatgg catatgaata aacagctccc cattgattcc ctgctccaat
     5341 aaaataatgc atactatcac gtcgatgttt ttctgcaaat gataagccaa atgcatctgc
     5401 ttttttctct gcttccacta accctgttgg taaataacgt tcgagctgtt catagtattc
     5461 tgaataagct tcgaattctc cattcaaata catcaatcga tctgctgcca taaaaaattt
     5521 gatttgttct gttccaggag ctggataagt gaccacaaaa tcgcatagct gagacaactt
     5581 actgttcgct ttatcgataa atccaatcag tgtcgctcct actttcttga tttgttctac
     5641 tgctttcact acttcttgag ttgtaccggt aacagaggac aaaataacga tagaatcttt
     5701 tgttaatctt ttgtttccag tagtgtaata ttctgcagca tgctgtacaa agacaggcaa
     5761 gttgcttttc ccattcatat aagtgacagc ttgcatcgag gaagcatacg ttccaccgat
     5821 tcctagataa tagacagcgt caaatttttc cgcataaagt ttatcgatga tttcttctac
     5881 ttgcggacgt aaagctagtg cgccttttat tgctttgatt tgttcttctt cattgaattt
     5941 cagcatgatt tccacctctt ataatttact tttcaagact gtatcaaagt cttcaaaaat
     6001 ctttttgttc aagtcaatac ctttagctac tgcggtttta taagcgaata gttgaaacgg
     6061 aacgacaagc gctaatacag acagatattc atcacattct attgctaatc caagtgtttt
     6121 ctcgtttctt gctttttttg tggtaatggt gagagtctct ccaacgtacc gactcatata
     6181 tctttgcaac ttttgcgaac gttccttatt tacactttcc ccttcaataa agaatagcaa
     6241 atgcgaagca tctgcctcca ggtatgggcc gtgcatataa gcttctaact caaatccttg
     6301 tgacggaacg cggattgttt cagtcaattt cgtctctgct tctttagctg ttccccagtt
     6361 tggaccatat ccaatggtga cgaaacgctg tgctaaacga aataaacttt gatatccatc
     6421 aaaaaattct tccgtttttt gaataatcgc tggtaaatgg ttgattattt tgcgcaactg
     6481 ttgcatgtag tcttgttcta tttttttgct gattttattt ttacttattc caattcctat
     6541 ccctaagagt agtaactgta aaacagtcgc agaatagcct tttgtaacga agcctactga
     6601 ttctaccccg gcacctaaat caataatttg tgcagcttct aaagcaagtg gactttgcac
     6661 gtcatttgtg atagcaacaa caggtaaccc ttgtttttgt atcattttta ctgcttcaac
     6721 agtagaagca cttttcccgc tttgtgacac agcaatcaca gtatcgattg aaggagaaag
     6781 ttttccataa ttgaaaaaat aaaaaggttc ttgaatatcg acagtaagat caccataaga
     6841 ttccaatgct ggcttcgctg ctaaacaagc gttataagat gaacccgttg ctaagatcag
     6901 taaattcact gtttttttca tatgttctct tgtcgaaaaa tcattctttt tcaagatcgt
     6961 acttagtact tccttttctt catagatata atcttgcatc gtaaacattt tctctgctcc
     7021 ttttttaaaa aatccctatc cacgaaccaa aaattccaaa gaaagctagt ccgatcaaga
     7081 tcgttaatgg tttcacttct ttcttcaata gataaaacac tatgccaaat gctccaagac
     7141 ataagatatt tggaacgata tcatcaaaaa tactttgaac agtcacagca ttttctcctg
     7201 aaccgatttt taaaggaata ttgatggtga tcatcgtggc agtcattgct ccaacgacca
     7261 tcagtccaat aatagacgcg cctaatgtca aactttccat cataccgttt gcctggatct
     7321 ttttcaaaaa acccgttcct aagcgatatc cccaacctgt tgctagatag cgaaacagaa
     7381 tgtgcggaat attgaaaatc aaaatgaaca agatagggcc taaaatattt ccttgcatgg
     7441 ctaaagaggt acccacacct gtggcaatca atcgcaaagt tccccaaaag aaagaatctc
     7501 ctaaccctgc aagaggtccc atcaaagatg ttttgatact atcgatcgca gtcacatcaa
     7561 attcaggatc atccttattt tcttcctcca ttgcagccgt gattcctaaa atcagtgtaa
     7621 cgatatgtgg ggtcgtgtta aaaaactcta gatgccgttt caaagctgct gccatttctt
     7681 ctttcgactg atataacttt ttcaaaatcg gaatcattgc ataaacataa gccatattca
     7741 tctgacgttc ataattccaa gaaaactcca tttgaaaaga acgccaaaat acgtgattta
     7801 gctctttttt cgttattttg ctgtttttga tctcagaatt cttcatcttc ttctacctcc
     7861 cctgcagttg tttgaagtgg tagttgacgg acattcataa tattgactac cacaactgct
     7921 aaaatcgcac caaaaatagc tactccggtc acaggaatct ctaagtaaga agctatagca
     7981 aaacctaaga aaaaatatgg tgcaacttgt ttgttgatca gcaaacgagc aagcatcgca
     8041 aaaccaagag ctggaatcaa ccctgtagct actgacaacc ccttttgaac gaagtcagga
     8101 atcatattta atagatttcc gatcgtattg cttcccacca tataagaaac cattaccaca
     8161 acaccaagca tcaaggaaag accgataccg gcaagcaagt gcatatgctc gactccttta
     8221 taatttcctt cttctgcata attgtctgct ttatggttca taattggaat caaaagccct
     8281 aagtaaatat ttttcaatat caatgtcaac gtagcaatcg gcaaacctag taaaagcgcg
     8341 gtttctgtac cggctccagc tgtaatagca aaagccgtcc ctaaaatccc accggtcaca
     8401 acatcaggtg gaatcgaagc acctactgag aacgatccga taaatgctaa ctctaaagtt
     8461 gcgcccataa tgatcccagt ttttacatca cctaacacga ttcctgtaaa cagccccgtc
     8521 acaatcggac gagaaagcaa cgatgtccct agtgcatatt ctgattgtgc gacaaatgca
     8581 acaattccta ataagactgc ttgtacaatc attgaggttc ttcctttctt acaaaacttc
     8641 tcttacatcc acttttttat ctgctggtac ttgacggatt tctacttcta tttgtttgtc
     8701 aattaatcgt ttcaagcgtt cctcctcttc tggtaaaata tttaccgctt ttccgatgct
     8761 gcgagtacca ggttttgcct tgatccctcc taagttgatg gttttgattt ctggatacgc
     8821 ttctgccagc ttatatgcat cctcgatcga ttccactacg atgaataact tatatttatc
     8881 tgtcaccccg cttttcaacg cggcaatcga gtcatcaatt gatttgatca ctagtttgac
     8941 tccttgtggc ttggctaatt tgatcgttgt ctttcgtatc tcattcgtag gtacatcatc
     9001 attagcaatc aaaatacagt ctgcgcctat tgtttgtgtc catgaaaaag ctacttgtcc
     9061 gtgtaataac cgatggtcga ctcttgtcaa tacaatcatt tttcttcctc ctaaaattcg
     9121 tcttcttcta ttttctgatt gagccgctcg ttacaaaact ggatcatttt ttttgaatca
     9181 gttagcacct gctggatcaa aacagttgtc tctacttcag cgtccaaccg agatactagc
     9241 tcaatcaata gtggcaaatt caatccggca acgagataga catgatcccg attgatatat
     9301 tgtaagaatt cgttgttgac acttccgcca aaaagatccg tcagaacaat gatttcttta
     9361 tctttgtacc tcataaaaag ctgccttact gtttccgcta aatcaaaatt ctcatcagta
     9421 tagcaatcga atacagtaat agaatgattt ttcccataga tcagctctaa tgaatctaat
     9481 atccctgaag caaattttcc atgggaagct aagataattt ctctatccac tttttcatct
     9541 cctcttttca cttacacata ctaaaaaagc aagttgcatg ccaaaattag tggcaacaac
     9601 ttgcttatgt tcaaaattct tcttctatcg agagaaactc tgtatcttca ctcaaaatat
     9661 catagatata ggcaatctca gaattgggga tcatgacact ataatgctct tcaatgacac
     9721 taaacgcaga tttaatagca gataaccctt ctttttgaca ctgcttccat ttctctaatc
     9781 cttgataggt ttcgattggc atttgtcgga tcaatcgttc aatgagacag ctcacatgga
     9841 catacaacgc taattttttg ggattgctga tgcttcgatt caactgcacc tctaattctc
     9901 tcataaaatg atcgatttct cccatcacct tctctgtatc tagaatagtc acggaatcca
     9961 gtactttttc caatgaaaaa ttacgaatga tgcgctgatt gaatatttcc ttctctgaag
    10021 cattcatagt agtgtccaac cattcagcta gtacagacat cttttccccc gagatgaggt
    10081 cttctaatga aagatagtcg atattggtga tatcaggatc tgtggtgcca atcattccta
    10141 cgacttcgta tatggaaaat aatgggtcac tgtttttctt atcttttaat tgctgatatt
    10201 catatgggat gattttcaac tgacaagccg taggtaagct tttttctagc agttcactta
    10261 agtggaccgc cgtaccgatt ccagtgaagc aagtagtaat gagtgctttg ggtttgtttt
    10321 cctctggata gagcagtcgc cattctggct ggatctgtcg aacagtctct tctgctatct
    10381 cttctaatga tacatttttt tgcaggcatt cccctactgt gattgctaaa ggtgtcgtca
    10441 cattattcaa aatcaaaagc ggtacggata actgtttttt aaaaaactga tggatttctt
    10501 tcagagatcc catgtctact aatatgatca atccatttga aacatcgctg ttttcgcaat
    10561 aatcaaggat ttctgaagca atctgctgtg gtgtgacatc tagtggcata tcaaatgatt
    10621 caaatagatc ctcttgtaat aaacgattta ctacatttgc catactgcta gcagttgcat
    10681 aaccgtgagc agcaatcatc gcttttggca gtcgtttttc ttttgaccat ttcgcttttt
    10741 ttagatagat cgtcagtaaa atccgatcca tacttgtgtc ttcaatatct aatttgatct
    10801 tacttaattc cagaattcgt tggacatagt gataactaga tggataagac tgagaaactt
    10861 gttgttctaa ttctttgatt agttggataa tatcatgttc ttccggtaac catttgatag
    10921 tgcttcgctg atagagatag taactcaagg catagacact gtttccatcg aatttgattt
    10981 gataggcagt ttccatttgt cgaaaggtct ctcggataga ttgtgtccaa taaaccagca
    11041 gctcgtgttt ttcttgtcta ctggtctcaa acagcaaaaa atcaaataat tgaatcactt
    11101 cttgctttaa ttttccttcg caagaatata gcagacagtt atttttcttg aaatcaatta
    11161 ggatttgctc aaaactttta acgattcgct gctgttcggg ctgttgcttc tccattaatt
    11221 gctctaaggt ctggtcttgc gtaatataga ttccttgctg tgctgtattt ttagtaatag
    11281 gctgcgcaag tagttctttt tgcaaatgat ggatcgtgac atggatttca ggctgttcac
    11341 gctgttcagc atatgctttt gctgctgaaa ctttgacgat atttttcagc tctcctatgt
    11401 ttccggataa tttctgactt gttaaaagtg acaaagctgg tccactgact gacaaagggc
    11461 tccccatttt tctttgttct tcgagaaaaa aagagtaaat cagttctagc cgttcattac
    11521 gactacgctg attaagtgca ggtagttcga ttcggatagg tattcttcga atgaaagttg
    11581 ttaagaaatg actagtaatc tcttcagtcg tagcaaaaaa gagacgtgtt ttgatccggc
    11641 gtgacaaatt aggttctccc atacgataaa tcacaccttg atcaagatag gtgaacagtt
    11701 tttcctgacc ttctggtgtt aagcgatgta cctcgtctaa aaacaagatc ccaccatctg
    11761 ctgcttcaaa agcccctttt ttattttctt ctgcaccagt gtacgctccc ttaacatgtc
    11821 caaacaaatt gctagtcaaa agttcaggat tatctgcata ttgtgcacaa ttaaaagtga
    11881 tgaatggtgc agaatcttct agcagatcat gaagcagaca atactgatag accaaattaa
    11941 ctaaataact tttacctgtg ccactttcgc ctgtaatcaa aagtggtaat ccgccatctg
    12001 gatagtaaag agctgttttg atttgttcga tactttcttt caaactggcg tcgtatccga
    12061 tcataaaaga gaataagtct tttggttgcg cgaaaaaagg ctgttcctct ttgagatcat
    12121 cgatagaaaa ataaaaattc gtgcgtaact taaaaaattg tttttcaaaa gcttctttgt
    12181 ggaaataaat cactggtcga gaattgattt tgactagtag ttgctcatca tttaactggt
    12241 tcaaataatg actgactgtg tttcttttta ccccaaaatg atcagctaac ctttgtgctg
    12301 taaagacctc gttcatatgt tctaaatcga aaaaggctgt ctggttctct aagtaacata
    12361 aaatttcctt cttcaacaac ttattcaccc ctcctccatc atagaaaacg gattcatttt
    12421 gtcaataaaa tctttaataa agaattgaaa aatatatctt attatagaca gcagcctact
    12481 tatcccttat aatttttatt ctaaaaaacg tacagttcct tccagttttt atactggaat
    12541 ttcaacatat gaaagaataa ggaggctatg actattcaat gaaatcggat aaatataaaa
    12601 acaggaaaca tacttcctgg aaaacttttc gagaaatcat cggatcttat ctcgctgtag
    12661 ttgtattttt gcctttgctt tatatggcta tgacagaaca aactcgtttt attggcgtag
    12721 atacgcaata tatagccgaa acaggatttt tattcatctt tatcttgatt tttgcgttct
    12781 ataaatcctt ccatacaaaa taagattcac agatttcaac tttcctgtct gtttttagct
    12841 attacaaatc cttatatgtg gtataacaac aatttattgg cataatgagg aaaaacgagt
    12901 tatgatgctt tcagagagta aagaggtact gttgtcacca tcctttttat tgattacgta
    12961 caccactctt agttttttcc cattgattac gaaaacaagg tagaaacatt gatgttccta
    13021 ccttgttttc tttttttatt ttcagatcaa aatctcaagt agcttttgac ttgatgtcgg
    13081 gaggttgtct tcgttccatc aaacctgata gaatctgcgc caaaccatag ttttctccac
    13141 tattatttat tcctctaaga tagtacccac aataaacgaa taaatatttt tctcattttc
    13201 atactccttc tttgaaaatc aattagattt ctctcaaccc acttttttac aaaaattacg
    13261 tagcaaaaaa gattgcaaga acaaattaaa aaagaagtaa atagag
//
LOCUS       DAZKCC010000082        13210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000082 DAZKCC010000000
VERSION     DAZKCC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.084"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..1916
                     /gene="yfcC"
                     /locus_tag="ACG8VH_002362"
     CDS             177..1916
                     /gene="yfcC"
                     /locus_tag="ACG8VH_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9110898.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2073..3257
                     /gene="iadA"
                     /locus_tag="ACG8VH_002363"
     CDS             2073..3257
                     /gene="iadA"
                     /locus_tag="ACG8VH_002363"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9110899.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3368..3571
                     /locus_tag="ACG8VH_002364"
     CDS             3368..3571
                     /locus_tag="ACG8VH_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9110900.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3672..4334)
                     /locus_tag="ACG8VH_002365"
     CDS             complement(3672..4334)
                     /locus_tag="ACG8VH_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110901.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4347..5849)
                     /locus_tag="ACG8VH_002366"
     CDS             complement(4347..5849)
                     /locus_tag="ACG8VH_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9110902.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6397..8076
                     /locus_tag="ACG8VH_002367"
     CDS             6397..8076
                     /locus_tag="ACG8VH_002367"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9110903.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8203..8682
                     /locus_tag="ACG8VH_002368"
     CDS             8203..8682
                     /locus_tag="ACG8VH_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP9110904.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8901..9581
                     /locus_tag="ACG8VH_002369"
     CDS             8901..9581
                     /locus_tag="ACG8VH_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9110905.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9589..10920
                     /locus_tag="ACG8VH_002370"
     CDS             9589..10920
                     /locus_tag="ACG8VH_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP9110906.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10935..11897
                     /locus_tag="ACG8VH_002371"
     CDS             10935..11897
                     /locus_tag="ACG8VH_002371"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP9110907.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12008..12346
                     /locus_tag="ACG8VH_002372"
     CDS             12008..12346
                     /locus_tag="ACG8VH_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9110908.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12425..13132
                     /locus_tag="ACG8VH_002373"
     CDS             12425..13132
                     /locus_tag="ACG8VH_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP9110909.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4217 a         2324 c         2891 g         3778 t
ORIGIN      
        1 ttaatataaa tggaaagaga aaaaaataat catacatagg agaaaaaaaa caaaatagaa
       61 aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca tttttactgt ataattcatt
      121 tgattatcca ttttttattc atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga
      181 agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac taaattatcg acaccccata
      241 cgtatgtcat tatttttggt gtagtcatct ttgcatggat actgactttt gtcgttccag
      301 caggaaagtt cagcacgcaa gatattgaat acaaagatgc aaatggcgaa acaagtactc
      361 gtactgtttt acgtcaagat tccttccgtt atgcttacga gctggataag tcgtatgtgt
      421 ttgatcaatt agaagaactg caagatcatc cagcagaacg tgaaaaactg gatgttccgg
      481 aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag gaaaaactag
      541 atgaaatctc cttgacagat gatgtgcttt acgatcagta cggcgaaaat atctatgata
      601 cttctaaaaa attgcataaa acagccaaaa tatggggaac agatgatttt ggcggctttg
      661 gtttcttaaa ttttgttttt gaaggactag tatctggaga taagtatgga tctgctgtag
      721 ggatcgctgc attgatcctt gttgttggag gtgcttttgg tattattatg cgtaccggag
      781 cgatcgacgc aggtatctat gcgtttatca gtaaaactaa gggactggag cgtttagctt
      841 taccgctatt attctttgct ttttcattcg gtggcgctac attcgggatg gcagaagagg
      901 taattccatt ctcaatggtg atggttccat ttgtcattgc gttaggatat gactcgatcg
      961 tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc
     1021 cgtttagcgt agcggtggct caagggattg ctggaatccc cgtgttgtct ggtgcgacat
     1081 tccgattgat tatgtgggta gttgttactg cattagccgc tggttatatg atgatatatg
     1141 cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat gattatttcc
     1201 gttctcatat caaaaagaca tctgatgaaa ataaaaaatt catgcttggg cataaattga
     1261 tcttattaga gatgttagtt gtccttgtct ggatcgtttg gggcgtgact caaaaaggct
     1321 attacattcc agaaatcgct tctcaattct ttgtgatggg attagttgct ggaatcattg
     1381 ctgttctttt caaattagat ggaatgaaag tcaatgacat cgcttcttct ttccaatctg
     1441 gtgcagctga tttagctggt acagcaatcg tagtaggtat ggccaaaggg attcttcttg
     1501 tcttgggagg atctgatgca tctgttcctt cagctttgaa tacgattttg catgggatcg
     1561 ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa
     1621 gtttgttcaa cttggttgtt acatcaaatt caggacaagc agctttgact atgccaatca
     1681 tggctccact ggcagattta gtaggtgtat ccagacaagt agcagttctt gcttatcagc
     1741 ttggttcagg ttttatggac gcttttacac ccgtatcagc cagcttgatt ggtgtgctag
     1801 gtgttgcacg gatcgaatgg gcaaaatggg caagattcca aatcaagatg caaggattcc
     1861 tgtttgtttt aggaactatt ttcatcatga ttgccattgc aataggtctg cagtagtact
     1921 ggagacctgt aaataaaaaa gtaatccaaa cagtagataa ggactccaag aaccgtcatt
     1981 ttacggtaaa atcaaaggag taaagatcct atcgtttgga ttatttttta agatcctatc
     2041 atcagttata cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt
     2101 ttgcaccaga ttacttagga aaaatggatg tactgattgc cggtgggaag attttggctg
     2161 tagaagaaca gctagaaggc ggctacgaag gagtggaagt agaagaactt tcaggtgagg
     2221 gaaaagtact aaccccgggt ttcattgact gtcatttcca tattttaggt ggaggaggag
     2281 aaggcggtta tcaaaatcga acgccagaag tcacattgag ccaattgact acagctggtg
     2341 taacgacagt tgtcggttgt ttgggaacag acggagaagg tagagatatg actgcgttga
     2401 tcagcaaagc gaaaggtctg gaagcagaag gaatctccac ttatgtatac gaaggttcgt
     2461 accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa agactttttg acaattgata
     2521 aaattatcgg aatcggagaa atcgcagtat ccgatcaccg ttcttctcag ccaagttttg
     2581 aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg catgttatcc ggaaaagctg
     2641 ggatcatcaa tgtccactta ggcggaggaa aaagaaagct agagctattg acacgaatcg
     2701 tagaagaaac agaaattccg atcacacaat ttttgcctac tcatgcaaat cgcacaccag
     2761 aattatttga agcttgcgtg gcatttgcca aacgtggtgg cacgattgac ttcactgcta
     2821 gcgaagatcc tgatttttgg gagaagacag atggtgaagt tcgcttcagc aaagcactga
     2881 aacgattgat cgaggaagat gtttctttag ataatttcac gatgacctca gatggacaag
     2941 gaagcttgcc ttattttgat gaaaacaatc atttcctagg gttaggagta ggaagtgcca
     3001 aggcattgct agtagggatc aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg
     3061 ccttacgagc tataaccagt aatccagcac gtatattgaa attggataaa aaaggaaaaa
     3121 tcgaaattgg tgctgatgca gatctgtgta tcttggataa agaaacgctt gatattgata
     3181 cagttatcgc taaaggagaa ataatggtcc aagaaaaaga agtcaaagta tgggggactt
     3241 tcgaaaaaag tttttaatca acagctctaa ctatcttttt tagaaaacga gcgttctgtt
     3301 tgtctgtatc tgttgttgat gttaccctga aggtagatca ataaacaaac taagaggagg
     3361 aagcacaatg gcagatttaa aaggacgttc taaagatatg aaagataaag tagtcggtga
     3421 aggaaaagaa gcatacggca aagcaacaga cgacaagagc aaagaaacag aagggaaagc
     3481 acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga
     3541 caagttttct cgaaaagacg aaaaaaaata aaaaccactt attactaatt gaatcaagca
     3601 aataaaaaat ccgaaccata tctgcgagac agatatggtt cggatttttt atttgatcat
     3661 tatcctgttt tttaaacttc gattttttga aattttttat tttttagttc aaagacttca
     3721 tcacagcttt ttgctacttc aatctcatga gtcacgatga taatacagcg attttgttca
     3781 tgggcgattt cttggaaaag tttgacgata tcttgtgaag ttgtttcatc tagatttcca
     3841 gtaggctcat ccgctacgat tagttgatgt tcacagcaga tagcacgaac gatagcgact
     3901 cgctgctgct gacctcccga aagtttggtc acctgttttt ttgctaatat ttcatcaatt
     3961 cctacacgag ccagtcgatc taaagcaact tgtcgataat tctcttgctt ggtcttagtg
     4021 atttccattg ctgctatgac gttgtctaga gccgataaat aaggaagcaa gttgtaagct
     4081 tggaaaataa tcgagacatc atttttacga taacttctta acccgatttt gttcagtgaa
     4141 cgatcttgat agtatacctt tccttctttt ggtgcatcta gtccagaaat aagcgaaaga
     4201 aaagtcgttt ttccagaacc acttgctcct aaaatagcgt acatttttcc agcttcgaaa
     4261 ttcagttcta catctttata aagcgcatct tgcggatctt gataccaaaa tcccaattgt
     4321 tctgtccgta acatcaaatt cctcctttag tttaatagga tttttcttgg gtttaatctt
     4381 aatatcccta tagaagctaa aaagacagaa aacagactga ttccaaatgc tactccggct
     4441 aatagtccca attgtgcagg tttgacattg atttccaatt cgctgacttg atcagaaaca
     4501 gcaaatggat tattcgtggt tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg
     4561 ttacctggca tttgtccacc gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt
     4621 tggtcaagta gctgctgtcc cacggcattc gcgacgacgt ttccgctaag cgaagcaact
     4681 cctaatgcaa gaatcataca aatagccact tcggtaaaca attggagaat gattttactt
     4741 ctggattctc ctaaagatag caagacgcct aactcatgcc gacgctcccg aatggtaatc
     4801 ataatgatca gagtaagtat gataataccg gctacagcca ctagtaaaac aacattttta
     4861 gaaaaacttg ccacgttgtt taaaggttcc aacattgact gatacatgga atcatttgat
     4921 tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt
     4981 ttatctggat cagatagagt gtaaatagca gaatctatcg tgttatcgtc agatgtccca
     5041 tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt catgaagttg
     5101 aatttcatac ccatactgcc gcttgtttct ctggattcat aaattccttt gatcgtcatt
     5161 tcataagtgg tatcttcatc ttcagtactc gtgattgtga aagtatctcc gactgccagg
     5221 tcatttgttt ctgctagagt agaatcaatg actacattgt ttgtgccttt gtcgctgtca
     5281 tctattcctt caccatccgt gatttttgct gttccatcag aaaaattact attttgtgca
     5341 gttgctgaca caccactgat ttgaaagtct gcttgagtca tttgcggatt cccgcccatt
     5401 cctccagcag gttcaccgcc tgcatcagta gtttctgaac tgtcttcact tgaatcagaa
     5461 ctagtgatgg cagttattcc atcttttgcc atagctgagg cggaactttc aaaggaataa
     5521 ctctttacat tatctaattc agctattttt tccgcgtccg ttaaggaaac tggtgtaaga
     5581 gaaaaactgc ctggatctgg acgttcttca gtggtagaac cgcttgaaga agtttgttcc
     5641 tgttttttaa atgaagcttc tcgattggca cttaaagtca ctgttgctcc aacttctttt
     5701 tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa
     5761 ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac gtccccattt tacttttgta
     5821 cttttccatg cacgtttcat aaaattcata ttttgcatcc ctcctagtag atgcatttga
     5881 taatagacaa cctatcttaa agctagctta aatcttatgc tgtttgcaca ccatcaacat
     5941 gaggttctgt atatataaaa agtcactata gttattgcaa aaaatgtggt aaaatgaaaa
     6001 aagtatgttt caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga aatcctagat
     6061 gctacagagg gatttttacg tgcatagttg ttctaacaat tgctgatact agcgatttag
     6121 tttatttgtt gtataataat ggggaacagc tgaatactta tataagtatt ctggctatag
     6181 cgtttcgcaa aagatccatt ttgttaatga agtatatcca aaataaaatg cccatccaac
     6241 tgtcagaacg cggatgtgct ggtaattttc ctgctgattc tccataagtt gagaaatagt
     6301 gattttgcat atgcttcatt ggaatggctc atatatttag ataaagaaga aaatcgacat
     6361 ctttaaactt ggatgtcata agaagaggtg aagtttgtga gtaccattaa aattatccca
     6421 ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt
     6481 tttattttag attgtgggct aaaatatcct gaaaatgagt tgttggggat cgacgtcgta
     6541 atccctgatt ttacgtattt agaagaaaac attgatcgtg tagctggtgt ctttttaaca
     6601 catggacatg cagatgcgat cggggcactt ccttacttgt tgtcaaaaat ttctgtacca
     6661 gtattcggga cagagttgac gatcgagtta gcaaaattga atgtcagtcg taatgaagca
     6721 gccaaaggat ttaaggattt ccatgtggta gatgagcata cagaaataga ctttggtgaa
     6781 acagtagtga gctttttccg taccactcat acgataccag attcagtggg gatcaatctg
     6841 aaaacaaaag aaggtagtat cgtttatacg ggagatttta aatttgacca aacggctatc
     6901 ccaatgtatc aaacagatta tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc
     6961 ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg tagcttctga agcccaaata
     7021 gcagatgaag tatttgatac gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta
     7081 gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa
     7141 gttgtcttga ctggtcaaga tttcgaacga attattcgca cagcgatgcg tttagaaaaa
     7201 cttcaattac caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca
     7261 gaagaactga tcattttaga aactggccga atgggtgaac ccatcaagtc tctgcaaaaa
     7321 atggcaaacg gtacacaccg taatgtcaag attgaagaag gagatttggt ttatatcact
     7381 actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat catttatcga
     7441 gctggtggaa cagtgaagtt gatctctgag aatatgcgcg tttctggcca tgccaatcct
     7501 aatgacttgc aattaatgat caatttgatg aaaccaacat attttgtgcc agttcaagga
     7561 gagtatcgtg aattagctgc tcatgcagat ttggctcatg cagtagggat gccatacaaa
     7621 aatatctata ttactggacg cggagacgtg ctggaatata agaacaagcg tatgagtgtt
     7681 gcaggaacta caagtgcaga aaatgtaatg attgatggat taggcatagg tgatatcgga
     7741 aacattgttt tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt agcggttgtc
     7801 actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc acggggattc
     7861 gtttacgtaa aagctagccg tgatctgatg aaagaaagca gcaatatgat tgaaacaatc
     7921 gttgagaaac atttagaaag caatgatttt gagtggagca aattaaagca agacatccga
     7981 gatcaattag gccgttactt atttgaacaa acaaaacgcc gcccagtgat cttgccagtc
     8041 attatggaag ctactcaacg tcgaaataaa aaataaacaa aaagagtaag atgaacctct
     8101 ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg aatacagcat
     8161 ccctcgcttt ataataaacg atagaataaa gggagtgaat caatgatgag tcaaacaaaa
     8221 gtcgaacgtt atttagaaga acaaaagatc agctatcaac cacttgattt tagtcatgtg
     8281 gtagaagaat ctttcacaga agaactacaa aaaagaggga ttgatccagc tttgatatgt
     8341 aaaactctcg tagtgaaagg aaacaaaact ggggtgatca tcgcattgat tcctttgaac
     8401 gatcacttag attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt tggttttcct
     8461 ggaatggatt ttgtattaat gcatacagga tatccccacg gagcaaacac ccctattgga
     8521 ataaaactcg cacatcctga ttattgtttt ttagcagaca gttctttaaa agaaaaagaa
     8581 gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt agccgattta
     8641 gaaaagatcg tccatcctat ttacgctgat ttaattagtt aagggacgag aagagaagct
     8701 aagcagagca gagacctcaa acttgagatt atatctgctt ggtacttggt gcatcctgta
     8761 ttatctgtta caattgcttt gtacatactg tactatcaat ccaatgaaaa tatatttagc
     8821 agctatattt tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga
     8881 gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt ttaacaattg
     8941 aaatggaaaa tggccaacgc gtgatgattg acccgtttat tacaggaaat ccattgacag
     9001 acttaactgc agaagaagta aagacagatt ggttgctgat tactcatggt catagtgacc
     9061 acataggaga tatgattcct attgcaaaag aaaatcaagc cacaattatc agtattgttg
     9121 agattgctga ttatgcgaac agcagaggag tagattcatt cggtatgaat atcggtggaa
     9181 aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc acagcatagt tcaagctatg
     9241 aagtagacgg tatcgttcag tatatgggcg agcctagcgg aattattatt caagcagaag
     9301 gaaaaacgat ttatcatgca ggggatactg cttatttcag tgatctagga ttgcttgcag
     9361 aagaattcga tattgatgtc gctttcttgc cgattggcga taactatacg atggggccag
     9421 aagatgcagc aaaggctgcg caaacaattc gtgccaagaa agtagttcca atccattaca
     9481 atactttccc tgtcattgaa caagatcccg aatcttttgt agagttactg gaagatagtg
     9541 aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa
     9601 catgatttga ttttagaata tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga
     9661 agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca
     9721 gaaaatgtcg gactagtttc aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa
     9781 ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg tcaggatcat tgaaggggac
     9841 gttttaggcg gggcggcagg attagacaag gtcttgaata aattcgtcat cggcgcgatg
     9901 actaaacatg ccatggaacg ctatattacg ccaggatcac taatgatcgt cggcaaccgg
     9961 gcggaagtcc aaaaattagc attggaagat ggcgccgcag tattgatcac aggaggattc
    10021 aatacgactg ctgagattgc aaagctagct gatgatcttg aaatgccgat cttacgcacc
    10081 tcttatgata catttacagt cgcaaccatg atcaaccgag cgttaagcga ccaattgatc
    10141 aaaaaagata tcatgcttgt cagtgatatc tatatgccag ctgaaaagac tcattatttg
    10201 cagcagacag atacgatttc tgattatcag cgtttgtcag aagaaacgca gcattcgcgt
    10261 tttccagtcg tcaaccgcca tcatcgtttg atggggattg ttacggcaaa agatgtttta
    10321 gggaaatcgc caaaccagat catcgatcgg gtgatgacga aagaaccaat cagtgtaaag
    10381 aaaacaatga gtattgcctc tgttagccat caaatgatct gggatgggct agaagtcatg
    10441 ccggtcgttt ccgatgactt gacattggaa ggcttgatta cccggcaaga tgtaatgaaa
    10501 gcgatgcagc ttgtccagcg acaaccgcag attgcagata cgatttcgga tcagatatct
    10561 ggaaatatcc atacgattga tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc
    10621 aaattcgtag tagcaccaca aatggtcaat ggcgttggaa ccatttcttt tggcgtactt
    10681 agcgaaatca tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc
    10741 ttgattgaac aagtgaacct acattatttg cggctgatcc aattggaaag cgaattagac
    10801 attcgtccac gtgtattgga aatcggcaga cgttccgcta aactggatat cgaagtgttt
    10861 attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga acgttcttag
    10921 aaagcaggag tgaaatgaca gtacagcaag aaatagtaga tacaataaaa aaatacgata
    10981 aaatcattat ccacaggcat ttgcgccctg atcctgatgc attaggatca caagtaggat
    11041 tagccgaact attacgggaa agtttcccag aaaaagaaat acgtcaagta ggtgaaacga
    11101 ttgaagggct tcgcttttta gcagaaatgc aagaagttga agatgtcttc taccaaggag
    11161 cgctagtcat cgtgactgat acagctaatt cacccagaat cagtgatcaa agatatcagt
    11221 tggcagaaaa actgatcaaa atcgaccatc atcccaatga tgacccttat ggagatcttg
    11281 tgtgggtaaa tacaaaagca agtagctgta gtgagatgat tgcagaattt gctttgatgt
    11341 ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt actttatgca ggaatcgtcg
    11401 gagatacagg gcgctttttg tatccctcca cgacgtcccg tacattagag attgcttcga
    11461 tattgagaaa ttatccattt gatgcaagtg ctttgaacag agagattgaa caaatgccga
    11521 tgaaagtcgc taaactttcc ggttatcttt atcaaaatat ccaggtggat gaaaatggtg
    11581 caggaagaat aatccttccg caagaaattc ttgataaata tggaatcgat gattcagaga
    11641 cagcttcgat cgtttcactt cctggagtga ttgacgaagt gctggcttgg gggatttttg
    11701 ttcagcagcc ttcagggtat taccgcgtac gtttacgttc taaggggccg atcatcaatg
    11761 aattggctaa acgccatcat ggaggaggcc atccattagc aagtggtgcg aatgcaaaag
    11821 atttagcaga agtagaggaa atctataaag agatccaaga tatatgccaa tcgtataaga
    11881 gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat
    11941 gtatcttttc aagatcttct ttttccgtta caatagtgct cactaaagat tttaaaggag
    12001 aaagaagatg acattaccaa attgcccaga atgtggctca gagtacacat atgaagatcg
    12061 aggcttgttg atttgcccag aatgcgggaa cgaatggaac aaagaagaac aagaaactgt
    12121 catagaagac ggattagtgg tattagatgc aaatggaaac cgtctgttag agggcgatag
    12181 cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac
    12241 aaaagtcaag aacatccgtt tagtagaagg cgatcataat atcgattgca aggtagaagg
    12301 atttggaccg atgaaactga aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt
    12361 cttttcacat tcttttccta ctactaatga taaaataaag taaacgaaaa ggagtgaaac
    12421 gaaaatgtat ggttatggaa tgtatggttt tttagatccg acatttcttt tggtcattct
    12481 tggattagtt atttccggaa ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga
    12541 tcaagtgaga agcaaaaaca atgtgactgg tacacaagca gcacaatata tattacaaag
    12601 tcaacagata aataatgtcg gtgttcaaca aattgccgga gatctcacag ataactacaa
    12661 ttcaggtaat aagatgttga gtttgtctca agcaactgca cagtctactt cagttgctgc
    12721 gataggtgta gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc
    12781 attgaagatt cgtgcagcac ttgttccagc agctaacttt ggttcgatga tttcgttccc
    12841 cttgattatg gtaggtgtat tgcttagctg gaactctacg ttaatcaata tcgggatttt
    12901 cgctttttct ttagcattac ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc
    12961 tcggcgagca atccaaattt tgagtgaagg cggactactg acggaagaag aagtaccgat
    13021 ggcaagacac gtattatttg cagcagcact gacttatgtt gcagcagcgc tttcgacatt
    13081 cctgcagctg ttgcgcttga tcttattgtt cggcggcaac cgaagagatt aaaataaaga
    13141 agttgtgaaa tcagcgcttt gatctgagct aataccgaga gttagctgat tgaataccat
    13201 ttatttggaa
//
LOCUS       DAZKCC010000083        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000083 DAZKCC010000000
VERSION     DAZKCC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.085"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8VH_002374"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8VH_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9110910.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8VH_002375"
     CDS             complement(323..892)
                     /locus_tag="ACG8VH_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP9110911.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8VH_002376"
     CDS             1078..1980
                     /locus_tag="ACG8VH_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9110912.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8VH_002377"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8VH_002377"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9110913.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8VH_002378"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8VH_002378"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9110914.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8VH_002379"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8VH_002379"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9110915.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8VH_002380"
     CDS             8554..8739
                     /locus_tag="ACG8VH_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP9110916.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8VH_002381"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8VH_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9110917.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8VH_002382"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8VH_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9110918.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8VH_002383"
     CDS             11896..12705
                     /locus_tag="ACG8VH_002383"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9110919.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKCC010000084        12492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000084 DAZKCC010000000
VERSION     DAZKCC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.086"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8VH_002384"
     CDS             <1..375
                     /locus_tag="ACG8VH_002384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110920.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8VH_002385"
     CDS             complement(324..893)
                     /locus_tag="ACG8VH_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9110921.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG8VH_002386"
     CDS             complement(967..2256)
                     /locus_tag="ACG8VH_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110922.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG8VH_002387"
     CDS             2514..3458
                     /locus_tag="ACG8VH_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9110923.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG8VH_002388"
     CDS             3472..4545
                     /locus_tag="ACG8VH_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP9110924.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG8VH_002389"
     CDS             4535..6706
                     /locus_tag="ACG8VH_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFP9110925.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8VH_002390"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8VH_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP9110926.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG8VH_002391"
     CDS             complement(9009..11195)
                     /locus_tag="ACG8VH_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9110927.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8VH_002392"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8VH_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP9110928.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG8VH_002393"
     CDS             complement(11417..11857)
                     /locus_tag="ACG8VH_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP9110929.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG8VH_002394"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG8VH_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aa
//
LOCUS       DAZKCC010000085        12259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000085 DAZKCC010000000
VERSION     DAZKCC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.087"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..649)
                     /gene="frr"
                     /locus_tag="ACG8VH_002395"
     CDS             complement(92..649)
                     /gene="frr"
                     /locus_tag="ACG8VH_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9110930.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACG8VH_002396"
     CDS             complement(652..1374)
                     /gene="pyrH"
                     /locus_tag="ACG8VH_002396"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9110931.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACG8VH_002397"
     CDS             complement(1510..2391)
                     /gene="tsf"
                     /locus_tag="ACG8VH_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9110932.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACG8VH_002398"
     CDS             complement(2490..3272)
                     /gene="rpsB"
                     /locus_tag="ACG8VH_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9110933.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3631..4110
                     /locus_tag="ACG8VH_002399"
     CDS             3631..4110
                     /locus_tag="ACG8VH_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9110934.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4327..5418
                     /gene="phnW"
                     /locus_tag="ACG8VH_002400"
     CDS             4327..5418
                     /gene="phnW"
                     /locus_tag="ACG8VH_002400"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP9110935.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5411..5539
                     /locus_tag="ACG8VH_002401"
     CDS             5411..5539
                     /locus_tag="ACG8VH_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP9110936.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5543..6088
                     /locus_tag="ACG8VH_002402"
     CDS             5543..6088
                     /locus_tag="ACG8VH_002402"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP9110937.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACG8VH_002403"
     CDS             complement(6542..8233)
                     /gene="argS"
                     /locus_tag="ACG8VH_002403"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9110938.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACG8VH_002404"
     CDS             complement(8653..9600)
                     /gene="arcC"
                     /locus_tag="ACG8VH_002404"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9110939.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACG8VH_002405"
     CDS             complement(9715..10734)
                     /gene="argF"
                     /locus_tag="ACG8VH_002405"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9110940.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACG8VH_002406"
     CDS             complement(10825..12054)
                     /gene="arcA"
                     /locus_tag="ACG8VH_002406"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP9110941.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3364 a         2535 c         2205 g         4155 t
ORIGIN      
        1 taattttttc tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg
       61 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
      121 cagcagcgat cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt
      181 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      241 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca
      301 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      361 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      421 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      481 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      541 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      601 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      661 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      721 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      781 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      841 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      901 gataacatct gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta
      961 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
     1021 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1081 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1141 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1201 ccagatattt ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1261 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc
     1321 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1381 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1441 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1501 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1561 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1621 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1681 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1741 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1801 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1861 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1921 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1981 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     2041 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2101 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2161 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2221 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2281 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2341 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2401 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2461 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2521 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2581 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2641 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2701 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2761 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2821 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2881 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2941 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     3001 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3061 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3121 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3181 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3241 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3301 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3361 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3421 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3481 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3541 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3601 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3661 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3721 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3781 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3841 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3901 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3961 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     4021 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4081 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4141 tgttttttta ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata
     4201 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
     4261 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4321 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
     4381 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4441 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4501 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4561 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
     4621 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4681 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
     4741 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
     4801 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
     4861 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4921 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
     4981 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     5041 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5101 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
     5161 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5221 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5281 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5341 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5401 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5461 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa cccaactgac
     5521 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5581 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5641 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5701 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5761 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5821 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     5881 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5941 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     6001 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6061 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     6121 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6181 aagaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     6241 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6301 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     6361 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     6421 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6481 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6541 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6601 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6661 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6721 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6781 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6841 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6901 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6961 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     7021 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7081 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7141 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     7201 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7261 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7321 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7381 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7441 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7501 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7561 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7621 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     7681 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7741 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7801 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7861 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     7921 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7981 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     8041 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8101 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     8161 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8221 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     8281 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8341 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8401 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8461 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8521 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca
     8581 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     8641 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     8701 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     8761 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     8821 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     8881 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     8941 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     9001 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     9061 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     9121 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     9181 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     9241 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     9301 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     9361 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     9421 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     9481 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     9541 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     9601 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     9661 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     9721 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     9781 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     9841 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     9901 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     9961 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
    10021 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
    10081 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
    10141 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
    10201 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
    10261 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
    10321 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
    10381 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
    10441 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
    10501 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
    10561 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
    10621 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
    10681 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
    10741 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
    10801 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
    10861 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
    10921 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
    10981 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
    11041 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
    11101 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
    11161 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
    11221 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
    11281 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
    11341 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
    11401 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
    11461 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
    11521 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
    11581 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
    11641 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
    11701 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
    11761 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
    11821 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
    11881 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
    11941 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
    12001 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
    12061 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
    12121 ttttgtctca ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa
    12181 cgtttttcta tttttaagcg taatttattc attttttatt ctgtatacac tttcaaaaaa
    12241 atatttattc attgaaaaa
//
LOCUS       DAZKCC010000086        12121 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000086 DAZKCC010000000
VERSION     DAZKCC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.088"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..951
                     /locus_tag="ACG8VH_002407"
     CDS             <1..951
                     /locus_tag="ACG8VH_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFP9110942.1"
                     /translation="IKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFD
                     SEVKGLLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSY
                     LVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANA
                     DVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGM
                     TRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEF
                     FKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1023..2144
                     /locus_tag="ACG8VH_002408"
     CDS             1023..2144
                     /locus_tag="ACG8VH_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9110943.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2226..3998)
                     /locus_tag="ACG8VH_002409"
     CDS             complement(2226..3998)
                     /locus_tag="ACG8VH_002409"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110944.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(4001..5713)
                     /locus_tag="ACG8VH_002410"
     CDS             complement(4001..5713)
                     /locus_tag="ACG8VH_002410"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9110945.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5828..6496
                     /locus_tag="ACG8VH_002411"
     CDS             5828..6496
                     /locus_tag="ACG8VH_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP9110946.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6563..7351)
                     /locus_tag="ACG8VH_002412"
     CDS             complement(6563..7351)
                     /locus_tag="ACG8VH_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9110947.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7434..8906)
                     /locus_tag="ACG8VH_002413"
     CDS             complement(7434..8906)
                     /locus_tag="ACG8VH_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9110948.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(9036..10229)
                     /gene="metK"
                     /locus_tag="ACG8VH_002414"
     CDS             complement(9036..10229)
                     /gene="metK"
                     /locus_tag="ACG8VH_002414"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9110949.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10227..10306)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10495..10566)
                     /locus_tag="ACG8VH_002415"
     tRNA            complement(10495..10566)
                     /locus_tag="ACG8VH_002415"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10531..10533),aa:Glu,seq:ttc)
     gene            complement(10578..10651)
                     /locus_tag="ACG8VH_002416"
     tRNA            complement(10578..10651)
                     /locus_tag="ACG8VH_002416"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10615..10617),aa:Ile,seq:gat)
     gene            complement(10664..10734)
                     /locus_tag="ACG8VH_002417"
     tRNA            complement(10664..10734)
                     /locus_tag="ACG8VH_002417"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10700..10702),aa:Gly,seq:tcc)
     gene            complement(10739..10811)
                     /locus_tag="ACG8VH_002418"
     tRNA            complement(10739..10811)
                     /locus_tag="ACG8VH_002418"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10776..10778),aa:Phe,seq:gaa)
     gene            complement(10815..10887)
                     /locus_tag="ACG8VH_002419"
     tRNA            complement(10815..10887)
                     /locus_tag="ACG8VH_002419"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10852..10854),aa:Asp,seq:gtc)
     gene            complement(10906..10979)
                     /locus_tag="ACG8VH_002420"
     tRNA            complement(10906..10979)
                     /locus_tag="ACG8VH_002420"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10943..10945),aa:Met,seq:cat)
     gene            complement(10992..11081)
                     /locus_tag="ACG8VH_002421"
     tRNA            complement(10992..11081)
                     /locus_tag="ACG8VH_002421"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11043..11045),aa:Ser,seq:tga)
     gene            complement(11104..11175)
                     /locus_tag="ACG8VH_002422"
     tRNA            complement(11104..11175)
                     /locus_tag="ACG8VH_002422"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11140..11142),aa:Glu,seq:ttc)
     gene            complement(11197..11284)
                     /locus_tag="ACG8VH_002423"
     tRNA            complement(11197..11284)
                     /locus_tag="ACG8VH_002423"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11248..11250),aa:Ser,seq:gct)
     gene            complement(11309..11382)
                     /locus_tag="ACG8VH_002424"
     tRNA            complement(11309..11382)
                     /locus_tag="ACG8VH_002424"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11346..11348),aa:Ile,seq:gat)
     gene            complement(11396..11466)
                     /locus_tag="ACG8VH_002425"
     tRNA            complement(11396..11466)
                     /locus_tag="ACG8VH_002425"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11432..11434),aa:Gly,seq:tcc)
     gene            complement(11468..11543)
                     /locus_tag="ACG8VH_002426"
     tRNA            complement(11468..11543)
                     /locus_tag="ACG8VH_002426"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11508..11510),aa:Asp,seq:gtc)
     gene            complement(11562..11635)
                     /locus_tag="ACG8VH_002427"
     tRNA            complement(11562..11635)
                     /locus_tag="ACG8VH_002427"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11599..11601),aa:Met,seq:cat)
     gene            complement(11648..11737)
                     /locus_tag="ACG8VH_002428"
     tRNA            complement(11648..11737)
                     /locus_tag="ACG8VH_002428"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11699..11701),aa:Ser,seq:tga)
     gene            complement(11761..11834)
                     /locus_tag="ACG8VH_002429"
     tRNA            complement(11761..11834)
                     /locus_tag="ACG8VH_002429"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11798..11800),aa:Ile,seq:cat)
     gene            complement(11848..11921)
                     /locus_tag="ACG8VH_002430"
     tRNA            complement(11848..11921)
                     /locus_tag="ACG8VH_002430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11885..11887),aa:Met,seq:cat)
     gene            complement(11949..12021)
                     /locus_tag="ACG8VH_002431"
     tRNA            complement(11949..12021)
                     /locus_tag="ACG8VH_002431"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11986..11988),aa:Ala,seq:tgc)
     gene            complement(12041..12114)
                     /locus_tag="ACG8VH_002432"
     tRNA            complement(12041..12114)
                     /locus_tag="ACG8VH_002432"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12078..12080),aa:Pro,seq:tgg)
BASE COUNT         3941 a         2543 c         2101 g         3536 t
ORIGIN      
        1 ataaaaaata agctttcttt gttaaaatta aaagaaaagg aatatattat tgacgatgct
       61 gtttctgtcc ctcttatttt aaataaacga gagaaaaata atttaaagaa aaaaatgctc
      121 attccatttg attcagaagt gaaaggatta ctcaataaac taactatttc taatatagac
      181 tcgactaggg aaaaaatcat aaaaatattt gataaaatta ataatttatt caagaatgac
      241 aatgctgaat taaggaaggg tcttttagag tttaaacttc aagaactaga acttcattac
      301 tcttatttag tgaaaattac cgaagaaaaa gaacaacaaa aagcaataaa agaacagatg
      361 atagaagagg aaaaagttcg tagagaaatt gatcgtgaaa agaaacgtat tgataaagaa
      421 caacgtcaat tcaattctga aatttccaaa ttaatcgcat atatgcaaaa agctaatgca
      481 gatgtggaaa aagaattata tgcaaataaa attcaagaac tggaagaaaa attaaaagaa
      541 ttagaagtta tacgtgaaaa tgtattacaa agagaattaa atactagagc cggttatgtg
      601 tatgtaatta gtaatattgg gtcttttggt gaagacatat ataaaattgg tatgactaga
      661 agattagaac ctatggatag aataaaagaa ttgagtagtg catcggttcc atttgaattt
      721 gacgttcatg cgatgatttt ttcagaggac gctccgtctt tggaaactaa attgcataat
      781 cattttagaa aacaagaagt taataaaatt aatcaaagaa aagaattttt caaagtttca
      841 ctagacgaaa tagagaaagt tgttttagaa aattataatg gaacagtaac atttacaaaa
      901 ttagctaaag ctgaacaata tagacgatct ttagaattgt caaaagatta aatatataaa
      961 aagccttcgg gctttttctt ttaacaatta ataggaacgt atattcgaaa ggaatagaaa
     1021 taatgtggtt agaaaaaata aatgacaaaa aatttaagta caccgaacgt tatacggatc
     1081 ccttaactga aaaaaagaga aaagtgtccg ttactttatc tagcaattca cgccaagctt
     1141 ggaatcaagc taacttactt ttgaatgaaa aaatcgcaga aaaaataaaa cgaaatgaag
     1201 aattaccact aacttttgga gaattaaaaa ctaaatggga tgaaaaatac aagccaactg
     1261 taaaagaaag ttcctatcga acaactcagg tttatctatc attaatttca aaatatataa
     1321 aagatgatgt acttgtaaaa aacgttaata gtaatcttat acaagatatg ctagattact
     1381 actattttga aaaaaattta tcatataatt atgtaataca tttaaaaact tttacaggaa
     1441 tgatttttaa attcgccaac cgtcgatatg gattaaaata taatcccgta aatggggttt
     1501 ctttagcaag aaaacctaag acaaacgaag aaataaaaaa agaaaaagat ggttatttat
     1561 caaaagaaga agttcaaatg attgctagta aacaaaaaag ttcacatcaa cagtcacgtt
     1621 attctttaat tactaaattt ttgtttttaa ctggactacg ttatggtgaa ttgatatctt
     1681 tgactgaatc tgattatgat ggtaataaaa ttacggtttc tggtacttat gattacgagt
     1741 taaaaattaa aacaacaact aaaaatacag gttcttatag aaccattgaa ttatcaagta
     1801 atgctaaaga aatcatagat caattaattg aagaaaacaa attaattaag aataataaag
     1861 ataaatacat tttcatctct aaaaatggta acccgatatc tattcaagca tataatcaat
     1921 ctttaagagc agtatccaaa gaattaaacc tagataaaaa agtttcttct cacatgttaa
     1981 ggcattccca catttctcta cttacagaat taggaatccc attaaaagcc attatggatc
     2041 gtgtaggaca tgaagattca aaaacaacat taaagattta tactcataca actaaaaata
     2101 tgcaaaatca actagtagaa aaactaggaa aaatagaaat ttaaattgcc ccttttctgc
     2161 cccttataaa aacaaacaac ataaaatata taaaaagaac cccgttataa cgaggttctt
     2221 ttaatttaac tttcaaatac aaattgatta tgataaagtt ccgcataaaa tccattttct
     2281 gctaataact cgtgatgact gccttcttca atgacttccc catcacgcaa gacaacgatt
     2341 ctatctgcat tcaagattgt tttcagccgg tgagcaatca caaagctggt tcttcctttg
     2401 attgcttcat ccattgcttt ttggatcctt gcttctgtta ccgtgtctac attacttgtt
     2461 gcttcatcca aaatcaataa agatggattt gtgatgatcg ttcgagcaat actgatcaac
     2521 tgcttttgac ctgtactgaa taagttattt tcttcggata tctctgtatc ataacctttt
     2581 tctaacgtca tgatgaagtc atgaatattc gcttgttttg ctgcattgat gacttcttca
     2641 tctgaagcgt ccggtttgcc gaatgtgata ttgtctttga tcgttccgga gaacaataca
     2701 gattcttgca aaacgattcc tacatgtcta cgtaatgcat caagatcata atctcggata
     2761 tctattccat cgatgactac tgaaccatca ttgacatcgt aaaaacggtt tagcagattc
     2821 atgatcgtcg ttttccccga tcctgttgga ccaacaagag caaccatttc tcctttgtcc
     2881 acattgatcg agacatcttt caagactagt ttttctggag aataaccaaa atctacgtgc
     2941 tgcaacgcaa gacctttttc aacaccatca aatgctttac agttttctgg tttgacttca
     3001 tctttttcat caaatatttc attcaatcgt cttgcccctg taatcgctaa ttggatcatg
     3061 ctgtatccag atgaaatctg catcagtggt tggtaatatt gttgtgaata ctggacgaat
     3121 gtcacaacta agcctaatgc caccgaacgt tccaaatcac catttaatgc taaccagccg
     3181 ccgaaaaaga taactatcgc tgtattgacc agtgacattc cttgcatcat cggaaatagt
     3241 agaccagaat aagcttgtcc tttgaaggtt gcttcgcgaa cactttgatt atgctctaag
     3301 aatccttcaa tcgtttcttc ttgtaagcca tttgtgatga tgacacgttg tccgctgatt
     3361 ttttcatcca tataaccatt tagtttcccc acttcatctt gctgaagatc cacatatttc
     3421 cgtgctttgc ggataatcaa gacagctatc aaaacggcta ctggagtgga agcaattgta
     3481 gcccaagcta actctacatt ttggcggaac atcatgatca agatcccgac aagtaaaaca
     3541 gcattcgtga tgacttgaag caaagcttgg ttcaaactat tttgaatatt atctaagtca
     3601 ctggtaaaac gacttaaaat ttcaccgtct tgatgagaat caaagaaacg gatcgtcaat
     3661 ttttccaatt tgttgaatag accgatacgc attcggttcg ttgacttccc tactacttgt
     3721 gtgaacaaaa tactatagat aaagttggct gcactagcta gtacataaaa cagcaagagt
     3781 ttccatatta cagaaacaaa tttgctttta tcgtctacgc cttgcatcaa gcctcctacg
     3841 taattagcta attcttgaaa agcttccccg atgtattgag gtgcctttac ttgtagatag
     3901 gtagcaatga tcactgtgat aaagataaag aaaaaggaga gtttgtaccg cttcaaatag
     3961 tgatagaaaa atttacttgc ttttattaga tcagtcatac ttactcctcc gttccttttt
     4021 gagtttcaaa gatttcctga tagacacggt ttccagctac caattcctca tgggttcctt
     4081 cgccgactaa tctgccttca tccaatacta agatgcggtc tgctttgaca acagaagcaa
     4141 ttttttgtgc gatgacaatc gtcgttgttc ccttcaaatc tcgatcgagt gcttctcgga
     4201 ccagcttttc agagcgagca tccagtgcac tcgtactgtc atctaggatc aagatctttg
     4261 gattaccgat aacgccacga gtaatggata aacgttgctt ttgaccacca gaaaagttac
     4321 tgctacgttc ttctattggt gcttcgtaac gatcagataa cttttcgatg aattcttttg
     4381 cttgagcaat agaagaagca tgttccatct ctttttctgt tgcattcttt ttcccttggc
     4441 gcaagttttg agcaatcgtt cctgagaata aaattgcttt ttgcaaaaca aaggaaaccg
     4501 tttgtcttaa actcttttca tttacttgac gaagatctac accacctact tcaactttgc
     4561 cttctgtcgg atcaaataga cgagggatca attgtgctag tgttgacttc ccggcaccag
     4621 tcgccccgac gataccaacc atttctccag gtttgataga aaagctgata tctttaagcg
     4681 tatctgtatc ctcaccagga taacgaaaag aaacatggtc aaaagtgaca gaacccgtca
     4741 actcttgttc tggaacgtct aaataagtaa gatctggttc agcatctaaa atttccgcaa
     4801 tccgcttcag cgatacggct gctcttgaag tcatcatcat catcattccg ccaataatga
     4861 ttgccatcat aatctgcatc aagtagttca tgaatgaagc aattccacca attaacgtag
     4921 gatcatcttt aacaagatcg ctcacaaaaa agatcgcccc aaccacagcc aaattggcag
     4981 ccagcatgaa ggacgggatc atcacagaaa ataatgtacc gactatgatg ttgtgttttg
     5041 ttaagtcctc acttactttg ctgaatgact tcaattggtt ttcttcttgt acaaaagatt
     5101 tcactacacg aatccctagt aagttttctt ttgcaatgct attcacacga tcaatcagtt
     5161 tttgaatgat catgaagtgc ttgcccatcc ttgtaaatga aagtgccgta atcagaaata
     5221 ccgtaacgat aagcgctacg atgatccacc ataattgcgg catcgtgatc atagctaaaa
     5281 tgaaggcacc aataaataag aatggaattc gaaaaagtga ttgcaaagaa atcatcacta
     5341 aattttggat ttgcgtgata tcatttgtta gacgaacaac aagatttcca gcagagaact
     5401 gttcgatatt tccaaatgaa aatgtctgga tctttcgaaa tgcttcttct cggatatctg
     5461 cacttacgcc ttgagctact tttgcagaga aaatcgtgtt cgttacacct gcggctaatc
     5521 ctagcaaagc cagactgatc aggtaaatac caattgtttt catttcatca ttatcttctg
     5581 taataatagc ttccaatact tgctgtagta acttaggttg ccataatgct gctgcaacca
     5641 tgactgctac ggacagcaag gaaataaaaa cagctacttt atattttttc gcatgtcgta
     5701 agattaattt cattcgttga ttacacccct ttgtttaaat tgacaggact aatttaacac
     5761 tgtaaaatta gaccgactaa caatttacca tgtttaaaaa gataatgcaa tacacgaggt
     5821 tgataaaatg gaaataaagc tttctcaagc aatcatcatt caggccgcct ttgatatcct
     5881 ggctgaaaca caagctcttt cgcagctttc catgcgaaac atcgcaaaca gaattcatgt
     5941 tcaagcacct gctttatatt ggtatttcaa aaacaaacaa cagctcttgc aaataatggc
     6001 tgaaacaatg gaagaagaat tagcgcttcc agatccaact ttgccttgga atgaacagtt
     6061 gctgcagtat atggaaaatt actatgacct ttatacacgt ttcccttgtg gggcagaatt
     6121 agaaatcaat actgtgcccg cttttccgag tcgattagaa catttggagc aaatgatcca
     6181 attacttgtt cgtgaaggat tttcagtcac ccaaagtcat caggccattg cctctctcca
     6241 caatttattg attggacagt taatggatca gcagcatgaa cagcagcttc gacaaaaaat
     6301 tatggacgga aatgaatttt tgaaagaaac agttctattt atgcgtaatt atgtagttga
     6361 aaatcaatta gagggattta aagaaagtat gcagtcccat gcagaattga atgaaaaagc
     6421 actctttcta acgagtgcac aaacctatat tgaaggctta tgccaacaaa ataaaagaag
     6481 agaaaacgag cagtaaaaaa gctcctatag agatcactat gtttgtgatc tctataggag
     6541 ctatcttttt aagactcttt ttttattttt cttccattag ccacgccaat gcacgttgaa
     6601 tccattggtc ccagaagatc cagttatgat cgccaggtcc ttgttcaaag gtgaccgcca
     6661 agccttgttc ctgcatagca gataccattt cctgatttgc ctgataaaga aaatcagatt
     6721 ccccacaagc catataaaat cttggaactg gttttccgct ttttactagt tctttcaata
     6781 agtattgcgg atcatttttt gaaccttcga tttgatccaa tggtccaaag atcccttccc
     6841 aaaaagatgc tttacgaacc atcaaaagat catccattcg atttcctaca gaaacagcgc
     6901 ctgaaagaga gattactgct gcgaaatttt ctgattttgc aaggcctagt tttaacgcgc
     6961 catatccgcc catcgataat ccagcggcat atgttttttc tctttttctt gttaattgcg
     7021 ggaataattc atgacaaatc tcaggtagct cttctgcaat gaatgtccaa tacttcatgt
     7081 cataaactgt atctgtgtac cagccaagat cagtagatgg catgatgaca gctaatccgt
     7141 agtttgctac ataacgttca atcgacgtac gtctttccca tacactatga ttgccaccca
     7201 ttccatgaag taaatataaa acaggtatat ccgttgtctc ttgctctgaa ctggtgccaa
     7261 tcaccttttt tgttgtttgc ggtagtatca catttagatt gacttccatt tctaacacat
     7321 tcgaataaat atttgcttga ataaatgcca tattttcgcc ttccttagta agaagttatt
     7381 tttgaataac tttaaatttg aatgattcgg ctacgtacat atcttaaccg aaactactgt
     7441 tttattttac cacttttttc atgaacgaaa agcgttaaga ccaaagcaat gatcacgagt
     7501 aacaagccaa ctagataaac ggaatgtaac ccatcataaa gaatttttct taatggtaac
     7561 aaaatatccg catcaatata tttagccgtt tgtggattca ccagtttatt catgatatct
     7621 gcatcatttg ttaaatttgc tgctgcaagt tctcgtttag ttaccccatt tatcaataaa
     7681 ccaaaaatag aaaccatgac tgtttgacca attgttcgaa ctaacgtatt aaaagaagtt
     7741 gctacaccaa gctggtcacg agatacacta ttttgtgcac tgacagtcga ggtagtcatg
     7801 gtcacaccta agcctatacc gatgaccgtt gatatagcaa aaaaccataa atagttactt
     7861 ctgaaaggca taagatacaa ccaagcgccc ccaatagctg cgattagtag tccaattctc
     7921 aagacccaat gagttttcat ttgagtcatc cacttgcttg caaggaaaga accgaccatc
     7981 cagactagtg ataatggtgc taataccagc ccaccaattg ctgcattcag ccctaataca
     8041 ccttgcatcc acatagggat ataaacatcg attcccatca aaaaaccact agccaatgct
     8101 gcagccaagt tgaccacaac aaatagtgga tttttgaata aatgcatcat gatgacagga
     8161 tctttagctc ttttttcggc aaaaacaaaa atacctaaca ttacgatact acctagcagc
     8221 aaagcagaaa caagcggact cattccttcg tctcctaata attggaaccc taacagcaat
     8281 gagacaagca tggccatcaa actaaaacta ccaaaaatat ccattggttt agactcatgt
     8341 atgatttttg gttcatttaa atatagccaa atcaaaataa ttaatagaat tccgatcggc
     8401 acattgataa aaaagatcca gtgccagctg atcgtttcaa cgatgaatcc tcccgcaagc
     8461 ggtccaaaaa cactggcaat cccccaggct gaactattca aacctaaaac ttttgctcgc
     8521 ttttcaatag aataaagatc gccgatgatc gtcaaagcta ctggcatcat tgccccagca
     8581 cccatccctt gaatcgcacg agcagcaatc aacccaatca tgctttgaga taaaccgcaa
     8641 aataacgaac cgataataaa caataatgtc ccaaaaataa agattggttt ccgtcccact
     8701 ttatccgcaa gttttccgta aatcggagta agcatagcgt ttgtcagcaa ataaatcgaa
     8761 aatacccagt tcatgatttc cattccatgt aatgaaccga caatcgttgg catagcggtt
     8821 gttacgattg taccttcaat agccgtcatg aacgttgcaa tgaaaatact cactgtgacc
     8881 aaagtaacat ttgtttcttt tttcatctta ttcctccctt tcattatgac aggatctaaa
     8941 aatagataaa taattactat ctttttcaag aggttaactg ccataaaaaa agctggaaca
     9001 accaccactt ggcgatcgtt ccagctgaat ataacttatt ttgctaaact tgctttcaac
     9061 gcatctactt tgtccgtatg ttcccaaggc aaatctacat ctgtacgacc aaaatgaccg
     9121 taagcagctg tttgtttgta aatcggacga cgcaaatcaa gcatctcgat gattcctgct
     9181 ggccgaagat cgaagttttc tctcactgca gcaatcagtt cttcttccgg aaccgttcca
     9241 gtaccgaaag tattgattga gatcgaaaca ggttgagcaa caccaatcgc ataagctagc
     9301 tgtacttctg ctttgcgagc aagccctgct gcaacaatgt ttttagcaat ataacgtgca
     9361 gcatagctgg cagaacggtc aacttttgtc gcatctttac cagaaaaagc accgccacca
     9421 tgacgagcat aaccgccata cgtatcaaca atgatttttc ttcctgttaa tccggcatcc
     9481 ccttgaggac caccaatgac aaatcggcca gtcggattga tataatattt cgtttgatca
     9541 tctaataatt cagctggaat aacttccttc actacttttt cgatgacatc atgacgaatt
     9601 gtttcattat ctactgcatc atcatgctgt gttgaaataa cgattgtatc tacgcgttcc
     9661 ggttgtcctt gatcatcata ttcaaccgtt acttgagatt ttgcatctgg acgtaaatac
     9721 gttaattcat ttgatttacg caaatctgcc aagcgtcgta ccagacgatg actcaaagcg
     9781 attggtaaag gcatcaattc tggtgtttca tctacagcaa acccaaacat taaaccttgg
     9841 tcaccagcac caatctcatc taatacgtct tttttatctt catcccggat ctcaagtgct
     9901 tcatcaactc cttgagcaat atcaggagat tgttcatcaa tcgcaaccaa tacagctgct
     9961 gtatctccat caaatccgaa ttttgcacgt gtataaccaa tttcttttat tgtttcccgt
    10021 acgacttttt gaatatctac atatgccgtc gtagatattt ctccaaatac taaaacaaga
    10081 ccagttgtta cagacgtttc acacgctact cgtgctgttg gatcttgttt taaaattgca
    10141 tccaagattg catcactgat ttggtcagca attttatctg gatgtccttc agaaacagat
    10201 tctgatgtaa ataagtgtct ttctaccatt ataatgattc cccctagtta aattcggtta
    10261 caaggcatct ccgtcctgcg gatcctttcg ggaacttgca acgaaccaag tatagcagat
    10321 aaaacagttt tctgctcgta ttttttcatt tttttgctta aaaaatataa aaatatttta
    10381 cattagaact gtccaaaaaa gcttagcaca atcatccata aagataactc tgctaagctt
    10441 tttaaaatca ttctttcaga taaaaagaaa aagatactca aaaagtatct tccatgaccc
    10501 gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa
    10561 cgggccaata aaataaatgg gcctaaatgg actcgaacca tcgacctcac gcttatcagg
    10621 cgtgcgctct aaccagctga gctataggcc cataaaaact aaaagcgggt gacgagaatc
    10681 gaactcgcga caacagcttg gaaggctgtg gttttaccac taaactacac ccgcataatg
    10741 gcgcgggaca gaatcgaact gccgacacat ggagcttcaa tccattgctc taccaactga
    10801 gctaccgagc caaacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
    10861 gcatgttaac ccctacacca cggaaccaat gtttttagtt tttaattgcg ggagcaggat
    10921 ttgaacctac gaccttcggg ttatgagccc gacgagctac cagactgctc catcccgcga
    10981 taatatgaaa aaggaggata agggattcga acccttgcac ggttttaccc gcctgacggt
    11041 tttcaagacc gttcccttca gccggacttg ggtaatcctc catgtaaaac tatattaaat
    11101 taatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg
    11161 cttgaccaac gggccaggta aaacaaaata ttgaaacgga gaaggaggga tttgaaccct
    11221 cgcgccggtt tcccgaccta cacccttagc aggggcgcct cttcagccac ttgagtactt
    11281 ccccaaaacc aataagattt tgtactaatg ggcctaaatg gactcgaacc atcgacctca
    11341 cgcttatcag gcgtgcgctc taaccagctg agctataggc ccataaaaaa ctaaaagcgg
    11401 gtgacgagaa tcgaactcgc gacaacagct tggaaggctg tggttttacc actaaactac
    11461 acccgcatgg cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat
    11521 gttaacccct acaccacgga accagtgttt ttagttttta attgcgggag caggatttga
    11581 acctacgacc ttcgggttat gagcccgacg agctaccaga ctgctccatc ccgcgataat
    11641 atgaaaaagg aggataaggg attcgaaccc ttgcacggtt ttacccgcct gacggttttc
    11701 aagaccgttc ccttcagccg gacttgggta atcctccata tgaaacttac aacgccacaa
    11761 tggaccttgt aggactcgaa cctacgaccg gacggttatg agccgtctgc tctaaccaac
    11821 tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg gatcgaaccc ccgacctccc
    11881 gggtatgaac cggacgctct agccagctga gctacaccgc gaaaaagata ttcattattt
    11941 aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc tgcgtgcaaa gcaggcgctc
    12001 tcccagctga gctaaggccc cgtgtttaaa tttataatta tcgggaagac aggattcgaa
    12061 cctgcgaccc cttggtccca aaccaagtgc tctaccaagc tgagctactt cccgtttaat
    12121 t
//
LOCUS       DAZKCC010000087        11603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000087 DAZKCC010000000
VERSION     DAZKCC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.089"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8VH_002433"
     CDS             6..305
                     /locus_tag="ACG8VH_002433"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9110950.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8VH_002434"
     CDS             378..1739
                     /locus_tag="ACG8VH_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9110951.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8VH_002435"
     CDS             1761..2021
                     /locus_tag="ACG8VH_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110952.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8VH_002436"
     CDS             2035..2787
                     /locus_tag="ACG8VH_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP9110953.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8VH_002437"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8VH_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9110954.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8VH_002438"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8VH_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP9110955.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8VH_002439"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8VH_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9110956.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8VH_002440"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8VH_002440"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9110957.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8VH_002441"
     CDS             7161..8558
                     /locus_tag="ACG8VH_002441"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9110958.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8VH_002442"
     CDS             8639..9079
                     /locus_tag="ACG8VH_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9110959.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8VH_002443"
     CDS             9227..9553
                     /locus_tag="ACG8VH_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9110960.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8VH_002444"
     CDS             9554..10303
                     /locus_tag="ACG8VH_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP9110961.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8VH_002445"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8VH_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9110962.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8VH_002446"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8VH_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110963.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3662 a         2014 c         2357 g         3570 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa aca
//
LOCUS       DAZKCC010000088        11448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000088 DAZKCC010000000
VERSION     DAZKCC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.090"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..54)
                     /locus_tag="ACG8VH_002447"
     CDS             complement(<1..54)
                     /locus_tag="ACG8VH_002447"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9110964.1"
                     /translation="MLVFQMRNVDKTSTVLKQ"
     gene            complement(103..342)
                     /locus_tag="ACG8VH_002448"
     CDS             complement(103..342)
                     /locus_tag="ACG8VH_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9110965.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(476..1693)
                     /locus_tag="ACG8VH_002449"
                     /pseudo
     CDS             complement(476..1693)
                     /locus_tag="ACG8VH_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2184..3122
                     /locus_tag="ACG8VH_002450"
     CDS             2184..3122
                     /locus_tag="ACG8VH_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9110966.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            3252..3899
                     /locus_tag="ACG8VH_002451"
     CDS             3252..3899
                     /locus_tag="ACG8VH_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFP9110967.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(4592..5530)
                     /locus_tag="ACG8VH_002452"
                     /pseudo
     CDS             complement(4592..5530)
                     /locus_tag="ACG8VH_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5530..6147)
                     /locus_tag="ACG8VH_002453"
     CDS             complement(5530..6147)
                     /locus_tag="ACG8VH_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9110968.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(6161..6331)
                     /locus_tag="ACG8VH_002454"
     CDS             complement(6161..6331)
                     /locus_tag="ACG8VH_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110969.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(6679..8169)
                     /locus_tag="ACG8VH_002455"
     CDS             complement(6679..8169)
                     /locus_tag="ACG8VH_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9110970.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(8212..8511)
                     /locus_tag="ACG8VH_002456"
     CDS             complement(8212..8511)
                     /locus_tag="ACG8VH_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110971.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8877..9161)
                     /locus_tag="ACG8VH_002457"
     CDS             complement(8877..9161)
                     /locus_tag="ACG8VH_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9110972.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(9680..10165)
                     /gene="vanZ-A"
                     /locus_tag="ACG8VH_002458"
     CDS             complement(9680..10165)
                     /gene="vanZ-A"
                     /locus_tag="ACG8VH_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9110973.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10318..11229)
                     /locus_tag="ACG8VH_002459"
     CDS             complement(10318..11229)
                     /locus_tag="ACG8VH_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP9110974.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3508 a         2005 c         1744 g         4191 t
ORIGIN      
        1 ctgtttcaaa acagtagatg ttttatctac attacgcatt tggaatacca acatgacgaa
       61 tccctccttc ttaattacaa atttttagca tctaatttaa cttcaattcc tattatacaa
      121 aattttaaga taatgcacta tcaacacact cttaagtttg cttctaagtc ttatttccat
      181 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc
      241 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc
      301 atttcgtttt tctttttgtg cgcctaaatt tcccacaatc actcacttct ttctatttct
      361 tcttattctt attttatcat caacaatcac aaatcacttg tgatttgtga taagtgattt
      421 gtgataagtg atttgtgatt aatatataaa agccctcttt aaagggcttt tatgtttatt
      481 ttgagaaaga tataaaatca atatatccct tttccccaat ttttacaacg gcattgtagg
      541 gctttttctc tttgtttttg attcctttta ccagggtttc ttttccctcc agtaattctt
      601 ttacattcgt tttggttagt tttttctttc taaaatgttc agctaaagta aacttacatt
      661 caggataatt tgaacaacca taaaacgatt tttttaatac aatattgttg ccacacttag
      721 gacattttcc tacaatactt ttttctgctt ctttttcttt ctgttcctgg taatcagaaa
      781 aatttagttt ttctatatcg ttaggtacag cttccagtaa atgaacaatg aattttttga
      841 tattcgtaat aaagttctct tgattgcctt ctcttttacc gatttttttt aaatacgttt
      901 cccatttagc cgtcatttca gcactcgtta aaaggtgctg actttcaact gcctggcaca
      961 ataattttcc tttttcagtt acaacaagct tattcttaat cacttggata tattcttttt
     1021 gttttaacgc ttcaataata cttgctcttg tcgcttctgt tccaatcccc tcaacttctt
     1081 gtaaaatctt gattgcttct tcatcatcaa ccgttttatt cgccgttttc atagcagtta
     1141 ataatgtacc ctctgtaaaa gcttttggcg gttgcgtttc tttttcaaca ctcttaacac
     1201 caacttcggc acgttcgcca atgataacga gtggtaacgt ttgaacgtcc tctttttctt
     1261 ctttggtttg ttgtttgaaa agaattttcc acccttcttg ctttggtgtc tttccgatcg
     1321 attgaaacag caaatcagct actttcgttt gtatcttggt ttcttcatac aagtaatcag
     1381 gtaaaaacat ggcaacggtc gttttaacga ctaaagcata aatttttcgt tgtaaatcgt
     1441 ccattttcgc taatgcagat tcggtaggta cttgttttgt tgggataatg gcgtggtgtt
     1501 cctgtacctt actaccgtcc acataacgct ttctaggctc tgtttgaacc atttcaagat
     1561 caagtcctaa aaaaccgcta tatttgccaa aattcgcttt taaataagca aattcgttct
     1621 ctgtaataaa tggtgtatct gttcttggat aactcaataa ttttgcttca tacagtcctt
     1681 gcatagcttt taaacgtaaa attttgcgtt ctaagacgtt ttaaatatat ctgcgtatat
     1741 ttttatgtgt tttatctttt cgtcggcaaa tgcgttaaaa tttgcgaaat aaaatagctc
     1801 aagggcgaac gcagtgagtg catttaccct tgatctattt ttttgagtaa attagctttt
     1861 gaaaccgacg gaaaggaaac acaaaacttt tcagaggcaa ggggttttta aggggcgctt
     1921 ttagcccctt gaatttattt gctttaagat atatttagtt ttaaaatagt tttaacgaaa
     1981 aaaagacata aaaaaagacg agcaacgcta ccaacgttct cgcccggaat aacaaaccac
     2041 acttgttatt cattcgatga ttagtatatc aatttttgat tttgaataca aaacaaaatg
     2101 gacgaatgga tacaggcgaa atttattttg cgattccact cgtccttttt tcgttccaaa
     2161 aattatttta ggagcgataa aaaatgaaga aaattgatga aaaattagct gcagaaatct
     2221 cactaaaaaa accggccgtc tctaatagcc ggttaagtaa tagccggtta agtggtcaaa
     2281 ctttgggaaa atctcaaccc gcattaagtt ttgatgccat gacaatcgtt ggaaatttga
     2341 acaaaactaa tgctaaaaag ctatctgact ttatgagtgt agagccacaa atacgacttt
     2401 gggatatact tcaaacaaag tttaaagcta aggcacttca agaaaaagtt tatatcgaat
     2461 atgacaaagt aaaagcagat acttgggata gacgtaatat gcgtgttgaa tttaatccca
     2521 ataaactcac acatgaagaa atgatttggt taaaacaaaa tattatcgac tacatggaag
     2581 atgacggttt tacaagatta gacttagctt ttgattttga agatgatttg agcgattact
     2641 atgcaatgac tgataaagca gttaagaaaa ctgtttttta tggtcgtaat ggcaagccag
     2701 aaacaaaata ttttggtgtc cgtgatagtg atagatttat tagaatttat aataaaaaac
     2761 aagaacgtaa agataacgca gatgttgaag ttgtgtctga acatttatgg cgtgtagaag
     2821 ttgaattaaa aagagatatg gttgattact ggaatgattg ttttaatgat ttacacatct
     2881 tgaaacctgc gtgggctact ttagaaaaaa ttaatgagca agctatggtt tatactttgt
     2941 tgcatgaaga aagtatgtgg ggaaagctaa gtaagaatac taagactaaa tttaaaaaat
     3001 tgattagaga aatatctcca attgatttaa cggaattaat gaaatcgact ttaaaagcga
     3061 acgaaaaaca attgcaaaag cagattgatt tttggcaacg tgaatttagg ttttggaagt
     3121 aaaataagtt ttatttgata aaaattgcta attcagtata attaatattt acgaggtgac
     3181 ataacgtatg aaaaaatcag aggattattc ctcctaaata taaaaattta aaatttagga
     3241 ggaagttata tatgactttt aatattattg aattagaaaa ttgggataga aaagaatatt
     3301 ttgaacacta ttttaatcag caaactactt atagcattac taaagaaatt gatattactt
     3361 tgtttaaaga tatgataaaa aagaaaggat atgaaattta tccctcttta atttatgcaa
     3421 ttatggaagt tgtaaataaa aataaagtgt ttagaacagg aattaatagt gagaataaat
     3481 taggttattg ggataagtta aatcctttgt atacagtttt taataagcaa actgaaaaat
     3541 ttactaacat ttggactgaa tctgataaaa acttcatttc tttttataat aattataaaa
     3601 atgacttgct tgaatataaa gataaagaag aaatgtttcc taaaaaaccg atacctgaaa
     3661 acaccatacc gatttcaatg attccttgga ttgattttag ttcatttaat ttaaatattg
     3721 gtaacaatag cagcttttta ttgcctatta ttacgatagg taaattttat agtgagaata
     3781 ataaaattta tataccagtt gctctgcaac ttcatcattc tgtatgtgat ggttaccatg
     3841 cttcactatt tatgaatgaa tttcaagata taattcatag ggtagatgat tggatttagt
     3901 ttttagattt tgaaagtgaa tttaatttta tacacgtaag tgatcataaa atttatgaac
     3961 gtataacaac cacatttttt ggttgcttgt ggttttgatt ttgaatttgg ttttgaactt
     4021 atggactgat ttattcagtc cattttttgt gcttgcacaa aaactagcct cgcagagcac
     4081 acgcattaat gacttatgaa acgtagtaaa taagtctagt gtgttatact ttacttggaa
     4141 gatgcaccga ataaaaaata ttgaagaaca actagcaaaa gattttaaag agttatttta
     4201 ttttaagtct ttataacatg agtgaagcga atttttaaat ttcgatagaa atttttacat
     4261 caaaaagccc cctgtcaaaa ttgacgaagg gggttttttg gcgcacgctt ttcgttagaa
     4321 atatacaaga ttgaaaatcg tgtataagtg cgccctttgt tttgaactta gcacgttaca
     4381 tcaatttttt aaaatgatgt ataagtgcgc ccttttaaat tttgagtgat tatatttttt
     4441 gagttagaaa aagggattgg gaaaatttcc caaaataatt taaaaaataa gcaaaaattt
     4501 tcgatagaga atgtgctatt ttttgtcaaa ggtgtatacc ttgactgtgc ttgctgttac
     4561 attaagttta tttttaagtt attaaaaaag aaatagcttt taaagtttgg ctcgctgtcg
     4621 ctttataaag ctgattgact tttgattgca aactacttaa agaaaacaaa ctcggactat
     4681 tcgttttctt ctctttggtt tgaacatcag caattatccc ctcttgattg cctattttag
     4741 cttgtttaga agaaacaaaa gctaaaagct cctcttgggt tttaaaacgc tgtgtggggc
     4801 ttagaacgcc cttaaacgac ccttggttta cttttatact agcttccacc tcgaaaaaag
     4861 gttctttttt aaaattctct atggcttcct ggcgctgaaa aataaggtat aaggtgggcg
     4921 tttgaacacg tcctagtgaa aatgtacctt gtacgccctt ctgttgtaaa tttaacgtat
     4981 acaaagggct tgcgttcatg ccgatcaacc aatcggcaat ttggcgtgtt tgcgcttctt
     5041 gataaaaggg atagtaattc attccaggtt gcaaattttg aaaaccgctt cggattacat
     5101 ctttttctaa gctattgatc catagtcttt taaatgtttt atcttttgaa aaggcatttg
     5161 ctttatggat aatcgaccag gcgatatttt caccttctct gtcgctatct gttgcaacaa
     5221 taattgtatt tgcctttttg agaagttctg caacaatttt aaactgcttt cccttatctt
     5281 ttgcaacttc aaaatcgtat cgatcaggaa aaatcggcaa agattcaagt ttccaatttt
     5341 gccacttttc gtcataatga cctggttctg ctaattccac taaatgccca aaaccaaagg
     5401 tgataaacgt ttcatctgta aatagtgggt ctttgatctc aaaataaccg tcttttttgg
     5461 tgctttgttt taaagcactt gcgtaggcta atgcctggct tggtttttca gctaaaataa
     5521 ccgtactcat taactatccc tcttttcatt gttttttctt tgatcgactg tcacgttata
     5581 tcttgctcga taccttctaa acgttcggcg attgattcca gtttgttctt caacttcttt
     5641 atcggataaa ccattcaaaa acaaatcgaa agcatgcttt aaacgtggat cattttcttt
     5701 aaatttatgc tgacgacctt tgaatttgcc ttttttctta gcaatttcga ttccttgtgc
     5761 ctgacgttcc ttaatttttt ttcgttctga ttctgcttga tacttgtaca attcaatgac
     5821 aaggctatta atcaaacgcc ttaaattttc atcttcaata ccattcattg agggtaaatt
     5881 taagacttcc agggttgccc ccttaatttg aatttgattc atcaattctg ttaattcttt
     5941 attatttcgt cctaatcgat ctaattcagt aacaataaca atatcccctt cacgaatata
     6001 gttaagcata gcttgtaatt gtgggcgttc gaccgattga ccgcttaatt tgtctgaaaa
     6061 gaccttagaa acgccctgta acgcttgtaa ttgccgatct aagttctgtt ctttgctact
     6121 gacacgtgca taaccaattt tagccatttt caaccaacct ctaaaattct ctcggttgca
     6181 ataaccaatc agcaatatct actttttcaa tttcaaattg cttatcagaa attgtctttt
     6241 cgtaagcgat aaaatcttgc gcatattgtt gctcattaaa aatagccacc acttcgtcat
     6301 tttctaaaac tcgataaata aattttttca ttttactcct cctattatgc ccaacttaaa
     6361 tgacctattc accaagtcaa ttatactgct aaaatcatat taggacaaat aggtatactc
     6421 taatgaccta taaaagatag aaaattaaaa aatcaagtgt tcgcttcgct ctcactgccc
     6481 ctcgacgttt tagtagcctt tccctcactt cgttcagtcc aagccaacta aaagttttcg
     6541 ggctactctc tccttctccc cctaataatt aattaaaatc ttactctgta tatttctgct
     6601 aatcattcgc taaacagcaa agaaaaaaca aacacgtatc atagatataa atgtaatggc
     6661 atagtgcggg ttttattttc agcctgtatc atagctaaac aaatcgagtt gtgtgtccgt
     6721 tttagggcgt tctgctagct tgtttaaagt ctcttgaatg aatgtatgct ctaagtcaaa
     6781 agaatttgtc agcgccttta tatagctttc tttttcttct ttttttactt taatgatcga
     6841 tagcaacaat gatttaacac tagcaagttg aatgccacca tttcttcctg gtttaatctt
     6901 aaagaaaatt tcctgattcg ccttcagtac cttcagcaat ttatctaatg tccgttcagg
     6961 aatgcctagc acttctctaa tctctttttt ggtcgtcact aaataaggct tgtatacatc
     7021 gcttttttcg ctaatataag ccattaaatc ttctttccat tctgacaaat gaacacgttg
     7081 acgttcgctt ctttttttct tgaatttaaa ccacccttga cggacaaata aatctttact
     7141 ggttaaatca cttgataccc aagctttgca aagaatggta atgtattccc tattagcccc
     7201 ttgatagttt tctgaatagg cacttctaac aattttgatt acttcttttt cttctaaggg
     7261 ttgatctaat cgattattaa actcaaacat attatattcg cacgtttcga ttgaatagcc
     7321 tgaactaaag taggctaaag agagggtaaa catgacgtta ttacgcccta ttaaaccctt
     7381 ttctcctgaa aatttcgttt cgtgcaataa gagattaaac cagggttcat ctacttgttt
     7441 tttgccttct gtaccgctta aaaccgttag acttgaacga gtaaagccct tattatctgt
     7501 ttgtttgaaa gaccaatctt gccattcttt gaaagaataa cggtaattag gatcaaaaaa
     7561 ttctacattg tccgttcttg gtatgcgagc aataccaaaa tgattacacg ttagatcaac
     7621 tggcaaagac tttccaaaat attctcggat attttgcgaa attattttgg ctgctttgac
     7681 agatttaaat tctgattttg aagtcacata gactggcgtt tctaaaacaa aatatgcttg
     7741 ataaccttta tcagatttga taatcatagt aggcataaaa cctaaatcaa tagcggttgt
     7801 taaaatatcg cttgctgaaa tagtttcttt tgccgtgtga atatcaaaat caataaagaa
     7861 ggtattgatt tgtcttaaat tgttttcaga atgtcctttc gtgtatgaac ggttttcgtc
     7921 tgcatacgtt ccataacgat aaacgttggg tgtccaatgt gtaaatgtat cttgattttc
     7981 ttgaatcgct tcctcggaag tcagaacaac accacgaccg ccaatcatgc ttgattttga
     8041 gcgatacgca aaaatagccc ctttgctttt acctggcttg gtagtgattg agcgaatttt
     8101 actattttta aatttgtact ttaacaagcc gtcatgaagc acagtttcta caacaaaagg
     8161 gatattcatt cagctgttct cctttcctat aaatcctata aaataggttg tttaattaac
     8221 ttggtttgct ttttcattca actgtttcaa tattgcatgt tttgaaaaag atttttttcc
     8281 tttataagtc aatttttttc cactaatcga ataaattatt ttgttatttt ctattaactt
     8341 atatatataa tcttccccct ccgaagaaaa atacttatct gattttgttt ctaagtagat
     8401 atttctcttt tctaactctt tcttaaacgt ttctagtgta tagatatttg ctaattttct
     8461 tatctccaat aaactatttt ttatataagt tttacattca tcatgattca tacaaactcc
     8521 accttctata aatgaataca aaaaaagcaa tcaaacgatt tccgattgat tgcttaacaa
     8581 ttcttaaatt cagtagctta gatacttgaa aactctctga tttccctata taatgatagt
     8641 acggttatat accgtcttca aacaaagtta attaaataac ttcttacgag ggaagagttc
     8701 atctgactaa ctgataagcg ttggtttggc aatcttatcg ggctatgcat ttataaaatg
     8761 tcgtcaaaca ttttataaat gtgtcatggc tcttttttcg tttctattca gttcgttgtt
     8821 tcgttatatc tagtataccg cttttaaaaa aaataagcaa cgatttcgtg cattattcac
     8881 acgaagtcat tgcttttttc ttcttccatt tctaaatcca atgttacttg ttctgattct
     8941 gtttctggtt ctggttctgt tggctcattt gggattaaat ccactactag cgttgagtta
     9001 gttaactttg caatttgttc tagtgttttt atggttggat ctgattttcc tgattctatt
     9061 cgtgaataat ttgatctact catttctaat tcttgggaga atttttcttt tgatttggta
     9121 cctctcatct tttttaagct ttctctaaat gctagttcca agtttctccc ccctcttttt
     9181 gtgtaataaa attcacgctt tatttaaatt atagctttta atatcttaat aaacaagtga
     9241 ttaatcactt gtgatttgtg attattccaa aagtaggtaa tttcaccaac ctttttcaaa
     9301 agtttttatc gacgattatt ggactggatc gtgtttccta aagcgattct aagcgcatgt
     9361 ttaattttaa agtgggtaat tctctttaaa acgtcctaca aagattcaaa aaaagcatgg
     9421 ctgctagtgg gggtaccgtc aggaaaatgc ggatttacaa cgttaaggaa atttattaaa
     9481 gagaccaatt cctcagcact aaaattgata attgggcttt ctttgttact atagtgttag
     9541 gtaagctttt tactttatca ccggttttcc cctcacttca cacctacggg cgagtttccc
     9601 cacatcccgt tttccatcta atccaattca ttcaatatct ttatgtgtat gaaagatcag
     9661 attgcttgat ttagacatct tacacgtaat ttattcttaa atgggtacgg taaacgagca
     9721 ataatacgag caaaagtata cctacaaaaa taataactct gtctaatttt ttttgattca
     9781 tatgcttatt gcttaaacca tataatttca gtccaagaaa gcctccaaca gtatttgtaa
     9841 ttacatctgt tatgtctgtc gctccaatag cgaagataaa ttgaattatt tcaaaagtaa
     9901 gacttaaaac cagtacaaaa gcaaacttag gtaaaaatcc gatttcttta aaattgacat
     9961 tcaaaagcaa gccaaatgga ataaagatta taacattatc tatcatctct ctgaaattcc
    10021 cagtagcagt aaatggagtc aagttaagac ttctttgatg ataattaaat actgataaaa
    10081 tattgtattg taatttgaat aacactaacc agattagtgt cactaaatat aaagctagca
    10141 atcctctaga taatattttt cccaatataa tttcctctaa ttcttttgtt taagttgtat
    10201 cattagtata gcaatgtcct agttaacgtg ggttttaaat taaaaagcta acgttacccc
    10261 aaaagtgcta gtacaataat gctgttcatt aatttcgttg gtttcattcc gccatcctta
    10321 cctccttgaa ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt
    10381 gtttcctgat atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac
    10441 aggataataa aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc
    10501 ttttaggtaa tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg
    10561 tggtaaacca acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt
    10621 gtcctctgga taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc
    10681 ttcaggggct cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt
    10741 atgctcacta taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact
    10801 ttgctcatca aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac
    10861 agcatcattg accatctctg aaaatttttg tgctatttct tttgacatat aaatattact
    10921 atcaagcaac ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga
    10981 cttcacactt tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata
    11041 aacctgttct tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa
    11101 atgttctttg ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc
    11161 ttcattaacg tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa
    11221 cttcttcatt ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa
    11281 tcttcccctt aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca
    11341 gtcaattatt tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag
    11401 tgatacccat gctgaattta acgaggctga actgcctgtt gacaatgg
//
LOCUS       DAZKCC010000089        11339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000089 DAZKCC010000000
VERSION     DAZKCC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.091"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1484)
                     /locus_tag="ACG8VH_002460"
     CDS             complement(366..1484)
                     /locus_tag="ACG8VH_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110975.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1717..1839
                     /locus_tag="ACG8VH_002461"
     CDS             1717..1839
                     /locus_tag="ACG8VH_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110976.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1955..3262
                     /locus_tag="ACG8VH_002462"
     CDS             1955..3262
                     /locus_tag="ACG8VH_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP9110977.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="ACG8VH_002463"
     CDS             3381..4580
                     /locus_tag="ACG8VH_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9110978.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="ACG8VH_002464"
     CDS             complement(4607..5875)
                     /locus_tag="ACG8VH_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP9110979.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5930..5996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6038..6684)
                     /locus_tag="ACG8VH_002465"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="ACG8VH_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="ACG8VH_002466"
     CDS             complement(7092..7595)
                     /locus_tag="ACG8VH_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9110980.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="ACG8VH_002467"
     CDS             complement(7718..8482)
                     /locus_tag="ACG8VH_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP9110981.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="ACG8VH_002468"
     CDS             8590..8865
                     /locus_tag="ACG8VH_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9110982.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="ACG8VH_002469"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="ACG8VH_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9110983.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10611)
                     /locus_tag="ACG8VH_002470"
                     /pseudo
     CDS             complement(10432..10611)
                     /locus_tag="ACG8VH_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3401 a         2387 c         2002 g         3549 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaata
//
LOCUS       DAZKCC010000090        11225 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000090 DAZKCC010000000
VERSION     DAZKCC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.092"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8VH_002471"
     CDS             134..307
                     /locus_tag="ACG8VH_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110984.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="ACG8VH_002472"
     CDS             947..1420
                     /locus_tag="ACG8VH_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP9110985.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="ACG8VH_002473"
     CDS             1429..1656
                     /locus_tag="ACG8VH_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9110986.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2292..2663
                     /locus_tag="ACG8VH_002474"
     CDS             2292..2663
                     /locus_tag="ACG8VH_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9110987.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2919..3161)
                     /locus_tag="ACG8VH_002475"
     CDS             complement(2919..3161)
                     /locus_tag="ACG8VH_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9110988.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3193..4095)
                     /locus_tag="ACG8VH_002476"
     CDS             complement(3193..4095)
                     /locus_tag="ACG8VH_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9110989.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4108..4296)
                     /locus_tag="ACG8VH_002477"
     CDS             complement(4108..4296)
                     /locus_tag="ACG8VH_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9110990.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4310..4873)
                     /gene="amaP"
                     /locus_tag="ACG8VH_002478"
     CDS             complement(4310..4873)
                     /gene="amaP"
                     /locus_tag="ACG8VH_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9110991.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4911..5804)
                     /locus_tag="ACG8VH_002479"
     CDS             complement(4911..5804)
                     /locus_tag="ACG8VH_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9110992.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5882..6805)
                     /locus_tag="ACG8VH_002480"
     CDS             complement(5882..6805)
                     /locus_tag="ACG8VH_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9110993.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6854..7204)
                     /locus_tag="ACG8VH_002481"
     CDS             complement(6854..7204)
                     /locus_tag="ACG8VH_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9110994.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7237..8139)
                     /locus_tag="ACG8VH_002482"
     CDS             complement(7237..8139)
                     /locus_tag="ACG8VH_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9110995.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8132..8989)
                     /locus_tag="ACG8VH_002483"
     CDS             complement(8132..8989)
                     /locus_tag="ACG8VH_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9110996.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9323..10132
                     /locus_tag="ACG8VH_002484"
     CDS             9323..10132
                     /locus_tag="ACG8VH_002484"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9110997.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10172..10669
                     /locus_tag="ACG8VH_002485"
     CDS             10172..10669
                     /locus_tag="ACG8VH_002485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP9110998.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11054..11127)
                     /locus_tag="ACG8VH_002486"
     tRNA            complement(11054..11127)
                     /locus_tag="ACG8VH_002486"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11091..11093),aa:Ile,seq:cat)
BASE COUNT         3294 a         2098 c         1879 g         3954 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
     1801 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
     1861 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
     1921 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1981 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     2041 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     2101 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     2161 cattattttg ataataatga ttatttattt aaaaatacat tagataacat ttgttacgac
     2221 acgaaaaagc ttcacaaaca aatatcgtta ttttaaaatg agggcgaact cacacaatta
     2281 ggaggaatag tatgccattt attgtttact tttttctttc attactcgtg atctatattc
     2341 cattaccaac atttttcctg ctcgtcaatc gtctagcaga gcaacatgat acgattgctg
     2401 ctcttacgaa actttgttta cttctatttg tttgtctaga ttactttacg agctattatt
     2461 ggctaaaaca gacaagagta aaaaagagat attatttctt ccttgtcttt ttcaatctct
     2521 ttgagtttct gatccattgc tttttacttc atcagtatca tacgatccat ttgtggatca
     2581 tctgggtttt caaagtggtc gtattatttt gtatgttcac ctttcctatc tcaagaaaat
     2641 ttagggaggc attgttttat taaagaagct tctctgtatc aataaagaca catttaagga
     2701 aaaactttta aaaatctaat tgggaatttg cccgcataaa taagaggctg tgacaaaagt
     2761 cttgtcacag cctcttattc cgtataaaca gtgttcaaaa gcttgcatct tcgataatat
     2821 gcccaaccaa atgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg
     2881 aagaacagct ttttcacgac ctctttttaa aagtcttttt acagttttcg agcaaagaat
     2941 gatacgatta gtactaaaat gatggcacca atcaatgatg gcacgattgc cattcctgca
     3001 atacttggtc cccaagaacc taataatgct tgaccaagtg ctgatccaac taaacctgct
     3061 acgacatttg caataatccc catcgaagaa cctttattcg tgattgctcc tgcaattgct
     3121 ccgataattc ctccgacgat aagtgaccaa ataaatccca tgtttcgttc ctcctaatgt
     3181 tttacttcct tattactgaa cccgtggttc tgtattttct tttacttttt gtgcgccgct
     3241 tccagctgct gattttactt tatctgtttg attcgaagca aattcaccgg tttttttcgc
     3301 tgcgtcagtt actttgtctt gcaccgtttc ttgatctttt tcaaattctt cttttgtttt
     3361 gatgtccact acgttagcgt tgacttcgat cacttctaaa tgtgtcatat tttttacttc
     3421 tttgccgatt acttcttgca tttcttcaaa gattttttcg atatctttgc catattccac
     3481 aatgacatcc atatcaactg ctacttgttt tttaccgact tctgtttcga tcccagccgt
     3541 tacattatct gtattcacaa gttttcctgc cacattagag aagaaaccac cgtttgcaga
     3601 taataaaccg tctacttgtt caattgcgat tccgatgatt ttttgcacaa ctttatcttc
     3661 ataattcagc tcaccgcgca ttgaatcttc ctgttcagaa tcatgctttc cttggttatt
     3721 tgtatgatca gaaccaagtt ttccttgtcc gtttgtgtgg tcagaactaa gtttttcttg
     3781 tgctttcgca tggtcagaag gatgtttttg ctcttccaca ggtttagaag gatcgattgc
     3841 atcatgttgt tctggaactt tagattctgg agttcctgga tgatgaggtg catgcttttc
     3901 tgcatcatgt ttcttctctt ctactccttg ttgaacgtta gggactactg gatctggatg
     3961 tgcagatact ggtgctgttt tgttcttttc atcgttcaca ccatttgatt ggtttggtgt
     4021 cgttgggtct ggatgtgcag acactggttt ttggttgttc attttattgt tattattttt
     4081 gttattgtta ttcatggatg tttcctccta agatttaaaa tagtgatcta agattttcgt
     4141 ttgtttcaga taaaaaccga tagcgacgcc gatcgctgcc atgatcaaga tcaataaggt
     4201 cttgaaaaag cccacggtga tccataaaac ggctaatatc aatccaagta ccccaaagat
     4261 gatagggagc ttgtatgtcg aaaatagttc tttcattttt ggtccccccc tattctactc
     4321 gtttgcggtc attgcttcgt ttagctggtt cttcaaagcc tgtgtatttc accgatactt
     4381 tcactggctc ttttgttccc aatatgtgtt gtacttcatt ttcgatttct ttcatcaatg
     4441 ttcctgtttt accaatgagt gctgaagttc gtttcaactc cccttttaca ttgacggcga
     4501 tcttgttttt cgttgctttg acatttactt ttggtgaatg gatgaattca tcttcatgca
     4561 agtgagaacg aaccattcct tcaattgctt ttttatccaa cgtcaactcc ccatcaccat
     4621 gtttcagtag aaagtttccg attgtttttg gataaaacag gaaaacaagt aacgtgataa
     4681 ttgtcaaaat agcaaatgca acggcaagcc aaaatacata ttgttgcacg tatgaattag
     4741 tgatggaaaa gaatcgaaac ttctctagct gaaaaggcaa tggataggtt gcacttaata
     4801 gtatcgcaaa caaaagcaca gatagcaaaa gtaatgttcc gatcactccg atcgttttta
     4861 cacctttatt cattttcgca cctcctcata ggggtgaaac gatcaacttc ttataagttg
     4921 atcgcttctc caccagttac tccgtaaatt tgggctgtaa cataacttgc tttattcgat
     4981 gctaagaaaa cataaactgg tgctaattct cctggctgac ctgcacgttc tagtaaactt
     5041 tgctgaccaa attcagggat cgaatcagaa ggttgtcctt cgtctagttg taatggtgtc
     5101 cagattggac caggtgctac accatttaca cggattcctt taggtgcaag ctgttttgct
     5161 aaaccgatcg taaagttagc gattgaagct tttgttgctg cgtaatctaa taaatgttcg
     5221 cttggattga atgcttgaac agaagttgtt gttacgattg ctcctcctgc tgggatatgc
     5281 ggaacagctt ctttcactaa agcaaacata gcgatgatgt ttatcataaa tgtgtctttg
     5341 acttgctcaa gtggtaactc agcaatagac tgacaagaaa tttgctgtcc tgcgtttagt
     5401 accatcgtat ctagcccgcc aagttcactc acggctttat ctatgatttc tttaggtgct
     5461 tgttcgtcac gaagatcaaa aggtagtaag acaatcttac gtcctgcatc tttcacgtat
     5521 tttgctactt cctctgcatc tttttcttct cctgggaaga attgaagagc gatatctgcc
     5581 ccttctctag caaatgcaat cgctgctgca cgaccgatcc ctgaatctcc accagtgatt
     5641 agtacacggc ggttttctag ttgattattg ccttcatagg attcttctcc acaatcagga
     5701 acaggaatca tattctgctg taaagctggt gtatcctgat cttgtttagg aaatttctcc
     5761 gtatggtaca agtttcgcgg gtcgattaat tttggttctg tcattgctgt attcctccta
     5821 gtttttaaat tgtattgcca aaacaaattt ctttgtttga tacctgtttt aaatagattc
     5881 tttaatttgt ataatctttc cggtataaat ggtaacctaa aactactgct gctatcaaag
     5941 agccaaagac tacgagtata aagcctagag cagaactttt acccggtaat cctcctgtat
     6001 tcattcccca aatccctgcg aacaacgccg gaacggaaat gattaatgca gctgaatcta
     6061 gatatttcat gatatgattt agattattgt ccatcatccc gttgaataga tcaccaatac
     6121 tctctaataa atctcgatag atctcgatca ttttttctgt ttgtcgctgt ctcaaatgga
     6181 catcatagag tagctgttca tcgtctaaac gtttgatgaa agtttcagaa ttccataatt
     6241 gatccattgt ttcccgttga tctcttaagg tatgatcgat atatacgatg tctctttcaa
     6301 gatctgcctg tttatacagt tcttcatttt ccgttgtttt acgagctgct ttatccaaag
     6361 cgtcaattcg ctttttcatc tctcttagtt ctttcacgta atgggtgtaa atatctaata
     6421 atgtatacgt gatgatcttt tcaaacgagt ttaaatgatt tccatgcttt gtcagcagcc
     6481 gatccacaaa ttcggttttt ctatcgatac aagtaatcaa gagattatcg gaaagtacaa
     6541 aagatattgg cgttaatttt ttttcgatac gattatctga ctgatctaga ttaatcacta
     6601 ccaaagaaaa tggatgctct agtttcgtct cataaaggcg ctccatccga gaaacttctt
     6661 ccggatattc ggttcctacg aaaatatcat caggaagctc gaattcttcg attgcttgct
     6721 caatctcttc gggctttgca ctatcaagta cgagccaatt atattttttt tcatctgttt
     6781 ctattacatt cttttcttca tccacaaagt agtatctcaa tgtatacctc ctttttggac
     6841 tttctcgaaa ctattagctg aagaagcagt tctttaattt gatattataa tcgagtactg
     6901 cgatttcatt ttctaatgaa aagctgtcaa gctgtttgtt gaagtccatg ttcaatcgtt
     6961 taccgaaata attttgctct aatttagatg tatagatgtc caataatgga tgctcgattt
     7021 tagtcgcata ttctccaaat tccgaaacaa aaggaatgat ttgaaaaaag ctgccttttg
     7081 caaaagcacc tttgtccctt agaaatggat cggaatcatt gataagcgcg agtgcgattt
     7141 ttgtattttc cccaaccttt tcaattaacg tctctactgc atttttagac attgtaattt
     7201 ccattcctac tccccctctt cagttgttgt cttctattat ttaacaacta catctgtttt
     7261 tggttgaact tttgcatatt ttggatattt agggaattta gctacacaac ggaaagccgt
     7321 catccctgca tatttttcgt aatatttctt cgttggccct tcaaaatcct tgctccacgg
     7381 tttcacatgt cctgaaaaat ggataatgta aggattgttg cgagtttctt cgtattcacg
     7441 ttcacctgct gctgttggat gtttttttgc ttttgccatg atatagcctt gtgcattcca
     7501 acgcggatga agtggtaacc aacggtcatg taagatagca tttaatgcat cttggtcatg
     7561 gaatcgtaat ttttctggat tatgttcgat gaaatctaat actttttgcg taatattttc
     7621 atctaaccat tttttcacat tgatcagcat caaacctgag ttgaaataac gcattgattt
     7681 agctgaaatt ttcatttttt ccagtcgttg atggaaccca gcgtcttcaa cagctgctac
     7741 gattgaatca ccgaagtcaa gacgccataa tttagagata tcttgtaaag cgatcatgtc
     7801 gcaatccata tataagatac gttcaacttc tgtgccgcgg aacaattccg gaatcgcaat
     7861 acgataataa gctgtttctg gaatacgatc gctgattacc acatttgtaa agaattcttt
     7921 attgattttc aagaattcca catctgagtt cattcgtgcg tttttcactg agaagcgtaa
     7981 aagctgttta ctttcaaaat cgatatcatc gtcaatcaca tagaaccgaa caaatttaga
     8041 cgggttgcaa ttatctaata ctgagacaaa taatgcagct aaatgtggta cgaattttgt
     8101 gttacaactt gcgactactg ctatttcttt tttattcatt gatctcgcct actttcgtta
     8161 gagtactgtg tgctaatttt tttaagtata aattggtata cggatgatct ttcaacgtat
     8221 tccaaggttt atcatgtcct gtaaagtgaa cgatcgaagg tttttcattt ccttctttat
     8281 actgacgttc atatttttca tttggtgctg gatgacgttc aaaaatcagc gaagtctgca
     8341 tgttccattt tggatgaagt tgttcccaat cttcataaag tacagcattc aaagcatctt
     8401 ggtcgtgata gatgattcga tcgccatttt cactgaggta atggatagtt ttttctgtaa
     8461 tttctttttt attccattga tcaatgtcaa tcaccatcac tcctgaattg aagtacaagt
     8521 catcggaatc gattcctaat ctttccaaag ctttcacttg gcctggatcg ataatagcgc
     8581 cgattgtttt tccatttaac ggctcatcat atagttttac gatatcgtct agtactaaca
     8641 catctgaatc taagtaaagg actttcttat aatcctcttt agctaataag tttggtaaag
     8701 aaattcttaa gtaagcggta gttgtgatat ggtcactgac taggaagtct tcgtagatat
     8761 ctttttccac tgtcaggaat tggatacttg cattacttga atatttattt accgtttctt
     8821 ccaatccttc tttactatat tctgatagac catcgtcaat cacataaaat tttatgcgtc
     8881 ttgttttatt gcaattttct aatgcagttg cgatcatcac gctcaagtaa ggagcataat
     8941 tttcgtctga agcagttacc acaggaacta cgccgtatct cttctccatc aaaatcactc
     9001 ctttttctaa tctttttcta acctgttcta catctagaat attcctcatt tggttttaaa
     9061 gcaaaatata tgctttgttt tcattgaata ttttaaaatt tttttatcac aaaacgcatc
     9121 ggtaagcgct aactattcgt ttttcatggc tttttattca aaggtattga ccaaaaacta
     9181 ctatatatag ccaccttttt cttaagaatg ttttttgaaa aaattcataa tgacaaagca
     9241 agttagtttc atttttttaa attctcattt aatcttaagg taacaggaaa atagatatgt
     9301 aaaaagaaag taggaagata aaatgaaact agcagcgatt gatttagacg gtaccttgct
     9361 tgatcgaaat ggaaagatac cagaagaaaa tatccaagca cttcggaaat ttgatgaaaa
     9421 tggcggaatc gtaaccgtcg ctaccggaag gaacagcatt tctgctaaag atattttttc
     9481 cgaattagga atcggtggct atttgatttc ttccaatggt tcactgattg ctgaaatgaa
     9541 ggatggaaaa atggcgaaag ttctcagacg ttcaaaaata gatagcgata cactgagaaa
     9601 agccttcttc tatgctcaag aagcgaaaat atccatcatt gctagccgag aaacccaaga
     9661 tgatcagctg acattcgatg aatcaaatct agtcaaagat gacccttatt atgaacattt
     9721 caatttacag aaccattctt ttgatcaaat tttaaatatg ttggaagatc cttctcttag
     9781 ctatcttaaa ttagcattaa cagataagag tgaagaaaaa ctacagaagc tacaaagaga
     9841 tctgcaagca gatggtatcc aatcagtatt ctctgatccg catttcttag aaatcacgcc
     9901 tacaggtatc actaaagctc attcacttgt ttttctggcg gattaccttg gaatcgaatc
     9961 gagtgacatc atggcttttg gcgatcaaga aaacgacatt gctatgttga attacagcgg
    10021 gttgagtatt gcgatgggga atgcacagcg acaagtaaaa gaaattgcaa accaagtaac
    10081 actgacgaat gaagaagctg gtgttgctta tattttaaaa aacttctatt aaaaagaata
    10141 aaatgacgat atgaatgtag gaggaaagaa aatgttagtt attattgata tgcaaaatca
    10201 tattcttgat ccaaacagcg aattttatat tccagagtca gatcaattgg cagaacgtat
    10261 tgctcaaaga ttagctaaag cacgccaaaa taatgaatat attctcttca cacgggatat
    10321 accagttgaa ctgaaggaca aagaagaaga acgagaagat ttgcaattga tccctcttct
    10381 ttctccacaa gaaaatgaac gagtgattaa aaagtattac tttacgattc cacctgaaac
    10441 tttagctgaa atccaggaaa ctttatttga aagcaaagaa gaacaaaaag agatcgaagt
    10501 agtcgggatt gaaacaaact tgtgtgtact ctctaatctg ataggcctgc agagtgcttt
    10561 tcctgaagca gacttttttg ttgatccaat gttagtctcc agtagaaaac acggagaatc
    10621 cgctttagag ctgttgaaag atttcaacgt ctctatcgtc gaatcataaa aatatctatc
    10681 ttcatttacg atatttaaca cacaataaaa aaagcacgca gaactgtttt agtcctacgt
    10741 gctttttcta tcaaagtaga ttggcttttt tatgacacag agtgaaacgg caggctcacg
    10801 acctctttct taaatggtgt tcaatcagct gaccctcggt attagcttaa caaacggcaa
    10861 aatatgagga actatttttc cgtttgttaa gctaatactc aggtcaaaac gctgatttca
    10921 caacctcttc cttaaacggt attcaaaagc ttgcatctgc agtaataaga acagccaagt
    10981 aaaaacataa gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt
    11041 tcacaacctc ttatgtacct tgtaggactc gaacctacga ccggacggtt atgagccgtc
    11101 tgctctaacc aactgagcta aaggtacatc ataaataaaa tttatttttg ttacttttca
    11161 actatagcac gaatgacaaa ctctcacaag tttcaaaata taagtaaaat aatattgtta
    11221 ttctt
//
LOCUS       DAZKCC010000091        11197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000091 DAZKCC010000000
VERSION     DAZKCC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.093"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8VH_002487"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8VH_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9110999.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8VH_002488"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8VH_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9111000.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG8VH_002489"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG8VH_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9111001.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8VH_002490"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8VH_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9111002.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8VH_002491"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8VH_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9111003.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG8VH_002492"
     CDS             complement(6343..7062)
                     /locus_tag="ACG8VH_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP9111004.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG8VH_002493"
     CDS             complement(7059..8372)
                     /locus_tag="ACG8VH_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9111005.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG8VH_002494"
     CDS             complement(8453..8773)
                     /locus_tag="ACG8VH_002494"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9111006.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG8VH_002495"
     CDS             complement(8881..9168)
                     /locus_tag="ACG8VH_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111007.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG8VH_002496"
     CDS             9295..9939
                     /locus_tag="ACG8VH_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP9111008.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG8VH_002497"
     CDS             complement(9998..10705)
                     /locus_tag="ACG8VH_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9111009.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG8VH_002498"
     CDS             complement(10925..11095)
                     /locus_tag="ACG8VH_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111010.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DAZKCC010000092        10936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000092 DAZKCC010000000
VERSION     DAZKCC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.094"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8VH_002499"
     CDS             <1..154
                     /locus_tag="ACG8VH_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP9111011.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8VH_002500"
     CDS             complement(208..486)
                     /locus_tag="ACG8VH_002500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111012.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8VH_002501"
     CDS             complement(601..1218)
                     /locus_tag="ACG8VH_002501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111013.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8VH_002502"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8VH_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9111014.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG8VH_002503"
     CDS             2780..3196
                     /locus_tag="ACG8VH_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9111015.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG8VH_002504"
     CDS             3193..3663
                     /locus_tag="ACG8VH_002504"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9111016.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG8VH_002505"
     CDS             3677..4450
                     /locus_tag="ACG8VH_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9111017.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG8VH_002506"
     CDS             4464..5276
                     /locus_tag="ACG8VH_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9111018.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG8VH_002507"
     CDS             5330..6295
                     /locus_tag="ACG8VH_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9111019.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG8VH_002508"
     CDS             6431..9154
                     /locus_tag="ACG8VH_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9111020.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG8VH_002509"
     CDS             9167..9799
                     /locus_tag="ACG8VH_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9111021.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG8VH_002510"
     CDS             10007..10231
                     /locus_tag="ACG8VH_002510"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9111022.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG8VH_002511"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG8VH_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFP9111023.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG8VH_002512"
     CDS             10556..10825
                     /locus_tag="ACG8VH_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9111024.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3625 a         1742 c         2137 g         3432 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct ttttta
//
LOCUS       DAZKCC010000093        10865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000093 DAZKCC010000000
VERSION     DAZKCC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.095"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1557)
                     /locus_tag="ACG8VH_002513"
     CDS             complement(46..1557)
                     /locus_tag="ACG8VH_002513"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9111025.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG8VH_002514"
     CDS             complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG8VH_002514"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9111026.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="ACG8VH_002515"
     CDS             complement(3384..4460)
                     /locus_tag="ACG8VH_002515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9111027.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="ACG8VH_002516"
     CDS             4656..6401
                     /locus_tag="ACG8VH_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP9111028.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="ACG8VH_002517"
     CDS             complement(6681..7118)
                     /locus_tag="ACG8VH_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9111029.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="ACG8VH_002518"
     CDS             complement(7102..8475)
                     /locus_tag="ACG8VH_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111030.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="ACG8VH_002519"
     CDS             complement(8797..9285)
                     /locus_tag="ACG8VH_002519"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9111031.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="ACG8VH_002520"
     CDS             complement(9307..9909)
                     /locus_tag="ACG8VH_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9111032.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(9978..>10865)
                     /locus_tag="ACG8VH_002521"
     CDS             complement(9978..>10865)
                     /locus_tag="ACG8VH_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFP9111033.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         3166 a         2269 c         1918 g         3512 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc cttatgaact
     9961 gaaccccaaa agttgaacta tttaatggac tgtttccgat attctactgg agatagtcca
    10021 tttaacttta gttttatcct tttgttatta taccacctaa tatactcatt taatttactt
    10081 tgaaatatct caattgatcg gaatttttct cgataaaaaa actctgattt aagcacacca
    10141 aaaaaatttt ctattacaga attatctaag caatttcctt ttctagacat gctttgaata
    10201 atgttattct cttttaattt tttttgatat tgtggcatct gatactgcca tccttgatct
    10261 gaatgtagaa tcagtgaacc ctcagttccc ttttttttaa ttgcttgttg aagcatttct
    10321 tcaatcagtt tatatgttgg actagttgat atactataac taattatttc tccgttaaat
    10381 aaatcaagta taggagatag atagattttt cttcctttta tcttgaattc agtgacgtct
    10441 gtcacccatt ttttattggg tgtatctact gaaaaatttc gttttaacac attcttggca
    10501 atttttccta ctgttccttt ataagattta tatcgcttta ttcggatttg acaggtaagt
    10561 cccatttggg acattaattt tctaactgtt ttatgattga ttgtatatcc tttcatcttt
    10621 aacgctaaag tgactctacg ataaccataa gagtttcttg attcttttac aatcgctgta
    10681 atttcttgct ttatcttgct atatttatct ggcttatcta atttttttac ccagtaataa
    10741 taagttgact tcgctaattg tgcaattgaa agtaatagat tcaatttaaa ttcttttttg
    10801 agctgaagga ttgttttaac cttgatttct tcttgctcaa atcttgttct tgaatcaagg
    10861 tttct
//
LOCUS       DAZKCC010000094        10745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000094 DAZKCC010000000
VERSION     DAZKCC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.096"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..972
                     /gene="thiD"
                     /locus_tag="ACG8VH_002522"
     CDS             157..972
                     /gene="thiD"
                     /locus_tag="ACG8VH_002522"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9111034.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1301..2752)
                     /gene="cls"
                     /locus_tag="ACG8VH_002523"
     CDS             complement(1301..2752)
                     /gene="cls"
                     /locus_tag="ACG8VH_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9111035.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3093..5759
                     /locus_tag="ACG8VH_002524"
     CDS             3093..5759
                     /locus_tag="ACG8VH_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9111036.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6123..6875
                     /locus_tag="ACG8VH_002525"
     CDS             6123..6875
                     /locus_tag="ACG8VH_002525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9111037.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6897..8051)
                     /locus_tag="ACG8VH_002526"
     CDS             complement(6897..8051)
                     /locus_tag="ACG8VH_002526"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP9111038.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8236..10677
                     /locus_tag="ACG8VH_002527"
     CDS             8236..10677
                     /locus_tag="ACG8VH_002527"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP9111039.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3501 a         1912 c         2305 g         3027 t
ORIGIN      
        1 actattttct gatttattgc aaatgttttt tcaaataaat ttgattacca agaaaaggta
       61 gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta acagtattat
      121 gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta tgtacaagca
      181 atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga tatgcataca
      241 ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc aggtaattct
      301 tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga atttgaactg
      361 attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga ttcaaagttg
      421 attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt cgtggatccc
      481 gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca aagcttgaaa
      541 gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc agaaaagctg
      601 actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga actgcaagat
      661 atgggtgcaa aaaacgttat gatcaaagga catcatgcta aaaatgcgca gcaagaaaca
      721 gtcaatgact acgtattgtt cgaatcagga aaatcatttt ggttaaatgc accattttat
      781 cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc agctgagtta
      841 gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt aaaccgtgca
      901 attcgtgaag aaataaatgt agggcacaaa tatggcccca tcaatcattg ggcagctggt
      961 gaattagagt aaaagaaaga agttgtgaaa aagttgccct gcatcaagta ataagaacag
     1021 ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg cagatgcaag
     1081 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tccagctccg agtcacaagg
     1141 aacaagttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
     1201 tggcaggaga acaccgtttg ttcggaagaa gggactgtga caagattttt gtcacagtcc
     1261 ctttaatctt agtatatttt tctctacttg aataaaaaaa ttataggatt ggagaaaata
     1321 atcgagaaat ttgttgcttg aataatagcc agttagacat ttccttgact gtttcagttg
     1381 tcatggatga acaatgtgtc tcatcttctt tgaactgagt agcaagttct tctaaaaact
     1441 caggatcgta aatgacagca cttgtttcaa aattcaatcg gtagctacga atatcttgat
     1501 ttgctgaacc taccatgcag atttcatcat ccattatcaa tgtttttgca tggaggaagc
     1561 cgttctcata gacaaggatt tgtacatttt ccttgatcag ctgccgagca taatattgtg
     1621 ttgctcgata aataaaagga tgatccggtt tgttgggaat catgattttc acatctacac
     1681 cagaggcagt tgcgattttt aaagcagcaa tgacactttc atcaggaaca aggtaaggtg
     1741 tctgtatcca aacacgtttt ttagcagaag taatcaattt gataaatgaa agcttgattt
     1801 gttccctttc gttattcggt ccgcttgaaa caagctggat ggatgtatca gcaagcttag
     1861 attcatctcg atctgctttt ttaaagaaat aattcaaatg atagccgacc tttttttctt
     1921 caggtaccga gacgttccag tctgttaaga agcgaagctg gagcaaagaa gaagcggcac
     1981 cgaaaatccg tatatgcgta tcgcgccaat agccgaattt ttttgttgta tttacatatt
     2041 gattggcaat attgaaacca ccggtataac taatctttcc atcgatcaca acgatttttc
     2101 ggtgatcatg gtaattcaat cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat
     2161 ggacatgtcc accatttttg attagacgat tgaaatcttt tgcttttgtg ccatgagagc
     2221 caaatgcatc atataatatt cgaacttcca cgccttctgc ggctttttct tctaaaacat
     2281 gtaagacttg ctggccgata ttatctgtca caaatgcgta atattcgata tggatcgaat
     2341 gtttggcttt ttttagatct tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa
     2401 gagtgacaga attcattctt gtcagcggca tacgatttaa tgaagagaag aagtcaacaa
     2461 attgctgttt gtctttatcg cccatgtcag gatcgtaatg ttcaatggta tctcctctaa
     2521 aagactgaaa gttttctaat tctttcaagt cactttgtcg gagatagaat tttttcttat
     2581 ccgttaatcc acgtccgaaa aataaatata atacaaagcc caccccagga agggcaaaca
     2641 atactaagag ccatgcccaa atggctgcta catctcgggg tttgatcaaa atagccacca
     2701 aagcaataag tgcatttaat agataaaggg cggttataat actagatacc acaaaaatgt
     2761 tcctccttac atacaataac attagtatag catgatagaa gaaagaagcc taccaaggtg
     2821 aatatacttt tcactccttt catgaaaatg taggtaaatc gggctaaagt agttgctttt
     2881 tgtaggttaa taaggtagga tattagtaca gttgaatatc tccttagggg agtaaccggc
     2941 aacgtttgtt gcgataatat caacagtaag ttaaccaaat ggttttctgg tattgtatga
     3001 aatggtgaga cctaagacaa atgcgtattt gtcttaggtc tctttttttt cagacaggac
     3061 tagggccaag atcaaaaaat gggggatgaa atttggaaaa caaggaacaa gcacaacaag
     3121 cggtctataa agaaacgatc gactcgttga tgcagcaaac gcagagctca accgatggat
     3181 tgagcaccac gcaagcgaag gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa
     3241 agccgaagaa gccaacttgg aaattgttct tagaaacttt caaagatgca atggttatcg
     3301 tattgctgat tgtcgcactg attcagatgg tcatgggtga ctacatcgaa ttcttagtta
     3361 tttttgcagt attgatgttg aattctataa tcagtgttgt ccaaacaaaa aaagcagaag
     3421 gttctttaga agcgttgaaa aacctttcag cgcccgatgc tcgagtgatt cgtgatggta
     3481 aggaaaaaac gattccggca aacgaacttg tggtaggaga tatcgtgttg ttagacgcag
     3541 gcgattatgt acctgctgat ggacgtttac tagatgcggg atcattaaaa gtagatgaag
     3601 gtatgctgac aggagagtcg acttctgcag aaaaagaagt aacggatatc acaagtacgg
     3661 taccaatcgg agatcgtaca aatatggtat tcagtggaac gatcgtaact tacggacgcg
     3721 gtaaattctt agtgacttca actgggaata atacggagat cggacaagtt gctggccttt
     3781 tggaatcaac aactgagcaa gtcactccgt tacaacgagg tctggataaa ttcagtaaaa
     3841 aactaagttt ggctattttg gcattgtctt tattgatttt aggtatccaa ctgtttcgta
     3901 tttatcttgg agaaggtaca ggagatatga ctgcagatat cgtcagcgca gtgatgtttg
     3961 ccgtagccgt agccgtagct gctattccag aagcattaca gtctatcgta acaatcgtgt
     4021 tatctttggg aacgaataaa atggcaaaac gtcatgccat tattcggaag cttccagcag
     4081 ttgaaacact tggttcgaca agtatcatct gtacagataa gactggtaca ttgacacaaa
     4141 acaaaatgac agttgttgac tactttttag gtaatggcga tactggggat ttcacaaatg
     4201 acccttcaaa atggacagct gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg
     4261 atgcatcgat cagtgaagac ggtactaaat tgggcgaccc tacggaagta gcgtttatcg
     4321 actttagtga aaaattaaac cagccttatc aggaaatccg aaacaactac ccaagacaag
     4381 cagaacttcc ttttgactca gatcgaaaat taatgtctac tggacacaca attgataaca
     4441 aaaattactt gtttgttaaa ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat
     4501 tgctaaatgg tgaagtcgtt ccgatgacgg atgaattgat gaaaaaattc caagagcaaa
     4561 atgaagcttt ttcaacacgc gcattacgtg tactagcttt tgcttaccgt ccacttgata
     4621 attttgattt atcatttgaa gatgaagacg atttgatcct tgttggtttg cttgctatga
     4681 tcgatcctcc acgtgctgaa gtgactcagg cggtaacaga agcaagaagt gcaggaatca
     4741 aaacaatcat gattactggt gaccacaaaa caacagctgt tgctatcgca aaacaaatcg
     4801 ggatttttga agaaggaaac ctggctttga ccgggacgga actagatgaa ttgacagaag
     4861 atcaactaaa cgaagtttta gaaaaagtca ctgtttatgc acgtgtttca cctgagaata
     4921 aaattcgtat cgttcgtgct tggcaaaata aaggtcatat ctcagctatg acaggtgatg
     4981 gtgtaaatga cgcaccagca ttgaaacaag cagacatcgg aattgctatg ggaacgggta
     5041 cagatgtggc aaaagatgca gcagcgatgg tattgacgga tgataacttt gcttctattg
     5101 tcagcgcagt agaagtagga cgtaatgtgt ttgataatat caagaaagct atttcttatc
     5161 tatttgcagg aaatttaggt gcgatcattg cgatcatctt tgccttagtt gctgactggt
     5221 ccaatccatt tacagcatta caactgttgt tcatcaattt agtcaatgat tcactgccag
     5281 caattgcatt aggtacagaa aaagctgaac ctgctacgat gaaccgcccg ccaagagatc
     5341 caaattcagg aatctttacg gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga
     5401 ttgggattgc tgttatcttt gctcaatggc ttggcatgca gcattcacct gaattcggtg
     5461 tagccatgtc attttcaaca ttgatttggt gtcggacatt gcaaacattc ccagcacgtt
     5521 caaacacaca aacagctatc caagcagggt tcttctctaa cagaagtgtc ttactagctg
     5581 tagttgtatg tagcgcgctt tactgcttaa caatgatccc aggactgaaa gaagtcttct
     5641 ctatcccatc agcatttgga ttgacagaag tctttacatg tatgggaatc gcacttgtga
     5701 caattgtttt gatggaaatc actaaattga tcattgtacg agtacaagga aataaataaa
     5761 acgaatcaac gaggttgtga gcctgccgtt taattctgag tcacaaggtg caatttcaac
     5821 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaagagttg gcaggagaac
     5881 accgtttaag gaagaagttg taaaatcagc attttgagct aatactgagg gtcatctgat
     5941 tggacactgt ttattcggaa agagggactt tgacacgact ttggtcacag tccctttttt
     6001 tatttatgat agacggaaag ccaatcattt tattattcat caaaagagat agaatagata
     6061 aaagaggaga atgatttatt ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc
     6121 aaatgaatga aaaagaatgg gatgaatttg ctgaagaata ttatgaaatc cagcaagaat
     6181 cgcaagtaac tattgctgaa gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg
     6241 ctgaaacagc agcagacgtt gctggaggat ctggcagatt tctttcctta gcaaaagaaa
     6301 ttaatcgcta tgatttgatc gatttttctt caggaatgct agaaaatgca aaaaaagaag
     6361 cagaaaaaaa tgagttgcca aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc
     6421 aatcaaatga gacatatgaa atggttttta cagcagcaaa tccagcattg gtacatctta
     6481 ttcaattaga aaaattgatg aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg
     6541 aatcaatcga tgatgttttt tatcctatcg aaaagcaact aggaatgata gaagaagatc
     6601 ctaatacatc gccagttgta atggatcaat ttgaaacata cttaattgat gaaggattca
     6661 aagtgaagaa aaaagatttt acttatttta cacaggaatc aattactgaa gaattgatga
     6721 agctatacta tgacgcgtac gcaaaagatc ctgttgtgac caggtacatt cgcaaactat
     6781 tcagtgaaaa acgagaaatc cttagtacga ctaagctgac ctatagactg ctcctttggt
     6841 ccgcagataa aaaaagaacc tttcacgact cgtgaagggt tctttttatg attttattat
     6901 attttgtagt aacgaatatc attttcgatc tttgtaatcg aaaaaggtac atctgaagta
     6961 tattctgcac attctccgtg ttcaggaagc gtctcactga aaatcgtttc atattcgtca
     7021 acagtgatcc gtgttcgaga atctagcatc tcttgatgag attgtgcgaa gagatattct
     7081 tgatagtttt cttccagata acctgtaaaa aattcactta ctgcaccaga accgtagctg
     7141 aatagaccga tgcgatctcc tggctgtagt gatttcgaat tttccagtag agaagttagc
     7201 cccaggtata atgaaccagt gtaaagatta ccgattcgtc ggctgtaacg gatgctttct
     7261 tcatagcgag ccataagacg ttcctgattt tcttcgtctg tttggtctaa tacgctttgc
     7321 aatgcctttt ttcccatctt agtatacgga atatggaaag caatcgcctg ataatcttcg
     7381 agtcctcgac ccgacaattc tttatgtcga ttccaaactt tttggaatga ttcgatatac
     7441 gtagaattag ataaaggacc atcaacaaca ggaaattcgc tataatctgg acgccagaaa
     7501 tcatagatat cttctgtcag aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt
     7561 tgagtgatca tcatcgcaac agcaccgaca ccttgcgtca cttcaccacc gcttgccaag
     7621 ctgtaacgag caatatcact tgctatgact agtacttttc gttctggatg atttttgaca
     7681 tattctttcg ccatgtgcaa agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa
     7741 gaacgagcaa aagattggat tttcagtaaa tggtggataa ttaccgcact cgcttttgaa
     7801 tgatcaatcc cggattctgt tccgacgatg accatgtcga ttagctcacg gtctttttct
     7861 gttacgattt tgctggcagc atttgctcct agtgtaatga tgtcttcatt tgcacgattg
     7921 actgccattt ggtcttgacc aatccctata tggtattttg caggatcatc cccacggctt
     7981 tctgccaact ctgtcatatc taaatataaa ttaggaataa aaaaggaaag acgatcaatc
     8041 cctattttca tttcaacacc tctttactta tgataaaaaa cttactttac taaggtatca
     8101 taaaaagatt aaaagaaata taaaaaagag aatatttaag gattgttaaa atttaatctt
     8161 agaaaaaaat aactggaaca agagctattt gtttttacag caaagaatgg tatatttttg
     8221 taggaggtgg cttacttgaa agaagtcgtt atgatagatg ctgcaagaac gccgatagga
     8281 aagtatcgcg gaagtttaag tccatttact gcagttgaat taggcacact tgtgactaaa
     8341 ggattattag ataaaacaaa actgaaaaaa gataaaatcg atcaagtaat atttggaaat
     8401 gtcctgcaag ctggtaatgg tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc
     8461 cctgtagatg tcccagcaat gacgatcaat gaagtttgcg gatctggtat gaaagcagtg
     8521 atcttagcac gccagctgat ccaattaggt gaagcagaac ttgtgatagc cggcggaaca
     8581 gagagtatgt ctcaagcacc tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt
     8641 gaaccaattt ccagtatggt caacgacgga ttgactgacg cattttcaaa tgcacatatg
     8701 ggattaaccg cagagaatgt tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc
     8761 tatgccttgt cgtcccagtt gaaagcagca catgctgtcg aagccggtgt attttctgag
     8821 gagatcatcc cagtcaagat ttctgatgaa gacgtgttat ctgaggatga agcagttcgt
     8881 ggaaatagta cattggaaaa actgggcacg ttacgtacag tattctcaga agaaggaact
     8941 gtaacagcag gaaatgcttc cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc
     9001 aaagaatacg cagaaaataa taatctgcct tatttagcaa ccatcaaaga agtagcggag
     9061 gtcggtattg acccaagtat catgggaatc gctccaatca aagccattca aaaattgaca
     9121 gatagatcag gaatgaattt atcaacaatc gatctttttg agatcaatga agcatttgca
     9181 gcttcttcta tcgtagttag ccaagaactg cagttagatg aagaaaaggt caatatatat
     9241 ggaggagcaa tcgcattagg ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca
     9301 ttagcttacg gattgcttcg tgaacagaag agatacggta ttgcttcatt atgtattggt
     9361 ggaggtttag gcttggcagt cttgttagaa gcgaatatgg agcaaacaca taaggatgtg
     9421 caaaaaaaaa agttttatca gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa
     9481 aatgtattga cacaagaaac tgcgttgatt tttcaggaac aaacactttc tgaagagctc
     9541 tccgatcaca tgatcgaaaa ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag
     9601 aattttcaaa tcaatggcaa gaaaaaatgg attccgatgg caacagagga accatctgtc
     9661 attgcagctg caagtaatgg tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag
     9721 cggctgatgc gagggcaaat cgttctatca ggaaaatcgg agtatcaagc agtgatcaat
     9781 gcagttaatc atcgaaaaga agagctgatt ctttgtgcaa atgaaagtta tccttcaatc
     9841 gtgaaacgcg ggggcggtgt tcaagatatc tctactcggg aattcatggg gagtttccat
     9901 gcatacttat ctattgattt tttagtggat gtaaaagatg caatgggcgc taacatgatc
     9961 aactcgattc ttgagagtgt ggcaaacaag ttacgggagt ggttccctga agaagagatt
    10021 ctattcagta ttttaagtaa ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc
    10081 ccattcgaac gcttagggag aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa
    10141 caggcagggg aatatgcgaa gcttgaccct taccgtgcag caacgcacaa caaagggatc
    10201 atgaatggta tagaagctgt agtggcagct acaggaaatg atacacgtgc ggttagcgct
    10261 tcgatccacg cctacgcggc aagaaatgga ttataccaag gattaacaga ctggcagatc
    10321 aaaggggata aactagttgg aaaactaacg gtgcctttgg ctgttgccac agtaggcgga
    10381 gcttccaata tcctgccaaa agcaaaagca tctttagcta tgttagatat tgattcagcc
    10441 aaggaactag cacaagtaat tgctgcagta gggttagctc aaaacctggc tgctttgcgt
    10501 gcattagtta cagaaggaat ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg
    10561 atctcaattg gggctattgg tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca
    10621 gaaaagatga accagcagac agctatacaa atattggaaa aaatccggga aaaataaaaa
    10681 agcaatatct ttagaagaat aaagcaagat ttacagttat tttatttact gcagatcttg
    10741 ctttt
//
LOCUS       DAZKCC010000095        10668 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000095 DAZKCC010000000
VERSION     DAZKCC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.097"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..311
                     /locus_tag="ACG8VH_002528"
     CDS             <1..311
                     /locus_tag="ACG8VH_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HFP9111040.1"
                     /translation="AEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRL
                     QNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD
                     "
     gene            346..1113
                     /locus_tag="ACG8VH_002529"
     CDS             346..1113
                     /locus_tag="ACG8VH_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9111041.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1305..1703
                     /locus_tag="ACG8VH_002530"
     CDS             1305..1703
                     /locus_tag="ACG8VH_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9111042.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2010..2384)
                     /locus_tag="ACG8VH_002531"
     CDS             complement(2010..2384)
                     /locus_tag="ACG8VH_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111043.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2406..2756)
                     /locus_tag="ACG8VH_002532"
     CDS             complement(2406..2756)
                     /locus_tag="ACG8VH_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111044.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(2773..3168)
                     /locus_tag="ACG8VH_002533"
     CDS             complement(2773..3168)
                     /locus_tag="ACG8VH_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9111045.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3235..3420)
                     /locus_tag="ACG8VH_002534"
     CDS             complement(3235..3420)
                     /locus_tag="ACG8VH_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9111046.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3502..3756)
                     /locus_tag="ACG8VH_002535"
     CDS             complement(3502..3756)
                     /locus_tag="ACG8VH_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111047.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(3778..4023)
                     /locus_tag="ACG8VH_002536"
     CDS             complement(3778..4023)
                     /locus_tag="ACG8VH_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111048.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4030..4314)
                     /locus_tag="ACG8VH_002537"
     CDS             complement(4030..4314)
                     /locus_tag="ACG8VH_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111049.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4417..4599)
                     /locus_tag="ACG8VH_002538"
     CDS             complement(4417..4599)
                     /locus_tag="ACG8VH_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9111050.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(4805..5290)
                     /locus_tag="ACG8VH_002539"
     CDS             complement(4805..5290)
                     /locus_tag="ACG8VH_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111051.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVDTKVTKIYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            complement(5457..5723)
                     /locus_tag="ACG8VH_002540"
     CDS             complement(5457..5723)
                     /locus_tag="ACG8VH_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9111052.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5725..5946)
                     /gene="relB"
                     /locus_tag="ACG8VH_002541"
     CDS             complement(5725..5946)
                     /gene="relB"
                     /locus_tag="ACG8VH_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFP9111053.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            6172..6990
                     /locus_tag="ACG8VH_002542"
     CDS             6172..6990
                     /locus_tag="ACG8VH_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9111054.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            7003..7290
                     /locus_tag="ACG8VH_002543"
     CDS             7003..7290
                     /locus_tag="ACG8VH_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111055.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7472..7825)
                     /locus_tag="ACG8VH_002544"
     CDS             complement(7472..7825)
                     /locus_tag="ACG8VH_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111056.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(7845..8900)
                     /locus_tag="ACG8VH_002545"
     CDS             complement(7845..8900)
                     /locus_tag="ACG8VH_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111057.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            9116..9514
                     /locus_tag="ACG8VH_002546"
     CDS             9116..9514
                     /locus_tag="ACG8VH_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111058.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            9517..9690
                     /locus_tag="ACG8VH_002547"
     CDS             9517..9690
                     /locus_tag="ACG8VH_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111059.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3542 a         1636 c         1819 g         3671 t
ORIGIN      
        1 gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg aatagcaaat
       61 acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc gatcttaaac
      121 ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc aaagatagag
      181 gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag gattacacaa
      241 taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct tatgtgaacc
      301 tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa aaaagatgaa
      361 ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga tttaaagtcg
      421 tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa gaaaacaggc
      481 gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca aaatcttgaa
      541 acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga aatttcgaat
      601 ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat tcattgggat
      661 ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt aattgtctca
      721 atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca aaaaaaatat
      781 agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat cgccaaaaat
      841 aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat agttaaaatt
      901 cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga tagacgtgtt
      961 ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga ttttgctaaa
     1021 gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag tgagtttgat
     1081 gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac tcgtttgaag
     1141 atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta atctccttta
     1201 gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct tttttttgaa
     1261 acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa tacttttttt
     1321 gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat tttccagact
     1381 tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa gtattggaag
     1441 gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg cctgttcaag
     1501 agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta aaagctaata
     1561 cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg actgtggaac
     1621 actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct ttgaatgatt
     1681 tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa aaacagcttg
     1741 ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa aagctctcag
     1801 aagccttctg agagctttta aattgctatg tattgtactg catctgctca aattgatttt
     1861 cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata tgtattgagt
     1921 aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat gtgttaggat
     1981 aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt tttatcggta
     2041 ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat ccctgcatcg
     2101 ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat caaaatggtg
     2161 tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga cttataaaaa
     2221 agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta tttccaaaga
     2281 tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc tgctaattga
     2341 tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct cttctcctgt
     2401 ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc ggtttgaaat
     2461 tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga ttcacttaat
     2521 gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt atagcagaat
     2581 tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa gttattatcc
     2641 tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc agttgtatct
     2701 tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc tttcataggt
     2761 tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa gtagttgaga
     2821 gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac taacattttt
     2881 tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt ggatgattac
     2941 taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag gaaattctgc
     3001 atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat atgcttcttc
     3061 aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt tataagcttc
     3121 tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt cctcgctcct
     3181 tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag gctgttaaag
     3241 ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat tgtggatcgg
     3301 aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc cagtttgtcc
     3361 agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg tcttaggcat
     3421 agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat caatagaaaa
     3481 agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag caaattgaat
     3541 gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat aggtatctaa
     3601 gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt tctccaagtc
     3661 ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta tgaaaacata
     3721 ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt tcttgtacta
     3781 agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata ggacgtgacc
     3841 gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa ggctagtacc
     3901 tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg gatcaatgcg
     3961 agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt ccaaagtcca
     4021 cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt caatagtcac
     4081 ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca agttcccgat
     4141 gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca cttgccgact
     4201 gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg cattaattgt
     4261 ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta gcatcttgtc
     4321 actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc ggtgtgtata
     4381 atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa acgataaagt
     4441 aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg ctttgcttgt
     4501 gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc cagtaaagaa
     4561 taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc cttctgtaat
     4621 attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat ggtgatgttt
     4681 ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt acctaatacg
     4741 tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt tatgtattta
     4801 agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga gtgagtgatc
     4861 catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca gcgacatacg
     4921 atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt tttggtttat
     4981 tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga tcaccgacaa
     5041 agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca tttaagaagt
     5101 agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag gttccttctg
     5161 catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg atttccaaac
     5221 catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa taatcaccat
     5281 accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca cttatattat
     5341 acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa gtgggatcgt
     5401 aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct tgtctgttac
     5461 ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc atgctcgcta
     5521 atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga tttattagct
     5581 gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt ctcgatccag
     5641 tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg agcttcacgt
     5701 tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat cttcgtggga
     5761 aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg caaagtctac
     5821 taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt tatctccgtt
     5881 gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa tacttattac
     5941 tggcatagtt tatcaactcc ttttcaatta cattgtacta cgattgtatg acaaatgcaa
     6001 tagttatata tttgaatata tacataaaca taaacatata catatatatg tttatgttgt
     6061 agaatataaa agcaaatata aatataaaca cgtttatgta tataaacata tacataaacg
     6121 tgtttatagt gtataatgga gttacaaaag gttaataagg gaggtttaat catggaaaag
     6181 aagttgtcaa tcattactgg aaacattgaa aaaggtggaa caggtaaaac aacaacaatc
     6241 tacaatctag gtggatatgc tgtcgaaaat ttaggtatga aggtgctgta tattgatgaa
     6301 gataagtcgc aaaacctatc aaaacgattt cctgatgctt tcagtaaagc gaaaagagag
     6361 aatacaatca atcatttata tcttcatgga tcagctgaac ctttagctat aaatgagaac
     6421 atggatattt tagtagcagg tgaagattta aaacaagttg agatgcaaat gcgtgatatg
     6481 cctaacaaca gaaccctact gtttagatgg atcgttgtta actatgaaca gcttaaagag
     6541 aagtatgatc taattctgat tgatactcat aatgatgcag gtctactagc tcaaaatgct
     6601 tgggctgtat ctgatcttgt cgtaggcatt tctgatccat ctaaagatgg ttttgaagct
     6661 ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac agttgattga tattaagact
     6721 ggtgaaacac tgatggaaac taaatacgct acgatcggta gcaggatcaa acataatact
     6781 aattcaagtc gtgagttcct agaattggta ccaacgttat ctaattatct tggatatgta
     6841 caagagaagg aattgcttaa tgcaactagc ttagaaggta agaatattgc tgagttcgct
     6901 aaggataaga atatctatcg taagaataag gatttctttg aaagcacgtt tacgctatac
     6961 aacaaaataa ttgatacggc acaagaataa ggagtgattg aagtgaaaga aaatagattt
     7021 aaaaaattcg ctgaagctga agtagaagaa gttcctgaag tggttgaagc agttgaagaa
     7081 cctaaacaaa cacctaaaga aacaacaagt tctttggtag ctaaactaaa aagtgatagt
     7141 tctgaaaaga agaaaaatta tcaattcacg cttcaaccat cacaaagaaa gaaattatct
     7201 aaaatcagcg aaaagttagg aattcgatca gattcagctt tacttggtga aatgatagat
     7261 aacttgtatg atgaagtttt tggtgagtga tcagtttaaa gctgatcact ttttttattg
     7321 gaataagcgg ttaggtatag cttaactaaa tttcgtatac gtgtttatgg atatatacgt
     7381 atacatgttt atatacatgt atacgtatat gtttatgttt gtatttgtac ataataaaaa
     7441 ggctaggtat tgtaacctcg cctttttatt tttaaagtaa gtctttgtta cttggtgcat
     7501 tgaacccttt tagcattggc ttttctttct ttgctgaatg atcggatgta accgttgaag
     7561 gatgctcttg ttgaataggg ttatgatcag ttggtttctt ctttacttct gctactttgc
     7621 tatcgtttct atcgttgcct gcttcaataa ttgtaacttc attgttcttt acacgccgat
     7681 ataggttcaa agcttttttg atcgtctgag agtatttaac gcctagtgtt tcatattcgg
     7741 attcaagaaa ctctgctaaa ggtaactcgc taaccttttc tctattgatt ataactacat
     7801 aacgctctga ttttccctta ttcatgttga tctccttcgc tgttttattg agttaaacca
     7861 aacttgtaaa agccttttac attagaaaat tcactgtctt caacaaacaa ggtatgcgga
     7921 attttatctt ttacaagttg tttgtcgata atagaagctc caccaccagt aaagacaact
     7981 ttatctaagg ttgtgaagtt tttgaatgta gctaaaactt ctgcaacaac catattggtg
     8041 tatgttttta tttctctttc aactacttct gaaatatcaa aactttcatt tccgcttggc
     8101 ttaaatagat atacgccttt ttctttgcct tctcggataa tcttttcaag ttggaaagca
     8161 ttaggactga tcggaaggtc aagcatattt acgatacgat tatataaagt aaaaatgccg
     8221 gtatcctttt gttcacgatt gttttcgtca atttcaaaat tttggacaac atcaaaaagt
     8281 ttcgtgccac caccaatatc aacaaaaccg atcttctgac tgatcaatgt ttcatcttgt
     8341 acactaccat catcagcaag taggaggtta tatagtgttc caatcggctg tggcagtacc
     8401 caaacggtat ctacgtttat attataagcc ttaccgtcaa cttcaactga atgttgtcgt
     8461 gtggcttgtt gcatcacgta tttgacgatt gtttcggtat agtcggatga aggcagacct
     8521 aaaactagat taacggttaa aacgtcatca gattgatctt ctaaacgtgc taggttagct
     8581 aaagagaaag ataggatatt tttgtaggtt tcctgcttgt atctgctgat cccaaaacct
     8641 agtgattcta tcacgtagtc atcttttcct agctcgtgta tatccttccc aaaataatag
     8701 ctttcagtag tgtttactag gctatattca gaaacgttta gtgatcgttt taatgaggaa
     8761 cctatattag aaaaacgtcc tgaattaatg atatttttgt tcaaaatacg agagggaagt
     8821 gagagtggag aaacgtgtgt actcatttct tttactgatt tattccctaa gtcaacggaa
     8881 aacaataatg gttctttcat ggtatgacct ctttctgttt gtgtttatgg tatttcggta
     8941 cgaaaatagt ttacacctat ttactattag ttgcaactgt ttataagaaa aaaatgcaat
     9001 cataaaagtc taattaatta taataaagtg taaataaaaa ggtgtaaata aatgcttgca
     9061 aatttatctt attatgatat tataaaatca taaattatat taaggagtga tcctgatgcg
     9121 attgagaaga aataagaatt acactaccat tattttagaa gattttactg atcaagaatt
     9181 acttattgtc ggtaaaacaa aagcagattt tatcgaaaag ggaaatgctt caatagtaaa
     9241 agcttcaaag gttgctgtcg aaaaatattt tgattcttta gaagaaagtg ctaaggaaat
     9301 ttttaatgga gagaaagctg gtagctatga gttagacggt attagattta gcttgatccc
     9361 aacaactgaa aatattattc cgaagaaatt atattatatc ttttcatctg aaaaacatac
     9421 ttcaatgaag ccttgtattt ttgattggca atggttgatt gatgctgtat tggatgtaaa
     9481 atgttcaaaa tacaatatta ctttggggtg ttaattatga gtatgctagt tagaaaatta
     9541 ccattatctg taagagaatt tctattttac ttggtctgct taatcgcatc agggtttaag
     9601 ctgatcgaac atatttttat ctttgcttat gtattagcaa tgatcgcatt tttatctggc
     9661 ggttggttaa ttatttattt gttaagctaa gttttagatc aaaaataatt agtaaattaa
     9721 acgataaaat aaccgttaat aaatgtaatg aaaggacaac gcctaacaat cagcttgtaa
     9781 taagattata aaataacata atatcaaaaa tagtgctttt taaaggacaa attaagtgta
     9841 tcaacataaa cgtgtatatg tatatgttta tgttgatact ttttttgtct taatttttta
     9901 tttttaaaat tctatttact aatgctgtat agatagtgat cagaaaaaac aaaatagcga
     9961 gattaagcta tttattagtt catatattat attcagatta atatgatcca gtattaatct
    10021 gaaggcaaaa ccaatatatt tctacggact cttgtaaaac tatttaaatt ttcctgctaa
    10081 taaaataacg aaaatagaat atttagcttt tttataccat tttacgataa catgatttca
    10141 tttgtaatat taaaacattt aaatttttaa ttattctttc aaattagatc aattaatatc
    10201 cttataaaag agcataataa atggttttta catcgttatt tattgatatt aatctcatcc
    10261 aaaaaaaatg aaaacgcata cttttatggg tgattgaatg tgattttaat acatatacca
    10321 attaaaagaa aactttttag atttttactt acaaatttat aaaaaaacaa gataattgat
    10381 actttgattt tgcgaacatt ttatcttgcc gcctaattca gctttaatat catgattata
    10441 tcagtaacaa atcgcttttt tagctaattt aagcatcaaa taattattct taatcaggga
    10501 aaaaataact aataaatgga ttttgggaat aggtaaaaac ttgatcggtt tctggctaaa
    10561 aataacgtga aaaaggaaat ataaaataac atggagcgtg ggaggaataa agaaatttta
    10621 cacgaaaggt gcgcagggat gtaaagaaac ttaacacgaa acaaaagt
//
LOCUS       DAZKCC010000096        10645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000096 DAZKCC010000000
VERSION     DAZKCC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.098"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..661
                     /locus_tag="ACG8VH_002548"
     CDS             <1..661
                     /locus_tag="ACG8VH_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9111060.1"
                     /translation="LLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKEN
                     LLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVI
                     QTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIES
                     FHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKM
                     A"
     gene            706..1893
                     /locus_tag="ACG8VH_002549"
                     /pseudo
     CDS             706..1893
                     /locus_tag="ACG8VH_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2013..2213
                     /locus_tag="ACG8VH_002550"
     CDS             2013..2213
                     /locus_tag="ACG8VH_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9111061.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            2289..2486
                     /locus_tag="ACG8VH_002551"
     CDS             2289..2486
                     /locus_tag="ACG8VH_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111062.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(2783..4330)
                     /locus_tag="ACG8VH_002552"
     CDS             complement(2783..4330)
                     /locus_tag="ACG8VH_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9111063.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(4414..5217)
                     /locus_tag="ACG8VH_002553"
     CDS             complement(4414..5217)
                     /locus_tag="ACG8VH_002553"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9111064.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(5220..6002)
                     /gene="phnE"
                     /locus_tag="ACG8VH_002554"
     CDS             complement(5220..6002)
                     /gene="phnE"
                     /locus_tag="ACG8VH_002554"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9111065.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(5999..6778)
                     /gene="phnE"
                     /locus_tag="ACG8VH_002555"
     CDS             complement(5999..6778)
                     /gene="phnE"
                     /locus_tag="ACG8VH_002555"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9111066.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(6750..7541)
                     /gene="phnC"
                     /locus_tag="ACG8VH_002556"
     CDS             complement(6750..7541)
                     /gene="phnC"
                     /locus_tag="ACG8VH_002556"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9111067.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(7602..8531)
                     /locus_tag="ACG8VH_002557"
     CDS             complement(7602..8531)
                     /locus_tag="ACG8VH_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9111068.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            8726..9409
                     /locus_tag="ACG8VH_002558"
     CDS             8726..9409
                     /locus_tag="ACG8VH_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFP9111069.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            9570..10613
                     /locus_tag="ACG8VH_002559"
     CDS             9570..10613
                     /locus_tag="ACG8VH_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9111070.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         3307 a         2159 c         2092 g         3087 t
ORIGIN      
        1 tttattaaaa gaagggattt cagtatctct taaacatgtc caaaagctaa tgaaacaatt
       61 aaatttaagg tcgattgtag ttaaaaaata tagacctcaa aggtctaata aaccgatcat
      121 ttcaaaagag aatctcttaa atcaggactt ttctactgaa acgatctgtg agaaatgggc
      181 agctgacatt acgtacattc ctactaagaa aaatggttgg tgttacttat cttcaatcat
      241 ggatttacac acgaaaaaaa ttattagtta tacattttca aaacgaatga ctgtggattg
      301 tgtcattcaa acattgaata aagcaaaaat acattatcac attccagaag gaatgattct
      361 acacactgac ttgggcagcc aatacacagc aagagaagtg gaacaatggc ttaaaaccaa
      421 caaaataagg cattcttata gtagaaaagg aacaccttat gataatgctg gaatcgaatc
      481 tttccatgcc tcattgaaaa aagaagaagt ctacacgact agctactcag attttgaaga
      541 agcaaatcga gcgctattta gctacattga aggattttat aaccgaaatc gaatccatag
      601 ctcgattcac tatctaaccc cacaggaatt tgaagaatta gcaaaagaaa aaatggctta
      661 accgtctcta ctaaaatgtg tccaagatat tgactcaaat ccatgattta caaaggaact
      721 cttctgtaca agccagaaga gtgttcccat tgtctttgtg tcgtcccctc taggatcatt
      781 cgttggggga cgacaaccgt tcggttgtta ctgaatgatg cgtctgagta tcgcacttat
      841 ctggagttga agaaacaacg atttaaatgt aagtcttgcc aaagaacatt cgtagcggat
      901 acctctgttg ctgaaaaaca ctgtttcatt agtcaaaaag ttcgttggtc tgtcattgct
      961 cgcttaaaag agaatacttc catgacggaa atcgctcgtc agaaaaatat ttccacttct
     1021 tcggtctatc gtgtcatgaa gcgattctac cgcccattga atcctttcaa acaggctttg
     1081 ccaaaggtct tatgctttga tgaattcaaa tcggttcgaa gtgttgccag tgcaatgagt
     1141 tttatcatga tggacggaca aagccatgca ttattggata ttgtcgaaaa tcgacgcttg
     1201 ccttatttag aatgctattt ttctcggttt tcattagcga ctcgtgaagc cgttcaatgg
     1261 attgttattg atatgtatgc gccttatgtt tcattggtga aaaagctttt cccgaatgct
     1321 cagttgatca tcgaccgttt ccacatcgtc caacatattg gcagaacctt cttgaatcat
     1381 cgagtgaaag aaaccacggc acggcttaaa ggaacttccc actctcaacg agggttaggg
     1441 aaaaaattga aacgctattg gaaactccta caaaaagaag aagcaaagtt aaactacgaa
     1501 aaaagggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat tgtcgatcga
     1561 ctgttatctg cttgtcccaa cctgcgccaa ggctatcagt tataccaaga cattctttat
     1621 gcggtaaaga aaagagatca gtcattattt gaggactgtt taaacagaga agtcagcggt
     1681 cttcctgaaa cctatacaac cactttacga acgtttaaaa agtttctgcc acagatccaa
     1741 aatgcgcttc actacagcta ttcaaatgga ccattggaat gtttaaacaa ccacatcaaa
     1801 gtattaaaac gcaatgcgta cggctttcgc agcttttaca atttcaaact acggatcatg
     1861 attcgtcatg gcaaaacatt tttaaccaaa taaacagaac agaaagagag tttcttcttt
     1921 ctgttctgtc caaattattc taagatttct ctttaccaac atcatttgac aaagagcctg
     1981 tatttattac tgtcgaggac aatggcatcg gcatgattga agaggaacaa aaaaacattt
     2041 gggatcgcta ttataaagcc gatccgtcaa gtaaaaatac aaagataggc gagtcaggtc
     2101 ttggattatc aatcgtgaag gaactagtgc gcctgcacca cggttcaatt gaagtgacca
     2161 gccgaaaaaa tgaaggaacg accttcctga tacaatttcc aaaagaaaaa taaaattacc
     2221 agtaaaaaac cgtggagaga tagctgctat tgatttttgc tatgtctttt ctttgagagg
     2281 tatccggtat ggtggagtgt ctgaaaaata aaaaaatgga ggccaatttt gtgaaagctg
     2341 ttatttctgg aagtattgtt ttccaagaag aatatcgaga tatggaaaaa gatttcagta
     2401 aaaaaggcat tgaattaatt gattatcctc gctcatgtcc gatcctagac aaagagtatc
     2461 cagaaatctt gacgactttt ttttaaatat cgcagcagcg gatttatttt ttgtatttaa
     2521 tcaagatcgg aaaggggtat taggctatat tggtgccgca actttttcag aaatgaagta
     2581 ttgtctgatg caaaatctta ttcatggaaa aaggatcaaa atagtcttat ttaaggaacc
     2641 atcagaggaa aattttttgt tttgatgagg tagatcgctg gctaaagaat ggctggatgg
     2701 aaatcagaca gcctaacaat ttggcataac aaaggtgtgg gcaaaaaact gctcacacct
     2761 ttttgaattt tttatttata tactacaaaa tcacttgaaa attatgattc acttttgctt
     2821 cgatgcgtgg gtgaatattc aaatagtcgg caattaattc ggtcatgtcg atttggattt
     2881 ctcggacaat tttctccgca ccaaacatcg cataatttcc tccgccgatt gcccgatatt
     2941 gatttgtcac cacttccagc atttgttgcg gaagaacggg gtgaccttta aaacgaagtt
     3001 cggtaatccg ttgtccaaca ggctgtttca tatcaatagt gtactgaatc ccttcataca
     3061 tgtcataatt gtagtactgg ggcttaggct gaataaaggc tggattgaat acgatttggt
     3121 tattttctac agctaaataa tttgcggtct gctccaacgc cgctctcaaa tctgcaccat
     3181 ttatttgaat cactgccaga gtattcggat aaatatagtt ggtgatgacg tcccgcatcg
     3241 tgatcgtctc accaaagcct tttccttcat tattgaataa cgcagttcca gaaatctctg
     3301 agccgctggc aaataattgt acacgattaa taaactctac atagggatgt tcctcaagac
     3361 gagcctgcat cggatcatta atacgcatat ttccgttcac tttacctaag ggttgatcca
     3421 accatttctc tagtttttct tctaccggta cgatcgctgc aagaatttct ggatcaggct
     3481 ctgatttttc aactgagtgt aaagcaacct ggtgatctgt aacaaccact tgcccctctt
     3541 ttttctcgac ggtaagacga atttcaccga tgaactcgcc acgatagcct ggttgaacaa
     3601 cgggaactcc atgaaccgtt tcagcaattt tccggtgctg atgaccggtg aaaagggcgt
     3661 caatgccttc aacctgctgc aatagctcgt agccttcatt ctctccagtt agcgcttctg
     3721 tgtcctcacc ggttaacaag tctttttcaa atccgccgtg gtaactaacg attaccaagt
     3781 ctgcttgttt ccttaataga ggaacatatt cttttgctgt ttcgacgata ctcttaaacg
     3841 taattcctgc taaggtttct ggcttttccc agtgaggaat gtattgtgtc gttaatccca
     3901 agatagcaat ttttacaccc accttttcga taatcttata ggcgtttcca aagtatggtt
     3961 ggtcattttt gttcaccata ttggctccta atataggcgc ttgataattc ttaatcgctt
     4021 cctcgagata atccacacca tagttaaact catgatttcc caacacttga acatcatagc
     4081 tcatttggtt aatcaagtct gtaagctctt tggctgaacc atattcttta gcaagataat
     4141 aactcaaagg agacccttgg atgaaatcac cattttcgat cgtaattacc ggtccatctg
     4201 cttttgcccg taattccttc agtttcgacg atactttagc cgttcctaag gccagatcgg
     4261 ttctgccgga atagtctgtt ggtgaaacat agccatgcat atcacttgtc tctaaaaacg
     4321 taatgttcat tctgcgtgct cctttagctg actcaaaata gaaaaaccga agcgtattca
     4381 gctgttcggt ctcttttttt gatttataat gccttaatcc ttcatttgaa gtgtttttaa
     4441 aatgttgcct ggaataaagt tttctgttgt ctggcgaaca atccttcctt gatcgagatg
     4501 ataaaggtta tacccatgac gttcttcaaa gcgtacgaag ccatcgcctt catcttcacc
     4561 aacaaaggac atactcgata cagtaaaata ggggatccca ttaatttcat cagtaaatgt
     4621 gtgatgggta tgaccgttta ggatacagcg aatatcgtaa ggtgcgatta gcttcaagaa
     4681 acgttcggta ccaggccatt ttggtatact gctttggtga gacagtagat gatgatgggt
     4741 gaccaagaaa atcggttggt ctttttcttg ctctaaggct tttttcagcc attgaaattg
     4801 ctgatcgtcc actataccat ctgcgtatcc atagcttgaa ttatcaaagg aaatcactgt
     4861 aaactcagga tatttgacta tctggttgta cggggcagaa gaggcggctt catcgcacca
     4921 gcctacccaa aaatgttctt tgatatcgtg attgcctaga gtaacaagaa tcgtcgtttc
     4981 tcctagcgca ttttccagcc aagtcttcaa gtatcgataa tcgtctattt cgccatcttc
     5041 ggttaaatct ccgctgataa tcacaaggtc caagggtgtt tgctgcagga gatgatgaat
     5101 gcattgctcc agagggataa ggggattctg catcgtagcc agcattccct tatagccttc
     5161 ttcacaagcc tcatactctc tgcgaaagtg tatatctgat atatgcaata tattcattct
     5221 tatctcaatc tctttcttat aattccggtt atttgatcta aaataaacac ggttacgact
     5281 acgccaatca aaataatact cactttgctc caagaccgag tttgaatcgc gaatatcaat
     5341 ggagcaccga ttccgccagc gccaactaag cctaaggtag ccgcactacg aaccgctatt
     5401 tcaaaatgat ttaatgctaa agaacaataa atcggcatca aataggggat gcgaacaaag
     5461 aaaatcgttt gccaaaaatt tgctcctacc gcatgcattt cttcaacaag tcgctcatcc
     5521 atcgcttcca tttcttctgt aaaaagtttg ccaagcattc cgatctgatg gacgccaatc
     5581 gccataacac cagcaaaggg gccaggtccg acgactttca caaaaataat tgcaaaaact
     5641 agttcaggaa aagctcgtaa aacattacag ataagcttgc caatttttgt tacccatgga
     5701 tgtgcttgcc agaggttaac cgcactgatc aaggtaatgg gaatcgctaa taccgtagcg
     5761 atcattgtcc ctaaacaggc gattccaatg gttaataata gcaaactaat caaatcctcg
     5821 ccgctgccat catatagaaa ctgccaatca ggctgaccta accctctcaa aacgtccatt
     5881 cccatggaag gagagaaggt gcttaatcca gagtaatcca gagtagcggc cgacaaaata
     5941 acgagaatca ctaaagcaac taatgataaa agggttcttc tgttcatttc acgaagcatc
     6001 ataacaggga cctcctgaca cgttcactta atttatcaac gatcgcgaca gttactagga
     6061 tcgttagaat aatgattgaa actcgttcat aattcaaaag cgcaagtgag gaatttagaa
     6121 taacgccaat accaccagct ccgacatatc caaggacagt agaagcacga acattcacct
     6181 caaacgcgta aaaagcatag ctcgcaaact gactcaaaac ttgtggagcg atcgaccaaa
     6241 tagcgatttg cattttattt gctccaacag ccgcagcggc ttcaatcggg ccaaagtcaa
     6301 tggtttcaat tgcttgaaac attaattgtg acaccatacc aaaagtaaag acagcgatag
     6361 tcaacacgcc agtcgcttca ccaattccaa acacagcaac aaaaagcgcg gctaaaagca
     6421 gggtagggat ggtccgtact aaacctaaaa gaaaacgaaa gatggtagaa agaagacggt
     6481 tttctgtcac tacagtggtc gctaaaaaac taacagggat cgctaaaatt aagccaatga
     6541 ccgttccaag caaagacatt tgaagtgttt tcaccatagg ctcgactaat ttaggtaaat
     6601 agctaaagtc tggatgaatg aagcgtgata aaaagacagt catttgattg gagttttgaa
     6661 aaacctgaga aaaatcagct gacgtgatcc aggcacttcc ttgaaaacaa acaattaaga
     6721 acagaagaat aagcaggtgt ttataccatt taaagtgaac ggtttctttc aagctcatcg
     6781 atttcgcctg ccttttcttc aaagattgca ttgccataaa tttgctctaa gcgttcatcg
     6841 gttaagtcgt ctggacgacc atcaaagaca acctgagcag cttttaacgc gagcacacgc
     6901 gtagaaaact cacgagccag agggactgaa tgcagattga caatgactgt tttatccatc
     6961 gtttgattga tattttttag gtccatcata accttttgag tcgtgattgg atctaatgat
     7021 gcaacgggtt catccgctaa aataatgggg gcttgctgca cgagtgctcg cgcaatggat
     7081 actcgctgtt gttgcccacc actgagctca tcactgcgtg tctgcaattt atcagacaaa
     7141 ccaacttttt ccagtgcttc ctttgtctgc tggtagtctt ccttcgtaaa aagacctaaa
     7201 atacttttcc aagtggagta atagcccaaa cgtccagata gcacattttt ctgaaccgta
     7261 ctacgcttta ccagattgaa gctttgcgag attagaccaa tctggcggcg aatctgtcgc
     7321 agctcttttt tattcgcttt tgtaatagag gttccattaa tgcgaatatc tcctttggtg
     7381 gtttctgcca agcgattgat actgcgcagc agagtgctct ttccagcacc actcaaccct
     7441 atgatggaaa caaattctcc atcttcaatt gttaagtcaa tatccttcaa gccaacaacg
     7501 ccattgggat agaccttcga aacattgtca aaaataatca ttatccttct ccattttttt
     7561 aagtgtaagc aaaaaaaagc ttgctgacac ttactgatgt tttattctgc tgcttttttc
     7621 gtgtagtctc tgatcgtgtc ataggcagaa tcagcagctt cttcatagcc ctgatgcccc
     7681 cacaaagcca ttgcttcttt gccatcttta tcatccttca tatttaagaa ggtctcttta
     7741 attttcgctt ttaaatcttt tgacatgtca ggctgtacag cgattgcgtc attcggaata
     7801 tcgccttttg tcaggtacag gacttttaaa tccttaaata aatcattatc tgggaaattc
     7861 gaagcaaaaa catttcgcgc gccttcaaaa acaaacgccg catccatttg cttgttcaac
     7921 actgcggtaa tctcactcgg aatatcattc acagtggtca tctttgtatc tgcagtagga
     7981 tcaatcccag catctttcaa ttcagcgaca ggatagatgt agccgctagc agagttagga
     8041 cttaaggttg cgatattttt tccttttaaa tcagttaact tattcaatcc gctatcggca
     8101 cgaaccaata cttctccttt aaaggtgttc gtaagtttgt cctttaatgg caagccagtt
     8161 tcttgatcat aatcgccgag ctgagaagtc aggattgcat ctgcagctcc agcatccttt
     8221 gcttgaacat aggctgcagg cggcataatt ccgacatcga cttgaccaga agacatcgct
     8281 tcgacgatcg ttgaataatc agtagctaac gtcacttcta cctcacgatc taactttttc
     8341 gataaatact tagcaaatgg ttttgctttt gcctccatac taccatcatt gtttgttgga
     8401 acgaattgaa caactaattt ttctttgctc tctttttctg agctgctact tgtattccca
     8461 gaaccgcagg ccgccaaagt ggtcaataac atgactccga tgaacccttt tgctaactgt
     8521 ttgaactgca tttttctcct cctgttggat ataatttcta atacgtctaa atcatagcag
     8581 ggaatcaaaa tcaatgaagt gacgcacgtc ctattttaca caacttttac atacaattta
     8641 caaacaatta taaataatta ttcgtattga gccgaaaaac aatctaagct ataataaaga
     8701 aaaacttctt agaggtgacg atttcatgaa aaaaatccat gatacgaagc aattaactgc
     8761 ttacgttgag aagcacaacc tgcaggatta tatggattca gatatattgg caatggcttc
     8821 tttacttttt tttgaaaagg aagagcacct cattcaaacg ggaaaaacct cagattattt
     8881 gtatttttta gtggatggga cggtaatggt gtactcgtat tcttctgata cacaaaatat
     8941 ttgcatcgat tacgcaaagc ccgctacacc actcggcgaa gcctcttcat tgtgggaatt
     9001 atcgccgaaa agcagcgtta aagcagtgac taagtgcatc tgtatcagca ttccgctaaa
     9061 tcaatatcga acacagcttc aaaaagatgt tcgctttttg cagaatattt gccagttact
     9121 aagttatcgt ttaaattccg ggataaattt ggcgaactca ctgacagaac ctgttgaaac
     9181 tcgtttagca aagtttattt tgacccatca cgaaaacgaa cgtttctcat ttcggttaac
     9241 cacctgcgcc tcaatcttaa atgtcagtta tcgacatctt ttacgaacaa tcacgaattt
     9301 tcgtgaggag aatattatcg aaaagaaaaa gaattattac ttgattcatg atatcgaagc
     9361 tttagaggac ttagctgaga atataagtag tccagtaaaa gcagaataac agctataaaa
     9421 aaacagcagt cagaagtgat tgctggtttt ttgatagtgg agaaattggg aaacggaaaa
     9481 tcttcgttga agagtagtgt tctatatatt ggtatgctat acttttaaaa gttcaatgtg
     9541 tcaagacaca taaaaagggg agaaaaattt tgaagaaaat acttgtttca ttgttgtttt
     9601 tggtgtcatt agtaacgtta gtcggctgta gtcagtctca gggggagaag caaagtgaaa
     9661 aagagaaaag taaaatcgct ttggttcttg gatttggcgg ggttgatgac cgctccttca
     9721 atcaagctgg ctgggaagga ttagaggctt ggggaaaaga gaaaaagctg acagcaaaaa
     9781 aagattttac gtatatagaa acgaacaatc cagaggataa cagtaccaat atcgatcagg
     9841 caatcagaaa cggctttacc accattatta gcttgagcta cttgcaagca gaggaactgg
     9901 aaaatgctgc taaagataat ccaaagttga aatttgcctt ggtagatggt gagattactg
     9961 ggaaaaagaa tatctcatca gcaaccttta aagatcaaga aagtgcgtat ttagcaggac
    10021 tagctgccgc atatacgaca aaaagtgatc aagtaggctt tattggtgga atggaagggc
    10081 ccgtgatcca acgtttccaa agcggatttg aagctggcgt taaggcaggg gccacagcac
    10141 tgaacaagga aattactgtt aacgtacaat atgctgcatc ctatgattcg ctagataaag
    10201 gaaaagctat tgctgcttct atgtacaaag agggagtcga tattctttat caggcggcag
    10261 gaagtactgg agctggggtg ttccaagaag caaaagcccg caatgaaaac gactatagca
    10321 gaaaagtatg ggtgattggt gtagatcgcg atcaagcgga tgaaggggat tataaaatgg
    10381 agaacggcga gacagggaat ttcacgttga cttctacaaa gaaaaatgtc ggtgcagaag
    10441 tcaaagaatt tgcagaaact tctcataacg ataaatttcc tggcggcaag catttgacct
    10501 ttggtttgaa agaagaaggg gtagatctga caaaaggaca attatcagag gaagcctatc
    10561 aggcagtttc agacgcaaaa gagaaaatca tcagtggtga tttcagactg tagacaaagt
    10621 ccattttatg gatgatgtct acagt
//
LOCUS       DAZKCC010000097        10517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000097 DAZKCC010000000
VERSION     DAZKCC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.099"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG8VH_002560"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG8VH_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG8VH_002561"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG8VH_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG8VH_002562"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG8VH_002562"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9111071.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8VH_002563"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8VH_002563"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP9111072.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG8VH_002564"
     CDS             5704..6489
                     /locus_tag="ACG8VH_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9111073.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG8VH_002565"
     CDS             6502..7353
                     /locus_tag="ACG8VH_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9111074.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG8VH_002566"
     CDS             complement(7477..7929)
                     /locus_tag="ACG8VH_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111075.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG8VH_002567"
     CDS             complement(8394..8762)
                     /locus_tag="ACG8VH_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP9111076.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG8VH_002568"
     CDS             8912..9394
                     /locus_tag="ACG8VH_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP9111077.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG8VH_002569"
     CDS             9431..10441
                     /locus_tag="ACG8VH_002569"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP9111078.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAZKCC010000098        10252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000098 DAZKCC010000000
VERSION     DAZKCC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.100"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            364..1599
                     /locus_tag="ACG8VH_002570"
     CDS             364..1599
                     /locus_tag="ACG8VH_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9111079.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2598..2858)
                     /locus_tag="ACG8VH_002571"
     CDS             complement(2598..2858)
                     /locus_tag="ACG8VH_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9111080.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2885..4186)
                     /locus_tag="ACG8VH_002572"
     CDS             complement(2885..4186)
                     /locus_tag="ACG8VH_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9111081.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4478..4936)
                     /locus_tag="ACG8VH_002573"
     CDS             complement(4478..4936)
                     /locus_tag="ACG8VH_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9111082.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4949..6280)
                     /locus_tag="ACG8VH_002574"
     CDS             complement(4949..6280)
                     /locus_tag="ACG8VH_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFP9111083.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6291..7733)
                     /locus_tag="ACG8VH_002575"
     CDS             complement(6291..7733)
                     /locus_tag="ACG8VH_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9111084.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7720..8484)
                     /locus_tag="ACG8VH_002576"
     CDS             complement(7720..8484)
                     /locus_tag="ACG8VH_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP9111085.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8588..9463)
                     /locus_tag="ACG8VH_002577"
     CDS             complement(8588..9463)
                     /locus_tag="ACG8VH_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9111086.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9718..9802)
                     /locus_tag="ACG8VH_002578"
     tRNA            complement(9718..9802)
                     /locus_tag="ACG8VH_002578"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9766..9768),aa:Leu,seq:caa)
     gene            complement(9820..9891)
                     /locus_tag="ACG8VH_002579"
     tRNA            complement(9820..9891)
                     /locus_tag="ACG8VH_002579"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9857..9859),aa:Gln,seq:ttg)
     gene            complement(9901..9973)
                     /locus_tag="ACG8VH_002580"
     tRNA            complement(9901..9973)
                     /locus_tag="ACG8VH_002580"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9938..9940),aa:His,seq:gtg)
     gene            complement(10035..10119)
                     /locus_tag="ACG8VH_002581"
     tRNA            complement(10035..10119)
                     /locus_tag="ACG8VH_002581"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10083..10085),aa:Leu,seq:caa)
     gene            complement(10140..10210)
                     /locus_tag="ACG8VH_002582"
     tRNA            complement(10140..10210)
                     /locus_tag="ACG8VH_002582"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10176..10178),aa:Cys,seq:gca)
BASE COUNT         3311 a         2145 c         1584 g         3212 t
ORIGIN      
        1 tgtcatggcg agaaagtcta agaatatttt taaacataac tatttctttc ttacgtgtgt
       61 ataatgacga cagcacacct atcaattaat ataattattt tattttttta tgttctcatg
      121 tttattttta atgacacaac ttctcgtatt tatctggcac aacatataaa tcgatcaaaa
      181 aattgttgcg tgtttttttg aaaattggag tttcaacttg attacaaaat ttaaatttag
      241 aagttgtgaa aacagcgaga gacagtttaa tattcaaaat atacataggc tactcgaacg
      301 ctgtgttttt tagtatacta gtaagaaacg aagggataga ctcattccaa taaggaggat
      361 ttcatgaaga caattctttt tgctcctgca gtattcaatt tagctgagac aacacgaatg
      421 attgaggttg ccaaagctat gaaagaccgt tattactgtg tattctttgg cttttctaaa
      481 acttttgctc ctttgatcac agaaagtggc tttgagtatc atttactagc gccgatcctt
      541 acgaaaaaac aagaagaaca aatcataaaa tttgaccagt tgcgtacact taaaaaccct
      601 tttaccaaag aaatggttgc ctctcgtatc actagtgaac agagtctgat tgctaagttt
      661 gagcctcgac tgattgtgac aggttcgaat gtgacaattt ttttatcggc ccgcaataaa
      721 aagattcctt tagtatatgt taaaccctat gctttgagtg aacctttttt tcaatcagag
      781 catgcgctga tgccccacga gttacgtttc ttcggccctt taagcaatcc cctttggcac
      841 tggacaaaaa agcgtatagt aaatacccga tggatcccca atgccttcaa acacgctgca
      901 caaaaagaaa tgcttccttt accaaaaaca agtttacaaa tgatggatgc tgatctaaat
      961 ctcatcacaa cgcctaaact atttttggaa gaagaacagc tgccggaaaa ttatcgaatc
     1021 gttggtccaa tttttgctaa gctcagtact tccttgcctg aagaagtgct aacttttata
     1081 cagcgaaagc aacaagaaag aaaacgtatt atctatttcg caatgggtag ttcaggcaat
     1141 ccgcgtatgt tacgcaaaat tttgttcttc ttacaaaaac aaccggatct agcggttatt
     1201 gcacctattg actatctgct tgatgacatt caatctgact cagaagatct tttaataaca
     1261 gaatatctgt ctagtcatct tttgcatgaa ttcattgatt tttcttttat ccacggcggg
     1321 gaagggaccg tccaaactgc ctgtgaatcc ggcaaaccgt ttatcggtgt ggggatgcaa
     1381 tttgagcaaa gctgtaatat tgactattgt gtaaaatacg gaaacgcaat cgccctcaca
     1441 aaaccagtga ctgaaagaaa actgcagatt gctctgcgcg aagtttcttc acccaagatt
     1501 cgtcagcagg cacgcacgct gcaagccgtt tttccaataa atggtccaca acaagcaatt
     1561 caagaaatcg aaaccttcct gagcaaccac ccaatatagt ttcaagtaga aaaagctttt
     1621 ttaatagaga ctcatcagta atttcgctat tcgagaccca gacagtgaaa atacaaaaaa
     1681 agtaaatagt ggattgaaaa gttagagtta cttaaattac ggtgtctaaa aaacacaaaa
     1741 tcatccttaa aataaaagat ctgacgctat ccaaatgagc ataggtagcg tctgccattt
     1801 attcttagtc aaattctttg acacctttct ctggttggat catctcaacg tttcttcaaa
     1861 agaaagacca taataattag gcaaatacat tttcttaaaa tgtgtcatat agatctagaa
     1921 gcatcgcttt gtagagattt gtgccgctag tcccctcaac aattactaaa tcaaatgtag
     1981 gatttaccac tttatctttg ataatcaacc tccctcttct gttgagacaa tgatctcgca
     2041 atccgatgca tactcctata taaactgcca gcaatcccca catattgagt agatcatgtc
     2101 agaatgttct atgtcgtcag caacgataca ctaaaagtca cgttcaccat gactgattgc
     2161 tattcccagc acaattattt ctatacacgt ttcatgattt gaataaacac tcacatcata
     2221 catattacca ttcatacacc atactgcagc ccctactata tggttgtaag ctcccaaatc
     2281 tatatttgac ttgatcactt tggtagtaga tctactaatt ctaaatccat tgctgataaa
     2341 atttccattt tttcctcctt ctttctgcca aaacagttaa aacaataata atctttatgt
     2401 aaaaaagggc tgtgacaaaa gccttgtcac agtcccatat tccgaataaa cggtggtcaa
     2461 aagcttgcat ctgcggtaat aagttcaacc aaacgaaaaa taagccaaca tttcacaaaa
     2521 atttaagaag caattttcgt aaatttcttc ttatttcttg aagctaacca cttctgttac
     2581 aaccactttt agaacattta aagcttcgat gtgacaaacc atctatgctg ctcgatcata
     2641 aaattgatag caaaaattcc acaaagtaca tatgtccccc atgaaaacaa aacattcata
     2701 tgcggtacaa tgatctttgg aagaatcacg atcaaaaatg atgccagtgc ccaaatccat
     2761 cgattgattt gaatatgata tgtagctagc atattcaata taaggtgact agccgttaat
     2821 aaaaagataa aaacaaaaaa taaaatcagc caatacattg tttcccacct tctaaaaacc
     2881 aaacttagac agtctggact tcgttttctc cacgactttg ttcatctgac agcatctttt
     2941 tatcataagc ttttacaaat ggataataga taataaatgc tatcaatgtg ttgatcatga
     3001 tcaagataac aacacgccag tcatttcctg ctgaaagata accgccgatt ggagctggta
     3061 atgtccatgt tggcaaaaca gtaaatctat tgaccatttc taatttgact gcaaaataac
     3121 tgataatagt caaaactaaa ggaactaagt tgaacgggat gaacatgtaa ggattcaaca
     3181 tcataggagc tccaaaaatt atgggctcat tgatattgaa gataccagga ataatagaaa
     3241 aacgaccaac ctctttcaag aattttgatt tacagaagaa tataaacaaa acagatacca
     3301 cgatcgttgc tcctgcacca ccaattgtta ccgtccattg ataaaactgt tcaggagcga
     3361 tatagggtaa ttgggtggca gctgtaccat ctgccaatgc ctttgcattc tcatccaaca
     3421 tgatcagcca cattggacga acaacagccc ctacaatact catgccatgg ataccaaatg
     3481 accacaacaa atggatcaaa aacattggta gtaaaacacc aagcaaatta ttccctaaaa
     3541 tattggttaa aggctcgaat aacgataata aggcagcgtt tacatcaaag cccgcgacgc
     3601 ttcgaatcag ccaagtaatt gttaaaatga ctgcacctgg aatcaatgcc tcaaaactac
     3661 gggcaacagc aggtggcact tgttctggca ttttgatagt aatatttcga tttttaaaga
     3721 aacgatatac ttccactgca aaaatcatag ataagatccc cgcaaacatc ccagatgctc
     3781 ccaaagaact cataggtaag acccagccaa gaggtgcctc ttcaggagtt aaaacgttta
     3841 caggaacttg taacataaag aacgttccta aagataatat accacctgtc acactatcta
     3901 atccatatga tttagctaga tccgaaccca taacaaaagt tgcataaata gccatcaatc
     3961 ccatcgtcat cctaaagggt aaaacaatcg ttgccgaata aggggcaata aattcattcc
     4021 atccaggaaa cggtaaattg ataaagacca taaaaaatgt cccaatcaaa atcaatgaca
     4081 atgttgaaac aaccccatta cgaattgcta ataaatgacg ctgtgtacca atttttgcca
     4141 ttggtgggac aagtttctct tcgacccatt tcatcagctt ttccactaaa accactcctt
     4201 ttcaataaaa tatgaaccat tcattgcatg acaacgcaaa gaatgttcat aactaattat
     4261 ataattaaga aattttattt caatatattc tcttaaataa tcaaatttat ttttaagaat
     4321 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
     4381 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
     4441 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
     4501 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
     4561 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
     4621 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
     4681 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
     4741 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
     4801 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
     4861 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
     4921 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
     4981 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
     5041 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
     5101 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
     5161 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
     5221 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
     5281 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
     5341 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
     5401 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     5461 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     5521 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     5581 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     5641 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     5701 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     5761 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     5821 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     5881 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     5941 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     6001 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
     6061 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     6121 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     6181 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     6241 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     6301 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     6361 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     6421 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     6481 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     6541 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     6601 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     6661 aaagcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     6721 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     6781 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     6841 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     6901 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     6961 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     7021 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     7081 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     7141 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     7201 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     7261 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     7321 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     7381 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     7441 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     7501 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     7561 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     7621 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     7681 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     7741 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     7801 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     7861 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     7921 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     7981 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     8041 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     8101 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     8161 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     8221 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     8281 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     8341 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     8401 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     8461 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     8521 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     8581 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     8641 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     8701 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     8761 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     8821 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     8881 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     8941 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     9001 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     9061 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     9121 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     9181 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     9241 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     9301 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     9361 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     9421 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     9481 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     9541 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     9601 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     9661 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
     9721 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
     9781 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
     9841 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaaa
     9901 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
     9961 tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa
    10021 gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga
    10081 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa
    10141 ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg
    10201 ctataccgcc attatactat tatcaatcta taaaataaag aataaattat ag
//
LOCUS       DAZKCC010000099         9811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000099 DAZKCC010000000
VERSION     DAZKCC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.101"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG8VH_002583"
     CDS             complement(34..2154)
                     /locus_tag="ACG8VH_002583"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9111087.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG8VH_002584"
     CDS             2384..3562
                     /locus_tag="ACG8VH_002584"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP9111088.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8VH_002585"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8VH_002585"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9111089.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8VH_002586"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8VH_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP9111090.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG8VH_002587"
     CDS             complement(4915..6168)
                     /locus_tag="ACG8VH_002587"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9111091.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8VH_002588"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8VH_002588"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9111092.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG8VH_002589"
     CDS             7746..9092
                     /locus_tag="ACG8VH_002589"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9111093.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG8VH_002590"
     CDS             complement(9400..9786)
                     /locus_tag="ACG8VH_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9111094.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAZKCC010000100         9595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000100 DAZKCC010000000
VERSION     DAZKCC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.102"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..643)
                     /locus_tag="ACG8VH_002591"
     CDS             complement(<1..643)
                     /locus_tag="ACG8VH_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP9111095.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTK"
     gene            complement(645..1409)
                     /locus_tag="ACG8VH_002592"
     CDS             complement(645..1409)
                     /locus_tag="ACG8VH_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP9111096.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1417..2094)
                     /locus_tag="ACG8VH_002593"
     CDS             complement(1417..2094)
                     /locus_tag="ACG8VH_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP9111097.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2131..3516)
                     /locus_tag="ACG8VH_002594"
     CDS             complement(2131..3516)
                     /locus_tag="ACG8VH_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP9111098.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3726..3810)
                     /gene="ffs"
                     /locus_tag="ACG8VH_002595"
     ncRNA           complement(3726..3810)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8VH_002595"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3833..4348)
                     /gene="tadA"
                     /locus_tag="ACG8VH_002596"
     CDS             complement(3833..4348)
                     /gene="tadA"
                     /locus_tag="ACG8VH_002596"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9111099.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4455..5312
                     /locus_tag="ACG8VH_002597"
     CDS             4455..5312
                     /locus_tag="ACG8VH_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111100.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5393..5728)
                     /locus_tag="ACG8VH_002598"
     CDS             complement(5393..5728)
                     /locus_tag="ACG8VH_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111101.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5795..7165)
                     /locus_tag="ACG8VH_002599"
     CDS             complement(5795..7165)
                     /locus_tag="ACG8VH_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP9111102.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7243..7779)
                     /locus_tag="ACG8VH_002600"
     CDS             complement(7243..7779)
                     /locus_tag="ACG8VH_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9111103.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8088..9569
                     /locus_tag="ACG8VH_002601"
     CDS             8088..9569
                     /locus_tag="ACG8VH_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111104.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3216 t
ORIGIN      
        1 tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta
       61 ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga
      121 ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa
      181 ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca
      241 tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga
      301 taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa
      361 ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga
      421 ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg
      481 tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata
      541 gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga
      601 acaatccagc atattccatc cactgccatc tcgaattttt catattagaa gctccattca
      661 tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt
      721 gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct
      781 gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg
      841 gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct
      901 gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata
      961 ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta
     1021 actacttttt cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct
     1081 tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca
     1141 tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg
     1201 tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt
     1261 tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat
     1321 tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct
     1381 aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt
     1441 aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga
     1501 ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac
     1561 tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg
     1621 agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt
     1681 gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg
     1741 attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat
     1801 ataatcatat cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc
     1861 ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc
     1921 taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc
     1981 caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt
     2041 agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc
     2101 accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta
     2161 atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc
     2221 aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt
     2281 attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat
     2341 tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata
     2401 gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc
     2461 acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg
     2521 attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc
     2581 cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa
     2641 gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg
     2701 cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc
     2761 ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg
     2821 gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat cattgattac
     2881 gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat
     2941 ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc
     3001 cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc
     3061 ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt
     3121 cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt
     3181 atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt
     3241 caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc
     3301 aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca
     3361 acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt
     3421 ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg
     3481 cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct
     3541 ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc
     3601 tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag
     3661 gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg
     3721 tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac
     3781 acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt
     3841 cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt
     3901 cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt
     3961 taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat
     4021 acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac
     4081 aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac
     4141 aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa
     4201 ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt
     4261 catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt
     4321 ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt
     4381 tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac
     4441 aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa
     4501 aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct
     4561 ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg
     4621 taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg
     4681 ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa
     4741 aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt
     4801 atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg
     4861 aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg
     4921 ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa
     4981 tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg
     5041 tggctttaca ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca
     5101 tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag
     5161 gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa
     5221 tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt
     5281 gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa
     5341 ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca
     5401 ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat
     5461 caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag
     5521 aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa
     5581 atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca
     5641 ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc
     5701 cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca
     5761 tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga
     5821 aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata
     5881 ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt
     5941 gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg
     6001 ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac
     6061 gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt
     6121 tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga
     6181 gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat
     6241 gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt
     6301 tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc
     6361 ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa
     6421 gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt
     6481 gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac
     6541 agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc
     6601 cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga
     6661 caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat
     6721 tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc
     6781 ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa
     6841 ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc
     6901 tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct
     6961 cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc
     7021 ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag
     7081 aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg
     7141 gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt
     7201 acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt
     7261 taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat
     7321 ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac
     7381 caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg
     7441 ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc
     7501 atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca
     7561 ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc
     7621 gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa
     7681 ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag
     7741 caaatattct ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca
     7801 tctacccata aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca
     7861 tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca
     7921 aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta
     7981 tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac
     8041 agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag
     8101 caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac
     8161 aagaattatc caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa
     8221 taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag
     8281 gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa
     8341 aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc
     8401 cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc
     8461 tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat
     8521 ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact
     8581 tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg
     8641 cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata
     8701 tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa
     8761 cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat
     8821 actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact
     8881 ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga
     8941 ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga
     9001 cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt
     9061 attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt
     9121 ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga
     9181 aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc
     9241 aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca
     9301 tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc
     9361 agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca
     9421 ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc
     9481 acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag
     9541 gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttatt
//
LOCUS       DAZKCC010000101         9555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000101 DAZKCC010000000
VERSION     DAZKCC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.103"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8VH_002602"
     CDS             168..362
                     /locus_tag="ACG8VH_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111105.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8VH_002603"
     CDS             631..930
                     /locus_tag="ACG8VH_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111106.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8VH_002604"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8VH_002604"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9111107.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8VH_002605"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8VH_002605"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9111108.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8VH_002606"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8VH_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9111109.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8VH_002607"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8VH_002607"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9111110.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8VH_002608"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8VH_002608"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9111111.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8VH_002609"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8VH_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9111112.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8VH_002610"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8VH_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9111113.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8VH_002611"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8VH_002611"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9111114.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8VH_002612"
     CDS             7521..8291
                     /locus_tag="ACG8VH_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9111115.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8VH_002613"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8VH_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111116.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2979 a         1949 c         1492 g         3135 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatc
//
LOCUS       DAZKCC010000102         9159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000102 DAZKCC010000000
VERSION     DAZKCC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.104"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..460)
                     /locus_tag="ACG8VH_002614"
     CDS             complement(<1..460)
                     /locus_tag="ACG8VH_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111117.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGIL"
     gene            complement(757..3138)
                     /locus_tag="ACG8VH_002615"
     CDS             complement(757..3138)
                     /locus_tag="ACG8VH_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9111118.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3128..3775)
                     /gene="recU"
                     /locus_tag="ACG8VH_002616"
     CDS             complement(3128..3775)
                     /gene="recU"
                     /locus_tag="ACG8VH_002616"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP9111119.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3839..4378
                     /locus_tag="ACG8VH_002617"
     CDS             3839..4378
                     /locus_tag="ACG8VH_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP9111120.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4468..4887
                     /gene="gpsB"
                     /locus_tag="ACG8VH_002618"
     CDS             4468..4887
                     /gene="gpsB"
                     /locus_tag="ACG8VH_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP9111121.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4909..5276
                     /gene="rnpB"
                     /locus_tag="ACG8VH_002619"
     ncRNA           4909..5276
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8VH_002619"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5485..6651
                     /locus_tag="ACG8VH_002620"
     CDS             5485..6651
                     /locus_tag="ACG8VH_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP9111122.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6697..8193
                     /locus_tag="ACG8VH_002621"
     CDS             6697..8193
                     /locus_tag="ACG8VH_002621"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP9111123.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8274..8957
                     /locus_tag="ACG8VH_002622"
     CDS             8274..8957
                     /locus_tag="ACG8VH_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9111124.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8966..>9159
                     /locus_tag="ACG8VH_002623"
     CDS             8966..>9159
                     /locus_tag="ACG8VH_002623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111125.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2936 a         1630 c         1910 g         2683 t
ORIGIN      
        1 ccaatatacc tataaaataa gtccagtatt ccaaaggatt atataaataa gtgacgtatc
       61 tatctaaagg tgtaataaaa atgataagtg ataataaaaa aataccattt ataactaaac
      121 ataaactaat ggatttgggc ggtttcaggc tcatcagaaa aatatagatt ctggtgaaca
      181 acaaccataa aaaagtgaga atcattgcat gagagcttct ttccataaat tttcctttaa
      241 gagtagtgct taaaagtaca gaaaaaataa taaatgcaag gataggagca attaaagtaa
      301 atttatttaa taataaacaa aatttttcag cttttttatt cttggaataa cgaggtattg
      361 ttttcgataa aaaaagatgg tgcagacaaa ttatgataat taagcatact atcagataac
      421 tcaaaatgaa gccttctttc aaaaaattat tcaaaaccat atattgtatt ttttatttga
      481 taataacaat gggattatta agaagtggca ctaaataaaa gactagtgtg tatttagccg
      541 atttaattat aaacaagtaa atggataaag tatatgtaat gataaaagaa aaatggattt
      601 tctaattttt aagctaaatg ttagatcaca catgtatatg ctggatagtc ttataaaaaa
      661 tcatgtaata cagcttggta catgattttt atttgtagct gttttttaca tagacacaaa
      721 aaagaaacca cgcttgtaga cgtggtttct tttttactat ctgtttccag aagctgaggt
      781 gctgctggat ggtgtatggt catttgcagc atccgctccg ctagttgctg gctcactgga
      841 agaagcagga ggagcagatg aagacggtgt cgagctttct tcggatgaag aagaaggaac
      901 cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg aggactcagc
      961 agttgattcg ctttgactag tagagctaga gctttgagtc gttgtctctg ttgttgagct
     1021 tccaggagtc tggacatagc tggaagatgg aatagttgtt gatggtgtaa ttgcattcga
     1081 tctagctgta tattgatctt tgtagtacag ttcaccgccc acacgaatta atccgcttgg
     1141 catctcccag tctgtatttg tcacgttagc tgaaacatac tgcataagct cgcgataaac
     1201 atcagaggct acgtgtgacg attctgaagt gacaggcgtc atttttttat tgtaacctgt
     1261 ccacacggaa atcgaataat ttggcgtata gcccgcaaat aggatatctg gatacacccc
     1321 agaagaaata ccaagttttg catactcgtc atctgtgtaa ttggatgttc cggtcttacc
     1381 ggcttgatat aacccagcga tttgcgcatt cgtaccagtt ccttcagtga tcgtatcttt
     1441 taaaatgtct gtgatcatat aagctgtttc tggagacatg gctgtttttc catctggctc
     1501 ataagtttct tctgttccgt cttggaagac gattttatta acatattgag gtttgtaata
     1561 agtccctcca ttggcgaaag ccgcataggc tgctgccatt tttaaggaag aagctccgta
     1621 ttttgtccca tcttgttctt ccgtattact agagatcgca ttcgattgat ggatcgtact
     1681 gtattcgatc ccaagatttt ttaagaaaga agccacttta tctgaaccga cttcattgaa
     1741 taatttgact gctggaacat tacgggataa gtaaagtgct tggcgtaaag taatggtgcc
     1801 catatattga ttatcccagt ttcctacagg agtcgaagtc ccttcatagt tatacggtgc
     1861 gtcagtaata gtttttccag tagaatattt caaatattca aaagctggtc cgtaatctgt
     1921 gacaggtttc atcgtagaac caaaatcacg ggaagtattc actgctaagt tgttgcctaa
     1981 agttacgtct tctgcgatgt gacgtccgcc aatctgggct tttactttcc cggtattggt
     2041 gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg taagagacat actgatccgt
     2101 gttgacgata tcatatagtt tcttttgggc gtccagatcc aaattcgtat aaatctctaa
     2161 accgtctgtg tagacattct tatctgtttt ttcttgtact tcattgatga cttctttgac
     2221 ataattatcg acgatcttag tattgtcatc ggattgagtt agttcttgca gaccatccgt
     2281 taccggtaca ttaacagctt gatcatattc tgtttgagag attttttcgt tttgaagcat
     2341 cgtatacaaa acagtatctc gtcttttctt cgcctgatct ggataaacat aaggatcata
     2401 ggcggaaggg gcttgaggca tcccagcaag caatgctgtt tgcggtaaag aaagttctga
     2461 taatttctta ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc cgtttgacat
     2521 atacacttta tttacgtagt aggtcaagat ctcttgtttc gatttttttt gttccaatct
     2581 tacagccatc cacgcttctt gggccttgcg tttcagagtc tgatcctcgg cactggtgga
     2641 gaagaaggaa agtttgatca attgctgtgt caaagtactt ccgccttgca gtccgccaga
     2701 ggtaaagtta gagagggcag aaccgatgat ccgaataggg tcaaccccga tgtgtttata
     2761 aaaacggcga tcttctaccg acacgatcgc atcttctaat gttttcggta attcattcgc
     2821 agaaattttt tcacgctttt ctgcgcctaa atcttcgaat agttcgccat cttgtgtata
     2881 tagtttggaa gatacggtag cgtctaattt tttatccgtc aattcgggcg catcttttgc
     2941 gtaataccag aaaagtccta cacctgctaa aaagaggata cagaaaaatg agagtattcc
     3001 taaaaggatc ttgataaata atcctttttt atgtgttcca gaggactttc cagaaccttt
     3061 gccagaattc ttcttcacac tctttttagg agtaggctgt ttctgtcgtc tagagcttct
     3121 agtttgttca tttgccattc ttgctttctc cttcaataga taatgttgat aaatagaagt
     3181 caaccgcgtc caaataagga atccgcggag aaataccagt tttgatctct attcctagtt
     3241 cttcgatcaa agataaaggt agtgatttgc gtcctttttc cttttgggcg tcccagtaag
     3301 caatcaaatg aacactattc aaataaaaac agcgcttcaa agacgaaaac cataacaaga
     3361 caaagcagac cccattttgt tcgaggcatg ctttcatatg ttcgatttga tgggcatgga
     3421 agtttttaaa gggaaaagat gttttgttct tggtttcttt tgcttcaaag tccaagtaaa
     3481 agcctcgata gacgccatta tagtctgtag ttgaggcttg ggtaaagtag gcttccttga
     3541 tcacagcagc acttctctta ggatagtcta ctttcacgat ttggataggg gtaggctttt
     3601 tatggataac cgccaacccg cgggaaagat aatatttatt actttcattg attgcttctt
     3661 caaagcgcat cccccgattg ccgaattcag tgatctgatt tcttttttct tgaatttttt
     3721 cgacgggaga ggcattctta taataaggtt ggccatttgg atagcgcaat accatcttta
     3781 atcacactcc taggccacat tatagcaaaa aagcggatag aaagaaaagg aatctgagat
     3841 ggataagtca aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg gtgtttttaa
     3901 agaaaatgat cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac tccatcagtt
     3961 agcagaagaa gggctggaat gggtacttgt cagtgggaat ttgggcgtcg aactatgggc
     4021 tgtgcaaata gtagcggaat tgaaagaaga atatccggaa ctatctcttg gtattttgta
     4081 tcctttttct gattttggta ataactggaa tgaagggaac caagaaaaaa aacaattagc
     4141 agaacaatta gcagattatg tcgaagctgt tagccatcaa ccctaccaat cacctagtca
     4201 gctaaagaat cacactcgtt ttatacttga gcatacagtg ggctgtctgc tgatctatga
     4261 tgaagaattc cctggaaaaa cgaagtattt ttttgaagat gcacagaatt accaagcaga
     4321 tcatccctac gaaattcggc tgatcagtat ggacgatctt caaaatttta gtgaataatc
     4381 gatattgtgt ttgatttatc aacttttttc ctatacaatg aaaaaggtag aaatgttatt
     4441 acgtaaatga aaactagagg tgtaaatatg gcgaatttgg tttatagtcc taaagacatt
     4501 ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt tgatgaattc
     4561 ttagacaata tcatcaaaga ttatgaaact tacagcaaag aactccttgc tttacaagaa
     4621 gaaaacgatc gattgagtgc aaaagtagct caattatcta aaactcaagg agcagctcaa
     4681 acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag tgaccaattt tgatatctta
     4741 aaacgcttgt caaatcttga acgtgaagta tttggtaaaa agcttgatca acaagcaagt
     4801 gcagtcaaac cagcacaacc taatccaaat aactacacaa atgcagacac aagcttagat
     4861 gataatgaaa aaacacgtca attttaattt ttgattaatg aatttgcggt tttcgggtaa
     4921 tcgcggtctg acttgatcag gctgaggaaa gtccatgctc gcacaggctg tgatgcctgt
     4981 agtgttcgtg cttagcgaaa ccataagcta aggtactctt tggagtaacg gcaggaaaac
     5041 aagctaaggc ttcggctatg cttaagtatc cttgaaagtg ccacagtgac gaagcggctg
     5101 gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa acaatggtag
     5161 gggcgctttt tgtctggaag aagaacagga caaaaaggca gtataactgc agatagatgg
     5221 ttaccgcttt gttttttctc cctggaaaag caaagtacag aacatggctt atagaaaacc
     5281 gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt tataagaaac aagaaaaaca
     5341 tttgaaagct atttttcaat acttcagatg aatcgttctt attacaaaac aaacttttgt
     5401 atcaccggtt attcggaatt tgagtaaaag tgtggggtag gactgatgtc ccacctcctt
     5461 tttttataaa taaggagatt gtgtatggaa aaaaacagga agttcaacct cgtcgcaact
     5521 gcggcaagcg ggctagaagc actggttggt aaagaattaa gagatcttgg aatcgaatgc
     5581 caagttgaga acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc tacagctaat
     5641 ttatggttac gtacagcaga tcgagtaaaa atcgtggttg gggaattcga tgcgtatacg
     5701 tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg aagattttct tccactagat
     5761 gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac acctgattgc
     5821 caagcgatca caaaaaaagc aatcgttgaa cgtctccgta cctattatca ccgtccagct
     5881 tctgtaccac tgacggaaac tggcgcacat tttcagttgg aagtagcttt gctgaaagac
     5941 catgtgatgg taactctgga tacgacagga ccaagtttat tcaaacgtgg ttatcgctta
     6001 gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag cacttattat gttgacaaat
     6061 tggcgaaaag accgtccatt ttatgatcct gtatgtggtt caggtacgct ttgtattgaa
     6121 gcagccttaa taggccataa tatcgcacca ggattcaatc gtgagtttgc ttgcgaatct
     6181 tgggattggt tcagtcaaga aatcatggaa aatgttcgat cagcagccga agaaaaagca
     6241 gattatgata ttgaattaga tattactggt tcagatatca atggacggat gattgaaatt
     6301 gccaaagcca atgcagaaga aatcggatta ggctcttcga ttacctttaa gcaacaagca
     6361 gtcaaagact tcaaaacgga taaagaatac ggggtcatcg tggctaatcc gccttacgga
     6421 gaacgtctag gagaagaaga aactgttcgg aaattatata aagaaatggg cgatgtattc
     6481 cgtccattaa aaacatggag taaatatatc ttgactagcg acttagcgtt tgaggaattt
     6541 tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg tacggatcta
     6601 ttccagtatt ggggaactcg tccgcctaga aaaaaagaga aagacgaata atcttactaa
     6661 ttgattcatt caattggatg tttggaggaa tagggaatga aagaaaaaga gtttttacaa
     6721 gaagtaaaag aaatcaatct tctacaacaa gctttaggaa tcctcgattg ggatacacaa
     6781 acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta tctttacagc
     6841 atttattttt caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta tttttctgaa
     6901 catccggaag aattgtcgga agttggaact gccgtttttg ccaaagtaaa agaagagtat
     6961 gatttggaac atcgcgtacc ggaagatctt atgagaaaat atacagctgc cgtttcttca
     7021 gcacacggac aatggcaaaa agctagagaa acacaacaat tttctgattt tcaacacgca
     7081 ttgaatcaaa atattgaatt aacaaaacag ttgatacctt actggagaaa agatgaaaag
     7141 acgaattatg atgttttgct gaatcaatac gaacccggga tgactgtcga aatccttgat
     7201 aaggtatttg atcagcttaa agaaggaatt ctttcgatcc gccgtacgct ccaagaaaaa
     7261 gggactgaac ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca acagaagaga
     7321 tttgtagtga aagtaatcga acaacttggt tatgatttta gtcgtggtcg tttagatgac
     7381 actgtccacc catttatgac tggaattaat ccgaatgatg cacgaatcac gacacgatgg
     7441 aacgaaacag atttcaaaat ggctgttttt ggagtgatcc acgaggctgg tcatggtatg
     7501 tatgaacaag acatcgatga taaatatgcg tatacaccgg tttatgaagg tacatcaatg
     7561 ggtatccatg aatcacaatc gttgtttaac gagatcatta ttggaagtag tcgcagcttc
     7621 tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag gaacattcga tgatattcct
     7681 tttgacaaat tttatcgttc attgaaccat acaaaatcaa gtttggttcg tatcgaggca
     7741 gacagtttga cttaccctct ccatatcatc attcgttatg aaatcgaaaa aatgattttc
     7801 aatgaagggg tagatgttgc tgaactacct gaaatatgga atcaaaaata tgaagagtat
     7861 ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac aagacgttca ttggtcaggt
     7921 gcaagctttg gttattttcc ctcttatgca ttagggtaca tgtatgcggc acaattactt
     7981 cattctttgc agcaagaaat agatgtggaa aatgttctag cgtcagacga ttattctcca
     8041 atcaaaaact ggatgtcaga gcatatccat caatatggtg catctagaaa accaaatcaa
     8101 ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat acctgatcga ctatatgaaa
     8161 gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga tagctgaaat
     8221 tcatctaatc tatagatgtt ttcagctatt tacttaagaa agaagtggaa cgaatggata
     8281 aattacaagt gatcgctgat tatagtatgg accaattagc gcatgatcaa acaggacatg
     8341 ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa aattttggag acggaaccgc
     8401 aggcagaccg ctttgtgacg cttgcagcag cttacctaca tgatacgatt gatgataaag
     8461 tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt attccttcgc tctttgccaa
     8521 tagtagagga acagatctcg atgatttttt ccattatcga aaatatgtcc ttttctaaaa
     8581 atctctctga agcggccgaa ctatctctgg aaggtaagat cgttcaagac gctgatcgta
     8641 tcgaagcatt aggagcaatc ggcattttgc gtactgctta tttcggcggt ggacatgggc
     8701 acccgatttt tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa aattaccgaa
     8761 aaggcacgac agtgatcaat catttttatg aaaaactgtt cttgctgtct gataaaatga
     8821 atactgatta cggatatgaa gaagccaaac gaagagaaac cttcatgcgt gaattcttag
     8881 aagaattttt tctggaatgg tctattgacg atgaaaattt tactccagac aattggatca
     8941 aaaataacaa aaaatgatgg aggttgtgac agaagtggtc agctccaaga tatttcggat
     9001 cattttccgt aaaagttact tctggaacac tgtttaccaa gaggtcgtga gcctgccgtt
     9061 cagctccgag tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga tgaatattgt
     9121 aacttgtgat tcggaattgg caggagaaca ccgtttatt
//
LOCUS       DAZKCC010000103         8485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000103 DAZKCC010000000
VERSION     DAZKCC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.105"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..500
                     /locus_tag="ACG8VH_002624"
     CDS             <1..500
                     /locus_tag="ACG8VH_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111126.1"
                     /translation="SIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGK
                     KETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFE
                     KTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLV
                     EYGII"
     gene            487..1908
                     /locus_tag="ACG8VH_002625"
     CDS             487..1908
                     /locus_tag="ACG8VH_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9111127.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1921..2928
                     /locus_tag="ACG8VH_002626"
     CDS             1921..2928
                     /locus_tag="ACG8VH_002626"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFP9111128.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2940..3944
                     /gene="neuB"
                     /locus_tag="ACG8VH_002627"
     CDS             2940..3944
                     /gene="neuB"
                     /locus_tag="ACG8VH_002627"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFP9111129.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3941..5089
                     /gene="neuC"
                     /locus_tag="ACG8VH_002628"
     CDS             3941..5089
                     /gene="neuC"
                     /locus_tag="ACG8VH_002628"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFP9111130.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5062..5739
                     /locus_tag="ACG8VH_002629"
     CDS             5062..5739
                     /locus_tag="ACG8VH_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9111131.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5690..6871
                     /locus_tag="ACG8VH_002630"
     CDS             5690..6871
                     /locus_tag="ACG8VH_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9111132.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            6884..7861
                     /locus_tag="ACG8VH_002631"
     CDS             6884..7861
                     /locus_tag="ACG8VH_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9111133.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            7861..>8485
                     /locus_tag="ACG8VH_002632"
     CDS             7861..>8485
                     /locus_tag="ACG8VH_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9111134.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATY"
BASE COUNT         3078 a         1066 c         1548 g         2793 t
ORIGIN      
        1 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
       61 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
      121 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
      181 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
      241 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
      301 tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag
      361 cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata
      421 accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg
      481 tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg
      541 tatactatag ggaatttttt tattaaggga atttcattca taactattcc tatttttgta
      601 aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt
      661 ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat
      721 aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt
      781 gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac
      841 caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc
      901 cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct
      961 acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa
     1021 tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat
     1081 ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta
     1141 aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat
     1201 agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt
     1261 aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg
     1321 ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat
     1381 ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa
     1441 tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc
     1501 tatttctttc agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca
     1561 tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt
     1621 ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta
     1681 ctttttattt ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca
     1741 aaaaaaatat gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta
     1801 atcaatcaaa tgttgattcg ttacgttttg acatcattat tgataattag tttagttata
     1861 tattttttaa agattaagaa tcgaattata gatgactata attcatagga ggagatataa
     1921 gtgtttaaag gaaaaacaat attagtaact ggcggaactg gttccattgg aagtgaaatt
     1981 gttcgtcagc ttttaaaata tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa
     2041 aaacaatttt atatgcaaca agagctctca aatcacacta atttgagatt tttaattggt
     2101 gatatcagag actctcaaag gattgattat gcatgtcgtg atgtggatat tatttttcat
     2161 gctgccgcaa tgaaacacgt tccagcaagt gaatataatc ctatggaagc tgtaaaaaca
     2221 aatgtgatgg gaactcaaaa tgtaatagat gcagcgatca aaaataatgt aaaacatttt
     2281 gtaggtatca gtacagataa agtagtgagt cctactaaca ccatgggtgc gacaaagtta
     2341 ttgagtgaaa aattaataat agctgccaat cagtataaag gaaaaacaga aacaatattt
     2401 tcttgtgtaa ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc tctttttagt
     2461 aaacaaataa aaaacaataa agatattact ttgacagatg ttgatatgac acgatttatg
     2521 atgtcaatac cggatgctgc aagattagtt ctgtctgctg ctatgaattc aaaatttgga
     2581 gaaacatatg tcttaaaaat gccggcaatc aatattttaa ctttagctca atcattaata
     2641 aaaatatata atgatacaca tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc
     2701 aggccaggag aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat
     2761 gaaaatgata aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa
     2821 gattatgaaa aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga
     2881 caggaattta ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga
     2941 tgaaatcaga agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt
     3001 tagctttaga aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa
     3061 catttaaaac gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata
     3121 atcttaaaga ggacagttct caatttgaaa tgctaaaaaa attagaatta tctcaatctg
     3181 attttattag aataaaagat tattgtatac aaaataaaat tgattttttg tctactcctt
     3241 ttgatgaaga aagtgcagac tttctaaaac aaattggtgt acaaggattt aaaattggct
     3301 caggagattt aacaaattta ccttttctta gaaaaataga tatgtatggg ttgccagttt
     3361 tattatctac aggtatggct gatattcatg aggtcagtga ggctgtagat tgtttcatag
     3421 atagtccagt tactatttta cattgtactt caaattatcc ggctagttct gaagatatta
     3481 atttattggc gattaacaca ttaaaagaaa tgttcgggat acccgttggg tactccgatc
     3541 attcattggg atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga
     3601 aacattttac cttggataag gaactacctg gtccagatca taaagcttct ttagatcctc
     3661 aggaatttgc agattttgta aagcatatta gaaacactga aatattttta ggtgatggtg
     3721 ttaagcgagc aatgccttct gaacttagta caaaagaagt atcaagaaaa agcctagtgg
     3781 tcactgagaa tttaaatgtg ggagatattc ttacagagaa atctcttaca ataaaacgtc
     3841 ctggaaatgg aatagctcct aagtttttat atcaatatat tggcaaaaaa gttaaaagac
     3901 caataagtgc agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa tacttatagt
     3961 aagtggaaca cgagctgatt atggaatata taaacctgtt gctgagaatt tactacaaga
     4021 taattgggat gtatcatttg ttgtttctgg aatgcattta tcccataaat atggatttac
     4081 taaagaattg atcttagctg acggatttag aattagtgga gaagttcaaa gtttgttttt
     4141 agagaacaat aagggaaaca tggctcggtc tgtggcatta gagataaatg gtttcacaaa
     4201 tatatttgaa caaactagcc ctgattttgt attattacta ggggatagag gagaaatgtt
     4261 ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca gcacatattc acggaggcga
     4321 agtgtctggt actgtagatg aatcaataag acatgccata acgaaaatag catctgttca
     4381 ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa atgggggaag attcttggag
     4441 agtttttccc gtcggagctc ctagaataga tactatttta aataatagat tacctagttt
     4501 tagagatata aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg ttttgcaagt
     4561 atttcatcca gttgtaaccg aactagaaaa tatagaaaaa caaattttag ctttgatcaa
     4621 ttcaatggaa caacaatctc aaccaattat ttgcatttta cctaatagtg atgctggtag
     4681 cgaaattatc agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa
     4741 tttagagccg gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc
     4801 ttcttctggg ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag
     4861 acaaaatgga cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat
     4921 tgaaactgct ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa
     4981 gaacatctac ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt
     5041 gaatgatggg gaagatatta agtggattca aaaacaaata gcttattaaa atatactagt
     5101 gatccataca ttattattgg ggctggcgga cacgcaaaag tcattattga aaccttaaga
     5161 ttagaaaata agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg
     5221 aaatatcttg ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat
     5281 aaatttattg tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat
     5341 atgccaagtg agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt
     5401 tcaattggtt atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt
     5461 ggagagcatg ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac
     5521 tatgttcatt tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact
     5581 caaataggaa ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata
     5641 ggcgcaggga gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca
     5701 gctaaaatta agaaagaagg aattaatttt gaattctaga attttattat caagtcctca
     5761 tatgggagga acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc
     5821 attaggtgaa aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg
     5881 cggagcttct gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa
     5941 tgttggaaaa aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc
     6001 tattttatat caaggggctg taccagtgtt cattgactca gaacctaata catggaatat
     6061 gagccctgtt gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa
     6121 ggcaatcatc gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat
     6181 tgcaaaccaa tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta
     6241 taagggaatt tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa
     6301 gatcatcaca acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca
     6361 ttcaagattt ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat
     6421 aggttataat taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt
     6481 attgaatgat agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc
     6541 tggaattgat agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt
     6601 gtctacttta acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa
     6661 actaaatgaa aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt
     6721 atttcaaggg gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt
     6781 taatagcggc ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt
     6841 tattgatgag tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat
     6901 tggtttaggt tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat
     6961 tttactatgt ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg
     7021 aataccaact tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt
     7081 tgtttgtaca tcccctataa cccatgaatt tattataaaa gaggctttaa atttagattg
     7141 taatatcttt acggaaatca atttattaaa tgaatattat gatgaaataa tcgaagtagc
     7201 taaaaagaaa gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca
     7261 atatattaag tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca
     7321 gtatttaccc gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa
     7381 aactaatggg tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg
     7441 gaaagttaaa tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc
     7501 ggacagtttc actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga
     7561 tgttgtttca agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga
     7621 atgggaaggt aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga
     7681 tattcaattg tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga
     7741 tgcatattta gaggagatta aagaattttt cacgcttttg gaaaagaaaa tggaacccca
     7801 atatactttt gaaaaagata agtatattat taacttaatt aacgacattg aggtaaatta
     7861 atgatgaaag taattttttt tgggttaggg tcaattggaa agagacatct aaaaaatcta
     7921 ataagctatg gaaaaaagaa ttctattaat tttgatataa cagcttatag tagcagtgga
     7981 aaaaaagagt ttgaagaaat aacgtatatt caaaatagag acgatttgga tacacattat
     8041 gatatagctt ttattacaaa tccgactttt aagcatctgg aaacactaaa tgtaattgtt
     8101 gaaaaagttg attacatatt tttagaaaaa cctgcttttt ctgagagtat tgatcttact
     8161 caatataatt atgattacaa aaatatttat gttgctgctc cactgaggta taaaaatatc
     8221 atggaagttg ttcgtaatag tattcaaagt aaaaaagtat attctgtaag agctatttgt
     8281 tctacttatc ttcctgattg gagaaaaggt actgattata gaaataatta ttctgccttt
     8341 aaatctatgg ggggaggagt agaattagac tgtatacatg aattggatta tattataaac
     8401 ctatttggtt tccctaaatc agtaaaaaaa cagtttggta aatattcgaa tcttgaaatt
     8461 aacagtaatg atttagcaac atata
//
LOCUS       DAZKCC010000104         8428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000104 DAZKCC010000000
VERSION     DAZKCC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.106"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8VH_002633"
     CDS             476..1339
                     /locus_tag="ACG8VH_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9111135.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8VH_002634"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8VH_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9111136.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8VH_002635"
     CDS             3339..3860
                     /locus_tag="ACG8VH_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9111137.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8VH_002636"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8VH_002636"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP9111138.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8VH_002637"
     CDS             5941..6759
                     /locus_tag="ACG8VH_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP9111139.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8VH_002638"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8VH_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111140.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8VH_002639"
     CDS             7228..7806
                     /locus_tag="ACG8VH_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9111141.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8VH_002640"
     CDS             7901..8038
                     /locus_tag="ACG8VH_002640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111142.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2548 a         1509 c         1649 g         2722 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactg
//
LOCUS       DAZKCC010000105         8248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000105 DAZKCC010000000
VERSION     DAZKCC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.107"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACG8VH_002641"
     CDS             234..1559
                     /locus_tag="ACG8VH_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9111143.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACG8VH_002642"
     CDS             1552..1902
                     /locus_tag="ACG8VH_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111144.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACG8VH_002643"
     CDS             1899..2069
                     /locus_tag="ACG8VH_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111145.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACG8VH_002644"
     CDS             complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACG8VH_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFP9111146.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="ACG8VH_002645"
     CDS             complement(3220..4173)
                     /locus_tag="ACG8VH_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9111147.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="ACG8VH_002646"
     CDS             4785..6278
                     /locus_tag="ACG8VH_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9111148.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="ACG8VH_002647"
     CDS             6413..6709
                     /locus_tag="ACG8VH_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP9111149.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="ACG8VH_002648"
     CDS             6810..7661
                     /locus_tag="ACG8VH_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111150.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7894
                     /locus_tag="ACG8VH_002649"
     CDS             7682..7894
                     /locus_tag="ACG8VH_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111151.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2864 a         1283 c         1552 g         2549 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtaaa cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DAZKCC010000106         8013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000106 DAZKCC010000000
VERSION     DAZKCC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.109"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..747)
                     /locus_tag="ACG8VH_002650"
     CDS             complement(<1..747)
                     /locus_tag="ACG8VH_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9111152.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIF"
     gene            complement(867..1514)
                     /locus_tag="ACG8VH_002651"
     CDS             complement(867..1514)
                     /locus_tag="ACG8VH_002651"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFP9111153.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1598..3025)
                     /gene="uxaC"
                     /locus_tag="ACG8VH_002652"
     CDS             complement(1598..3025)
                     /gene="uxaC"
                     /locus_tag="ACG8VH_002652"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9111154.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3099..4178)
                     /gene="uxuA"
                     /locus_tag="ACG8VH_002653"
     CDS             complement(3099..4178)
                     /gene="uxuA"
                     /locus_tag="ACG8VH_002653"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9111155.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4181..5812)
                     /locus_tag="ACG8VH_002654"
     CDS             complement(4181..5812)
                     /locus_tag="ACG8VH_002654"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9111156.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6116..6787
                     /locus_tag="ACG8VH_002655"
     CDS             6116..6787
                     /locus_tag="ACG8VH_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9111157.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIGLATHPK
                     YFKNI"
     gene            complement(6947..7138)
                     /locus_tag="ACG8VH_002656"
                     /pseudo
     CDS             complement(6947..7138)
                     /locus_tag="ACG8VH_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(7506..7847)
                     /locus_tag="ACG8VH_002657"
     CDS             complement(7506..7847)
                     /locus_tag="ACG8VH_002657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111158.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT         2626 a         1558 c         1067 g         2762 t
ORIGIN      
        1 gaaaatagcc gcaccaatta tagaaagtaa tgccctattt ttaactaacc cttttaaaga
       61 ttttaacaat gaaactttgt attgagattg gtcaattctt actcgttcag tagttagttt
      121 ataacatatt aaataacaaa taagtgcaca tatcgaaaat attcccgcaa caattgtaaa
      181 agttgatcca ccacgaacta tttgagcgcc tgaatcatct cttgtataaa taaataatgg
      241 tgttaataca ccaatgacca aacttgctaa agtagctcca acatttcgga atgtcgatag
      301 cattgttctt tcttctgtaa tagaagtaat agctgatgcc attgatccat aaggaatatt
      361 tatagatgtg taaaaaatac tcccccataa tatatacgta acatacatgt atactacctt
      421 aacgctcata gaagctgaaa tcatagaagt ttgatacatt aaaaaacttg ctaaagcgac
      481 tggaccactc attcttaaaa tccacggttt aaacttaccc tgcttagttg ctttcgatct
      541 atcaacaata cgccccatag ttacatcagt aactgcatcg ataaatcttg ctactaaaaa
      601 taatgttcca acggcagctc cgcttatccc caatactttt gtataaaaga tcattaaaaa
      661 agaactagca aaaatgaaag taaaatcgtt tccaaaatct ccaaacatat atcctatttt
      721 gtctcttata ccaaattttc tttccataca attctctccc ttctgttgtt aagcgttttc
      781 attctgatga aagtacgtac tatactctga agcgtaaaga gtcagtattt attttttaca
      841 tttcagagta ttatttgttt tttctcctaa taattcttta tctcataaaa cttatctata
      901 tatttactag ctactcgact tacttcttca aaatcacctt tttcaacaga agccaataaa
      961 ttcccaccta caccaactgc aacaactcct acttcaaacc aatcttctaa gttctctata
     1021 cttactccgc cagtaggcat tatatttacg tgtggtaatg gagcttgata ggcttttaca
     1081 atggatggac caaaagcaga acctgggaat agtttaatga tatctacgcc agcagtaagt
     1141 gcctccttta tctcagtaat tgtcatacaa cctggtaaat aaggaatttg atataaatta
     1201 caaatctcag ctgtttgtcg atcaaaagat ggactaacga tatattctgc cccagccata
     1261 attgcgatac gagcagtaaa cgcatctaaa accgtaccgg ctccgattat tacatctttt
     1321 tgatcagcat attttttagc aatttctttg attatttctt cagcattagg aactgtaaaa
     1381 gttagttcaa tccctttaat tccacccgca ataattgaat ttattgcttt aatggcctca
     1441 tccttatttt tcccacgtac aaccgcaatg actccagccg cttctaactt acttaaaatt
     1501 tctacttttt tcatatctat ctccttcctt tatggataca tactttgtct agctgtacta
     1561 acttacaaag aataaattta agcttttatt tcaagtatta ttttctactt ccgatttcaa
     1621 aaaaattaaa ataattatac gcattacgat aacaaatatt ctccactatc tgactaagat
     1681 acatttcatc atctggaatt cggcctgctt taacccactc gcttagtaat tgacataata
     1741 ctcgacgaaa atactcatgg cgaggatagc ttagaaaact tcgagaatca gtcaacatac
     1801 ctgtaaaatt cgacaacaaa ctttgttgtg ccatcgtagt caactgttga cgcattcctt
     1861 cagctgtatc attgaaccac caagcacatc ccaattggat tttttgaatc ataccccctt
     1921 ggaagctttg cattgctgtt gctagtggaa gccaatcagt tggatttaat gaatataaaa
     1981 ttgtttttgg taagcaccca ctcttttcag cggtatcgaa caatgctaca acattttgtg
     2041 tcaaactagc ttgatccccc atcgaatcac caccgacatc tactccaatt tgttccttta
     2101 atatacttga attatttctt aaagcattta aatgcatttg catcgtccaa ccatatcttg
     2161 tatactcaga catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat
     2221 ggaaatccgt ttttcctgct aaagctttta ttaacagatt gtcgagtatt ttttcatctg
     2281 ctatttcata aaaataagta ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa
     2341 aatgatccac tcgttggctc aatgcttgct tcaacccatt aaagtctttg attggtatag
     2401 ccgctgtttg actaagtttt aaaatatagt tcgaaaagcc tgatgatttg atactccaga
     2461 ccttatctgg acggaaagta ggtaatacct tgaaagaatt atcctgttta gaaagctcag
     2521 tatgatacca caagtcatct gtaggatcat ccgttgtaca gatggctttc acatccatca
     2581 tctccaacaa ttctttcggg cgataggaat ccgtttgaat tttttcgtta gcacgtcgcc
     2641 aaatttctgg tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt
     2701 ccaaatgaga ccattcaaaa ataggattac ctggcgcctt ctccactgtt tttacaaaag
     2761 ctaaaaattt ttcataatca tcacctggac cactgatcaa ctcttcttct ataccattag
     2821 ctcgcatcaa tcgccattta taatgatctc cagctccttt atcatttaac catacctgag
     2881 tcaaattctt gtattgctta ttttctagca aatatgctgg gtctaaatga caatgatagt
     2941 cgataattgg ttggtgtttt gcaaatttat gaaaaagtct tttagctatt tcattctcta
     3001 ataaaaagtt cttatctaaa aacatattta aacactcctt tccttccgtt agtttttaga
     3061 agagaaaatt tttcctctaa ataaactccc ttatataatt atttactcgc ccccttttca
     3121 agcgcttccc aaataccatt taaataaact gcacctagag ctcggtcata taatccataa
     3181 ccaggtctac cggtttctcc ccaaatcatc cgaccatgat ctggacgaat atagccatta
     3241 aatccattat cgtacattgt ttttaatatc ttatacatat ctagagaacc atattctgat
     3301 ttatgcggcg cttcgtaaaa atcgagtcca ttgtcactta aatatttgat attacgaaca
     3361 tgtgcaaaag gaatccgatc ccgttgacaa aattcagata gaatttcata gacatcatta
     3421 tctggtcgtt ctgcaatact tccagtacat aatgtaatac cattagaagg agaatccact
     3481 acattacaca accaatctaa atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg
     3541 gaatatggtg gatcatctgg gtgaactgcc attttaatcc caacttcttc acatgtcggg
     3601 ataattgctt tgagaaaata tgcaaagttc tctcgtaatt tctcttcatc aacttcttta
     3661 taggcttcta ataattgctt aatttgtgat aaccgatgag gttcccaacc cggcaattca
     3721 aaattattag tagaattttc tacttctcta ataatttcct ccggatcagt agggatattt
     3781 tttgaaatat aggctaacgt agaagaccca tcatctaaac gataatccaa atccgatttt
     3841 acccaatcaa atactggcat aaaattataa cagataacct caatcccaaa ttctgccaaa
     3901 ttgcgaatcg tctgtttata attctcaatg tattgatctc gtgttggtaa accaattttg
     3961 atatcatcat gaacattaac actctcaata actttcaagg taagtccatt ggcttcgact
     4021 tcatctttta aagatttaat tcgttctctc ggccaaacct ctccggctgg tatatccatt
     4081 aatgttccaa caattccttt cattgctggg atctgacgaa tttcttgtag tgagattttt
     4141 tcttcatttg ccccatacca tctaaaagtc atttccatag ttactttcct ccaacttctg
     4201 ttatatattt attgattgtt ttacgaactg cttgaggacc tactagcatt tcgcaaaaga
     4261 ttttttcagt tttctttcct accccaactt gatataaatc taaaccaaag atttctttat
     4321 tttctaaaat ttcctgtacc gcacaatgaa tctttgaagg atcttgttca cctaatttca
     4381 atactgataa tttatcctgc aactctacca ataacggatc aggacttggc acaaaatttt
     4441 ccccttgatc atcgactgcc agtaaataac gtaaccaagc agcaatagcg ataggaataa
     4501 atgttagatt ttctgcattt ccgtcttttt cgacatactt tttaattgtt tcaccatagc
     4561 gaatcgctac tttttgagaa gtatctgttg caattcgttg tggtgtatct ggaatatagg
     4621 gattaggcaa acgtttatta attacttcat caataaattg ttttggatca ataatgcctg
     4681 gattttcaac tactggtaaa ccttctaaat aacctattcc tttgattaaa ccaaccaaat
     4741 catcatcctg catctctgct gaaattgatt gatatcctag cagacaacct gttactgcca
     4801 aagcagtatg aagtggattc aagcttgtcg taaccttcat gatgtctgct ttatcaaccg
     4861 tctctctatc tgtcataatc acaccagcct tttccaattt aggccggcca tttggaaaac
     4921 tatcttcaat aactaaataa tgaactacct cagtattagt gaaagcagca atattggtat
     4981 gtttttttgt gtgaatgata tctaaatcct cgagccccat catcaataga tcttccttta
     5041 cattctctga aggatttggc gtaattcgat caatcatact ccaaggaaaa gtcacttttt
     5101 ctttatcctt taaataagca ataaattttt cagaaacata tccttctttt tgccaagctt
     5161 ctgcaatcgt aaaaatacta tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag
     5221 aaaccatcgc aatcggcaac tctccagcat taaatctgct atataacaag gatgttacaa
     5281 tactcatcgt atgttgagga ttgctaggtc cagatttgat atcagctttg actaccgaaa
     5341 agaattgccc tttagaatct tttaaactgt aacctttttc agtaatagta aacgttgtaa
     5401 actgtaattt aggatcttca aaatatttaa ccactctttg atagtccgct atattcttcg
     5461 gattgcaata taaactatca gccgttactg ccaaaagtct ttttttgagt tcaccatctc
     5521 cactcataac aatttccaaa atgtcattgt tatagtcatg ataagctttt tccactactt
     5581 cttcatcaaa tgtttcacac acaactactc ctgtttctaa ttcattttga tctgctaagc
     5641 attgagcaat ttctgcatga aatcctcgat aaagatttcc tccaccaaaa tgaagccata
     5701 ctggatgttg ctggccttct tgcttaactt ctttgacatc aaaacacggc agtagaatct
     5761 gcatttcatt gaattcttcg atagaatttt gccaattttt aatcgaaacc ataattaacc
     5821 tcctcaaact gatatgttag ttttgataca ttttatattt taaaacccat tgtaaccgtt
     5881 gtcaatataa aaagggaata aacttgcttt tttctaccat atcagaatga ttgtattttt
     5941 tattcagaaa aataaaatac tccttctttt tggactgatt ttatcgaaac ttattttttc
     6001 tttttttcat gtattctttt atttttataa ctagtgtatt acttcaaaat ttgatattat
     6061 atggcatgaa cagaattaaa atatcagtta aaataaaagt taaggagcat tgtgtatgac
     6121 aattaaaact agtctccaaa atcaagctta ccaatctatt cgccgacaaa ttatctatgc
     6181 tgacttagaa cctggaaaaa aagtatcaga aaaaaaatta gaagaaacac tttctattgg
     6241 tcgtacacca atacgtgaag cactgattca actccgacaa caaggtcttg ttgatacaat
     6301 tcctcaaagc ggaaattata tctcattaat tgatttagat tcggccaaaa atgctcgatt
     6361 tgtcagagaa catttggaac gacaaattat gttagagtgt tgtgccaaaa tgaataacca
     6421 aaaaaaacgg attttagaaa ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa
     6481 tgctcgtagt ttttttcata atgataattt attccataaa gcttgttttg aaattgcaga
     6541 gcgagaagaa gtatggcaat ggttagaaaa taacaataca cacttagaac gatttcgatg
     6601 gctacgagta aaaacggaag gattaaagtg ggaaaaaatt atggaacaac attatcaact
     6661 atttgaggca ttaacaaaac aagatccaga agaagctaac tttctaacat cgttgcatct
     6721 tcatttaatg cttaatgagc aaaatattgg tttagctact catccaaaat atttcaaaaa
     6781 tatataactt ttaactcttt ttaaattaaa tggttgttct gatcagaaaa atatcgcttg
     6841 ttttcttatt atgtaatata tttcataaac tattctgcat aacaactatt gtttgtactt
     6901 tttacattgg ataactaatt atgactgctg ataacttttg atattaaact agtatctcca
     6961 atacaaataa aaaatagata tcttgttcat caaaattagt tcgtggatac ttaccttcat
     7021 ttacatcatt cagcggtttc ccattttctt ttattctatt atttcatttt ctaatagact
     7081 tggcttcatt tcgcaatact ttttttcaat aggcatcaaa ttggtgttgt ttagatattt
     7141 agttgtagtt ttgtgacatg ataagtatgc tcctttttct cttttccact aatacaacga
     7201 tcaaagggtg cttttttcaa aaaactagaa aaatcaaaat aagattcgta ttttgattct
     7261 atatatcctg tttcaactac atcttatatt tcacaataga ataaatagcc ttttgcatag
     7321 ctgcttaact tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa
     7381 ataaaattat tcttcttaca gataattaaa aatagcgcgt aacaaaaatc ggcattcaat
     7441 cacataatta ggattgatgc cgattactta tattctacgt atagttttag tattcttatt
     7501 ttttatcacc agtaaccaat tgagaacagc cctaaagcag aatagtcttt tatctcaaaa
     7561 cgcttaattt caggcttagg aatatgaata atcttttgag tatattcttt ctcttttaat
     7621 cttactgaag aaataccact gcctatcttt tttacaaata tttcatttgg tgtacagact
     7681 aataaaacaa tatttgtaat caatccttgc actaaagaat taaaaggaag actatttaaa
     7741 acagggtgtg cataactata acatgtgaag tagttatttt gaactttaac ttttaaaaac
     7801 tttaaaaata atcttatttg ttcttctaaa caatcatcta tactcatata atcactccac
     7861 taattaatct ccccaaaaag acacagcatt aagagatata ccaacacaac agtgcaaata
     7921 actctacatc ttccttcgca accttatttc aataaaccat aaaaattatt ttttaataga
     7981 aactgaaaat taaaactaga aaaagtagtt tta
//
LOCUS       DAZKCC010000107         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000107 DAZKCC010000000
VERSION     DAZKCC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.110"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8VH_002658"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8VH_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9111159.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8VH_002659"
     CDS             1483..1935
                     /locus_tag="ACG8VH_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111160.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8VH_002660"
     CDS             1949..2638
                     /locus_tag="ACG8VH_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111161.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8VH_002661"
     CDS             2763..5114
                     /locus_tag="ACG8VH_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP9111162.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8VH_002662"
     CDS             5194..5589
                     /locus_tag="ACG8VH_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111163.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8VH_002663"
     CDS             5914..6531
                     /locus_tag="ACG8VH_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111164.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8VH_002664"
     CDS             6572..6871
                     /locus_tag="ACG8VH_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP9111165.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8VH_002665"
     CDS             6905..7072
                     /locus_tag="ACG8VH_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9111166.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8VH_002666"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8VH_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111167.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKCC010000108         7222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000108 DAZKCC010000000
VERSION     DAZKCC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.111"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..526
                     /gene="rpsP"
                     /locus_tag="ACG8VH_002667"
     CDS             251..526
                     /gene="rpsP"
                     /locus_tag="ACG8VH_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9111168.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            538..789
                     /locus_tag="ACG8VH_002668"
     CDS             538..789
                     /locus_tag="ACG8VH_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP9111169.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            914..1705
                     /locus_tag="ACG8VH_002669"
     CDS             914..1705
                     /locus_tag="ACG8VH_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111170.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1875..2396
                     /gene="rimM"
                     /locus_tag="ACG8VH_002670"
     CDS             1875..2396
                     /gene="rimM"
                     /locus_tag="ACG8VH_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9111171.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2386..3147
                     /gene="trmD"
                     /locus_tag="ACG8VH_002671"
     CDS             2386..3147
                     /gene="trmD"
                     /locus_tag="ACG8VH_002671"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9111172.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3283..3630
                     /gene="rplS"
                     /locus_tag="ACG8VH_002672"
     CDS             3283..3630
                     /gene="rplS"
                     /locus_tag="ACG8VH_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9111173.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3762..4898)
                     /locus_tag="ACG8VH_002673"
     CDS             complement(3762..4898)
                     /locus_tag="ACG8VH_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9111174.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(5014..5580)
                     /locus_tag="ACG8VH_002674"
     CDS             complement(5014..5580)
                     /locus_tag="ACG8VH_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFP9111175.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(5716..6369)
                     /locus_tag="ACG8VH_002675"
     CDS             complement(5716..6369)
                     /locus_tag="ACG8VH_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111176.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(6419..6847)
                     /locus_tag="ACG8VH_002676"
     CDS             complement(6419..6847)
                     /locus_tag="ACG8VH_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9111177.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(6852..>7222)
                     /locus_tag="ACG8VH_002677"
     CDS             complement(6852..>7222)
                     /locus_tag="ACG8VH_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111178.1"
                     /translation="LVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSENLK
                     KVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRVKD
                     ILIATFWDIVKEDKEKGKKM"
BASE COUNT         2236 a         1317 c         1314 g         2355 t
ORIGIN      
        1 gatttttcct ccttcaaact atctataata atcttacaaa agataagtaa ctggagcaaa
       61 taaaaacact tactgacaaa agctacatag aagatagatt acttattttt aacaaaaaag
      121 aagtctaaaa tcttctattt tctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa
      181 agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac aatggaggtg
      241 gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt
      301 ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca tcgaaactgt
      361 aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag acttagttct
      421 tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc tttcaaaaga
      481 aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg accggatatg
      541 aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta tcctgatcaa
      601 gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac agtggctcct
      661 gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc
      721 gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag
      781 gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg ttttttttat
      841 taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta taggaggagg
      901 gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat tgttaagcgc
      961 ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta tatctgaaac
     1021 tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc accactgaac aaacaatggc
     1081 tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac agccaaatgt
     1141 tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag cgacacatac
     1201 aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt tgaatgctga
     1261 cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt ttgcttctag
     1321 tcaagaaaca atccaagttt tgcagccatt tgagatcgac acaaatggtc agcaagtgga
     1381 cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg
     1441 gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc aagatccctt
     1501 actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag cacctgaaca
     1561 atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag aagtaagctg
     1621 gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg cgttgaagat
     1681 ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat catttaactc
     1741 caagtcacag gaagtaatca aaaatagcac gccgcatttt tgcataattt tggcttaata
     1801 ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag acaaaaaaga
     1861 aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac acacaaggta
     1921 tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt tacaaaaaag
     1981 gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca gtcaaaagtc
     2041 atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct atcaatgatg
     2101 tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta gatctagctg
     2161 aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat ggaactgagt
     2221 taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc
     2281 cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa gtcgatttag
     2341 aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa gattgatgta
     2401 ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat cggtaaggct
     2461 gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc agataacaaa
     2521 catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct caaagtccag
     2581 cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa aaaaagagta
     2641 attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga attttcacaa
     2701 gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg tatccgcaca
     2761 ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga attaggtgcg
     2821 atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa tcagacttca
     2881 gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac tcgcccggca
     2941 gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa attgattgaa
     3001 gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga tcttttggaa
     3061 acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa agaagaacaa
     3121 gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca gtaggttgat
     3181 aatatgttag tattttatgg tgagtgcaaa agcacttaac tattacgata ttccgctgtg
     3241 agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc attaatcgaa
     3301 gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg tgacactgtg
     3361 cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt
     3421 gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa agtttctaac
     3481 ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa aatcgaagta
     3541 gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa
     3601 gctgctcgta tcaaagaaat ccgtcgttaa tcatcgttat cagacagaga aagaaccctt
     3661 ggtattcaag ggttcttttt ttattatctg tttttttata ttaacgattt ctatttggta
     3721 catttttgat taataaaaag gggcaaaaaa ggggcaacaa gttacagttt ttctatatct
     3781 tctttcaatt tttcttttac tccttttgtc acatgcaaat aaattttttc agtgatatca
     3841 ctattttcgt gaccaacgcg atcctgaatg gcatacagag gagttcctag ttccgctaat
     3901 tttgaaatat gggtatgtct aaaaatatgt gaactaagtt ttttatcaat cttcatgtca
     3961 gctttatgac ttctcaaata ggtgttaatt gctgttagtt ggaaaggagt tcctttcgtt
     4021 gtttgaaata gaaattgacc atttggattt aattctagaa gctcattgta aatagctatc
     4081 gctttttttg gtaaatcaat ttctcgcatt ccggcagctg tttttgtaga atcagatttt
     4141 ttcatatcag ctattgaacg ttctctatac atcatcgtcc catttataac tactgatgca
     4201 ttattattgg taatgtgtac atcatcttta cttaatgcga ttgcttcacc agacctcatc
     4261 ccagtcaaat atagccactg gaaaaggaga gaatatcttt tattgtgtga agttgtgaaa
     4321 tctactagtc tattatattc atcatcttct aaaaatttat ctttaatttt tatcgttttt
     4381 gactctcttt tataatcaat aactacttca tcgataggat ttttttctac ataacctttt
     4441 ttcatagcat atgaaaaaag aagattcaat ttggatttaa ttacactgac gtacttattt
     4501 gacaagtcat ctttatatat catgtcttca aaaatattat tcaaatctat tgtattaata
     4561 ccagaaacga tgtaagtctc aggtatcttc ttctttatag tgtttaatat attattcgta
     4621 ggataatatg tagattcctt aacttgtctt ttgtatataa ctaaccattc ttcaacaagc
     4681 tcatgaaatg ttagatcagg cttatgaagt gttttttcgt ttaattttat atcaattttt
     4741 ttatttaact ctagcaatgc cactttctga gtttctcgag atttattttt ataagtaatg
     4801 ctcacttttt ttctttttct agtttttgga tcaatatatc gctcattata tttatatact
     4861 ttttttccgt ttttatcagt tttagtttcg atccacattg ttttcatctc ctatttgttg
     4921 ctataatagg aatagataag taagccgatt atagcaggtt tatttttcat cacgtccaca
     4981 aactttggcg agggaggggc gtgtttttgt tagttattgt ggtaggttat ctaaagcgta
     5041 ttgtgcttgc tcatttgtaa aaccttcaaa tataagttga tcgtacaatt gcgaatctga
     5101 cattgatgcc caatcatcgt aactttccgc tttagccaaa gcttgttcgt tgtaatcgac
     5161 atcaacatta gccagcgcat aatcgattgc atcacttgga tattcttcaa actctagttg
     5221 ttcacgcaaa ccttgttcag acataccagc ccaacctaga tagctttcag ctttgctcaa
     5281 tgcatttctg tattcacgag gaacactttc ttcttgagta acttcttcta tagtagtttc
     5341 agtagtttca gtttcttctt caacgactgt agattctgct accgattctg cagaagacac
     5401 ttctgtcacg ctttctttag ttgtttcagc gacggtattt ttagcatcat cactgcctcc
     5461 ttgcgaacct atagcaataa acaccacaac aaccgctaac aaccaaaacc atacccgttt
     5521 ataaaacggt ttcttaactt tatacatttt cccatcttga cccataactt tttttgccat
     5581 ttaaatatcc ctcgcttctt gttataatat atttgtgatc tcagaaatga ggtatgagtc
     5641 cgtgttgcag cacgggcttt tttctttata acttttttag agattatagg caaaatagta
     5701 gggcataaaa atgtattatt gaattccgta tttagaaaat cctaattgaa cttcaccgga
     5761 agtcttttgc tgtgttgtac gcaatgcttc ttcttcagac attccattct gtactttcca
     5821 tgcaacaggc gacatcccgt atttgttaac aaaatcagta agtgataaag tgtcagcgtc
     5881 ttgctgagcg cttgtttgtt ggtcttctgg attctgttga gatgctgctt gttgttcttt
     5941 ctgatcttga ctgataatat tgccagcatc atctgtagtc aatccatttt cataaagggc
     6001 cacgccgaaa gcttcccact ctttgttgga ccaatttgca cgatcagctg gagttgactg
     6061 taaagtgcgt tgtttcatct gttcatatgt ttcttcttga ggtgcggttt ggattgtact
     6121 ctgactggag cttataactg ttgggctagg ttccgccgta gcttggttgg agcttgtaac
     6181 tgttgaacta gaatctgttt tagatgtaga tttgctagta gatgaactgg tttcagttgt
     6241 ttcttttgtt ttacttattt ttgtttcttg gttagaagtg gtatctgttg attcagcttt
     6301 tttattattt gaacaagctg aaagtagcag agcagtactt aacaacaaca taacgctaac
     6361 ttttttcatt ttataattcc tctttctcgt tgtaatatgt gtgctaacac gggctttttt
     6421 atataagaaa acgataagcg ctttctggaa gcccgtaaag attctttaat tcctcgattt
     6481 ttttaggata ttgatcatta tcttctttat aaagagaaac aatgagatta gcagcaaagc
     6541 aattagcttc gctttcagat ttgcttctag atgttcttgt tgatacataa tagctggata
     6601 agccacgatg aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat ctttcctcag
     6661 agtctcgcag ttcatcattt aaaaagatta tcgcacgatc tctaatttct tgaaactgcc
     6721 ccttgggatt ctcgataaaa ggaacgtatt gaattttaat gcccatcttt tcacaaatat
     6781 aaaaaggatt agcggactgg tatttccgct tcaactcctc gactaaatta atcgtatcca
     6841 tctccataag ctcacatctt tttgcctttt tctttgtctt ctttcacaat atcccagaaa
     6901 gtcgctatca ggatatcttt tacgcgctgt atttgttcgg gtgtcaatgt ttccccacca
     6961 taagacatat taacatttga gtctagtagt ttatcaagtt caaccacttc ctcttttgta
     7021 gcccacttgg gaacattatt atttcccaat aaataatcag ttgtgacacc aaaataatca
     7081 gcaactttct ttaagttctc agattttggc gaggctttat cccatcttct tatttgtcca
     7141 ttagagatgc ccacctgtct ttctacttct gctatagtca catgcttttc gtctgctaat
     7201 tctttaatct tagtaactaa ac
//
LOCUS       DAZKCC010000109         7222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000109 DAZKCC010000000
VERSION     DAZKCC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.112"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(234..911)
                     /gene="phoU"
                     /locus_tag="ACG8VH_002678"
     CDS             complement(234..911)
                     /gene="phoU"
                     /locus_tag="ACG8VH_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9111179.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(924..1682)
                     /gene="pstB"
                     /locus_tag="ACG8VH_002679"
     CDS             complement(924..1682)
                     /gene="pstB"
                     /locus_tag="ACG8VH_002679"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9111180.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1698..2504)
                     /gene="pstB"
                     /locus_tag="ACG8VH_002680"
     CDS             complement(1698..2504)
                     /gene="pstB"
                     /locus_tag="ACG8VH_002680"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9111181.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2519..3403)
                     /gene="pstA"
                     /locus_tag="ACG8VH_002681"
     CDS             complement(2519..3403)
                     /gene="pstA"
                     /locus_tag="ACG8VH_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9111182.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3403..4323)
                     /gene="pstC"
                     /locus_tag="ACG8VH_002682"
     CDS             complement(3403..4323)
                     /gene="pstC"
                     /locus_tag="ACG8VH_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP9111183.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4438..5295)
                     /locus_tag="ACG8VH_002683"
     CDS             complement(4438..5295)
                     /locus_tag="ACG8VH_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9111184.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5632..7080)
                     /gene="cls"
                     /locus_tag="ACG8VH_002684"
     CDS             complement(5632..7080)
                     /gene="cls"
                     /locus_tag="ACG8VH_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9111185.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2285 t
ORIGIN      
        1 tttttttgca ttctttatcc tttctatgaa atgatgaaaa gggtttgtga catgttttgt
       61 cacaaaccct ttcccgaata aacggtgttc aaaagcttgc atctgcggta ataagtccag
      121 ccaaacgaaa aatatgagga gctatttttt gtttgactgt tcttattact tgatgtagaa
      181 cagctttttc acaacctctt ttcataaaca aacggcataa tcacagcttc agtttagaaa
      241 atctcattgt ggttcgtatt taactcagtg attttccctg tagccaagta gacgatccat
      301 tcacagatgt ttgtcacata gtctccgatc cgttctagat aactggcaac attcaagtag
      361 tctgttccac tgatgactgt ctcaggattt tcctgcatat ttttgattgt tgcattatag
      421 attcgattga aatagtcatt gactctttga tccatattgg cgatcgtacg tgcgtctttt
      481 tcgtctgttt tgacataagc aactaggacg ttgtccacca tttttttcac ataatcggac
      541 atatcggaga tctctttttc gatttccggt attctcgtct gcccttttac gcgaatcgtg
      601 gatttagcaa cagacaccgc atgatcggcc atacgttcta aatccgagct agctttcatg
      661 accgtaatga tcatacgcaa atcagttgta actggctgtt gcaatgcgat catttcaaaa
      721 cttttcttct ccaagcgtgt ttccatgtta ttgatattta tatcgttttc gataacttct
      781 tgggctaaga ttttatcgtg tttgatgtat gcacgaactg acttgtgcac ggcgctgctc
      841 accatcatcc ccatttcgta gaactgatta tggagattca gcaattcttc ttcaaattgt
      901 gtacgtagca ttccgtttcc tccttatcca aatcgacctg aaatataatc ttctgtctct
      961 tgtttttctg gattcatgaa tatcttcttc gtatcgttgt attcgatcaa gtgaccatct
     1021 aaaaagaatg ccgtcttatc tgaaatacgt gaagcttgag acatattatg ggtcacgatg
     1081 atcatcgtat accgctctct taattctaac agcatacttt cgattttccc agtagaaaca
     1141 ggatcaagcg cacttgttgg ttcatccaat aagatgattt ccggattaac tgccaaaaca
     1201 cgtgcgatac atacacgctg ctgctgcccg ccggataaag ataaagcact tttatgcaat
     1261 ttgtctttca catcatccca aacagacgct gccttcaggc tttcttcaac tacttgatca
     1321 aggacttttt tatctttttc cccttttaat ttcaatccat agatgacatt ttcatagata
     1381 gaaaaaggaa atgggtttgg ctgctggaat accatgccga tttcttttct tagttcaaca
     1441 acatctgttt ttggaccata gatgtctttg tctttatata aaacacttcc ggtgatcgtt
     1501 acattcggaa tcaggtcatt catccggttc aaagaacgca gataagtgga ttttccgcaa
     1561 ccagaaggac cgatcatcgc agtgatttcc ccttgattga aacttagatc gatccctttt
     1621 agtgcttctt tctttccata atacagatga agatctttag atgaaataat ttcttttgtc
     1681 atgttgaggc ctccgtctta accaaaatga ccggacacat aatcttcggt tgcctgaatt
     1741 tttggtctgg taaatatctt gttagttttg tcatattcca ttacttttcc tagatagaaa
     1801 aaggcagtat agtcgctgat acgagctgct tgttgcatgt tatgcgtcac gatgatgatc
     1861 gtatagtctt ctttcaagtt tacaagtgtt tcttcaaccg ttcctgtcga aatagggtcc
     1921 aaagcacttg ccggctcgtc tagcagcaag atatcaggtt tcatcgcgat cgcacgagca
     1981 atgcataaac gttgctgttg cccgccagat aaagccaagg cacttttgtc caagctgtct
     2041 tttacttggt cccacaaggc agcttgtttc aagcttgttt caacgatttc atctaatttc
     2101 tttttgtttt tttcgccgtg ctgtttcaaa gcaaatgtaa tattgtcata aatcgacttg
     2161 ctgaacggat taggacgttg aaaaaccatg ccaatccgtt tgcgcatctc ataaacatcc
     2221 acttgaggcg cattaatatc tacgccttga tacatgattt tcccagtgat tttcgtatta
     2281 gcgatcccat cattcattcg attcaacgaa cgaagatacg tagattttcc gcagccggaa
     2341 ggaccaatca gtgcagtgat cttatttttt tcaaattcca gatccactcc tttgattgct
     2401 tcattgtcgc cataccagac atgtatatca tccgtatata gtgctacttc tttctctttt
     2461 ggcatggtga tgacatttgt ttcattccag ttatattttt tcattttgct tcttctcctt
     2521 aggctgaagt catttttcgg tataatcgtt tgccaatcgc acgagcgccg aagttgaaca
     2581 gtaaaacagc aatgatcaac acggcagaag cgccagccga aacagaaaca ccatcaggca
     2641 tcgtgccttc tgtattgatc ttccaaatat ggacagctaa tgtttcagct tgacggaaaa
     2701 tgctgatcgg gcttgaaaca cttaatggat tccagttcgt aaaatctaac gcaggtgcac
     2761 tttgtccagc cgtatagatc aatgcagctg cttcaccaaa aattcttcca gaacttagga
     2821 tcacaccagt caaaatccct ggtgtcgctt ctggaaccac cactttcatc actgtttccc
     2881 agcgtgacaa acctaaggcc aatcctgctt ctcgttgtgt gtaatggacc gcttgtaaag
     2941 actcctcaac tgttctagtc aaaagaggca agttgaatac agtcaaagcc aatgcaccag
     3001 aaataatcga gaaaccatac tggaattgaa taacgaaaac caagaaaccg aataaaccga
     3061 caacgacaga aggcaacgag ctcaagattt caatcgacgt gcgaatcaca tctgttagcc
     3121 agttttttcc agcatattct gaaagataga tacccgcacc taaagaaata ggaaaactga
     3181 tgatcatcgt gatcaaaagc aaataaatcg agttaaatag ctggatcccg attccaccac
     3241 cttcttggta ggctttggaa ggttcggtca agaaggacca tgaaatgtgc ggtattcccc
     3301 gaactaagat ataaagtagt aaagctgcta aaatcaatac gatgatcccc gaaactatat
     3361 ataaaacacc tgtagcaatt ttatctgagc gttttgcgtt cattatttca gctctccttt
     3421 cttcccaatc atacggatca cgatattaaa gaataatgac atcaatagca agatcagcgc
     3481 taatgaccat aacacattat tttcaactgt tcccatgatt gtatttccga ttcccattgt
     3541 taagatggac gtcaatgtag aagcaggagt agtaagtcct gttggcatca atgctgcatt
     3601 accgatgacc atttggatag ctaaggcttc accaaacgca cgagccatac caaaaacgac
     3661 tgctgttaaa atcccgggca cggctgctcg tagcacgact ttgtagattg tttgccatct
     3721 tgtggcacca agcgctaatg aagcttcccg atagtgccgt ggtactgcct ttaatgcatc
     3781 aacagtcatc gttgtgacag tcggcaagat cataacgaac aacacaaatg taccagctaa
     3841 gataccaaac cctgtcccgc caaaaatact tctgacaaaa ggcacgataa ctgataaacc
     3901 gataaaacca taaacgacag aaggaatacc tacaagcagc tcgatgaccg gctgtaagat
     3961 cttcgttccg tttttaggag agatctccgt catgaatact gctgcaccaa tggcaaatgg
     4021 cgtggcaacg atagcagata aaaaggtcac aataaacgaa cctgtgatca taggcaatgc
     4081 ccctaccatt ggtttcccat ttgctccgat ttggcttgga ttccaatctg taccaaacag
     4141 aaaatcaaat acattgatct tatcagtaaa aaaggtggcc aatcctttac tagctacaaa
     4201 ataaaaaata gataatacaa ccaacacgat cagcgcgata cataaaaaac tgatgaattt
     4261 tccacgctgt tccatttttg aacgttttga ttttgttaat aatacttttt tcacatcttc
     4321 cacagatgac tcccccttaa tcaggtttaa catactcact cagtgtacaa attgaacatc
     4381 aatttggttt taataacatg taaaagttta gctaagtttg taaattttgt gtaaagatta
     4441 ctctttaacg atatttcctt gccaatcacg ttcgactttc atatgagaaa cagggatata
     4501 acctagttct ccaacgatgt tatcttgtac ttcatcagat aagatatagt ctaaaaattc
     4561 ttttgtcagt ttttttggat gatctcttgt gtacatatgc tcatatgccc aaatcgtcca
     4621 tttgttcgtt gtcacatttt catctgtcgg ttctacaccg ttaacattca acgtatctac
     4681 atcatcggaa acataagaaa aagcaacata gctgattgca ccgggtgtgt cggcaacgat
     4741 ctggcgcacc attccgcttg aatcttgttc ttgtgcctga acagctgttt ctccgtccaa
     4801 tacccatttt tcaaacgtgc tacgggttcc gcttcctgaa gcgcggttca atataactac
     4861 tggctgatct tttccaccga cttcactcca gtttttgatc tcgcctgtaa atagtttttt
     4921 caggttttcc atactgatgt cttttacgcc aatatccttg ttcacaatag gagtgatccc
     4981 aacaactgct actcgatgat cgactaatgc tgaggcatcg atccccgatt tttcttccgc
     5041 aaacaaatca gagtttccga tgtcgactgc tcctgactgt acttgagaca aaccagttcc
     5101 gcttccgccc ccttgtacat tgatgaattt tccaggattt tctgtctggt aagtttcagc
     5161 aactgtttct actaaaggct gtaaagcaga agagccaaca gccgtaatgg attctcctcg
     5221 atcaatccac tttgaacagc ctgataaaag aatggctaat cctattattg gtaagaataa
     5281 tttaagtttt ttcattagaa atctccttta atcctaaagt atgtttattt tatcattttc
     5341 attacttgat gaaaacgaat caaaatcatt aaataacaaa ataaattctc ataattgtaa
     5401 aaaaaactat ctcattaaaa gtttctatgc tatactggct ttgaaatgtg tctcttttaa
     5461 tagcatatat tagcttattt cttgctttcc ccaaaaaaca agagataccc caaagttaat
     5521 ttcgctcatg cgagacgctt tctccatttc tgacgtactc tcagaaatgg ccccgttaga
     5581 aaaggacacg agatctccat ctgttgatgg ttctcatgtc ctttttatgt tttaaaggag
     5641 cggcgaaatc aatcttgcta atccttcttt catcttgatg attattcctc ggttttcata
     5701 catttctttt gtgagatgcg tagaaatttt cgagtcttca aaaaaagctt ttcttacacg
     5761 tgaagccatt cgttcatcat aaaccagtgt attgacctca aagtccaagc gaaatgaacg
     5821 aacatcgata tttgctgagc caactgaaac aatgccacca tcgataatca tcgtctttgc
     5881 atgaataaat ccattctcat atgtttcgat ccttgctcca tactcgatca gctctgcagc
     5941 gaaagaatac gttgcccagt aaacgagaag atgatctggt ttgttaggta tttggatatg
     6001 aacttttacg cctgaaagaa gagccaactt cagtgcttca tgaatcgatc catccggtat
     6061 gtaataaggc gtttggatca atatctcccg tttggctaac gaaatcattt tcaaataagt
     6121 cattttgatt tgttcatgtt cggtatctgg tccgcttgtc acgatttgaa cagctatatc
     6181 accagtagtg tgaatggacg ggaaaaattt gggagaatag cctacttctt ttgtatgctg
     6241 agaattccag tccatcaaaa aacggttttg caaggtatac accgcttctc cgtaaatacg
     6301 caaatgattg tctcgccaat aaccaaattt ctttaccaat cctagatatt catttccaac
     6361 attgaaccca cctgtatatc cgattttccc atcaataact acgattttac gatgattgcg
     6421 ataattgatc cgaggattaa tatacggaac aaatagagga aagaaaaatg cgacttctcc
     6481 gcctgctttt ttcaaattct gaaaaaagct ggaagaaact tgtgtggaac cccatgcatc
     6541 aagaaggact ctgactttta cccctcgtct tgccgcctct gtcaatgcat ctcgaacttc
     6601 ttcacctaat gcatcactgc gataaatata atactggaag tggatatggt cttctgcctg
     6661 cttgatatct tcaatcagcg catcgaattt ttcacgacca tctgtaaata aagtgatttc
     6721 atttttagtc gtatagagcg tactttcaaa taccgtgagc atatagatca attgtttcac
     6781 atctacttgt ccagtaggcg gatgaggata aagccctcgc atcaatgcct gtttgtcttc
     6841 ttcaatttcc aggttcttac cgattttccc ttggatcttc aaatcaaaga ttttctcttt
     6901 ggaaatccct cttcctaaaa aaatatataa aataaatcct acaactggaa tgaacatcaa
     6961 aacgagcagc catgcccatg tttgagcagt ttcctttctt tctcgaaaaa cgatgataaa
     7021 ggaaagcaaa atattcataa tcaaaaggat cgtaaaaaaa ttgtcaacga taattttcac
     7081 ggcttcacct cttcttctac caacttaaga atacccaagt tgttttttaa agtaaaggga
     7141 aaaaagccgc ttccttctat tctcctagcc aataaaaaaa gaaagctgga acgaaactgt
     7201 ccaactttct cattttattt at
//
LOCUS       DAZKCC010000110         6862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000110 DAZKCC010000000
VERSION     DAZKCC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.113"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(513..1724)
                     /locus_tag="ACG8VH_002685"
     CDS             complement(513..1724)
                     /locus_tag="ACG8VH_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9111186.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1842..2477)
                     /locus_tag="ACG8VH_002686"
     CDS             complement(1842..2477)
                     /locus_tag="ACG8VH_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP9111187.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2560..2982)
                     /locus_tag="ACG8VH_002687"
     CDS             complement(2560..2982)
                     /locus_tag="ACG8VH_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9111188.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(3000..3320)
                     /locus_tag="ACG8VH_002688"
     CDS             complement(3000..3320)
                     /locus_tag="ACG8VH_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111189.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3613..3804
                     /locus_tag="ACG8VH_002689"
     CDS             3613..3804
                     /locus_tag="ACG8VH_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111190.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            3818..4069
                     /locus_tag="ACG8VH_002690"
     CDS             3818..4069
                     /locus_tag="ACG8VH_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFP9111191.1"
                     /translation="MEQLALVNLSALKASLTESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            4045..4224
                     /locus_tag="ACG8VH_002691"
     CDS             4045..4224
                     /locus_tag="ACG8VH_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111192.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(4267..4737)
                     /locus_tag="ACG8VH_002692"
     CDS             complement(4267..4737)
                     /locus_tag="ACG8VH_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111193.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            4824..5522
                     /locus_tag="ACG8VH_002693"
     CDS             4824..5522
                     /locus_tag="ACG8VH_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9111194.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            5700..6041
                     /locus_tag="ACG8VH_002694"
     CDS             5700..6041
                     /locus_tag="ACG8VH_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111195.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            6034..6705
                     /locus_tag="ACG8VH_002695"
     CDS             6034..6705
                     /locus_tag="ACG8VH_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9111196.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
BASE COUNT         2233 a         1114 c         1215 g         2300 t
ORIGIN      
        1 ttgtattaat ggaaaagggg aaagcagcaa gtaaatttag tttttctatt aaatattctt
       61 cactttttaa ccttttatag tacttagatt taatcctata tgtttagaga gttcattggc
      121 agcatgattt tgcaaagctt ctcgaattat tgttatatta gaaactttac tgttcataat
      181 tatgcttctt ttttaactca aagtccaaag ggctacagtg gactttgagt taaaaataaa
      241 aagccctagt tattttgaaa aataaaaaaa gaacagcgta aatgttttcg aaatgctttc
      301 gcaccaacat ttacctgttc cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc
      361 cgcgacccgc tgattaagag ttcgttattt aatggtattt taaatgaaga taagcgaatt
      421 tacagcattt ttaaatgctt atataccgca aattatccca agtttttata tatcctaccc
      481 gagtttatac ccgaaaatat aattttttag tgttatttta tatctttttt taatagtttt
      541 tcaaacgctg tagttgctgt atctttttct ttgcgtaaga catggccata aacattcatt
      601 gtagtattaa aatctcggtg tcctaatcgt ttttgaagct cttttggagt catattagga
      661 tcgctaagca aataggtagc tgatgtatgt ctaatgtcgt gaaaacgaat gtgacgcaaa
      721 ttgtgacgtt tggtgaatcg tttgaacttc ttatacagat aacttccacg tggtaaagtt
      781 ccgtcttgtt ttgtatcaaa tataaaatag tgtcttaatt tctttatatt taatttccaa
      841 cgaatttttt tattctcttt gatcaattca tctaacatat caaatagata agcaggaccg
      901 gttactattt tttcatcatc atcatttttc aaacctggca gcaaccgaat atctgatttt
      961 ccatccactt ctgaaatccg ctgatgaaat cttatttctt gtttgtcaaa gtcaatatct
     1021 ttttcttcta aagctgcaat ttctccttgt cttgctcctg taataaatgc tagaacaatt
     1081 aaagttttaa tttctatgct ttcctcaaat gctgctttta gcattgcatg aatttcttct
     1141 tccgaataag gttcttcgat attctttttt acgccttttt cttttggtat ttttacattt
     1201 tccacaggat tttctttaat tgctccgtac tcatttttag ccatcaaaaa taagttgtta
     1261 acggcgtata acatacgaag ctttgtattt cttgataaag gtttatcaga gtttcttcca
     1321 ggatcttttg tattgattct ttgagcatta acaacaattt tctttatcat gtatggtttt
     1381 atatcgatta gagggataaa atcaaattta tcaagaaatc tattctcgat cattcgctta
     1441 tagttatgat aagactttgg ctctaaattt ttttcggcct ctgaaagcca tatttttcta
     1501 taaaattcac caaaagttat attgaataaa tccaacgaat catcggtcat tccttcgaat
     1561 tgctctaacc agatgttcaa ttctttatat gccgctcttt tactattcgc ttttatagtt
     1621 ttgctttttc tttttgccga gccatcaggt ttgtacccta atacagctct tagtctatat
     1681 gtttcattgt ctacttgttc taagtatcct gttacttctg acataattga atcagctctc
     1741 tttctctgat acaataggta ctataaagaa gcctattgtg taggtttgtt ttttcttaga
     1801 acacgctcgc tttggtcggt ggggcgtgtt ttttatacta attagttatg aagagcaatt
     1861 gaaactgtct ctgttccaaa gtaacctgtt tgtgcttgca atacattatt ttgtgcattt
     1921 gcttgggctt cagagatatc aaaaactatt ttaccagttt gttgcatatc aggatttaag
     1981 ctttccataa agaaagagtt agtgatatta ccattttcat cttgatttgc cgacatagat
     2041 gcagccgaat cagcttcgaa agtttttcca tctgctttta atttgaagaa tgagctatca
     2101 acagtcacag ccttatcacc tgcatttttt acagataagt caactactaa gaaagtgtct
     2161 tttgcgttag taggaagtac agatgggcca acttgtttta ctacttcaac ggagtttact
     2221 ttgtattcca ttttccctac agcaacgtca tcaccgattt tataagtctt ttcttctttt
     2281 gttgtagttt cttttgtagt ttctttatta gtacttgttg atgttgcagt agctgaatct
     2341 ttgttttcac cgccacttag agcaccacca ataccaaaaa acaaaagtac tattaatacc
     2401 caaaaccata cccgtttata aaacggtttt ttaaccttat acattttccc atcttgaccc
     2461 ataacttttt ttgccattta aatatccctc acttcttgtt ataatatatt tgtgatctca
     2521 gaaatgaggt atgagtccgt gttgcagcac gggctttttt tactgtgcat aagagtattt
     2581 tttcttgaaa tatgactggc aaacattaaa acattctgtt cttaacttat tattgataga
     2641 gtagaattcc atgaaatttt ctaatttgaa ctgagattca tcagtcaatt cgttctcaat
     2701 aaagatattt agtaaaatca taattgcgat tctatcagct tcagcttcga attttgaatg
     2761 aaaagttgta gagttatcgt acagtactga atattcaaaa tgtgaagcaa tgaaatgacc
     2821 gagctcatgg gctaaatgaa aagcttcaga actgtcttcg tgtagttttt cattcaaaaa
     2881 tactattctt ggttttggat aataaaaacc tggttcttcc atttccatat agattaactt
     2941 taaattatac tcactcagca tttctttcaa ctttaaatac atacaaccca tcactccaac
     3001 tattcatttt cctctaaagc tttagcaatt gcaatcgctt tacgcattgt ctccttagat
     3061 atttcttttc catcaaaaga aaatacagta tcatcttctg ataaatccac atgtttaggc
     3121 gtttcttttt cttctctacc tagaagatag tctacagaga cattgaaata gtcagctacc
     3181 ttttgcaaat ctcttgcttt gggttctgtt gttttccatc gatagaaaag attttcgcta
     3241 aatcctaatt ctaaagcgac ttctttcatg cttttatctc tttggctagc taatgatttt
     3301 atcctttcaa acagtgtcat aataatgttc tccaatccta aacaaataaa agtacacaaa
     3361 atgtaaatta tactgttgac ataagtataa gtttgtatta ttatttgttt gtaagttaat
     3421 ttgatagaaa aaaagcaaac taaaaataca ccttatagca ttaagtttgg cgaccgattg
     3481 cataataaag ggtttgttgt aggcttattt aactatgttt atatagtata cgattgtata
     3541 ctattagtca atagtttata cgttttttct atcattttga cttacaaatc tatatgtaag
     3601 gggagtgaat agatgtcaaa tctagataac ggccgtgctg caatcaagaa gtttatgcga
     3661 gaaaacggag taactatgga agatttggct actgcatatg gatacacacg tgttcgaatg
     3721 cagcaaatta ttgatggcca ttggagtgga ccaaaagcta ataaaactat cttagaaatc
     3781 atccgtgatt acagaattcg ataggaggaa ttaatcaatg gaacaactag ctttagttaa
     3841 cctctcagcc ctcaaagctt ctttgacaga atctgagatt gcaaatgaag tgtgggatac
     3901 aaagcaagct gcggaatatt tgaaaaccac gacacgcacg ctaactaaag atgccgaatc
     3961 tggaaagatt ccagcagcga aagttggcag agagtggagg tttagtagta tcgctttgta
     4021 tcagtatcta aaaggaggaa aaacatgggc aaattcaaca gagcactagt attcagcgca
     4081 ccgctaatca tctacgcttt agggctttgg ggaaatagac aagcgttgat aggaacgatt
     4141 gtttacatgg tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa
     4201 aagccaaccg agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac
     4261 tattactcag tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc
     4321 aacaattttg tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag
     4381 gagtttatta aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt
     4441 aggaggaata tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc
     4501 tttagcaatt aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga
     4561 aagttctaaa ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt
     4621 ataaatcata tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat
     4681 tccttttatt tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca
     4741 ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta
     4801 taccaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg
     4861 tgactacgag cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag
     4921 atattgatgc actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg
     4981 aaccagattc atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct
     5041 tgctggctat ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag
     5101 cattcaacga aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata
     5161 tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag
     5221 atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag
     5281 ttatgggagt tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat
     5341 tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac
     5401 ctcgtaagag attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga
     5461 tccgtatgga aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat
     5521 agagctgaag cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa
     5581 ccaatcatcc taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag
     5641 tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata
     5701 tggaaaaaga actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga
     5761 aaattgagga aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag
     5821 cttgggatca agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc
     5881 atctatcgaa gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg
     5941 atgcgctaga agagtacgac agagataact tgattgatat ctcttattac ggaccaggta
     6001 agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac
     6061 tttgaaaaac tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat
     6121 tatttatctt gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta
     6181 actattaatg agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt
     6241 gaggaatata aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg
     6301 gttaaaggac acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt
     6361 gttaaaccaa atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg
     6421 gctctacacg gactgggatt atatgtattt caaggggaag atattccaac accacctaga
     6481 atcgatacaa agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg
     6541 ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg
     6601 ctcttagctg ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg
     6661 gagcgagcaa tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac
     6721 cattaatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa
     6781 ccatcaatga ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg
     6841 atcgagtcat tgaaattcgt tt
//
LOCUS       DAZKCC010000111         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000111 DAZKCC010000000
VERSION     DAZKCC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.114"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8VH_002696"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8VH_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8VH_002697"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8VH_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP9111197.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8VH_002698"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8VH_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8VH_002699"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8VH_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP9111198.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8VH_002700"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8VH_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9111199.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8VH_002701"
     CDS             3582..4271
                     /locus_tag="ACG8VH_002701"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9111200.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8VH_002702"
     CDS             4285..5787
                     /locus_tag="ACG8VH_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9111201.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8VH_002703"
     CDS             5800..6276
                     /locus_tag="ACG8VH_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9111202.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8VH_002704"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8VH_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKCC010000112         6580 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000112 DAZKCC010000000
VERSION     DAZKCC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.115"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8VH_002705"
     CDS             complement(99..1229)
                     /locus_tag="ACG8VH_002705"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9111203.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8VH_002706"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8VH_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9111204.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8VH_002707"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8VH_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9111205.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8VH_002708"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8VH_002708"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9111206.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8VH_002709"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8VH_002709"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111207.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8VH_002710"
     CDS             4880..5692
                     /locus_tag="ACG8VH_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111208.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8VH_002711"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8VH_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111209.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1996 a         1330 c         1029 g         2225 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct
//
LOCUS       DAZKCC010000113         6533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000113 DAZKCC010000000
VERSION     DAZKCC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.116"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..879
                     /locus_tag="ACG8VH_002712"
     CDS             <1..879
                     /locus_tag="ACG8VH_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9111210.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPL
                     EVFLSYVSIDDLSNLI"
     gene            997..1149
                     /locus_tag="ACG8VH_002713"
     CDS             997..1149
                     /locus_tag="ACG8VH_002713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111211.1"
                     /translation="MFSGDWGIVSYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLK
                     EKKTSN"
     gene            1356..1556
                     /locus_tag="ACG8VH_002714"
                     /pseudo
     CDS             1356..1556
                     /locus_tag="ACG8VH_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2268..2873)
                     /locus_tag="ACG8VH_002715"
     CDS             complement(2268..2873)
                     /locus_tag="ACG8VH_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111212.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            3055..3390
                     /locus_tag="ACG8VH_002716"
                     /pseudo
     CDS             3055..3390
                     /locus_tag="ACG8VH_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3555..3770
                     /locus_tag="ACG8VH_002717"
                     /pseudo
     CDS             3555..3770
                     /locus_tag="ACG8VH_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3908..4879
                     /locus_tag="ACG8VH_002718"
     CDS             3908..4879
                     /locus_tag="ACG8VH_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9111213.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5019..6332
                     /locus_tag="ACG8VH_002719"
     CDS             5019..6332
                     /locus_tag="ACG8VH_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFP9111214.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            6375..>6533
                     /locus_tag="ACG8VH_002720"
     CDS             6375..>6533
                     /locus_tag="ACG8VH_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9111215.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENF"
BASE COUNT         2164 a         1062 c         1293 g         2014 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata atattcctag aaaatcactg gattatcgaa cacctttgga agtatttttg
      841 agttacgtaa gtattgatga tctgtctaac ttaatttgac aattaagaca tttaaaaaga
      901 aaagtgacag ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt
      961 ctaatactag gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt
     1021 gtcagctatt ctatattaac attgattgct atggttttag gtacttcatt ttataatgct
     1081 tttgtaagta agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca
     1141 agtaattaat atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca
     1201 cattaagaac gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc
     1261 atatttgttt gttatgctat acatagatct gcataattag acttgcgctg cccgttattg
     1321 aagtttcagg cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga
     1381 aacgctggca gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct
     1441 accccactgt tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag
     1501 atatagatac ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag
     1561 aaacaagcga agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca
     1621 actctaagct cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa
     1681 tacatggatg tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag
     1741 ttgagtattg cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag
     1801 atagctccat ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc
     1861 tgtataatag aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg
     1921 ttcactatcg aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt
     1981 taactgtggg aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg
     2041 gggtgactga actaaccgct gatatgagcg atacttggct gtaggaatat ctttttccgg
     2101 aaaaacaaac aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac
     2161 taatgaaaaa gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat
     2221 agaaacgaaa atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc
     2281 gctttcttta ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca
     2341 actggaactc cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga
     2401 tctgttgagt tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc
     2461 aataatactc cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa
     2521 cttagcaatg ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta
     2581 aaatctaaaa actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta
     2641 actgtatttt ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca
     2701 caatcacctt ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta
     2761 ccactggctt tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt
     2821 cgatctaaat tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact
     2881 ccttttaaaa aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata
     2941 gcatttttaa aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat
     3001 gaaattaagc gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag
     3061 ttaaataaag gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc
     3121 aaactttatc aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta
     3181 actaatgcta tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa
     3241 acggaagggt tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat
     3301 cacattaatt ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata
     3361 ctacgtccgt taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca
     3421 ttttcgcacg gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata
     3481 ctaataaatc aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat
     3541 gttccatgta ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact
     3601 tagtcgtggt gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt
     3661 aggctgctgg aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat
     3721 tgacaaagta tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa
     3781 atttttttac gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa
     3841 tttggaatcg tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt
     3901 tcgtgaagtg aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac
     3961 ggtatctaga gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc
     4021 tgcaattagt gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag
     4081 tagcaataca atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc
     4141 ctactatgta gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa
     4201 cggcagtgcg gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg
     4261 aattataggt attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt
     4321 cagtatagac cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg
     4381 tgggcaaatt gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg
     4441 gggtgtttca ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg
     4501 tgaggaaaat aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga
     4561 ggaacaattg ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa
     4621 tgatttcatg gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa
     4681 ggattttcaa attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact
     4741 atcaacgatt gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc
     4801 gatgatttta aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg
     4861 agagacgact aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga
     4921 aagtgatttc aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa
     4981 ataaaacgga aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa
     5041 ttgcagcaat tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa
     5101 gttcaggtag cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc
     5161 ctgaaggaaa agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat
     5221 ataaagctgt tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag
     5281 taaatgctgc aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata
     5341 ctgcagctta tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg
     5401 acatggatga tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg
     5461 cgtttggttt ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg
     5521 ctggaattac agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc
     5581 aagcaatttg ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg
     5641 cgtcgggaga cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg
     5701 atgtcgttaa tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg
     5761 aagcgttgga atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg
     5821 aaaaaggatt tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag
     5881 atttagagga aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg
     5941 caactgggga gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag
     6001 agaaaaagga tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta
     6061 ttatgagttt agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag
     6121 atgaagtgtc ggaaggaatg caggtattaa ttaagcaaaa ccaactttct ggacatgccc
     6181 gcccagtggt tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata
     6241 ttagctactt cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa
     6301 tgcaacaggc aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt
     6361 taaaaaaacc gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc
     6421 tgctgctgac gatttttctg atcatacccg taatcatggt aatttattat gcctttactg
     6481 attattattt gttaacgcca gatgctagac aatttatcgg cttagaaaat ttt
//
LOCUS       DAZKCC010000114         6340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000114 DAZKCC010000000
VERSION     DAZKCC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.117"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..406)
                     /locus_tag="ACG8VH_002721"
     CDS             complement(152..406)
                     /locus_tag="ACG8VH_002721"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111216.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(810..1592)
                     /locus_tag="ACG8VH_002722"
     CDS             complement(810..1592)
                     /locus_tag="ACG8VH_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111217.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1657..2493)
                     /locus_tag="ACG8VH_002723"
     CDS             complement(1657..2493)
                     /locus_tag="ACG8VH_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9111218.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2511..3920)
                     /locus_tag="ACG8VH_002724"
     CDS             complement(2511..3920)
                     /locus_tag="ACG8VH_002724"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9111219.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3934..5757)
                     /locus_tag="ACG8VH_002725"
     CDS             complement(3934..5757)
                     /locus_tag="ACG8VH_002725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9111220.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1947 a         1360 c         1020 g         2013 t
ORIGIN      
        1 ttctttttca acttattatt tctgatttct gtagctgtca acggtttatt cttataaaaa
       61 taatagacac ttctagggat ttctaataac cgacataaca aagaaacagt gtaatcctta
      121 caccattttt caataaattg aacaagcgaa tttaggtttt ccgagagaat atggctgcgg
      181 ctttttttaa gatttcaagt tcctcattta aacgagcatt ttcttttctc agttcagcta
      241 cttcttttcc agtcagtcct gtggactgat caggtaaata taaattcttc catttgtaaa
      301 tcgttgcttc agataagcca tattctttcg ctaaccctcg aacagaacgt ccagtttgat
      361 tcagttcaac aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc
      421 tcgtagataa gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa
      481 caccaagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
      541 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
      601 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
      661 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
      721 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
      781 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
      841 taatcagtta cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt
      901 aaccgaagtg gaatgtcttt accaacttgc tctgtcactg cattactgat acctgttaga
      961 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
     1021 gtgattcctt tatcttcatt tacaagcttc caagtaggtg gtatagctgt gtatgtttcg
     1081 atgaatgaat caattctttc atctatcgtc ttcccagtat ctacttctgt ctcattactc
     1141 ttagatgaat cgattctttc atccatcgtc tgctccgtaa ctgcttctgt ctcattattc
     1201 tcaccggaaa ctctcttcac acccaaactt atatattgtc catctttagt gtgcatagtt
     1261 aaaattccca cgcttttact aataaattca ctataaccag ttctttcaat agagacttca
     1321 atgtatttca cactatcata ctcaaaagaa aaatagacat gtaaaagcat tttgttgttt
     1381 acatctacat aataatcctt tgactctact tgagaccttc ctatacggaa tacagcttta
     1441 ctgtctgata tctctatacc agcatcctta ttattaatct gccaatctcc aagtagctct
     1501 tctggcaagc tagtagtttc ttgtgaacac tttaccactt cactggtaga ggtcgaaaca
     1561 ttcacctccg cactgttaga agaattttcc aaggaatcag gagccttagc acaaccaaca
     1621 agtctttata tattgtgcag ccgatatttt cttcattcag tacacccttg aaatatgcag
     1681 tgttagataa agcagttcgt cctgatttgc tgaggcaccc atctcttctt gaacgtactc
     1741 cttgattttc aaggccgccc gatactcttt cggaaaacgc tgcttcactg aagccagcaa
     1801 aaaatcatcc ttctccggca actcttcctt cttaatcatt cgggcaataa aaaactgtaa
     1861 atgcgtcaca aatcttaagt aaagcatcga atctttatca accttttttt cgggaaacag
     1921 atatcctagc atagccaaga tatcctttag tgctcttacc gagagcatgg tttcttccat
     1981 atttgcttgt tcactttggg catttacaat gtgaaacgca atgtttccag cctcctcttc
     2041 tggcaactga acattcattt ttttgttgat caattggata gcatagcagc cgatctcaaa
     2101 ttcttttggg taatactttt gaatttcaat cagcaggcga ttttgaaaaa caatgccttt
     2161 ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag gtaaaaaata gacttttatt
     2221 cagcttaaag tcaaacttgg cgttggcata atcgataatt cgcttgacgc aaataattag
     2281 atcatccgga gcagcctcaa acagtttaat gtaatccttt tttagagcat cagaaagtga
     2341 atctaattta aatacttttt ggattttgtc agccggaacc aaatcgcctt tctttagttg
     2401 aaatccaatc cctttcccca tcaaaattac ttctttgcct tgcttatcta agcaaagaat
     2461 taaactgttg tttaacgttt taattactct cattagattc accgttctct ctaagctaag
     2521 ccatttcttg cgattacttt ttgataccaa aagaagcttt ctttttttat tcgttttaat
     2581 tctttaagat tctcatcggt tcggtcaaca tatataaaac cataacgctt attgaagcct
     2641 tgatgggtac taacaacatc aatcgctgac caaggtaaat aaccaaaaac atctacacca
     2701 tctgttaaag ctgcttgaat agccagaata tgtttggcta gataatcaat tcgatcctgg
     2761 tcattaattc gtctatcttc ctcaactatg tcgggtgccc catagccatt ttcagtaatt
     2821 aaaataggta aatcatagcg atcgtacaat ttcctcaatg ttaatcgtag tccagtaggg
     2881 tcaactaccc agccatatgg cgttttatct acataaggat tttcagctgc ccggtagaca
     2941 ccttcttctc ccaacatgat ctgttgatcc cccgctctag cggaaacatc tgatccgtca
     3001 tttttgctgg cagcgatggt ggcagttgaa taataattaa tcgcaataaa atcgggttta
     3061 ccggaggata aaattgcctg atcctctgcc tcaattttag gatataatcc acgatcattc
     3121 aaatagtttt caaacaaata attgtatttt ccccgaactg ctacatctaa gaaagaccag
     3181 cctctcaatg tttcccaatt atgggctgca attgcatctt ctggactgga ggtagcttga
     3241 tacatagccg ttaaattgat tgctggaccg atttttcctt taagattcaa ctgatgatac
     3301 aagtgaatta ctctagcttg agctaataaa acatggtgat taatttgaaa gagttctttt
     3361 tttgaaggca gctctccact tggtggcaaa ccaatcgctc caggatgaag gatcatcgtg
     3421 ttttgctcat taattgtcag ccaatagttc accttgtcac catactcagt aagcaatacc
     3481 tgtacatagt ccacaaaata atcgatagtt gctcgattta accaaccgcc ctgctcctct
     3541 agaaaataag gtaaatcgaa atggtacatg gtgacaatcg gttcgattcc ctgattcagg
     3601 cattcatcaa ttacttgatg ataaaaagaa attccctttt gattggtttt attattttca
     3661 atatccataa ttcgcgtcca agcgatggaa aagcgaaaag cttttaagcc taattcttta
     3721 aacagctgta tatcttcttt atagtgatgg taaaaatccg aggcaacttc aaaattagct
     3781 accccttctg tatgctgata cttgtcaata atagacggtt gtttcccatc ctcctgatag
     3841 gcaccctctg cctggtaggc actaatggag gcaccccata aaaacttctc tggaaatgct
     3901 gaaagctgtt tgtattgcac aaaaatcctc tccttaacta atttgcatca atggttcgtg
     3961 ttcctttaag cttccgcttg cttcactaat tcctgcgtaa ttttcactgt tggtcactac
     4021 cacaatcact gtatcatcgt agccagcgtt tctaatggca gcgaaatcgg cgttgattaa
     4081 taattgtcct gctgacacct gatctcctgc ttttacaaat acttcaaagg gttccccttt
     4141 taaattcacg gtgtcgatcc ccacatggat caataattca ataccttctt tagaacgtag
     4201 accaatcgca tgtttagatt ctaaaatagt aatgacttct ccctcaacag gtgaataaat
     4261 gctgttttct tctggatgaa tggctacgcc ctttcccaat agtcccttag aaaagacagc
     4321 atcgttcact tcagaaatag gacacacggt tccttttacc ggattttcaa caatgactgc
     4381 ttcggctatt gccgtttgct ctgattcatc agaagtatcg tcaaatccaa ttagataagc
     4441 aatgataaag gttgcaacaa aggcgattac acacgtaata attgcgtgaa caatattcat
     4501 tggattttct ccaataaagg ctggtaaggc tgctaagcca ggggaaacaa aggcatatct
     4561 gactaaaccg ctcaagccag catacaaacc agcaattgca ccaccaatca tggaagcaac
     4621 taatggtttt ttcagcttca aggttactcc atagagtgaa ggctcagtaa ttcccattaa
     4681 agcagtgatc ccagtggaca cagctagctg cttcaactct ttgtttttag ttttcgcggc
     4741 tacagcaaag gtagcacctg attgagcaat attagaagcc agcattcctg gcccattgat
     4801 catttcagaa cccgtactgc tcaattgcat agtagcgatt ggcgtcattg cccaagctgt
     4861 acccgtaacc accaaaaatg gctgcaaacc acccatcaaa aatggaatta accaagctgc
     4921 tttgccgtta atccattcag cccccattgc tataagatcg ttcagcaacg ttccaaacgg
     4981 tccaatgaca accaacgcta aaggccccat aattaaaaat accagcatcg gcttagtaaa
     5041 gaatttaata aacgaaggcg aaattcgatc cgcaaagggc tctacatagg acatgatcca
     5101 aacagtaaag ataatcggta aaactgatcc cgcataatct gcagctaata ccggaattcc
     5161 tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa atatttggat gaagcaaaat
     5221 gccagcaaca gtaatcgcca aaattgggtt gacatcaaac tttatggctg ccccataggc
     5281 taatagtact ggcataaaat aaaaggctgc atcagcaata gtattcaaaa tgatgtaagt
     5341 ttgactactg tctgatagta caccagtcaa agtcaagacg gccaaaatgg cttttaccat
     5401 cccggcacct gttatagctg gaatcatcgg tgtaaaggtg gtagaaatga cactaatcaa
     5461 tcttgaaata atactttctt gattgtctga agaggggccc tttttaattg aagttcccac
     5521 ttccttttga atttccttga aggctgtttg gacttcattg ccaatcacca cttggtattg
     5581 tccaccgcta tccaccacac taattacacc aagtgttttt tctaaggctt tcgtatcggc
     5641 tttcctttta tctttgagtt caaaacgcag tctggtggca caatgggtta agctgaccac
     5701 attttctttt cccccaacct gttgcaatac atttcttcct acttctctgt aatccattat
     5761 tctctctcct taaagcagta aaaaagacca agtcaaacca cgccccaatc aacattgaag
     5821 tgttagtttg tcttggtctt gcctgcataa tcagtaacat gccaataacg ttattttgct
     5881 gtaatccaat aatagcacat tatgatagcg caatcaacag ggaatttaat tttttatctt
     5941 ttcgtttgac cgtatagggt aatataacag ttagatcaca tcaactaggg tatatctgcg
     6001 tatacttact gctaaaataa gtatatctaa tattaactat accagtaaaa ccattcctac
     6061 ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa aatgcatata acggatcaca
     6121 caacttcaac aattctgctc ttgggaaaaa ttgatgaatt tggtgagtaa aggatggaat
     6181 aaacagcatt ccaacaatat aggcaacaat agtgatactt gtttttttgg taaaaatcag
     6241 taagcaatag ccaatttcaa tcattccgag atataaaggt atcgatagaa tgatttgggc
     6301 tagtattctt aaaaattaac tagcaaatgt ttaaactgat
//
LOCUS       DAZKCC010000115         6243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000115 DAZKCC010000000
VERSION     DAZKCC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.118"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..462)
                     /locus_tag="ACG8VH_002726"
     CDS             complement(136..462)
                     /locus_tag="ACG8VH_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111221.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(508..870)
                     /locus_tag="ACG8VH_002727"
     CDS             complement(508..870)
                     /locus_tag="ACG8VH_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9111222.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(907..1121)
                     /locus_tag="ACG8VH_002728"
                     /pseudo
     CDS             complement(907..1121)
                     /locus_tag="ACG8VH_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1249..1584
                     /locus_tag="ACG8VH_002729"
     CDS             1249..1584
                     /locus_tag="ACG8VH_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111223.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1660..3657)
                     /locus_tag="ACG8VH_002730"
     CDS             complement(1660..3657)
                     /locus_tag="ACG8VH_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111224.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3755..4030)
                     /locus_tag="ACG8VH_002731"
     CDS             complement(3755..4030)
                     /locus_tag="ACG8VH_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111225.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4262..4996
                     /locus_tag="ACG8VH_002732"
     CDS             4262..4996
                     /locus_tag="ACG8VH_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111226.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5035..6177
                     /locus_tag="ACG8VH_002733"
     CDS             5035..6177
                     /locus_tag="ACG8VH_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9111227.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2125 a         1144 c          897 g         2077 t
ORIGIN      
        1 aaaattcccg acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag
       61 gaaactataa aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg
      121 cataatacat ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat
      181 acggtctttc tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat
      241 agctttagcc cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga
      301 tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc
      361 taatccagat gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat
      421 aactataatg ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc
      481 caaaataatt tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa
      541 gattgctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg
      601 atataagtag cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc
      661 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
      721 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc
      781 agttgtctca tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt
      841 tatttctttg ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt
      901 tattagttaa ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact
      961 taatatctca gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1021 cgaacttaca cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct
     1081 ttttcttttc tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt
     1141 attatataca acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca
     1201 ttacaaatac aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa
     1261 caatatgcaa aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta
     1321 gctgaaaaaa tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag
     1381 cctaacctcg atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt
     1441 ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc
     1501 actgaagatg cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa
     1561 cgcaatcagc tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt
     1621 attgagatga aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc
     1681 ttttctcttc cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg
     1741 aactaattca actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt
     1801 tgctaggtag actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt
     1861 agaagctcgt tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc
     1921 attttccacg ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc
     1981 tttctctgtc attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc
     2041 atctaaaatc aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct
     2101 tttagcctct ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt
     2161 aaaatgaaat ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt
     2221 tgcattaaaa acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc
     2281 tttctcttcc tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc
     2341 taattttttt atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat
     2401 gtcataagtt ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat
     2461 acaagtcaca ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata
     2521 gacacgtcgc atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga
     2581 ttcaagagtg tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata
     2641 caagtctagg ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat
     2701 tagcatcaca actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa
     2761 aatggaatgc ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa
     2821 cagacatctt aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct
     2881 ttcataaatc tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg
     2941 tttcatttct tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag
     3001 ttcatactct ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat
     3061 cggaagctca gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt
     3121 atatcctcca aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt
     3181 ctttttcaag tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa
     3241 tagaaagaca atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc
     3301 aaacgcagaa ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact
     3361 atcatcttta ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc
     3421 cagaataatt actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt
     3481 gcttggaata aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc
     3541 tctatagtta tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt
     3601 ctctaataat ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg
     3661 tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc
     3721 tttattacta tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac
     3781 tatcaatatc agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt
     3841 attgagaaac atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct
     3901 gatagtccat atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca
     3961 ctgtaattat ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt
     4021 gtagagacat atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct
     4081 tcgtattttt tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat
     4141 gattaccagt tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt
     4201 gtaaaattta tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa
     4261 tatgataaaa cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat
     4321 aaaagctata agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa
     4381 cggtcaatta attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat
     4441 accagagcga ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg
     4501 tggcaatgta gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga
     4561 tggtataaca tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt
     4621 acactctaat ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga
     4681 atttattgct aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat
     4741 cttaaaaata gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct
     4801 tttcctcact agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat
     4861 tcaattgaaa gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa
     4921 caagctagaa caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg
     4981 aggtgatacc atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca
     5041 aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt
     5101 gccaccgatc cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa
     5161 caagaagctc aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag
     5221 aaagcacctg atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc
     5281 agtgttaaag cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca
     5341 gcatttggta agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat
     5401 gaatggcaca gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta
     5461 cgctacgggg ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca
     5521 cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta
     5581 ctggatgctt ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca
     5641 tacacaggta tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt
     5701 aataaagaac tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt
     5761 caatctccga aaacaagagc ttctagacga gttatctctt tagatactga gactctttct
     5821 atcctcaaca actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact
     5881 agctctaaag agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact
     5941 gttaatgatt ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat
     6001 gggtttagac atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt
     6061 caacaacgat tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca
     6121 aataatgcac ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag
     6181 agctggtcaa aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta
     6241 tat
//
LOCUS       DAZKCC010000116         6081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000116 DAZKCC010000000
VERSION     DAZKCC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.119"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACG8VH_002734"
     CDS             <1..231
                     /locus_tag="ACG8VH_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111228.1"
                     /translation="KKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVDAMNVFVRA
                     ANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            691..1071
                     /locus_tag="ACG8VH_002735"
     CDS             691..1071
                     /locus_tag="ACG8VH_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9111229.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1053..1967
                     /locus_tag="ACG8VH_002736"
     CDS             1053..1967
                     /locus_tag="ACG8VH_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9111230.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            2212..2901
                     /locus_tag="ACG8VH_002737"
     CDS             2212..2901
                     /locus_tag="ACG8VH_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111231.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(3022..3108)
                     /locus_tag="ACG8VH_002738"
     CDS             complement(3022..3108)
                     /locus_tag="ACG8VH_002738"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9111232.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3784..4734
                     /locus_tag="ACG8VH_002739"
     CDS             3784..4734
                     /locus_tag="ACG8VH_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9111233.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4755..5288
                     /locus_tag="ACG8VH_002740"
     CDS             4755..5288
                     /locus_tag="ACG8VH_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9111234.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5304..5474
                     /locus_tag="ACG8VH_002741"
     CDS             5304..5474
                     /locus_tag="ACG8VH_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111235.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5878..>6081
                     /locus_tag="ACG8VH_002742"
     CDS             5878..>6081
                     /locus_tag="ACG8VH_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111236.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDL"
BASE COUNT         2267 a         1069 c         1093 g         1652 t
ORIGIN      
        1 aaaaaaaatt ataataagct aaagactcct gctcaaattg catcttttat tcctattggt
       61 ggagttggaa catgggttat tacaaatgct tttagtagtg ctgttgatgc aatgaatgtc
      121 tttgtaagag ctgcaaatca aggaaaaggt gtacaattaa catataatgc tcactttagc
      181 aatacaacat catatcaata caatgattac gctcgttatg taataaaata atcagaaagt
      241 gaaactatct catgaataaa aagattttag ctgtaattat ttcaataata gttttaatca
      301 ctatgttctt catatttagg ctaattttca atataagctt acaaaaaagt attctttatc
      361 taattcctat agcatttatc ttagcaattt ttagaagtat ttacggaaat aaaaactcta
      421 aatagctaaa aataaatcat actaaatttt acaaaaagtg gctagcattt gctagccttt
      481 tttgtatggt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
      541 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
      601 ccttttagtg agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg
      661 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aacatcaaaa tttggctagc
      721 gatcattcta aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc
      781 gaatccgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct
      841 ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt
      901 gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgccaag
      961 tggtgcaata cacaccaaca cgacgttgct cctgatgatg ctcaacgttt atccgaaaac
     1021 ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta agtcgaacca
     1081 cttcatgtag ccgagcaatc aattacgcag aaccacgagc aaccgtcaaa acaggaataa
     1141 actgtgacat tgattatgcc aaaagtgaaa tgaaacaaat acgcacgctt tatggaaaag
     1201 acgatcgtgt acaagcacac ttattgattc agtcctttcg accaggagaa attaccgctg
     1261 aaaaagccaa tcaactaggg gcagagtatg cgaaaaaaat agcccctaac caccaaatcg
     1321 cgatttacac acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta
     1381 atcttgaaac tgggaaaaaa tttcaagccc atgggcaagc atttttaaac aaatgttatg
     1441 acctaaatga cgagatttgt cttgaacatg acttaacaat cacggaacga ggaaagaaaa
     1501 ccgaaaaacg aaccatgtct gaaattaaat taaaagaaaa aaatgaaccg gtttggaaag
     1561 acgaaattcg gtttgcgatt gatcaaacaa tgaaaaacaa aaaaactcga acgtataccc
     1621 aattttgtga ttcacttaaa acattcggca ttcattgctt gaatcgcgga aaaaatttta
     1681 cttacgaatt ggtccacaaa aagaaaaaag tacgatccaa caaactagga aaggattacg
     1741 aaaaggagac gattttacat gagctggaca gacgagaacg aacctacaat caacaacgaa
     1801 ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag
     1861 aacaacgaac ttttacgcct gaacaatcgg aaattagacc gactcgaaga aaccatgaac
     1921 aacacgttga acgaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac
     1981 atcgaaaacg aaataaagag acaacgtgcc tttcttgcgc cttataccca agaaaggata
     2041 caaactacga tggattcaca agtcaaaaaa ctccaccaag cgctattaga ccagcgaaac
     2101 caagaacaaa aaaaggcttt aattcaacga tcgatggata ttcttgcccc cttatgttgc
     2161 ttagtactaa cattattagc cttttggttg gtatggggag aataaacaga catggcattc
     2221 tatttaccct atctactcat ttctgccagt attttaggaa ttatttggct tagctataag
     2281 gcttatcaaa caagatattc attaaaaggc tctaaaattc gatttaagcg atttttattg
     2341 gtcggggtta tatttgcctt atcaatcgtt ttaagtggtt tattcggcgt tttagagggt
     2401 aataaacgtg tttcaagaag cattttatta ggggatatca ctcaaaagta tgaagcagca
     2461 tgtgataaaa agaaaaaaga gcaagcatta gcacaaaaaa tggaggaatt taccgcttgt
     2521 tatgaggaca tgaacaatat ttttgtggat caagagaaac gtttaacaga taaaaacatg
     2581 aaaaaactca ctcgttttta tcaaaactta ccagaaaaac agcaaaaaga ggtacaagaa
     2641 aaatatgagc aagtaaaaaa agacgtgcaa tattttaaag atacgcagac agaagaagct
     2701 tgttgtaact tatttagcga tacaatccct ttttcaacca gtgaacaaga acgaaaagaa
     2761 cggcaacaat cggtcacata cgaacgttat gaaacactgc ttcagcaagc aacaaacata
     2821 caaaatccaa cgaaaaaaga aacggcatta aattatctag aaagtgtcaa ggaatggttg
     2881 gatcagcaac aacaaaattg aatttgatta gcaatcatgt taaactgaac ctgcaaatga
     2941 tcaaacatta gatacacgaa agccccagag aggtcggatt ctctggggct ttttttcagg
     3001 ttgatgtcgc ctttattgat tttacttctt gtttcggtta ttcaaccaat aactgaataa
     3061 agagacacaa acttctacaa gaagggggca aatgaaccgt tcaaacatta gatacacctc
     3121 cttccagtcg caagactgtc ggataggcga caagttatta taccttacaa aataaaatct
     3181 gtgtggtgtt tttaagtatc ccttattttc gggttgttag ggcttgccct gaccgcctgt
     3241 aaggcgcttt aagaacaaga taaggttttt tacccaagac tgtcttaaaa cgacttagac
     3301 gagctatcag aggcttcccc cacctccgtt actgacttgt ttttagcaaa ggagccttta
     3361 ggcgactgcg cagtacaatg tgagagcata gcgatcacac ttttttagcg accgtaggga
     3421 gcaaggtttt gaattggcag ttttgaaaaa tgcgcgaacc tgttggtaga aaaaagaagt
     3481 ggtttttagg cgggaaattt tgtcaccctc ttttataatc tctttttaaa ccttttttta
     3541 aacctcttta ccgtcggctg tgtccttact ccttcaatgg atacagcgtt ttataaacta
     3601 cattttgtct gcttttaaac tacattttgt ctgcttttaa actacatttt gtctgctttt
     3661 aaactacatt ttgtctgctt ttaaactctt tttttgcact cacgtatgtt ataatatgca
     3721 ttatcacaga caaaacgttg tttataacgt ctgtatccat tgaaggagta aggaggaata
     3781 tctttggcaa atgaaatcgt gaaataccat catgaattaa ataccattcc gttgcggaaa
     3841 tttacaccag ttgaaatgaa tctatttttc tctattgtgt ctagaatgcg cgaaaaaggc
     3901 gataaaacag tccgtcttac ttttgaccaa ctaaaagatt taagtaatta taaagcaact
     3961 gctaataatc gtttcattaa tgatttagaa atgacttatg ataaattaat ggatttacgt
     4021 tttggtaaaa gaagtgctaa tgggctcgat atagatagat tcgttatgtt tagtgagttt
     4081 aaaatacgtg gaactgctaa agatccatat gtagacatac aagtttacga aaaagcttta
     4141 cctttactaa acaatttaga tgagtgggtt cgctattcac tccaacaatt taacgaatta
     4201 caaagtagct atgcaaagac tatgtttcga ttactaaaac aatatcgaac taaaggtttt
     4261 gcttattttt ctaaagagga ttttcatgaa ctattagata ttccaaaaag ttactggaac
     4321 aaccaagcaa acgttgataa tcgtgttata aagcctatac gacaggaatt aaccgcaatc
     4381 ttcaaaggat tatcaatcaa aaagaaatac ggaaaagggc gaggaaaacc tgtcgttggc
     4441 tatcaattta cgtttaaacc agaaatcaaa aaagcagatg attttaataa acatgcacca
     4501 accaaagaag aacaaaataa agcattggct ttagctgata aacaaaaaga atacgaagaa
     4561 cctgctacaa caattaacga aaaaatcaaa cagaagaaac gagaacttga actcctccaa
     4621 caaatcaaac aactagaaaa agagaaagaa gctcttgaaa aacaaacaga gtccgcactt
     4681 tcgaaaaaag agaaaaaaag tttattatct gatttagata atttattcca gtagcaacct
     4741 aggaggcaac cagtatgcaa ccacaagaaa cctttacaat aaaggaattg tcagaacttt
     4801 ttaagatgag taggcaagca atatcgaaac atatccaaaa attagattct tccatgatag
     4861 ctaaaaatga gcgtggttat aaggttgtat tgcgttctgg agtattgcaa cttgctcgca
     4921 acttaggcaa ccaaatgttg ctagataggt tgcacgagta cgaacaagac aacgataaaa
     4981 atgttaatat accaagcaaa aacgtggtta caaacaagtt gctagatcag ttgcgtgaag
     5041 aaaatgaatt tttacgagaa caacttgccc tcagagagca aaataattct catatacaaa
     5101 aactactcga ccagcaacaa caactcaccc tacaagccaa caaacaaatc gaaaaactgc
     5161 aagaaaaact acagttgact tatacagaag aagctccaga gaatgactca acgactcttt
     5221 ctgaagcaac agaaacaata gacaaacaaa ttgatactga aaaaaaatgg tggcaacttt
     5281 ggaaataact ggagggaaca ttcatgacaa gaaaaaatct tctattcggt tctattttac
     5341 taggaattct aggattcagt ccgatgttat tgtcagtgat caataaaggg aataaacatg
     5401 gtgaattgaa attgaatcca aattattaca acagtcctaa cgaagaattg tttgtaaaag
     5461 aaacagataa ataataaaaa agactgctct ttcgagcagc cagttaaaaa ataatgagtg
     5521 tttattcttg aaaaagcaac gcgcttatga agcgctacca aaaatttatc atattttatt
     5581 aaaaataaca acccagtaaa aaatgactgg taaaaattca caaagaaacg gaaagaagca
     5641 accgcagaaa atcaagctta aaaagagctt ttgacctaga ccaatcgaaa atcacaaagt
     5701 gagcctctgt atcggttgat acaagggcta cttggcatag tgtacattat gtctactaac
     5761 ctttaaaata acacgtttta taaaataaac cctcttgaca atatttaatt ttctgattat
     5821 tctaaatata tacagaaagc gatttcctag gattttatat ctaaggagga agtaacaatg
     5881 aaaaataaaa ttattaaaat tattacttgt ctatttttgt tcggtatgat tgttggagca
     5941 agtactccgt taaatctgtt tccttttagt gctagaggta taaaagtgga agctgcaagt
     6001 tcacgatata atcataatca tcgtggattt acttgctata aaatgaacta ttatgttact
     6061 aataaaatgt gtagagattt g
//
LOCUS       DAZKCC010000117         6066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000117 DAZKCC010000000
VERSION     DAZKCC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.120"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8VH_002743"
     CDS             complement(254..541)
                     /locus_tag="ACG8VH_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9111237.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8VH_002744"
     CDS             complement(561..785)
                     /locus_tag="ACG8VH_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9111238.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8VH_002745"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8VH_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111239.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8VH_002746"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8VH_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9111240.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8VH_002747"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8VH_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9111241.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG8VH_002748"
     CDS             3560..3646
                     /locus_tag="ACG8VH_002748"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9111242.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG8VH_002749"
     CDS             complement(3767..4456)
                     /locus_tag="ACG8VH_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111243.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACG8VH_002750"
     CDS             complement(4463..4741)
                     /locus_tag="ACG8VH_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111244.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACG8VH_002751"
     CDS             complement(4701..5615)
                     /locus_tag="ACG8VH_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9111245.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG8VH_002752"
     CDS             complement(5597..5977)
                     /locus_tag="ACG8VH_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9111246.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DAZKCC010000118         5773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000118 DAZKCC010000000
VERSION     DAZKCC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.121"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..2254
                     /locus_tag="ACG8VH_002753"
     CDS             101..2254
                     /locus_tag="ACG8VH_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFP9111247.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2223..3638
                     /locus_tag="ACG8VH_002754"
     CDS             2223..3638
                     /locus_tag="ACG8VH_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9111248.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3671..4705
                     /gene="sppA"
                     /locus_tag="ACG8VH_002755"
     CDS             3671..4705
                     /gene="sppA"
                     /locus_tag="ACG8VH_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9111249.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4707..5228
                     /locus_tag="ACG8VH_002756"
     CDS             4707..5228
                     /locus_tag="ACG8VH_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9111250.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5225..5611
                     /locus_tag="ACG8VH_002757"
     CDS             5225..5611
                     /locus_tag="ACG8VH_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9111251.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2068 a          780 c         1089 g         1836 t
ORIGIN      
        1 tcaaagtttt tgtggaatta tgagacaata ttttcactgt attaaagcta tattgagaat
       61 ttttctataa aaatttttta gaaggagttg tgttagataa gtgataaaat catttccttt
      121 tgtgcagcaa caagatgaaa aagattgtgg tgttgcttgc ctttcgatga ttttaaaata
      181 ttttaaaact gatatcccga ttcataaatt acgggaccta tctgggactg atttagaggg
      241 tacttcagca tttggattga aaaagacatt tgaaaaattg tcttttgatt gtatggctat
      301 tcaagcagat agtgagatct ggaaagaaag agatcttatc ttaccattaa ttgcacatat
      361 attggttgat aacagctata tgcactacgt tgtaatttat aaaacgaagg gaaacgtact
      421 atggattgca gatcccgcta atggaaaaaa tagaaaaacg attgaagcgt ttcaaaaaga
      481 atggacaggt attcttttat tacctatgcc tagagaaact tatattccta ttaaagaaaa
      541 agtagctgga ttaagttctt ttttgcctat aatttggaaa caaaaaggat tagtattcca
      601 catcgtttta gcatcgattt tcattacgtt ttttggcata ggaagctcat attattttca
      661 aggtattctt gattttttca tccctaatca agcacgttca actttaaata taatttccat
      721 tggcttgatc gctgtctatt tatttcgggt attgtttgaa tatagtcgta gttatcttct
      781 tattatttta ggacagcgaa tgagtatggc aattatgctt cagtatttca aacatgtgtt
      841 aactttacca atgaattttt ttgctacgag aaaatcaggg gaaattattt ctcgttttct
      901 tgatgccagt aagattattg atgctttagc aagtgctacg ttatcaatat ttttagatat
      961 tggtatggtg ttgcttgttg gggttacgtt agctattcag aatagtacgt tgtttttaat
     1021 cactctatgc tctttgcctt tttatgtagt tgcaatattc gctttcgtga aaagctatga
     1081 aaaatcgaac agggaagaaa tggcagccgg tgcagctgta aattctagta tcatagaaag
     1141 tcttaaagga atagaaacaa ttaaagcata tagcggtgaa gagaaagtat ataaccgtat
     1201 agaccatgaa tttgttaaat taatgaaaaa atcatttcgt acggttacgc ttgataatat
     1261 tcaacaagga attaaacatt tgattcaatt aattagcagt gcattaattt tatggctagg
     1321 ttcttatttt gttatggata atagaattag tctaggtcaa cttatcacat acaatgcttt
     1381 actagtattt ttgacagatc ctttacaaaa cattattaat ttacaagtga aaatgcaaac
     1441 ggctcaagtt gcaaataatc gtttaaatga aattttcgcg attgattcag aaaaatcgaa
     1501 ccagtctttt gaatatatgc tttctgaaca attatttcaa caagatatca ctgtaaaaaa
     1561 cgtttcattc tcgtatagca tgaaggcccc aacgttgaac gaaatttcat gtacaattcc
     1621 ttcgcacagt aaaacggcta tagttggagt tagtggttct ggaaaatcaa ctcttgcaaa
     1681 gttatttgtt cgtttttatg aagcatcaga aggatcgatc cattatggaa acattaatca
     1741 cttagacatt ccttttcaga tattacgcga tcgagtgaca tatttgcctc aagagtcatt
     1801 tttctttagt ggaacaatca ttgagaatct tatattcggt ttagatagcc caccaagttt
     1861 cgaacaaata atagaagttt gtgagatagt ccaactaaat gaatttatta atcaacagcc
     1921 attacgttac gagacattgt tggaagaagg tggaaataat ctttcaggag ggcaacgaca
     1981 acgattagcc ttagcacggg ctttattaaa aaatccggac atattaattt tagatgaagc
     2041 aactagtgga ttagatacct tgttagaaca tgcaattatg aattatctcc tcaatttaaa
     2101 agaaaaaacg attattttta ttgcgcatca tttaccaata gcaaaagcat gtgatcaaat
     2161 tttagttctc catgaagggc atttggtaga aaaaggaacg cacgatgaac ttcgttttgc
     2221 cgatggtatc tatcaaaaat tatgggaaat ctaggagagg gaaacaattt atgcagaata
     2281 ataattggtt cgaacattct agtatctata ctaaacaaca tcatagcttc taccgttggg
     2341 tgttataccc tgtaattctt tttttatgta ttattggatt gtttttaacc tttgctaaaa
     2401 aagaggttgt tattcggaca acggctcaat tgaaccccga aaaaatagag aagttacaag
     2461 tgccattaga agctaaaata atggaaaact tcctaaaaga aaatcaattt gttcataagg
     2521 acgatgtgtt ggttagattg gattgttctt taatagagaa tgaaaaggct cagatagaac
     2581 aagaaaacca acgtattact caacaaataa agatggctca gctatttatt gaaagtataa
     2641 gtaaaggaaa aaatttgttt tcaacggatg acagttttgg ctacagtaat caattaaaga
     2701 gcatgttgtc agaaaaagaa tcactccgct acgctttgaa gcaaagtgaa ttaaatgatc
     2761 aaaagcaatt agaagtatac gaaaagacaa aaagacaact agaaaaacaa attgagagtt
     2821 cagatagtaa attacaagaa tggcaacaag tacaggtagc ttggagtaat aatcaatcat
     2881 taaaagattt ttcaaaagaa atgatggcaa actatgagaa ttggcaagaa caactaaata
     2941 atgtttctga tgatcaaaaa aatcaagtga aactgacaat ttcagcaagc ataaatgaac
     3001 aaattgagca actaaaaaaa gaagtagaac agtatcagtc agaaaaagct aaattagtta
     3061 aaccaactac ttctgagaat gacagaatta gtcaaacgga aaaaggaaag caagagctag
     3121 aacaagcaat aacaactgta aaacaaacag ttacagagct aaatgaaaaa caagagaaga
     3181 accagtcagt tatcaaaagt ttggacgatc aactttcaaa aggaatacta aaagctcctg
     3241 tatctggaac ggttcattta aatgaagaaa caaagggaca agcagaacta gccaaaggaa
     3301 cagtgcttgc agaaatttat ccgatccacg aaaaatcaaa aatgaaattt acagcacttt
     3361 tacctgcgaa tgaaagtacc tatattaaag aagggatgaa ggttcatttt aaattggatc
     3421 aaaaaggtgg ggctccgatt acgatcgacg gaactttaga tgaaatttca gaaaattcta
     3481 ctgccactga gaaaggtgtt ttttatgcag tgaaaggaac gttaaaaaca actcaatcgt
     3541 ttccgtttcg ttatggatta actggagagt tgtctttaat tgtaggagaa aaaacatatt
     3601 ggcaacaaat tgtcgatgtg tttctaaata aaaaataatt atttttacta agaatatagg
     3661 aggagtttat atgaataaaa gaagatggat tgctgtggga gtagcgtgcg gattattgtt
     3721 actatccatt atcgtcgcgc taattccagg aaaagataaa gaggaagcat caaatacaac
     3781 cctaacagga ataaataaga ttttttacgg atctaacgaa atcacagaag aaacgttaga
     3841 agaaggcgca tcaaataaaa agattgtcaa actatccgtc aatggagtta ttgcagatac
     3901 aggtgaaagc aatcttttta gtagggaaca atataaccat cagaattttt taacacagct
     3961 aaagaaaatt caagaagaca aagcggttaa gggagtgtta ttagaagtaa attcacctgg
     4021 gggaggaata tatgaaagtg ctgagattgc taaagaaatg gcaaaaatta aaaagctaga
     4081 tattcctatc tatactgcat ttaaaaatac agcggcaagt ggagggtact acatttctgc
     4141 tggttcagat aaaatatttg ctacggaaga aacaacaact ggatcaattg gtgtcattat
     4201 ttctggttta aattattctg gcttattaga aaaacttggc gtaactgatg cgacgtataa
     4261 aagtggtgca ttaaaagata tgatgtcacc tcaacacaaa ccttcagagg aagagaataa
     4321 agttatacag gaatttgtga tgagtgctta tgatcgtttt gtcaatgttg tagcaaaagg
     4381 aagaaatatg gatactaatg cagtgaaaga acttgcggat ggaagaattt atgatggtaa
     4441 ccaagcagta gaaaatgggt tggtggatca aattggttac tctgaagatg cattagattc
     4501 cctaaaaaaa gagaagaaat taactgatgc gacggttata gaatataaaa atgacacaac
     4561 tggctttgct tcttcttggt taggtaataa gattgctgaa tggcaagggc taaaggcaac
     4621 tacatcagat cgtgtaattt cagtttttga aaaacttgga ttttctgata ctccaaaacc
     4681 aatgtattac tatggagggc tataaaatga ataagaagag tggcattgaa actttgccta
     4741 attatgtatt tgcgggattt tggttacgat tttttgcttt tcttattgat ttaatttgta
     4801 ttagcatcat cacccatgct actataggtc tcttttacaa aatcatggga ctggaatctg
     4861 acaaatccat tattactttt tatggtatag cgtcattagc tatttattta acctattttg
     4921 tacttttgac taagctgaat tcaggacaaa caattgggaa aatgattttt gggataagag
     4981 tagtgagctt tgaggaaaaa gagctaagtt ggcaaactat ctttattcgt gagtgtatat
     5041 gtaggtttat attaaaaaat tttgtatttc aaattgggta tttggtggct gctttcacgc
     5101 ccaagaaaca acatattggg gattattttt ctaacacgtc agtggttgtt attcgttcat
     5161 tagaagctat taactatggt caaaaaattt ctaattctaa aacacttaat ctggagagaa
     5221 ttaaatgaat aagagaaaaa cagagtttat tttgatttta attagtggaa taacagggat
     5281 tttatgtagt cttttagcat tgatgggtgg tatgtttcta tcttctattg attcaactac
     5341 tttaaatact acagaactaa ccagtaagga tatagatatg ttagtcaatc taagcgggaa
     5401 tgttgtttta tttggaggaa ttaccctagt tttttcgata atgattttca taataggttt
     5461 tttagtaaga aaaaatcaaa aaacagtggt ctatggtatt agtttactca ttttatctat
     5521 tattccattt tttcttttaa cattattgtg ggtaattcct ggagttttag gtattattgc
     5581 gggattgatg cttctaataa gaaaatctta attaggcaat gtgtagacaa cgtgaaacga
     5641 ttaatcaatt aaaagctaaa tacgttttga gaaatgataa cttgtagtcg atggaagaca
     5701 tgtagataaa aaaactacgt gtcttttatt tattctaatt ttttcgaaaa aaagacactc
     5761 tctgattgtt gta
//
LOCUS       DAZKCC010000119         5769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000119 DAZKCC010000000
VERSION     DAZKCC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.122"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8VH_002758"
     CDS             complement(<1..300)
                     /locus_tag="ACG8VH_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9111252.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8VH_002759"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8VH_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8VH_002760"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8VH_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111253.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8VH_002761"
     CDS             1706..1942
                     /locus_tag="ACG8VH_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111254.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8VH_002762"
     CDS             1949..3901
                     /locus_tag="ACG8VH_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9111255.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8VH_002763"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8VH_002763"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9111256.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG8VH_002764"
     CDS             complement(4797..5489)
                     /locus_tag="ACG8VH_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9111257.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DAZKCC010000120         5749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000120 DAZKCC010000000
VERSION     DAZKCC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.123"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1168
                     /locus_tag="ACG8VH_002765"
     CDS             14..1168
                     /locus_tag="ACG8VH_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP9111258.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1141..1584
                     /locus_tag="ACG8VH_002766"
     CDS             1141..1584
                     /locus_tag="ACG8VH_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111259.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2696..3664
                     /locus_tag="ACG8VH_002767"
     CDS             2696..3664
                     /locus_tag="ACG8VH_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9111260.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3674..4162
                     /locus_tag="ACG8VH_002768"
     CDS             3674..4162
                     /locus_tag="ACG8VH_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9111261.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4458..4739
                     /locus_tag="ACG8VH_002769"
     CDS             4458..4739
                     /locus_tag="ACG8VH_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111262.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4741..5157
                     /locus_tag="ACG8VH_002770"
     CDS             4741..5157
                     /locus_tag="ACG8VH_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111263.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2147 a          982 c          937 g         1683 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caagcccttt
     1801 agtgtgtttt gtagaaaggg accttatagt cactttcaca ttacaaacga tacgtcctat
     1861 ggacgtatca aaagggtggg acttatgagc aaattaaagc aaaatttggc tagcaatcct
     1921 tctaaaaaat acacctattg atcagagtca aaataaatca gttttcgagt gagcgaatcc
     1981 aaatttgcaa ctctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa
     2041 cattagtcga tatatacata gggctctaat ttgcgtttta aggcgtttta aacgttctgg
     2101 tatgtaatta taccaaagcg actttaaaac gccttaaaac gaattttaga ggttctgtag
     2161 aacgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttta aaaacaagat
     2221 aagggatttt atctagactt gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa
     2281 ggactcctta cgaactgttt ttaagcaaag gagcctttag gcgactgcgc agtttaatgt
     2341 gagagcgtag cgatcacacc tttaaagcga ccgtagggag cagattccca gtttaggaaa
     2401 tttcgcgaac ctgtttggag aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc
     2461 ctcttttata atctctttta taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt
     2521 actctcccaa ggtgtgcaga atcttttagg ggtctttttg tctgttttta ggggtctttt
     2581 tgtctgtttt taggggtctt tttgtctgtt tttaggggtc tttaattagg gggttgaccc
     2641 ccattagaaa gctctttata attttgttaa gtagttaatc ttgaaggagg attttatggc
     2701 aaatgaaatt gtgaaatacc accatgaatt aaatacgatt cctttacgca aatttacgtc
     2761 agtagaaatg aatttattct tctctataat atctagaatg cgtgatgtag gcgataaaaa
     2821 agtacaattt acattcgaac aattaaaaga tttaagcaat tataaagcaa ctgcaaatgt
     2881 tcgattcata gatgatttag aaacaacgta tgacaaatta atggatttac gttttggtag
     2941 aagaagtgct gacggtttgc aacgtgaacg atttgtttta tttaatcaat ttaaaatcga
     3001 tggaaaagct gatattcctt ttgctgaaat acaagtccat gagaaagcct tacccttact
     3061 taataatctt gaagaatggg ttcgatattc tctccaacaa ttcaatgaat tagaaagcag
     3121 ttattccaaa accatgttta gattattaaa acaatacaga accaaaggaa ttgccaattt
     3181 ttcaaaagaa gactttcatg aattacttga tatccctaaa agttatagag aaagtgatat
     3241 aaataaaaaa attataaagc ctattcgcca agaactaacc gcaatcttca aaggtttgac
     3301 aatccaaaag agatacggaa aaggacgggg aaagcctgtc attggctatc aatttacatt
     3361 taaacctgag ataaaaaaat cagacgactt taacaaacat gctccaacca aagaagaaca
     3421 aaataaagct ttggaactag ctaatcaaca ggttcaatct gaaaaagaac ctacttcatt
     3481 acaagaaaaa atcaaagaac aaaaacgaaa attagaactt ttacaacagt tagcagaatt
     3541 agaaagaaaa caaaataccc caagtaagaa aattaacaaa caaatagcag ataacaattc
     3601 cgatattacc ccagaattta aagaaaactt actagataat ctccaaaact tatttaaaga
     3661 ataaggggtt tttatgacag ataaaactat caaagaatta gcagatattc taggtgtttc
     3721 aaaagataaa gtgaagtatc aactgaggaa attacctagt aattttacct acaaaaaagg
     3781 aaatattacc tacctgaaaa aagaagcagt aacaacaatt acaaaggttt tatcaggtaa
     3841 tgaatcgggt aacttaccta gtgaattacc cgattcatta cctaccgatt acctactcaa
     3901 ggagattgaa actaagaata atcatattga agaaaaagat aggcaaattg aaaaacttca
     3961 aatagcttta gaaacgcagc aaaaattact cgaccagcaa caacaactca ccctacaagc
     4021 caatcaacaa atcaaaaaac ttcacgaaca actccagcta acctatgcag aagaatcttc
     4081 agaaaaagaa cccctttctg ttactgaaga aactcaaaaa ggacaaaaca aggagaaaaa
     4141 atggtggcaa ttctggcaat aacccaacaa tttttattca taaaaattgt tgggttactt
     4201 tctttttttg cttttacaag catagcgaca tgatctcacg tcgctatttt ttaaagagca
     4261 taaggaaacg cggtatcact ttcttacgaa aggtgctagg taggctgtgc ctaccacgtt
     4321 tctaagaaaa aaatttgcta tcactttttc gtcattgtgg atagcttatg tatagtgttt
     4381 cggcaaaaaa cagctataca aatactatat ttgtatgata tacataaatt atacacaaat
     4441 gcgaaaggaa ttcttctatg gacattttag ttcggaatat tgatccaaaa gttatttcag
     4501 aaatagatag acgttgccaa gaactcacaa caaaaactgg caaaaaatgg agccgtaatg
     4561 cgtacctaaa agttctaatc gaaaacgatt ttgaccaggc acttaggcat tacaaaaaag
     4621 atcaattcga tttattactc gaaaaattta tcgatataca agcaaaaaat actgaaacat
     4681 tgaacgacta tgttcataca aacaaccaac tcattgaaac acttatcgga ggaatataac
     4741 atgagaaaaa cttctgttca aaaacacgtt accctttatc ccagtcacat tgacgtttta
     4801 gattccatca tcgaaaaaaa tccagatatc aataactact cggaagccat tcgttttcta
     4861 tgtatgagtt atgaacctaa tactaatacg acaactacca gcaaccaagc aaaactaaat
     4921 gcgatgggaa aagaaatttc tattatgtcc gaaatcattg cagaatttgc tgatttatat
     4981 ctaaaagata ctggtgttct tatcggtaca aattctaaag tatatcaaga agtgaaagaa
     5041 aaaattgaaa atcaactcaa tccctcccga cgaaatcaac ccaaaaatcg tcaaaaagaa
     5101 accaatcaaa cattcacttc tagaaatttt gacccaacaa actttctaaa aagatgaaaa
     5161 agaaaccgaa aattttttct atttctcagt ttctttttta gggggcagtc ttccagcaag
     5221 atttgcaagt taccggtaac ttgcacaaaa aaagaaaaat ccgcttgtgc gaatccttct
     5281 ttttgcattt tggggcgctg ccccaatctc caaatatcgg ttatttaata gcgtggtaac
     5341 tcaaaaaaat agaacgtact ctgaaataaa aaaagactgc tctaacgagc agccaataaa
     5401 aaataatgag cgtttattct tgaaaaagca gcgcgcttat gaagcgctgc caaaaattta
     5461 ccatatttta tgaaaaataa caagtttcta aaaaggaatc ggttaagatt cacaaagaat
     5521 cgtaaacaga caatagcgga aaatcaagct tccgaacgta ccttttagct agaccaatga
     5581 aaaatcacaa agtgagcccc tgtatcggtt gatacaaggg ctacttggca tactcgacat
     5641 tatgtctact agctcgataa ataacgatta tgttcccaaa acaatatttt atgaactata
     5701 acaaaaattt gttatcgcat attgtatatt ttaattatag gtgtatatc
//
LOCUS       DAZKCC010000121         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000121 DAZKCC010000000
VERSION     DAZKCC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.124"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8VH_002771"
     CDS             complement(488..1297)
                     /locus_tag="ACG8VH_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9111264.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8VH_002772"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8VH_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9111265.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8VH_002773"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8VH_002773"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9111266.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8VH_002774"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8VH_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9111267.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8VH_002775"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8VH_002775"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9111268.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8VH_002776"
     CDS             4179..4913
                     /locus_tag="ACG8VH_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9111269.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8VH_002777"
     CDS             4897..5607
                     /locus_tag="ACG8VH_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9111270.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1833 a         1194 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcataaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKCC010000122         5642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000122 DAZKCC010000000
VERSION     DAZKCC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.125"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8VH_002778"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8VH_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9111271.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACG8VH_002779"
     CDS             complement(1164..1874)
                     /locus_tag="ACG8VH_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9111272.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="ACG8VH_002780"
     CDS             complement(1942..2757)
                     /locus_tag="ACG8VH_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP9111273.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACG8VH_002781"
     CDS             complement(2768..3736)
                     /locus_tag="ACG8VH_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9111274.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5642
                     /locus_tag="ACG8VH_002782"
     CDS             4499..>5642
                     /locus_tag="ACG8VH_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9111275.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQ"
BASE COUNT         1734 a         1157 c          900 g         1851 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aa
//
LOCUS       DAZKCC010000123         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000123 DAZKCC010000000
VERSION     DAZKCC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.126"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8VH_002783"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8VH_002783"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG8VH_002784"
     CDS             complement(580..1353)
                     /locus_tag="ACG8VH_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9111276.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG8VH_002785"
     CDS             1497..1847
                     /locus_tag="ACG8VH_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9111277.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG8VH_002786"
     CDS             1828..2544
                     /locus_tag="ACG8VH_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP9111278.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG8VH_002787"
     CDS             2544..3992
                     /locus_tag="ACG8VH_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9111279.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG8VH_002788"
     CDS             complement(4062..4571)
                     /locus_tag="ACG8VH_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111280.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG8VH_002789"
     CDS             complement(4561..5286)
                     /locus_tag="ACG8VH_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111281.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKCC010000124         5341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000124 DAZKCC010000000
VERSION     DAZKCC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.128"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..284)
                     /locus_tag="ACG8VH_002790"
                     /pseudo
     CDS             complement(111..284)
                     /locus_tag="ACG8VH_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8VH_002791"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8VH_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACG8VH_002792"
     CDS             2090..3130
                     /locus_tag="ACG8VH_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9111282.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /gene="traE"
                     /locus_tag="ACG8VH_002793"
     CDS             3954..4286
                     /gene="traE"
                     /locus_tag="ACG8VH_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP9111283.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="ACG8VH_002794"
     CDS             4905..5246
                     /locus_tag="ACG8VH_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9111284.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          727 c          900 g         1771 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattaata
     4861 taaagcatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt
     5341 t
//
LOCUS       DAZKCC010000125         5311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000125 DAZKCC010000000
VERSION     DAZKCC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.129"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8VH_002795"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8VH_002795"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9111285.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8VH_002796"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8VH_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111286.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8VH_002797"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8VH_002797"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9111287.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8VH_002798"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8VH_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111288.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8VH_002799"
     tRNA            3917..4000
                     /locus_tag="ACG8VH_002799"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5311)
                     /locus_tag="ACG8VH_002800"
                     /pseudo
     CDS             complement(4240..5311)
                     /locus_tag="ACG8VH_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAZKCC010000126         5200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000126 DAZKCC010000000
VERSION     DAZKCC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.130"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..694
                     /locus_tag="ACG8VH_002801"
     CDS             23..694
                     /locus_tag="ACG8VH_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9111289.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="ACG8VH_002802"
     CDS             753..1193
                     /locus_tag="ACG8VH_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111290.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="ACG8VH_002803"
     CDS             1205..1873
                     /locus_tag="ACG8VH_002803"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9111291.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="ACG8VH_002804"
     CDS             1937..2560
                     /locus_tag="ACG8VH_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP9111292.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="ACG8VH_002805"
     CDS             2616..3002
                     /locus_tag="ACG8VH_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP9111293.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="ACG8VH_002806"
     CDS             3215..4606
                     /locus_tag="ACG8VH_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP9111294.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1727 a          862 c         1095 g         1516 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     4801 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     4861 aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa aatagcttct
     4921 catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca ccgtttacca
     4981 gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata taaatacaca
     5041 ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat ttattattaa
     5101 agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt tcccattttc
     5161 ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc
//
LOCUS       DAZKCC010000127         5186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000127 DAZKCC010000000
VERSION     DAZKCC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.131"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..981
                     /locus_tag="ACG8VH_002807"
     CDS             <1..981
                     /locus_tag="ACG8VH_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9111295.1"
                     /translation="YLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMI
                     GGVFWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVF
                     IPELFIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFT
                     IQGLLEYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVS
                     EFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVI
                     AIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            978..1697
                     /locus_tag="ACG8VH_002808"
     CDS             978..1697
                     /locus_tag="ACG8VH_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFP9111296.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1712..2797
                     /locus_tag="ACG8VH_002809"
     CDS             1712..2797
                     /locus_tag="ACG8VH_002809"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9111297.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2933..4465
                     /locus_tag="ACG8VH_002810"
     CDS             2933..4465
                     /locus_tag="ACG8VH_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9111298.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4643..4864
                     /locus_tag="ACG8VH_002811"
     CDS             4643..4864
                     /locus_tag="ACG8VH_002811"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9111299.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1799 a          553 c          997 g         1837 t
ORIGIN      
        1 tatttaacaa ttaaagatta ctataaagta ttcgctataa ttgaaaaatt attctggatt
       61 tcactgccac tttgtttaat tgaaagattc atggtcacct atccaactgg aacaattgta
      121 ggagatatga taggtggagt tttttggaat tattcaggtt gtaatatgcc attgaattta
      181 attttgtgtg tgacagttac tgttgttaca atcggatatt tcaagggaga aaaaaatttc
      241 atccatttta ttttatgtgt ttgtgcagca ctttttatgt ctgcattagc tgaattgaaa
      301 gtttttattc ctgaattatt tataatattt gttgcaatag tattcacttt aaaagttgat
      361 tttaaagtag tgatcaagat attatcatgc gtaatacttg tgtcgatagt attatccttt
      421 tttataacaa tatttattac attgaacgat aatggacaaa gctatgctga taatttcaca
      481 attcaaggtc ttttagaata tgcgtcaaga gattctggat atgatggtgt tggtgattta
      541 aatcgaataa ctggtatctc tagagtagta gatgaaatat ttaataataa ttttagatta
      601 gtcctaatgg gagtaggcat tggcaatgca gagtttacca atttttttgt cagtgagttt
      661 tataaatcgt ttggtaatct tcattatcag tattttcatc atatttggtt atttatagaa
      721 gttggaatat taggagtttc gatattccta ttttttataa taaagacatt tttcaatgca
      781 aaaagatctt ttatgaagga taaagatgca agtcttgtta aagttattgc aattatgtcg
      841 gtattctttt ttgtgtataa cacaacactt aggggtgaaa caacggggcc actgatttat
      901 atgattttag ccatgcctga agttgtgaaa aggaaagagc attattatag tgaaaaaaat
      961 acaaaaggag agatattatg attcctaaga agatacatta ctgttggttc gggggaaatc
     1021 cacttccgaa aaaagataga aagtgcatag aaagctggaa aaagtattgc cctgattatg
     1081 agattattga atggaatgaa tcaaattatg atttaaataa gaaccgttat atggccgatg
     1141 catataaaga acgtaaatgg ggttttgttc cagattatgc aagatttgat attatttata
     1201 atcatggtgg attttattta gatacagatg ttgagctaat taagtcgtta gatatacttc
     1261 gtgaaaatca tgcctatatg ggatttgaag gggaaatctg ggttaatggc ggtattggtt
     1321 ttggtgcaga agaaggacat tctgttatta aaggtttaag ggatatttac gattcattaa
     1381 gtttttatca aaatgatggt acattgaact tgaaaccgag tccgtattat attacagatt
     1441 atcttgaatc tattggattg gttcgcgata acagcgaaca aataataaat ggcctgcata
     1501 tctatcctac ggattatttt gctgccaagg attatgatac tggaaaaatt tataaaacta
     1561 gtaatacaat aagcattcat caatataatg catcttggaa aacacctaaa caaaaatttg
     1621 tttatagatt aaaatcaaca attggacaaa aaaaatttaa caggttactt gcactgaaaa
     1681 aatttttaca cttataaagg agaaaagtac tttgaagaaa atttgtttca ttagtggtgt
     1741 aatttctcgt tctgggggaa ctgaaagagt agggagtatt attgccaata gcttgtctga
     1801 tttaggttat gatgtcacta ttttaagctt ttggaatagt ggagagcctt attttgggtt
     1861 agataagacg attaaggttg attatatgtt aaatcctaaa acggagggaa aattatatag
     1921 aacttattta tatccggttt ggaaattgca caagtatata tgcaaaaatg aatttgatat
     1981 agtaattgat attgatactt tgttgtcttt gtatactaca agggcaatta aaggaacaaa
     2041 gtgcaaattg atttcttggg aacattttaa ttactggaca atggaaatgc tgaaagaaaa
     2101 gaagagattc aaggctaaaa aattgataaa aaaatatgca aagaggcttg tggttctcac
     2161 agaagaagat cgtcaaaaac atattgatga atatagttta tcagaagatt ttgttataac
     2221 tatgccaaat ccgtgtatat ctaatatcga gtacgaatat cattatgata aaaagagatt
     2281 tcttgcagta gggcgattag tggaagagaa aaattttgct gacatgataa gtgcttggaa
     2341 aattgtagaa aaaaaagtag atgattggga tcttatcatt gtaggaaaag gtgaattaga
     2401 aaaagatctt cgccagcaga taaaagattt aaatctgcaa agagtaacta tagcagggca
     2461 ttcagaccat gtggaggaat attataaatc tgcatcttgt tatgtgctta gttctgttta
     2521 tgaaggattt cctatggtta tattagaagc acagtcttat ggattaccag ttatttctta
     2581 tgattgtaaa actggtccga gagatttggt acatcataat tttaatggga tgttggttga
     2641 agataagaat atagaccaat tagctaagag catgataatg tttactaaga atactgattt
     2701 agcaatgaga atgtcactga acgcatataa taatgtacaa aaatttaatt tgaaagaaat
     2761 tacaaaacaa tgggttgctt tgattgaaaa tgtatagttt tttgtggttg tccaaacatg
     2821 aaaattagcg taattaatca aagagctaga agaaacctat tacatttcaa gattttagaa
     2881 attatatagt attaggtaac aataaaaaat agaaagtttg gagccatatg atatgataat
     2941 taatcgtaaa caaaatgcta aaaggaatat aattactgga acaattttta agttatatca
     3001 gattatcatt ccctttttta tgcgtacact tatgatttat cagatgggaa tggagtatgc
     3061 tggattgaac aacctgttca catctatatt tcaaatgtta aatttagcgg aattgggaat
     3121 aggtgctgca ctaactgtaa gtatgtataa acctatagca gaagacaata gaaaagagat
     3181 ttgtgcttta ctgaacgcat ataaaaagta cttttcaatt attggatgga tagtgtttac
     3241 attaggattg ttatgcattc cttttttgag atttttagtt aaaggttcga ttcctggaaa
     3301 tttaaatatg acagttttgt atttaatgta tttgataaat actgttttgt cgtattggtt
     3361 attttcatat agaaagagtc taatatatgc acatcaaaga aatgatgtaa ttaacgtgtc
     3421 tatgctaatt acttttacat tccaatttgt aatgcaggca tatgttctga ttttcttaaa
     3481 aaattactat ttgtatttat tggcttctat tttgggacaa atagttctta acttaatgtg
     3541 tgctgttgtt tctcaaaaga tgtatcctga ttatactcct gttggtgagt tagagaaaga
     3601 aactattgac gatattataa aaaaagtaaa aggattagtt acaaacaagg taggcggtac
     3661 aatattacga tcttcagatt caattattat ttcatctttc ttaggtcttt cagtacttgc
     3721 gatttatcag aattattact ttattatttc tgccataatg agtatatttg caataattta
     3781 tgaatcatgt atagcaggta taggtaacag tcttattgta gaaagtcaag aaaaaaatta
     3841 tatagatttt tgtgatatta caacaattat aggttggttt ctggcaatta gttgtgcatg
     3901 ctttattaca ttatttcagc cgttcatgat tatctgggtg ggaagtgata atcttatgag
     3961 tatttcattt gttatattta tgatagttca ttttttctta tatgaaattg atcagttaat
     4021 tgggttatac aaagatgctg ctggaatatg gtacgcagac aggtttagac cattaattac
     4081 agcaatgata aacattgtat taaatatcat cctcgttaat tggttcggat tatatggagt
     4141 tttggcggca accattgttt cagtattgtt ggttgattta ccctggttat tatataatgt
     4201 tttccaaaat atatttaaat ttgaatcggc aggtaaatat gtttggaagc tcataagttt
     4261 gtgtgtaata gcttttgttg cttcttattt aagttatttt gcttgttcgt atgttaatat
     4321 cggaaatttc aaagtgataa gtttagtgat aaatttagtt atatcggttg tcatatcgat
     4381 tttagttatt cttctgttta atatccgtaa taagaagttt aagtatattt tgaaagttct
     4441 tacgtcggta ttggtaaaaa gatgagaaga gacatcagaa tgatatataa agtaactaca
     4501 atttttaggc tgccgaatag gcgaacgatg tatagttttt gttccaacga agactcttgt
     4561 tgaaattgga gatgatatga aggagttaca attttgactc atgtttatga ttgatcggtt
     4621 ttaaaagtag tatggagaag ctttgggatc atcggagagg atcaaaatag gtaataatgt
     4681 ttttatagga atgaagtcta gaatactaaa gggtgtacat ataggaaata atgtagttat
     4741 aggtgcaaat agtttagtaa acaaggacgt atcagacgat tgtgttgttg caggaaatct
     4801 agcaaaagta attatgtccc tagatgaata ttataaaaag ataaacaata tgaagaatcc
     4861 gtaaaacttg tgaaactata ccgagaataa tatgggaaaa atattgatga aaaagcatta
     4921 tatgagtttt tagggctgtt tacagatgga gatgacaatt tgtcgaagag ttgggagcaa
     4981 tgcaaaattt agtaggggat tataaacaga ctgacaaggt atttaaagaa catgcacaag
     5041 tatttcataa tattgaagaa tttttgaata ctgtgagata ggagttgcta cattgagaaa
     5101 agtatgtatt tttccaatgt aaaagattac gagtttgaaa acgacaccat aatattattt
     5161 tttaagaaga tattatagag cttagg
//
LOCUS       DAZKCC010000128         5125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000128 DAZKCC010000000
VERSION     DAZKCC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.132"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1341)
                     /locus_tag="ACG8VH_002812"
     CDS             complement(67..1341)
                     /locus_tag="ACG8VH_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFP9111300.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1749..2453)
                     /locus_tag="ACG8VH_002813"
     CDS             complement(1749..2453)
                     /locus_tag="ACG8VH_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111301.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(2640..2885)
                     /locus_tag="ACG8VH_002814"
     CDS             complement(2640..2885)
                     /locus_tag="ACG8VH_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111302.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(2902..3753)
                     /locus_tag="ACG8VH_002815"
     CDS             complement(2902..3753)
                     /locus_tag="ACG8VH_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9111303.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            3919..4923
                     /locus_tag="ACG8VH_002816"
     CDS             3919..4923
                     /locus_tag="ACG8VH_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP9111304.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1556 a          911 c         1014 g         1644 t
ORIGIN      
        1 tggcgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg tgcgccgcat
       61 atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc gaaagttata
      121 aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat tcagcccctc
      181 agtgacgccg tttgagtggg gcaccttaaa ggcatttgaa atacccgatt ggtagcgatg
      241 gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg ttttaagggt
      301 ttcttgaaag ccttccgtat ctcgtttaac gaaataatag cgtaataatt gacaaaaatg
      361 atacgcagtt cgcaattctt cgttgtagct taacagctca tcaacgattt ctgtttgact
      421 aattggtcgt ttaaacagtc gatgataatg ccgtttgagt ggctccaatg tcgtggaatc
      481 tttgagtaag agtttccaat atcgcttgag ccgacggtac ttcttctgat tttcagagtg
      541 tgaggttcga aaacgattca tgatctggac acgtaattga ttgaagctgc gattgatgtg
      601 ttggacaata tgaaagcggt cagttacgat ctccgcattt ggaaagactg ttttaagcaa
      661 ctgacaatag ctggcattca tgtccatcac taggtacttg acctttaaac gggcctttct
      721 aggaaagcgc aggaagtatt gagttagttt gtaaagtctg cgatcttcaa ggatcgtaaa
      781 tagctgattg gttgtcccat caacacaaat aaaactcatg gaaccagaaa cagacttcat
      841 tgatttgaac tcatccatac acaacaccgt cggaaggaag ttcatacgtg gctggtaatg
      901 cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc gagacgaagt acttcttagc
      961 gattagttta cgtgattgat tttcgtacag ttccatgaga atcgcctgct tcagttcctt
     1021 tgttagttga tgatgttcat caactaagtc cgttttcgca gaaaaggtcc gtccacaatc
     1081 atggcagaga aaacgggtac gttttaattc taaaatagtc aacttttctt tgactttcaa
     1141 catctgagtg cgtgtcgtat aggaaccgtt gcggataata cgaatcgatc cacagtgttc
     1201 acaatgggaa ggtgagtcat ccaatctccc tttaataaac tgcgcttgaa cgcctttata
     1261 ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta atccgagtaa
     1321 ttttctagta tgattatcca ttgagagact cctttggtag ggtgacgttt tggtcgacta
     1381 aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaaagtg ttagtagaaa
     1441 atcgctttcg attttccacc aacacaaaat attatagaac cgcttttttc tctatcagaa
     1501 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     1561 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     1621 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     1681 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     1741 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     1801 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
     1861 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
     1921 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
     1981 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
     2041 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
     2101 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
     2161 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
     2221 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
     2281 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
     2341 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
     2401 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
     2461 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
     2521 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
     2581 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
     2641 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
     2701 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
     2761 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
     2821 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
     2881 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
     2941 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
     3001 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
     3061 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
     3121 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
     3181 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
     3241 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
     3301 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
     3361 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
     3421 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
     3481 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
     3541 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
     3601 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
     3661 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
     3721 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
     3781 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
     3841 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
     3901 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
     3961 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
     4021 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
     4081 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
     4141 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
     4201 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
     4261 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
     4321 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
     4381 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
     4441 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
     4501 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
     4561 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
     4621 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
     4681 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
     4741 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
     4801 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
     4861 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
     4921 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
     4981 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
     5041 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
     5101 ttcttggagt taaacgcttc tgtcc
//
LOCUS       DAZKCC010000129         5113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000129 DAZKCC010000000
VERSION     DAZKCC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.133"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..399)
                     /locus_tag="ACG8VH_002817"
     CDS             complement(61..399)
                     /locus_tag="ACG8VH_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFP9111305.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(415..618)
                     /locus_tag="ACG8VH_002818"
     CDS             complement(415..618)
                     /locus_tag="ACG8VH_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111306.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(633..1409)
                     /locus_tag="ACG8VH_002819"
     CDS             complement(633..1409)
                     /locus_tag="ACG8VH_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9111307.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1708..2028
                     /locus_tag="ACG8VH_002820"
     CDS             1708..2028
                     /locus_tag="ACG8VH_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111308.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2046..2477
                     /locus_tag="ACG8VH_002821"
     CDS             2046..2477
                     /locus_tag="ACG8VH_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9111309.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            2607..3068
                     /locus_tag="ACG8VH_002822"
     CDS             2607..3068
                     /locus_tag="ACG8VH_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFP9111310.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            3102..3737
                     /locus_tag="ACG8VH_002823"
     CDS             3102..3737
                     /locus_tag="ACG8VH_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFP9111311.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            3850..4485
                     /locus_tag="ACG8VH_002824"
     CDS             3850..4485
                     /locus_tag="ACG8VH_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111312.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(4478..4612)
                     /locus_tag="ACG8VH_002825"
     CDS             complement(4478..4612)
                     /locus_tag="ACG8VH_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111313.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            4754..>5113
                     /locus_tag="ACG8VH_002826"
     CDS             4754..>5113
                     /locus_tag="ACG8VH_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFP9111314.1"
                     /translation="MYQRKRADSIMARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKT
                     KGEAKDAANELEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMN
                     IKKYMPAVLISDLDRVGY"
BASE COUNT         1814 a          804 c          836 g         1659 t
ORIGIN      
        1 ctgcttcccc aaagtcctaa agcgtagatg attagcggtg cgctgaacac tagcgctcta
       61 ttaaattttc ccatccgctt gcctccttaa gatttccgaa aaatttgttt ctaaaaattc
      121 aagtgtttta cttcttaaaa atagatatgt gtctcttcct tcaactggat aatagacaaa
      181 tccatttttg tttttctcga tatcgataat atttctatat cttgggttct ttaaaactct
      241 agaagtaaac caatcatatt ttctgttaat ccgttctagc acttctggca acgtcatcca
      301 tctaccagta tcatcagctt tttttaactc ctcataatcc acttgggaga taattacata
      361 gccttctgga attgggattt ttgcttctag atattgcatt agctgttcct ccctttacaa
      421 ttcgtacata gtgataatcg aatctataat tctgtttgct tctgcagaag tctttttacc
      481 gtttaaaatt aaagataagt agcttttgct aattccaaat ctttcagcaa gcatggtgta
      541 agttaagaac tttgaacttt cgacatattc tttgattttt tctctatctc gttgagtgat
      601 ttctgcaatg tcagtcatac taaaactcct ttctaaccaa tttcctctaa atccatttga
      661 gggtaatatc cttctttttt tagtaattcg tagataaata gacgcccttt ctgtgtccat
      721 ttggtattca ttacaatttt agtgccacca tcggctttcg ggatctcagt tgtatgagat
      781 tttgtgtatc cttggttcat gtgttttttg cacaataacc attggttacc gacttttttc
      841 tgaataccta gtttatgaag taatttattc atctgttgtg gagacatccc ataatctgct
      901 gcaatctgac taattgttac tgaatctgta gaagataata tgctatctaa ataggagatt
      961 ttaggttcgt actctgcaat cttttgttct gcgattaatc ttccagttct ttcttctttt
     1021 agttgagttg ctaattgaat gattgtatct ggattaagca aagcttcttc tactttttct
     1081 ggagttagat aacctccatg ttttctaatt gctggcaaca cttcacttgt tacccaacgt
     1141 ttaaattttt tggcagaggg aagttttgat tttaagatta aactgtacaa accagattcg
     1201 ttgatgattg tcatctctct tgattgacct gaggtcgtga ttcgcgaccc catcttatct
     1261 tccaaatcta catgccgcga caaagcatct ttagtgtttg aataacccaa aacactcgca
     1321 acgtctttgc ctacaaaata tggttcatca tttactaaaa ttgtccgaac ttcgttttgt
     1381 tcgaaattaa aaatttgcgg tgtgttcata tttctcattc ctttctttta tattcgtaaa
     1441 caaatttaac aacttttttc taaattcaat tgacagtatc tgagtaatac tctatactaa
     1501 gagcataatt aaataagaca taaaaacatt gattttaaag ctttcttggc ggtctgcatt
     1561 tacatatcaa tagtgttttt tgttgtcttt ttagttgtta aatctgttta caagatatag
     1621 tatagctcat tactcagaat tagtcaaatt atttctgcgt atttttctaa actttttttg
     1681 taaacattta gaaaggttgt tttatcgatg aatacctatg aaataataaa agacttggcg
     1741 aagaggaaaa agatatctat acggcagtta gaaattaatt ttggatattc taacggttat
     1801 ctagcaaagt ggaaaaataa tactcctaat tcagatgaat taagtcggat tgctgactat
     1861 ttcgatgtct ctgtagacta ccttctaggt agagaagaaa gagaaacccc taaacatgtg
     1921 gatttatcag aagacgatac tgttttttct tttgatggaa aagaaatatc taaggagaca
     1981 atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg
     2041 tttgtatgta tttgaaattg aaagaaatgt tgagcgaata caatttgaaa gtagtttata
     2101 tggagatgaa agagcctggt ttctactatc caaaacccag aatcattttt ttgaatgaaa
     2161 atctatacgg tgaaactgca gaagcctttc atttatctca tgaacttgca catttcagcg
     2221 cctctcattt tgaattttca gttttgtacg atacttctac aacttttcac tcaaaatttg
     2281 aaacagaagc agataaaata gctattctaa ttttattaaa tatctatatt gagaacgaac
     2341 tgactgatga atctcagttt aatctcgaaa aatttatgga gtattattct attcagaata
     2401 agctcagata cacttgttat gctgtctgcc aatgctattt taagaaaaaa tattcttacg
     2461 caaggcaata tgtatgaata catcaagaat tattaaaaaa accgatttca gcaaaacatt
     2521 aaaaaagccc gtgctgcaac acgggctctt tcctcatata tgagcttcta caaaaaaaat
     2581 catatcacag aaatgaggaa gaaaaaatgg aaaaagagat tttaataaaa tctccaggaa
     2641 aaaccttgat taaagtaact gatgattcta tttcaattat tagaaaagga tttataaatt
     2701 tagttaatca aggtattaaa ggagaaaaaa ctatcccctt taaaaatata tcagccgtac
     2761 aattaaaaaa accaggtatg agtaatggtt atatacaatt taccttgcta ggcggaaacg
     2821 aaagtcgcgg aggtatatta gcggccacga aagatgaaaa caccgttatg tttacaaaaa
     2881 aatattggaa tgaaatggaa aatttaaaaa aatatataga gaagcaacaa tctatattag
     2941 ataataattc caaagaaaca caaatttcaa gtgcagatga aattaaaaag tataaagagt
     3001 tgttagatga agggattatt aatcaagaag aattcgattt taagaaaaaa gaattgcttg
     3061 gtttataata ttcaatatca aaagagaaag aggatctatt aatggacggt atatttggtt
     3121 ttactggtat tgttttattt attataggtt taatcatgtt aattgtaaga tttatcaaaa
     3181 aaacaaataa gaaaacacct atgattctcc ttattatcgg gattattttt actaccgtag
     3241 gtttctcgct ttcctcatca aacgaggcaa atgacaataa caaacaggaa agctcatcat
     3301 ctgatactca aactactaca tctaacagca gcaataaaag cctaactcaa tttgaagaat
     3361 ttttagagac aaataatcat gactggggtg ctttcttaga tagttattat tctattactc
     3421 cagctactga gcaaaataca gcttttacta aatatattag tggtaaaaca tatacttttg
     3481 aaggaactgt tatcgaatca atgactacta gaatagccat tatagcagac aaagagtacg
     3541 atgataaaag ttggaatgat atttctgcaa cacctaaagt ttcatatgta atttttgcga
     3601 aggacttaaa caatgctgat acctttacaa agggcgataa agtaactttt acaggagaaa
     3661 ttagttcaag aggatcgaac atagaaaaat cctatgctca atgggatatg attaatagta
     3721 aagtaagtaa aaaataaatg aaccacgccc cactaagagt tagattgtgc acaataataa
     3781 aacctataca aaagagtgcc atcaggagtc acaatgcttg taaaggaaat gacaaaatag
     3841 gaggtaacta tggataatag caatgaaaaa ctaaccaaaa aagaatatct tgaattaata
     3901 gttgaaggcg gtattggagc aattccttat attggtggcg ttgtgcagtc actttatttt
     3961 gggtctaaaa acgagaagcg tttcaaacgc atcgagaaat tctacaaaaa tttaaacgaa
     4021 tcattagaaa acattcgcga tcaaattcca gaagatgttt tcaacttaga gaacaaggac
     4081 caactcattg gaatattcga agggataaat gacgaggtag aaaaatcaaa ggcacaaaat
     4141 aaaatagata tgtataaaaa tctttataag aattgtttac ttcgaattaa caacgcttca
     4201 tgggataacg aggagtatta tcttcttgta ctgaatcaat taacatcgat tgagattcaa
     4261 ttgttagcat gcttaatgag tcgtggaaac gaaaatttca ctggaaatat cagcatgtct
     4321 ggctattcac aagagctaat agatggtagc ttaaatagat tatctgattt tggtctgtta
     4381 gaaaaaacga ttagctccat agtattaggt gatgtcggca aacaaaatat gggctacaaa
     4441 atatctcatt taggaattca atttattaac tcaactctta cttaagtttt ctttctaata
     4501 tgacatctag acctatcagc aatgcaacaa taaaaaacca gacataccaa taatcaacaa
     4561 ataatctaaa ctctggaagt tcagttattc ttttaaaaat cactttaatc ataataaaca
     4621 cctctgattg gatgatctaa tacacatcca attataccat taaaacgaca aatagcacat
     4681 ccttgcgccg gcaagcaaaa aggatgtgca aaaaataata aaacccaaac aatgggcttc
     4741 tttatagttc ctattgtatc agagaaagag agctgattca attatggcaa gattagtcaa
     4801 acgtggaaat agttggcaat acgaaatttc atacaaaaaa gatgacggaa aatacacgaa
     4861 gataagaaaa tcaggattta agacaaaagg agaagcaaaa gatgccgcca acgaattaga
     4921 atataacttg aacaaaggcc ttaaagggga tcgcaaaaat ctattattat cagattactt
     4981 tgaggattgg atgcaacttt ataaagaagg aacagtatct cctatcactt atagaaaata
     5041 cgaagatacg ttaatgaaca taaagaaata tatgccagcg gtattgattt ctgatttaga
     5101 tagagtggga tat
//
LOCUS       DAZKCC010000130         5007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000130 DAZKCC010000000
VERSION     DAZKCC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.135"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8VH_002827"
     CDS             complement(<1..182)
                     /locus_tag="ACG8VH_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9111315.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8VH_002828"
     CDS             517..951
                     /locus_tag="ACG8VH_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9111316.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8VH_002829"
     CDS             974..1294
                     /locus_tag="ACG8VH_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111317.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8VH_002830"
     CDS             1435..1626
                     /locus_tag="ACG8VH_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111318.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8VH_002831"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8VH_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP9111319.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8VH_002832"
     CDS             2112..2375
                     /locus_tag="ACG8VH_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111320.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8VH_002833"
     CDS             2399..2587
                     /locus_tag="ACG8VH_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111321.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8VH_002834"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8VH_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111322.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8VH_002835"
     CDS             3070..3441
                     /locus_tag="ACG8VH_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9111323.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8VH_002836"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8VH_002836"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8VH_002837"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8VH_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111324.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8VH_002838"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8VH_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9111325.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1744 a          784 c          978 g         1501 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt attttta
//
LOCUS       DAZKCC010000131         4947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000131 DAZKCC010000000
VERSION     DAZKCC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.136"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1771
                     /locus_tag="ACG8VH_002839"
     CDS             128..1771
                     /locus_tag="ACG8VH_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9111326.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(1844..2389)
                     /locus_tag="ACG8VH_002840"
     CDS             complement(1844..2389)
                     /locus_tag="ACG8VH_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP9111327.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2412..2897)
                     /locus_tag="ACG8VH_002841"
     CDS             complement(2412..2897)
                     /locus_tag="ACG8VH_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9111328.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(2887..3699)
                     /locus_tag="ACG8VH_002842"
     CDS             complement(2887..3699)
                     /locus_tag="ACG8VH_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP9111329.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(3838..>4947)
                     /locus_tag="ACG8VH_002843"
     CDS             complement(3838..>4947)
                     /locus_tag="ACG8VH_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP9111330.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         1593 a          977 c          946 g         1431 t
ORIGIN      
        1 aaaataaagc tcaacgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg
       61 gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg
      121 aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc
      181 tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa
      241 acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc
      301 tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg
      361 aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat
      421 tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa
      481 atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga
      541 accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg
      601 acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa
      661 attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga tagatggatt
      721 tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa
      781 ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca
      841 tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga
      901 aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat
      961 ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga aacttgcatt
     1021 aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca
     1081 tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc
     1141 tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg
     1201 acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga
     1261 ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga
     1321 tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca
     1381 atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat
     1441 caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat
     1501 ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga
     1561 acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt
     1621 aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac
     1681 attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta
     1741 tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga
     1801 ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag
     1861 gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt
     1921 tcaaacgagg tctgacaggt acttggacat gtttgatatg cataccgatt gatgtgtcac
     1981 cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt
     2041 caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt
     2101 cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac
     2161 aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga
     2221 tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg
     2281 tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat
     2341 taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc
     2401 ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga
     2461 aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca
     2521 gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt
     2581 cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca
     2641 tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc
     2701 aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga
     2761 aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca
     2821 gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt
     2881 aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc
     2941 ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc
     3001 tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa
     3061 ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata
     3121 atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt
     3181 gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt
     3241 taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat
     3301 catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac
     3361 agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt
     3421 ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat
     3481 accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga
     3541 tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct
     3601 tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc
     3661 aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa
     3721 gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca
     3781 atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta
     3841 ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt
     3901 tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac
     3961 ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg
     4021 tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa
     4081 taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc
     4141 tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc
     4201 gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt
     4261 agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt
     4321 aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt
     4381 caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac
     4441 ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt
     4501 gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc
     4561 cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg
     4621 gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg
     4681 aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat
     4741 gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt
     4801 ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga
     4861 agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc
     4921 aggttgttcc attaaataaa gaatagg
//
LOCUS       DAZKCC010000132         4929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000132 DAZKCC010000000
VERSION     DAZKCC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.137"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(324..686)
                     /locus_tag="ACG8VH_002844"
     CDS             complement(324..686)
                     /locus_tag="ACG8VH_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111331.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(760..1140)
                     /locus_tag="ACG8VH_002845"
     CDS             complement(760..1140)
                     /locus_tag="ACG8VH_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP9111332.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1222..2397
                     /locus_tag="ACG8VH_002846"
     CDS             1222..2397
                     /locus_tag="ACG8VH_002846"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9111333.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2474..3058)
                     /locus_tag="ACG8VH_002847"
     CDS             complement(2474..3058)
                     /locus_tag="ACG8VH_002847"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9111334.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3259..4257
                     /locus_tag="ACG8VH_002848"
     CDS             3259..4257
                     /locus_tag="ACG8VH_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9111335.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4369..4878
                     /locus_tag="ACG8VH_002849"
     CDS             4369..4878
                     /locus_tag="ACG8VH_002849"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9111336.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1565 a          920 c          855 g         1589 t
ORIGIN      
        1 ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt ttagaaatgt
       61 gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc ttccttggtc
      121 aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta ttattcgtta
      181 tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc tttagcagaa
      241 catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact
      301 tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc gtaagttcca
      361 agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa ttcttctctt
      421 tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt aatatgtgta
      481 tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc ttttaagaaa
      541 ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc aaattccaac
      601 ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg caacataatt
      661 gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt ctatggctta
      721 cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat tttctttccg
      781 ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa tagtggtaaa
      841 tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa cagctggatt
      901 tgttttttct aacgttctta aagataaact gatttttttg gaatattcat caatgtcgat
      961 gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt tcgtataacc
     1021 ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc
     1081 gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt tataagtcat
     1141 ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa agaagcgacg
     1201 aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag aaaagataca
     1261 gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt acttcctttg
     1321 tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac
     1381 atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca ggctatcatt
     1441 cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt
     1501 ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg cgattctatt
     1561 ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa tcaacgtcac
     1621 atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt gtctgacatg
     1681 gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct
     1741 ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg tttaaaacat
     1801 caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga tcatacattt
     1861 acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt tacttccgca
     1921 accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa aaatccagag
     1981 atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat caacactttt
     2041 ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag ccaactgatt
     2101 ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa
     2161 gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac cgcctatatt
     2221 tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt attaaatcct
     2281 ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc ttgtccagaa
     2341 gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat cgattaaatc
     2401 atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca taattactcc
     2461 gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca tgtacaggtc
     2521 ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc ccttctagta
     2581 catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct ttatacgctt
     2641 caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta gctggcacat
     2701 ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa tttgccatag
     2761 ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa ctttcgccat
     2821 aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc atgaaatcag
     2881 gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt tcaatgaaat
     2941 tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca ccacggtttg
     3001 ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg aatgccatat
     3061 tgtttcctcc taatatttgc atacttcata ataataataa cataatacct aacctctcgt
     3121 aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta taacttaccg
     3181 tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct gctatagtgt
     3241 gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc aggtcctgta
     3301 gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat cattgacagt
     3361 ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat ttacgatatt
     3421 cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa acaattaact
     3481 acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg tgaagacgaa
     3541 gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat tttagcttta
     3601 ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc atttgaaaac
     3661 cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt
     3721 gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat tgcaagcgaa
     3781 gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt atcacgcttg
     3841 aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc aggcaataat
     3901 ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg
     3961 attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct ttcttcttgg
     4021 ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac ttctattcca
     4081 ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact gattgctact
     4141 gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa tccaaaggaa
     4201 cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc tagttgattt
     4261 tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt
     4321 tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa
     4381 actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa
     4441 gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt gactttggcg
     4501 gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa cgccattatc
     4561 actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc gttgcaacat
     4621 attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt gtcaatcgtg
     4681 aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt gaaacctggt
     4741 attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt agatgatatc
     4801 gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc agaaaaaaga
     4861 agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta gacgagttct
     4921 ttctttttt
//
LOCUS       DAZKCC010000133         4823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000133 DAZKCC010000000
VERSION     DAZKCC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.138"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8VH_002850"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8VH_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFP9111337.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACG8VH_002851"
     CDS             complement(2944..3378)
                     /locus_tag="ACG8VH_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111338.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="ACG8VH_002852"
     CDS             complement(3395..4600)
                     /locus_tag="ACG8VH_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP9111339.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT         1515 a          889 c          645 g         1774 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tat
//
LOCUS       DAZKCC010000134         4755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000134 DAZKCC010000000
VERSION     DAZKCC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.139"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..744
                     /gene="vanR-A"
                     /locus_tag="ACG8VH_002853"
     CDS             49..744
                     /gene="vanR-A"
                     /locus_tag="ACG8VH_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9111340.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            722..1876
                     /locus_tag="ACG8VH_002854"
     CDS             722..1876
                     /locus_tag="ACG8VH_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9111341.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2091..3059
                     /locus_tag="ACG8VH_002855"
     CDS             2091..3059
                     /locus_tag="ACG8VH_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9111342.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3052..4083
                     /gene="vanA"
                     /locus_tag="ACG8VH_002856"
     CDS             3052..4083
                     /gene="vanA"
                     /locus_tag="ACG8VH_002856"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9111343.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4089..4697
                     /locus_tag="ACG8VH_002857"
     CDS             4089..4697
                     /locus_tag="ACG8VH_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9111344.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1470 a          910 c         1115 g         1260 t
ORIGIN      
        1 taatctgata aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa
       61 atacttattg tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac
      121 gagaattata cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag
      181 tctgagattg accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc
      241 tgtcaaaaaa taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca
      301 gaggtagata aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt
      361 cgcccactgg agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt
      421 ggagtaaagg agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac
      481 acccatgagt gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata
      541 ctgcgaatcc tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag
      601 atatggggcg acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat
      661 ttgcgcgaaa aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg
      721 gttggttata aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta
      781 catgtatatc gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat
      841 gatccgaggg aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa
      901 tcacctggac gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat
      961 ttatgtggcg attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc
     1021 aaaatacttt gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca
     1081 aattgagctt tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac
     1141 tctggaaaag cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat
     1201 gtacttggcg cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct
     1261 tgacgaggct ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga
     1321 caaagcgtat cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct
     1381 acaaacgata acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac
     1441 cgatgaattt tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga
     1501 ggatctgacc gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa
     1561 aaacgccgct gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg
     1621 ggatgtggtg tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc
     1681 catatttgaa aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg
     1741 acttggattg gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga
     1801 aagcaatgat aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga
     1861 taaaaggagg tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac
     1921 tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact
     1981 taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt
     2041 ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca
     2101 tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc
     2161 ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat
     2221 ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc
     2281 ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc
     2341 atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc
     2401 cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat
     2461 cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac
     2521 tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc
     2581 ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa
     2641 actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc
     2701 tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag
     2761 catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat
     2821 tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt
     2881 tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa
     2941 tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata
     3001 ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga
     3061 ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct
     3121 gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt
     3181 acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat
     3241 tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac
     3301 catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt
     3361 gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat
     3421 attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct
     3481 gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg
     3541 tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa
     3601 gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa
     3661 atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt
     3721 gccgcgttag ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt
     3781 catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac
     3841 ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc
     3901 ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg
     3961 aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc
     4021 gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg
     4081 tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg
     4141 acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa
     4201 atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg
     4261 ctacccaagg gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact
     4321 gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca
     4381 atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc
     4441 gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg
     4501 ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca
     4561 atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat
     4621 atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg
     4681 atttccccgt taaataaact tttaaccgtt gcacggacaa acggttctgt tgcaaagttt
     4741 taaataaaga ataaa
//
LOCUS       DAZKCC010000135         4691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000135 DAZKCC010000000
VERSION     DAZKCC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.140"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG8VH_002858"
     CDS             complement(136..1098)
                     /locus_tag="ACG8VH_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9111345.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG8VH_002859"
     CDS             complement(1100..2539)
                     /locus_tag="ACG8VH_002859"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9111346.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG8VH_002860"
     CDS             2724..4670
                     /locus_tag="ACG8VH_002860"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP9111347.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1485 a          807 c          774 g         1625 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag a
//
LOCUS       DAZKCC010000136         4484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000136 DAZKCC010000000
VERSION     DAZKCC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.141"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1007
                     /locus_tag="ACG8VH_002861"
     CDS             279..1007
                     /locus_tag="ACG8VH_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9111348.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1152..3179
                     /locus_tag="ACG8VH_002862"
     CDS             1152..3179
                     /locus_tag="ACG8VH_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP9111349.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3287..4384
                     /locus_tag="ACG8VH_002863"
     CDS             3287..4384
                     /locus_tag="ACG8VH_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9111350.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          725 c          922 g         1263 t
ORIGIN      
        1 ttttacactc tcgtcaagtc acattttgat agataggagg gggacttacg aattatttta
       61 aaggctatga cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa
      121 atcaaatcgg gctgataatc atttataact aatcgataca taggaccaaa tataggtcta
      181 tgttctttta tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac
      241 tgatatgttt agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa
      301 cagcagcaat gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag
      361 tatacccaaa ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg
      421 taactcctcc tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc
      481 ctccaagccc tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg
      541 ggactcaaaa aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg
      601 atgggtctag aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt
      661 ggaaattaag tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg
      721 gagcacagct gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc
      781 cgcaaacggt gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta
      841 atgctaaatc aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg
      901 aaaatcaaga tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat
      961 attccactaa attagtttgg acattagaag atacacctaa caactagttt actgaaaaga
     1021 actgttaaaa ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca
     1081 aaggctaggc taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag
     1141 gaaaagatat attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta
     1201 atatctttcc gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa
     1261 gagacgaaga aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac
     1321 aagaaagcag tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag
     1381 ctgagggttc ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg
     1441 acgatcccaa tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt
     1501 ccatattgga aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag
     1561 ctatttggtc aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat
     1621 acttaggaaa tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc
     1681 ctagaatggc tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat
     1741 attctactcg ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt
     1801 ataaaaatag tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc
     1861 atggacatgt cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata
     1921 ctggcgtgac ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta
     1981 aatgggacgc ggagcaaaga agtcttactt atgatttgga aggtgtcggt cgctcttctc
     2041 ggattatacc tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt
     2101 cttcaacagg tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg
     2161 tcacatcaac agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg
     2221 gaggaaacga taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat
     2281 ataagaaaat gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct
     2341 tgatcaatgg aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc
     2401 taaatctgac agattatttt attgctggag atacgttaat aatcgaattg gagacaactt
     2461 tgcaggaagt aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga
     2521 atttgattga gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata
     2581 tccatgttta ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca
     2641 ctgggaaaat aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg
     2701 actcggattc agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt
     2761 attacagaaa aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac
     2821 ataaaatatc cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta
     2881 tagatgaacg ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa
     2941 atggagattt tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta
     3001 tagggacatc cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct
     3061 ctcttttaga ttctacagct catcgtggaa tcagaataaa tattcctgta gaaaaccaaa
     3121 gaatcggaac ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac
     3181 aggaagagaa gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct
     3241 cttgtgtccc acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc
     3301 gattttcttg agaccaaaac aacaattatt tataacactc ctagttattt taggggcact
     3361 ttttaacaat atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc
     3421 agagaatcaa atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga
     3481 agtagaagta aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc
     3541 tgtgaatagc gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga
     3601 aaaagacatg agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac
     3661 gctaaaacct aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt
     3721 tgatggtgta ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac
     3781 agaaactaag gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt
     3841 gatgcaacag acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga
     3901 tcaaatcaat gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat
     3961 caatcaagta gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca
     4021 agaggaaaaa gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt
     4081 aaatggtcaa ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga
     4141 aaatgaaaat ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca
     4201 atgggtattt gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac
     4261 ggatgttacg attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt
     4321 attgtttatt ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa
     4381 gtaaggttaa gataacaaat agtaaatttt tattattgta ggatggttgg gctcttttta
     4441 taacaatata aaatatacca ttttattaat aaaaatgcat acta
//
LOCUS       DAZKCC010000137         4377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000137 DAZKCC010000000
VERSION     DAZKCC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.142"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8VH_002864"
     CDS             73..234
                     /locus_tag="ACG8VH_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9111351.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8VH_002865"
     CDS             253..1530
                     /locus_tag="ACG8VH_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9111352.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG8VH_002866"
     CDS             1540..2196
                     /locus_tag="ACG8VH_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9111353.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG8VH_002867"
     CDS             2196..3572
                     /locus_tag="ACG8VH_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9111354.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACG8VH_002868"
     CDS             3702..4115
                     /locus_tag="ACG8VH_002868"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111355.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACG8VH_002869"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACG8VH_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKCC010000138         4304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000138 DAZKCC010000000
VERSION     DAZKCC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.143"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8VH_002870"
     CDS             complement(<1..149)
                     /locus_tag="ACG8VH_002870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9111356.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8VH_002871"
     CDS             complement(593..1714)
                     /locus_tag="ACG8VH_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9111357.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8VH_002872"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8VH_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFP9111358.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8VH_002873"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8VH_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFP9111359.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
BASE COUNT         1419 a          774 c          812 g         1299 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatt
//
LOCUS       DAZKCC010000139         4295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000139 DAZKCC010000000
VERSION     DAZKCC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.144"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..790
                     /locus_tag="ACG8VH_002874"
     CDS             <1..790
                     /locus_tag="ACG8VH_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9111360.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="ACG8VH_002875"
     CDS             887..3136
                     /locus_tag="ACG8VH_002875"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9111361.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..>4295
                     /locus_tag="ACG8VH_002876"
     CDS             3139..>4295
                     /locus_tag="ACG8VH_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9111362.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1501 a          610 c          791 g         1393 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact agagt
//
LOCUS       DAZKCC010000140         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000140 DAZKCC010000000
VERSION     DAZKCC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.145"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8VH_002877"
     CDS             complement(37..1257)
                     /locus_tag="ACG8VH_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9111363.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8VH_002878"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8VH_002878"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9111364.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8VH_002879"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8VH_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9111365.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8VH_002880"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8VH_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111366.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKCC010000141         4230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000141 DAZKCC010000000
VERSION     DAZKCC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.147"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..467
                     /locus_tag="ACG8VH_002881"
     CDS             30..467
                     /locus_tag="ACG8VH_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111367.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            562..1446
                     /locus_tag="ACG8VH_002882"
     CDS             562..1446
                     /locus_tag="ACG8VH_002882"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9111368.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1668..>4230
                     /locus_tag="ACG8VH_002883"
     CDS             1668..>4230
                     /locus_tag="ACG8VH_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9111369.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1380 a          711 c          957 g         1182 t
ORIGIN      
        1 tattaaaaaa tgcacgtaga agtagataaa tggagtggtt ttgcaaattt cttttatttc
       61 tagaagttat tcacgtatac ggatttgttt ttctcacaaa taaaaaggca ggaagtacaa
      121 tggatcattt gtatataaaa tatattcatc aattttccgt tttggtatta ggcacactaa
      181 ttatagcggg gattgttgca tttattattc aaggtcgtga catattaaca gtacatcaag
      241 agtttttcag ttatgcattt ggtattcctc tgatcgctat aaggtctgat aacaaaaagt
      301 ggaagaaacg ctattacatt ctcttgctat ttttgtgttt ttatctgcca cctaagattt
      361 ttactttatt aggtatacaa tgcctatcat tttcttttat ttcctatatc attgttttaa
      421 ttttcctcag tttcatagta tgggtatttt atcttgtcaa aaaataaatg aatgtttcat
      481 gaacagtact tcatcacagc ctaatttttt tctttatgct atcgttatat taaagaaatc
      541 aatcagaagg gaaagaaagt gatgaaaaaa gttttattag tggtaaaccc cagttcaggc
      601 ggtgaacaag caaaggaatt tgagcaatta gcgatagcaa aattggaatc ggtgtttgat
      661 gaagtggtcg tcttgcatac aaaaaaagca ggggatgcaa aaaactttac tcgcgaagcg
      721 gctacggaag ggtatcatag tgtgtttgtc atgggcggag atggaacagt caacgaagga
      781 atcagtggaa tcgctgaaca agaacatcgg ccaaatttcg gatttttccc attgggtacg
      841 gtgaatgact tggctcgagc attagggata ccgctagaac ctgaggaagc gatcaatcat
      901 ttttcgattg aatcagtcaa gccattggat attggaaaaa taaatgacga ctattttatg
      961 aatgtcgtcg caattggctc tatccctgaa gcaatcaatg atgtagatgc ggagaagaag
     1021 acgaaatttg gaaaactagc ttatttcatg tcaggaatca aacagttagc ttccacacaa
     1081 tcctactcgt ttcacgtaga agttgatgga aagaaggaag aaattgagag cagtatcttg
     1141 ctgataggac taactaactc agtaggcggt tttgaaacat tacttccaaa tgcaaaagtg
     1201 gatgatggta agctccattt ggtttattta aaagattctt ctttgatcga tacgcttaaa
     1261 acattgccag acctattaaa aggagtagat gaatctacga aaaatctagc ctatcttact
     1321 tgtgagcaat tagttgtgtc attagctgat gaggaagaat tgacgacgaa tgttgatgga
     1381 gatgaagggg ataaattacc tgtgacaatc aaagtgttac cctcacattt acaggtttat
     1441 tgttaaaatg gaggttgtga ccgaagagtt gtcaggagga catcggttgt tcggaataag
     1501 ggactgtgac aagacttttg tcacagcctt tttttgtaca aaaaagaaat tttcacctaa
     1561 cagaaaaatt tgccataaaa atacatattt taaatatgaa agtacaatta caaataaatt
     1621 aaaacgttaa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatatg
     1681 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
     1741 ttgccgatgt agagaaaaca agcgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
     1801 ctactttttc agaagaatca attacttctg aaactacaga atcccaaagt gaggcgccta
     1861 cttctaatac aaggcaagaa gaaaataaga caagtgagac ctcagaagta gctgaacaaa
     1921 cgtctgatag cagtgaagag gtacaggtta gagttgtttc agcggatggg tacagcgaag
     1981 ctgccacaac agaagaactg acgcagcttc tcgcagacga atcagtagct aagattcgtt
     2041 taactcagcc tttgacttta gacagggaat tggagatcaa gcgagatatc gttatcgatt
     2101 ttggcggact tgctcataat ttcggcacac atcacatcta tatcaatgag acacctcagt
     2161 cgatcgagtt tcagaatttt aaaggaacgg cagctcatcc aggtgggttg ataccaaata
     2221 tcgatggaaa tgccattatc attgcttata tgtccgattt ttggggaaac agatatcatt
     2281 ttacgggaga aataaaattt accgggacac ttgacttata tgatggttct aaattaggcc
     2341 tgatttacgc ccctcgtgca actgtcacat tagatggtgt ttccggtgta ttggatgtcc
     2401 aaccagtgaa agaagggatg aatgccgctc cgggtaaagc ttatttttgt agaagctatc
     2461 agctaaatgt aatcaatggt agtcagcttt atggaccata tctaggaaaa ttctatggtt
     2521 ttctagattc aggtgatgag acgggcagtg gaaaaacagc accaggactc catatcttga
     2581 gtggttcaaa ggtctctctt gactatgatc gggcagatgg cgtcagcgat gaaggagagg
     2641 ctgtcgatac gctacccggg aatgtcacat ttaaagtcag tggctcagga agtgaatttt
     2701 cggtaaatac aaagatcaat attaccaacg acaacaatcg cggaattatc caaatgagag
     2761 gctcaggatc aagagtcgct gtgtcgaatg atgggaaaat cacaatcaat acagcgacta
     2821 ctggcggatt tcgcctgcaa ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa
     2881 tctcggtcaa aatggctgga gatggcgacc aacctggaaa caatgggatg cgttttgtag
     2941 ggaaaggact ttctttgatc gtagaaggag ccggatcagt catcgatatt gataagcaaa
     3001 gtggtcgttc tagcgcagtt cgttttgaga acggtacgca aaaactaaca gtaactaatg
     3061 gaggctcatt gaaggtaaga aatgcagggg atggcaaatc ttatgtgaac agactcaatc
     3121 aaggaaacca agcgatccaa ttttatgacg cttctggttt tctagaaagt ccagggtcag
     3181 ctgattttac ggttactgga gaaaaatcaa atatcgatat acgcgcagat tccggagcta
     3241 ccgtagatac aactggagat attgatttga actttatggc tggcgaaaaa acttatttag
     3301 tcatggtcgg gaaaactggg gaagataaga ctggcatcat cagtggggat gtgttgaatg
     3361 tagacttaga atctcctacc tatttcgatt tccaaaacaa acggatcgga agcgagacta
     3421 atggcatagg tggctgggta tttcaaggaa atggcgattc ttctttgaag atcgctcatt
     3481 cagaaattgc tttatggcga aaggcaggag taacgttcga taactttgaa aaagatccgg
     3541 attatttttc tgatatgaca gacttatcga tcaatggaac cgacttaggt aattttgtat
     3601 caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga atgaaaaatt
     3661 ataatcgaat cagtgccaat aaccagcttc cgatgattga atccttaaga gtaccaacga
     3721 atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa
     3781 gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttatgca gatacgacga
     3841 aatcacctgt agaaattact gctctcacac aaggacaaac agatgaaaat gaagggacag
     3901 gatataatcc gtggggagaa ggcgaacaag gcggtttgtt tgaggttcaa gtacccaacg
     3961 aagaaagact aacaacgggg gatcaagtgc aaatagttgc tgctaaaaaa gtaagaggcg
     4021 gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt cctcctgagc
     4081 ctgctaaatt aagtaccaca ttgatcaatg cagcttctcg ctcaatcaca ggtacaggcg
     4141 aagcaggagc tttggtttca ttgaaaaaaa atcaagctgt tttgtccgaa acaactattg
     4201 atgatacagg taaatttgtt ctttctatcc
//
LOCUS       DAZKCC010000142         4161 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000142 DAZKCC010000000
VERSION     DAZKCC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.148"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..125
                     /locus_tag="ACG8VH_002884"
     CDS             15..125
                     /locus_tag="ACG8VH_002884"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111370.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
     gene            130..2259
                     /locus_tag="ACG8VH_002885"
     CDS             130..2259
                     /locus_tag="ACG8VH_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9111371.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            2256..3263
                     /locus_tag="ACG8VH_002886"
     CDS             2256..3263
                     /locus_tag="ACG8VH_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9111372.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            3280..>4161
                     /locus_tag="ACG8VH_002887"
     CDS             3280..>4161
                     /locus_tag="ACG8VH_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9111373.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKN"
BASE COUNT         1372 a          671 c          946 g         1172 t
ORIGIN      
        1 aaaacggtca atgcttgatg caagacttgt atgggcgagt aggtgtggtt caagttcatc
       61 cagtctttga agaactcttc catgcttttg acactcgtcc gccggttcaa gagagaatgg
      121 agtgagggga tgaaaaagaa actttttcgg attgtgggaa tcgtttttat tagcatcgtc
      181 ctattgacag ttctattgag tttagtcggt acggtagctg aggcgacagg tctggttgat
      241 gatacagttg aagccggaaa cctttattcc aaatattctc tgaacaatta tcaactggat
      301 ttctttgtgg atagttcttg ggattggtta ccttggaatt ggggggatgg cttagggaaa
      361 agcgtgatgt atggtcttta tgccattacc aattttattt ggacagtaag tctgtattta
      421 tcaaacgcca cgggctatgt ggttcaagaa gcttacaagt tggatttcat ttcggatacg
      481 gctaaaagta ttggaaagaa tatccaaact ttagccggta tcactgaaaa tggattacag
      541 acttcgggat tctactttgg gttcctatta ttgatgattt tagctctagg agtttatgtg
      601 gcgtataccg gattattaaa acgagaaact accaaagcag tccgaaccgt cattaatttt
      661 gtgatgattt ttctactctc gggttctttc attgcgtatg cgcccaccta tattacaaaa
      721 atcaatgact ttagctcaga cgttagtgaa gcggctctta gccttggaac caagattgtt
      781 gtacctaatt cagaaagcca gggaaaagat agtgtggatt tgattcgaga cagtttattt
      841 tcgattcaag ttcaacaacc ctggttacta ttacaattcg atgattccaa tatagaagaa
      901 attggcgagg gtcgggttaa taagatttta tccgtgagtc cggatgaaaa taagggtaag
      961 gatcgggaag aagcagtcaa agcagagatt gaagataatg acaatgcgaa tctcagtatt
     1021 accaagacca tgaaccgttt aggaaccgtg gtctttttag tgttgtttaa tattgggatt
     1081 tccttttttg tgtttcttct gacagggatt atgttgttct cacaaatcct tttcattatc
     1141 tttgcgatgt ttctgcccat tagtttctta ttgtcgatgt tgccgacgta tgagagtttg
     1201 ggcaaaaggg cgattattcg tttgttcaat acgattatga tgcgagcggg agttacctta
     1261 gtcattacga ctgcattcag tatctccacg atgtttttca atatttcggc cacctatccc
     1321 ttctttatgg tagcttttct acaaattgtg acctttgccg gtatctattt caaattaggt
     1381 gacattatga gcatgtttaa ccttcaaagt aatgatagtc agtcaatggg aaggcgagtc
     1441 atgcggaaac cgcaaatgtt gatgaatcga aaattgcgac agcttaatcg aaatgtgggt
     1501 cgaacttttg cttttggtgg cggtgcagca gttggtggga aactagctac cagttctaca
     1561 aaagggaaag gtagttcaaa aatcaataga gtgtccaata gaaagaacca ttccgcgaga
     1621 agcattaacg agctaaattc tgatctgaaa aaacaagaac aaccagtggg cacgaagaaa
     1681 gaatcacgaa taaatttagt tggtagaaaa gttggaaaag tactcgatac aaaagaaatc
     1741 gtgaaggaca aagcgaaaca agcaaaaaat caagtaaccg atgcaccaac taacttgaaa
     1801 tacagccttc ataaaggcat ggaaaagacg aaaaaagtac cagaggactt taagcgaggg
     1861 cctttcgaag aaaaagcaaa tcgaggggag ttgcgagaaa aacaacgaca acgaagagac
     1921 gcaaaaatgg atgaaaaacg caaagtattg aacgaagcat ggaatcgtca tgagaaagga
     1981 agggaaaatg ttccgataaa aaaggagata aaaccacaga aaaatgaact accagaaaat
     2041 aggtctttaa aaccaagggt acaagtacat tcgaatcctg aaatcaaacg gaaactttct
     2101 agtcaagaag tcattccaaa cggaagcact cagccactaa gcaagaacaa ttcctttcaa
     2161 caagtgaaac agagattcac ttttccaaag cgaaccaatc aacaagtaca aagaaaaaag
     2221 aacactcgcc caaaaccaaa gccaggtgag aaaagatgaa cttaaaaaaa ttgagtatta
     2281 tttcgttaac cttcctgacc atctcttttg tgggcatctt gatgtgtgtg ggtttgttgt
     2341 ttggtgagga cggtgaggga agtggtagtt ctggaacttc tcctggaggt agtagtgtct
     2401 cagaagtagt tttgaaacac cgttcaatgg tggaaaagta tgccaaagaa tcagatataa
     2461 cagagtatgt ttcgattctt ttggcgatta ttcaagtcga atctggaggt acaatggaag
     2521 atgtcatgca aagttctgaa tcagcaggtt tatcaccgaa ttcattaagt accgaggcat
     2581 ccatcaaaca agtgtgtctc tatttttcgt ctttggttaa gtgttccaaa gaactaggat
     2641 gcgatcaaga tagtatcatt caggcttata actatggtga cgggtatttg gattatgtcg
     2701 cgaaaaacgg aaagaagcat agttttgctt tagccgaatc tttttcaaaa gaaaaatcgg
     2761 gtggtcaaaa agtcgattat cccaatccca tcgcgattaa ggaaaatagt gggtggcggt
     2821 ataactatgg caatatgttt tattgcttat tagtgaagca gtacctaact acaacacagt
     2881 ttgatgataa aacagtccaa ggaatttttg acgaggcgtt caagtatgaa ggaacggctt
     2941 atgtatttgg tgggtcaagt cccggaactg ggtttgattg tagtggttta actcaatggt
     3001 gttatgccaa agttggtttg aaactccctc gggtggctca ggatcaatat gatgctatga
     3061 ctcatattga cttaaaagat gccaaaccag gcgatttgat tttctttcat tcaacttatg
     3121 atgccggtac ttatgtgact catgtgggca tttatgcagg agagaacaga atgtttcatg
     3181 caggagatcc ggtgggttgg accaatctta ccgatagcta ttggcaacag cacattattg
     3241 gtgctggtcg gtataaacaa tagaaaagag gaatggaaaa tgaagataaa aattgagcga
     3301 agtaacaagg aaaagattcc aaaaaagaag aaagaacgag tagtatccgt gggcaagcat
     3361 cgaaaaatgg tgcttgccct ttggctttta ttgtcttgta gccttgcttt tggaatctac
     3421 aaaaacttca cggccattga tcagcataca actcatgaga aagtggtcgt gaaagagaaa
     3481 atagtgaata cctctggagt agaaaatttt actgtagatt ttgtgaaaga atatttttca
     3541 tggaagaata acaaggaggt aattgaaaag agaatgacta atcttgagca gtaccttaca
     3601 gaagaagggc tcgctttgag ccaagatatg gttcgagcgg atattcccac tagttccgag
     3661 gttgtatcag taaaaattct agatgttgaa aaacactcgg aagaatttgt cgtttcattt
     3721 gtggtagatc aaaagattac agaagggaaa aagacacaat caatttcttc tgcatatcgt
     3781 ctgatgatct ttgaagatga aaaaggcaat catattgtaa cgagtttacc cactatgatt
     3841 ggtaagccag aaaaggcaaa acataaagta agacaagtaa aaagtgattc aaaaattgat
     3901 tcaaagacaa cggaagaaat caccgagttt ttagagacgt ttttcaggct ttatccagca
     3961 gcctcagaaa aagaactaga gcattatgtt gaaaaagacg cgatgcaacc aattaatact
     4021 aatttgaaat ttgtggaatt agtgaatcct atttacaaac aaaagggtaa agcgattcaa
     4081 gtgattgttg gtgttgagta tcttgatgag ttgtccaaaa caacaagtgt ttttcagtat
     4141 agattaaaac tatacaaaaa t
//
LOCUS       DAZKCC010000143         4045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000143 DAZKCC010000000
VERSION     DAZKCC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.149"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..1659)
                     /locus_tag="ACG8VH_002888"
     CDS             complement(154..1659)
                     /locus_tag="ACG8VH_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9111374.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1656..2342)
                     /locus_tag="ACG8VH_002889"
     CDS             complement(1656..2342)
                     /locus_tag="ACG8VH_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9111375.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2535..3956)
                     /gene="gndA"
                     /locus_tag="ACG8VH_002890"
     CDS             complement(2535..3956)
                     /gene="gndA"
                     /locus_tag="ACG8VH_002890"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9111376.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          856 c          691 g         1382 t
ORIGIN      
        1 tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt gttcgctagt
       61 ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct tattctttgt
      121 tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct ttgcgatagg
      181 aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga tacgtccttt
      241 atagttttca accagttgtt tagcgataga caaccccaga ccatttccac ccttcgttct
      301 agctcttgca ttatccactc gataaaaacg attgaagatc ttattaaggt cttctttggg
      361 tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg aagaaataga
      421 gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata tgataatgat
      481 taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca aatcatcatc
      541 taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt gataggtgac
      601 ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg ctcttgataa
      661 gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc tggcgcttaa
      721 tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac taagatgccc
      781 ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga attgctcttg
      841 ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag ctaaatctgc
      901 caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat ctttacgtat
      961 agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag ataaaaagaa
     1021 acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag tcaataatag
     1081 cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat agccgatctt
     1141 ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt ttccttctag
     1201 tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt
     1261 tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg gctgccctag
     1321 ctcagctata aaggaatcga tcttcattaa gtttccttcg accagtgaag tatgatctac
     1381 gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta ctaatgtcag
     1441 ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct ctacattgtt
     1501 tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag cgaaaattgt
     1561 aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca aggagggccc
     1621 tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac ataacctgtt
     1681 ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt gcgaagataa
     1741 cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca cactttattt
     1801 agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag cagcaactcg
     1861 tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg gttttctttt
     1921 tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt atcgccttca
     1981 atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat agcaaatggt
     2041 ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat cacagaatct
     2101 cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct gcagacttct
     2161 aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc attatttaat
     2221 gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc ttcatgcttc
     2281 aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag aatattgctc
     2341 atttattatt ttatcctttc tattctactg gctttttaaa aactctatca ttataccgat
     2401 aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa aagagctgaa
     2461 gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct catttcctaa
     2521 cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc ttctttgtcg
     2581 acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat atttgcaggc
     2641 agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt cggtacaggt
     2701 acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata ttttttcgtg
     2761 atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt gtcataggca
     2821 tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca gatttttgcg
     2881 atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat
     2941 ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt ttcgaccaat
     3001 tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt gcttgcttgt
     3061 acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt gatcaatgga
     3121 agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc tttattccca
     3181 gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc tttacgtgtc
     3241 agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt ccattcttta
     3301 aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa gtcataagat
     3361 tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt cacataatgt
     3421 ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc ttttgcagaa
     3481 atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc aggcatgatt
     3541 gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg
     3601 ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt atttcctccg
     3661 tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt tgcatctgtt
     3721 gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga ttctacaaat
     3781 tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac tacagcggtt
     3841 gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc tatgttcaag
     3901 gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt tgacatatga
     3961 actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa cacggctaca
     4021 ttgcaaacta aagaacaaca tattt
//
LOCUS       DAZKCC010000144         4030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000144 DAZKCC010000000
VERSION     DAZKCC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.150"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..775
                     /locus_tag="ACG8VH_002891"
     CDS             179..775
                     /locus_tag="ACG8VH_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9111377.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="ACG8VH_002892"
     CDS             899..2698
                     /locus_tag="ACG8VH_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP9111378.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="ACG8VH_002893"
     CDS             complement(2976..3188)
                     /locus_tag="ACG8VH_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111379.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="ACG8VH_002894"
     CDS             3650..3799
                     /locus_tag="ACG8VH_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111380.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1184 a          689 c          711 g         1446 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gagatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa
//
LOCUS       DAZKCC010000145         3961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000145 DAZKCC010000000
VERSION     DAZKCC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.151"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(609..1166)
                     /locus_tag="ACG8VH_002895"
     CDS             complement(609..1166)
                     /locus_tag="ACG8VH_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111381.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1345..1956)
                     /locus_tag="ACG8VH_002896"
     CDS             complement(1345..1956)
                     /locus_tag="ACG8VH_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111382.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2069..2326)
                     /locus_tag="ACG8VH_002897"
     CDS             complement(2069..2326)
                     /locus_tag="ACG8VH_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111383.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2923..3201)
                     /locus_tag="ACG8VH_002898"
     CDS             complement(2923..3201)
                     /locus_tag="ACG8VH_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111384.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3235..3486)
                     /locus_tag="ACG8VH_002899"
     CDS             complement(3235..3486)
                     /locus_tag="ACG8VH_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111385.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1201 a          810 c          526 g         1424 t
ORIGIN      
        1 tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa tggtgtccaa
       61 aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat tatctccacc
      121 aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat tttcatacaa
      181 aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac tagtatccca
      241 atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct tttatgtatg
      301 ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa caagttgttt
      361 ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca
      421 tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc tcagctaata
      481 gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat ttcaatggtt
      541 ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa tggattagta
      601 gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact aataagacgg
      661 attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc tatttcaggt
      721 gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt aggattaggc
      781 tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac ataatatatt
      841 cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc acttgctaaa
      901 tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat
      961 aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata ttctaatcta
     1021 aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa tattattcta
     1081 cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat gattaatatt
     1141 ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc tcacatatta
     1201 gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt cgctatgaat
     1261 tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat gatttggctt
     1321 gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg gttcagatat
     1381 ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt
     1441 aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc ttttaaaacc
     1501 ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta cattttcccc
     1561 catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc caaaaggtag
     1621 atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca tagaacctgc
     1681 gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt taatctgtcc
     1741 atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg gttcgggtat
     1801 tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca aaaaaactcc
     1861 tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc tcattgatcc
     1921 attgtattcc attttattcc ccctattact attcatatcc gcctatctat atcgatcttc
     1981 caatgcagag ataattgctt ccataccttg attttttact gagttaccta tgcgagatat
     2041 cattctccac taaggtgcat catattgtct atctaacatg attaattctc caataggtgt
     2101 atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct accatgtttg
     2161 ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct ggattaaata
     2221 taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct tttgaatgtc
     2281 cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt taccctgacc
     2341 attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc tcctaacttc
     2401 actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc atttaattcg
     2461 gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt atcaataaac
     2521 tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca taatatcatt
     2581 gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa
     2641 gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa ctatttgtaa
     2701 tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga ttcccaagca
     2761 aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc ttatataaat
     2821 tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg cttatatcac
     2881 aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt tttcaaattg
     2941 tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt taacattatc
     3001 atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta taattaacct
     3061 tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat ctctcctaat
     3121 ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat acatactgcc
     3181 tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc gttcttactt
     3241 ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca acaataagaa
     3301 tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat aagtaggatt
     3361 agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag cacaaacacc
     3421 aaagataaca actccaaaca ttgaattttt atacaatgac catttattta taggcttttc
     3481 tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag ttcataagcc
     3541 ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc ttgttttaag
     3601 cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg ccgaaaagtc
     3661 tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac tatttgtgaa
     3721 acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag tagctcttaa
     3781 ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat cccctctaaa
     3841 aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa tagagcatta
     3901 ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca attatttgta
     3961 t
//
LOCUS       DAZKCC010000146         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000146 DAZKCC010000000
VERSION     DAZKCC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.152"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG8VH_002900"
     CDS             complement(245..466)
                     /locus_tag="ACG8VH_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111386.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG8VH_002901"
     CDS             complement(564..809)
                     /locus_tag="ACG8VH_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111387.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG8VH_002902"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG8VH_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG8VH_002903"
     CDS             complement(2033..3034)
                     /locus_tag="ACG8VH_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFP9111388.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG8VH_002904"
     CDS             complement(3193..3459)
                     /locus_tag="ACG8VH_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111389.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG8VH_002905"
     CDS             complement(3449..3805)
                     /locus_tag="ACG8VH_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9111390.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKCC010000147         3725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000147 DAZKCC010000000
VERSION     DAZKCC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.153"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8VH_002906"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8VH_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9111391.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8VH_002907"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8VH_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1090 a          791 c          570 g         1274 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatccat caataacgtg ttttattttt taaaatttct
     3481 ataaaaaaac aatcattctt aaaaagaata tataaaaata acaataacct ttgtgatttt
     3541 tcttgtttga agaatgttag aagaaaaatc tctaaatact tataattatt ttataagcaa
     3601 aaaaatgcta tcggtagtat caccgatagc tgaggaaagt cacagtaggg actatttgat
     3661 taaaaaatat gtttattcaa tccatctatt tatttttgcc ttattaacat attgtaataa
     3721 tgtaa
//
LOCUS       DAZKCC010000148         3664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000148 DAZKCC010000000
VERSION     DAZKCC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.154"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG8VH_002908"
     CDS             <1..277
                     /locus_tag="ACG8VH_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFP9111392.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACG8VH_002909"
     CDS             420..641
                     /locus_tag="ACG8VH_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111393.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACG8VH_002910"
     CDS             657..1499
                     /locus_tag="ACG8VH_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111394.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACG8VH_002911"
     CDS             1562..3364
                     /locus_tag="ACG8VH_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFP9111395.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ACG8VH_002912"
     CDS             complement(3423..>3664)
                     /locus_tag="ACG8VH_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP9111396.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DAZKCC010000149         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000149 DAZKCC010000000
VERSION     DAZKCC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.155"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8VH_002913"
     CDS             <1..186
                     /locus_tag="ACG8VH_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111397.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG8VH_002914"
     CDS             329..553
                     /locus_tag="ACG8VH_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111398.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG8VH_002915"
     CDS             568..1437
                     /locus_tag="ACG8VH_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9111399.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG8VH_002916"
     CDS             1418..2152
                     /locus_tag="ACG8VH_002916"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9111400.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG8VH_002917"
     CDS             2185..3093
                     /locus_tag="ACG8VH_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9111401.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG8VH_002918"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG8VH_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKCC010000150         3563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000150 DAZKCC010000000
VERSION     DAZKCC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.156"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(180..857)
                     /gene="nth"
                     /locus_tag="ACG8VH_002919"
     CDS             complement(180..857)
                     /gene="nth"
                     /locus_tag="ACG8VH_002919"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9111402.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(864..1562)
                     /locus_tag="ACG8VH_002920"
     CDS             complement(864..1562)
                     /locus_tag="ACG8VH_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9111403.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1830..3014
                     /locus_tag="ACG8VH_002921"
     CDS             1830..3014
                     /locus_tag="ACG8VH_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP9111404.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3020..3463)
                     /locus_tag="ACG8VH_002922"
     CDS             complement(3020..3463)
                     /locus_tag="ACG8VH_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9111405.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          920 a          716 c          598 g         1329 t
ORIGIN      
        1 gggtattttc ccaataaaaa taacaacttt tatttttatc gcaataaatt gtaataagtt
       61 accatcctag ctactgagta tgtacattta ctttttcatg caaaaaagat aaacgaaaca
      121 gcttttctcc ataataacaa gataattcat acatttattt gcaattaaag aaaccacgtc
      181 tacaggcgtg gtttcttttt tagcgccttt tcttttagac gcatccgatt tttcccatct
      241 tgacatattg atagcaaagg acatacctcg cacttgggat ttcttgccgt gcaatgatat
      301 ctaccaaaaa aaatcagtgt atgatgtgtc tttacccata attcttgtgg tactttccgc
      361 atcagtgttt cttctacttc catgaccgta gcatctagtt tgcagatcct caggcgtttt
      421 gagacacgtt ccacgtgggt atctactgcg attgctggta tcccgaatgc atcccccaat
      481 acgacatttg ctgtctttct gcctacacca ggaagagaca tcaattcttc tcttgacgtt
      541 ggcacttgcc catcgaaacg ttcaatcagc tgctgtgcac acgctttgat gttttttgct
      601 ttatttcgat acaaaccaat cgtttttatt ttaagaatga tttcatcgat tgaagcttct
      661 gctaacgcat cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt attcactgat
      721 acatctgtcg cctgggcact taagatcact gcaatcaata actcaaatgg attattgtgc
      781 ttgagttctc catgtgcttc gggaaacatt ccatacatcg tttctagagc ttccattgtt
      841 ttttgtttgc ttaacatatt ccgctactcc tccaaccaat ttttcagtga gactttcggc
      901 aattcttctt gttgagctga ctgttctact tctctttgaa gaagctgctg ttttcttcgt
      961 ttttggtctt cttccacttg ttgttttgta cgtagatttt ttctttccca cgatagcaaa
     1021 atgcggtcaa cgtaattaaa actataagcc tgatttaata cggcttcccg cagtgctagc
     1081 ttcaaaattt ccggctgata atggtcttct tctagccatt gtccgatccg ctggaactcg
     1141 atcgaagaaa gcgggcgtcc aaattcttgt tcaaataatt gatacactga ctggatttct
     1201 ttttcttgtg ttttttcttc gtattttttt tcttgtgttt ttaaatactc acctaataga
     1261 tcatagatgg ggtaaagatt atatcgatct gcttttttac cgttgacaat cgtcgtttct
     1321 atcttgataa atccatttgt tacgagacga tccagtattt gaaaaatctg atcttgtttg
     1381 attcccatat ccatagcaat catctgcaaa tcagggaaag tatctccttc aagctgtgcc
     1441 atatgcaact gtagaacaaa taaaaattct tcagattgta gaccgatcct atgataattt
     1501 ttaagtaaaa gattagatat gactgtttgt ccagcgctta agtaattttc taaattcaac
     1561 atgatttctc ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag caagaacagg
     1621 tttcttttct atttacttca ctgatcggtc agatttacaa tgtcgtctac ttgatttttg
     1681 tttgttcatg tttttgatgc gtatcttcac ttttcggaat aaataaatta agtagatttc
     1741 gctgttcctt agaatgttga caaggaaaca aaattactat acactatttt tgtttccaag
     1801 ttaacattat tgcataaagg agtttttaca tgtacagttt cacaaataaa cattctaaag
     1861 tttttggata cgtctctgtc ttattcactg gattaggatt tactatcgtc agtcctgtac
     1921 ttccgttttt aacgctgcct tattctcact ccatacatca gcaagctttc tatatcactt
     1981 tgctcatgtc tgtttatgct ctagctgcct tcttatctgc ccccattctc ggtagcttaa
     2041 gtgatcattt tgggcgtcat ccgatcctta ttatcagttt attaggatca agtatcggat
     2101 atttgctttt tggtttagga aattcgatat ggatgttgtt ccttggtcgt atcattgaag
     2161 gattgacggc aggagaaatc tccaccttat atgcttactt tgccgacatc acggaaccta
     2221 atgaaagaac caaagttttt ggatggatgg gtgctttagt cggtatagga acaacgctgg
     2281 gtcctattat agggggtcta ttagctgagt tggggaattc tgttcctata tttattggag
     2341 cactctttac tttcttaaat gccgtttatg gctacacatt tatgctagaa agccttccaa
     2401 taaaaaaacg ttcagtagat ttaagtttct cccatgtaag accttttcac caattacaac
     2461 aattattcaa gatttcatcc gttattcctt tattgacagc aggttttgct gtttggttgg
     2521 cagctggctc tcttcaatct atcttttctc aattttcgat tgacgccttt cagtggaagg
     2581 ctgggttggt tggtttaagc ttctctttga ttggaatctt ggatactgtc tcgcaattgt
     2641 tcattatgcc tcgattgctc aaaaaatttt cagaacagca gatcacaaga atagggatgt
     2701 tcagtgaaat cctcgcctat ctattcatca cattatctgg aattcttcta ctgccgattt
     2761 tgtttttatt tgggattatc tgttatggtt tcggtgattc tatttttact cctgtattca
     2821 atggccaact atccaattct gtctcagata accagcaagg tcttgtaatg ggcggtaccc
     2881 aaagtataca atctctatcg cgcgtcatcg gcccgctaat cgcaggacag ctttatgctg
     2941 cagcaccatt tcttccgacg gtattcggat ttttgcttat gatttttgcc tattatcact
     3001 acaataaaaa atagtagctt catttgctac ttcgtataaa ttctctgtat cgaccgataa
     3061 aacgaaatac tgtgtcaaat tcttcttttg acatattttc aaatacagta gcatctcgct
     3121 gtacccattt ttgatggagt tgctcgtgtc tattccttat tttttgtccg attggcgtca
     3181 attgaaaata gatttctttt ttattctctt cgctttgata agtgtttatg atccctctag
     3241 ctatcagttt ttttgttagc ttactgatgg ctcctctagt catgtaacta gcattcgcta
     3301 attttgtcac gttaggatca gggattttcc cgatatgttc aatcacttct agttcattta
     3361 gagtaaatcc tgcaaattct ttttccattt ctggtttatt acgatgaata atctcattag
     3421 ctaaatccag atattcttct attagcagtt gtcttttatc catttctaca ccttcttttt
     3481 ctttcattat attgtttttt tgtttcctat tcaacaaaga taaaaatatt ttgtaggaaa
     3541 acccaatctg aaaccaagaa aaa
//
LOCUS       DAZKCC010000151         3489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000151 DAZKCC010000000
VERSION     DAZKCC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.157"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1838)
                     /locus_tag="ACG8VH_002923"
     CDS             complement(<1..1838)
                     /locus_tag="ACG8VH_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9111406.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
     gene            complement(1835..2575)
                     /locus_tag="ACG8VH_002924"
     CDS             complement(1835..2575)
                     /locus_tag="ACG8VH_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9111407.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(2565..>3489)
                     /locus_tag="ACG8VH_002925"
     CDS             complement(2565..>3489)
                     /locus_tag="ACG8VH_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9111408.1"
                     /translation="NIWTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAI
                     QKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTW
                     NNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDG
                     LINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDK
                     EQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDS
                     MVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT         1095 a          667 c          418 g         1309 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcac taggataccc accagtagat
       61 tctccacctc ccgatggacc accaccttgt ccaggaatta tttcttttcc tttaactgta
      121 agttttcctt gtacatctaa gtctccaaaa taaattgctt taccatttcc taataaaact
      181 aatccttttc ctactttagg agaaatcaaa atatatttgc cgtctccatt tgtacgaata
      241 actaaagaat tatcttcaat aggggttgga gtagaagccc caggaaaagg attaccaaca
      301 gaatcagttg ttccaatcgt tccaattgaa cccttagaat tccaaaattc cattcctttt
      361 ttagttaact ccattatttt ctttttgttg ttccaaattt ggagtagtcc attaactaat
      421 ttcaatacat ctccagtctt gttaaaagaa ttttgaaaga tatctgcttt gattaatcca
      481 gtttgtataa aattggcatt aaatatacta tctaatgtcc aagctgaatt aaaaggcccc
      541 cgccatcctt tcttagaaaa agcaatccca tttttattca ttcttagtac ttctcttgcc
      601 ttttctaaat ctggattatc cataataaag atgttggaag gtttttcttt tggccataaa
      661 actacatatc ctcctgcact gccttgacct gtaatcattg aagaaacata atcattaaaa
      721 tcactcatat aattattcgt tgcataatct tttaatttat cctgaatagt tacagcttgt
      781 tgctgataaa aagcaacttg aatgtctcct gcttctaatt tcaaagtttt ttcagataaa
      841 gaatcgtatt gtagtccact aacctttgat tcaagataaa tattatattt tttatgatat
      901 attttaaatg tatcaaacag accataattt ctaatcttcg caaattcttt cgcttcttca
      961 ctatcagtca acttatcaaa ttcaaccgta atagaaactt taggcttatc acagccagga
     1021 ttgatcgttt taaagtaatt tttagctatt ttatttaagc tcttaatgtc ttttactcct
     1081 tgttcttctg taaactgaac atgttctgta tagacatcag gatagttatt aatataggca
     1141 ctatctactg gcgaaccata aattcgatta gtcgtaccta cttcactttg aggatcagca
     1201 tatggaataa ttcttgtttt tatacctgtc caatctaatt tgaccttcaa accagacata
     1261 tcttttccat accgaatcgt tccaacatta ttgcgacctc ttcgctttaa caaagaaagc
     1321 ttaaatggtt cacgtttaat ttctccgccc caatattgaa gcaatgatcc ttgttcacca
     1381 gcaatacaat ttaaaacatt tctcgcttca aagattgtgc tagaaacagc cgtaatatca
     1441 gaataaagtc gtatgtcaga ttttttatcc attttagttt caataattga tatcgcttct
     1501 tgaccagttt tagagtccac ttccacaaga gtgactaccc gtcttccaag tcgatttgta
     1561 cgactctgag cataaatagt tactgtattt aaaaaagtat ctatatcttt atcatcaata
     1621 aaaaagatat gatactcttc ttgatcgttt ggctttgctt ttatttgata gtcattttca
     1681 aaatattcat caaatctagt tcctaaagga taatccaact ctaattcata ttttccatta
     1741 gctacttcat atattccaca tcttgtacaa tcctttaaaa ttcctaagcc attcgttgag
     1801 aaatctgttt cagtaggact atatattctt ggtttcatac ttttctccac cacctaggca
     1861 ttatttcaaa tgaatgaata tttttcgtcc atttaatttc attttttcca ggatataaaa
     1921 atggaaaatc taaaaataag gtgacctgat cctgatgttc taaattacca tctaattttc
     1981 gataagcctc ttctaattta gaatctataa ataattctct gtccaacgat ttcaaatcat
     2041 atttatcctt attaatataa aaagaagcat ctccagaacc actcaattta ataagcggtt
     2101 ttgaagaata cttctctggg ttataaattt caaaagtttc tgtttgtcga atagtaaatc
     2161 taccattgta attttcctta aatggtcgaa tactcactgt aaattcaaaa gggacaatat
     2221 ttcctgtttt tcttgttcct ttaaattcag gtccttctat tactacagct tgataaatat
     2281 attgttcatc atagtaaaga ataaaatcac tataagacga catatctaac cattcagtga
     2341 ttcgatcttc ccactcctgc accatatcaa ttgaaggtgc tttatagtaa cactcaatct
     2401 ttctagtcac atttttgtaa tagcctttat caataatgat agaatcattt ccttctcgct
     2461 ctctcaattc aattactcga ctagctgaaa cagaagcagg cctgttttga atatacacat
     2521 taaattcaga agaataatgt tgattaataa aaaattgtcc tctcttaagt tgcatataat
     2581 gttcctccta ctgcaccagc atctcgtttc atttgtcttg tcaattctgt tttaattttt
     2641 gtggtaatct tccgaaccat tgaatcaggt aaatctccat aaacatttaa atgtaaatga
     2701 atttcttttg tttcactagc agatatattt tgctttgttt gtgtaattgg taggtgactt
     2761 attccattta ctacaggttg aacagacggt gtttttacta aatttgtcat ggtttgatct
     2821 aacttacttt gctctttatc aataccaacg ataatacctt gaacaatatt tttaccaacc
     2881 atatcacgca tccatcttga tggagaatga atatctaaag ctcctttaat ccaatttttg
     2941 atattacctg caataccttt gatagtatct ttcaaagcat tccacttttc ttttacacca
     3001 tttataagtc catcaatgat gttcttgcca atttcaaata aattgacttc ccttaacgaa
     3061 ttaaagattt cttttacccg attaatagca tttgaaacac cattttttag atttgtccat
     3121 gcattttcag cagaattaac aattccagta acaatattcc agaaagagtc cttaatgtta
     3181 ttccatgtat taatcatggt attttttata gaaatccacg tattgtatgc cgtatcttta
     3241 atatttttcc aagtatcttt gaaaaactgt ttaatattat tccaagtagt aatggcatta
     3301 tattttaaat cgatccaagt ttgaataata gaaaacttca attcaatcca tttctgaatt
     3361 gcaaaatatt ttatatcaat ccaaagatta ataaaaaaat actttatgtt taaccaaatc
     3421 gatttagctt gatttaccac ttcattccaa atatttatca atgtaagctt gaaccctgtc
     3481 caaatattg
//
LOCUS       DAZKCC010000152         3372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000152 DAZKCC010000000
VERSION     DAZKCC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.158"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1188
                     /locus_tag="ACG8VH_002926"
     CDS             109..1188
                     /locus_tag="ACG8VH_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9111409.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1489..1731)
                     /locus_tag="ACG8VH_002927"
     CDS             complement(1489..1731)
                     /locus_tag="ACG8VH_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9111410.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(1763..2320)
                     /locus_tag="ACG8VH_002928"
     CDS             complement(1763..2320)
                     /locus_tag="ACG8VH_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9111411.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(2333..2521)
                     /locus_tag="ACG8VH_002929"
     CDS             complement(2333..2521)
                     /locus_tag="ACG8VH_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9111412.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(2534..3100)
                     /gene="amaP"
                     /locus_tag="ACG8VH_002930"
     CDS             complement(2534..3100)
                     /gene="amaP"
                     /locus_tag="ACG8VH_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9111413.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT          930 a          768 c          684 g          990 t
ORIGIN      
        1 gaggcggatt ttataattaa cttagccatt tggaaataaa aaaacgccac gtgaattcca
       61 tgttatattg aagttaccac aactctcaat agacaaggat gaattcacat gacgcaaaac
      121 catgataaca cagtttcacg caagggaaaa cacctaacct actcagaacg ttgccaaatc
      181 gccatcctga aaaaggaaaa ctattccaac cgtcaggttg cggatgtcct gggctgcgtg
      241 ccgcagacca taaacaacga aatcaaacgc gggaccgtta ctcagcttaa ccgccagaat
      301 cagaacggga aggtctacga ctattatcat gacgtctacg acgccgatgc cgggcaggcg
      361 gcctacgaga agaaccgctt gaactgcggc agacggccct tatgggccga cacggacgcc
      421 ttcattgaat gggcggacga catgatgctg ttggaaaaat ggtccccgga cgtggtggtg
      481 ggctttgcca gaaaacacga attgttcgac ccggccatta tccctgggac caccaccctc
      541 tatcagtgga tcgaccgtgg catcatgaaa acgaagaata tggacctgct agaaaaactg
      601 tcccgcaaac cgaaatcgtc ccgtcatggc aaccgcccga ataaacgggt gctagggaaa
      661 tccattgaag agcgcccgga ggcggttggt acccggaaaa ccttcggcca ttgggaagtc
      721 gacacggtcg taggaaacaa gaccaagtcg gatgccgtgc tgctgacgtt ggtggaacgg
      781 cagacacgct tcgaggtcat tctgaagctg gccgggaagg atgccgaatc ggtcaaccaa
      841 gcggtccgac agctgagaga acgggctggt gcgtacttcg gccagctgtt caagacggtt
      901 acctcggaca acggctcgga gttcgcggga ctgcacgatg ccttgcagga tgtggcaaac
      961 atctacttca gccaccccta tgcttcctgg gagcgcggga cgagcgaaaa ccaacacaag
     1021 ctgattcgcc gtttcatccc caagggcagc ccaatcggca acgtgagcga aaaccaaatc
     1081 ttgcggattc agcgttggat gaacgattac ccgcgaaaga ttttgggcta tgcaacgccg
     1141 catgacacgt tcgtccaggc atttaggcag gaacggttga cggcttgaca cgtgggccct
     1201 tgacaacgag cctccctcgg catggcttgt gtctggaaag ctggccgggg ccagccagtc
     1261 gggagactac catgcctcag ctcattgtca agggcccact cgattagaaa aattagacgg
     1321 ttggtggtaa cgagcaaccg ggtcaccatg ggtggctaac ttaaacttga aatttacgta
     1381 aggaataaag aatacttcat ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg
     1441 gttctcaaat ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg
     1501 tgcaaagaat gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc
     1561 cattcctgca attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac
     1621 taaaccagca atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc
     1681 tgcaattgct ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc
     1741 ctcctaactg acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt
     1801 gtgcaccttt gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg
     1861 tggattttgc tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt
     1921 cttgtgtttt gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat
     1981 tgtttacttc agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac
     2041 cgtattctac gatcacatct agatccacag ctacttgttt cttacctact tctgtattga
     2101 tacctgctgt tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc
     2161 catctaatgt caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga
     2221 ctttgtcttc ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca
     2281 aaactgatcc atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt
     2341 taaaaaattg atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg
     2401 ctgtcatgat tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta
     2461 gcagtagtcc aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca
     2521 ttatttggcc ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt
     2581 cataaccgct gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta
     2641 cttcttcttc gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt
     2701 tcaagtctcc gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag
     2761 gtgaatgaat gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt
     2821 tgtcgattgt tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat
     2881 agaaaagaac gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga
     2941 aaatatattg ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt
     3001 caggcataat atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta
     3061 aactcatgat aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt
     3121 atttttaccc tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat
     3181 agcatcagta aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag
     3241 tctgatactg aaataacaat tgcttactta ttctctattt acttttgatt gctggctata
     3301 cttaaaggat attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag
     3361 attctattta tt
//
LOCUS       DAZKCC010000153         3352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000153 DAZKCC010000000
VERSION     DAZKCC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.159"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG8VH_002931"
     CDS             <1..546
                     /locus_tag="ACG8VH_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9111414.1"
                     /translation="MNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLIL
                     FLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSF
                     ARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFF
                     LYPLSKDKVLENCELLKQKHK"
     gene            556..1350
                     /locus_tag="ACG8VH_002932"
     CDS             556..1350
                     /locus_tag="ACG8VH_002932"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9111415.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1363..3129
                     /locus_tag="ACG8VH_002933"
     CDS             1363..3129
                     /locus_tag="ACG8VH_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFP9111416.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            3152..>3352
                     /locus_tag="ACG8VH_002934"
     CDS             3152..>3352
                     /locus_tag="ACG8VH_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9111417.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGW"
BASE COUNT         1206 a          397 c          660 g         1089 t
ORIGIN      
        1 atgaatttta tcaatcctat tttggtttta atagttgttt cgcctttaac tttgttttta
       61 agtaaaaaat atggaaaaaa agaactggca tcagtaggaa tgttcttttc agcaatagta
      121 tatttgatct tatttttgat aaaagctagt aatatgtatg tattcttaat tcttagttcg
      181 gttggctata tgggtttggg tgtgtttaat actgttattt gggcaaatat tactgatgtt
      241 attgatgatc aagaagtaaa gtctgatcaa agagaagatg ggaccgtata tgcggtatat
      301 tcttttgcta gaaaaatagg tcaagctcta gctggaggcg tcggaggttg gactctttct
      361 attataggct atgactcttt agcaaatgta caaacgggag atgttttaga aaagttatat
      421 aacgcttcaa cattaattcc atcaatttgt tttctgattg taggattaat tttatttttc
      481 ctttatccat tatctaaaga taaagtctta gaaaattgcg agcttttaaa acaaaaacat
      541 aaataggaga gaaatatgaa taattggata ttttgtattg attcagatgg ctgtgttatg
      601 gatacgatga cttataaaca caatctcttt ttcggaccaa ttgctgcaga gatttttgat
      661 gttcatcata gagaaaaatt cctacaggat tggaataata ttaatttata ttcagagact
      721 cgtggaataa acagattttt aggtttagtt aaaacattag aaaaaaatga tgtaacatcg
      781 attaatcgat taaagcactg ggtatctcta acaaaagaat tgtctaatcc atctttgaag
      841 agggaaatag aatattctca gtcagaggat ttattaaaag ctttaaaatg gtcggaagaa
      901 gtcaatcaac agattagtca agcaaaagga ttagataaac cctttttaag tgctagagat
      961 actataaaag ctctgaaaaa atatgggaag ataattattg taagctcagc taataaggaa
     1021 gctgttcaag aagaatggga aagacatgaa ttattatcat tagttgatga actgtgttgt
     1081 caagataaag gaaaaaaaga agatattatt cgaagtgtaa ttgaaaatgg ttgtgatttg
     1141 gataagatat taatgattgg tgattctcct ggtgatctag aagctgcaaa taaaaataaa
     1201 gttttctttt atcctatttt ggtcaataaa gaaaaagaat catgggaaaa cttaagaact
     1261 gatgttttat ttaaatgttt atcgggagat tatgtagata ttgaacaaaa atatatagat
     1321 actttttgga aaaatcttac taataagtag aggagaaaga aaatgactat aaaagtagat
     1381 ttacgaaaaa aaccatataa tttagatgaa gatgctattt cttggattga agagacgatt
     1441 gattctatga ctttggaaga aaaaataggt caattgtttg ttaatatggg atctagtcgt
     1501 gataaaaatt acttgagtaa tatggtcaat cagtaccata ttggagctgt acgatataat
     1561 ccaggaactg ctgatgaggt atatgatcaa aactatatct tacaaacaga aagtaaaatt
     1621 cctctattaa ttgctgctaa tacggaagca ggtggtaacg gagcttgtac agatggtact
     1681 gaagttggtg tagaagtaaa aattggtgca acaaatgatg cgaaatatgc ttatgaatta
     1741 gggagagttt ctgggattga agcatcggct attggatgta actggagttt tgctcctatt
     1801 gtagatatta atagaaattg gagaaatcct atcatttcta gtcgatcttt tggacaaaac
     1861 cctgataaag ttttagaact tagtttagct tatatgaaag gtattcagga gagtggaatt
     1921 ttgcctgcag ctaaacattt ccctggtgac ggtattgacg aacgagatca gcatctatct
     1981 tttagtatta atagtttagg tacggaagaa tgggataatt cttttggaaa agtatactca
     2041 ggcttaatag aagctggatt accttcaatt atggcaggtc atatccatat gccagcttat
     2101 tcaaaacatt ttaatcctga tctgaaagat gaagatttac ttcctgcaac tttatcaaaa
     2161 gaattaatca ctgacttact tagaggaaaa ctcggattta atggattagt agttactgat
     2221 gctagtcata tgttaggaat gactggggca atgaaacgat cagaattatt gcccacttct
     2281 attgcagctg gatgtgatct atttctattt tttaatgatc cagatgaaga ctttaattat
     2341 atgatggaag gctatcgtaa aggaattatc actgatgatc ggttattaga tgccttaaaa
     2401 agaattttag ggttaaaagc taaattggga ttacacaaaa aagcaaaaga acaaatttta
     2461 ttacctaagc aagaagctct ggaaaaaatt ggtttaattg aaaataaatc aatttttaaa
     2521 gaggtggctg accaagcaat tacattagtt aaagataaac aagatatttg gcctattgta
     2581 ccagaaaaat ataaaaggat tttactagta gatgtacatg gggtaaaagg tgggtttggt
     2641 gaagtagttg ctggaaatag acctcaagcc attgatttat tgaaacagat tttgtcagca
     2701 aaaggatttg aagttgaaaa atacatttca cctgaagaag aattgaaagg attatcgcaa
     2761 gaagaaaaaa ttaaaaatat agctggaata tatgctgcta agagaccaat taaaaattta
     2821 actaaaaaat atgacttgat cattaatata gctaatgtta atccaggcac agttcaacga
     2881 attgtctggc cagcaagtaa aggaactccg gatataccgt tttacattca tgaaattcca
     2941 acaatttttg tatctgtaca gtgtccgttt catttggctg atgtacctca agtaaagact
     3001 tatattaata cttatgatgg aaaacaagaa actttagaaa tattggtgga caaactaatg
     3061 ggtctttctg aatttaaggg agtaagtcca atagatgctt attgtggttt aaaggataca
     3121 tttatttaat atttagatag gagtggttta tatgttgtat cccattatga cagagacgag
     3181 gcaagtaatt gacttaagcg gtctctggag atttaaatta gatacaaatg atgacttaca
     3241 ggaagaactt gctttagaac caataaatga aaaagagagt tatccgattt cagttccctc
     3301 ttcttataat gatttatttg aaacagagaa cattagaaat catgttggat gg
//
LOCUS       DAZKCC010000154         3304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000154 DAZKCC010000000
VERSION     DAZKCC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.160"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..243)
                     /locus_tag="ACG8VH_002935"
     CDS             complement(<1..243)
                     /locus_tag="ACG8VH_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9111418.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
     gene            complement(260..1732)
                     /locus_tag="ACG8VH_002936"
     CDS             complement(260..1732)
                     /locus_tag="ACG8VH_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFP9111419.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            complement(1746..2594)
                     /locus_tag="ACG8VH_002937"
     CDS             complement(1746..2594)
                     /locus_tag="ACG8VH_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9111420.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            complement(2591..>3304)
                     /locus_tag="ACG8VH_002938"
     CDS             complement(2591..>3304)
                     /locus_tag="ACG8VH_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9111421.1"
                     /translation="NFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNR
                     KGNIFFKVAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAM
                     YTLVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAI
                     FILLTTLISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTT
                     MIGLITIAQRKLVKEDDPA"
BASE COUNT         1024 a          707 c          604 g          969 t
ORIGIN      
        1 tttcggggtc gaggtaatgt acccataagt ggcggaatcg cgatggatat cgataggccc
       61 aaatgagcca atcccaatcc ctgctaaatc tgcttggtat tgtttgaaga agtcaatgac
      121 taaaggtatt gtttcctcag gtgtcgttgt cggaaagctc actctttcct tgatcgtcat
      181 ttcttcatcg ctaatggcac acacaaattt tgtgccgcct gcttcgattg aaccataaat
      241 catcactgag ctctcccttc tatagattaa ttaagccgct ttccaatgaa aaggttactt
      301 cactgtctgc cagcaaaaga aaatcctgtc ctttttctgt ctcataaaaa gttgtactca
      361 tagtcagacg atcatttaga aaaagctcaa tagctgaagt atcacggaaa atttcgatgg
      421 ttagacaatt attttccaaa ggaatcgtca cactgcgctt tttcaacact tccttttcag
      481 ctcctgataa atcatacggc atgtgttccc tggaagcagt aaagcactcc cgttcaacat
      541 catagttaag cagtaatgta tggtcgggtc ccttcgcata gtgaagttct agtttcgttg
      601 cctgttttaa attgatttcc aagcgtaaat agtgctggtc atatactaag tccttcagta
      661 agaagcgatt ctcttcagtc ttcgcccatt caatcctttc ctgcggatca attacttgat
      721 aaattgatga aactggccgt tgcaacaatt gattatcagc caccctcagc tctctaggca
      781 aggtcatgga acctgaccaa ccatggccca gatcattcgt aggtatttta cgctgccaca
      841 tttgcatcca agcaatcatt attctttggc ctgcttcgtt ttcacaggtc tggggtgcat
      901 aaaaatctaa accgccatcg atttcatgaa aattctctac tagaaatttg cctgtttgcc
      961 aatcgacagt accgatgaaa gcaacagttg agttcgtatt ttggtaagca ttgccttgtg
     1021 gagtcatttg aataggagaa agaatcaaga cttctttgcc atctagttca aacaagtctg
     1081 ggcattccca tacaatgcct tgttctggag ttccctccag caatacggaa taaaactccc
     1141 aagttagtaa atctttggaa tggaacatca caatttgtcc acgagcatct gcagttttag
     1201 tagcgactac agtaaaatag tcttcttttc gctgaaacac tttgggatcg cggaaatctt
     1261 cggtcgttgc aacagaagct aagtgatctt ctgcgatgat tggatttccg gcatattttt
     1321 caaagtgaat gccatcttct gaaactgcca aacactgaac ttgtcgaact tgcccttcca
     1381 caacattgtg tcctgtataa atcaaataca gcttgccatc cttttctatg gcactgccgg
     1441 aaaaacagcc ttccttgtca tagctttcac ctggtgccag tgctactggc aaatgctccc
     1501 agtgaattag atccttgctt ttagcatggc cccaatgcat tggtccccag atgctgtcgt
     1561 aaggataata ttgataaaac aagtgatact cctcttgata ataaacaaaa ccatttggat
     1621 cattcatcca gccaatcgga gccatcaaat gaaatttatt ccggtattct tgattgactg
     1681 tttgagcatt tttctcaata aactcattgg cctttaaaag tttttgcaac attcactatt
     1741 cactcctatt ttatacccgt tccagaaccg ccaagtccct gcagaatata tttttgtgca
     1801 acgatataaa caataatcag tggaatcgtt gcaatggtta aaacagccat cacttgattg
     1861 atataaactg gctgtgtagt attgatcgta gtaattgcca cttgcattga aaatttgctg
     1921 ctgtcagtta agaccattaa cggccaaata taatcattcc agctggagat aaaggataag
     1981 acacctactg tggctatggc tggtttagac attggcagca tgatgtgaaa gaaaatttta
     2041 acaatatttg caccatctaa tttcgctgat tcaatgattt cttctggaat cgcgataaag
     2101 aaatttctaa acagatagat attaaacgcg ctggccatcg ctggtaaaat cactgccaag
     2161 cgagtattca ctaagcctag tgaattaacg atggtaaatt gcggaattaa aaccgtctcc
     2221 atcgggacaa tcagcagagc taacaaagct ccaaagataa ttttcttgcc tgaaaaatta
     2281 accttcgcaa aggcaaaacc cgccaaagag tttacaatga ttgacccaca ggcaaaggtt
     2341 aaaccataaa aaatactgtt taataaataa gttcccacac tgaaccggga aataacctct
     2401 tgataagttt taaaccaatt agctggattc aaactaggta aaaaagcttt gaaggaagct
     2461 aaattttgat agacatctgc ttcaggcttc atagaggaaa ctaccatcca cagcatcggg
     2521 aaaatgaata atgccgctaa aaggaacagc aaacaatatt ctacgatcat tagcggtcca
     2581 aatttcttgg tcatgctgga tcatcctcct ttaccaattt tctttgagca attgtaatta
     2641 aaccaatcat agtggtaaag attaacgcaa tggagctgga gtagcctacc atacggtctg
     2701 taaatccagt ttggtaaatg taataaacca ttgtcatagt agaattcatt ggtccacctt
     2761 gagtcattac cattggctga atgattagtt taaaggccga aatcaatgtg gtaagtaaaa
     2821 taaaaatcgc cgtgggcttt aataatggca tggtgatata gcgaaattgc tgaaacttag
     2881 aaaaaccatc aatttcggct gcctcataaa cgtcttgcgg aatattttgc attcccccta
     2941 aaaacagcag catctgatat cctgcacctt gccatgccga tacgaaaaca agcgtataca
     3001 ttgcttgttt gggactagtc aaaaaaggct gcgaagcaat tccaatcttt gcaaatagcg
     3061 tattaaccag accttcatta ggatttaaca agtacaacca taaaatcgaa ataacgacta
     3121 aggacatcac gactggcgca aaaaaggcta ctttaaaaaa gatatttcct ttacgatttt
     3181 tgttgataat aagtgccatt cctaaagctg cccctatttg gaccggaata atccaaacaa
     3241 caaatttagc cgtgttccaa atacttttta caaaaattgg atcagcgaac agtcgcttaa
     3301 aatt
//
LOCUS       DAZKCC010000155         3283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000155 DAZKCC010000000
VERSION     DAZKCC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.161"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..347
                     /locus_tag="ACG8VH_002939"
     CDS             111..347
                     /locus_tag="ACG8VH_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP9111422.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            366..581
                     /locus_tag="ACG8VH_002940"
     CDS             366..581
                     /locus_tag="ACG8VH_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFP9111423.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1284..2036)
                     /locus_tag="ACG8VH_002941"
     CDS             complement(1284..2036)
                     /locus_tag="ACG8VH_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9111424.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2051..>3283)
                     /locus_tag="ACG8VH_002942"
     CDS             complement(2051..>3283)
                     /locus_tag="ACG8VH_002942"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9111425.1"
                     /translation="FFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVSAAIPI
                     IGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGYVVFQI
                     MLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHF
                     VFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFAR
                     FRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKS
                     LLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELS
                     LAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMF
                     VNYQNDPYQFTTQVILLF"
BASE COUNT         1131 a          506 c          499 g         1147 t
ORIGIN      
        1 ttggaaattt tactattcaa gaataaactt gataagtttc aggaagattt ttactgttaa
       61 tcaaatacgt atgttacagt agcaccgtaa aaaatattat ttaaagaagc gtgtttaaac
      121 atgcaaaatg taaaggaact aagtattgtg gaaatgcaca aactgttggt ggaggcaata
      181 atgcttgggg aaaattgggg caagttgtag gtggacttac cacaggggca gtaggtggtg
      241 caggtttagg aacagcaatt tgcggaccag cttgcggtgt tgttggaggg ttatatggag
      301 ctgtcgctgg aggtgctgca gctggctggg atgctcgaaa aaaataaaaa aggagaataa
      361 gtagtatgca tattaaaaac acaaaaacaa catttatatt atcttcagaa gagttgaaaa
      421 atattcaagg tgggagtgcc gtgggagttt taggcactac tttttctggg gcaacagcag
      481 gagtaaaact ttgttcagcc ggaggacctt atgcaatagc tgcttgtggt gtaggtggag
      541 ctttattagg tgctggattc agcatgtgga ccggtgcgta aacaattaaa aaatatcaaa
      601 cctcaagatt ggtttgtgtc gattacgaac ataggtatgt tctttatctt ttcaaaagtt
      661 tggaatggac agaacttttt actaaagctt cttgtgctag tttttataat tatattaaat
      721 ttttatcttt tattaaagtc agataaaaat tctttggaaa tgtaatggta gagatatttt
      781 tacaaaagga cttgcagcgc tgtagttggt gctgcaggag gacctatttc ggccgttaca
      841 ggaggtattg gtaatgtact tgtcggatgt gcattaaagc ctgttcctgc aagttaaatt
      901 tataaaaaca ggaaagtaat tatctcaaat cctatcaagt acaatcatag gattgcactt
      961 gacaggacaa attttttaac taaaaaatga aaaacacttt ctaaaaatta gtagaatttg
     1021 ccaaatcttt cgcatcttcc accatacatt taattttaat tcccctttat ggtacttaat
     1081 aaaaacaaga aaagaactat tcatttggcc cttttttggt tccagaccat agaaatattc
     1141 ctccagtaac taatattgct gttatgcacg taactaataa cagctataaa aattggtaca
     1201 actttaatag gattcagaaa aatttgcact agtgaaggaa tctattttat tagaaaaatg
     1261 caatctccct aaagaaaaaa ccactaaaca gtgctttctt tcaacttctc cttaattttt
     1321 tgtgctttcc ctaaagtaaa gtttctggcc aactctgaat caaaatatag tgaacgtttt
     1381 ttaaaatcta tttctttaac gttaaagata tttactaaac acgctcgact aattcttgtt
     1441 aacataggat atttcttttc aagctcacta attcttccat aaaattcgta ctgcccatct
     1501 ttagtacaca atgataatct atgtggtaat tttgaagctt ctacaaaata aatgtcattt
     1561 atatcaaaag taaatgtttg agaacctatg gaaaaaacaa atgcttgatt cgtaacctgt
     1621 tttgttgcat ctatcctttc ttgcgctaat aacaataact caacaatctc gtttcgatac
     1681 tcatctagtg tttgatcttt tgttacaaat cctaacgttt caactcttct ttttattgtt
     1741 acgggtaaca tctcatcatg agtagttgta aaaatgattt ttgcttgaac atcatatttt
     1801 ctgattactt ctgctaattc tataccgtta acttcatgat ttaaatcaat atctaagaaa
     1861 tatattcctt gctttggtcg gaattgtttg aggtattttt taacttctaa cggactttgt
     1921 gttttcaata ctatcttaaa tactttatcg tgaaaaagaa tgaaattatc aataattcgt
     1981 tctaactgat ttaattgtat aatttgatct tcacataata ttattggata actcataaat
     2041 cctccttccc tcaaaacaac aaaataactt gagtagtaaa ttgatacgga tcgttttgat
     2101 agttcacaaa catattggga ttctttttat tgatttcctc tatactagat aaacctaacc
     2161 cttgatgtcc cttttttgtt gttattcctt ttttcattag agtatctata gagagtagta
     2221 tctctctaca actattcgat atcgaaattt ctatctgttt atcatctcgg taaatagcta
     2281 gagacagttc tctatcttga ctctctatag atgcttcaat tgcattatcg agaataattc
     2341 caagcaccct aatacagtca aatattggaa caggaacttc aataatagta cttggacatt
     2401 cgaatttaca acaaatctta ttgttatttg cttggtatag cttcgctatc aacaaacttt
     2461 tcaaatattc attttttata ttttttaaat aacgatattc aaacttattg gttagataag
     2521 aattagaata gatctctaat tcttttattt ctttttccaa tatcgaatca tggttttgat
     2581 tagcgatttc ttttaaactc agcaataaat ttttatagtc atgtctaaat ctggcaaatt
     2641 tttcttgatc tttttccagt gaatcagtat atttttttag ataaaccaat tcttttcttt
     2701 tcaattgctg ttcatattgt tctcgttgcc taatagttat tctaataaat aaaaatagaa
     2761 caacaaacat ttgaatgatt aaaaatatta agacaccaaa cacaaaatga tcaaatactt
     2821 gatattgatg agcaacataa gaaacaaata aaccaattgt aagtaaataa atcatgaata
     2881 tagcagtaag tttagaactg gactgttcaa caatcctatt tatagctaat tttctatata
     2941 accaaataaa catcagcaaa aacaaagaat caagcattat ctggaatact acataaccat
     3001 acccttttat cccttcaaat gaaaacatat acgtcataac catggaagta ataatagatc
     3061 tgaaatcatc aataagcata cataatagta atttatttaa aagcactata ttagttttct
     3121 tcttttcctt taatataaaa taacttccga ttataggtat agctgcggaa acatcagtaa
     3181 ataattcaac aaaaaaagtg agtggtatca ataaacatac aaaaagatat tttcttttgt
     3241 taagatcagt attagtcata taaaataaga gaaaaaagaa aaa
//
LOCUS       DAZKCC010000156         3201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000156 DAZKCC010000000
VERSION     DAZKCC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.163"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8VH_002943"
     CDS             301..585
                     /locus_tag="ACG8VH_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111426.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8VH_002944"
     CDS             588..1004
                     /locus_tag="ACG8VH_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111427.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8VH_002945"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8VH_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9111428.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2523..2609
                     /locus_tag="ACG8VH_002946"
     CDS             2523..2609
                     /locus_tag="ACG8VH_002946"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9111429.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2912..>3201)
                     /locus_tag="ACG8VH_002947"
     CDS             complement(2912..>3201)
                     /locus_tag="ACG8VH_002947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111430.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
     2881 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     2941 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     3001 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     3061 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     3121 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     3181 ttctttgccg tctatactct c
//
LOCUS       DAZKCC010000157         3105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000157 DAZKCC010000000
VERSION     DAZKCC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.164"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..785
                     /gene="fabI"
                     /locus_tag="ACG8VH_002948"
     CDS             30..785
                     /gene="fabI"
                     /locus_tag="ACG8VH_002948"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9111431.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(895..1947)
                     /locus_tag="ACG8VH_002949"
     CDS             complement(895..1947)
                     /locus_tag="ACG8VH_002949"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP9111432.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2073..2993
                     /locus_tag="ACG8VH_002950"
     CDS             2073..2993
                     /locus_tag="ACG8VH_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP9111433.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          906 a          632 c          597 g          970 t
ORIGIN      
        1 atttttatca gaaacaaagg agagtcatta tgtcatttct agaaggaaaa aaaatcgtca
       61 ttatgggcgt cgctaacaaa aaaagcattg cttggggttg tgctgaagca atgttgaaac
      121 aaggggcaga aattatctat acttaccaaa atgaacgaat gaaaaaatca ttagtcaaat
      181 tggtcggcga agaagcattc actgtagaat gtgatgtttc tagcgacgaa agcatcgaaa
      241 aagcaatgaa tgccatcggt gaatatgctg gggagatcca cggccttgtc catgcagttg
      301 cttacgccaa caaagaagaa ttgtccggaa atgtgtctga tatttccaga gacggctatc
      361 aattagctca agatatcagc agctactctt taattgctgt atcaaaatac gcacaaccgt
      421 ttcttgcacc aaacagcggg atcgttagca tgacttatct aggttcagaa cgcgcaatcc
      481 cgaattataa catgatgggt attgcgaaag cttctcttga agctgctatc cgttatttgg
      541 cagctgagtt ctcgcctaaa ggaattcggg tcaatggaat ctcagctggg gcaatcaaaa
      601 cattagctgt cacaggtgtt aaagactatc aaaaactgat ccagttatct gaatctcgta
      661 cgcctgatca gcaaggtgtg acgatcgaag aagtcggtaa tgcttgtgct ttcttaatca
      721 gcccactttc cagcggaatc atcggtgata ttatctacgt tgacaaaggt gtccatcttt
      781 cataaacttt tgttgcattc tttaaagtaa aacatataaa aatcaggaaa aaacttttta
      841 aagtctcttc ctgatttttt ctttttacgt ttttttcaac tcattacttt gaaattattc
      901 cacgacttca atccagcctt ctggtgcttc tatgtctcca aattgaatac ctgtcaattc
      961 atcatacaac cgttttgtta ctggtcctac ttccgtttca ctataaaata catgaagatt
     1021 gttattgtga taaatcccac cgattggaga aataacagcc gctgtaccac atgcaccagc
     1081 ttctgcaaat tgatctagat catcaatgaa aatatctcct tcaattgctt gcagtcctag
     1141 tcgttcttca gctaactgca acaaggaata tttcgtgata cttggtaaga tagagggcga
     1201 ttgcggagtg atgaattttc cgtcttttgt aattccaaag aaatttgctg cacctacttc
     1261 ttcgattttc gtatgtgtgg ctggatcaag ataaacacaa tctgagtaat ccagttcttt
     1321 tgcttctgtt cctggaagta aactcgctgc gtaatttccg ccgacttttg ctgcaccagt
     1381 ccccttagga gcagcacgat cgtaatcaga tacaataaaa ttcgttggtt ttagtccacc
     1441 tttgaaatac gctcctactg gcgtacagaa gacgacaaat aaatattctg gagctggatg
     1501 gacaccaatg ttttcaccga ccccaatcag taaagggcgc aagtataatg tagcgcctgt
     1561 tccatagggc ggcacaaaat cttcatttgc ttttaccact tgcttgacag catcaacgaa
     1621 catcttttca gggaccattg gcatcagcaa tcgtctagca ctttgattca tccgtcgact
     1681 attttgttca ggtcgaaaga gattgacctt gccgtctttt cgacgataag cttttagacc
     1741 ttcaaaacat tgctgaccat aatgaagcgc tggagagcct tcatgaatcg tcaggtaatt
     1801 atcttctgtc aattctcctt cttcccattg tccgtctttc catttggcaa taaaacgcca
     1861 aggagttttg atataagaaa aacctagatt gttccaatcg atttgcatat acttaccacc
     1921 ttcgttattt tctccacatt ttatcattat ccatattatt tgtcatttta ttttcagctt
     1981 ttttccttca ttatagttat ttttgctatt tccttttttg aaaacttctt ggtacaatac
     2041 agaaagaatc aaattttaga aaagaggatt ttatgttttc attattagca actgccggta
     2101 cgacagactc ttctggcgag acattgacag atcaagctgt cagcaaagta aatgcgtttc
     2161 agcggttttg ggacgggatt gattgggacg ccattttggc tgcctttatc caaaaaagtt
     2221 tatatctgat ttttttgatc gtcctttttt ggattcttca ccgtgtggga aaatatctga
     2281 ttgataagtc ttttaatcag tattctaaaa aagccattct aaacgagagc cgcttaaaga
     2341 ctttacatag tcttttaaat actatttttc attataccct tggtttcttt tttatctatg
     2401 caattttatc tgctatcggt gtgccgatcg gatcactttt ggctggtgcc ggaattgcag
     2461 gtgtggcaat cggtcttggt gcccaagggt tcatgagtga tgttattacc ggatttttta
     2521 ttatcatgga acaacagatg gatgttgggg actacatcaa gctagcaaac ttaacgattg
     2581 aaggaaccgt cgtatctgtt ggtatccgta cccttcagct gaaagcaacg gatggtactg
     2641 tccatttcat cccgaatcga aacatcacca cgatcagcaa tctctctcgt gcaaatatgc
     2701 aagtactttt agatattcgg attgttcctg aagaaggata tgacaaaatc tatgagatca
     2761 ttgatcgagt aaatcgtaca ttagctgaaa aataccaaga agagctccaa actgaacctt
     2821 ctatctttgg attagtggat cttggaaaca gcaattttgc gattcgtacg gtctgctacg
     2881 cattgaacgg aaaacaatat gccttaaaag aagagttctt gagctcctat gtcaaagaat
     2941 tgacagcttc tggtttcacg attccgaaca gtccaattgc tgtcggaaaa taatttggac
     3001 aaacgatgca acgaatctca ctaaaaagag cgtgggacaa aagtaaagaa cacttttgtc
     3061 ccacgctcta aacacgtata aacggcggaa gcagaagcaa ctcct
//
LOCUS       DAZKCC010000158         3071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000158 DAZKCC010000000
VERSION     DAZKCC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.166"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..1102
                     /locus_tag="ACG8VH_002951"
     CDS             287..1102
                     /locus_tag="ACG8VH_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111434.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1228..1485
                     /locus_tag="ACG8VH_002952"
     CDS             1228..1485
                     /locus_tag="ACG8VH_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111435.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1737..2309)
                     /locus_tag="ACG8VH_002953"
     CDS             complement(1737..2309)
                     /locus_tag="ACG8VH_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111436.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2325..2930)
                     /gene="fic"
                     /locus_tag="ACG8VH_002954"
     CDS             complement(2325..2930)
                     /gene="fic"
                     /locus_tag="ACG8VH_002954"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFP9111437.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1018 a          539 c          473 g         1041 t
ORIGIN      
        1 aaataagaaa ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt
       61 ttcttaatag ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt
      121 tattaatcgt agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat
      181 tactatctag tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta
      241 aaaaatactg tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc
      301 aggactttct aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg
      361 gagacagttc agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa
      421 atatttaccg aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa
      481 tgaaggcggg gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac
      541 taaaactata aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga
      601 cgataaccat ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc
      661 ccttgatagt tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt
      721 ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt
      781 tccttataac acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa
      841 atttatcttg gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa
      901 gatttatgac gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa
      961 taatctaaac aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc
     1021 tgaaaaggac tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa
     1081 agaaaacatt actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac
     1141 acttgctatt acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag
     1201 tatcttaatt tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac
     1261 ttgaatgcca ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata
     1321 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
     1381 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
     1441 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
     1501 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
     1561 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
     1621 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
     1681 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1741 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1801 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1861 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1921 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1981 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     2041 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     2101 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     2161 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     2221 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     2281 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     2341 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     2401 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     2461 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     2521 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     2581 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     2641 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     2701 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2761 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2821 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2881 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2941 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     3001 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     3061 gcacagtcta g
//
LOCUS       DAZKCC010000159         3038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000159 DAZKCC010000000
VERSION     DAZKCC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.167"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8VH_002955"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8VH_002955"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9111438.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG8VH_002956"
     CDS             complement(1034..1954)
                     /locus_tag="ACG8VH_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9111439.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG8VH_002957"
     CDS             2067..2819
                     /locus_tag="ACG8VH_002957"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9111440.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DAZKCC010000160         3012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000160 DAZKCC010000000
VERSION     DAZKCC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.168"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8VH_002958"
     CDS             <1..189
                     /locus_tag="ACG8VH_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9111441.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="ACG8VH_002959"
     CDS             complement(275..1369)
                     /locus_tag="ACG8VH_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP9111442.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="ACG8VH_002960"
     CDS             1558..2319
                     /locus_tag="ACG8VH_002960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9111443.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACG8VH_002961"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACG8VH_002961"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9111444.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          973 a          589 c          501 g          949 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aa
//
LOCUS       DAZKCC010000161         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000161 DAZKCC010000000
VERSION     DAZKCC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.169"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG8VH_002962"
     CDS             85..981
                     /locus_tag="ACG8VH_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9111445.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG8VH_002963"
     CDS             1073..1288
                     /locus_tag="ACG8VH_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9111446.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG8VH_002964"
     CDS             1306..1578
                     /locus_tag="ACG8VH_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9111447.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG8VH_002965"
     CDS             1580..2443
                     /locus_tag="ACG8VH_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9111448.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKCC010000162         2736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000162 DAZKCC010000000
VERSION     DAZKCC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.171"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..523
                     /locus_tag="ACG8VH_002966"
     CDS             77..523
                     /locus_tag="ACG8VH_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9111449.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            796..2148
                     /locus_tag="ACG8VH_002967"
     CDS             796..2148
                     /locus_tag="ACG8VH_002967"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP9111450.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2319..2606
                     /locus_tag="ACG8VH_002968"
     CDS             2319..2606
                     /locus_tag="ACG8VH_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9111451.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          960 a          449 c          544 g          783 t
ORIGIN      
        1 tttcttttca tgttaagtgg attttaaaat gtaaaaatag tatgataaga cgagaataac
       61 aacgtggggt gtttacatgg aaaataagtt agtcgaagag gttatcgaaa agttaaaaaa
      121 agagaacatt cgaatcacac ctcaacggta cgcggtttta gaatatttga ttgaacatca
      181 ttcccatcca acggctgatg agatttataa agcacttgaa catcggtttc caaatatgag
      241 tgttgcgact gtttacaata accttcgctt atttacttcg attggatttg ttcaagaaat
      301 gaagtacggc gatgcttcta gccgatttga tttcagttca aagcgtcatt atcatgtaat
      361 ttgtgaaaac tgcggtaaga ttgttgattt tcattaccca gggcttgaag atatagaaat
      421 ggctgcaagc agactgactg attttgagat taatgaacat cggttggaat tgtatggact
      481 ctgtccggat tgtaaagagt cagcaaaaaa ggcgcaaatg taaaaacagt ttctacaagt
      541 gttataccta aaaaaagcta gctgtgtaaa aaaaacgcta gcttttttat ttttttgtct
      601 tttattcaca atcttagata taaaagtgaa tattattaaa ataagaatct tgtagatatt
      661 gggttcttat gcttgaaaag gaatgaaact acaaacaaac ctttgtgaat tcacaagcat
      721 taaactgttg tatccgtttt atttctgtta tagtagtttt gtcgaaggac aaaatgttaa
      781 tcggagggac cataaatgaa agtagtagta attggctgta ctcatgcagg tactgcagct
      841 gtaaaaagta ttttaaaaaa tcatccagaa gcagaagtga cagtatatga aagaaatgac
      901 aatatttctt tcttatcttg cggtatcgca ctttatgtcg gcggagtagt aaaggaccca
      961 gcaggcttgt tctactcaaa tcctgaagaa ttatcttcat taggagcaac agttaaaatg
     1021 gaacacgatg taacagacgt cgatacagaa aataaaacag taacggcaaa agatctgaaa
     1081 acaggagaaa cagaaacggt ttcttatgat aaattagtga tgacaactgg ttcatggcct
     1141 attatcccac cgattagtgg aatcgatgcg aaaaatgtac tcctatgcaa aaactacaat
     1201 caagcaaatg aaattattgc acaagcgaaa gaagcaaaac gtgtagtaat tgttggtggt
     1261 ggatatatcg gtattgaatt agtagaagct tttgtcgaat ctggaaaaca agtgacattg
     1321 attgatggat tagatcgcat cttgaataaa tatctagata aaccattcac agatatctta
     1381 gaaaaagaat tgacagaccg cggtgttact cttgcgctag gcgaaaatgt tactgaattt
     1441 atcacagatg aagaaggaaa agtgaaacaa gtagctacac caacagatac atttgataca
     1501 gatatggtca ttttatgtgt gggcttccgt ccaaatacaa aactgatcga aggaaaagta
     1561 gatacactgc caaacggggc aatcaaagtg aatgaataca tgcagacaag taatccagac
     1621 atttttgcag ctggagactc tgcagtagtc aactacaatc caagtggcac gcaaaactat
     1681 attcctttgg caacaaatgc cgttcgtcaa ggtttgcttg tgggaaataa cttgacagat
     1741 cataaaatgg cgtatcgtgg tactcaagga acttcaggac tttacctatt tggctggacg
     1801 atcggttcga caggtgtgac caaagaaagc gcaactttga acgatctaga agtccaagcg
     1861 actgtatttg aagataatta tcgtccagag ttcatgccaa ctactgaaaa agtaatgatg
     1921 gagctagtct atgaaaaagg aacgaatcgt atcgtaggtg ctcagttcat gtccaaatac
     1981 gacattacac aatcagcaaa taccatgtct ttagctgttc aaaataaaat gactgtagaa
     2041 gatctcgcac tttcagattt cttcttccaa ccacattttg accgcccatg gaattacttg
     2101 aacctacttg cacaagcagc gctaggcgaa atcgaaactg cacaataaag aaaaagggtt
     2161 gtgacacaat tttgtcacga ccctctgtct ttttttgtga aaagtacaat tagttgtctt
     2221 tcttcgaatt ttttgtcaca ataagcagca aagataaagc tgttacataa aagaacaagc
     2281 agctttattt gattttgaaa agaaaggtga gaaagaagat gaataaagaa tattttgtcc
     2341 aacaagatgc atttttagaa gattataaaa gtgcttcgaa gaaattaacg atcaatattg
     2401 atgagttggc agatcaagta ctctctcagt tgaatgaaac gcaagaggaa tattacacga
     2461 tgccagcaaa tgacgccaaa gataatgttt ctcatcgttt tcctttcagt agacgcatct
     2521 actcagatga aaatgacggc acattgaata cagagtacac atatgaaggt cagccatatg
     2581 tgctagaaac tgatgatgat ctttaaagac agtttttgaa aaggccgctc atccttaagt
     2641 gatgacaaaa aatcatcaca attaccgatg actgtctctt tactatcttt taatcagaaa
     2701 aaggggttat gacaagcttt gtcaacccct tttttt
//
LOCUS       DAZKCC010000163         2701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000163 DAZKCC010000000
VERSION     DAZKCC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.172"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            271..657
                     /locus_tag="ACG8VH_002969"
     CDS             271..657
                     /locus_tag="ACG8VH_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9111452.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            751..942
                     /locus_tag="ACG8VH_002970"
     CDS             751..942
                     /locus_tag="ACG8VH_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111453.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1456..1788
                     /locus_tag="ACG8VH_002971"
     CDS             1456..1788
                     /locus_tag="ACG8VH_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111454.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1698..1892
                     /locus_tag="ACG8VH_002972"
     CDS             1698..1892
                     /locus_tag="ACG8VH_002972"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9111455.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            1931..2077
                     /locus_tag="ACG8VH_002973"
     CDS             1931..2077
                     /locus_tag="ACG8VH_002973"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111456.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            2084..2239
                     /locus_tag="ACG8VH_002974"
     CDS             2084..2239
                     /locus_tag="ACG8VH_002974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP9111457.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2515..>2701
                     /locus_tag="ACG8VH_002975"
     CDS             2515..>2701
                     /locus_tag="ACG8VH_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9111458.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT          976 a          450 c          446 g          829 t
ORIGIN      
        1 aaaagattta ttatacgtaa aaagctcaag acattttctt agccataagg acatgtttga
       61 tatttaagaa gatatatgaa ttttcttttc taagtataaa gataactatc cattatcaac
      121 taaaaaaaca tttttgaaaa taaacatcag cagtcctatc aatgcatgtg agcttataga
      181 agttattcgc agcgcagcat aattgcatta gcttcttttc gtgttttttg cctcttttcc
      241 ctttaaatct taatggagga ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc
      301 cagttgattc gattctttga aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa
      361 caagaagaaa ttcaaaaaca tgaacaactt gatcaactcc caccatattt gtttactaaa
      421 gatactataa aatttaaaca accgattaat gttttaaatg tgactctcct tgttgaagat
      481 ataaaattag caaaaatcct tcctttgttg aaagaacctg aacaaatttt tcttatcgaa
      541 aaatttatct ttgataaaac tgataaagaa attggaaagg ttctggggat tacaagacag
      601 ggtgcgacaa atttaaaaca tcgactttac aaaaaaattc aaaaaatgct ggtttaatat
      661 ctgcataata actaattttg tatgcttctt tgctcgttct cctccttccc aaagaaatag
      721 attataagtc catgggaaga aggtagattt atgaattctt taaaagaaga tttcatttta
      781 gcaaaggctg gaaatgaaga agctgttgag gcaattctca aaagatttag ctctttgatt
      841 cataagcaat cctggagaac tggaaaatat gatcaagatt gttatcaaga atgtatgctt
      901 gcaatatatc tagctatctc gaaattcgaa attaaagaat aactcaacta agggagtctg
      961 tgacataagt aaaaagatag tttcaaattt cgaacaataa atttgaaact atcttcttta
     1021 taattataaa gtatcgattt tacagcgttc tataatgatt catagaatct aaaacgaatg
     1081 attttaatta tcaacgtttt ttaattcgtt tttagcttaa aaataacttt tgtcacagtc
     1141 tcactcctaa aattacttta tttacgtatg aagtgggcag gaggtcgaat ccagaactgg
     1201 gctatggtac ttcaacgagg acttctcctc tagaattgaa aagcatgcga tttaccttcc
     1261 gtaacgacgg tagatttatt caactatgtg tccaatcaaa atttgtctga tttcaaaaat
     1321 agtcaagaga attttggttg ttaaaaactc ttatctatca acttccacag tttagttgac
     1381 aaaccctgat actaagttgg gctatgatac acttttcttg actatcagtt acgttaccgg
     1441 aaggaatgaa ctctattgag aaaaacatat gatcgtaaat ttaaactaaa gatttcacag
     1501 gatatccttg agaaaaaagt aactaccaag aaaattgctg aagaatacaa tatctctcgt
     1561 ccaacaattt cccgatgggt ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga
     1621 caaggaaaaa gattacctga taaagcagat ttctacattc ttgaacagga aaataaacga
     1681 ctaaaagagg aagacgagtg ttctacgaac acaaagatcg atacggatct ccagggatca
     1741 ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc
     1801 gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa atcttctcat ggtagagaaa
     1861 aaacaatcaa agaaatggta aaaaaatgtt gaatcgataa tttgaacaga accaaattga
     1921 tgctgtttgg gtgatggata ttacttacat tccatgtaat gacggtcgcc tctatttgtc
     1981 cacctatatt gacttagcaa ctcgtattct acgttactat atggtagatt cacacatgaa
     2041 aaaagagatt gtcgtccgtc cattacaaat ttattaagga gaattgccta cggtaattca
     2101 ttccgataga ggtagccagc atcggtcttt tatttatcaa gagctgttga acgaccacca
     2161 catcacacat tcagtgagcc aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt
     2221 tcatcgatca atcaaatgag aattaatcga ctccaacaaa tataaaagta aaatagagat
     2281 gaaggtgtta atccaagatt atttagaaga ctactaccca aacaaaagaa tccatacaaa
     2341 atttatgatg actccttgac aatttgaagc gaagaaaata agggatctat aatatctagt
     2401 gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc aaacagacac
     2461 cttaaaatcc tttagaataa agtcgacgaa aaccattcaa aggagtgttt caagatgtcc
     2521 tattcagagt ttaccaaaga gttactagat attctagacc taaatcttat ctttcatgaa
     2581 gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga tggcacattg
     2641 acctatcaac cggaggaatg ctttcatcgc cactatcaaa atgaacaaac gatcattaaa
     2701 t
//
LOCUS       DAZKCC010000164         2639 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000164 DAZKCC010000000
VERSION     DAZKCC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.173"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8VH_002976"
     CDS             137..748
                     /locus_tag="ACG8VH_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9111459.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8VH_002977"
     CDS             821..1750
                     /locus_tag="ACG8VH_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFP9111460.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2639
                     /locus_tag="ACG8VH_002978"
                     /pseudo
     CDS             1887..2639
                     /locus_tag="ACG8VH_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
BASE COUNT          923 a          368 c          490 g          858 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaa
//
LOCUS       DAZKCC010000165         2619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000165 DAZKCC010000000
VERSION     DAZKCC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.174"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..317)
                     /locus_tag="ACG8VH_002979"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACG8VH_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(349..672)
                     /locus_tag="ACG8VH_002980"
     CDS             complement(349..672)
                     /locus_tag="ACG8VH_002980"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9111461.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            1134..2516
                     /locus_tag="ACG8VH_002981"
                     /pseudo
     CDS             1134..2516
                     /locus_tag="ACG8VH_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          940 a          456 c          433 g          790 t
ORIGIN      
        1 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
       61 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
      121 tttttcttct tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc
      181 gtagtatgac aaacatttat tccacgatca tatcacaatt cctgaacttc acgatagctt
      241 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
      301 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
      361 agattttttg gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg
      421 gttttcatct aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac
      481 atctaataca aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc
      541 atcatcataa tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga
      601 tctgttttgg tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg
      661 aaaactgtcc attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt
      721 acaacgaata ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat
      781 ggtgttaatg acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag
      841 attaaaattt cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa
      901 tcagacttga aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc
      961 aaaatcttac ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac
     1021 tagctacatc acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa
     1081 gggggtgcgg acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt
     1141 cccacaaaga gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg
     1201 caaccattcg agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt
     1261 atttggaatc cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac
     1321 aaaaacgaat agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc
     1381 gcatgcttgg ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag
     1441 gagctagaaa actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt
     1501 tgaaaaaatt ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta
     1561 gtagagttac tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta
     1621 tgactttaaa aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac
     1681 aaaaacaagt acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc
     1741 taaaaaaaga aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga
     1801 ttgatgcctt acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta
     1861 aaagcagcta tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac
     1921 gtcaaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt
     1981 caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg
     2041 actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg
     2101 gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat
     2161 gaaaaatggt taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca
     2221 ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact
     2281 tgcttcactc tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct
     2341 ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc
     2401 agtttataaa caaattggat gattatattc attggtataa taatcaacga attaagttat
     2461 ctttaggaag gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag
     2521 gtccaaaaaa aatatccgca cccccttttt ttgtaaaaaa ctattgcaca atacaagtag
     2581 cctacggcta tcgaaatttc cataactttc gagcaagaa
//
LOCUS       DAZKCC010000166         2423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000166 DAZKCC010000000
VERSION     DAZKCC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.175"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..2276
                     /locus_tag="ACG8VH_002982"
     CDS             123..2276
                     /locus_tag="ACG8VH_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP9111462.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          828 a          426 c          536 g          633 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa ggttgtgaca aaa
//
LOCUS       DAZKCC010000167         2418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000167 DAZKCC010000000
VERSION     DAZKCC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.176"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG8VH_002983"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG8VH_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG8VH_002984"
     CDS             1320..1778
                     /locus_tag="ACG8VH_002984"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9111463.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG8VH_002985"
     CDS             1937..2407
                     /locus_tag="ACG8VH_002985"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP9111464.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          699 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaa
//
LOCUS       DAZKCC010000168         2399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000168 DAZKCC010000000
VERSION     DAZKCC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.177"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..2070
                     /locus_tag="ACG8VH_002986"
     CDS             208..2070
                     /locus_tag="ACG8VH_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9111465.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2399
                     /locus_tag="ACG8VH_002987"
     CDS             2070..>2399
                     /locus_tag="ACG8VH_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9111466.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataat
//
LOCUS       DAZKCC010000169         2271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000169 DAZKCC010000000
VERSION     DAZKCC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.178"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..446
                     /locus_tag="ACG8VH_002988"
     CDS             192..446
                     /locus_tag="ACG8VH_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9111467.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="ACG8VH_002989"
     CDS             447..764
                     /locus_tag="ACG8VH_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9111468.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="ACG8VH_002990"
     CDS             1323..2258
                     /locus_tag="ACG8VH_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111469.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          430 g          711 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga g
//
LOCUS       DAZKCC010000170         2199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000170 DAZKCC010000000
VERSION     DAZKCC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.179"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..1090)
                     /gene="cysK"
                     /locus_tag="ACG8VH_002991"
     CDS             complement(164..1090)
                     /gene="cysK"
                     /locus_tag="ACG8VH_002991"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9111470.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1230..2117)
                     /locus_tag="ACG8VH_002992"
     CDS             complement(1230..2117)
                     /locus_tag="ACG8VH_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9111471.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          615 a          486 c          365 g          733 t
ORIGIN      
        1 gaaaattcaa ttttattgta ttttaatgaa aaaagacgta aaacagtcgc gttcactatt
       61 ctacgtctct attcaaataa tttttctcat cataaatttt tgtgtgttct tttcatcttt
      121 aaaagttttt tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa
      181 gggctgtaga taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt
      241 ttcctaattc ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta
      301 ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa
      361 tagaaaggac tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga
      421 ttccttggat tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt
      481 ctaccccgat taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga
      541 tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt
      601 gatgtatctc aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt
      661 gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag
      721 aaataccatc cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac
      781 gtctttcgat actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc
      841 ctaccatcga caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg
      901 gtttcagtag accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta
      961 cacttcctcc tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta
     1021 cgattttatt taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga
     1081 tagctgtcat cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg
     1141 ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat
     1201 atggtaattt ctttgccttt ttatctaaat caaacgttta gttcttgacc aactaataaa
     1261 gaataactgg acatagacat gccatttttc gccattagat catcaacaga catgccaaat
     1321 ttttgtgcaa tatcccatag cgtatcacct gacacgactg tatatgtctg accattattt
     1381 gtatttacat ctgttgttga agatgatgac gttgaagtcg tcgtagtagt cgttgttgtt
     1441 gtacttgtgg ttgttgtagc cgaacctgtt gatggcgtat caaaacgggt taaattataa
     1501 gtattaatca atgagttcaa tttggcccca taatttggat cggtagcata acgacctgtc
     1561 aagtacgctg ttgcatcata aaaacttgtt gtattgcttt tccacacgcc agcataatga
     1621 taatcgcccg tggaaaaact ggttgaacga agaacattcg catggtcttg gaaggattcc
     1681 caataagaag aatattttcg gaatggctca ttcatcgtta cccattgtcc gtttacatat
     1741 tctttggttg ccatataaac gacctgtccg ttatagcttc ctttcacacc gaatagatta
     1801 taatttggcg cactagctaa tccggaagtg ccatatgcac tttccaacaa cgcttgagcg
     1861 atcatgactg aagcatacaa gtcattactt gcagcaactt ctgacgcaga ccagccgatt
     1921 tgatcgataa acgcttgagg atcagcgact gtttgagatc cttgttcatc tgcatgaaca
     1981 tgttgcggta ccgctgtaac catcatcggc ccaactaaaa gactcgttcc aattaaagga
     2041 gcaatcgctt ttttattgat caccattttt cttttaggtc tatggtgccg ggcactgcgg
     2101 gtcttcagct cttgcattca aattcctcct caaaataaaa aaacttaata aaaagttctc
     2161 ttcttaaaac aaacttaatt aattaaatta tacttttaa
//
LOCUS       DAZKCC010000171         2196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000171 DAZKCC010000000
VERSION     DAZKCC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.180"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..364)
                     /locus_tag="ACG8VH_002993"
     CDS             complement(65..364)
                     /locus_tag="ACG8VH_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9111472.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            715..1347
                     /locus_tag="ACG8VH_002994"
     CDS             715..1347
                     /locus_tag="ACG8VH_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9111473.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1470..2108
                     /locus_tag="ACG8VH_002995"
     CDS             1470..2108
                     /locus_tag="ACG8VH_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9111474.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          723 a          354 c          324 g          795 t
ORIGIN      
        1 tgaacctagc ctgcattatg agattgacta tactttttaa ttcattgaga tgaataactc
       61 gtttttataa atgcccaaaa gtaatcggtg ccgaagccat tcctcgacct aattcttttt
      121 caaattttcc tattgttgat atctccatat aaaacggtga tactggttta gacaattctc
      181 ccctgattgc taacggacgt aaactacgtt ccaaattatt tatgacttta ggataatact
      241 gacctttttc taagcctgtc tttgcgtcta tcaaaagtat acgttccttt tctttgatct
      301 ctggatttaa aacgagatca tataatttta gtaccatgtt ttctatttct tcacttttag
      361 acatcacgat ttcctcccaa aaatatattt cttcccacta cattctataa acaaagtata
      421 cgctacatct agattttttt aaatcattac tggattgtta aaacctttca atacactgtt
      481 atggcaacga tttttatttt aaaaacattc taaattaaat cagtaaaaat ttttattcta
      541 agatttttat tagaattacg cctaatttgt gtctctcagg gcaattctaa gaacgtttca
      601 agagatagcg acactatcgt tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg
      661 taagttggtt tagcttttgt taatatgact ttatgaaaat ggaggtaatt aaatatgatt
      721 tttcctaaag ttaatgaagc aagcttgaat aaaggatcct tttttcatat ctatgaaaac
      781 gaagattttg aactgtataa tatcctttca caaaaagagg tcatcgaaaa ccaaataatc
      841 tgccatccaa ttatttacaa atctattttc aagtatgcat tttttgcttc caatgatttt
      901 caaagaatgg agattaccga ctgtatattc gaaaactgtg atttttcagg ctgtatattg
      961 acaaaaagca tgttgcatcg cgtgaaattt gtcaattgta aattaacagg tactaaattg
     1021 atggaaagtt atattgggaa cacgttattc gagaattgta aaatggacta tgtaaattta
     1081 tctggttcaa acataaaaga aagtaccttt gagcactctg tcttatcaag cgcagatttt
     1141 gtagactgtt ccctcactaa aataatgttt tctactaatg atttagaaaa tgctagcttt
     1201 tttgatactg atttaaaaaa tatcgattta agtagcaact cctttgaaat gattgaagta
     1261 cgctcggaac atctaaaaaa ttgtacgctt aatcagttgc aagcacttgg ttttgctaga
     1321 aaatttttac aaataaaagt agtataatca aaatgaatat ataagataaa tttttagcaa
     1381 tacaaatatc cataaaaaaa cattgcctat tttaactctt catgtatact gactctatct
     1441 tcaaaaactg aatagtagga gttattatca tgaataacat tttaggaaaa aaaatagaac
     1501 ttaaaaaagg gatattgggc actattgtcg ttgtactcgg tttgttaggg ttgctatttg
     1561 cgccatccat tattagcatt ttttctttaa ctatcactgg atttatagca tttacacttg
     1621 tttttggtaa aaaagatacg aaaaaaatgt tctcgaaacc tgtcgctcct gtcaaaaata
     1681 tagccaaata tttttttatc aatgtagtaa tcagtgccgc agtctcggta tttttgcaac
     1741 aaattttgaa atggggttta acaggcaatc caataaatga ggctttcagc ccgttattat
     1801 ttattatttt gcctatcatg atacttggag aagaattgtt ctcagtgtat tttctcgcta
     1861 ttttttctag taagttcagc attcctgtcg ctagtttctt gtcagcaatc atctttggat
     1921 ttatccatta cagtacttat gataatggaa atattttaca cactgtagca cacatccttt
     1981 tgatacaagg ggtggcaaga ttgttattca accaagctgc cattaaaagc aactcgatca
     2041 ttacaagttg ggcagttcat gtgatatttg atttcacagc tatattgcta gtcgttttat
     2101 tctcctaaat ttttatagaa ataatacatg ctctattgct aagaaagcat actttatttc
     2161 ctttaaacaa aaagacagat gtatcaatgg attaaa
//
LOCUS       DAZKCC010000172         2127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000172 DAZKCC010000000
VERSION     DAZKCC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.181"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..526
                     /locus_tag="ACG8VH_002996"
     CDS             <1..526
                     /locus_tag="ACG8VH_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111475.1"
                     /translation="FKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLA
                     MAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRR
                     RCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFA
                     REDEDNPKTYDILA"
     gene            complement(587..952)
                     /locus_tag="ACG8VH_002997"
     CDS             complement(587..952)
                     /locus_tag="ACG8VH_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP9111476.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1028..1273)
                     /locus_tag="ACG8VH_002998"
     CDS             complement(1028..1273)
                     /locus_tag="ACG8VH_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111477.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1343..1597)
                     /locus_tag="ACG8VH_002999"
     CDS             complement(1343..1597)
                     /locus_tag="ACG8VH_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111478.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1761..2084)
                     /locus_tag="ACG8VH_003000"
     CDS             complement(1761..2084)
                     /locus_tag="ACG8VH_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111479.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          795 a          361 c          372 g          599 t
ORIGIN      
        1 ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat
       61 tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt tactatcaat
      121 attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac tgatcacaaa
      181 atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg actacagagg
      241 cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc gatgtagtaa
      301 tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct
      361 gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg
      421 ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt ttagttttgc
      481 acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta agatggttaa
      541 agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag gtaagataaa
      601 ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg tgttccggat
      661 aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat gatttgccac
      721 gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc atttcatcca
      781 tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca taatccacag
      841 tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga tgttgtccta
      901 aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc atagaaatgt
      961 ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc accaaggctg
     1021 gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa
     1081 tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct tctccttctt
     1141 cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct acttgagata
     1201 aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt aaaatagctt
     1261 cttcgatact catttaatca cctttcatta tagtagaata gttataatga ctatagaaag
     1321 ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa taaagaatga
     1381 cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta tcgggttcaa
     1441 agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc aaaatagcta
     1501 aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc agtgcgaccg
     1561 caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct caacgattca
     1621 tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg aaaataaaag
     1681 aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa gcaggaactg
     1741 ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc taagtaattc
     1801 aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat gatcccatag
     1861 cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct ttttccttta
     1921 tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat cattccaata
     1981 aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc cgcaactaaa
     2041 tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa taaataaaag
     2101 aataacattt tttctaaaat aagcaaa
//
LOCUS       DAZKCC010000173         2052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000173 DAZKCC010000000
VERSION     DAZKCC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.182"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..164
                     /locus_tag="ACG8VH_003001"
     CDS             <1..164
                     /locus_tag="ACG8VH_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9111480.1"
                     /translation="ISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHAN
                     EVLKIAEEK"
     gene            165..917
                     /locus_tag="ACG8VH_003002"
     CDS             165..917
                     /locus_tag="ACG8VH_003002"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP9111481.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            922..1869
                     /locus_tag="ACG8VH_003003"
     CDS             922..1869
                     /locus_tag="ACG8VH_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9111482.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          715 a          274 c          411 g          652 t
ORIGIN      
        1 aaataagttt tgaagaaaac attgttaaag aaatgcctga atctgctaat gatatgtatg
       61 agaaggagtt gaaatatttc tttgaaaacg tagtaacagg caaagaaaac tggaatgatt
      121 tatatcatgc aaacgaagta ttgaaaatag ctgaggagaa ataaatggaa attttattta
      181 ctatttgtgg tagagctggg tctaaggggt ttaaaaataa aaatttgaaa tatttggcag
      241 gaaagcccct agtccatttc gctttagcaa tgatagatat ctatcaacaa aaacatcctg
      301 aacataattg cacgacttgt ttgaatacgg acagtaatga gttagtagaa ttgacgacta
      361 agttaaatcc gtctgtgaaa attgtagaac gtaaacctga attagctaca gatactgtag
      421 ggaaaattga agtgatacgt gattcattct tacaactaga aagagaagat aatatatttg
      481 atttaattat agatttagat ttgacttcac ctttacgtga agtttctaat cttgaagaat
      541 taatagatga acatctaaaa aatttagata aagacgtcta cttttcagtt gtttctgcaa
      601 gacgtaatcc ctattttaat atggtaaaaa gaattgaaaa tgaagttact atagttaata
      661 aatcaagata cacatctagg caacagacac ctgaagtttt tgaaatgaat gcctcaatgt
      721 attcttttaa acctgaattt ctaaaaaaaa gtaattatat ttttgatggg caatgtggga
      781 taataaaaat gagagattat ctaattttag atattgatag tgaagaagat tttaaatggt
      841 tagaattttt gtttcctaaa tttttagaag attcttcgga gcttcaagat atctataatt
      901 atttatatcg gaggtaaaag gatgagctgt tatcttatca ccggtggggc tggcttcatt
      961 ggttctacct tagccaatca tttaggagaa gaacatgtgg taatcgtcgt cgatgactta
     1021 tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata tcacttttat tgagggagat
     1081 gtcgctgaga aatccttgat ggaagagatc atgaaagcta ataaatttga ttatatcttt
     1141 catttagcag cagttgctag tgtggcagat tcagttgccc gaccagtaga aacacatcgt
     1201 gtgaattttg aaagtgtatt aatgatttta gaattgatcc gcaagtatca acctgattta
     1261 aaacggatcg tgtttagttc ttcagcggct gtttatgggg atgaaccgac tttaccaaaa
     1321 aaagaagagt ccgtgattcg cccattgacg ccttatgcgg ttgataaatt cgctgcagaa
     1381 caatatgtgc tagattattg tcatttatat gatgtacccg gtagcgcagt tcgttttttc
     1441 aatgtctatg gtccaaatca aaatccgaat tctccataca gtggtgtgat ttctattctc
     1501 gtagatcgct ataagaaaca aatcgtagga gaagcaactt catttacatt atttggtgat
     1561 ggaagccagt ctagagactt tgtttatata gatgatgtaa tccaagcatt acttttagtt
     1621 gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca
     1681 cttttggaat tgattcatgc aatcaatgaa attctcggtg tagaattgac acttgaatac
     1741 aaagaagagc gttcgggtga tatccatgac tctctagcag atattactaa gatccagtca
     1801 attggctatc aaccgaaata tggtatttta agtggaatga ctaattattt gaaaactgaa
     1861 atcaattaaa acatactaag ataagattag agggtaaatt caggctcttt gtcaataagg
     1921 actgatgagt tttgtaaaat caaatctggg atagaagggc tctttgtcaa atgatgttgg
     1981 taaagagaaa tcttagaata atttggacag aacagaaaga agaaaacctc tttctgttct
     2041 gtttatttgg tt
//
LOCUS       DAZKCC010000174         2044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000174 DAZKCC010000000
VERSION     DAZKCC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.183"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..424)
                     /locus_tag="ACG8VH_003004"
     CDS             complement(<1..424)
                     /locus_tag="ACG8VH_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP9111483.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="ACG8VH_003005"
     CDS             complement(533..1564)
                     /locus_tag="ACG8VH_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9111484.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          629 a          331 c          353 g          731 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgcttattga
     2041 tgga
//
LOCUS       DAZKCC010000175         2020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000175 DAZKCC010000000
VERSION     DAZKCC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.184"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACG8VH_003006"
     CDS             100..570
                     /locus_tag="ACG8VH_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP9111485.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACG8VH_003007"
     CDS             792..1895
                     /locus_tag="ACG8VH_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP9111486.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          546 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DAZKCC010000176         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000176 DAZKCC010000000
VERSION     DAZKCC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.185"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8VH_003008"
     CDS             <1..378
                     /locus_tag="ACG8VH_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9111487.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8VH_003009"
     CDS             426..1826
                     /locus_tag="ACG8VH_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9111488.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKCC010000177         1967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000177 DAZKCC010000000
VERSION     DAZKCC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.188"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            228..1244
                     /locus_tag="ACG8VH_003010"
     CDS             228..1244
                     /locus_tag="ACG8VH_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9111489.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
     gene            1444..1626
                     /locus_tag="ACG8VH_003011"
                     /pseudo
     CDS             1444..1626
                     /locus_tag="ACG8VH_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          650 a          310 c          455 g          552 t
ORIGIN      
        1 tgctataatc aaagaaaacg agaaaaaaga gcaaaataaa agccgagcag tgcgctaaca
       61 ctctcgacta tttacggaaa ctacttcacc tagctccgta actcaggttc attatacgaa
      121 tatttttagg tttcttcaag ctagagattt ttggatatga atttttaggg ttacgtgaac
      181 tctggaaatt ctttccaaaa gtctcttttt tcgtttggag gacgaaaatg gcaaatgaaa
      241 atcagttgag tgaaattcaa tcaaaaattg aaggtcgagg ggcagaaaaa agaacaacga
      301 taaatcagct cgaaaaactt aaccgtattt ctaacaggcg gttaagtgtg caaggtacga
      361 ccacccccct aaaattaaat tggtcgattg atcgaattac aattgtcgga aaactgaatg
      421 ataccatagc ttattggacg aaaaatggag ttgtttttgt cgatttcgag caattaatgc
      481 gtttgaacga aaatcaggga tatttgaaat cagttgggat aaacggctgg caattattcg
      541 atcaatatgg agaagatatt gcgtatatcg aaatacttaa atttcatgaa ggaaaaggtc
      601 gtattgattt taatccgaat aagataggcc agtttttagc tagttccatg aaaggattca
      661 ttcatgacct atttttagat ccgcattttt cccgggcgga tattgcctgc gatattgtag
      721 atgtgccgga tgactttatt actcagtatc gtattgtaga cccgatttct tttaagccaa
      781 tctacggccg ttctggcaag ctagaaacag cgtactgggg cagtcgtgcc agtgagcgcc
      841 aagtgcgact gtacaacaaa aagctagagc aggaacgaaa aaaagtaatc gtgccaaaag
      901 aaatcgatac gtggtggcgg ttagaaatgc agctaagacg tggcaaagca acggattggc
      961 atgcgatggt tcaggaaagc ttagatagtt ttgcaagccc gcattttttg ccgattgata
     1021 taaaacctat tgataaaata gttattgatg gtttgatagc tgagccttct aattggtcga
     1081 taattgctag acatacaaaa tataaatatc ggaatttgct gaagcaagaa agtcaaaatg
     1141 atgagttaac gaatcattta cgagagactt ttaaggaatc tgcagacgaa ttaaaaaaag
     1201 aattggatac gtggcttttg gggttggatg tgacagagaa gtgataccga ataagaaaag
     1261 tcagatgagt gagagtggat ttgcgtcaat gtttagtgac gtaaattcac gataagcgaa
     1321 tattttgcat gaagcaaaat aaagcgtaaa aggagttgag agcgtgacac gctttcggct
     1381 ccttttttcg ttgtcacttg tgacagcgaa atccctcgga gagcgtttgg aattgtgaca
     1441 gccatgtcac aattaggaaa acgttactct ttgtcaaaga gtaacagaaa ggtttacact
     1501 ctttttatta gcgacagcgt gaaggagggg ttcaccttag ggtttagtat ttcgttcaaa
     1561 aagggaacaa agaatacaag catttctcat aataatcgtg agttaactga tagccaaaaa
     1621 atcaatggtt tgtctggtag aaaataatta gcagatttac aagtgggaaa taaaaagctg
     1681 aaagaagaaa attgcatttc aaactacatt ttctacaata gctctaaaat ggcatatttt
     1741 gcgttctgag cggttttagc gtttatttcg tttagttatc ggcacaatcg ttaaaacacg
     1801 ttagaggagc gaaaatggag cctgtaaggc gactggtagc ggaacgatcg tgttagattg
     1861 tgaaaaccga tagctgaatg aaataaacag cgggtgcgcc ctttccattt cggcaggagg
     1921 aggctcaagg ggttcgggga atgaaattcc ctgaggtctt tttattt
//
LOCUS       DAZKCC010000178         1958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000178 DAZKCC010000000
VERSION     DAZKCC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.189"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG8VH_003012"
     CDS             288..695
                     /locus_tag="ACG8VH_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111490.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG8VH_003013"
     CDS             709..1110
                     /locus_tag="ACG8VH_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111491.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACG8VH_003014"
     CDS             1112..1483
                     /locus_tag="ACG8VH_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111492.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="ACG8VH_003015"
     CDS             complement(1519..1818)
                     /locus_tag="ACG8VH_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9111493.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          721 a          263 c          323 g          651 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcaca tcagtcacga tatcaaacaa
     1621 aaaatatacg tctttcttca ttctagtttt cgcagcaggg attttaaagt agttcttatt
     1681 agaatagtag agattaatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatc
     1741 ccacacctta taaaaatctt tgacaaatct attcgaagga tcaaatttaa accataattg
     1801 cgtctcatta aaaagcataa ccattactcc gatctatttt taaacttagt ctctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaaaat
//
LOCUS       DAZKCC010000179         1896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000179 DAZKCC010000000
VERSION     DAZKCC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.190"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..869
                     /locus_tag="ACG8VH_003016"
     CDS             273..869
                     /locus_tag="ACG8VH_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9111494.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(927..1409)
                     /locus_tag="ACG8VH_003017"
     CDS             complement(927..1409)
                     /locus_tag="ACG8VH_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111495.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1515..1712)
                     /locus_tag="ACG8VH_003018"
     CDS             complement(1515..1712)
                     /locus_tag="ACG8VH_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111496.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          652 a          370 c          283 g          591 t
ORIGIN      
        1 ttttaatggc aatatcataa aaaaaagaag atagagattt aataattata tatcagctgt
       61 ttttttcaaa taagcaaaaa ttcctaaaac acaacaatga aagcgataac taagaaaatc
      121 cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt ttttgtgagc
      181 atatctgttt acttactttt tgtaaggtgc tatacttcat ttcgtaatca attaaaaaaa
      241 tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac actttgaaaa
      301 accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa gaattaacaa
      361 ctttgatcaa agaagcaatt aaagaaagcc caactgcttt caatgctcaa tcaacacgcg
      421 cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa gaagcacttc
      481 gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct ggtttcaaaa
      541 atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt ctacaagaac
      601 aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac ggaatcgcaa
      661 cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg caacattaca
      721 acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac tggaaactac
      781 gttcgcaatt agtattcggt tcaccagaaa caccggctga ggaaaaagaa tacatgaacg
      841 atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca gaggttgtga
      901 caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc aatctctgcc
      961 tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc agaaaatttc
     1021 agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt agaacctttt
     1081 ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt gtcaaagcta
     1141 ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact aataaaaatc
     1201 attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat gagtgtatac
     1261 caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt tcgcacagtc
     1321 cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac aacgaataaa
     1381 aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc ttctccagaa
     1441 aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc acgccgcacg
     1501 tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct tgctttttca
     1561 tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc gcagtttgtc
     1621 atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt gcatgcatat
     1681 tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg ctggaacttt
     1741 tctccacatt accactgcta tttctttgga gatttaactc attgattttt atttacagtt
     1801 aaaaatcaac cttacaggca ttatagcgta ctgttttcgt tttcacaatt aaaacagctg
     1861 ttgtatttag aagaattttt ttcaaaaaat taaaac
//
LOCUS       DAZKCC010000180         1890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000180 DAZKCC010000000
VERSION     DAZKCC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.191"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            385..999
                     /locus_tag="ACG8VH_003019"
     CDS             385..999
                     /locus_tag="ACG8VH_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111497.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1023..1373)
                     /locus_tag="ACG8VH_003020"
     CDS             complement(1023..1373)
                     /locus_tag="ACG8VH_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9111498.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1367..1630)
                     /locus_tag="ACG8VH_003021"
     CDS             complement(1367..1630)
                     /locus_tag="ACG8VH_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFP9111499.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          625 a          328 c          289 g          648 t
ORIGIN      
        1 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
       61 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      121 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      181 tctcttaaat tttcttgatt gacatttatt tacaaaaaat attgccattt aaaaagaaaa
      241 gtgacacata ctatttcctc catcattagt ttttgtctat ataaggtata ccctaactag
      301 acacgaccgc gacattgaaa aatctggttt tctcgtgtat gattagggtg ttttttaaat
      361 agttgcagaa agtaggtaaa aattttgaga gttgcataca tacgtgtctc atccatcgat
      421 caaaatgaac aacggcagat tgaagaaatg aaaaaattcg gtgcagagag aattttcatt
      481 gagaaacaat ctggcgcaac gattactcat cgacttgttt ttcaagaagc actggatttc
      541 gtccgagatc aagatatttt tattgtggaa gctattgatc gacttggaag aaattacgac
      601 gagatcatcg cttcagtcaa ttacttgaag aagaaaaatg tgcagctgat catcacaagt
      661 ctaccgatca tggccgaagc aattggcaat cctctcctgg ataaattcat taaagatttg
      721 atcattcaaa ttttggccat gatcgctgaa caagaacgta ccgaatctaa acgacgtcag
      781 gcacaaggca ttaagattgc aaaagcgaac ggtgtttata aaggacgtcc taagctatac
      841 agtgccaatg caaaagatcc ccaacgtcgt cttgtttata aaaacatcgt tgaggatcta
      901 aaaaaaggtg ttgcgatcgc taagattgca aaagactaca atgttacgag acaaaccgta
      961 tatagaataa aaaaagacag catggtcaat cacgaatagt tgatcatgct gctgtttcta
     1021 ttttatttgc ttactttttg aaaaactgtt ctaactttca tcaataactc ctcaactaaa
     1081 ctatcatgcg ctttttccaa aaaaatacac tctctggctt cataatcaat tgtcgttagt
     1141 tgatctaata atacttttcc tgttgtcttc atttcatatt ctgagtttat atttacatat
     1201 agcggatagt ttctagttgt actagaaatt ggagcaactg taacaacgtt actataatcc
     1261 gcaataatat catgacttaa aacaatataa ggtcttttac ctgtctgttc gtgtccagat
     1321 cgaggtgctg tattcaataa tagaatatcg ccttgtctag gttttctcac catttttcat
     1381 tacctactgc ttcacctaaa ttttgaatcg tcacatgatt tctttcatca gtgtaatttt
     1441 caaataactg ttcaattgtt aattcatttt ctatattagc cgcttcgata attagccgct
     1501 tctgaccgtt tacttcttct tgtattatct tcactttgtc ttctgtatga attccaagat
     1561 attccataat atactttggc aatctaaggc cattactatt tccccatttt tttataggta
     1621 acatttccat agtagacacc tctttctgta tatacatagt ataacataca taagaaaaaa
     1681 cgtatatact taattatcct tttctattaa attagattaa cattccatga atccataaac
     1741 tatgttaaca tttatatgaa aatagtcgaa aggaaatcat aatatgttta agaaaacaaa
     1801 atctttagaa tcaggtaaga aaaaaattga tctaaaaata atctcttctg gatactttaa
     1861 agatttcata gttattgata caaaatggaa
//
LOCUS       DAZKCC010000181         1749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000181 DAZKCC010000000
VERSION     DAZKCC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.192"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..1632
                     /locus_tag="ACG8VH_003022"
     CDS             382..1632
                     /locus_tag="ACG8VH_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9111500.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          577 a          335 c          309 g          528 t
ORIGIN      
        1 tgtctaatag tttttattca aaatgtttct agattataaa atttattgaa taacacattc
       61 ctaacggaat ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatggaaagg
      121 atccaacggg aatttgctat ttcctcctgt agcgtttgtc taccctgttt agtctgttac
      181 ccgaaccact gttatcacta ccaactcatt agctgtttcg ataatggact agactttcaa
      241 atagtagttt ggaaggcagc ttctagctct tttatcaccg tattctatta ggcgaggttg
      301 tggctataca gtgagtttct gggaaatccc acagtatcga agcattttcc aaaatttata
      361 tcgaaaggag gtccttcttc catgaaatta tttgtcggta ttgacgttag ctctgaaaaa
      421 ctagatgttt gttttttaac agacgacaat caattgtcta tcttatctga aatctctgtt
      481 gccaatgaca tcgaaggtgc ttcattcata cgagaaacaa ttcttgaatt caatgacagc
      541 taccactttg atcaaattgt gattggcatg gagtcaacgt ccatgtacag cttccatcct
      601 tccatgtttt ttcatgaaga tgagaaacta aagaagctca acacacttgt aacgattgaa
      661 aatccttttc gggtgaaaca atttagtcgc atgtttgatg agaataaaac tgatcgaaac
      721 gatgcattaa gaatcgctga tttcttgcgt attcaacgat tcacaacttc ccctatcaaa
      781 gaagagaaat acatggcctt acagcgttta acacgtactc gctatcaact tattaagcaa
      841 ttgattcgta caaagcaaca ttttcttgaa aatctaacgt acaaatgtaa tacgcttgct
      901 cgagaaatgc gagacgaatc gacgagtctt tttagtgcaa ccctcatttc actcatgacg
      961 gaagatttca cattggatga acttgctgag cttcctttag aagctttctg tgatttactt
     1021 caagaaaaag gaaaaggccg tttcaagcaa ccagaaaaaa tcgctaaagc aattcaacgt
     1081 gcaattacca tgagttaccg cctaggtgct cttgcacaag aatcaattaa tgttgtttta
     1141 agtgttctcg tacgagaaat acgagcactt gaaaaaaata tcaaagaatt agataaagcc
     1201 atcgaacaag taattgttgt cattccagaa tatcaatgct taaccagtat tccaggcgtt
     1261 ggtaaagtct atgctgccgg tttaatcgct gaaattggtc aaatcgaaag atttgaagat
     1321 cagacaaaat tggcaaaata cgctggatta agttggaaag tgaaccaatc tggaaactat
     1381 cagtcgcaaa atacacctct gacaaaacaa ggaaatcgat attttagata ctacttagtt
     1441 gaagctgcca actctgtaaa aaattatctt cctgaataca aagcttttta tcaaagtaaa
     1501 tataaggagg ttccaaagca tcaacacaaa cgtgcactcg tcctaaccgc aagaaaattt
     1561 acgcgtctgg tggatacgct actacgtaaa caccaactct atacgccacc aaggagtgtg
     1621 atagagaaat aacaatccgt tattgacgca aatccttgaa tcaacccgaa aaaaatttga
     1681 ttttgatcgg gtctagtttc gtgcgcttca aaacatatta ctcaataaag aaaatcttaa
     1741 atttcatct
//
LOCUS       DAZKCC010000182         1731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000182 DAZKCC010000000
VERSION     DAZKCC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.193"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8VH_003023"
     CDS             <1..1412
                     /locus_tag="ACG8VH_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9111501.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>1731
                     /locus_tag="ACG8VH_003024"
     CDS             1546..>1731
                     /locus_tag="ACG8VH_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9111502.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQ"
BASE COUNT          684 a          256 c          370 g          421 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca a
//
LOCUS       DAZKCC010000183         1731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000183 DAZKCC010000000
VERSION     DAZKCC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.194"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8VH_003025"
     CDS             <1..156
                     /locus_tag="ACG8VH_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFP9111503.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG8VH_003026"
     CDS             169..1077
                     /locus_tag="ACG8VH_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFP9111504.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="ACG8VH_003027"
     CDS             1077..1697
                     /locus_tag="ACG8VH_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111505.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          613 a          251 c          395 g          472 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc a
//
LOCUS       DAZKCC010000184         1728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000184 DAZKCC010000000
VERSION     DAZKCC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.195"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8VH_003028"
     CDS             <1..156
                     /locus_tag="ACG8VH_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFP9111506.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG8VH_003029"
     CDS             169..1077
                     /locus_tag="ACG8VH_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFP9111507.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1694
                     /locus_tag="ACG8VH_003030"
     CDS             1077..1694
                     /locus_tag="ACG8VH_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111508.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          629 a          231 c          368 g          500 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
      841 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
      901 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
      961 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     1021 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     1081 ttaaagtaaa gagaatgatg gagactgatg aatctggtgt taaacgtcaa tttatgccaa
     1141 ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     1201 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     1261 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     1321 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     1381 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     1441 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaagggaaaa ttagaatctc
     1501 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     1561 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     1621 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     1681 tcggggaggt atagaaaatg acagatatcg taaaaataaa acaaagcg
//
LOCUS       DAZKCC010000185         1681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000185 DAZKCC010000000
VERSION     DAZKCC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.196"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..540
                     /locus_tag="ACG8VH_003031"
     CDS             118..540
                     /locus_tag="ACG8VH_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111509.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            550..753
                     /locus_tag="ACG8VH_003032"
     CDS             550..753
                     /locus_tag="ACG8VH_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111510.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            964..1548
                     /locus_tag="ACG8VH_003033"
     CDS             964..1548
                     /locus_tag="ACG8VH_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111511.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          595 a          232 c          303 g          551 t
ORIGIN      
        1 ttaaacgatt atgacattaa aaatatattt tatgaatttt ctagtatata acttgattta
       61 tctgttaaga tttattaaga aaacgttttc ttatttttga tttcgaggtg atagacaatg
      121 aaaaataagt ttattttatc tttaggtatt ttaactattg gaattagtgt aactggaact
      181 acaattccta catttgctga tagtacaaat gaaccaacta ctgtagtaga taataaccaa
      241 gaattaactc ctttagatag gaacggaata gccattggta acccaactaa cgacttaact
      301 gttactgaaa attatttaat agtttctcca ttacttgctc gagcagcttc taaaacgata
      361 cgagtgacag gaacgatgat ttttgataaa gttggttggc cttctagacc tcctcaatca
      421 cggaattgga aagaaaaaag aaatggttat tggtactatg gaaaattgta tttacaaagt
      481 tataaatcag ctggaaacaa ttggattgcg acttattcag gaaccttaac tagaaagtag
      541 ggatttttta tgccagaatt tgtgaacaca ccaaattttt atcaagatag cgatattcta
      601 agtaaaatta atttaccgaa agaagaatca gctttacaag ttcagcgaac tttacaggag
      661 acacaaaaaa ttcaaaaatc aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa
      721 agagaccaaa aaacatatca aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg
      781 acattctttt tttctaattg aaagtgttca taaaatcacc catttctgga cactttcttt
      841 ttttgcttta tttttcaaaa aaacaattca tttcatgttc ataaacccat aacaatatct
      901 atgttcagtt aaactatatg gataaagtta tgttacaata aaattaatga aggagtgatt
      961 tttttgaaaa taggatacgc ccgggtatct actggtttac aaaatttgga tttacaagaa
     1021 gacagcctaa ataaatttgg ttgtgaaaaa atttttactg atcatatgag tggggcaaaa
     1081 agtaatcgac caggattaga aatggccatt gattttgttc gctctggcga tacacttgtc
     1141 gtttggcgct tagatcgttt aggtagaaat atggaagatt tgatttcaat cgtcaatcga
     1201 ctgaatgagc gtggtgtgag ttttcatagt attcaagaaa atattacgat ggacaaatca
     1261 agttctacag gacagctcat gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat
     1321 ttgatcctgg aacgatccgc agcaggacga gcagctgctc gtgcaagagg acggtttggt
     1381 ggtcgtcctg aaaaattgtc tgaacaagat ttggaattac tgaagacttt agtggatagc
     1441 ggtacaccta ttaagactat tgcggaacgg tggaacgtct caagaaccac catttatcgt
     1501 tatttggcaa aaatggagga gaaaaatgga aagacaaatt ctcattagtt tttgtcattt
     1561 aatgctgaaa atttttgcca tttatctaga aaagtgacaa tagtatgaga attttttgta
     1621 agtgtctact gataacttat tctcaaatgt tgtaaggatt ttattcattt gttaatatta
     1681 a
//
LOCUS       DAZKCC010000186         1676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000186 DAZKCC010000000
VERSION     DAZKCC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.197"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8VH_003034"
     CDS             <1..189
                     /locus_tag="ACG8VH_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9111512.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1480)
                     /locus_tag="ACG8VH_003035"
     CDS             complement(1055..1480)
                     /locus_tag="ACG8VH_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFP9111513.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
BASE COUNT          526 a          333 c          198 g          619 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaattt
//
LOCUS       DAZKCC010000187         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000187 DAZKCC010000000
VERSION     DAZKCC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.198"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8VH_003036"
     CDS             131..505
                     /locus_tag="ACG8VH_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9111514.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8VH_003037"
     CDS             complement(619..1248)
                     /locus_tag="ACG8VH_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9111515.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8VH_003038"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8VH_003038"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKCC010000188         1556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000188 DAZKCC010000000
VERSION     DAZKCC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.199"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..1506
                     /locus_tag="ACG8VH_003039"
     CDS             40..1506
                     /locus_tag="ACG8VH_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP9111516.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          429 a          290 c          327 g          510 t
ORIGIN      
        1 taatattttc tgaaaattta aagaattggg gtatagatta tggaaaaaca gcaagacaag
       61 ggcttcttcg ggcagccgaa gggattgtca actttgttct tcactgagat gtgggaacga
      121 tttagttatt acggtatgcg tgcattactt atctattata tgtacgatac agttgccaac
      181 gggggacttg gattaccacg agcaacagca ttagctatca tgtcgatcta cggatctttg
      241 atctacatgt ctagtatcgt cggcggatgg gtatctgacc gagtgttagg agcaaaacgc
      301 actgtattct tcggtggagt tttcattatg cttggccaca tcgtcttggc tttaccattt
      361 ggtgtaagta cattgtttgt ttcgatggca ttgattatta tcggaaccgc ctttttgaaa
      421 ccaaatgttt ctggcatggt cggacatctt tattcaaaga ctgacttgcg aagagatgca
      481 ggattttcga ttttttacat gggtgtcaac ttaggggcat tattagcacc actaatcgtt
      541 ggtacaatcg gtcaagaata taactatcat ttagggttct ctttagctgc tattgggatg
      601 ttctttggat tattacaata catcatccaa ggacgcaaga cattagacgg agtgggtaca
      661 acaccgccga atcctttgac aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc
      721 gcacttgttg ttatcgctgc agtattcggc ggggcacaac taactggtca tctgacaatt
      781 gacttttttg tcaatacaat cagtgtattg gggattatct taccgttgta ttactttatc
      841 aaaatgctga cttcaaaaga tgtaactgct gaagaaaaac caagagtatg ggcttatatc
      901 ccattattcc ttgcagcaat cattttctgg tcattacaag aacaaggttc atctatctta
      961 gctttatttg ctagtgaacg tacacaatct acgctatttg gtgtaacgat ccaagcaagc
     1021 tggtatcaat cattgaatcc aattttcatc tttattctga caccagtatt cgtaacgatg
     1081 tggacgaaac ttggtgaacg tcaaccatct acagtagtga aattctctct tggactttta
     1141 tttgcaggat tatcgtactt gctattgatg ttgccgggta tgctttatgg tacaagtgga
     1201 aaagtaagtc cattgtggtt gttgggaagt ttcttcatcg tggttattgc tgaattgtgt
     1261 ctttcaccag ttggtttgtc tgtgacaaca aaattagcac cacgagcatt tgaatcacaa
     1321 acaatcgcta tttggttctt agctgatgct tcttcacaag caatcaatgc acaaattgca
     1381 cgcttctaca caccgggaac agaatctgct tacttcggta ttatcggtgc agtagcaatc
     1441 gtcggtggta tcttgctatt tactgtgaaa gaaccaatca aaaaactaat gggctctatt
     1501 cattaatcaa tgaacataaa aacaggaatt gcttatttgc agcagttcct gttttt
//
LOCUS       DAZKCC010000189         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000189 DAZKCC010000000
VERSION     DAZKCC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.200"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8VH_003040"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8VH_003040"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKCC010000190         1423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000190 DAZKCC010000000
VERSION     DAZKCC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.201"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1111
                     /locus_tag="ACG8VH_003041"
     CDS             <1..1111
                     /locus_tag="ACG8VH_003041"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9111517.1"
                     /translation="EVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDIPG
                     NNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDV
                     ADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDM
                     VVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSV
                     DFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESI
                     DYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQ
                     ITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            1223..>1423
                     /locus_tag="ACG8VH_003042"
     CDS             1223..>1423
                     /locus_tag="ACG8VH_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9111518.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESST"
BASE COUNT          563 a          182 c          235 g          443 t
ORIGIN      
        1 agaagttgac aaaattgata ttcaaaataa aaagattagt tgtgtaaaaa aagatggtac
       61 aagattctct caagaatatg acaaagttat attagctaca ggttcaaagc aaattatgct
      121 agatataccg gggaataatt tagctaatat tttttcgata aagaattttc caaatgcaaa
      181 agagattttt aaagctttgg atgatagtac tattaaaaaa gtaggaatta ttggagcagg
      241 atatattgga gttgaaattg ctgaagccat aagaaaaaga gggaaagaag tatatctttt
      301 cgatgttgca gatagagtgt tgtcgactta ttacgaccgt agtttttcag ataaagttga
      361 agaaatacta tcaaaaaatg gaatacatct ttgtctatca gaaagtccta aaaaatattt
      421 aggaagaaaa aagattgaaa aaatagtcac tacaagtggt acccaatact ctatggatat
      481 ggtcgtacaa gctataggat ttctatctaa tagtccaatt ggggaaaagg aactcttgag
      541 agatgttagt ggagcatatc taactaatga ataccagcaa acaaatataa aagatatata
      601 tgctattggg gattgtgcta caactttttt tacgtccatt aatagaagta gtgttgattt
      661 ttctatttct aatgctctaa gaacagctta tattgcaact caacatatta acaatcaaga
      721 ttttacacct tctggttccc aatttacaaa tggcttttct atatttaatt acaattttta
      781 cgcagcaggt ttgaatttaa aaacagcacg attattaagt gagtctatag attatataga
      841 aatagaagat ttcattagac ctgctttttt gagaaaaaat agtcgtgtaa aattaagaat
      901 tagctttgaa aaaaatacaa agagaatagt aggtgtacaa ctttgtagtc aagaagattt
      961 atctggacta ttatcaatgt tttctcttgc tatagaagaa caaattacat tagacaaatt
     1021 gaaaatgtta gattatttat ttcatcctag ttttagccaa ccctataatt ttattttaaa
     1081 ggcagtaaca ggattaaaca ttgaaacata atcgttacta aatgaaatca agaaatatac
     1141 tgtattactc aattcattga aaatatttat cataattttt aatagaaaac actattaaag
     1201 agaaaaaaga gaggtaaatc aaatgaaaaa atctttaaaa cttgcatcag cagttatatt
     1261 aatgtcccaa ctccttatag caacaccttt ccatgtgttg gcaagcgaaa attcaacata
     1321 caaaattgca aaagaaagaa ccgagacgac tgaaatgagt aattccgatg taacagcaga
     1381 gagtagtgaa cttttgacaa cttcatcaag tgaaagtagt acg
//
LOCUS       DAZKCC010000191         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000191 DAZKCC010000000
VERSION     DAZKCC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.202"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8VH_003043"
     CDS             complement(150..1337)
                     /locus_tag="ACG8VH_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP9111519.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKCC010000192         1374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000192 DAZKCC010000000
VERSION     DAZKCC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.203"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..1340
                     /locus_tag="ACG8VH_003044"
     CDS             39..1340
                     /locus_tag="ACG8VH_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9111520.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          275 c          253 g          398 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DAZKCC010000193         1371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000193 DAZKCC010000000
VERSION     DAZKCC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.204"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8VH_003045"
     CDS             85..624
                     /locus_tag="ACG8VH_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111521.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          518 a          174 c          208 g          471 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt t
//
LOCUS       DAZKCC010000194         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000194 DAZKCC010000000
VERSION     DAZKCC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.205"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG8VH_003046"
     CDS             82..>1364
                     /locus_tag="ACG8VH_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP9111522.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZKCC010000195         1354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000195 DAZKCC010000000
VERSION     DAZKCC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.206"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8VH_003047"
     CDS             <1..379
                     /locus_tag="ACG8VH_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9111523.1"
                     /translation="SCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEGGVQD
                     IISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDEIRSF
                     VPREIRCVARNLIYLQNNNILNS"
     gene            393..749
                     /locus_tag="ACG8VH_003048"
     CDS             393..749
                     /locus_tag="ACG8VH_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9111524.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          495 a          203 c          253 g          403 t
ORIGIN      
        1 ttcctgtaga aaagcaagag catggttgca agcgaatcaa cttgaatttg aagagaagaa
       61 tatttttgcc gaaccgttaa cagaaagtga gatcaaaaag atcttggcag cgactgaagg
      121 aggagttcaa gatattattt ccactcggtc gaaaatttac gaaaagctgg gcatagattt
      181 taacgaatta actttaaagc aaatgatcgt cttattcaaa gaatatcctt cattattgag
      241 acgacctatt ttgattgatg aaaaaagaat actaatcgga tttaatgaag atgagatccg
      301 gtcatttgtc cctcgtgaaa ttcgatgtgt tgccagaaat ctgatctatt tacaaaacaa
      361 taacatctta aattcatagg gagggagcct tcgtggaagt gaaagtctat ggatcagcgc
      421 actgtgtaac aagtctgaag gcgaaacaat ggttagaaca ccatcaattg ccttatcaat
      481 ttgttgactt agaagaacgc gatctaagta cagaagaagc agaagaatta tgcagttttg
      541 aagaagtgca agcagaacag ctatttgcga cttggtctga ggcttttcac aacattgcga
      601 ttgacctttc ctgtgtagga aaaaaacaac tgattttttt atgcacacaa cataccaatc
      661 tcctcagacg tcctttgatt attatcgatg aagttctatt tattggatac aatgagcagt
      721 tgatggaaca gcttatctta aataaataga ctttaactgt tttaatcaag tcactgagca
      781 tttcatacag ttgtaacagt cgaacactta gcatcaagcg tgttatgtgt tctttttttg
      841 actttatttt tctactatag agacatgctc aatttatgtg gtcaaaattc aaatctaggt
      901 ggggatatag agttaaaaat atattgacat gtgtaaattt acatgatata ttcatttctg
      961 ttgttttaag tagcaaagca ataaaatagt attttaaaaa acatataaaa tagttgttga
     1021 catgttagaa atgacatgat aagataactg agttgttaaa aaagaaccac ttgtgaagct
     1081 tgattctatc gaaaaaaact tcaaaaaagt tcttgacaaa taacaaatga taagttatga
     1141 taaatgagtt gctgtaagat ttcgaaaaac gaaatcaaaa aaacttttaa aaaagagttg
     1201 acaattactt gtctctctga tatgatataa aagttgctac ggcagccaaa aacgaatcgg
     1261 ggaacactcc tgatgaagta gacctttgaa aactgaacaa agtaagacaa accaaatgtg
     1321 tagggcgtct tgattcaatt caagacaaca aaca
//
LOCUS       DAZKCC010000196         1353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000196 DAZKCC010000000
VERSION     DAZKCC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.207"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            552..1085
                     /locus_tag="ACG8VH_003049"
     CDS             552..1085
                     /locus_tag="ACG8VH_003049"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9111525.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          430 a          249 c          254 g          420 t
ORIGIN      
        1 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg
       61 ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
      121 atttttcgtt tggttggact tattaccgca gatgcaagct tttgaacacc gtttattgag
      181 aagttgtgaa atcagcgtcc tgacctgagt attagagaaa caaatggcaa aatagttcct
      241 catattttgc tattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg
      301 agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa atttttttga aaatggttcc
      361 tcacaacttt gcataaattt gacttaatac cgagaagtca tgatccccat ctaactccaa
      421 atcacaagga atgagttcaa aaactagctt ttctcaacaa atattacata aaaaattcta
      481 tgttttttgt taaatttatt tgcttttttc aaaagtatgt tacagtaatt tcccaaggag
      541 gttttacaga aatgaatttt gttgccatgg attttgaaac tgccaatcat cagccttata
      601 gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat gaattctata
      661 cattgataca gcctgagact ccttttttct ggcgaaatgt ccaaattcac ggaatccatc
      721 aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc caaccatatt
      781 ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt tgatacaaag gtcttagcgg
      841 gatgcttaga ttattatcat cttgaacagc ctaattatct ttctttatgt acagtgaaaa
      901 gtagccgtcg cctgtttcct gaatttccaa atcatcgatt gaacactgtg tgcgaaaatt
      961 tgaacatcca attagatcat caccatgatg cattggaaga tagtcgtgca tgtgcggaaa
     1021 tcctgcttta tcaagaaaaa aaattcggga cagatccatt gaaaaaactt gtgactataa
     1081 agtaaaagaa tacttatgaa acacacaaag aggtcgtgag cctgccgttt cactccgagt
     1141 cacaaggagc aatttcaaaa aatagcttct catgtttatt aaatattgtg actggtgacc
     1201 gaggagctgg caggagaaca ccgtttattg gagaggttgt gaaaaagctg ttctgcatca
     1261 agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt tggacttatt
     1321 accgcagatg caagcttttg gacaccgttt acc
//
LOCUS       DAZKCC010000197         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000197 DAZKCC010000000
VERSION     DAZKCC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.208"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8VH_003050"
     CDS             <1..1244
                     /locus_tag="ACG8VH_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9111526.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKCC010000198         1282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000198 DAZKCC010000000
VERSION     DAZKCC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.209"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..779
                     /locus_tag="ACG8VH_003051"
     CDS             <1..779
                     /locus_tag="ACG8VH_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFP9111527.1"
                     /translation="DGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNY
                     RAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVS
                     KEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANL
                     HVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMR
                     NFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            969..1042
                     /locus_tag="ACG8VH_003052"
     tRNA            969..1042
                     /locus_tag="ACG8VH_003052"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1003..1005,aa:Ile,seq:cat)
BASE COUNT          414 a          213 c          263 g          392 t
ORIGIN      
        1 gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg ggagctggta
       61 acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat aaagcattgt
      121 ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa aaatatggaa
      181 ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt tctcatttgt
      241 gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta tcgcaaatgg
      301 gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat gaaaatccaa
      361 caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct aatcctacgc
      421 tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa aacgcaaact
      481 tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc attatggact
      541 acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga ccagatgtaa
      601 accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat atgcgtaatt
      661 ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag ggaaacacta
      721 aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct aaacgataaa
      781 aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa attattcgat
      841 aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca ctccaagcta
      901 tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc tttatgtggc
      961 aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg tccggtcgta
     1021 ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg ttagaatttt
     1081 tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg agtggctttt
     1141 ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact tctgtatatt
     1201 tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg tgagataata
     1261 ataaagaaga gtttaaagcg ca
//
LOCUS       DAZKCC010000199         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000199 DAZKCC010000000
VERSION     DAZKCC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.210"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG8VH_003053"
     CDS             complement(31..1203)
                     /locus_tag="ACG8VH_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFP9111528.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DAZKCC010000200         1267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000200 DAZKCC010000000
VERSION     DAZKCC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.211"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1244
                     /locus_tag="ACG8VH_003054"
     CDS             66..1244
                     /locus_tag="ACG8VH_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9111529.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          400 a          275 c          266 g          326 t
ORIGIN      
        1 aaaaaggaag tcccttcagt agaatagagt taccacaaca cattcacaga aaagaggact
       61 tccatatgaa cgattttact acagaaattc taaagactct agcgaataaa ggcgatttga
      121 atgaattatt ccgtgtccat ttggaaaaag ctgtcaatac gcttctcaaa acggagttaa
      181 cggctttcct cgattacgaa aagtacgatc gcattggttt taacacgggt aattctcgta
      241 acggctccta tgaccgtacg gtcaagaccg agtacgggga acttcatctc cagattccgc
      301 gcgaccgcaa cggcgagttc aagcaacaga ctgttcctgc ttatagacgg acgaatgata
      361 ccttagagga gaccgtcatt cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag
      421 acttgattga gaaaatgtat gggcatcact acacgcccca aaccatgtcc aatatgacta
      481 aatcatttac ggaagaagtc acttcattta aggaacgtga gcttcatgac cgttatgccg
      541 ctatttatat ggacgcaacc tatatccctt taaagcggaa aaccgtcgcc aaggaagcca
      601 ttcatatcgc agttggcatt cgcccggacg gttcaaagga agtgttgagc tatgcgattg
      661 caccaactga atctatcacg atttgggaag agattttaat agacctccag gaacgtggcg
      721 tgaaaaacgt cctccttttc atcacggatg gcttaaaggg gatggcaggg gccgtgcagc
      781 ggttctaccc caaagcccgt tttcaacatt gctgtgtgca cgtttcccgt aatatcagtc
      841 acaaagtgcg tgtcgatgat cgtaaggaag tctgtgatga ttttaaaatg gtgtatcaag
      901 cgtcatctaa aaaggcggca ttggaagcac gtggtgcttt tgcggaaaaa tggaaaacca
      961 gttatccaaa aatggtggaa tcgatccttt cgaacgatta cctgctcact ttctatgatt
     1021 ttccattagc catacgcaag actatttact ctacgaactt gattgaatcc tttaataagc
     1081 aaatcaagaa atacagccac agaaaagagc agtttcaaaa tgaagaatca ctagagcgtt
     1141 tcctagtatc catctttgat acatacaatc aaaaattctt aaatagaagc cataaaggct
     1201 tccaacaagt gacggataca ttagcttcaa tgtttactga gtaacccatt attttgcaga
     1261 aggacga
//
LOCUS       DAZKCC010000201         1262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000201 DAZKCC010000000
VERSION     DAZKCC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.212"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG8VH_003055"
     CDS             283..522
                     /locus_tag="ACG8VH_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111530.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACG8VH_003056"
     CDS             complement(884..1144)
                     /locus_tag="ACG8VH_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111531.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          424 a          227 c          171 g          440 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aa
//
LOCUS       DAZKCC010000202         1259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000202 DAZKCC010000000
VERSION     DAZKCC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.213"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..1087
                     /locus_tag="ACG8VH_003057"
     CDS             230..1087
                     /locus_tag="ACG8VH_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9111532.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          465 a          184 c          253 g          357 t
ORIGIN      
        1 gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat aacgtcatta
       61 ctttgaggaa agctataaat caatatattt cagcttttcc ttatttatgt actacctttt
      121 acttattaat tataaggtag tacatatata tctttttttg gtagtaacta aaaataaata
      181 tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg tggtagcgaa
      241 gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg cgcaaatgtt
      301 agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact tggatatgca
      361 aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg aagaaggagg
      421 cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga taaacaatct
      481 tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc cagcagacat
      541 gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat tatcccaatt
      601 caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca tctatttaga
      661 tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg actgggcaat
      721 cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc ttgagtatat
      781 ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta ttcctttcat
      841 gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag agatttatgg
      901 ggaacatttg attaaaaatg ttgtactcaa aaatgcgcgc ttaaaacgat acgacggatc
      961 aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc gcaaaagcca
     1021 cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt atgcggaggt
     1081 ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag gactcgccaa
     1141 aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat ctgaaaataa
     1201 gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg aaagacaga
//
LOCUS       DAZKCC010000203         1246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000203 DAZKCC010000000
VERSION     DAZKCC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.214"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8VH_003058"
     CDS             200..742
                     /locus_tag="ACG8VH_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111533.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG8VH_003059"
     CDS             898..>1246
                     /locus_tag="ACG8VH_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP9111534.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZKCC010000204         1223 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000204 DAZKCC010000000
VERSION     DAZKCC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.215"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..484
                     /locus_tag="ACG8VH_003060"
     CDS             <1..484
                     /locus_tag="ACG8VH_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9111535.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1223)
                     /locus_tag="ACG8VH_003061"
                     /pseudo
     CDS             complement(691..1223)
                     /locus_tag="ACG8VH_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          388 a          232 c          236 g          367 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atc
//
LOCUS       DAZKCC010000205         1097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000205 DAZKCC010000000
VERSION     DAZKCC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.217"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..318)
                     /locus_tag="ACG8VH_003062"
     CDS             complement(<1..318)
                     /locus_tag="ACG8VH_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111536.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFS"
     gene            complement(320..>1097)
                     /locus_tag="ACG8VH_003063"
     CDS             complement(320..>1097)
                     /locus_tag="ACG8VH_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9111537.1"
                     /translation="IFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKI
                     KWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQH
                     SSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELK
                     RAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFIL
                     SIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          420 a          185 c          134 g          358 t
ORIGIN      
        1 ggagaaaagt atttttgtga aataattttc cccctgtata aaaatttcat cataaacgga
       61 taaatcatcc gagaaaaatt gattatggtt agttaacaag ttaattttac gtttaaagga
      121 agaaccgatt ttccggtgat tcacaaaaac aacatcatta aatagcccta attcttttat
      181 acgctcacta tatcttctag cgttttcaaa ttcagaataa ataaataaat ctccttttga
      241 ttctcggtaa aaattttgat aaacatttaa tatattaatc aaatgtaaag gagtccaagc
      301 aatgaaagct gtcttcatat taattctcct tttttaaata caagtaagaa atgcttccca
      361 aataatacca gaaaacaatt gaaatgacat cgcgcttatt gaaaagtaca tgagcctcca
      421 ccatttcatc tataaaaata gatagtataa aaattaaaac aataccaaga aacgcataat
      481 tcactttact tctaaaaaat gttttgagta aactaaagat atttaacaca tagaaagcat
      541 agatacatat aaaagcaatt ataccacttg atacaagtac ttgaagaaat ccattatgca
      601 tgtttccatg tgctcttttt agttcattca catctgtagt atcaagatct acttttttca
      661 aaaattttgc atcgctattc ccatagaaat cggtctctcc ataaccaaat aacaaagttg
      721 gtcttataac tttaagcgca gctttccaaa tcgtaaaacg accagctgaa atttcggagt
      781 tatcactaga atgctgtaat attacttctt tttctggaag tggagtttta tctgattcat
      841 ttgactctat aacttctact tttctatcaa tcgatacagg aattgttgct aaaaaatcct
      901 tagcataatt agtaccaaaa gaaaaatata ccaatagaac taataatatg cttaccatcc
      961 atttgatttt tacaggtagt acttctctaa tgctctttct aaattttgaa ttggcaaaca
     1021 acaaaagaaa taatactaaa aaagaacaaa ttactacctg ggcacctcta gacgaagaaa
     1081 gtgtaatata aaatatt
//
LOCUS       DAZKCC010000206         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000206 DAZKCC010000000
VERSION     DAZKCC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.218"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8VH_003064"
     rRNA            <1..>1073
                     /locus_tag="ACG8VH_003064"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKCC010000207         1067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000207 DAZKCC010000000
VERSION     DAZKCC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.219"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8VH_003065"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8VH_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9111538.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DAZKCC010000208         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000208 DAZKCC010000000
VERSION     DAZKCC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.220"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG8VH_003066"
     CDS             <1..963
                     /locus_tag="ACG8VH_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9111539.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          366 a          195 c          212 g          289 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DAZKCC010000209         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000209 DAZKCC010000000
VERSION     DAZKCC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.221"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACG8VH_003067"
     CDS             complement(37..>1062)
                     /locus_tag="ACG8VH_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFP9111540.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZKCC010000210         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000210 DAZKCC010000000
VERSION     DAZKCC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.222"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG8VH_003068"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG8VH_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKCC010000211         1001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000211 DAZKCC010000000
VERSION     DAZKCC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.223"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8VH_003069"
     CDS             <1..459
                     /locus_tag="ACG8VH_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111541.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8VH_003070"
     CDS             473..904
                     /locus_tag="ACG8VH_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111542.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..>1001
                     /locus_tag="ACG8VH_003071"
     CDS             906..>1001
                     /locus_tag="ACG8VH_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9111543.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT          373 a          146 c          212 g          270 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga a
//
LOCUS       DAZKCC010000212          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000212 DAZKCC010000000
VERSION     DAZKCC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.224"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8VH_003072"
     CDS             <1..878
                     /locus_tag="ACG8VH_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9111544.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          301 a          212 c          192 g          256 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      961 a
//
LOCUS       DAZKCC010000213          933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000213 DAZKCC010000000
VERSION     DAZKCC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.225"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8VH_003073"
     CDS             <1..355
                     /locus_tag="ACG8VH_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111545.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..933
                     /locus_tag="ACG8VH_003074"
                     /pseudo
     CDS             615..933
                     /locus_tag="ACG8VH_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          357 a          110 c          196 g          270 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt cca
//
LOCUS       DAZKCC010000214          902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000214 DAZKCC010000000
VERSION     DAZKCC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.226"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..282)
                     /locus_tag="ACG8VH_003075"
     CDS             complement(<1..282)
                     /locus_tag="ACG8VH_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111546.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
     gene            complement(384..737)
                     /gene="tnpB"
                     /locus_tag="ACG8VH_003076"
     CDS             complement(384..737)
                     /gene="tnpB"
                     /locus_tag="ACG8VH_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9111547.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          258 a          185 c          158 g          301 t
ORIGIN      
        1 tttcgttcct acaactcgtt gatgtccttt tactggaata atggtggcag ctgcttctgg
       61 aacacttaca gaaatttctt catcactaaa aagattcaat tgcccattcg tttcctcaaa
      121 gagagtctct ttagagcgtc caaataattt gttggttaaa taattgacct tttcattcaa
      181 tagttggatt tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat tggttttatg
      241 ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc accaccttga
      301 atcgaactga ttttgttaca gaataccaca agaaacgcac aagaaatagg ggttcaacac
      361 attttttgtg ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga
      421 ggttgatcga tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga
      481 acttcgtctt tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga
      541 agaaaaccat caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat
      601 aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga
      661 ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga
      721 agtaaatcat aaagcattgg acaacagttc tttcaaaata gcttgaagaa tataattgtt
      781 aataccgtta tagatcaaaa cttcagaacc attctctctt ttgatgcgca tcgctaactt
      841 ggcagccgcg ggtttacctt tttctggtgc actcttttcg tttcctaatt ggacaggaac
      901 aa
//
LOCUS       DAZKCC010000215          845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000215 DAZKCC010000000
VERSION     DAZKCC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.227"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..104)
                     /locus_tag="ACG8VH_003077"
     tRNA            complement(32..104)
                     /locus_tag="ACG8VH_003077"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:His,seq:gtg)
     gene            complement(107..180)
                     /locus_tag="ACG8VH_003078"
     tRNA            complement(107..180)
                     /locus_tag="ACG8VH_003078"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Trp,seq:cca)
     gene            complement(187..267)
                     /locus_tag="ACG8VH_003079"
     tRNA            complement(187..267)
                     /locus_tag="ACG8VH_003079"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Tyr,seq:gta)
     gene            complement(269..344)
                     /locus_tag="ACG8VH_003080"
     tRNA            complement(269..344)
                     /locus_tag="ACG8VH_003080"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Phe,seq:gaa)
     gene            complement(380..452)
                     /locus_tag="ACG8VH_003081"
     tRNA            complement(380..452)
                     /locus_tag="ACG8VH_003081"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(417..419),aa:Asp,seq:gtc)
     gene            complement(462..534)
                     /locus_tag="ACG8VH_003082"
     tRNA            complement(462..534)
                     /locus_tag="ACG8VH_003082"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Val,seq:tac)
     gene            complement(550..621)
                     /locus_tag="ACG8VH_003083"
     tRNA            complement(550..621)
                     /locus_tag="ACG8VH_003083"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(586..588),aa:Glu,seq:ttc)
     gene            complement(636..725)
                     /locus_tag="ACG8VH_003084"
     tRNA            complement(636..725)
                     /locus_tag="ACG8VH_003084"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(689..691),aa:Ser,seq:gga)
     gene            complement(765..838)
                     /locus_tag="ACG8VH_003085"
     tRNA            complement(765..838)
                     /locus_tag="ACG8VH_003085"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(802..804),aa:Asn,seq:gtt)
BASE COUNT          237 a          232 c          192 g          184 t
ORIGIN      
        1 taccgcttgg ctatacccca agaataaaaa gggcgaatga tgggaatcga acccacgaat
       61 gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatggc aggggcagca
      121 ggaattgaac ccacactaac ggttttggag accgctgttc tacctttaaa ctatgcccct
      181 ataaaatgga ggagagtgga ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt
      241 cgcgtttagc cacttcgcta ctcctccatg gtggcgcggg acagaatcga actgccgaca
      301 cacggagctt caatccgttg ctctaccaac tgagctaccg cgccatattt taaaattatt
      361 aattgaataa caagaacgac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca
      421 ggcaggcatg ttaaccccta caccacggaa ccagttcatt atggaggtta acgggatcga
      481 accgctgacc ctctgcttgt aaggcagatg ctctcccagc tgagctaaac ctccaacatt
      541 caattaaaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct
      601 taaccgcttg accaacgggc caggtaaatt tgctacggag agtaagggat tcgaaccctt
      661 gagacagttt ggcaccatct acatgatttc caatcatgct ccttcggcct ctcggacaac
      721 tctccagagt gaaagaagct caatactctt tctcaaaatg agaactccgg cagtaggact
      781 cgaacctacg acatcatgat taacagtcat gcgctactac caactgagct atgccggaat
      841 aacat
//
LOCUS       DAZKCC010000216          822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000216 DAZKCC010000000
VERSION     DAZKCC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.228"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG8VH_003086"
     CDS             complement(<1..153)
                     /locus_tag="ACG8VH_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111548.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            231..809
                     /locus_tag="ACG8VH_003087"
                     /pseudo
     CDS             231..809
                     /locus_tag="ACG8VH_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          259 a          151 c          158 g          254 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DAZKCC010000217          812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000217 DAZKCC010000000
VERSION     DAZKCC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.229"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..673
                     /locus_tag="ACG8VH_003088"
     CDS             <1..673
                     /locus_tag="ACG8VH_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9111549.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          236 t
ORIGIN      
        1 tagcggtaaa tactatattt ctttgttgtg taaagaagaa attatcgaat tacctaaaac
       61 taattccaca attggtattg acttaggtat tatggacttt gcgattcttt ctgatggaca
      121 aaaaattgat aataataaat tcacgtccaa aatggaaaag aaactaaagc gtgaacaacg
      181 caaattgtct aggcgtgctt tattggctaa aaataaaggt accaatctat ttgaagctaa
      241 aaattatcaa aaacaaaaac gcaaagtagc tagattgcat gaaaaagtaa tgaatcaacg
      301 tactgatttt ctgaataagt tgagtacaga aatgatcaaa aatcacgata ttatctgtat
      361 tgaagactta aacacaaaag gtatgttgcg aaatcataaa ttagctaaaa gtatttctga
      421 tgtttcttgg tcaaagtttg tgactaaatt acaatacaag gctgactggt atggacgaaa
      481 aatcatcaaa gtagataaat ggtttccgtc tagtcagatt tgttcagagt gcggacataa
      541 agatggcaag aaatctcttg aaattcgaga atggacttgt cctgtttgtc atactcatca
      601 tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa ggtctaagac tacattctat
      661 gggattggct tagatccaat catcaagaac cgtaggaact acggggatag cttggtaaat
      721 aagagacacc gctgttagta aagaaatacg ctatcaagta tgctctattc ccaagaagct
      781 cccacttcaa gcgttaagaa ccgtcaggtt ta
//
LOCUS       DAZKCC010000218          749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000218 DAZKCC010000000
VERSION     DAZKCC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.230"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..>749
                     /locus_tag="ACG8VH_003089"
     CDS             256..>749
                     /locus_tag="ACG8VH_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111550.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATG"
BASE COUNT          263 a          115 c          138 g          233 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagg
//
LOCUS       DAZKCC010000219          731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000219 DAZKCC010000000
VERSION     DAZKCC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.232"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACG8VH_003090"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACG8VH_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          268 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa a
//
LOCUS       DAZKCC010000220          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000220 DAZKCC010000000
VERSION     DAZKCC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.233"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8VH_003091"
     CDS             <1..>725
                     /locus_tag="ACG8VH_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFP9111551.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKCC010000221          716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000221 DAZKCC010000000
VERSION     DAZKCC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.234"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..422
                     /locus_tag="ACG8VH_003092"
     CDS             93..422
                     /locus_tag="ACG8VH_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9111552.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="ACG8VH_003093"
     CDS             412..699
                     /locus_tag="ACG8VH_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9111553.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          272 a           80 c          136 g          228 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttct
//
LOCUS       DAZKCC010000222          713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000222 DAZKCC010000000
VERSION     DAZKCC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.235"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..460
                     /locus_tag="ACG8VH_003094"
     CDS             197..460
                     /locus_tag="ACG8VH_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9111554.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            460..>713
                     /locus_tag="ACG8VH_003095"
     CDS             460..>713
                     /locus_tag="ACG8VH_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9111555.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          262 a           94 c          146 g          211 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      241 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      301 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      361 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      421 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      481 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      541 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      601 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      661 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gac
//
LOCUS       DAZKCC010000223          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000223 DAZKCC010000000
VERSION     DAZKCC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.236"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACG8VH_003096"
     CDS             <1..600
                     /locus_tag="ACG8VH_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9111556.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DAZKCC010000224          707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000224 DAZKCC010000000
VERSION     DAZKCC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.237"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..200)
                     /locus_tag="ACG8VH_003097"
     CDS             complement(<1..200)
                     /locus_tag="ACG8VH_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9111557.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGV"
BASE COUNT          270 a          110 c          108 g          219 t
ORIGIN      
        1 tatacaccct tcttcattct agttttcgca gcaggaattt tgaaatagtt cttattagaa
       61 tagtagagat tgattaataa gctatcttcg attgctaaaa agaaaacttc tgaatcccac
      121 accttataaa aatctttgac aaatttttct gatggatcgt atttaaacca aagcagatgt
      181 tcctctagct cgattgtcat gtttttcacc tcaacgaaag tatacgaact aatgtttgtt
      241 ttgtaaagga taaaaatagg gtagcaaccg gtacccaaac tagtacccat ttatcaaaaa
      301 cgacgaaatt tatgtaaata gaaaaataag aataaacatt gatgtatcaa catttatata
      361 tctttagaac aaatgaaaag ggtggttctc ataagaaaaa catgttcgca cttctaggca
      421 aaccaggatt tgaagacctt gctaaagaat tgaacgaacg cctgtaaaat aaggatttgt
      481 cgctagattt aaaaaatcag tgatagaaac atgtttgctt taaaatgaaa ctagtgatag
      541 ttttagtgat agtgaactta aaaaaagcac tcaatttgag tgctttttta ttgttattaa
      601 aaattaaaaa actaaaatac atacccgaat ttcatacccg aaatataaaa aatgtcatca
      661 cgtacatctt tttcgaaact accgaagtag ccaaagatgt actgatg
//
LOCUS       DAZKCC010000225          692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000225 DAZKCC010000000
VERSION     DAZKCC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.238"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..346
                     /locus_tag="ACG8VH_003098"
     CDS             134..346
                     /locus_tag="ACG8VH_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111558.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            508..>692
                     /locus_tag="ACG8VH_003099"
     CDS             508..>692
                     /locus_tag="ACG8VH_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9111559.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          246 a          103 c          121 g          222 t
ORIGIN      
        1 taaaaacact atatattccg aaaagtgagt atctccattc ataaaataat caacatattt
       61 aaaaaatcta attagctttt ttagtcaatc taaagttctt tattctatga tataatgtta
      121 ctgtagaaag tttgtggcgg tttggcaaat ccaattgaag gagcgtggtg cctatgggct
      181 tattagctta tcggacgtac tctgtagaaa ggaaatgcct gatttgttct cattggatca
      241 aatcattgct attatcggag cagcagcagt agttattact gccgtagcga aagctagcga
      301 ttcgatttta gcttggttaa agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt
      361 ttggcgaggt agacgagctc taaagcttta tttacatgcc atccgctttg aacggactac
      421 actgggcatg tgacagcatg ccctttcatt tatattatat ctcattcttt tatataatga
      481 aagtaaataa atcatggagg ataaaatatg gataaattta atagattaac attaataaat
      541 cagtttgaaa ttttaaaagc tttaaatcct aacgaagaaa aatactatgc tgaaaaaata
      601 gaaatactaa cagaaggtta cgaatatcac tatagtgaaa tatttgaaaa tatttctgat
      661 ccattgccag aagaagattc gcggtttgtt ct
//
LOCUS       DAZKCC010000226          640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000226 DAZKCC010000000
VERSION     DAZKCC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.239"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(158..460)
                     /locus_tag="ACG8VH_003100"
     CDS             complement(158..460)
                     /locus_tag="ACG8VH_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9111560.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          225 a          106 c           83 g          226 t
ORIGIN      
        1 gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc
       61 atcatattac acaatcttaa tactaactta aaaaatatcc cttttataag tatggtgata
      121 aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac ctttctggat
      181 caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg tctggcacat
      241 cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt gttgccggaa
      301 ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct tcgattgcta
      361 aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat
      421 caaatttaaa ccataattgt gtttttcctt ccatcaacat aatgttcacc tcaaaaagag
      481 tatacgaact aatgttcttt ttgtaaagta ggaatatcat tgataatatg gatggaacat
      541 atatattaaa aagtgttctt tttgaatgct attttgaatg ccattttcat tcatttaacg
      601 aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
//
LOCUS       DAZKCC010000227          625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000227 DAZKCC010000000
VERSION     DAZKCC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.240"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>625)
                     /locus_tag="ACG8VH_003101"
     CDS             complement(109..>625)
                     /locus_tag="ACG8VH_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFP9111561.1"
                     /translation="IVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKE
                     AFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYK
                     SVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDN
                     ERGSDTSGEAA"
BASE COUNT          183 a          144 c          115 g          183 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatg
//
LOCUS       DAZKCC010000228          621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000228 DAZKCC010000000
VERSION     DAZKCC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.241"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACG8VH_003102"
     CDS             complement(<1..448)
                     /locus_tag="ACG8VH_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111562.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          181 a          113 c           85 g          242 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg c
//
LOCUS       DAZKCC010000229          613 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000229 DAZKCC010000000
VERSION     DAZKCC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.242"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..379
                     /locus_tag="ACG8VH_003103"
     CDS             44..379
                     /locus_tag="ACG8VH_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111563.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          241 a           68 c          108 g          196 t
ORIGIN      
        1 aatttcctag gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa
       61 tgaatggaaa gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg
      121 cttagagaag gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc
      181 agaccaattt ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa
      241 tgatggaaaa tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac
      301 atgggaaatg agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat
      361 ttcacacacc gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat
      421 ccttttttca atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta
      481 tatcaaaata aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt
      541 atatttatta ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaagtaaat
      601 atacgctata aaa
//
LOCUS       DAZKCC010000230          591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000230 DAZKCC010000000
VERSION     DAZKCC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.243"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..564
                     /gene="spxA"
                     /locus_tag="ACG8VH_003104"
     CDS             166..564
                     /gene="spxA"
                     /locus_tag="ACG8VH_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP9111564.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          206 a           92 c          114 g          179 t
ORIGIN      
        1 ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt atgaaaaaaa
       61 ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta ttatgatctt
      121 ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt aacattatat
      181 acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga acatcagatt
      241 ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt aaaagcgatt
      301 ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa agtattccaa
      361 aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact tgtgcaaaac
      421 aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca agttggtttt
      481 aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga attacgtcaa
      541 gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga c
//
LOCUS       DAZKCC010000231          588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000231 DAZKCC010000000
VERSION     DAZKCC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.244"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG8VH_003105"
     tRNA            complement(65..150)
                     /locus_tag="ACG8VH_003105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG8VH_003106"
     tRNA            complement(167..238)
                     /locus_tag="ACG8VH_003106"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG8VH_003107"
     tRNA            complement(250..322)
                     /locus_tag="ACG8VH_003107"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG8VH_003108"
     tRNA            complement(340..421)
                     /locus_tag="ACG8VH_003108"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG8VH_003109"
     tRNA            complement(429..501)
                     /locus_tag="ACG8VH_003109"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG8VH_003110"
     tRNA            complement(505..577)
                     /locus_tag="ACG8VH_003110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZKCC010000232          539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000232 DAZKCC010000000
VERSION     DAZKCC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.245"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          189 a          102 c           57 g          191 t
ORIGIN      
        1 gaaacaattt tttctgcttg ttacctaaat taaatcacaa ctaaaggtaa cagtcaacaa
       61 agatttgtta ctaatctaat tttctatttt ttcgccttcc aaatcaagaa aaccgaaggt
      121 aacgtcttat cctagtgtga tacctcaaat gtccactttt gttacctttg ctgtccaaaa
      181 ctgttaccct tactgtccac ttttgttacc tttactgtcc aaaagtgtta cctttatacc
      241 cctttaatac ttactctccc aagagattaa gcctccccta acagatatat taacagatat
      301 ttaacagata tattaacaga tatttaataa aggaggatta ctcctctctt atttcaaaat
      361 cttaaaaata aaaaatctta aaaaaaacta acactatcta ggagtttatt ttgcttgtgt
      421 aactttgaat aattgaaaaa aaggaaaagg aggaagcttc agcttcccct tttatttata
      481 tttataatat atattttaat ctttaaaact ttagcaacct ccaaaaaggc tttatatca
//
LOCUS       DAZKCC010000233          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000233 DAZKCC010000000
VERSION     DAZKCC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.246"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG8VH_003111"
     CDS             <1..343
                     /locus_tag="ACG8VH_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111565.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DAZKCC010000234          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000234 DAZKCC010000000
VERSION     DAZKCC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.247"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..508
                     /locus_tag="ACG8VH_003112"
     CDS             <1..508
                     /locus_tag="ACG8VH_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFP9111566.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          155 a           89 c          110 g          154 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaa
//
LOCUS       DAZKCC010000235          494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000235 DAZKCC010000000
VERSION     DAZKCC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.248"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..164
                     /locus_tag="ACG8VH_003113"
     CDS             42..164
                     /locus_tag="ACG8VH_003113"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111567.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            137..268
                     /locus_tag="ACG8VH_003114"
     CDS             137..268
                     /locus_tag="ACG8VH_003114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111568.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          184 a           73 c           87 g          150 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atcgccgatt gtaaaattaa
      361 gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat
      421 aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag
      481 tcttatttca aaat
//
LOCUS       DAZKCC010000236          487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000236 DAZKCC010000000
VERSION     DAZKCC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.249"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACG8VH_003115"
     CDS             complement(<1..>487)
                     /locus_tag="ACG8VH_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111569.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAZKCC010000237          485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000237 DAZKCC010000000
VERSION     DAZKCC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.250"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          159 a           95 c           56 g          175 t
ORIGIN      
        1 atcactctac aataacggcg aaagcacaaa aaataaaaaa attattacgt tattaaaaaa
       61 acagatgaaa gatcaggttt tattagatct cattctttca tcacttgaag ggatttaatg
      121 aactactttt tcctctttgt cgtttttata tattcgtatt ccttcaaaca gtaggatagc
      181 ctctttcaat gcttgatatt tgaactgtcc gttacttaaa aatttagatg tgccgccctc
      241 ttcctagaac atattctttt ttactttttc attaattgac agaccaatcg aacgaaactg
      301 taaatttcga taggataatg attggtcaaa ctcattcatt ttcaagacat ctcgattcat
      361 attcaagata ggaatctttt tatcaacatc actttattcg tattttcctc taccataaaa
      421 gcttcacgct ccacaaaatt gttttataca ctactattca gcccattcaa cgaccaatga
      481 atttt
//
LOCUS       DAZKCC010000238          473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000238 DAZKCC010000000
VERSION     DAZKCC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.251"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>473
                     /locus_tag="ACG8VH_003116"
     CDS             <1..>473
                     /locus_tag="ACG8VH_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFP9111570.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DAZKCC010000239          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000239 DAZKCC010000000
VERSION     DAZKCC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.252"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG8VH_003117"
     CDS             <1..>468
                     /locus_tag="ACG8VH_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFP9111571.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKCC010000240          457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000240 DAZKCC010000000
VERSION     DAZKCC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.254"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..107)
                     /locus_tag="ACG8VH_003118"
     tRNA            complement(34..107)
                     /locus_tag="ACG8VH_003118"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Ile,seq:cat)
     gene            complement(274..>457)
                     /locus_tag="ACG8VH_003119"
     CDS             complement(274..>457)
                     /locus_tag="ACG8VH_003119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111572.1"
                     /translation="IKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLNVFLNAKSMQK
                     ILYFSTFLSLTKIKKR"
BASE COUNT          153 a           76 c           88 g          140 t
ORIGIN      
        1 tctaacacag aacgattcaa ttggctacat taatgtaccc tgtaggactc gaacctacga
       61 ccggacggtt atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac
      121 ataaagccat aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct
      181 tggagtgtga ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga
      241 gacagataaa catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga
      301 gagaaaagta gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa
      361 acatttagaa atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac
      421 ttttgttcgt acactctttt tgaggtgaac ttttatg
//
LOCUS       DAZKCC010000241          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000241 DAZKCC010000000
VERSION     DAZKCC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.255"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..270)
                     /locus_tag="ACG8VH_003120"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="ACG8VH_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          150 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgctat cgttgcgaa
//
LOCUS       DAZKCC010000242          446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000242 DAZKCC010000000
VERSION     DAZKCC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.256"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..398
                     /locus_tag="ACG8VH_003121"
     CDS             141..398
                     /locus_tag="ACG8VH_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111573.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          197 a           61 c           62 g          126 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactc
//
LOCUS       DAZKCC010000243          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000243 DAZKCC010000000
VERSION     DAZKCC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.257"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..437
                     /locus_tag="ACG8VH_003122"
                     /pseudo
     CDS             1..437
                     /locus_tag="ACG8VH_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DAZKCC010000244          430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000244 DAZKCC010000000
VERSION     DAZKCC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.259"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>430
                     /locus_tag="ACG8VH_003123"
     CDS             <1..>430
                     /locus_tag="ACG8VH_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFP9111574.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAZKCC010000245          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000245 DAZKCC010000000
VERSION     DAZKCC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.261"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG8VH_003124"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG8VH_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKCC010000246          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000246 DAZKCC010000000
VERSION     DAZKCC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.262"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8VH_003125"
     CDS             complement(<1..339)
                     /locus_tag="ACG8VH_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111575.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZKCC010000247          414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000247 DAZKCC010000000
VERSION     DAZKCC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.263"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8VH_003126"
     CDS             <1..390
                     /locus_tag="ACG8VH_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111576.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          114 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caat
//
LOCUS       DAZKCC010000248          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000248 DAZKCC010000000
VERSION     DAZKCC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.264"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8VH_003127"
     rRNA            <1..>411
                     /locus_tag="ACG8VH_003127"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKCC010000249          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000249 DAZKCC010000000
VERSION     DAZKCC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.265"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8VH_003128"
     CDS             <1..>386
                     /locus_tag="ACG8VH_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111577.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKCC010000250          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000250 DAZKCC010000000
VERSION     DAZKCC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.266"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8VH_003129"
     CDS             <1..>386
                     /locus_tag="ACG8VH_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111578.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKCC010000251          377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000251 DAZKCC010000000
VERSION     DAZKCC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.267"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>377
                     /locus_tag="ACG8VH_003130"
     CDS             <1..>377
                     /locus_tag="ACG8VH_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111579.1"
                     /translation="FLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGE
                     FKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLT
                     EEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          137 a           66 c           62 g          112 t
ORIGIN      
        1 tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc gagaaacggt
       61 tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat acctagagat
      121 cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa cgatacattg
      181 gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat tgctgatttg
      241 atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat gactaaagtt
      301 ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta tgtcgctatt
      361 tttatggacg ctactta
//
LOCUS       DAZKCC010000252          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000252 DAZKCC010000000
VERSION     DAZKCC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.268"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG8VH_003131"
     CDS             complement(<1..272)
                     /locus_tag="ACG8VH_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111580.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZKCC010000253          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000253 DAZKCC010000000
VERSION     DAZKCC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.269"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8VH_003132"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8VH_003132"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKCC010000254          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000254 DAZKCC010000000
VERSION     DAZKCC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.270"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG8VH_003133"
     CDS             <1..>344
                     /locus_tag="ACG8VH_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFP9111581.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKCC010000255          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000255 DAZKCC010000000
VERSION     DAZKCC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.271"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8VH_003134"
     CDS             <1..>341
                     /locus_tag="ACG8VH_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9111582.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKCC010000256          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000256 DAZKCC010000000
VERSION     DAZKCC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.272"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..>323
                     /locus_tag="ACG8VH_003135"
     CDS             81..>323
                     /locus_tag="ACG8VH_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9111583.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          109 a           59 c           67 g           88 t
ORIGIN      
        1 actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga tttcattcta
       61 aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc acaaaatttg
      121 tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga
      181 cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa gcagatttag
      241 cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc gccacttatg
      301 ggtacattac ctcgaccccg aaa
//
LOCUS       DAZKCC010000257          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000257 DAZKCC010000000
VERSION     DAZKCC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.273"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8VH_003136"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8VH_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP9111584.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZKCC010000258          315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000258 DAZKCC010000000
VERSION     DAZKCC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.274"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>315
                     /locus_tag="ACG8VH_003137"
     CDS             <1..>315
                     /locus_tag="ACG8VH_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111585.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DAZKCC010000259          313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000259 DAZKCC010000000
VERSION     DAZKCC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.276"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>313
                     /locus_tag="ACG8VH_003138"
     CDS             28..>313
                     /locus_tag="ACG8VH_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9111586.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT           89 a           61 c           63 g          100 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata ccaatgtcgc ttaccgctgg
      301 tttttaggct act
//
LOCUS       DAZKCC010000260          302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000260 DAZKCC010000000
VERSION     DAZKCC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.277"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>302
                     /locus_tag="ACG8VH_003139"
     CDS             82..>302
                     /locus_tag="ACG8VH_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP9111587.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRF"
BASE COUNT          107 a           44 c           65 g           86 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 ga
//
LOCUS       DAZKCC010000261          302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000261 DAZKCC010000000
VERSION     DAZKCC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.278"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          107 a           33 c           48 g          114 t
ORIGIN      
        1 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacattc
       61 taatatattc atatgagcta attttatgat taaatggtaa gtttttcctt gtttgtttta
      121 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga tctgtggggt aaatcattat
      181 acaaaagtaa aattttactt attaacaggc tgtagaaata taaaaaagaa ctaaaattta
      241 actaaattat gtgctattct taaggaaagc caagtgtatg agaaagtttc tgagaatata
      301 at
//
LOCUS       DAZKCC010000262          283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000262 DAZKCC010000000
VERSION     DAZKCC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.279"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..283
                     /locus_tag="ACG8VH_003140"
                     /pseudo
     CDS             1..283
                     /locus_tag="ACG8VH_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DAZKCC010000263          283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000263 DAZKCC010000000
VERSION     DAZKCC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.280"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          117 a           31 c           50 g           85 t
ORIGIN      
        1 tctaaatgat tcctttaagg tgtcactaaa aattataaaa aatagtgttg acgcactttt
       61 gatgtcatgt tataataacg aagtcgtttg gatgagatag ctaataaagc ttgattttat
      121 cgaaaaaaac ttcgaaaaag ttattgacaa ataacaataa agatggtatg ataatcaagt
      181 cgctgctaat gagtggcaaa gacgtttaga aaaaataaac aaagaagttg ttgacattct
      241 cttctgaatc tgataagatt aaagagttgt taaaacaaaa aga
//
LOCUS       DAZKCC010000264          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000264 DAZKCC010000000
VERSION     DAZKCC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.denovo.281"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACG8VH_003141"
     CDS             complement(32..>252)
                     /locus_tag="ACG8VH_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9111588.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAZKCC010000265        20407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.049, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000265 DAZKCC010000000
VERSION     DAZKCC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.049"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..1110
                     /locus_tag="ACG8VH_003142"
     CDS             214..1110
                     /locus_tag="ACG8VH_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9111589.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1260..2201)
                     /locus_tag="ACG8VH_003143"
     CDS             complement(1260..2201)
                     /locus_tag="ACG8VH_003143"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9111590.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2327..3448
                     /locus_tag="ACG8VH_003144"
     CDS             2327..3448
                     /locus_tag="ACG8VH_003144"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9111591.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3706..5208
                     /gene="eat(A)"
                     /locus_tag="ACG8VH_003145"
     CDS             3706..5208
                     /gene="eat(A)"
                     /locus_tag="ACG8VH_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9111592.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5275..5802
                     /locus_tag="ACG8VH_003146"
     CDS             5275..5802
                     /locus_tag="ACG8VH_003146"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9111593.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            6013..7074
                     /locus_tag="ACG8VH_003147"
     CDS             6013..7074
                     /locus_tag="ACG8VH_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9111594.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7077..7973
                     /gene="murQ"
                     /locus_tag="ACG8VH_003148"
     CDS             7077..7973
                     /gene="murQ"
                     /locus_tag="ACG8VH_003148"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9111595.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7987..9969
                     /locus_tag="ACG8VH_003149"
     CDS             7987..9969
                     /locus_tag="ACG8VH_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9111596.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10034..10480)
                     /gene="nrdI"
                     /locus_tag="ACG8VH_003150"
     CDS             complement(10034..10480)
                     /gene="nrdI"
                     /locus_tag="ACG8VH_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9111597.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10691..12115
                     /locus_tag="ACG8VH_003151"
     CDS             10691..12115
                     /locus_tag="ACG8VH_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9111598.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12296..13069)
                     /locus_tag="ACG8VH_003152"
     CDS             complement(12296..13069)
                     /locus_tag="ACG8VH_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9111599.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13066..13971)
                     /locus_tag="ACG8VH_003153"
     CDS             complement(13066..13971)
                     /locus_tag="ACG8VH_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9111600.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14125..14445)
                     /locus_tag="ACG8VH_003154"
     CDS             complement(14125..14445)
                     /locus_tag="ACG8VH_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9111601.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14919..15605
                     /locus_tag="ACG8VH_003155"
     CDS             14919..15605
                     /locus_tag="ACG8VH_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP9111602.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15607..16551
                     /locus_tag="ACG8VH_003156"
     CDS             15607..16551
                     /locus_tag="ACG8VH_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP9111603.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16750..17394
                     /gene="pcp"
                     /locus_tag="ACG8VH_003157"
     CDS             16750..17394
                     /gene="pcp"
                     /locus_tag="ACG8VH_003157"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP9111604.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17473..18750
                     /locus_tag="ACG8VH_003158"
     CDS             17473..18750
                     /locus_tag="ACG8VH_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111605.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            19008..20225
                     /locus_tag="ACG8VH_003159"
     CDS             19008..20225
                     /locus_tag="ACG8VH_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9111606.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
BASE COUNT         6556 a         3661 c         4297 g         5893 t
ORIGIN      
        1 aatatttaac aatgactaat gataacaaat tttcagacaa ttacaactat tcagaaaact
       61 tttaggcgaa ttttaataga agaatgattt atttcatcat attttcatac aaatgttaaa
      121 ttttcataca aatataggaa acgttttact ggctttttac tatttaaaca ggctatcata
      181 ggaaaaagtg acagagaaag tgaggcggaa aagatgaaca aaaatgacgt actgctagtt
      241 gcaaaaaata aatcacttta ttttttgcct tccatccgct ctttagatgt ttggtccgga
      301 aaagatttac tacagtcgga attcattaaa tcagctagac aacagttggc aaattatcag
      361 ctgatcgttt ttttagatta tggatttgat atggagatgg ctcgacgcgt gcgcccattt
      421 accaatgcaa aaattgtgtt atttttttgg aatcacttca aagcagaaca cgaggcgatg
      481 ctgaagcagg cacaagatga accggcaatc gatgagattt atcattttga ttttttggaa
      541 gcaaaagaac ttgggctgaa acataatagt tcattttatt cgaaacatat gaatttttct
      601 tctagcgaac caaagacgga tctatttttt ggtgcaacga ataatgggag aaaagaacga
      661 gcagaaagct ataagcagga atttataaaa agagatgtta cgaccaacta tttcattctt
      721 ccacatcgag ggaatgaaca acctggatat cttacttatg aagagtacct gaaaaaaaca
      781 ggggaatcgc gcgggattct ggaactgttg cgggagggtc agcaaggagt gactttgcgg
      841 acatttgaat ccatgtattt tcaaaagaaa ctagtgacgg ataatcaagc gattgctcat
      901 tatcattttt atcgtcctga aaatattttt ttgcttcgag aacgtgattt agatgaatta
      961 ccggaatttc tgcatacacc atttaagcca atcctatcag agctattgga attttatgat
     1021 gcagaaaatt gggcaaaacg atttatcact aacgattcca ctatttatga gcaatacgaa
     1081 tatgacctct cctctgttga aaacaaataa aaaacctaca ttttgaagag attatgtact
     1141 gaaacaaaca agcacaattc ttatggcatc atgagagttg tgttttcttt ttgatctttt
     1201 attgggctta aaaaaagaga accagctgct gattcagctg attctcatca gagagccttt
     1261 tattctgcgc caaggattcc ttcttttgca tggactaatt gggtcaaaaa ggcacaatat
     1321 ggtgtagcaa tatcatattt ttgtcctttt cgccaaatgg caccattgat gtagtcgatt
     1381 tcagtcaaac gatggttttt gatcaggtct tggtacatag atgggtaatg caacccgatg
     1441 ttgtcaggat cataagtaga agcaatatgt tggtacactt cttcttgatc tagtatgatt
     1501 ccttcttttg ctgcgataga agcaaattca gatacgattg ttcgaaccat gctctctgcc
     1561 gctttttgcg cgcctagttc agcaatatta caatccaaaa tcgtgcataa accattcaat
     1621 gttccattta cacaagcttt tcgccaaatg gaataacgaa cgttatggct atagatcgga
     1681 tttaaatttg cttcttgaaa gacttctaca actttttttg taaatgcttc gccttctggt
     1741 tccaagttct ctagttcgat ttctccatca cctaacaatg tgacttttcc gggaccagct
     1801 agaccagctg tccacatcgt gatcccaaat aagatatttt tctttggcac atatttttcc
     1861 agtacatctt catgacctaa tccatttagt aaacacaaaa cataagtatt gtcagtaata
     1921 atggattgga tcgaacaaaa catatcatct aattgattgg cttttgttaa tgctacgatc
     1981 aagtccacgt gttcgttgct ttcgatgatt tcttctggtg aatagattgg cagttttgcg
     2041 accacttctt taccgttaaa gtcagcaatc aacccatttg tacgaatggc ttcaatgtga
     2101 gcaggccaac gatcgatcaa gaggacctca tttccgcttt gatgtagcat caatccgatt
     2161 cggctgccca ttgctccggc cccagctatt gcgattttca tgttttttct ctcctttatt
     2221 caatgagttc ccttttagta tatagaaaaa tggaagaaaa tagcattgtt ttgtttgaaa
     2281 aaaatgaaaa aaagtgtctt acttaagaag aaagtgaggg atacagatgg atattttggc
     2341 ggcaattgat tcatttaaag gaagtgcttc ttcatttgaa ctaaatcagg cagttctaaa
     2401 tggctgggaa gatcgtaaag gaagaaaaat caatgtgccg attgcagatg gcggagaaaa
     2461 tactgcagaa gcaatctatt acgcgctcag gggaacatgg cgtaccattg atgggatgga
     2521 tctcttattc cgtcagcgaa aaatcagcta tttattgaca acttggcaag gaaaaaacac
     2581 cgcagtcatc gaatctgcac aagcattggg gttagatcta cttgataaac caacggatca
     2641 aactatccga cttgcttcca gttatgcctt aggagaactt attaaagatg cactgcttca
     2701 agaagtagaa caaattattg tcactttagg aggaagcggg tgtagcgatg gtggtctagg
     2761 attactccaa tccttgggtg cttctttaga agggtacaca gaaggaaatc ctttattatc
     2821 tactaaaaaa cttcattttg atcgaagaaa cgttcttgtt tctaaagtaa aattgattat
     2881 cgctgcagat gttaccaatc catatacagg tcaacaagga gctgcttggc aatttggaag
     2941 acaaaaagga ggaatagata cgacgctagc tttcttagat aaacaagcag gaaagattgc
     3001 tcagcaaata aaagatttct gggaggtgga tttagatgat attccaggaa gtggagcagc
     3061 aggcggtatt ggtggagccc tttttttgtt gggagcgaag atggtttctg gtttcgattt
     3121 agtcagccaa atcactggat tggaaaaaaa gatcgaacaa gcagatgtca tcattacagg
     3181 agaagggcgg atcgatcagc aaacgatcca cggaaaagtt ccatacggtg tagcagtgct
     3241 tgcaaaaaaa tatcgaaaac caataatcgc tatttgcgga agtagagacc aagagctaga
     3301 aggactttct gattttctcc caattgtctt aagtattcat ttgggtccaa taggtttaga
     3361 agaagcaatg gaacacaggc aaacattaga aaaaacaaaa atcttaggac agacacttag
     3421 cagatttttc accatcctcc cgaagtagtt gacaaaatca ttttctttat gctatcgtaa
     3481 tttcaaatat aggacaaagt gagggatccg tgcattgaaa aattaagtcg attgatgaaa
     3541 gctgcattca gactgtacct tgtggagaca aggaagcagt gtgcagattt tcgggattgg
     3601 cttggttttt tcgatgtatg ggtctttttg tgtgtatcca acccttgcat cctctttagc
     3661 cagttctgaa aataggactg gtcttttttt acgaggtgat ggaaaatgtc taaaatcgaa
     3721 ataaaaaatc tgacattcgg ctacgacagc caaggcacat tattatttga acaagcaaat
     3781 ctaaattttg acacacaatg gaaactagga cttatcggac gaaacggtcg aggaaagaca
     3841 actttactga atattctaca aaacaaacta ccttatcaag ggcaagtaat ccatcagcaa
     3901 gaatttgcct atttcccgca acagacaaaa gataaagaac gtttaaccta ttacgtgtta
     3961 aatgatatta cggattttga gatatgggaa atcgaaagag agctccaatt gatgcaaaca
     4021 gatcctgaaa tcttatggag agaattcagc acactatcgg ggggagagaa gacaaaagtc
     4081 ctactggcac ttttatttgt ggatgacact catttcccgt taatcgatga accaacgaat
     4141 catttggata tctctggtag aaaacaagta gcggcttatt tgaaaaagaa aaaacaaggc
     4201 ttcatcgtgg tcagccatga ccggggattt atcgatgaag tagtggacca tgttttagca
     4261 atcgaaaaaa gtcaactgga actttatcaa gggaatttct ctatctatga agaacagaaa
     4321 aaacttcgtg atgaatttga aatggctcaa aatgaaaaat tgaaaaaaga agtcagtagg
     4381 ctaaagaaaa cagcagctga aaaagccgaa tggtctcgtt cccgagaagg agataaaaca
     4441 aagaaacaag tcggattcat cgatactgaa tctagacgag tgaataaagg agcagtgggt
     4501 gctgatgctg cacggacgat gaaacgatcc aaagcaatcg tgaatcggat ggagacccag
     4561 atcagcgaga aagaaaaact attaaaagat atcgaatata tcgattcttt gacgatgaat
     4621 agccaagcgt ctcaccataa gcgactttta agcgtagaag atcttcaatt agggtatgaa
     4681 aatctgttat tcgagccaat tcattttaca atcgagcctc atcagcgggt ggcgatttca
     4741 ggtcctaacg gtgcaggaaa gtcatccatt atccattatc ttctgggggc attcaacggc
     4801 aaggttatag gagaaaaata ccagccaaaa catctgagca ttagttatgc aagccaaaat
     4861 tatgaagaca atcgaggaac gttggcggaa tttgcagaga aaaaccaagt agactaccaa
     4921 gcatttttga acaacctccg aaagcttggg atggaaagag atgtttttca taacaagatc
     4981 gagcagatga gtatgggcca acggaaaaaa gtggaattgg ctaaatcttt atcacagcca
     5041 gctgaactat atacatggga tgaaccattg aattatttgg atgtcttcaa tcaagaacaa
     5101 ttagaacaac tgatcttgaa cgtgaaacct gccatgttac tagtggaaca tgatcaaacc
     5161 ttcctggata aagtatctac tgagattatt tctcttgaga gaatctaacc cttgtaatct
     5221 caagaacaac aaggtatggt taaggggaac tttgccaacg aaggagagaa tcacatgctt
     5281 tatgtaacga gacacggaga aacaacttgg aacgcacaag gattagtttg cggacgggca
     5341 gatgtgccgc tgactgaaaa aggacagatg caggcacaga aactagcaga aaaggtagta
     5401 gatttgcctg ttccaatcac gaaaatcatc cattcccctc ttcaacgagc aagagataca
     5461 gcacaagcag ttgctgatcg tctcagcttg ccgctgaccg ttgatgaacg attggtagaa
     5521 atggattttg gggattatga tggactgcca agcaaagatg agaactttca aaaagcacgt
     5581 ttagcgttcg ctgtacgatt cccaaatggg gagtcagtct tagatgtgta tgcacggatc
     5641 gttcctttat tgaaagaatg catcgaagac gaagaaaatg tctatctgtt agtttgccat
     5701 aatgccttga taagagtgat caatgcttat tttcatccga tgcctaatga ggggttcttt
     5761 acattcatgg tagataatac agaattggtt tcttatgagt aattatgttt ttgtgttatt
     5821 taaataaaat atttcaaggc actgcttttg cttagcagtg ccttttttag gttatgacaa
     5881 ttcttttgat aatataaact caacctttag aaatgaaatt taattatttt aataaaagta
     5941 ttgaaatttt gtttctttag atatatactc tagtcataga aatgaagacg gatacagaaa
     6001 aagagggata atatgtttgg cttttcaata tttatgaacg attctttgtc agaagagaag
     6061 aaacagtata tccgccaaat ggcatccaat ggctttgtcg gtattttcac ttccatgcat
     6121 ataccagaag atgatgtttc ggcatatcaa aagcgattga tcgaactagg aagcgaagcg
     6181 aaaaagaata aactagaatt gatggtcgat atttcaggag aggcattgga acgagcgggt
     6241 ttttcattag aagatctaac gcctttgaaa gcaattggtg taacaggttt aagaatggat
     6301 tatcatattt ccaatcagca gattgccgac tggtcacatc aattgaaaat cagtttgaat
     6361 gctagcacga tcacgcctaa agatatcgat gaattgaaag aagcagaagc tgatttctca
     6421 cagattgaag cttggcataa ttattatcca cgtcccgaaa cagggctgga caaagaatgg
     6481 tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc ttgtccaagg ttttgtacca
     6541 ggagatacag agttgagagg cccactatat caaggattgc caactttgga agaacatcga
     6601 gggatccatc ctcttgcagg cgctctagat ctattagcaa gtaacacgga tattgtttac
     6661 atcggagatg caggtctgtc agaaaacgtt caggagcaat ttgcttcttt ccaaaaagaa
     6721 cagacagtct tgctccatac ggaacctgta gatgaagaat tttacgaata tatattaggg
     6781 aagcatacga atcggcaaga cgatgcaaga gatgtcatac gaagcgtaga tgcacgtttt
     6841 cgagagattc cgcctatccc agcaagaaat acagctacgc gtatgaaagg gtctattact
     6901 ttagataacg aaaagtattt gcgctatatg ggtgagatcc aattaaccaa atatgatttg
     6961 ccagcggatg aaaaagtgaa tgtagtagct aaagttatca aagaagagct accattgatc
     7021 aaccaaatca aagctggtat gaactatcaa tttataagaa aagaaggaag ataaaaatga
     7081 gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa ccaagaaact tttggattag
     7141 atgagatgag tatttctcaa gccgttcaac taatgaacaa agaagatcag aaagtggccg
     7201 ctgcggttga aaaagaactg acaagtatcg aaccagtgat tgctaacacg attgagtcct
     7261 ttaaaaaagg tggacgtctg atttatatgg gagccggaac aagtggaaga ctaggtgtgt
     7321 tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc ggaaatggtc ataggtctaa
     7381 ttgctggtgg agaagaagca atgactgtag ctgtcgaagg ggccgaagat tcttatgagc
     7441 tagggcaaga agatctaatc aagctgaatt tgacaaaaaa tgatatagtt attggtatcg
     7501 cagctagcgg tcgtacacca tatgtgatcg gtggattaga ttacgcaaaa tcgatcggtg
     7561 ctgtcacagg aacgatttct tgcaataaac aagccgaaat cagtaaacac gcggatttcc
     7621 caatcgaagt cgatgcaggt ccagaatttt tgacaggctc tactcgattg aagtcaggaa
     7681 cagcacaaaa acttatcttg aatatgattt ctactatcag tatgattggt ataggtaaag
     7741 tattcaataa cttgatggtc gatgtcaaac caacaaatga aaaactaatc gaacgctcaa
     7801 aacgaatcat catgcaagcg acagaagctg attacgaaac agctgaaaag tactttgaag
     7861 aagcagatca aaatgttaag ctggctatcg tcatgatttt gacaaatagt gataaagagg
     7921 aagctcaagc aaaattagct gaagcaaacg gatttgtaaa agggacctta tgagggggaa
     7981 aagaccatgg caaaaaacga actgactttg gaagaactgg caaaacaaat ttataaaggg
     8041 gcaggcggca tgggcaatgt agaaagcatt gtccactgca tgactcgtgt gcgtatgaag
     8101 atcatcaatg atgatcttgt aaatgtctct gaattaaaag cgattccagg agtgcttggt
     8161 gtagtagaag atgaacagct tcaagtcatc atcggacctg ggaaagtgaa caaagtagca
     8221 cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa aactttcttc tgtaagtaca
     8281 gctacagcaa attcatcagc atctggaaaa gaccgtgtaa acgaacgggc acaagaaatg
     8341 aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca aatcggtatt aaaagacatt
     8401 tctaatatct ttgtgccaat gattcctgca tttgtcggta ctgggatcgt ggcagggatt
     8461 gccgcagttt tagcaaactt agtcacagcc ggaacacttg atgcagcaac atggcaacaa
     8521 tatatcgatg tgatgaacat cttgaaaaac gggatgttta gctacctagt tatctataca
     8581 ggtatcaatg ctgcacaagt ttttggtgca acaccaactc ttggtggagt tatcggggcc
     8641 gttgtgatgc tgacagggat gaatccggaa gcaccactga aaaacttatt tactggtgaa
     8701 gctcttgcag ctggacaagg tgggatccta ggtgtcatct tcgctgtttg gctattgtca
     8761 atcgttgaga aaaaactgca taaagtagta cctgatgcgg tagatatcat cgttacacca
     8821 actctttcat tgattgccat cggtctgatt gaaattttct tcatcatgcc tttagcaggt
     8881 ttcatttctg atggcatggt cggtgggatc aactgggtac ttggcgttgg tggagcattt
     8941 gccggcttcg ttctaggcac attattcttg ccgatggtta tgttcggact gcatcaaatt
     9001 ttgactccaa tccatgtgca aatgattgat gaaactggtc gtacgctatt gcttccaatt
     9061 cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg cattatggat tcgctgcaaa
     9121 aaagataaag aattaacaga aatgatcaaa ggagctttac cagtcgggat cctaggaatc
     9181 ggcgaaccat tgatttacgg agtaacatta cctctaggac gcccatttat tactgcttgt
     9241 atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg gaaatgtcgg ggctatcgct
     9301 attggacctt caggagccgc tttgatccca ttgatctctg acggtaaatg gttaggctac
     9361 atccttggat tattggcagc ttatgctggt ggcttcgttg ctacattctt cttcggtatt
     9421 cctaaagacc gtttagaaaa agaagaatta gcgactgaaa cagccacagt aaatgaagtg
     9481 cctgaaaaag tagtacctca aacttcagct gaaagtgtga ctatcctctc atcagttgct
     9541 gacggaacag ttgtttcttt agataaagca agtgatccag tttttgcaca aaagatgatg
     9601 ggtgaaggct actttgtaga accagaaaac ggacatatct attctccagt tactggggta
     9661 gtcagctctg tcttcccaac gaaacacgct attgggatca caacgccaaa tggtttagaa
     9721 atcttgcttc atatgggaat caacacagtt gacttaggcg gtacgccatt tgatgtgaaa
     9781 gtaacagaag ggcagcaagt gacacctgat acgcttgtag cagaagctga cttagaagcg
     9841 atcaaagcag caggcaaaga aacatccatg atcgtcttgg taaccaatat ggatcgtgta
     9901 aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa aagcacctgt gatggatgtc
     9961 aaagcttaat atgatagaaa caatagaaac gaaagaagaa actgaaatct atttttcagc
    10021 ttcttctttt ttattattta ttgttttctg ctaaaatctg ataaattcgt gtcacatcag
    10081 atggtgttcc ccgtaattca taatctgcca agaaaggaaa gccaaatgtt tgtgcatatt
    10141 gtttggctgt tagacaatac tgattattga agtttttatt tccgctaccg actacaccaa
    10201 gacaaagttt atgattgtct tcatgttcca gatattcacg catcgtttcg gtcaagattt
    10261 ctgtatcacc gttatctaaa ccatttccgc cgtctaaata agtaggaacg aaagtaaaaa
    10321 atggttctgt ttctttcgta aaatcactat tttcatggat ttcttttaaa gtaacttcag
    10381 gattgacagg atcttctgca tgcatttttt cagcatattc tgctagatgt ttggcaaagg
    10441 cacgcgtatt tcctgaaatc gatatatata aaatgttcat ggatcatcct ccaattcttt
    10501 ccttttagta tagcatcttt tttaagtttt ttggtggtgt aatttggaaa gggactaggc
    10561 gctttgcttg tataatgaga aaaaagccgg tagaattata tttctattaa agtttttatt
    10621 aattaagtaa aaataaaaaa ggtttgttat actttttgat aaaagcggtt ttattttgga
    10681 ggatgcttac atggatacag agcaacagaa aaaaggaatt ggtttaattc cacttgctgc
    10741 cttagtaatt ggttcagcga ttggaggtgg cgtatttggt attatgacgg acatctccgg
    10801 atcggctggc ggcccagcta ttttcagttg ggtactcgta ggaacagcaa tgttgatgct
    10861 ctccttgtcg atcaataata tcaataaaaa acgtccagat ttaaaaggcg ggatctttag
    10921 ttacgcagaa gaaggttttg gccgtttcgc tggttttatc agcggttggg gttactggct
    10981 cacatgctgg ttaggcaacg tagcttttgc aacattgttg atgagcgcat taggatattt
    11041 cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc gtgatgagta cagtcttttt
    11101 atggggatat gcatggctag tgaatcgggg agtcgaaaat gctagtttcg tcaatgctat
    11161 cgtcacattc tgtaaattag tgccattatt tttatttatt gtagtagcta tcagcacgtt
    11221 ttcgatccaa caattcattg ataacttttc tagcgacatt atattgaatg cgaatggaca
    11281 gccgatcccg ttttccagtc aagtattgag ttgcatcatg gtgatgcttt gggtatttgt
    11341 tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa aaacaatcag atgttgggaa
    11401 agcaacgatt ctagggatat tcggcttgat tattatttac gtcttggttt ccatgcttcc
    11461 atatgggaca atgacgatga atcagctgca aacgatcaag gcgcagcctg ctatgggcta
    11521 tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg atcaacggcg gtttgatcat
    11581 ttctttagtt ggtgtttggc tctcatggac gatcttaccg attgaaacaa tgggaaacat
    11641 ggctgaagac ggacttttgc ctaaagcatg gggaaaactc aacaaaaatg gggcaccaac
    11701 ttatgctatc gtattgacga cgttatgtac gaatgtcttt ttactttcat tattattcac
    11761 tgacagtgct tataactttg cttacacact tggtacagca gctattttct tcacgtggct
    11821 tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg aaagaatggg tccaattttt
    11881 agtagggctt ttagcaagtg cctttcaagt atgggctatg ctagtcgtag cctttaatga
    11941 agtaatgctt gttcttgttc ttttcttgcc aggcatttat ttttacctca aagcacgtaa
    12001 ggagcaaaac gagcctgtac aacatatcca cttagataag attttactta taggtgtgac
    12061 gatggtcgga gtgattgcaa tggttttatt tgccactggg atgcttcacg tatagaagta
    12121 taaaagaagt ataaagagag gttgtgagcc tgtcgtttat tcaaaaacat actaggaaag
    12181 gagctgtgac acaaatgttg tcacagctcc tttttcagaa taaacggtgt ttaagagaca
    12241 gccaatcggc aataaggctg aacgtctctt tcacagcctc ttttttgatg agtcatcata
    12301 cattttttcg cttaaatacg agatatccta atgctaagaa caaaacgatg taagctaaat
    12361 taccgaatag catttgatta gtagaaagat gagtcaattg cttcatgatc tcttcgtttc
    12421 caatttggtt ttgcagattc aacatactga tcggattcca tttgatccac tcccattttt
    12481 ggatgagtgc aataaggata ccagatatga ttgacgaagc aaaataaaag acgattcctg
    12541 ctgagatgga tgcgccggaa ctattgataa agcaagcaat catcaacact aaacttaaaa
    12601 tcagccataa accgatataa ctacccaaag tataagtgaa caagctttgg atcagtgttt
    12661 gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa aagaatcagt ttcatcaaca
    12721 cagtgacaat aatagctaaa aggtataaat aaacagaata aataactaat gtgatccatt
    12781 tgcttactaa tatttctcct cgagaatact ttcgataaag gagatttttc aatgtaccat
    12841 attgtgcttc catcgaaatg atcgtgcttg cagctgcaat catgataaag acaatccaag
    12901 atgtggcact aaaaagctga gcagtcattt caactggatc aatgatcgtc gtgtattttt
    12961 tggctagaag tgctgttcct atgagcaaaa caactaatag aacagataaa acagcagttg
    13021 actttttctt gatcaattta aataattctt gttttacaag caatgtcatt tttcttcttc
    13081 cccctttact gaatccagaa tttccaatag agaagattcc aaatcattcg tcagatgctg
    13141 gacgtctgtc acatggatat tttgtgcaga caatagctgg atcacggttc ctaataattc
    13201 ttcgttttct tttaaaagct tgattttatc aggatcttcc aaagtatatc cagcttctgt
    13261 caagatcgtt cgagcagcta gatcatttgt tgtagagata acgatgaaat cttgcgctgt
    13321 ttggttcaat tcagccattg tacattgttt gatgatcttt ccttgattga tcaggatcag
    13381 ctcatcgacc attttttcaa gctcgctgag taagtgactg gagatcaaga aagaggtgcc
    13441 ctgagtagcc aagtcggtaa tgatttttcg cacatctctt gttgcttgtg gatccaaacc
    13501 gttcatcggc tcatctaaaa taacgataga cggatggttc agtaaggcaa gagcaattcc
    13561 caatttttgt ttcattccca acgagtagga tttcgctttt ttgtgaatgt acttttccat
    13621 cttgagctga ttcacgatag tgtcgatagc agatacatcg ttggtttcag agaataactt
    13681 cagatgatca tagcctgtta agaatggata gatacccggg tattcgatca atgctcctac
    13741 ttgttccaac cctttatgac tagagggaga aatctcattt tcaccaatac agattgttcc
    13801 atctgagtaa tggatcagtc ctaaaatagc tttcatgatc gttgtttttc cagcgccatt
    13861 aggacctact aatccaacga ttttgccagg ggataagcta aaactgacat cgctgagggc
    13921 ttgtttgtga ccaaagttct tggataattc ttggaccgaa agacttttca aaaatattca
    13981 ctccttttct atagatagta caagcataca cctgatgagt ggagaagtcg ctaggtcatt
    14041 ggacttattt cttatttttt cataaaaaaa ttgaatatta aataaaaagg cttatttttt
    14101 ctgatcagta cttattccaa gtgtctagtg gccgctagta atcttgatgc caatgatact
    14161 gatgagtagc atccccacaa agaaccaagt ggaaagattc agcttatcat gaaacaagac
    14221 cacaccaatt atgaccgaac caactgcgcc aatccctgtc cagataggat aagagagact
    14281 catcggcaaa cttttgattg cctttgacaa aaagtaaaaa ctagcaatca tgccaatcac
    14341 tgtgtaaaaa gaataattga ttttagaaaa accatcactc catttcatca tcgttgacca
    14401 gaatacttcc aaaattcctg ctatgattaa ttccatccat gccatactta cattcctcct
    14461 taactataaa aaaatacaca aaaagaggaa aatcatccct tcaaagacct aatggaatga
    14521 tcatcctctt tatcctaccc ggtggcagat tgtatatctt tttctgtagt atagcacacc
    14581 tatccagata tcggcaatct catacttttt agtaaagtgt accaagcact gacggatata
    14641 tcgttagcgg aaaagaagat gaatgctttg caatttcata gaaagaagtt gttatttatt
    14701 aatcatttaa aaaaaacagt aactagacct tatttctata tattatcggg caataaatta
    14761 aaaaaagctt taccttacaa taagtaagat aatttttgtg ataaaaaaat aacgaaaaat
    14821 atttaaaaat agtgtctatt tcgtgagaaa acaactatat tttttatgtg ttttgcttta
    14881 taattaaaaa aatattgtca ttttatggag gaaaatatat ggaaaattat cttagccttt
    14941 taggcgtctt gattgttatt ctcgggtttg catttaaatt agactcgata ttgatcgtaa
    15001 tggttgcttt agttgttaca gctttgactg gcggtcttgg cattcaagga atgttagaac
    15061 ttttaggtac aagttttgta aacaatcgcg ggatggcgat ttttatcatc attatgttgg
    15121 caacaggaac gcttgaaaga aatggtctga aagaatcagc ggcttccttg atcaagcgat
    15181 tcaagaaagt atctgctggt atgattgtag atatttatgg gatctttcga atgatctttg
    15241 cggcttttaa cgtgagtttt ggtggagttg ctggatttgt tcgtccaatc attttaccga
    15301 tggcattggg gaccatcgaa tcaaaagggt tagaagtcca tcctaagcat gaagaagaac
    15361 taaaaggtat ggcttcagct atggaaaatg tctgctggtt ctttggccaa gtactattca
    15421 tcggaggagc aggggcgttg ctagtccaat caacactgaa agatctagga tatgacgtaa
    15481 cattgggaca gcttgcttta gtggaagttc cagtggctat cacggcttta gttgtggcaa
    15541 gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa atattatcca ggaaaggggc
    15601 aataagatgg acttttttac aaatcctgat attttattag gcgataaatt actggagatc
    15661 ctttatatcg tgatgggact cgtattgatt tacactggag taagaaattt aacagataaa
    15721 accaatcctc atcgttatgg gtcagccttt ttctggacgg tcttaggggt agttattgca
    15781 ggaggaagat ggcttccggc aatcgtgaac ggtggcttga tctttgcgat gaccatacca
    15841 gcaattgcta agaaagtcag caaaggagaa agccggcttc catcaaaaga atatatggaa
    15901 aaaatgtcag acaaactagg aatgaaaatt tttattccag ctttgagtat cggtgtcttt
    15961 gcgatcttgt ttgctctatt taccaattta ggtgctttag tcggcgtggg cgtcggtgtt
    16021 tttgcagcca tgctaatctt gatgttcttt tctcgagata atcgaccaag tacattttta
    16081 gatgatgcag cagatatgct gggcacagta gggccgttaa gcatgttgcc aatgctttta
    16141 gcttctctag gtgcggtatt tacaagcgct ggagttggga cagtgatttc caatgcagta
    16201 ggtacgatcg ttccagaagg aaatgtgaac atcggcatca tcatttacgc tcttggcatg
    16261 gtgatattta cggtaatcat ggggaatgca tttgcggcca tcaccgtgat gacggttggg
    16321 atcggtgcac cattcgtatt tagtcatgga gcgaatccag cgttaatagg aatggttgca
    16381 ttgacttgcg gtttttgtgg gaccctgctg acaccgatgg ctgctaactt caatatcgtg
    16441 cctgtagcga tgctggagat gaaagacaaa tacggagtga tcaaaaacca gttgtttatt
    16501 gcgttattta tgctagtttt ccaaattgct tatatgattc tatttaaata atagaagaga
    16561 ggggttgtga cagaataatt gtcgcaacct cttctaacat tgtagtggaa cactattttt
    16621 ttataagaat atattaagtt tgatctgtta tgttatttaa actattgttt cctttattta
    16681 cgctgttcaa ggaaaatggt atgataaaag ataaccatat gttaaatcaa tcggacggag
    16741 gaacaacaga tgaaagtact agtaacaggt tttgatccat ttggtgggga caaagtgaat
    16801 ccagcctatg aagcagtaaa aaaaatgcca gatgaaatca gcggtgctga aatcatcaaa
    16861 gtggaagtac cgaccgtatt cgaaaaaagc agtcaagtag tcaaagaagc catccaacag
    16921 caccaaccag atgtcgtgat ctgtgtcgga caagcaggtg ggcggtcagc agtctcgttc
    16981 gaacgtgtcg caatcaatct agcagaagca agaatccctg ataatgaagg aaatcagcca
    17041 ttcgatacgg ctttagaaga aaacggtcca gccgcttact ttacctcatt gccaatcaaa
    17101 gcaatgacaa aaaatgtaca agatcatggt ctaccagcgt acatctctta tacagcagga
    17161 acatttgtct gcaatgatat catgtatcgt ttgcttcatt tgatcgaaac acaattcccg
    17221 gcaattcgag gtgggttcat ccacgtgcca ttctcacccg atcaagtgat cgatcgtcca
    17281 gtgggtacgc cttctatgtc tttagaagac attgcttctt ctttgactta tgcagttgaa
    17341 gcagcagtag aaaataaaga agatatttcc ggaaatgccg gaacaactca ctaatttagc
    17401 aagaaacact ggctggttgc taacgttatg ttagtagcca gctttttgta attagaaagg
    17461 aaagaaacag ccgtggaaga atggcaatcc gtctttgaag aatggtttcc aaaagaaatc
    17521 agcaaaagct atcccatcaa aatttctaaa caatacacaa gcagtcaacg ttgggagatc
    17581 tatgcaaaat taacgaaaaa acagcgggaa ctagtcgata agcaccgccg atatctaatc
    17641 agctctcgtt ttatggaaga acattattta gctgcaacag attgggtgtt ttctgacttc
    17701 aagatcaatc cgttttttcg aacaaaacga agccagcaaa aattgtattg tgagtgtggt
    17761 agagaattaa aagtgcaata catcgttaaa tcgccaaaaa ccggaaagat cctaaaacta
    17821 ggcatcaacc actttgccga tcacctgcat gtctctccga cagtggcagc ttcgatccat
    17881 cagggaatga caaaagttga tctggcctta gacgaattat tatggcttaa gcagaagaat
    17941 atcgattttc ctgaaggact atggcaaaaa tactgttttg tcttgtacca aaatcgtcgg
    18001 atgaaacagc catatttacc ggatataaaa ttagctcagc ggctcgcaga atttcgccaa
    18061 gtagaaatgc cgatttacat agcggattat caagcattgg aaaatgaaat caagaaaata
    18121 agtgaacaca tcaatgggca gtcaaaaaag cgacagataa aaaaagaact atttgacgac
    18181 tttgcagaag aattagtaaa ggatgtagaa gaattcttga tcaactatcg cgcattttta
    18241 cgtaaagatt ggcaatccat cgtatatgaa gaagtgccag tacatcctaa tgcttatttt
    18301 gaaacattta tttccgtgtt gagaaagaca aaacgtcaac gaacaccaga agtcactgct
    18361 cagatggaat attttgcgaa aaaccaacga ttcatccaac caaaaatcta tctatttatt
    18421 tggaagcaat attgtcgcta tggttttaca gaagggttct tcgacagcat tcctcggata
    18481 gtcagaaatg gttttttaaa agtattgcgt aaagaacgag aagctataca atctgctgat
    18541 aagaaagacc gtacagtctc aaaagaaaaa tggcaactag tagtgaaaga tatccaatct
    18601 ggaaatgtgc aagagacaat cgacaaatgg aaaggaaaac attatcgttt tactgaagct
    18661 cagaaacaag cgttggaata ttatcagaag ttagaagaat cactgcgttt caatgatgag
    18721 gcaagaaagt atctgaagga attactataa aaaacatact aagattagag gggaacatca
    18781 ttgacgaatg atgttctcct ctattttcta tttttagatt atagtgaaga gtgaaagaaa
    18841 gttcgaacaa agccttgagg actacgagtc cctatcaaaa accgaacttc tttactgtca
    18901 ttttagaatc aggtttacgt atgttggggc catgagcccg tgtataggaa attggaatcg
    18961 aaatgaagca gtaaagttct ttcaaaactt aaaaaggagg aaaacaaatg ccttacattc
    19021 ttgcattaga tgtttcaatg ggacacagct acagtgtcct atacaaagac gacacctgct
    19081 tatttgaaga cgagattgaa cacactcaaa gaggatttca tgcattactc gaagagatcc
    19141 agcacttgcc tgagcgtcca ttgattgttt ttgaagcaac ggggatttat tccagaccag
    19201 tcgaaaagtt ctttcaagat catcactttt cttactgttt gttaaacccg ttagaggcga
    19261 aaaaacagat ggaagaaagc accttacgca gttggaaaac agataaatcg gatgcccacc
    19321 ggctcgcaca gactcatctt aaaaatcaac gtcagccaaa agaagtacaa agcaattttt
    19381 acttagagat gcgcgattta gcccgttttt atcaagaaat tgagaagaaa atcactcgtc
    19441 tgcgcatgga tttacacaac tgccttcaat tgacttttcc agagcttgaa cagttctttt
    19501 caaacaggct gaccccttac gcattgacat tgatccgtct ttttccccat ccagattttg
    19561 tattggcatc tactcggaca aaaataaaaa ataaactgat caacgaaaca cggaaaaaaa
    19621 tctccgcgaa tcgtgccgag caaaaggcag atcaaatcat ccactatgca cagtgtgcct
    19681 atccagctgt tgaaaaagac agtatccact gtcaaaaaac gatctattac gccgaacttc
    19741 tccaagattt actcgaacaa aaaaaagcac tggcgacaca aatgatcaaa aaagccgaag
    19801 gctctccttg ctttttgttg taccaaacgt tccctgggat tggcgcactc acagccgctt
    19861 tattgctcgg tgaattagga gatatcacac gatttaaaac ccataaacaa ttgaatgcct
    19921 ttgtcggaat cgacatccga cggtatcatt caggtaagta tactggccaa gaccgcatca
    19981 ataagcgtgg taatcccaaa gcacggaaaa ttattttctt caccattcga aatatgattc
    20041 gccagcaacg cgcagctcct aatcatattg tcgattatta ctacaaatta aaaaagcaac
    20101 ctatccctaa gaaagataag gttgccacag ttgcttgtat gaacaagctt ctgaaatgta
    20161 tgcacgctat ggttagggca catacagagt acgactatgc gtacgcggtc tcagtggacc
    20221 attaactaaa ccacttacag tataccactt ctttgcccag atcgaaaatt ttttacgaac
    20281 ggggctttat ttggtgtgct ttgattttgt cgctttcgtt tacgagctat ttttcttgca
    20341 ccttttactt gactaatcgt aggaaataga ggctgggaca gaagcgttta actccaagaa
    20401 ataagaa
//
LOCUS       DAZKCC010000266        15143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.071, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000266 DAZKCC010000000
VERSION     DAZKCC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.071"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..221)
                     /locus_tag="ACG8VH_003160"
     CDS             complement(<1..221)
                     /locus_tag="ACG8VH_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111607.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLR"
     gene            complement(484..3300)
                     /gene="uvrA"
                     /locus_tag="ACG8VH_003161"
     CDS             complement(484..3300)
                     /gene="uvrA"
                     /locus_tag="ACG8VH_003161"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9111608.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(3310..5304)
                     /gene="uvrB"
                     /locus_tag="ACG8VH_003162"
     CDS             complement(3310..5304)
                     /gene="uvrB"
                     /locus_tag="ACG8VH_003162"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9111609.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            5563..7065
                     /locus_tag="ACG8VH_003163"
     CDS             5563..7065
                     /locus_tag="ACG8VH_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9111610.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            7067..7804
                     /locus_tag="ACG8VH_003164"
     CDS             7067..7804
                     /locus_tag="ACG8VH_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9111611.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7975..9168)
                     /locus_tag="ACG8VH_003165"
     CDS             complement(7975..9168)
                     /locus_tag="ACG8VH_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9111612.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(9247..9453)
                     /locus_tag="ACG8VH_003166"
     CDS             complement(9247..9453)
                     /locus_tag="ACG8VH_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9111613.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(9446..10588)
                     /locus_tag="ACG8VH_003167"
     CDS             complement(9446..10588)
                     /locus_tag="ACG8VH_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9111614.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            11100..11735
                     /locus_tag="ACG8VH_003168"
     CDS             11100..11735
                     /locus_tag="ACG8VH_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111615.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            11889..11966
                     /locus_tag="ACG8VH_003169"
                     /pseudo
     CDS             11889..11966
                     /locus_tag="ACG8VH_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            12280..12327
                     /locus_tag="ACG8VH_003170"
                     /pseudo
     CDS             12280..12327
                     /locus_tag="ACG8VH_003170"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12343..14262
                     /gene="tet(M)"
                     /locus_tag="ACG8VH_003171"
     CDS             12343..14262
                     /gene="tet(M)"
                     /locus_tag="ACG8VH_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9111616.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14381..14548
                     /locus_tag="ACG8VH_003172"
     CDS             14381..14548
                     /locus_tag="ACG8VH_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9111617.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14608..14961)
                     /locus_tag="ACG8VH_003173"
     CDS             complement(14608..14961)
                     /locus_tag="ACG8VH_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111618.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         4458 a         3001 c         2834 g         4850 t
ORIGIN      
        1 aaacgaagca tcgtctcgat aaatacaggg tctttatcaa atcctggaat aacttctaca
       61 ttgacaattt tactgtagcg ggtaggggct aactgatccg aagcatatgc ttggcagatt
      121 ttgccgtatg cttcattaaa agaacgtgcc ataccataaa cgttcatgtc gcttcctaat
      181 aatataggga taaattcttc attttcaata ctgttcatca ttatctctca cttactcata
      241 atctcttgtt tttcttttcc aactacacaa ggaatatcat accataaaga aaaaagcaat
      301 caaaggaaag tcctccgatt gctcaattct tttttgaacg gattgaactt ataggaagtt
      361 tttatctata ccaatcagtg tagaaagaac taccatgtaa tgattctaga acactgatat
      421 taataaaaca agcaaaaatt cattttttta tgttttttta actatctcag ctatctatcc
      481 tctttatacc attatttttt tcaaatagtg acctgtataa ctttctttta cgttaatgat
      541 ttcttctggt gttccagtcg caaggatcgt tcctccacct tctccacctt ctggtcctaa
      601 atcaatgata taatctgctg ttttgattac atctagattg tgttcaatca ctaaaactgt
      661 gttaccagca tctactaatc tttgtaatac atgcaacaag cgggcgatgt catcgctatg
      721 aagtcctgtc gttggttcat ctagtatata gaaattcttt ccattagaga ttttgtgcaa
      781 ttcactggca agtttcatcc gttgtgcctc accaccggac aatgtcgttg ctggttgccc
      841 catcgtcaca taacctaagc caacatcaac aatcgtttgc agtttgcgat gaatctttgg
      901 aatgtgcttg aagaattcta cagcatcctc tactgtcatt tccaaaatat cagcaatgct
      961 ttttcctttg taatgcactt ctaatgtttc agaattatat cgtttaccat gacatacttc
     1021 acaaggaaca tagacatcag gcaaaaagtg catttcgatc ttaataattc catccccgcg
     1081 acaagcttca caacgaccgc cttttacgtt gaaactaaaa cgccctttct tataaccccg
     1141 catttttgcc tcgttcgttt gagcaaataa cccacggata tcatcaaata cacttgtata
     1201 agtcgctgga ttacttctcg gcgtccggcc gattgggctt tgatcgatat cgatgatctt
     1261 ttcgatactt tcgtagccgg aaattgtctt gaatttacct ggcttagcag aattcttatt
     1321 taatttttgc gctaacgatt tctttaagat actattgacc aatgtactct ttccagaacc
     1381 agacacacct gtgactgcta caaattctcc taatggaaat tcaacactaa tatttttcag
     1441 attgttttct gaagcaccag tgatcttgat tgctttccca ttacctttac ggcgttcttt
     1501 tggtacaggg attgattttt tccctgaaag atatttccct gtcaaagaat tttcatcatt
     1561 agctacttct tcaggagtgc ctgcagccac gatttgtccg ccttgttctc cggccccagg
     1621 tccgacgtca atcaaataat ctgctgccca catcgtatct tcatcatgtt cgactacaac
     1681 gagggtgttg ccaagatcac gcattttctt taatgagcca atcagtcgat cgttgtcgcg
     1741 ttgatgcaat ccgatcgatg gttcatctaa aatatacaaa actccagaaa gattcgatcc
     1801 aatttgtgta gccaatctga ttcgctgcgc ttctccccca gataatgttc cggaagcgcg
     1861 actcaaagtt agatagtcta gtcccacatt ttccaagaat gaaagtctgt catttacttc
     1921 ttttaaaatc ggtttggcaa ttactttctc ttgttcagat aacgttaagt ttgagaagaa
     1981 atctactgct ttttgaatag acaactcatt tgtttgcccg atattcgttc cattgatttg
     2041 aacagataga gcttggggat tcaatcgata acctttacat gtctgacaag ttaattctgt
     2101 catataaaga cgcatttgct cacgcgtgaa atcactgttc gtatcatgat aacgacgctc
     2161 gatattcttc aatacccctt cgaaagtcgt ttctacatcg cgaacgccac caaaatcatt
     2221 ttcataatgg aaatggaaaa gatcatctgt cccattcaag atgatctcct gatgctcttc
     2281 cggtaattct tcgaatggtg tatctaaatc gataccgaaa ctttcacaag cctgcgctaa
     2341 catttgagga taatactgag agctgattgg attccatgga acgatagcac cttgactaag
     2401 tgtcttcgtt ctatctggaa tcactaaatc ttgatcgact tctaatttga cacctaaacc
     2461 atcacagtct ggacaagctc caaaaggcgc attgaatgag aacagtcttg gttctagttc
     2521 gccaacagta aatccgcaat aaggacaggc atagtgttcg ctaaacaaca tttcttcttc
     2581 accgatcacg tcaaccagag catagccgtc tgctaatcgc agggccgcct ctaaagaatc
     2641 aaataggcga gaacgaatcc cttctttcac aatgatccgg tcgatgacga tcgcgatatc
     2701 atgttttttg tttttctcca gctcaggcac ttcacttaaa tcatagatct caccatcgac
     2761 tcgaacgcgg acgtatccct ctttttggat tcgttcgaat atttttttat gttgtccttt
     2821 tttctttacc acgatcggtg ccaacacttg gatctttgtg cgctcaggca aagccattac
     2881 ttgatctgcc atttgatcta ctgattgact ggtaatctcg atatgatcgt tgggacaaat
     2941 cggatgtcct acacgtgcaa acaacaagcg taaatagtca ttgatttccg tcacagtacc
     3001 tactgtcgac cgaggatttt tacttgttgt tttctgatca atagaaatcg ccggactcaa
     3061 tccgtcgatg ctatctacgt ccggcttatc catttgtcct aaaaactgac gagcataagc
     3121 agaaaggctc tctacgtatc tccgctgccc ttcagcgtac agcgtatcaa atgctaggga
     3181 actttttcca gaaccggaca aacctgtaac gaccacgaat ttatctcgtg gaatcgttac
     3241 atctatattt ttcaagttat gggcacgtgc accatgaatc gtaattttat cgttagccat
     3301 gttgtcctcc tactttgaag cttttaattc taaaatcata tctcgcacat tcgcggctgt
     3361 ttcaaaatcc aaagcttttg ccgcatcttt catttctttt tctaatttca tcaataacgt
     3421 gtctttttct tccttcgtca tttcttcgta cgagacttgt accgcttctt ttgtatcatc
     3481 ttccgattct ttcgtaatag aaatcaaatc tcggatttct ttaatgatcg tttttggaac
     3541 gatcccatgt tcttcattgt acttctgctg aatcgtacga cgtcttgacg tttcatccat
     3601 cgctagacgc atagaatctg ttactttgtc tgcgtacatg attactttac cttcttcatt
     3661 ccgagcagct cgtccaatcg tttggactaa ggaacgttca ctacgtaaaa agccttcttt
     3721 atccgcatcc aagatagcaa caagggaaac ttcaggtacg tctaatcctt cacgaagcaa
     3781 attgattcca actaacacat caaattcacc taatcgaaga tctcgaatga tttctgttcg
     3841 ttccagcgtt ttgatatcac tatgcaaata tttgactttg atccctaatt ctttgaaata
     3901 atctgtgagg tcttctgcca tttttttcgt caatgtcgtt acaaacaccc gctggtcttt
     3961 ttcgactcgt tcatttattt cgcctactag atcatcgatt tgtcccatga ttggacgaac
     4021 ttcgatcact gggtcaagca gccctgtcgg acggatgatt tgttggataa cggtatctgt
     4081 ttgttcatgt tcatatggtc ctggcgtcgc agagacataa atgatctgat tgacatgttt
     4141 ttcaaactct tctaaccgaa gtggccgatt gtctaaagca gatggcaaac ggaaaccgta
     4201 atctactagc atctgttttc tcgctcggtc accattatac attcctctga tttgaggcat
     4261 ggttacgtgc gattcgtccg ccacgatcaa aaaatcttca ggaaagaaat ccaatagcgt
     4321 ataaggcggt tcaccttctt tacgtccgtc catatgccga gaataatttt caattcccga
     4381 cgtgtagccc atttctaaca tcatttcaat atcgtaattg gttcgctgtt ctaaacgttg
     4441 agcttccaga agtttgtttt catttcgcaa gacagttaaa cgctgttcta attcctcttt
     4501 gatattcgct acggcatgct ccatatgttc atcattcgtc acaaagtgag tcgccgggaa
     4561 gattgctacg tgttccgttt ctcctaaaac ttcacctgtc agtgcattga cttctcggat
     4621 acgttcgacc tcatctccaa aaaactccac acgtaaggca cgttcatctc ttgaagccgg
     4681 aaaaatctcc acgacatcac cgcgcactcg aaaacgaccg cgctgaaaat ctatatcatt
     4741 tctttcgaat tggatactga ctaagtcacg aatcaactga ttgcgatcta gttctgctcc
     4801 ctgtcgaatc gaaactactt gtttttggta ctcaaaaggc gagcctaatc caaaaataca
     4861 tgaaacggat gcaatgacga tcacatcatt tctttctaat aatgaactag tagccgaatg
     4921 tcgtaatttg tcgatctcat catttacact tgaatctttt tcgatatacg tatcgcttga
     4981 aggcacataa gcttcaggct gataataatc ataataagaa acaaaatatt caacagcatt
     5041 gttagggaaa aattccttga attccccgta cagctgacca gccaatgttt tgttatgggc
     5101 aataatcagt gttggtttat tcactttctc aataagattg gaaatcgtat atgttttccc
     5161 tgttccagtc gcacctaata agatttgtgc ttttttcccg ccgactacgc catccactaa
     5221 ttggttaatc gcttcaggct gatcaccagc cggttggtat ttcgagacaa gctcaaagtg
     5281 tcgggaggta tctctctcaa tcattcttcg tcctcctttt cattcattcc ttttcatttt
     5341 acgaccaata aaaagaaaag gaaagcaaag gcattcctta ctaaaaaatt ataccacacc
     5401 gaacatactt tcgccacttt tttatcttat tatacatgtt atgttttata acataaaatg
     5461 ttagaaactc gcattttttt cattttttcg agcttacagt cttgtctgat tcagcacttt
     5521 tcgcctataa taataaaata ttggaaattt aggaggttga ttatgaaaag aaagcattta
     5581 tttcactcat ttgttttctt aatgacagga cttgtgacac ttgcgtttgg tcatgttgca
     5641 caagctgaca acactacaac ggcagagtca agcacaagcg agacaatgca aaaaatagag
     5701 cccaaaaaag acgtatacgt aattgccagt gactctgctt ttgctccatt tgaatttcaa
     5761 aacgcaaagg gcgagtacga aggcatcgat gtaaaattga tggaaaacat cgccaaactg
     5821 caaggattta acatccgaat ggactaccgt ggtttctctg ctgctttgca agctttggaa
     5881 tcaggacaag cagacggaat gattgccgga atgactgtaa cagatgaacg aaagaaaagc
     5941 tatgattttt ctgattctta ctttgatagc gggatccaat tagctgtcaa aaaaggagat
     6001 acttcgatca agtcttatga agatttgaaa ggaaaaacag tcggagcaaa agttggtacc
     6061 gaaagtgctg aatttttaag cgaccatgaa tctgaatatg gctattctat caaatatttt
     6121 gatgctgctg atcaattgta cgacgggtta aaagttggcg caatcgatgc aatgatggat
     6181 gattatccgg taatcggtta tgcgatcaaa caaggacaag aattggaaac accgatcaaa
     6241 aaagaatctg gcggaaaata tgcatttgcc gttaaaaaag gacaaaatcc tgagcttctt
     6301 aatatgttca atgaaggatt gaaagaatta aaaagaacag gcgaatacga taagattatt
     6361 aaagaatatg tagcggatgg ttcagaaact tccagtgagt cttccactgc tgatgaatca
     6421 acatttcttg gacttatcca aaacaactat caagcattgt tgaaaggact ttggagaaca
     6481 attctcctaa ctttgatctc ctttgctttg gctatagtaa tcggtgtgat ttttggatta
     6541 ttcagtgtag caccaatacg cgctcttcgc acaattgcta gtatttatgt cgatatcatc
     6601 cgaggaattc caatgatggt tcttgcgttc tttattttct ttgggttgcc tggtatcgtt
     6661 ggatttacga ttcctgactt ccttgctggt gttatcacct tgactcttaa cgcaagtgct
     6721 tatatcgcag agattgttcg aggcgggatc aacgccgttc ctgtcggaca aatggaagct
     6781 tccagaagtt taggtctttc ttataatcgg acaatgcaaa aaatcatttt gccgcaagcg
     6841 atacgcatca tgatcccatc attcgtcaat cagttcgtta tctcattaaa agacacaacg
     6901 atcatttccg caatcggtgt agtcgaattg ctgcaaactg gtaagatcat tgttgcacgt
     6961 acaacacaaa gtacgtatgt ttacttgatc attgcgatca tgtacttgat tttgatcaca
     7021 gcattgacaa aattagcaaa agttttagaa aagaaggtga agtaagatgg cagaaaaaat
     7081 cttagttgag catttagtca aaaaatacgg cgataacaca gtattaaacg acatcaatgt
     7141 atccatcaac gaaggagatg tcgtctgtat cattggccct tcaggttcag gaaaaagtac
     7201 attcttgcgt tgcttgaatc gtttagaaga accttctgac ggtgaaatta ttattgacgg
     7261 cgcccacttg atggataaaa gcacagatat caacttggtg agacaacata tcggaatggt
     7321 attccaacac tttaacctat tccctcatct ttctattttg gaaaacatca ttttagcacc
     7381 gatggacctg aagaaagaat ctagagaaga agcagagaaa aaagcaatca aattactgca
     7441 gacagttggt ctagaagata aaaaagatat gtatcctgaa atgctttctg gtggacaaaa
     7501 acagcgtgtg gctattgctc gtgctctagc gatgaaccct gacatcatgc tttttgatga
     7561 gcctacttct gcactcgatc ctgaaatggt cggtgatgtc ttgaacgtca tgaaaaagtt
     7621 agcagaacaa ggtatgacga tggttatcgt tacgcacgaa atggggttcg ctaaagaagt
     7681 ggccaatcgc gtcatgttca ttgacgacgg aaacttttta gaagatggaa cgcctcaaca
     7741 aatctttgaa aatccgcaaa atgaacggac aaaagatttc ttgaataaag tattaaatat
     7801 ttaaaataaa ttatatatgt ctttttgact atctttttat atcttcttaa atgtccgcaa
     7861 agccttattt tataggctct acgggcattt tgcttttgtg gtaaacctca catatctagg
     7921 tctatcttct tatattttca ctatcaagcg tggttaaaat cgtggtaaat acttctatgc
     7981 aattagacgc tgaacctctg attttgcggt atctatggac gcatgagcat accagttcat
     8041 tgtgatactg atatttgaat gccccatgat atactgtaaa tcttttgggt tcatgttctt
     8101 gcttgccagt cttgtgcaga atgtatggcg tagcgtatgc ggtgtgatat gtggcaatgg
     8161 attgtccttg tggtgcttgt tgtatttctt taccatacgg ataaataagg cgttataatc
     8221 aatcgcaact ttgggcttgc ccttatgatt gacaaatagg aagttgcccc gtccgtctat
     8281 cacaaatggt tctgcctttg gacgtttttt cataacctgt tgaaatgcct gtatcgtttc
     8341 tttgcttaat ggtacttgcc ttattccgct ttttgtttta ggcgtttcaa tataatagcc
     8401 ctgttccttg ctctttagta actggtggtc gataatcaca acttcattct tgaaatcaat
     8461 gtcggctact gtcagtccgc acagttccga aatacgaagt cctgtcttta acagtatcag
     8521 cacatcatca taatgcttgt gatacacatt gtccgtcttg atgaatgaga gtaaggcttg
     8581 ttcctgttcc tctgtcaatg cgactttctt tttggtatca ttttctagga cttcactcaa
     8641 cttgaaatca aagggatttt tcctcacata atcatcttga atggctatgt agaatgacgc
     8701 ttttaacgag cgtttatggt tgttaatggt attgtaggaa aagcctttgt ctttcatgcg
     8761 taacgcccat tccttagcgt cagagggttt gattgtatca atgctcctag cacctaactt
     8821 gtcctctttc aataaccgca tgagttgttc ccgttgtttc tgtgtgcttt tcttcacatt
     8881 tgccctctgt gcgttctgtt tggcataaag ttggcaaagc gtcattttct tgccagtgct
     8941 gtcgataccg tcctcaatat cccgtcttat ctgctgttcc agttctcgaa gtgagggttt
     9001 ttcccgtttt ccctttggtg tcgggtctgt gggtgtcaat ctccaagcat acacatattt
     9061 tgtgtttcca aatgcgtcta catatttata taagtatttt ctgtctgttc gttggctctc
     9121 tccagtatgc aggatacgac ctttgttatc ccgtcttttt tctttcatgg tgtctgctcc
     9181 tttccttgtt ggaaagagcc ttgatatgac ttgtgttcat cataacacat acaaggctca
     9241 tttgcattag atagcgtcca atttatcaat gacctgttca aactgtcggc gtttaatctg
     9301 tatgcggttg ccattcataa tgagccagcc agcgtccttg ttttcctctg ccaatcgtct
     9361 taacttgttt tctccgatac ggaaatactt tgacgcttct tcaatggtaa gggtgtattt
     9421 ttcccatacg ggaatatcct gtgcattact cataaaacac cccctcactt tcgattacgt
     9481 ctgctatgtc cgtacacatc tgttccacaa gcatttcctg cttttcagag aggtgggtac
     9541 attcatcaat cattttcatg tagtttgagc ctgtcagcct gtcgatttcc tctatcatca
     9601 cgattgacgg catgacctgt ctttccagcc attgcagagt gcgttccata ttgacaggta
     9661 cggggttcat ttcaaaaggt aaactgtcat gggaaatgaa tatctcccac agcggatagt
     9721 cgggaaattg cacaaaatca gtaataaggt aaaacaccag cccatgaggg ttatatgtca
     9781 ataacaattc ctcaactgcc tgtactgctt ttgtaccacg cagacgaatt tcaaaacgat
     9841 tgataatatc cgtatgtttc tttttggttg cctgttcctt ttgtttttca tacaggcaga
     9901 agtattttgt gcttgccttt gaaccgatat aaagggtgct tgctaaatta cggttttttc
     9961 cgcttagttt tccaccctgc acattttcat aatttttaca gcgacaatcc gcaccgccat
    10021 ttctgtattt ttcagacaaa acgggaatat ccaacagacc acacatatca ttgattgcca
    10081 agtcaaaacg tcggataaca ccgtcacagt caagacagcg gtttaaaaat gaataccaat
    10141 ctctgttctg tgcctgcaag acatattcca tgttgcgtga gcctgcccct tttaattcca
    10201 agaatacgcc catgtgttcg tcatcagaag ccatgacctt gatttctccg taggcgtaat
    10261 gctctttata gccataatac ccatagtcat aatggataaa ataatctgct ttcatgccaa
    10321 gcacctttcg gataatttcc aatgcgtcag tcgttggata acggacagac agataatcaa
    10381 cgagcagata aaaaggctta tcgcagatat aataattcag acaacgcagg ataatatttt
    10441 gtgtttcctt gctggcgttg gcttttccgt tctcaaagcg gttgatacta tatcttgata
    10501 catgggtaag acttgcaagt tctccttgtg aaagaccgct tgcaagacgt ttgttcttca
    10561 tttcaagtgc aaattctaaa ttgttcattg tattcctcca atcccacaat agccttgaaa
    10621 tacctgtaaa cctctattca aaaagtgtga tatgcactat cttcttaaaa cttctcatta
    10681 ctactcacaa ctttgggatt gtatggtttt tgacttgtct tttgtgaaat agtgcccccc
    10741 tgttagataa cgggggcaca gacaaggctc gcaggctcgc cagccgacaa agcatttctt
    10801 ccccttgaca caaggtgtca tggcggtaag cctgagaggc ttccgccacc gtgtcagaaa
    10861 tactttgtca gcaagttctg ttttgccttt tctaacaagt tccatttcaa ccgatattca
    10921 tatctacgga tttaatcaga taggtatttc tttttgaatg tttttatatt tcattctatt
    10981 gtgcttattc gattacaaga acatagtaat tcttatatgc ttatttgtca atatcttttt
    11041 ttgcctttac aaatcatatt aagcatgata gacttataat agcgaagata taacgagata
    11101 tggagggtta tgcaatgaaa ataagccatt taggaaataa tatacagacc ataagaaaat
    11161 ttaggggaat gaaacaacag gaacttgcgg acaaaatcgg tatcaatatg cagagccttt
    11221 ccaagattga aagagggttg aactatcctg cctatgaaac gctggaaaag ataatggaag
    11281 tgctggacgt tacgcccaat gaattgttgt cgggagaatg gaagtatgtc aatcaatctg
    11341 aaaaggaagt ctgtcagttt ttaagagccg aagaacgtct aaatgcagaa ctgaaacatg
    11401 gacactatga taacttcttt gacagcgagg aagaatggct ggaatatgag ttagaaaagt
    11461 tacgggaata tattacagac tatatcaacg ggaaaagcat tgaagcgtcc gacctttacc
    11521 caatcaaaga atttatccag catttgaagt ttcaaaaatt attagaccgc tatgatgatt
    11581 tatacagtat ggatatgttt ggggaaagca tagagggaca taagtataag acaccttatc
    11641 aagtcgtgaa aatgataaac ccgaactcaa aagaagatat ggaactgtta cgagaagtat
    11701 taaggaataa caattttgat gatgaggacg agtaactttt ttcctcttgc gagaagatac
    11761 agcaaacaaa aagcccgatc aagagtgcaa agcaccacca atggtggcaa agctcttgac
    11821 agggcttttt cttttctgtt gtgggcaatc aagaggaaga aagggataaa atctgatttg
    11881 tggtaaatca ggcaaaggca acgcaggtat ctcaatttga tttggtactt gaaaagaacg
    11941 ggagtaattg gaagattata gaataacaaa tattggtaca ttattacagc tattttgtaa
    12001 tcacgtactc tctttgataa aaaattggag attcctttac aaatatgctc ttacgtgcta
    12061 ttatttaagt atctatttaa aaggagttaa taaatatgcg gcaaggtatt cttaaataaa
    12121 ctgtcaattt gatagtggga acaaataatt ggatgtcctt ttttaggagg gcttagtttt
    12181 ttgtacccag tttaagaata cctttatcat gtgattctaa agtatccgga gaatatctgt
    12241 atgctttgta tgcctatggt tatgcataaa aatcccagtg ataaaagtat ttatcactgg
    12301 gatttttatg cccttttggg tttttgaatg gaggaaaatc acatgaaaat tattaatatt
    12361 ggagttttag ctcatgttga tgcaggaaaa actaccttaa cagaaagctt attatataac
    12421 agtggagcga ttacagaatt aggaagcgtg gacaaaggta caacgaggac ggataatacg
    12481 cttttagaac gtcagagagg aattacaatt cagacaggaa taacctcttt tcagtgggaa
    12541 aatacgaagg tgaacatcat agacacgcca ggacatatgg atttcttagc agaagtatat
    12601 cgttcattat cagttttaga tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa
    12661 gcacaaactc gtatattatt tcatgcactt aggaaaatgg ggattcccac aatctttttt
    12721 atcaataaga ttgaccaaaa tggaattgat ttatcaacgg tttatcagga tattaaagag
    12781 aaactttctg ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg
    12841 acgaacttta ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta
    12901 gagaaatata tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata
    12961 agatttcaga attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg
    13021 attgataacc ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg
    13081 tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca
    13141 tatatacgcc tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa
    13201 gaaaaaataa aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat
    13261 agagcttatt ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt
    13321 cttggagata caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta
    13381 caaacaactg ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg
    13441 gaaatctcag atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt
    13501 atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag
    13561 tatcatgtgg agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa
    13621 aatgcagaat ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt
    13681 ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt
    13741 ggatacttaa atcaatcgtt tcaaaatgca gttatggagg ggatacgcta tggctgtgaa
    13801 caaggattgt atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac
    13861 tatagccctg ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa
    13921 gtcttaaaaa aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg
    13981 ccacaggaat atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta
    14041 gacactcaat tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt
    14101 caagaatatc gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag
    14161 ttaaaagggt accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt
    14221 cggatagata aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat
    14281 ataaatatgg tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag
    14341 tgtaaaggag gagatagtta ttataaacta caagtggata ttgtgtcctg tatgtggaaa
    14401 taaaacacga ttaaagataa gggaagatac tgaattaaaa aaattccccc tctattgtcc
    14461 gaaatgcaga caagaaaatt taattgaaat aaagcagttc aaagtaactg tgattacaga
    14521 gccagacgca aagacgcaga gccgataaaa tgagattaat acaatctcat tttatcggct
    14581 ctttccgtta tgtatggatt cttttaatta gtcttcgatg tttcttgctt cgttgatacc
    14641 gctggctaaa gattccatta aggatagttc tttgtctgta aagctatcca tgtatttctc
    14701 tatctgtaat cgtcgggtgc tttttaccaa gttattagca ggtaagaaaa attcatcaac
    14761 ggaaacatga agtaacgata caaggtcata aagaacttgt atgctggggt gttgcccttt
    14821 attttcaata ttagttaagt accgtgggtc aatttcaatc aatgctccca cttgttcacg
    14881 agttaaacct cgtttcaatc gagcttcttt aatggctaaa ccaaaggctc taaaatcata
    14941 tttatcttct tttttacgca tagtagacca cctctataca ttttattgtt cctactgaat
    15001 taaaaacagg tatagaaaaa cgtgttatat ggtttatagg tttatattta ataaaaagca
    15061 ctactaaacg ccaataaaaa aaaccgttat atggtagtgc tatttacgct gttaaaatat
    15121 tgtatattac ttccaaatgg cgg
//
LOCUS       DAZKCC010000267         8062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.108, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000267 DAZKCC010000000
VERSION     DAZKCC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.108"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8VH_003174"
     CDS             128..1564
                     /locus_tag="ACG8VH_003174"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9111619.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8VH_003175"
     CDS             1753..4200
                     /locus_tag="ACG8VH_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9111620.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8VH_003176"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8VH_003176"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9111621.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8VH_003177"
     CDS             5487..5693
                     /locus_tag="ACG8VH_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111622.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8VH_003178"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8VH_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9111623.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8VH_003179"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8VH_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9111624.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAZKCC010000268         5437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.127, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000268 DAZKCC010000000
VERSION     DAZKCC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.127"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..459
                     /locus_tag="ACG8VH_003180"
                     /pseudo
     CDS             110..459
                     /locus_tag="ACG8VH_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(731..1021)
                     /locus_tag="ACG8VH_003181"
     CDS             complement(731..1021)
                     /locus_tag="ACG8VH_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111625.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1423..1659
                     /locus_tag="ACG8VH_003182"
     CDS             1423..1659
                     /locus_tag="ACG8VH_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111626.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1666..3618
                     /locus_tag="ACG8VH_003183"
     CDS             1666..3618
                     /locus_tag="ACG8VH_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9111627.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3690..4187)
                     /gene="dfrG"
                     /locus_tag="ACG8VH_003184"
     CDS             complement(3690..4187)
                     /gene="dfrG"
                     /locus_tag="ACG8VH_003184"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9111628.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4514..5206)
                     /locus_tag="ACG8VH_003185"
     CDS             complement(4514..5206)
                     /locus_tag="ACG8VH_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9111629.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1772 a          934 c          881 g         1850 t
ORIGIN      
        1 ctattatgtt tgttcatttt tatactcctt cttatatttc ctaatagagt ataacatagc
       61 tttaagcatt acttaaagat aacttaaaag ttatctttta aatacttaat tgttaaatac
      121 cttatttgat gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt atatccagaa
      181 aaatttttct tagttaagga cctatggatt gcagtaatgc agaattcgtc gaataaactg
      241 ccaaagacat acaatcatat tgcttttaaa attgacgaac aagatataga ttcgtttgtc
      301 tcaaaaatcc aaatgttagg tttaactgtc gaaccaggac gttctcgtgt gaaaggcgag
      361 gcttcttcaa tttactttta cgactatgat aatcacctct tcgagttaca taccggtact
      421 ctccaggaac gtttaaaatc ctacaattcc actacctgag actagtaatg actagtctct
      481 ttttatggcc tctgatcccc ccattgagcg aatactaata gggcttcgaa ttgatctgtt
      541 caatctttaa gcaatatccc tcaaatgagt ttggaaagtt tagtgattca tcacaaatac
      601 ttaaatacat gtagttatat atagattgag gttcttgtga gcgtatgctc gtgtgggttc
      661 aaatcccatc gtctgcatcc atctttccaa aacaagcggc taggacattt ttgtctcagc
      721 cgcttgtttt ctagtttatt ctttcatctt gtcggacctt ttttcttggg tatagacttt
      781 tctcatattc taccacaatt ttgggtttcc ctaacacatg ataggatctc acaacaaatt
      841 gctttctatt attataataa cctgtaacga ctaacttcat cccttcgtct acttcagcta
      901 ggaagttcaa actatgggaa gcaatcaaac aattgacccc atttaaactg aaacggatca
      961 acggtctggc actaacttgc aaagtacgga tcttatctac tgttccatat tttttctcca
     1021 taaccttcac ctcaatacca ttatacgaac atacattccc tttgtaaaga agaaactctg
     1081 ttttatcttt ttgacttttt gtctattacc tttcttaaaa tcatattggc acttcctgcg
     1141 aagatgaagg acatgtacta aaaaaaagag gcagcagtat agatttaagt agttcagttt
     1201 tggagtacaa aaagtgaaaa tgtaaaccta aatgagtatc aaattgcaac tgaatttgat
     1261 actcgttttt tattgttaaa tcaacgtttg ttgttaattt acaccacaaa atgtaagtta
     1321 aaatgtatat aaaaatgcga acgtgaaata attattgtag gaatgcaaat tggtgtgtta
     1381 aaatggttac aatttactaa gcaaaaaggg gtgcattttc ctatgcaatt tcttgagcca
     1441 aaaaataaga acgctaagtc tgtggattgg gaaatatcag agcaagtaag ggtgatagtt
     1501 aagcaatacg ctgaatatgc agagcgtact gaaagcgaag cagtggatga attcttattg
     1561 aacattttag acgataagaa gtttattgaa tggattgcca acaagcgaag taataagcgg
     1621 attgttgaaa aaatgggtat caaagataga gtgggttgat agacaatgac caagcttaaa
     1681 agattagcca cgaaggaaga tgaaattgta gatgttaaaa ttccgatttc aaacgaagaa
     1741 ttaaaagata gggctaaaca atatgattta ttaactccaa aacgatttgc aacaaggtat
     1801 aacaaaatgt tgttcttgcc aacgtctttt aaatggaacg gttctgaata tcccattcaa
     1861 tataattact gcatcaatcc gttttgttgt aattttggga aagaacagca caaattcaaa
     1921 gatgtaaaag ggaaaccaag tcgatataaa atgactggta gttccaaaga taaaggtcat
     1981 aaaggtatgt actgcaatga caatcctatt ggcagaggtg tatcgcaaaa ctgtacagta
     2041 acccctttat ctaattggtc tgttgttgaa gaaattaaac gccttataga aatcaatagc
     2101 atccaagatg ttgagccaga ttatcaattt cataaagaag gctgctcgga agaagaatca
     2161 accccattca atgagccgaa gcaattttac aaaaggggga aaagtagggg caaatctcaa
     2221 cgctatcaat gtaaggcgtg taagaagttc actaatgttc taccaaaaag agaagaaacc
     2281 actacttatc accaacaaaa gaacaccatt cttccaatgt tcgctaagat ggttgttgga
     2341 agggtttcag tcagtcgtac ttgtgatatt ttaggaattg gagttggaac ctactatcat
     2401 aagctggagt ggctgtatag gcgttgtttg gagtttttag aacgatatga gacacaaccc
     2461 cttcaaacaa agaaatttaa cgagatgtgg cttaatacgg acaagatgca ctactaccta
     2521 aataacgtcc gtaagaaagg acaaggttca aagaaatata cggggtttga agaccttaat
     2581 atgcaaacgt atatagttgt atctgctgag gtcttatcaa gatatgtgtt ccgttctgat
     2641 gttgcctatg attggaatat ttcaatggat gaattaaatg aagatacaag aaaattcaaa
     2701 gaagaccatc tcaatacttt tagtcgtaaa aatgataggt tagattggtc ttattatcca
     2761 caagaaccat ccgctaacga tagcgaaaat aggaatgctt atcttcacga attagggaaa
     2821 attactaata ggagtagatt tgtcgatggt ttaaatgttg atgctcctta tactacaact
     2881 gctcactatt ggttaattaa acaaatggtt aatgccgacg aatggcgaat gattagtgat
     2941 gatgacttct ctattagaaa cgccttttat agagtattca cgaaagaatt aaggttgagc
     3001 gatgcacatc attttatttg ccaagttaac aagacgaaaa gcagaaaaca atgcttaaaa
     3061 gaattcggac aagcaaaagc agaactgctc gattggggag atatcagagg attcaaaaca
     3121 aagtttctac ggactttggc atctcattat ctaactgaat tactaacaag tcatcaattc
     3181 cacgaagagg ctattagtaa agatggagaa agatatcgca aatatgccga taaccctatc
     3241 aaacatccat tagccactaa ggataaagga ttttattcag ttgattgtag aacggattta
     3301 tctgctctgg aaccaaacga aatcgcaaag atgctattaa atgttaatga ccattcgacc
     3361 aatagcttta ttcaacagat aagacgttat atatcatctc ttgaaagacc attaacaaca
     3421 gctcgtggtg ataagaagag ctatatttac gctaacttca atccgaagta tgctcaattt
     3481 gcgattacaa ttctaagaac ttactacaac ttttgtcgcc cttttaaatc agcggataaa
     3541 aaggttttga cacctgctca aagattaggg attacagata aacaatttga ttggaaagat
     3601 ataatttatt tcaaatgaca aaaagagctt ggagtaatcc caagctcttg tgctttatct
     3661 ttactgttct tcttcaatta acgcacccgt taactcaata agttttttct ttcatataca
     3721 tgaaaatagt agttatacgg atttttatca ttctttatcc ctttttgggc aaatacctca
     3781 ttccattcct cataattcac ttctggaaaa aaagtatctc cttcgaattc atgatgtatt
     3841 tttgtgatgt acattttctc aacataaggg aaaaacaaat tataaatctg ttctcctccg
     3901 aaaataaaaa tttcttcttc gtttttacat aactcaaaaa catcttctat tgaatgaaca
     3961 atttcacaac cattaaaggt aaacccctta tctctcgtca gaataatatt tcttctgtca
     4021 ggtaaggctc ttccgattga ttcaaggttc ttcctaccta atattatcgg atgtcccttt
     4081 gtagtatttt taacatattc ccagtccttg ggaatcctcc aaggaatgtc attctctttg
     4141 ccaatcactc tattcttatc catcgcagca atcaaagaaa ctttcattta taacacagcc
     4201 tttttaacta atttaccatc taatttctta tttctatcaa aaaacctttt ctcattatcc
     4261 tgcccgttag ttgaataagc aattttttct ataagttata ttatcataat accacaaaat
     4321 cacaaatttt cattcaatta tcattttcaa ttgcatttta taaatcattt tgtatgtaat
     4381 ttggtatgta agttaaaatg aaaaaagtag tccattttga agtacatttt gtcattttgt
     4441 actccaaaac tgaactactt agtatagatt actgttgctt ctttactaat ttaggtttac
     4501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     4561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     4621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt tctacatttg
     4681 ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta ttttgtaatt
     4741 ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc agatgattga
     4801 tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt tgtttgcttt
     4861 gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct gctgccgatt
     4921 gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca tctgcctttg
     4981 aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta atcgcttgct
     5041 ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta tcaatatctg
     5101 tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag ccaattagac
     5161 atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc atagctcctt
     5221 aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca agcgttttta
     5281 aattacatat gtaattttaa tataaatact atcaaatact catttaatcc tcctttttta
     5341 tctatttaaa aaatcttttt tatagagaaa aatgcaggtt aatactttct taaataaata
     5401 tttatggtga acaaaaacaa aaaagcaagt tttatta
//
LOCUS       DAZKCC010000269         5111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.134, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000269 DAZKCC010000000
VERSION     DAZKCC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.134"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..722
                     /locus_tag="ACG8VH_003186"
     CDS             75..722
                     /locus_tag="ACG8VH_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFP9111630.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1415..2353)
                     /locus_tag="ACG8VH_003187"
                     /pseudo
     CDS             complement(1415..2353)
                     /locus_tag="ACG8VH_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2353..2970)
                     /locus_tag="ACG8VH_003188"
     CDS             complement(2353..2970)
                     /locus_tag="ACG8VH_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9111631.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2984..3154)
                     /locus_tag="ACG8VH_003189"
     CDS             complement(2984..3154)
                     /locus_tag="ACG8VH_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111632.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3502..4992)
                     /locus_tag="ACG8VH_003190"
     CDS             complement(3502..4992)
                     /locus_tag="ACG8VH_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9111633.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
BASE COUNT         1571 a          890 c          794 g         1856 t
ORIGIN      
        1 gacataacgt atgaaaaaat cagaggatta ttcctcctaa atataaaaat ttaaaattta
       61 ggaggaagtt atatatgact tttaatatta ttgaattaga aaattgggat agaaaagaat
      121 attttgaaca ctattttaat cagcaaacta cttatagcat tactaaagaa attgatatta
      181 ctttgtttaa agatatgata aaaaagaaag gatatgaaat ttatccctct ttaatttatg
      241 caattatgga agttgtaaat aaaaataaag tgtttagaac aggaattaat agtgagaata
      301 aattaggtta ttgggataag ttaaatcctt tgtatacagt ttttaataag caaactgaaa
      361 aatttactaa catttggact gaatctgata aaaacttcat ttctttttat aataattata
      421 aaaatgactt gcttgaatat aaagataaag aagaaatgtt tcctaaaaaa ccgatacctg
      481 aaaacaccat accgatttca atgattcctt ggattgattt tagttcattt aatttaaata
      541 ttggtaacaa tagcagcttt ttattgccta ttattacgat aggtaaattt tatagtgaga
      601 ataataaaat ttatatacca gttgctctgc aacttcatca ttctgtatgt gatggttacc
      661 atgcttcact atttatgaat gaatttcaag atataattca tagggtagat gattggattt
      721 agtttttaga ttttgaaagt gaatttaatt ttatacacgt aagtgatcat aaaatttatg
      781 aacgtataac aaccacattt tttggttgct tgtggttttg attttgaatt tggttttgaa
      841 cttatggact gatttattca gtccattttt tgtgcttgca caaaaactag cctcgcagag
      901 cacacgcatt aatgacttat gaaacgtagt aaataagtct agtgtgttat actttacttg
      961 gaagatgcac cgaataaaaa atattgaaga acaactagca aaagatttta aagagttatt
     1021 ttattttaag tctttataac atgagtgaag cgaattttta aatttcgata gaaattttta
     1081 catcaaaaag ccccctgtca aaattgacga agggggtttt ttggcgcacg cttttcgtta
     1141 gaaatataca agattgaaaa tcgtgtataa gtgcgccctt tgttttgaac ttagcacgtt
     1201 acatcaattt tttaaaatga tgtataagtg cgccctttta aattttgagt gattatattt
     1261 tttgagttag aaaaagggat tgggaaaatt tcccaaaata atttaaaaaa taagcaaaaa
     1321 ttttcgatag agaatgtgct attttttgtc aaaggtgtat accttgactg tgcttgctgt
     1381 tacattaagt ttatttttaa gttattaaaa aagaaatagc ttttaaagtt tggctcgctg
     1441 tcgctttata aagctgattg acttttgatt gcaaactact taaagaaaac aaactcggac
     1501 tattcgtttt cttctctttg gtttgaacat cagcaattat cccctcttga ttgcctattt
     1561 tagcttgttt agaagaaaca aaagctaaaa gctcctcttg ggttttaaaa cgctgtgtgg
     1621 ggcttagaac gcccttaaac gacccttggt ttacttttat actagcttcc acctcgaaaa
     1681 aaggttcttt tttaaaattc tctatggctt cctggcgctg aaaaataagg tataaggtgg
     1741 gcgtttgaac acgtcctagt gaaaatgtac cttgtacgcc cttctgttgt aaatttaacg
     1801 tatacaaagg gcttgcgttc atgccgatca accaatcggc aatttggcgt gtttgcgctt
     1861 cttgataaaa gggatagtaa ttcattccag gttgcaaatt ttgaaaaccg cttcggatta
     1921 catctttttc taagctattg atccatagtc ttttaaatgt tttatctttt gaaaaggcat
     1981 ttgctttatg gataatcgac caggcgatat tttcaccttc tctgtcgcta tctgttgcaa
     2041 caataattgt atttgccttt ttgagaagtt ctgcaacaat tttaaactgc tttcccttat
     2101 cttttgcaac ttcaaaatcg tatcgatcag gaaaaatcgg caaagattca agtttccaat
     2161 tttgccactt ttcgtcataa tgacctggtt ctgctaattc cactaaatgc ccaaaaccaa
     2221 aggtgataaa cgtttcatct gtaaatagtg ggtctttgat ctcaaaataa ccgtcttttt
     2281 tggtgctttg ttttaaagca cttgcgtagg ctaatgcctg gcttggtttt tcagctaaaa
     2341 taaccgtact cattaactat ccctcttttc attgtttttt ctttgatcga ctgtcacgtt
     2401 atatcttgct cgataccttc taaacgttcg gcgattgatt ccagtttgtt cttcaacttc
     2461 tttatcggat aaaccattca aaaacaaatc gaaagcatgc tttaaacgtg gatcattttc
     2521 tttaaattta tgctgacgac ctttgaattt gccttttttc ttagcaattt cgattccttg
     2581 tgcctgacgt tccttaattt tttttcgttc tgattctgct tgatacttgt acaattcaat
     2641 gacaaggcta ttaatcaaac gccttaaatt ttcatcttca ataccattca ttgagggtaa
     2701 atttaagact tccagggttg cccccttaat ttgaatttga ttcatcaatt ctgttaattc
     2761 tttattattt cgtcctaatc gatctaattc agtaacaata acaatatccc cttcacgaat
     2821 atagttaagc atagcttgta attgtgggcg ttcgaccgat tgaccgctta atttgtctga
     2881 aaagacctta gaaacgccct gtaacgcttg taattgccga tctaagttct gttctttgct
     2941 actgacacgt gcataaccaa ttttagccat tttcaaccaa cctctaaaat tctctcggtt
     3001 gcaataacca atcagcaata tctacttttt caatttcaaa ttgcttatca gaaattgtct
     3061 tttcgtaagc gataaaatct tgcgcatatt gttgctcatt aaaaatagcc accacttcgt
     3121 cattttctaa aactcgataa ataaattttt tcattttact cctcctatta tgcccaactt
     3181 aaatgaccta ttcaccaagt caattatact gctaaaatca tattaggaca aataggtata
     3241 ctctaatgac ctataaaaga tagaaaatta aaaaatcaag tgttcgcttc gctctcactg
     3301 cccctcgacg ttttagtagc ctttccctca cttcgttcag tccaagccaa ctaaaagttt
     3361 tcgggctact ctctccttct ccccctaata attaattaaa atcttactct gtatatttct
     3421 gctaatcatt cgctaaacag caaagaaaaa acaaacacgt atcatagata taaatgtaat
     3481 ggcatagtgc gggttttatt ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc
     3541 cgttttaggg cgttctgcta gcttgtttaa agtctcttga atgaatgtat gctctaagtc
     3601 aaaagaattt gtcagcgcct ttatatagct ttctttttct tcttttttta ctttaatgat
     3661 cgatagcaac aatgatttaa cactagcaag ttgaatgcca ccatttcttc ctggtttaat
     3721 cttaaagaaa atttcctgat tcgccttcag taccttcagc aatttatcta atgtccgttc
     3781 aggaatgcct agcacttctc taatctcttt tttggtcgtc actaaataag gcttgtatac
     3841 atcgcttttt tcgctaatat aagccattaa atcttctttc cattctgaca aatgaacacg
     3901 ttgacgttcg cttctttttt tcttgaattt aaaccaccct tgacggacaa ataaatcttt
     3961 actggttaaa tcacttgata cccaagcttt gcaaagaatg gtaatgtatt ccctattagc
     4021 cccttgatag ttttctgaat aggcacttct aacaattttg attacttctt tttcttctaa
     4081 gggttgatct aatcgattat taaactcaaa catattatat tcgcacgttt cgattgaata
     4141 gcctgaacta aagtaggcta aagagagggt aaacatgacg ttattacgcc ctattaaacc
     4201 cttttctcct gaaaatttcg tttcgtgcaa taagagatta aaccagggtt catctacttg
     4261 ttttttgcct tctgtaccgc ttaaaaccgt tagacttgaa cgagtaaagc ccttattatc
     4321 tgtttgtttg aaagaccaat cttgccattc tttgaaagaa taacggtaat taggatcaaa
     4381 aaattctaca ttgtccgttc ttggtatgcg agcaatacca aaatgattac acgttagatc
     4441 aactggcaaa gactttccaa aatattctcg gatattttgc gaaattattt tggctgcttt
     4501 gacagattta aattctgatt ttgaagtcac atagactggc gtttctaaaa caaaatatgc
     4561 ttgataacct ttatcagatt tgataatcat agtaggcata aaacctaaat caatagcggt
     4621 tgttaaaata tcgcttgctg aaatagtttc ttttgccgtg tgaatatcaa aatcaataaa
     4681 gaaggtattg atttgtctta aattgttttc agaatgtcct ttcgtgtatg aacggttttc
     4741 gtctgcatac gttccataac gataaacgtt gggtgtccaa tgtgtaaatg tatcttgatt
     4801 ttcttgaatc gcttcctcgg aagtcagaac aacaccacga ccgccaatca tgcttgattt
     4861 tgagcgatac gcaaaaatag cccctttgct tttacctggc ttggtagtga ttgagcgaat
     4921 tttactattt ttaaatttgt actttaacaa gccgtcatga agcacagttt ctacaacaaa
     4981 agggatattc attcagctgt tctcctttcc tataaatcct ataaaatagg ttgtttaatt
     5041 aacttggttt gctttttcat tcaactgttt caatattgca tgttttgaaa aagatttttt
     5101 tcctttataa g
//
LOCUS       DAZKCC010000270         4243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.146, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000270 DAZKCC010000000
VERSION     DAZKCC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.146"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..1671
                     /gene="msr(C)"
                     /locus_tag="ACG8VH_003191"
     CDS             193..1671
                     /gene="msr(C)"
                     /locus_tag="ACG8VH_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9111634.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1718..2164
                     /locus_tag="ACG8VH_003192"
     CDS             1718..2164
                     /locus_tag="ACG8VH_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9111635.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2363..2878)
                     /locus_tag="ACG8VH_003193"
     CDS             complement(2363..2878)
                     /locus_tag="ACG8VH_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9111636.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2995..4197
                     /locus_tag="ACG8VH_003194"
     CDS             2995..4197
                     /locus_tag="ACG8VH_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9111637.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1262 a          715 c          926 g         1340 t
ORIGIN      
        1 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
       61 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
      121 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
      181 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
      241 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
      301 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
      361 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
      421 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
      481 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
      541 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
      601 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
      661 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
      721 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
      781 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
      841 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
      901 caaatcagac tggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
      961 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
     1021 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
     1081 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
     1141 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
     1201 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
     1261 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
     1321 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
     1381 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
     1441 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
     1501 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
     1561 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
     1621 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
     1681 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
     1741 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
     1801 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
     1861 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
     1921 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
     1981 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
     2041 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
     2101 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
     2161 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
     2221 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
     2281 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
     2341 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
     2401 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
     2461 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
     2521 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
     2581 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
     2641 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
     2701 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
     2761 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
     2821 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
     2881 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
     2941 tgtgcatatt gacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
     3001 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
     3061 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
     3121 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
     3181 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
     3241 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
     3301 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
     3361 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
     3421 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
     3481 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
     3541 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
     3601 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
     3661 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
     3721 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
     3781 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
     3841 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
     3901 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
     3961 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
     4021 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
     4081 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
     4141 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
     4201 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DAZKCC010000271         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.162, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000271 DAZKCC010000000
VERSION     DAZKCC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.162"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG8VH_003195"
     CDS             3..188
                     /locus_tag="ACG8VH_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9111638.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG8VH_003196"
     CDS             203..1072
                     /locus_tag="ACG8VH_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9111639.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG8VH_003197"
     CDS             1053..1787
                     /locus_tag="ACG8VH_003197"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9111640.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG8VH_003198"
     CDS             1820..2728
                     /locus_tag="ACG8VH_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9111641.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACG8VH_003199"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACG8VH_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKCC010000272         3084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000272 DAZKCC010000000
VERSION     DAZKCC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.165"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..1056
                     /locus_tag="ACG8VH_003200"
     CDS             145..1056
                     /locus_tag="ACG8VH_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFP9111642.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1209..1694
                     /gene="vanZ-A"
                     /locus_tag="ACG8VH_003201"
     CDS             1209..1694
                     /gene="vanZ-A"
                     /locus_tag="ACG8VH_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9111643.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2213..2497
                     /locus_tag="ACG8VH_003202"
     CDS             2213..2497
                     /locus_tag="ACG8VH_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9111644.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2863..>3084
                     /locus_tag="ACG8VH_003203"
     CDS             2863..>3084
                     /locus_tag="ACG8VH_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9111645.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKL"
BASE COUNT         1158 a          424 c          569 g          933 t
ORIGIN      
        1 taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa aacggcacaa
       61 tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat acttccaact
      121 atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt attgttattc
      181 ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga aaaagtcgaa
      241 tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac ttctgaaaat
      301 acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct attaatcaat
      361 agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt atctaaacat
      421 gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc aaaagaaata
      481 gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag tcattttatt
      541 attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga aatgggggct
      601 gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact agatgtagga
      661 tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga aaatgcttgg
      721 aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg aattcaatat
      781 gaaccatggc atattcgcta tgttggttta ccacatagtg cgattatgaa agaaaagaat
      841 ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc tgttagtgta
      901 aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac cattcatgtg
      961 ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat tgtgacagtg
     1021 tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga aaccaacgaa
     1081 attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt ttaatttaaa
     1141 acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa gaattagagg
     1201 aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta gtgacactaa
     1261 tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat tatcatcaaa
     1321 gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg atagataatg
     1381 ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa atcggatttt
     1441 tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt caatttatct
     1501 tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga ggctttcttg
     1561 gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta gacagagtta
     1621 ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc catttaagaa
     1681 taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca taaagatatt
     1741 gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag gtgtgaagtg
     1801 aggggaaaac cggtgataaa gtaaaaagct tacctaacac tatagtaaca aagaaagccc
     1861 aattatcaat tttagtgctg aggaattggt ctctttaata aatttcctta acgttgtaaa
     1921 tccgcatttt cctgacggta cccccactag cagccatgct ttttttgaat ctttgtagga
     1981 cgttttaaag agaattaccc actttaaaat taaacatgcg cttagaatcg ctttaggaaa
     2041 cacgatccag tccaataatc gtcgataaaa acttttgaaa aaggttggtg aaattaccta
     2101 cttttggaat aatcacaaat cacaagtgat taatcacttg tttattaaga tattaaaagc
     2161 tataatttaa ataaagcgtg aattttatta cacaaaaaga ggggggagaa acttggaact
     2221 agcatttaga gaaagcttaa aaaagatgag aggtaccaaa tcaaaagaaa aattctccca
     2281 agaattagaa atgagtagat caaattattc acgaatagaa tcaggaaaat cagatccaac
     2341 cataaaaaca ctagaacaaa ttgcaaagtt aactaactca acgctagtag tggatttaat
     2401 cccaaatgag ccaacagaac cagaaccaga aacagaatca gaacaagtaa cattggattt
     2461 agaaatggaa gaagaaaaaa gcaatgactt cgtgtgaata atgcacgaaa tcgttgctta
     2521 ttttttttaa aagcggtata ctagatataa cgaaacaacg aactgaatag aaacgaaaaa
     2581 agagccatga cacatttata aaatgtttga cgacatttta taaatgcata gcccgataag
     2641 attgccaaac caacgcttat cagttagtca gatgaactct tccctcgtaa gaagttattt
     2701 aattaacttt gtttgaagac ggtatataac cgtactatca ttatataggg aaatcagaga
     2761 gttttcaagt atctaagcta ctgaatttaa gaattgttaa gcaatcaatc ggaaatcgtt
     2821 tgattgcttt ttttgtattc atttatagaa ggtggagttt gtatgaatca tgatgaatgt
     2881 aaaacttata taaaaaatag tttattggag ataagaaaat tagcaaatat ctatacacta
     2941 gaaacgttta agaaagagtt agaaaagaga aatatctact tagaaacaaa atcagataag
     3001 tatttttctt cggaggggga agattatata tataagttaa tagaaaataa caaaataatt
     3061 tattcgatta gtggaaaaaa attg
//
LOCUS       DAZKCC010000273         2810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.170, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000273 DAZKCC010000000
VERSION     DAZKCC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.170"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..1108
                     /locus_tag="ACG8VH_003204"
     CDS             140..1108
                     /locus_tag="ACG8VH_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9111646.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1101..2132
                     /gene="vanA"
                     /locus_tag="ACG8VH_003205"
     CDS             1101..2132
                     /gene="vanA"
                     /locus_tag="ACG8VH_003205"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9111647.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2138..2746
                     /locus_tag="ACG8VH_003206"
     CDS             2138..2746
                     /locus_tag="ACG8VH_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9111648.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          828 a          546 c          692 g          744 t
ORIGIN      
        1 agaaacggct cgttcttaca cggtagactt aataccgtaa gaacgagccg ttttcgttct
       61 tcagagaaag atttgacaag attaccattg gcatccccgt tttatttggt gcctttcaca
      121 gaaagggttg gtcttaatta tgaataacat cggcattact gtttatggat gtgagcagga
      181 tgaggcagat gcattccatg ctctttcgcc tcgctttggc gttatggcaa cgataattaa
      241 cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg
      301 acataaatca gagatttccg cctctattct tcttgcgctg aagagagccg gtgtgaaata
      361 tatttctacc cgaagcatcg gctgcaatca tatagataca actgctgcta agagaatggg
      421 catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt gccgattata ctatgatgct
      481 aattcttatg gcagtacgca acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt
      541 caggttggac agcgaccgtg gcaaggtact cagcgacatg acagttggtg tggtgggaac
      601 gggccagata ggcaaagcgg ttattgagcg gctgcgagga tttggatgta aagtgttggc
      661 ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa
      721 tagcgatatc gttacgcttc atgtgccgct caatacggat acgcactata ttatcagcca
      781 cgaacaaata cagagaatga agcaaggagc atttcttatc aatactgggc gcggtccact
      841 tgtagatacc tatgagttgg ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt
      901 ggatgtattg gaaggagagg aagagttttt ctactctgat tgcacccaaa aaccaattga
      961 taatcaattt ttacttaaac ttcaaagaat gcctaacgtg ataatcacac cgcatacggc
     1021 ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa accattaaaa actgtttgga
     1081 ttttgaaagg agacaggagc atgaatagaa taaaagttgc aatactgttt gggggttgct
     1141 cagaggagca tgacgtatcg gtaaaatctg caatagagat agccgctaac attaataaag
     1201 aaaaatacga gccgttatac attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa
     1261 aaccttgcgc ggaatgggaa aacgacaatt gctattcagc tgtactctcg ccggataaaa
     1321 aaatgcacgg attacttgtt aaaaagaacc atgaatatga aatcaaccat gttgatgtag
     1381 cattttcagc tttgcatggc aagtcaggtg aagatggatc catacaaggt ctgtttgaat
     1441 tgtccggtat cccttttgta ggctgcgata ttcaaagctc agcaatttgt atggacaaat
     1501 cgttgacata catcgttgcg aaaaatgctg ggatagctac tcccgccttt tgggttatta
     1561 ataaagatga taggccggtg gcagctacgt ttacctatcc tgtttttgtt aagccggcgc
     1621 gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa
     1681 ttgaatcggc aagacaatat gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg
     1741 aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa
     1801 tcaggctgca gtacggaatc tttcgtattc atcaggaagt cgagccggaa aaaggctctg
     1861 aaaacgcagt tataaccgtt cccgcagacc tttcagcaga ggagcgagga cggatacagg
     1921 aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg tctagcccgt gtggatatgt
     1981 ttttacaaga taacggccgc attgtactga acgaagtcaa tactctgccc ggtttcacgt
     2041 catacagtcg ttatccccgt atgatggccg ctgcaggtat tgcacttccc gaactgattg
     2101 accgcttgat cgtattagcg ttaaaggggt gataagcatg gaaataggat ttactttttt
     2161 agatgaaata gtacacggtg ttcgttggga cgctaaatat gccacttggg ataatttcac
     2221 cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta gggacatacg agttggctga
     2281 atcgcttttg aaggcaaaag aactggctgc tacccaaggg tacggattgc ttctatggga
     2341 cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa
     2401 taacctgaca aaggaaagtt attatcccaa tattgaccga actgagatga tttcaaaagg
     2461 atacgtggct tcaaaatcaa gccatagccg cggcagtgcc attgatctta cgctttatcg
     2521 attagacacg ggtgagcttg taccaatggg gagccgattt gattttatgg atgaacgctc
     2581 tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc
     2641 catcatggaa aacagtgggt ttgaagcata tagcctcgaa tggtggcact atgtattaag
     2701 agacgaacca taccccaata gctattttga tttccccgtt aaataaactt ttaaccgttg
     2761 cacggacaaa cggttctgtt gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DAZKCC010000274         1991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.186, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000274 DAZKCC010000000
VERSION     DAZKCC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.186"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8VH_003207"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8VH_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9111649.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8VH_003208"
     CDS             763..1917
                     /locus_tag="ACG8VH_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9111650.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          652 a          375 c          427 g          537 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat t
//
LOCUS       DAZKCC010000275         1991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.187, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000275 DAZKCC010000000
VERSION     DAZKCC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.187"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8VH_003209"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8VH_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9111651.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /gene="vanS"
                     /locus_tag="ACG8VH_003210"
     CDS             763..1917
                     /gene="vanS"
                     /locus_tag="ACG8VH_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFP9111652.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGI
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          651 a          375 c          427 g          538 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gatacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat t
//
LOCUS       DAZKCC010000276         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4312
            SAMEA115707090-rid22580043.guided.216, whole genome shotgun
            sequence.
ACCESSION   DAZKCC010000276 DAZKCC010000000
VERSION     DAZKCC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707090
            Sequence Read Archive: ERR13196822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287875.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287875.1
            Genome Coverage       :: 153x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:37:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,212
            CDSs (total)                      :: 3,151
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 14 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707090-rid22580043.guided.216"
                     /isolate="VRE4312"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG8VH_003211"
     CDS             26..76
                     /locus_tag="ACG8VH_003211"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9111653.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8VH_003212"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8VH_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9111654.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
